CN110279392A - A kind of fundus fluorescence imaging system and method - Google Patents
A kind of fundus fluorescence imaging system and method Download PDFInfo
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- CN110279392A CN110279392A CN201910505572.8A CN201910505572A CN110279392A CN 110279392 A CN110279392 A CN 110279392A CN 201910505572 A CN201910505572 A CN 201910505572A CN 110279392 A CN110279392 A CN 110279392A
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- fundus
- infrared
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- light source
- camera
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B3/00—Apparatus for testing the eyes; Instruments for examining the eyes
- A61B3/10—Objective types, i.e. instruments for examining the eyes independent of the patients' perceptions or reactions
- A61B3/12—Objective types, i.e. instruments for examining the eyes independent of the patients' perceptions or reactions for looking at the eye fundus, e.g. ophthalmoscopes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B3/00—Apparatus for testing the eyes; Instruments for examining the eyes
- A61B3/10—Objective types, i.e. instruments for examining the eyes independent of the patients' perceptions or reactions
- A61B3/14—Arrangements specially adapted for eye photography
Abstract
The present invention relates to a kind of fundus fluorescence imaging systems, including fundus imaging apparatus (1), the fundus imaging apparatus (1) includes fundus camera (4), the fundus camera (4) includes light source, optical sensing element and camera lens, the light source and camera lens are coaxial, the operating mode of the light source includes two area's light source mode of near-infrared, the optical sensing element includes two area's sensitive fluorescent part of near-infrared, when work, the fluorescein on two area light source activation measurand eyeground of near-infrared, two area's sensitive fluorescent part of near-infrared receives two area's fluorescence of near-infrared that measurand fundus fluorescence element generates and is imaged.Compared with prior art, the high-resolution fast imaging of eyeground deep tissue may be implemented in the present invention, enhances the effect of fluorescence imaging method.
Description
Technical field
The present invention relates to fundus imaging technical fields, more particularly, to a kind of fundus fluorescence imaging system and method.
Background technique
Eyeground is that whole body uniquely passes through eyes and can see the position of blood vessel, and many whole bodies and fundus oculi disease have on eyeground
Feature sex expression can be directly observed the capillary and optic nerve being distributed on eye ground by fundus angiography inspection
Structure, and then convenient for carrying out the diagnosis of disease.Be now widely used for retinopathy diagnosis camera shooting method include eye-ground photography,
More/ultraphotic spectrum fundus angiography and fundus fluorescence camera shooting etc., respectively there is certain deficiency in these inspection methods.
Eye-ground photography method obtains the color image on eyeground surface by radiation of visible light eyeground, and this method is simple and convenient,
Using relatively broad.But since penetration power of the visible light of use to fundus tissue is limited, it is difficult to obtain deep tissues (such as
Choroid) lesion information, to optical fundus blood vessel move towards differentiate help it is limited.In order to expand the function of common fundus camera, more/
Ultraphotic composes the fundus reflex image under multiple (the being more than RGB) spectrum of fundus angiography method acquisition, obtains eyeground surface and depth
The structural information of layer tissue, the information provided by this method acquisition is more abundant, can obtain some information of deep tissues,
But the signal of acquisition is also based on optical reflective information, can not be clearly observed tiny tissue and blood vessel.Fundus fluorescence
Camera shooting is to inject blood circulation system using specific fluorescein (fluorescein sodium or indocyanine-green etc.), by connecing when optical fundus blood vessel
The light excitation for receiving specific wavelength issues fluorescence, so that optical fundus blood vessel clearly image is obtained, inspection of this method based on active fluorescence
It surveys, clarity is high, is the important indicator that fundus oculi disease diagnoses " goldstandard " and treatment and evaluation prognosis.But due to exciting light
Wavelength is in visible-range (488 nanometers and 795 nanometers), and penetration power is not deep enough, therefore this method is in bleeding inflammation
With the agglomerates of pigments accumulation and melanoma problem in degenerative process, diabetic retinopathy, caused by retinal vein obstruction
No perfusion area etc. cannot all be imaged well.
Summary of the invention
It is an object of the present invention to overcome the above-mentioned drawbacks of the prior art and provide a kind of fundus fluorescences to be imaged
System and method.
The purpose of the present invention can be achieved through the following technical solutions:
A kind of fundus fluorescence imaging system, including fundus imaging apparatus, the fundus imaging apparatus include fundus camera, institute
Stating fundus camera includes light source, optical sensing element and camera lens, and the light source and camera lens are coaxial, the operating mode packet of the light source
Including two area's light source mode of near-infrared, the optical sensing element includes two area's sensitive fluorescent part of near-infrared, when work, near-infrared two
The fluorescein on area light source activation measurand eyeground, two area's sensitive fluorescent part of near-infrared receive measurand fundus fluorescence element and generate
Two area's fluorescence of near-infrared and be imaged.
The light source is the light source of changeable near-infrared two area's light source mode and white light source mode.
It further include Mandibular supporting device, the Mandibular supporting device is oppositely arranged with fundus imaging apparatus.
The Mandibular supporting device includes chin rest and the label for being placed in chin rest, and the label is close for reflecting
Infrared 2nd area light.
The fundus imaging apparatus further includes optical positioning sensors, the optical positioning sensors and fundus camera position
In same plane, the optical positioning sensors are oppositely arranged with the label for being placed in chin rest, the optical positioning sensors
Receive two area's light of near-infrared of label reflection.
The fundus fluorescence imaging system further includes controller, the controller respectively with optical positioning sensors and eye
Bottom camera connection, the controller according to the received infrared 2nd areas optical oomputing measurand binocular visual axis of optical positioning sensors with
Deviation between the fundus camera optical axis receives instruction control fundus camera shooting eye fundus image and analyzes the clear of eye fundus image
Degree.
The fundus fluorescence imaging system further includes voice module, and the voice module is connect with controller, institute's predicate
Sound module is according to the clarity of deviation and image between the obtained measurand binocular visual axis of controller and the fundus camera optical axis
It issues measurand pupil position and adjusts prompt tone.
The fundus camera further includes fixation mark, and fixation target light source is that red of the wavelength in 700nm-800nm is visible
Point light source, the fixation mark is for assisting guidance measurand pupil to be aligned with the optical axial of fundus camera.
The wave-length coverage of two area's light of near-infrared is 1000nm-1700nm.
Two area's sensitive fluorescent part of near-infrared is InGaAs or HgCdTe.
A kind of fundus fluorescence imaging method, fundus camera obtain eyeground by shooting the measurand eyeground containing fluorescein
Two area's fluoroscopic visualization image of near-infrared.
Compared with prior art, the invention has the following advantages that
(1) have the function of bis- area's fluorescence imaging of Jin Hong, improve the resolution ratio of optical fundus blood vessel camera shooting.
(2) the bis- area's optical source wavelength range of Jin Hong used is 1000nm-1700nm, scatter attenuation when penetrating tissue
It is small.
(3) optical detector for experiencing two area's fluorescence of near-infrared has narrow band gap using InGaAs or HgCdTe,
It can get higher quantum efficiency.
(4) fluorescein has fabulous water solubility and biocompatibility, and quantum yield with higher, clear to generate
Clear image.
(5) fundus camera is equipped with outer illumination fixation mark, can assist the optical axial direction of pupil alignment fundus camera.
(6) it is equipped with label on bracket, binocular visual axis can be obtained in conjunction with optical positioning sensors.
(7) it is equipped with voice module, voice prompting adjustment measurand face direction and measurand pupil can be carried out to eye
The distance of bottom camera.
(8) light source is the light source of changeable Jin Hong bis- area's light source mode and white light source mode, and bis- area's light of Jin Hong is for fixed
Position and fluorescence imaging, white light are imaged for color fundus, and easy switching is easily operated.
Detailed description of the invention
Fig. 1 is the structural diagram of the present invention；
Fig. 2 is two area's fluoroscopic visualization image of eyeground near-infrared；
Fig. 3 is white light source eye fundus image；
Appended drawing reference:
1 is fundus imaging apparatus；2 be optical positioning sensors；3 be Mandibular supporting device；4 be fundus camera；5 be voice
Module；6 be measurand pupil；7 be pedestal；8 be fixation mark.
Specific embodiment
The present invention is described in detail with specific embodiment below in conjunction with the accompanying drawings.The present embodiment is with technical solution of the present invention
Premised on implemented, the detailed implementation method and specific operation process are given, but protection scope of the present invention is not limited to
Following embodiments.
Embodiment
A kind of fundus fluorescence imaging system, including fundus imaging apparatus 1, fundus imaging apparatus 1 include fundus camera 4, eye
Bottom camera 4 includes light source, optical sensing element and camera lens, and light source and camera lens are coaxial, and the operating mode of light source includes near-infrared two
Area's light source mode, optical sensing element include two area's sensitive fluorescent part of near-infrared, and when work, two area's light source activation of near-infrared is tested
The fluorescein on object eyeground, it is glimmering that two area's sensitive fluorescent part of near-infrared receives 2nd area of near-infrared that measurand fundus fluorescence element generates
Light is simultaneously imaged.
Fundus fluorescence imaging system further includes Mandibular supporting device 3, and Mandibular supporting device 3 and fundus imaging apparatus 1 are opposite
Setting.
Mandibular supporting device 3 includes chin rest and the label for being placed in chin rest, is marked for reflecting 2nd area of near-infrared
Light.
Fundus imaging apparatus 1 further includes optical positioning sensors 2, and optical positioning sensors 2 are located at same with fundus camera 4
Plane, optical positioning sensors 2 are oppositely arranged with the label for being placed in chin rest, and optical positioning sensors 2 receive label reflection
Two area's light of near-infrared.
Fundus fluorescence imaging system further includes controller, and controller connects with optical positioning sensors 2 and fundus camera 4 respectively
It connects, controller is regarded according to the received infrared 2nd areas optical oomputing measurand binocular visual axis of optical positioning sensors 2 and fundus camera 4
Deviation between axis receives instruction control fundus camera 4 and shoots eye fundus image and analyze the clarity of eye fundus image.
Fundus fluorescence imaging system further includes voice module 5, and voice module 5 is connect with controller, and voice module 5 is according to control
The clarity of deviation and image between 4 optical axis of the obtained measurand binocular visual axis of device processed and fundus camera issues measurand
6 position regulation prompting sound of pupil.
Fundus camera 4 further includes fixation mark 8, and the light source of fixation mark 8 is red visible point light of the wavelength in 700nm-800nm
Source, fixation mark is for assisting guidance measurand pupil 6 to be aligned with the optical axial of fundus camera 4.
The wave-length coverage of two area's light of near-infrared is 1000nm-1700nm.
Two area's sensitive fluorescent part of near-infrared is InGaAs or HgCdTe.
A kind of fundus fluorescence imaging method using fundus fluorescence imaging system, method includes the following steps:
Step S1: fluorescein enters eyeground；
Step S2: fundus camera 4 shoots fundus fluorescein angiography image.
The present embodiment proposes the system and method for 2nd area of near-infrared (NIR-II) fluorescence imaging, the excitation wavelength position of use
In the second biological organism optical window (1000nm~1700nm), light can be significantly reduced to penetrate the scattering in biological tissue existing
As in addition photon is absorbed by tissue less, autofluorescence effect reduction, the living body that can obtain high-resolution eyeground deep tissue is glimmering
Light imaging.
Light source is the light source of changeable near-infrared two area's light source mode and white light source mode.
The fluorescein of optical fundus blood vessel is flowed through by two area's light source activation of near-infrared, and passes through two area's sensitive fluorescent part of near-infrared
Detection excitation generate fluorescence (infrared 2nd areas sensitive fluorescent part be refer to rapidly detect with certain spatial resolution it is glimmering
The fluorescence that light element issues after being stimulated), obtain optical fundus blood vessel and deep tissue clearly image.Compared with prior art, this reality
Applying example may be implemented the high-resolution fast imaging of eyeground deep tissue, enhance the effect of fluorescence imaging method.
Fundus camera 4 is able to carry out conventional focusing, exposure and camera shooting movement after receiving controller instruction.
Mandibular supporting device 3 includes chin rest and the label that is placed on bracket, and chin rest is for measurand by lower jaw
It is placed on it, and forehead is close to upper bracket.
Fluorescein can arrive at optical fundus blood vessel tissue, vein through the circulatory system by injection device Safety Injection venous system
Injection device includes syringe and syringe needle, the requirement of fluorescein dosage and injection speed.
Near infrared light and common white light all emit along the boresight direction of fundus camera 4.
Fluorescein has safety, and water-soluble and biocompatibility can be transmitted, and have by the circulatory system
The quantum efficiency of effect does not have any toxicity to human body.
When using fundus fluorescence imaging system, comprising the following steps:
1) before injected fluorescein, patient medication allergies, confirmation fluorescein allergic reaction test are verified；
2) lower jaw is placed on ready chin rest by patient, and chin rest is close in jaw portion, passes through outer illumination fixation mark
Measurand pupil 6 is guided, and adjusts fundus camera 4, keeps the optical axis of camera optical system consistent with human eye's visual axis direction, by eye
The optical centre of bottom camera 4 is directed at patient s pupil center, and the distance of adjustment measurand pupil 6 to fundus camera 4 is until eyeground
Image clearly；
3) fluorescein is propelled through vein, dosage is determined according to patient's weight；
4) start timer while starting injected fluorescein, by the irradiation of nearly 2nd area infrared light, by fundus camera 4
The variation of fundus fluorescence is observed, different time, different parts is selected to shoot fluoroscopic visualization image；
5) it after carrying out fluoroscopic visualization, is shot using white-light illuminating and obtains conventional color fundus image；
Compared with prior art, the invention has the following advantages that
1) present invention increases bis- area's fluorescence imaging function of Jin Hong, improves on the basis of existing fundus angiography system
The resolution ratio of optical fundus blood vessel camera shooting；
2) bis- area's light source of Jin Hong that the present invention uses, scatter attenuation is small when penetrating tissue, can obtain high-resolution eye
The living body fluorescent of bottom deep tissue is imaged.
Fig. 1 is the present embodiment structural schematic diagram, and fundus imaging apparatus 1 and Mandibular supporting device 3 are slidably connected to pedestal 7
On.
Eyeground under near-infrared lighting condition is first carried out when shooting eye fundus image and takes the photograph piece (such as Fig. 2), and then quick closedown is closely red
Outer optical illumination starts white light source, shoots the eye fundus image (such as Fig. 3) under the conditions of white-light illuminating.
Claims (10)
1. a kind of fundus fluorescence imaging system, including fundus imaging apparatus (1), the fundus imaging apparatus (1) includes eyeground phase
Machine (4), the fundus camera (4) includes light source, optical sensing element and camera lens, and the light source and camera lens are coaxial, and feature exists
In the operating mode of the light source includes two area's light source mode of near-infrared, and the optical sensing element includes that 2nd area of near-infrared is glimmering
Photaesthesia part, when work, the fluorescein on two area light source activation measurand eyeground of near-infrared, two area's sensitive fluorescent part of near-infrared is connect
It receives two area's fluorescence of near-infrared that measurand fundus fluorescence element generates and is imaged.
2. a kind of fundus fluorescence imaging system according to claim 1, which is characterized in that further include Mandibular supporting device
(3), the Mandibular supporting device (3) is oppositely arranged with fundus imaging apparatus (1).
3. a kind of fundus fluorescence imaging system according to claim 2, which is characterized in that the Mandibular supporting device
It (3) include chin rest and the label for being placed in chin rest, the label is for reflecting two area's light of near-infrared.
4. a kind of fundus fluorescence imaging system according to claim 3, which is characterized in that the fundus imaging apparatus
It (1) further include optical positioning sensors (2), the optical positioning sensors (2) are generally aligned in the same plane with fundus camera (4), institute
It states optical positioning sensors (2) to be oppositely arranged with the label for being placed in chin rest, the optical positioning sensors (2) receive label
Two area's light of near-infrared of reflection.
5. a kind of fundus fluorescence imaging system according to claim 4, which is characterized in that system is imaged in the fundus fluorescence
System further includes controller, and the controller is connect with optical positioning sensors (2) and fundus camera (4) respectively, the controller
According to the received infrared 2nd areas optical oomputing measurand binocular visual axis of optical positioning sensors (2) and fundus camera (4) optical axis it
Between deviation, receive instruction control fundus camera (4) shooting eye fundus image and analyze the clarity of eye fundus image.
6. a kind of fundus fluorescence imaging system according to claim 5, which is characterized in that system is imaged in the fundus fluorescence
System further includes voice module (5), and the voice module (5) connect with controller, and the voice module (5) obtains according to controller
Measurand binocular visual axis and fundus camera (4) optical axis between deviation and image clarity issue measurand pupil
(6) position regulation prompting sound.
7. a kind of fundus fluorescence imaging system according to claim 1, which is characterized in that the fundus camera (4) is also
Including fixation mark (8), the light source of the fixation mark (8) is red visible point light source of the wavelength in 700nm-800nm, for assisting
Guidance measurand pupil (6) is aligned with the optical axial of fundus camera (4).
8. a kind of fundus fluorescence imaging system according to claim 1, which is characterized in that two area's light of near-infrared
Wave-length coverage is 1000nm-1700nm.
9. a kind of fundus fluorescence imaging system according to claim 1, which is characterized in that two area's fluorescence of near-infrared
Position-sensing unit is InGaAs or HgCdTe.
10. a kind of fundus fluorescence imaging method, which is characterized in that fundus camera (4) is by shooting tested pair containing fluorescein
As eyeground obtains two area's fluoroscopic visualization image of eyeground near-infrared.
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| CN106841155A (en) * | 2017-04-14 | 2017-06-13 | 苏州影睿光学科技有限公司 | A kind of wide spectrum fluorescence imaging device |
| CN107411707A (en) * | 2017-05-08 | 2017-12-01 | 武汉大学 | A kind of tumor-microvessel imager and tumor-microvessel imaging method |
| CN107811706A (en) * | 2017-11-27 | 2018-03-20 | 东北大学 | A kind of operation guiding system based on image transmission optical fibre |
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101254092A (en) * | 2007-03-02 | 2008-09-03 | 佳能株式会社 | Ophthalmologic photographing apparatus |
| CN104757933A (en) * | 2015-04-17 | 2015-07-08 | 宋金岩 | Handheld type fundus camera |
| CN106821303A (en) * | 2017-04-10 | 2017-06-13 | 上海健康医学院 | A kind of autonomous type Fundus photography imaging system and method |
| CN106841155A (en) * | 2017-04-14 | 2017-06-13 | 苏州影睿光学科技有限公司 | A kind of wide spectrum fluorescence imaging device |
| CN107411707A (en) * | 2017-05-08 | 2017-12-01 | 武汉大学 | A kind of tumor-microvessel imager and tumor-microvessel imaging method |
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Application publication date: 20190927 |