A kind of piezoelectric supersonic microinjection device of many types of syringe needle of adaptation of modularized design
Technical field
The present invention relates to the technical fields of biomedical relevant surgical device, more particularly to a kind of modularized design
It is adapted to the piezoelectric supersonic microinjection device of many types of syringe needle.
Background technique
With the fast development of modern biomedical, the application of biological cell microinjection is also more burning hot, such as transgenosis
Biology, human assistance reproduction, bio-pharmaceuticals and clone technology etc..Microinjection is divided into three processes: rupture of membranes is injected and is moved back
Needle.During rupture of membranes, since the object to be operated (eg. egg cell) is a fragile viscoelastic body, injection needle is inserted directly into
Method can make cell that huge deformation occur, be easy to cause the damage of cytoskeleton and internal structure, cause to be injected cell
Survival rate is low.Scientific research circle is directed to when microinjection how to reduce injury of the rupture of membranes in the process to cell, reduces injection vibration to thin
Born of the same parents' bring influences, and makes a large amount of research.Scientists introduce ultrasonic piezo technology, during rupture of membranes, injection needle needle
Point generate ultrasonic vibration, cell only need a very little deformation can smooth rupture of membranes, reduce in needle point insertion process to thin
It is damaged caused by born of the same parents.Oscillation crosswise of the vibration of needle point comprising axial vibration and other directions along vibration axis direction, it is excessive
Oscillation crosswise can increase the damage to cell.Therefore, how to inhibit oscillation crosswise is to further increase injection success rate and injection
The key of cell survival rate afterwards.As shown in Fig. 1 (a), ultrasonic vibration generator designed by Connecticut, USA university, the device
By piezoelectric ceramics, injection mironeedle and micropin fixed structure composition;The scheme of this piezoelectric ceramics postposition, so that energy is transmitted to
Injection needle distance is longer, and the vibration transmission of long range can generate harmful oscillation crosswise, be easy to cause to damage to cell.Such as figure
Shown in 1 (b), for the problems of ultrasonic vibration generator designed by University of Connecticut, University Of Suzhou Huang Haibo religion
The design scheme for proposing that piezoelectric ceramics is preposition is awarded, the program improves energy by reducing the distance between piezoelectric ceramics and injection needle
Transmission efficiency is measured, the unnecessary oscillation crosswise generated in vibration long range transmittance process is eliminated, reduces the damage to cell.
Nowadays, the preposition scheme of piezoelectric ceramics is widely used, the commercial PiezoXpert pressure produced such as Eppendorf company
Piezoelectric ceramics is directly connected with the metallic rod of fixed micropin by electric rupture of membranes instrument, and capacity usage ratio is high, can effectively carry out to cell
Film drilling.
At the same time, in microinjection field, common injection needle includes staight needle and looper two types.In microinjection
In the process, it is necessary first to fill the solution that DNA, sperm etc. are injected to injection needle.Since injection needle bore is superfine,
It is very time-consuming that enough substances are drawn using direct absorption method.In order to accelerate experiment process, it will usually be injected solution and load on
Injection needle tail end, by siphonage, solution slowly reaches injection needle tip, and siphonage compares direct completion method, greatly
Reduce the loading time greatly.Staight needle can use siphonage quick load and largely inject solution, therefore be suitable for transgenosis work
Journey, and staight needle is cheap, it is easy to make, it can be prepared in general laboratory, but staight needle is unfavorable under the microscope
Focusing, it is cumbersome.Looper is not available siphonage and is loaded tank solution due to special construction, therefore looper is only applicable to only
The scene of micro-injection solution, such as ICSI need to be drawn, and looper makes relative difficulty, needs special equipment, price is slightly expensive;
But looper is convenient to focus under micro-vision, in rupture of membranes, injection is in same focal plane with cell always during the withdraw of the needle,
It is easy to operate.
Although the prior art has been directed to microinjection device and has done many improvement, microscope equipment in the prior art
Be designed just for a kind of injection needle (staight needle or looper), and the field that different injection needle (staight needle or looper) is applicable in
Range is different, therefore, if needing to replace in entire microinjection device when carrying out different experiments Microinjections, increases
Add cost and increases the difficulty used.Further, microinjection device design in the prior art excessively complexity and one
Body, not only processing and assembly difficulty are big, processing cost is high, and since the service life of piezoelectric ceramics is limited, after being also unfavorable for
Phase maintenance.
Therefore, in view of the above technical problems, it is necessary to which different injection needles and design can be selected according to application scenarios by providing
Simply, be conducive to a kind of piezoelectric supersonic microinjection device of many types of syringe needle of adaptation of modularized design of later maintenance.
Summary of the invention
In view of this, being designed to provide for the embodiment of the present invention a kind of select different injection needles according to application scenarios and sets
Count a kind of piezoelectric supersonic microinjection device of many types of syringe needle of adaptation of modularized design that is simple, being conducive to later maintenance.This
Microinjection device provided by inventive embodiments based on modularized design is using replaceable injection pedestal design, so that micro-
The compatible different types of injection needle of injection device can select the injection needle of suitable type according to scene.Further, of the invention
Piezoelectric driving module and corresponding shell of the microinjection device using encapsulation provided by embodiment based on modularized design
Module effectively improves the anti-interference ability of microscope equipment and the overall weight of alleviator and is conducive to dissipating for piezoelectric ceramics
Heat.
To achieve the goals above, the technical solution that one embodiment of the invention provides is as follows: a kind of modularized design is fitted
Piezoelectric supersonic microinjection device with many types of syringe needle, comprising: connecting rod, for playing the extension rod of connection function;Piezoelectric Driving
Module is fixedly connected with the connecting rod, for providing the driving source of movement;Shell is connect with the connecting rod, is used for institute
It states piezoelectric driving module and accommodating space is provided;Compliant mechanism is connect with the piezoelectric driving module, is driven for transmitting the piezoelectricity
The movement that dynamic model block generates;Multiple base modules are alternatively connect with the compliant mechanism, and different pedestals is adapted to inhomogeneity
The injection needle of type and for fixing and seal the injection needle.
As a further improvement of the present invention, the multiple base module includes staight needle pedestal and looper pedestal.
As a further improvement of the present invention, the staight needle pedestal includes: staight needle injection needle, the needle section of the injection needle
It is linear;First injection head, including the interconnecting piece being connect with the compliant mechanism and for sealing the staight needle injection needle
First sealing ring;Second injection head is connect with first injection head, close including second for sealing the staight needle injection needle
Seal;Third injection head is connect with second injection head, for covering the staight needle pedestal;The staight needle injection needle passes through
The injection needle passageway formed by first injection head, the second injection head and third injection head.
As a further improvement of the present invention, the looper pedestal includes: looper injection needle, the needle section of the injection needle
For bending-like;First injection head, including the interconnecting piece being connect with the compliant mechanism and for sealing the looper injection needle
First sealing ring;Second injection head is connect with first injection head, close including second for sealing the looper injection needle
Seal;Third injection head is connect with second injection head, for covering the looper pedestal;The looper injection needle passes through
The injection needle passageway formed by first injection head, the second injection head and third injection head.
As a further improvement of the present invention, the connection type of the interconnecting piece of the compliant mechanism and first injection head
To be threadedly coupled.
As a further improvement of the present invention, the junction of the compliant mechanism and the base module is equipped with the second elasticity
Part.
As a further improvement of the present invention, the junction of the compliant mechanism and the piezoelectric driving module is provided with the
One elastic component.
As a further improvement of the present invention, the compliant mechanism uses one group of four columnar combined type triangle flexible hinge
Chain mechanism.
As a further improvement of the present invention, each of described Composite Triangular shape flexure hinge mechanism triangle is flexible
Linkage includes the first reed, the second reed and moving mass.
As a further improvement of the present invention, the angle of first reed and second reed is 90 degree.
The invention has the following advantages that
Microinjection device based on modularized design provided by the embodiment of the present invention uses packaged Piezoelectric Driving
Module, exposed piezoelectric ceramics compared with prior art, has better anti-interference ability and stability is higher.Further,
Microinjection device based on modularized design provided by the embodiment of the present invention uses one group of four columnar combined type triangle
Flexure hinge mechanism can not only inhibit the oscillation crosswise in injection process well, also reduce the processing of multiple groups flexible hinge
Difficulty and processing cost.Further, the microinjection device provided by the embodiment of the present invention based on modularized design uses
Interchangeable base module, so that the same microinjection device can select the base of different type injection needle according to use occasion
Seat module, has effectively widened the application range of microinjection device and has reduced experimentation cost, improves microinjection device
Later maintenance.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
The some embodiments recorded in invention, for those of ordinary skill in the art, without creative efforts,
It is also possible to obtain other drawings based on these drawings.
Fig. 1 (a) is the injection device structural schematic diagram of prior art piezoelectric ceramics postposition scheme;
Fig. 1 (b) is the injection device structural schematic diagram of the preposition scheme of prior art piezoelectric ceramics;
Fig. 2 is the structural schematic diagram (partially transparent figure and partial sectional view) of the microinjection device equipped with looper pedestal;
Fig. 3 is the schematic perspective view of compliant mechanism in Fig. 2;
Fig. 4 (a) is the main view of compliant mechanism in embodiment illustrated in fig. 3;
Fig. 4 (b) is the left view of compliant mechanism in embodiment illustrated in fig. 3;
Fig. 4 (c) is the top view of compliant mechanism in embodiment illustrated in fig. 3;
Fig. 4 (d) is the 3 dimensional drawing of compliant mechanism in embodiment illustrated in fig. 3;
Fig. 5 is the structural schematic diagram of the microinjection device equipped with staight needle pedestal;
Fig. 6 is the structural schematic diagram of the microinjection device equipped with looper pedestal;
Fig. 7 is the unassembled state that the microinjection device based on modularized design includes staight needle pedestal and looper pedestal
Decomposition diagram.
Description of symbols in attached drawing:
100, injection device 1, connecting rod 2, shell
3, piezoelectric driving module 4, compliant mechanism 5, base module
6, the first injection head 7, the second injection head 8, third injection head
10, injection needle 101 ', staight needle needle section 101, looper needle section
21, the first elastic component 23, the second elastic component 51, the first sealing ring
53, the second sealing ring 41, the first reed 42, the second reed
43, moving mass 61, interconnecting piece
Specific embodiment
Technical solution in order to enable those skilled in the art to better understand the present invention, below in conjunction with of the invention real
The attached drawing in example is applied, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's every other embodiment obtained without making creative work, all should belong to protection of the present invention
Range.
Join shown in Fig. 2, first embodiment of the invention provides a kind of piezoelectric supersonic of many types of syringe needle of adaptation of modularized design
Microinjection device 100.For the ease of showing the internal structure of microinjection device 100, microinjection device in Fig. 2
100 are shown in relation to base module 5 using transparent view, except the other parts of base module 5 are shown using cross-sectional view.A kind of module
Changing the piezoelectric supersonic microinjection device 100 for being adapted to many types of syringe needle designed includes: the connecting rod 1 for playing connection function, with
Connecting rod 1 is fixedly connected and the piezoelectric driving module 3 of the driving source for providing movement, connect and is used for pressure with connecting rod 1
Electric drive module 3 provides the shell 2 of accommodating space, connect with piezoelectric driving module and produces for transmitting the piezoelectric driving module
The compliant mechanism 4 of raw movement, the multiple base modules 5 alternatively being connect with the compliant mechanism 4.Different base modules
5 are adapted to different types of injection needle 10 and for fixing injection needle 10.
Referring to shown in Fig. 3 and Fig. 4 (a) to 4 (d), compliant mechanism 4 is columnar compound using one group four in the embodiment of the present invention
Formula triangle flexure hinge mechanism.Flexible hinge is a kind of peculair motion pair that displacement is generated using the flexible deformation of material, tool
Whether there is or not friction, gapless, easy to maintain, high resolution and can integration processing the advantages that.The classification of the flexible hinge on basis is main
There are four types of: straight beam type, straight round, ellipse and delta-type.Triangle (reed-type) flexible hinge makes up to a certain extent
Common gap formula hinge stroke short defect, and the hunt effect that this flexible hinge generates can lead in entire mechanism
Crossing reasonable structure design makes global error be reduced to minimum, the running precision for the flexible hinge that increased.Triangular hinge tool
Have that stroke is big, Static positioning accuracy is high, axis floats the excellent static characteristic such as small.It includes two triangles that the embodiment of the present invention, which uses,
The combined type triangular hinge that hinge is spliced.This combined type triangle not only has stroke big, Static positioning accuracy
High, axis floats the excellent static characteristic such as small, while increasing the stability of mechanism again, plays and leads to the axial vibration of injection needle
To effect, the precision of injection is improved.
In this embodiment, using one group of four columnar combined type triangle flexure hinge mechanism.It is compound in compliant mechanism 4
Each of triangle flexure hinge mechanism triangle flexure hinge mechanism includes the first reed 401, the second reed 402 and fortune
Motion block 403.Angle between first reed 401 and the second reed 402 is preferably 90 degree.The material of triangle compliant mechanism is not
Become rusty steel and elasticity modulus is more than or equal to 200Gpa.The embodiment of the present invention is only with one group of four columnar combined type triangle flexible hinge
Chain mechanism can not only inhibit the oscillation crosswise in injection process well, also reduce multiple groups flexible hinge processing difficulty and
Processing cost.
Referring to figure 5 and figure 6, the respectively structural schematic diagram and looper pedestal of the microinjection device equipped with staight needle pedestal
The structural schematic diagram of microinjection device.In this embodiment, multiple base modules include staight needle pedestal and looper pedestal.Staight needle
Pedestal and looper pedestal are alternatively connect with compliant mechanism 4 respectively, and microinjection device 100 can select straight according to application
Needle pedestal or looper pedestal.
As shown in figure 5, staight needle pedestal includes staight needle injection needle 10, the first injection head 6, the second injection head 7 and third injection
First 8, the first injection head 6, the second injection head 7 and third injection head 8 form an injection needle passageway so that staight needle injection needle 10 is worn
It crosses.As shown in fig. 6, the needle section 101 ' of staight needle injection needle 10 is linear.First injection head 6 includes connecting with compliant mechanism 4
Interconnecting piece (not shown in Fig. 5) and the first sealing ring 51 for sealing staight needle injection needle 10.Second injection head 7 and the first note
First 6 connection is penetrated, the second injection head 7 includes the second sealing ring 53 for sealing staight needle injection needle 10.Third injection head 8 and second
Injection head 7 connects, and third injection head 8 is the capping with central through hole, for covering staight needle pedestal 5.First sealing ring, 51 He
Second sealing ring 53 is specially 0 type sealing ring, and material can be using rubber, silica gel, elastic metallic etc..First sealing ring 51 and
On the one hand two sealing rings 53 play fixed function, on the other hand seal.
In the invention, the number of similar functions component in Fig. 6 is unified with Fig. 5 phase.As shown in fig. 6, looper pedestal packet
Include looper injection needle 10, the first injection head 6, the second injection head 7 and third injection head 8, the first injection head 6, the second injection head 7 and
Third injection head 8 forms an injection needle passageway so that looper injection needle 10 passes through.As shown in fig. 6, the needle of looper injection needle 10
Head 101 is bending-like.The range of the bending angle of needle section 101 is 25 degree to 30 degree.In this embodiment, bending angle is adopted
With 30 degree.First injection head 6 includes the interconnecting piece 61 (as shown in Figure 2) connecting with compliant mechanism 4 and injects for sealing looper
First sealing ring 51 of needle 10.Second injection head 7 is connect with the first injection head 6, and the second injection head 7 includes for sealing looper note
Penetrate the second sealing ring 53 of needle 10.Third injection head 8 is connect with the second injection head 7, and third injection head 8 is with central through hole
Capping, for covering staight needle pedestal 5.First sealing ring 51 and the second sealing ring 53 are specially O-ring seal, and material can use
Rubber, silica gel, elastic metallic etc..On the one hand first sealing ring 51 and the second sealing ring 53 play fixed function, on the other hand rise close
Envelope effect.
As shown in fig. 7, the microinjection device 100 based on modularized design include multiple and different base modules 5 without
Decomposition diagram with state.Multiple and different base modules 5 is respectively staight needle pedestal shown in fig. 5 and looper shown in fig. 6
Pedestal.Microinjection device 100 based on modularized design uses interchangeable base module 5, so that the same microinjection
Device can select the base module 5 of different type injection needle according to use occasion, effectively widen microinjection device
Application range and the later maintenance for reducing experimentation cost, improving microinjection device.When selecting different base modules,
The other parts of microinjection device 100 can share, and be effectively reduced cost.
In this embodiment, compliant mechanism 4 is to be threadedly coupled with the connection type of the interconnecting piece 61 of the first injection head 6.For
The junction of elimination thread pitch and increased unstability, compliant mechanism 4 and base module 5 is equipped with the second elastic component 23.
Second elastic component 23 can specifically use spring washer.Elastic washer is not solved only effectively by flexible deformation and locking effect
The instability problem certainly introduced due to thread pitch is also improved the effect for inhibiting oscillation crosswise, can preferably fastened
Microinjection device 100.
In this embodiment, piezoelectric driving module 3 is packaged piezoelectric ceramics, compared to the prior art exposed piezoelectricity
Ceramics, have better anti-interference ability and stability is higher.Connection type between piezoelectric driving module 3 and compliant mechanism 4
To be threadedly coupled.In order to eliminate thread pitch and increased unstability, the junction of compliant mechanism 4 and piezoelectric driving module 3
It is provided with the first elastic component 21.First elastic component 21 can specifically use spring washer.Elastic washer passes through flexible deformation and lock
It is set for using, not only efficiently solves the instability problem introduced due to thread pitch, also improve and inhibit oscillation crosswise
Effect can preferably fasten microinjection device 100.
Shell 2 is formed with accommodating space, and piezoelectric driving module 3 is placed in the accommodating space.In this embodiment, shell 2
It is not direct to be connected with piezoelectric driving module 3.Shown in referring to figure 5 and figure 6, shell 2 rejects part unnecessary portion (i.e. cylinder
The part of upper hollow out), to mitigate the overall weight of microinjection device 100, and be conducive to piezoelectric driving module 3 heat dissipation and
Increase the service life of piezoelectric driving module 3.
Injection device 100 provided by the embodiment of the present invention is ined succession a piezoelectric driving module 3 on connecting rod 1;Pressure
Electric drive module 3 is connected directly with compliant mechanism 4, passes motion to compliant mechanism 4;Movement by the filtering of compliant mechanism 4,
Balance passes to the base module 5 of front;For the ease of being applicable in different scenes, base module 5 has multiple and alternatively
It is connected with compliant mechanism 4.
Microinjection device based on modularized design provided by the embodiment of the present invention uses packaged Piezoelectric Driving
Module, exposed piezoelectric ceramics compared with prior art, has better anti-interference ability and stability is higher.
Microinjection device based on modularized design provided by the embodiment of the present invention is columnar compound using one group four
Formula triangle flexure hinge mechanism can not only inhibit the oscillation crosswise in injection process well, also reduce multiple groups flexible hinge
The difficulty and processing cost of chain processing.
Microinjection device based on modularized design provided by the embodiment of the present invention uses interchangeable base module,
So that the same microinjection device can select the base module of different type injection needle according to use occasion, effectively widen
The application range of microinjection device and the later maintenance for reducing experimentation cost, improving microinjection device
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case where without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Benefit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent elements of the claims
Variation is included within the present invention.Any reference signs in the claims should not be construed as limiting the involved claims.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.