CN109984279A - Yellow croaker ecologic breeding feed - Google Patents
Yellow croaker ecologic breeding feed Download PDFInfo
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- CN109984279A CN109984279A CN201910229522.1A CN201910229522A CN109984279A CN 109984279 A CN109984279 A CN 109984279A CN 201910229522 A CN201910229522 A CN 201910229522A CN 109984279 A CN109984279 A CN 109984279A
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- antibacterial peptide
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- ramee
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- 241001596950 Larimichthys crocea Species 0.000 title claims abstract description 36
- 238000009395 breeding Methods 0.000 title claims abstract description 19
- 230000001488 breeding effect Effects 0.000 title claims abstract description 19
- 239000003910 polypeptide antibiotic agent Substances 0.000 claims abstract description 47
- 244000050510 Cunninghamia lanceolata Species 0.000 claims abstract description 24
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000004202 carbamide Substances 0.000 claims abstract description 22
- SOQBVABWOPYFQZ-UHFFFAOYSA-N oxygen(2-);titanium(4+) Chemical compound [O-2].[O-2].[Ti+4] SOQBVABWOPYFQZ-UHFFFAOYSA-N 0.000 claims abstract description 17
- 238000002360 preparation method Methods 0.000 claims abstract description 17
- 235000013312 flour Nutrition 0.000 claims abstract description 16
- 239000007788 liquid Substances 0.000 claims abstract description 16
- 239000000843 powder Substances 0.000 claims abstract description 16
- 239000002985 plastic film Substances 0.000 claims abstract description 15
- 229920006255 plastic film Polymers 0.000 claims abstract description 15
- 239000006041 probiotic Substances 0.000 claims abstract description 15
- 235000018291 probiotics Nutrition 0.000 claims abstract description 15
- 239000004575 stone Substances 0.000 claims abstract description 10
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 8
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 8
- 241000286209 Phasianidae Species 0.000 claims abstract description 8
- 240000006394 Sorghum bicolor Species 0.000 claims abstract description 8
- 235000011684 Sorghum saccharatum Nutrition 0.000 claims abstract description 8
- 240000008042 Zea mays Species 0.000 claims abstract description 8
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 8
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 8
- 210000000988 bone and bone Anatomy 0.000 claims abstract description 8
- 235000005822 corn Nutrition 0.000 claims abstract description 8
- 229910052500 inorganic mineral Inorganic materials 0.000 claims abstract description 8
- 239000011707 mineral Substances 0.000 claims abstract description 8
- 235000017060 Arachis glabrata Nutrition 0.000 claims abstract description 7
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- 235000012054 meals Nutrition 0.000 claims abstract description 7
- 235000020232 peanut Nutrition 0.000 claims abstract description 7
- 239000011833 salt mixture Substances 0.000 claims abstract description 7
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 230000003213 activating effect Effects 0.000 claims description 12
- 239000007822 coupling agent Substances 0.000 claims description 12
- 229910052719 titanium Inorganic materials 0.000 claims description 11
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- 239000002994 raw material Substances 0.000 claims description 7
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims description 6
- 230000004913 activation Effects 0.000 claims description 6
- 238000001354 calcination Methods 0.000 claims description 6
- 239000001768 carboxy methyl cellulose Substances 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
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- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 3
- 102000013585 Bombesin Human genes 0.000 claims description 3
- 108010051479 Bombesin Proteins 0.000 claims description 3
- DNDCVAGJPBKION-DOPDSADYSA-N bombesin Chemical group C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(N)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](CC=1NC2=CC=CC=C2C=1)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H]1NC(=O)CC1)C(C)C)C1=CN=CN1 DNDCVAGJPBKION-DOPDSADYSA-N 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 2
- 150000001875 compounds Chemical class 0.000 claims description 2
- 244000131522 Citrus pyriformis Species 0.000 claims 1
- 241000726221 Gemma Species 0.000 claims 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 11
- 201000010099 disease Diseases 0.000 abstract description 9
- 230000004083 survival effect Effects 0.000 abstract description 4
- 230000002180 anti-stress Effects 0.000 abstract description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 15
- 239000000243 solution Substances 0.000 description 15
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 11
- 241000251468 Actinopterygii Species 0.000 description 7
- 239000000463 material Substances 0.000 description 7
- 241000194107 Bacillus megaterium Species 0.000 description 6
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- 238000012545 processing Methods 0.000 description 6
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- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 5
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- 241001417494 Sciaenidae Species 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
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- 210000000952 spleen Anatomy 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/143—Fermentum
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Physiology (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Inorganic Chemistry (AREA)
- Insects & Arthropods (AREA)
- Marine Sciences & Fisheries (AREA)
- Birds (AREA)
- Biochemistry (AREA)
- Fodder In General (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention proposes a kind of yellow croaker ecologic breeding feeds, according to the number of parts by weight, including following components: 50~60 parts of corn flour, 14~18 parts of sorghum flour, 20~30 parts of fermented bean dregs, 16~25 parts of pheasant bone powder, 16~25 parts of peanut meal, 10~13 parts of decomposed ramee, 1~3 part of decomposed China fir leaf, 14~18 parts of medical stone powder, 0.6~1.8 part of mineral salt mixture, 0.5~1.5 part of multidimensional premix, nano-titanium dioxide -4~9 parts of antibacterial peptide complexes and 3~6 parts of probiotics；Decomposed ramee the preparation method comprises the following steps: being drenched after ramee is smashed with urea liquid, covered plastic film decomposed 5 months, the preparation method of decomposed China fir leaf: being drenched after China fir leaf is smashed with urea liquid, covered plastic film decomposed 3 months.The Feed Energy improves the anti-stress ability of yellow croaker, reduces disease incidence, improves survival rate.
Description
Technical field
The invention belongs to technical field of fertilizers, and in particular to a kind of yellow croaker ecologic breeding feed.
Background technique
Yellow croaker is divided into big little yellow croaker also known as yellow croaker, belongs to net-rope, Sciaenidae.There are two hard stones in fish head
Head is Yellow Croaker Ear-stone, and it is also known as " sciaenoids ".Larimichthys crocea also known as big fresh, Cymbaria dahurica, sweet osmanthus yellow croaker；Little yellow croaker is also known as small fresh, little Huang
Flower, cuke fish；Big little yellow croaker and hairtail are collectively known as the big marine products in China three.
Yellow croaker protein rich in, microelement and vitamin have good benefiting action to human body, to constitution void
For the weak and middle-aged and the old, edible yellow croaker will receive good food therapy effect.Yellow croaker trace elements of selenium rich in can be removed
The free radical that body metabolism generates, can delay senescence, and have prophylactic-therapeutic effect to various cancers.Chinese medicine thinks that yellow croaker has invigorating the spleen liter
, there is good efficacy in the effect of stomach, tranquilizing the mind stop dysentery, QI invigorating replenishing essence to anaemia, insomnia, dizziness, loss of appetite and women's postpartum weakness.
Since transition is caught, Wild Rhubarb fish quantity sharply declines, and can not meet consumer demand, the nineties Larimichthys crocea
Artificial breeding be succeeded, with the continuous expansion of Larimichthys crocea aquaculture scale, disease also gradually increases, often occurs
Some diseases lead to the immunity degradation of Larimichthys crocea, the even dead phenomenon of Larimichthys crocea immunity degradation occur.Wherein Larimichthys crocea
Vibriosis, bacterial gill, enteritis disease disease incidence are higher, and easily infect rapidly in a relatively short period of time, seriously affect
The economic benefit of the Larimichthys crocea speed of growth and cultivation, constrains the sustainable development of rheum officinale fish culture.How Larimichthys crocea is improved
The immunity of body is effective against the invasion of disease, reduces morbidity and the death rate, becomes people and prevents and treats rheum officinale disease
Point of penetration.Research indicates that Chinese herbal medicine has the function of adjusting body health, enhancing immunity of organisms, and the kind of Chinese herbal medicine
Various, pharmacological property multiplicity is cheap, resourceful, not only helps raising body using Chinese herbal medicine production immunopotentiator and exempts from
Epidemic disease power, and since Chinese herbal medicine belongs to natural materials, pollution is few, meets the requirement of green fishery, is conducive to people and pacifies to food
Full requirement.
Summary of the invention
To solve the above-mentioned problems of the prior art, the present invention proposes a kind of yellow croaker ecologic breeding feed, the Feed Energy
The anti-stress ability for enough improving yellow croaker, reduces disease incidence, improves survival rate.
The technical scheme of the present invention is realized as follows:
A kind of yellow croaker ecologic breeding feed, according to the number of parts by weight, including following raw material:
50~60 parts of corn flour, 14~18 parts of sorghum flour, 20~30 parts of fermented bean dregs, 16~25 parts of pheasant bone powder, flower
Raw 16~25 parts of the dregs of rice, 10~13 parts of decomposed ramee, 1~3 part of decomposed China fir leaf, 14~18 parts of medical stone powder, mineral salt mixing
0.6~1.8 part of material, 0.5~1.5 part of multidimensional premix, nano-titanium dioxide -4~9 parts of antibacterial peptide complexes and probiotics 3
~6 parts；The decomposed ramee the preparation method comprises the following steps: drenched after ramee is smashed with urea liquid, covered plastic film
Decomposed 5 months, the preparation method of the decomposed China fir leaf: being drenched after China fir leaf is smashed with urea liquid, and covered plastic film is rotten
Ripe 3 months.
Preferably, the nano-titanium dioxide-antibacterial peptide complexes are prepared by the following method to obtain:
1) it by nano-titanium dioxide after 500~700 DEG C carry out 4~6h of calcining, is cooled to room temperature, is then added to activating solution
Middle progress ultrasonic disperse handles 6~8h, then the dry nano-titanium dioxide that can be obtained activation of filtration washing；
2) antibacterial peptide is added to the water to the antibacterial peptide solution for being configured to 10~20g/L, then adds suitable chitosan
Stirring and dissolving, is warming up to 60~70 DEG C, and suitable titanate coupling agent is then added, and keeps the temperature 3~6h, is being evaporated drying i.e.
It can.
Preferably, the activating solution is by the citric acid of 9~12g/L, the sodium carboxymethylcellulose of 7~11g/L, 10~15g/
L urea and deionized water form.
Preferably, the mass ratio of the chitosan and the antibacterial peptide is 2~3:1, the antibacterial peptide and the metatitanic acid
The mass ratio of ester coupling agent is 1:6~8.
Preferably, the antibacterial peptide is bombesin antibacterial peptide.
Preferably, the probiotics is the mixed of bacillus megaterium, lactobacillus fermenti, saccharomyces cerevisiae and bacillus subtilis
Combined bacteria.
The preparation method of yellow croaker ecologic breeding feed of the present invention, comprising the following steps:
1) it weighs after above-mentioned raw materials are crushed and is being mixed according to the proportion, obtain mixed material；
2) mixed material is moved into modulator, the water for being passed through 95~100 DEG C according to the 15~17% of mixed material quality steams
Controlled atmosphere system, then by modulated mixed material 95~100 DEG C at a temperature of enter extrusion granulator feed is made
Grain.
There are mainly two types of crystal habits for nano-titanium dioxide: anatase titanium dioxide (Anatase) and rutile-type (Rutile).Golden red
Stone-type titanium dioxide is more stable and fine and close than anatase titanium dioxide, there is higher hardness, density, dielectric constant and refractive index,
Covering power and tinting strength, tinting power are also higher.And anatase titanium dioxide is in the luminance factor rutile-type titanium dioxide of visible light shortwave part
Titanium is high, band blue color, and lower than rutile-type to the absorbability of ultraviolet light, and photocatalytic activity is higher than rutile-type.?
Under certain condition, anatase titanium dioxide can be converted into rutile titanium dioxide.
Antibacterial peptide former refers to that the one kind generated in insect bodies through induction has the basic polypeptide substance of antibacterial activity, molecular weight
2000~7000 or so, it is made of 20~60 amino acid residues.This kind of active peptides majority has strong basicity, thermal stability
And the features such as broad-spectrum antiseptic.First antibacterial peptide being found is 1980 by Sweden scientist G.Boman etc. in the world
People through injection cloaca through rod bacterium and Escherichia coli induction cherish guppy day silkworm chrysalis generation the polypeptide with antibacterial activity, name for
Cecropins。
Beneficial effects of the present invention:
Nano-titanium dioxide-antibacterial peptide complexes are added in feed of the invention, such compound is before the application
It is not reported, is not also reported for adding in fresh water fish feed.Nano-titanium dioxide is answered by antibacterial peptide
While conjunction increases its palatability, also enhances its fresh-water fishes body absorption and play its antibacterial enhancing immunocompetence, while
Enhance fresh-water fishes anti-stress ability.
Specific embodiment
Embodiment 1
A kind of yellow croaker ecologic breeding feed, according to the number of parts by weight, including following raw material:
56 parts of corn flour, 16 parts of sorghum flour, 23 parts of fermented bean dregs, 19 parts of pheasant bone powder, 20 parts of peanut meal, decomposed ramie
11 parts of fiber, 2 parts of decomposed China fir leaf, 16 parts of medical stone powder, 1.1 parts of mineral salt mixture, 1.3 parts of multidimensional premix, nano-silica
Change titanium -6 parts of antibacterial peptide complexes and 4 parts of probiotics；Decomposed ramee the preparation method comprises the following steps: being used after ramee is smashed
Urea liquid is drenched, and covered plastic film decomposed 5 months, the preparation method of decomposed China fir leaf: molten with urea after China fir leaf is smashed
Liquid is drenched, and covered plastic film decomposed 3 months.Probiotics is bacillus megaterium, lactobacillus fermenti, saccharomyces cerevisiae and withered grass
The Mixed Microbes of bacillus.
Nano-titanium dioxide-antibacterial peptide complexes are prepared by the following method to obtain:
1) it by nano-titanium dioxide after 600 DEG C carry out calcining 5h, is cooled to room temperature, is then added in activating solution and is surpassed
Sound decentralized processing 7h, then filtration washing drying can be obtained the nano-titanium dioxide of activation；
2) antibacterial peptide is added to the water to the antibacterial peptide solution for being configured to 16g/L, then adds suitable chitosan stirring
Dissolution, is warming up to 67 DEG C, and suitable titanate coupling agent is then added, and keeps the temperature 5h, dry being evaporated.
Activating solution is made of the citric acid of 11g/L, the sodium carboxymethylcellulose of 9g/L, 12g/L urea and deionized water.
Preferably, the mass ratio of the chitosan and the antibacterial peptide is 2:1, the matter of antibacterial peptide and titanate coupling agent
The ratio between amount is 1:6.Antibacterial peptide is bombesin antibacterial peptide.
Embodiment 2
A kind of yellow croaker ecologic breeding feed, according to the number of parts by weight, including following raw material:
50 parts of corn flour, 14 parts of sorghum flour, 30 parts of fermented bean dregs, 16 parts of pheasant bone powder, 25 parts of peanut meal, decomposed ramie
10 parts of fiber, 1 part of decomposed China fir leaf, 14 parts of medical stone powder, 0.6 part of mineral salt mixture, 0.5 part of multidimensional premix, nano-silica
Change titanium -4 parts of antibacterial peptide complexes and 3 parts of probiotics；Decomposed ramee the preparation method comprises the following steps: being used after ramee is smashed
Urea liquid is drenched, and covered plastic film decomposed 5 months, the preparation method of decomposed China fir leaf: molten with urea after China fir leaf is smashed
Liquid is drenched, and covered plastic film decomposed 3 months.Probiotics is bacillus megaterium, lactobacillus fermenti, saccharomyces cerevisiae and withered grass
The Mixed Microbes of bacillus.
1) it by nano-titanium dioxide after 500 DEG C carry out calcining 6h, is cooled to room temperature, is then added in activating solution and is surpassed
Sound decentralized processing 6h, then filtration washing drying can be obtained the nano-titanium dioxide of activation；
2) antibacterial peptide is added to the water to the antibacterial peptide solution for being configured to 10g/L, then adds suitable chitosan stirring
Dissolution, is warming up to 60 DEG C, and suitable titanate coupling agent is then added, and keeps the temperature 6h, dry being evaporated.
Activating solution is made of the citric acid of 9g/L, the sodium carboxymethylcellulose of 11g/L, 10g/L urea and deionized water.Shell
The mass ratio of glycan and antibacterial peptide is 3:1, and the mass ratio of antibacterial peptide and titanate coupling agent is 1:7.Antibacterial peptide is frog skin
Plain antibacterial peptide.
Embodiment 3
A kind of yellow croaker ecologic breeding feed, according to the number of parts by weight, including following raw material:
53 parts of corn flour, 18 parts of sorghum flour, 21 parts of fermented bean dregs, 25 parts of pheasant bone powder, 16 parts of peanut meal, decomposed ramie
13 parts of fiber, 3 parts of decomposed China fir leaf, 15 parts of medical stone powder, 1.6 parts of mineral salt mixture, 1.5 parts of multidimensional premix, nano-silica
Change titanium -9 parts of antibacterial peptide complexes and 6 parts of probiotics；Decomposed ramee the preparation method comprises the following steps: being used after ramee is smashed
Urea liquid is drenched, and covered plastic film decomposed 5 months, the preparation method of decomposed China fir leaf: molten with urea after China fir leaf is smashed
Liquid is drenched, and covered plastic film decomposed 3 months.Probiotics is bacillus megaterium, lactobacillus fermenti, saccharomyces cerevisiae and withered grass
The Mixed Microbes of bacillus.
1) it by nano-titanium dioxide after 700 DEG C carry out calcining 4h, is cooled to room temperature, is then added in activating solution and is surpassed
Sound decentralized processing 8h, then filtration washing drying can be obtained the nano-titanium dioxide of activation；
2) antibacterial peptide is added to the water to the antibacterial peptide solution for being configured to 20g/L, then adds suitable chitosan stirring
Dissolution, is warming up to 70 DEG C, and suitable titanate coupling agent is then added, and keeps the temperature 3h, dry being evaporated.
Activating solution is made of the citric acid of 12g/L, the sodium carboxymethylcellulose of 7g/L, 13g/L urea and deionized water.Shell
The mass ratio of glycan and antibacterial peptide is 2:1, and the mass ratio of antibacterial peptide and titanate coupling agent is 1:8.
Embodiment 4
A kind of yellow croaker ecologic breeding feed, according to the number of parts by weight, including following raw material:
60 parts of corn flour, 16 parts of sorghum flour, 20 parts of fermented bean dregs, 18 parts of pheasant bone powder, 23 parts of peanut meal, decomposed ramie
12 parts of fiber, 2 parts of decomposed China fir leaf, 16 parts of medical stone powder, 1.8 parts of mineral salt mixture, 1.1 parts of multidimensional premix, nano-silica
Change titanium -7 parts of antibacterial peptide complexes and 5 parts of probiotics；Decomposed ramee the preparation method comprises the following steps: being used after ramee is smashed
Urea liquid is drenched, and covered plastic film decomposed 5 months, the preparation method of decomposed China fir leaf: molten with urea after China fir leaf is smashed
Liquid is drenched, and covered plastic film decomposed 3 months.Probiotics is bacillus megaterium, lactobacillus fermenti, saccharomyces cerevisiae and withered grass
The Mixed Microbes of bacillus.
Nano-titanium dioxide-antibacterial peptide complexes are prepared by the following method to obtain:
1) it by nano-titanium dioxide after 600 DEG C carry out calcining 6h, is cooled to room temperature, is then added in activating solution and is surpassed
Sound decentralized processing 6h, then filtration washing drying can be obtained the nano-titanium dioxide of activation；
2) antibacterial peptide is added to the water to the antibacterial peptide solution for being configured to 18g/L, then adds suitable chitosan stirring
Dissolution, is warming up to 63 DEG C, and suitable titanate coupling agent is then added, and keeps the temperature 5h, dry being evaporated.
Activating solution is made of the citric acid of 11g/L, the sodium carboxymethylcellulose of 10g/L, 15g/L urea and deionized water.
The mass ratio of chitosan and antibacterial peptide is 3:1, and the mass ratio of the antibacterial peptide and the titanate coupling agent is 1:6.
Test example
With batch of, bouncing, the essentially identical Larimichthys crocea of size weight is cultivated for selection, sets control group, real
Apply a group.Embodiment group feeds the yellow croaker ecologic breeding feed of embodiment 1 to 4 respectively, and control group feeds control group feed, and totally 5
A processing, each processing in triplicate, are averaged.Guarantee that each bucket water quality is identical, every barrel is put in a suitable place to breed 100, test into
Row three months, disease incidence, survival rate are measured after experiment.
60 parts of corn flour, 16 parts of sorghum flour, 20 parts of fermented bean dregs, 18 parts of pheasant bone powder, 23 parts of peanut meal, decomposed ramie
12 parts of fiber, 2 parts of decomposed China fir leaf, 16 parts of medical stone powder, 1.8 parts of mineral salt mixture, 1.1 parts of multidimensional premix, nano-silica
Change 7 parts of titanium and 5 parts of probiotics；Decomposed ramee the preparation method comprises the following steps: drenched after ramee is smashed with urea liquid,
Covered plastic film decomposed 5 months, the preparation method of decomposed China fir leaf: being drenched after China fir leaf is smashed with urea liquid, covers modeling
Material film decomposed 3 months.Probiotics is the mixed of bacillus megaterium, lactobacillus fermenti, saccharomyces cerevisiae and bacillus subtilis
Combined bacteria.
Table 1
| Morbidity item number, item | Disease incidence, % | Survive item number, item | Survival rate, % | |
| Control group | 42 | 42 | 75 | 75 |
| Embodiment 1 | 13 | 13 | 98 | 98 |
| Embodiment 2 | 16 | 16 | 95 | 95 |
| Embodiment 3 | 11 | 11 | 97 | 97 |
| Embodiment 4 | 12 | 12 | 96 | 96 |
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (6)
1. a kind of yellow croaker ecologic breeding feed, which is characterized in that according to the number of parts by weight, including following raw material:
50~60 parts of corn flour, 14~18 parts of sorghum flour, 20~30 parts of fermented bean dregs, 16~25 parts of pheasant bone powder, peanut meal
16~25 parts, 10~13 parts of decomposed ramee, 1~3 part of decomposed China fir leaf, 14~18 parts of medical stone powder, mineral salt mixture 0.6
~1.8 parts, 0.5~1.5 part of multidimensional premix, nano-titanium dioxide -4~9 parts of antibacterial peptide complexes and 3~6 parts of probiotics；
The decomposed ramee the preparation method comprises the following steps: drenched after ramee is smashed with urea liquid, covered plastic film decomposed 5
Month, the preparation method of the decomposed China fir leaf: being drenched after China fir leaf is smashed with urea liquid, and covered plastic film decomposed 3 months
?.
2. yellow croaker ecologic breeding feed according to claim 1, which is characterized in that the nano-titanium dioxide-antibacterial peptide
Compound is prepared by the following method to obtain:
1) by nano-titanium dioxide after 500~700 DEG C carry out 4~6h of calcining, be cooled to room temperature, be then added in activating solution into
Row ultrasonic disperse handles 6~8h, then the dry nano-titanium dioxide that can be obtained activation of filtration washing；
2) antibacterial peptide is added to the water to the antibacterial peptide solution for being configured to 10~20g/L, then adds suitable chitosan stirring
Dissolution, is warming up to 60~70 DEG C, and suitable titanate coupling agent is then added, and keeps the temperature 3~6h, dry being evaporated.
3. yellow croaker ecologic breeding feed according to claim 2, which is characterized in that the activating solution by 9~12g/L lemon
Lemon acid, the sodium carboxymethylcellulose of 7~11g/L, 10~15g/L urea and deionized water form.
4. yellow croaker ecologic breeding feed according to claim 2, which is characterized in that the chitosan and the antibacterial peptide
Mass ratio is 2~3:1, and the mass ratio of the antibacterial peptide and the titanate coupling agent is 1:6~8.
5. yellow croaker ecologic breeding feed according to claim 2, which is characterized in that the antibacterial peptide is bombesin antibacterial
Peptide.
6. yellow croaker ecologic breeding feed according to claim 1, which is characterized in that the probiotics is huge gemma bar
Bacterium, lactobacillus fermenti, saccharomyces cerevisiae and bacillus subtilis Mixed Microbes.
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| CN112048084A (en) * | 2020-08-21 | 2020-12-08 | 浙江隆泰医疗科技股份有限公司 | Preparation method of low-temperature crosslinked polyvinyl alcohol composite antibacterial peptide material |
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| CN112048084A (en) * | 2020-08-21 | 2020-12-08 | 浙江隆泰医疗科技股份有限公司 | Preparation method of low-temperature crosslinked polyvinyl alcohol composite antibacterial peptide material |
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