CN109965136A - A kind of rejected layer meat improved formulations, preparation method and its application - Google Patents
A kind of rejected layer meat improved formulations, preparation method and its application Download PDFInfo
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- CN109965136A CN109965136A CN201910276731.1A CN201910276731A CN109965136A CN 109965136 A CN109965136 A CN 109965136A CN 201910276731 A CN201910276731 A CN 201910276731A CN 109965136 A CN109965136 A CN 109965136A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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Abstract
The invention belongs to feed additive field, it is related to a kind of rejected layer meat improved formulations and its preparation method and application.The meat improved formulations include Rhodotorula mucilaginose, S. cervisiae, bacillus subtilis, vitamin E, plants essential oil, preparation method is uniformly to mix each component, according to feed total weight with 0.1% ~ 0.3% additive amount, which is added in animal and fowl fodder.Improved formulations of the invention can be used as the substitute of antibiotic, eliminate medicament residue, improves laying hen meat egg product quality, significantly improves the content of inosinicacid and intramuscular fat in laying hen muscle, keep chicken cunning tender tasty and refreshing；It can also be used to prevent and treat laying hen diarrhea, improve immunity, premunition, reduce laying cycle of laying hens death and culling rate, and feed conversion rate and egg laying performance can be improved.It is also beneficial to purification environment of chicken house, protects and improves the ecological environment.
Description
Technical field
The present invention relates to a kind of rejected layer meat improved formulations, belong to feed additive field.
Background technique
Currently, the number of animals raised of commercial generation laying hen tends towards stability both at home and abroad, structure gradual perfection, scale laying hen number of fields are produced
Amount is stepped up, and scale laying hen breeding technology and equipment level improve, and is generally used standardization cage mode, is improved
Breeding layer chicken production efficiency.It is most of all to use highdensity cage but to pursue maximum economic benefit, laying hen can be made in this way
Be chronically at stress state and by many unfavorable factors stimulate, cause China's breeding layer chicken compared with European and American developed countries,
Laying hen occurs egg laying performance decline too early and is eliminated by poultry place.In fact, rejected layer meat have high protein, low fat,
The features such as low cholesterol, the content ratio of protein is compared with high, amino acid classes are more, digestibility is high in chicken, it is easy to by human body
It is absorbed and utilized, plays the role of enhancing physical strength, keep fit and healthy.But connective tissue content is higher in the muscle of this eliminate chicken, tenderness
It is poor, it is difficult to cook, how make this kind of muscle tenderization, to improve the edible quality of meat, this is imitated to the economic of laying hen production is improved
Beneficial important in inhibiting.
Summary of the invention
For overcome the deficiencies in the prior art, the purpose of the present invention is to provide a kind of rejected layer meat improved formulations,
Preparation method and its application can be used as the substitute of antibiotic, eliminate medicament residue, significantly improve inosinicacid in laying hen muscle
With the content of intramuscular fat, keeps chicken cunning tender tasty and refreshing, improves laying hen meat egg product quality,；It can also be used to prevent and treat laying hen diarrhea, mention
High immunity premunition, reduces laying cycle of laying hens death and culling rate, and feed conversion rate and egg laying performance can be improved.It is also beneficial to net
Change environment of chicken house, protects and improve the ecological environment.
The purpose of the present invention is what is be achieved through the following technical solutions:
Rejected layer meat improved formulations include: Rhodotorula mucilaginose, S. cervisiae, bacillus subtilis, plants essential oil
And vitamin E, the parts by weight of each component are as follows:
Rhodotorula mucilaginose: 20-60 parts, preferably 30-50 parts；
S. cervisiae: 3-30 parts, preferably 5-20 parts；
Bacillus subtilis: 5-40 parts, preferably 10-30 parts；
Plants essential oil: 5-40 parts, preferably 10-30 parts；
Vitamin E: 3-20 parts, preferably 5-10 parts.
The preparation method of above-mentioned rejected layer meat modifier is to be uniformly mixed above-mentioned each component, becomes its uniformity
Different coefficient CV obtains the meat modifier less than 5%.
Plants essential oil is fragrance type, is provided by Nanjing Ze Lang Biotechnology Co., Ltd, main component is plants essential oil
It (15%) mainly include 25% carvacrol, 25% Thymol with high-quality carrier.
The most handy dry powder formulations of the probiotics Rhodotorula mucilaginose, S. cervisiae, bacillus subtilis, and every kind of bacterial strain
Pure bacterium powder viable count should be not less than l~5 × 109cfu/g.S. cervisiae, bacillus subtilis most handy dry powder formulations (by
Nanjing Ze Lang Biotechnology Co., Ltd processing).
Rhodotorula mucilaginose, S. cervisiae, bacillus subtilis preparation method, include the following steps:
1) seed culture medium 500ml is prepared, 121 DEG C sterilize 15 minutes；
2) strain frozen accordingly is inoculated in seed culture medium, Rhodotorula mucilaginose and S. cervisiae culture 24 are small
When；
3) fermentation medium, seeding tank 100L are prepared, fermentor is 1 ton, and 121 DEG C sterilize 15 minutes；
4) 2) seed that culture obtain is added in seeding tank, is fermented about 18 hours；
5) tunning of seeding tank is squeezed into fermentor by air pressure, continues fermentation 10-12 hours.
6) bacterium mud after fermentation, is collected using plate-frame filtering bacterium solution, the carrier of identical weight is added, after low temperature drying i.e.
The Rhodotorula mucilaginose of system, S. cervisiae, bacillus subtilis.
Wherein the rhodotorula mucilaginosa and saccharomyces cerevisiae seed culture based formulas are yeast extract 1%, peptone 2%,
Glucose 2%.Fermentation medium is yeast extract 1%, peptone 2%, glucose 2%, GPE 0.1%.In fermentation process,
It is 5.5-6.5 by adding ammonium hydroxide to keep pH value, dissolved oxygen is 20% or so, mends sugar formula: glucose 20%, dipotassium hydrogen phosphate
0.16%, ammonium chloride 0.14%.
Wherein the bacillus subtilis seed culture medium is peptone 1%, yeast extract 0.5%, sodium chloride
1%, natural pH.Fermentation medium be sucrose 1%, peptone 1%, disodium hydrogen phosphate 0.2%, sodium dihydrogen phosphate 0.2%,
pH7.0。
The application of rejected layer meat improved formulations is advisable according to feed total weight with 0.1%~0.3% additive amount,
The improved formulations are added in rejected layer feed.
Rhodotorula mucilaginose, S. cervisiae, bacillus subtilis, which have, generates organic acid, digestive ferment, lysozyme, special intestines
The characteristics such as wall adhesive capacity play many physiological functions in body, such as improve intestinal health, improve immunity of organisms, inhibit
The harmful bacterias such as salmonella, Escherichia coli reduce blood cholesterol levels, blood lipid etc..Rhodotorula mucilaginose, S. cervisiae, withered grass bud
Excellent substitute of the spore bacillus as antibiotic, also has improvement result to livestock and poultry meat.
Plants essential oil has natural bioactivity substance abundant, and adjustable animal immune, anti-oxidant and metabolism etc. are raw
Function is managed, is a kind of safe and efficient, noresidue green feed additive.Most of stronger plant essences of antibacterial ability
All contain quite a high proportion of phenols or aldehyde compound, such as common carvacrol, Thymol, eugenol and cinnamic acid in oil
Deng.Plants essential oil has broad spectrum activity to the inhibiting effect of bacterium, while having extensive physiological function, such as sterilizes, is antiviral, anti-
Oxidation, anti-inflammatory etc..
Vitamin E in the present invention primarily serves free radical resisting, and antioxidation reduces the effect of chicken drip loss.
The utility model has the advantages that the present invention is suitable for possessing the scale laying hen of 400 ages in days and the above laying hen, supported using no cageization
Grow mode.Meat modifier of the invention is fed, puts down and supports, and laying hen is allowed to have certain sports center, in a controllable environment
In walk around and inhabit, it is ensured that health and safety.Using computer Precision management environment, the ecotopia for meeting laying hen is wanted
Ask (mainly temperature, humidity and ammonia concentration).Using the feeding facility of automation, the drinking-water system of nipple-shape is installed to ensure
Reliable free of contamination water source；Automatic feeding system needs to adjust scale of feeding according to laying hen；Using high reliablity, operation letter
Just, egg conveyer system softly；Automatic chicken manure collection system.It standardizes sound epidemic prevention system, scientific immune programme and exempts from
Epidemic disease mode and perfect innocuity disposal system.Authoritative testing agency's detection meat is cultivated with verifying scale laying hen without cageization
The application effect of meat improving environment mode, significantly improves the content of inosinicacid and intramuscular fat in laying hen muscle, makes chicken
Meat cunning is tender tasty and refreshing.
Specific embodiment
Technical solution of the present invention is illustrated below with reference to embodiment:
One, prepared by rejected layer meat modifier
The preparation of rejected layer meat modifier carries out (unit in table 1 is parts by weight) by component formula used by table 1:
Table 1: rejected layer meat modifier formula composition
Preparation method: being to be uniformly mixed above-mentioned each component, makes its uniformity coefficient of variation CV less than 5% to get to institute
State meat modifier.It is used for zoopery simultaneously, verifies its application effect.
Two, zoopery:
1, experimental animal and grouping
(1) animal: 400 ages in days eliminate the brown laying hen of Luo Man 3600, and by the poultry of Nanjing spring mind, cooperative society is provided.
(2) 3 groups, every group 1200 animal packet: are randomly divided into.Basal diet trophic component is according to NRC about the mother that lays eggs
Nutrition needed for chicken is matched, and see Table 2 for details.Preliminary trial period 1 week, after confirming each group laying rate, production performance no significant difference, formally
Test 35d.
(3) layer diets formula composition and dosage:
Table 2: feeding Diet Formula composition
| Kind | Control group (basal diet) | Test group 1 | Test group 2 |
| Corn (%) | 63.0 | 63.0 | 63.0 |
| Soybean Meal (%) | 35.0 | 35.0 | 35.0 |
| Mountain flour (%) | 7 | 6.9 | 6.5 |
| Premix (%) | 5 | 5 | 5 |
| Meat improved formulations (%) | — | 0.1 | 0.5 |
| Total (%) | 100 | 100 | 100 |
2, the acquisition and processing of sample
5 chickens are randomly selected from each test group every 7d to be butchered, and measure nutriment in chicken leg and Fresh Grade Breast respectively
Content and medicament residue.
3, result
Nutrition content is as shown in table 3 in chicken leg and Fresh Grade Breast.
Table 3: nutrition content in chicken leg and Fresh Grade Breast
Interpretation of result: after the modifying agent for adding the application, inosinicacid and intramuscular fat in laying hen muscle are significantly improved
Content keeps chicken cunning tender tasty and refreshing.But protein, calcium, zinc difference with insignificance, while being studied through Jiangsu Province's food and medicine supervision and inspection
Institute's detection, Enrofloxacin, Ciprofloxacin, Florfenicol, erythromycin, fortimicin, furanone metabolites, furantoin metabolism
Object, Furacilin metabolite, Furaxone metabolite, sulphadiazine, sulfadimidine, daimeton, sulphur ammonium two
First isoxazole, fanasil etc. are not detected.
Claims (4)
1. a kind of rejected layer meat improved formulations, it is characterised in that include Rhodotorula mucilaginose, S. cervisiae, bacillus subtilis
The parts by weight of bacterium, plants essential oil and vitamin E, each component are as follows:
Rhodotorula mucilaginose: 20-60 parts
S. cervisiae: 3-30 parts
Bacillus subtilis: 5-40 parts
Plants essential oil: 5-40 parts
Vitamin E: 3-20 parts.
2. rejected layer meat modifier according to claim 1, it is characterised in that: the parts by weight of each component are as follows:
Rhodotorula mucilaginose: 30-50 parts；
S. cervisiae: 5-20 parts；
Bacillus subtilis: 10-30 parts；
Plants essential oil: 10-30 parts；
Vitamin E: 5-10 parts.
3. rejected layer meat modifier according to claim 1, it is characterised in that the Rhodotorula mucilaginose, wine brewing ferment
Female bacterium, bacillus subtilis are dry powder formulations, and the pure bacterium powder viable count of every kind of bacterial strain should be not less than l ~ 5 × 109cfu/g。
4. the application of rejected layer meat improved formulations described in claim 1, be according to feed total weight with 0.1% ~
The improved formulations are added in animal and fowl fodder by 0.3% additive amount.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910276731.1A CN109965136A (en) | 2019-04-08 | 2019-04-08 | A kind of rejected layer meat improved formulations, preparation method and its application |
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| CN201910276731.1A CN109965136A (en) | 2019-04-08 | 2019-04-08 | A kind of rejected layer meat improved formulations, preparation method and its application |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113142416A (en) * | 2021-04-01 | 2021-07-23 | 薛松晓 | Biological preparation for improving meat quality of rejected laying hens and preparation method and application thereof |
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| CN103478443A (en) * | 2013-08-20 | 2014-01-01 | 沈阳金科丰牧业科技有限公司 | Micro-ecological preparation and application thereof in improvement of production performance, immune performance as well as chicken quality of broiler |
| CN104206794A (en) * | 2014-06-26 | 2014-12-17 | 湖北绿科乐华生物科技有限公司 | Biological feed for improving flesh quality of obsolete laying hen and producing method thereof |
| CN106010990A (en) * | 2016-05-13 | 2016-10-12 | 西南大学 | Rhodotorula mucilaginosa and fermentation culture substance and application thereof |
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2019
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113142416A (en) * | 2021-04-01 | 2021-07-23 | 薛松晓 | Biological preparation for improving meat quality of rejected laying hens and preparation method and application thereof |
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