CN109959649B - Cigarette tar extract with chemiluminescence property and chemiluminescence system thereof - Google Patents

Cigarette tar extract with chemiluminescence property and chemiluminescence system thereof Download PDF

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CN109959649B
CN109959649B CN201711430018.5A CN201711430018A CN109959649B CN 109959649 B CN109959649 B CN 109959649B CN 201711430018 A CN201711430018 A CN 201711430018A CN 109959649 B CN109959649 B CN 109959649B
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chemiluminescence
cigarette tar
tar extract
extract
cigarette
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CN109959649A (en
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王大彬
孔凡玉
邱军
于卫松
庞雪莉
曹建敏
方松
鲁世军
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Tobacco Research Institute of Hubei Province
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01D11/00—Solvent extraction
    • B01D11/04—Solvent extraction of solutions which are liquid
    • B01D11/0419—Solvent extraction of solutions which are liquid in combination with an electric or magnetic field or with vibrations
    • B01D11/0423—Applying ultrasound
    • G—PHYSICS
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    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence

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Abstract

本发明公开了一种具有化学发光性质的卷烟焦油提取物及其化学发光体系。该卷烟焦油提取物的制备方法，包括如下步骤：将卷烟焦油加入试剂进行提取，得到卷烟焦油提取物。本发明卷烟焦油提取物是在自然光条件下的敞开体系中通过超声波提取制得的，因此包含的发光物质性质稳定，对光照、氧气及温度等因素不敏感；该提取物对介质pH值的发光响应范围宽，在酸性、中性或碱性条件下都可以发生化学发光反应。本发明卷烟焦油提取的原料来源广泛，制作方法简单。本发明卷烟焦油提取物在化学发光分析检测技术领域有广泛的应用前景。

Figure 201711430018

The invention discloses a cigarette tar extract with chemiluminescence properties and a chemiluminescence system thereof. The preparation method of the cigarette tar extract includes the following steps: adding the cigarette tar into a reagent for extraction to obtain the cigarette tar extract. The cigarette tar extract of the present invention is prepared by ultrasonic extraction in an open system under natural light conditions, so the luminescent substances contained are stable in nature and are insensitive to factors such as illumination, oxygen and temperature; the extract has luminescence on the pH value of the medium. The response range is wide, and the chemiluminescence reaction can occur under acidic, neutral or basic conditions. The raw materials extracted from the cigarette tar of the invention come from a wide range of sources, and the preparation method is simple. The cigarette tar extract of the invention has wide application prospects in the technical field of chemiluminescence analysis and detection.

Figure 201711430018

Description

Cigarette tar extract with chemiluminescence property and chemiluminescence system thereof
Technical Field
The invention relates to a chemiluminescent substance, in particular to a cigarette tar extract with chemiluminescent property and a chemiluminescent system thereof, belonging to the technical field of chemiluminescent analysis and detection.
Background
Chemiluminescence refers to a light emission phenomenon that occurs when a reaction intermediate or a reaction product in an excited state of electrons is transferred from the excited state to a ground state by absorbing chemical energy released by the reaction in a chemical reaction. Chemiluminescence is an excellent analysis and detection technology, has the advantages of mild reaction conditions, simple instrument and device, convenient operation, high sensitivity and the like, and is widely applied in the fields of environmental monitoring, drug analysis, food inspection, biological analysis, clinical medicine and the like. However, with the increasing public health and safety awareness, the analysis requirements of various fields are increasing, and the existing chemiluminescence analysis technology cannot meet the current analysis requirements. Therefore, development of a new analytical technique is an urgent necessity to expand the analytical application of the chemiluminescence technique in various fields. The chemiluminescence probe is a core element for constructing chemiluminescence analysis technology, and the existing luminescence probe often has the following defects: (1) the limited number makes it difficult to construct new luminescent systems around existing luminescent probes for analytical detection; (2) most of the existing luminescent probes are unstable in property and are easily influenced by external conditions such as oxygen, illumination, temperature and the like when in use; (3) the probe luminescence reaction has strong dependence on the pH value of the system. For example, some luminescent probes have chemiluminescence reaction only in alkaline environment, but difficult to perform under neutral and acidic conditions, thereby limiting their application in neutral and acidic systems. Therefore, the discovery of novel chemiluminescent substances is an important way for the development of chemiluminescence analysis and detection applications.
Disclosure of Invention
The invention aims to provide a cigarette tar extract with chemiluminescence property and a chemiluminescence system thereof, wherein the cigarette tar extract with chemiluminescence property has stable property and is insensitive to factors such as illumination, oxygen, temperature and the like; the luminous response range to the pH value of the medium is wide, and the chemiluminescence reaction can be carried out under the acidic, neutral or alkaline conditions.
The invention provides a preparation method of a cigarette tar extract, which comprises the following steps: adding cigarette tar into the reagent for extraction to obtain the cigarette tar extract.
In the above preparation method, the ratio of the cigarette tar to the reagent may be 400 mg: (20-150) mL, specifically 400 mg: 40 mL.
In the preparation method, the cigarette tar can be prepared by the following steps: smoking the cigarette, filtering the generated smoke, and collecting the smoke particle phase substances to obtain the cigarette tar.
The cigarette can be a baked cigarette, in particular to a baked cigarette without a filter tip which is purchased from commercial sources, or a baked cigarette which is prepared by adopting the existing preparation method; in a specific embodiment of the present invention, the flue-cured type cigarette is a cigarette prepared by the following steps: (1) baking and modulating the tobacco leaves; (2) removing fine soil, sand grains and dust on the cured and modulated tobacco leaves, dampening for 6-12 hours at the temperature higher than 22 ℃, removing main ribs of the tobacco leaves, shredding, and rolling the shredded tobacco leaves to obtain cigarettes. The baking modulation can adopt a three-stage (three stages of yellowing, fixing color and drying tendon) baking process, and comprises the following specific steps: in the yellowing stage, the tobacco leaves collected in the field are woven and bound and loaded into a kang, after ignition, the temperature of a curing barn is increased to 36-38 ℃ (such as 38 ℃) at the rate of 1 ℃ per hour, the temperature of a wet bulb is kept to be 1-2.5 ℃ (such as 1.5 ℃) lower than that of a dry bulb until more than 80% of the tobacco leaves in a bottom shed change to be about eighty percent yellow, then the temperature of the curing barn is increased to 40-42 ℃ (such as 42 ℃), the temperature of the wet bulb is kept to be 36-37 ℃ (such as 37 ℃), and appropriate moisture removal is carried out, so that the tobacco leaves are ensured to reach the yellowing and softening state; in the color fixing stage, the moisture removal amount is increased, the temperature of the baking room is increased to 54-55 ℃ (such as 55 ℃) at the rate of increasing 1 ℃ for 2-3 hours (such as 2 hours), the temperature of a wet bulb is slowly increased and kept at 37-40 ℃ (such as 38 ℃), the leaves are dried, and the yellow color is fixed; in the gluten drying stage, the temperature of the curing barn is increased to 67-69 ℃ (such as 69 ℃) at the rate of 1 ℃ per hour, the temperature of the wet bulb is kept at about 41-42 ℃ (such as 42 ℃), and the fire is stopped after the leaves are fixed and dried.
The suction may be by a smoking machine. The filtration can adopt a Cambridge filter sheet. The ratio of the cigarette to the cigarette tar can be specifically 20: 400 mg.
In the above preparation method, the reagent may be at least one of methanol, water, ethyl acetate, ethanol, acetone, dichloromethane and chloroform.
In the above preparation method, the extraction may be ultrasonic extraction.
The conditions for the ultrasonic extraction may be as follows: the temperature is less than 70 ℃ (such as 15-30 ℃, 25 ℃), the time is 10 min-30 min (such as 20min), and the power is 200-500W (such as 400W).
In the above preparation method, the method further comprises, after the extracting, a step of filtering the obtained extract and collecting the filtrate. The filtration adopts a filter membrane. The filter membrane may be an organic phase filter membrane or an aqueous phase filter membrane. The pore size of the filter membrane is less than 420 μm, and specifically can be 0.22 μm or 0.45 μm which is commonly used in laboratories.
The invention further provides the cigarette tar extract prepared by the preparation method.
The application of the cigarette tar extract in serving as a chemiluminescent substance or preparing a chemiluminescent system is also within the protection scope of the invention.
The invention also provides a chemiluminescence system which comprises the cigarette tar extract.
In the chemiluminescence system, the mass concentration of the cigarette tar extract can be 1.0 mg/mL-8.0 mg/mL, and specifically can be 4.0 mg/mL.
The chemical luminous system is any one of the following A) to D):
A) comprises the cigarette tar extract and an oxidant or an oxidation system;
B) consists of the cigarette tar extract and an oxidant or an oxidation system;
C) comprises the cigarette tar extract and enzyme-H2O2A system;
D) the tar extract of the cigarette and enzyme-H2O2The system is formed.
The oxidant can be H2O2、KIO4And NaClO; the oxidation system may be a Fenton system (Fe)2+-H2O2System) is provided. The enzyme-H2O2The system can be HRP-H2O2System (Horseradish peroxidase-H)2O2System) is provided.
In particular, the chemiluminescent system a) may be any one of the following a1) -A3):
A1) comprises the cigarette tar extract and H2O2；
A2) Comprises the cigarette tar extract and KIO4Or NaClO;
A3) comprises the cigarette tar extract and a Fenton system;
the chemiluminescent system B) may be any one of the following B1) -B3):
B1) the tar extract of the cigarette and H2O2Forming;
B2) the cigarette tar extract and KIO4Or NaClO;
B3) consists of the cigarette tar extract and a Fenton system.
The chemiluminescence system comprises the cigarette tar extract and H2O2The chemical luminous system of (A) or (B) the cigarette tar extract and H2O2In the formed chemiluminescence system, the mass concentration of the cigarette tar extract can be 1.0 mg/mL-8.0 mg/mL, and specifically can be 4.0 mg/mL; h2O2The molar concentration of (b) may be 0.1 to 1.0mol/L, specifically 1.0 mmol/L.
The chemiluminescence system comprises the cigarette tar extract and KIO4Or a chemiluminescent system of NaClO or the combination of said cigarette tar extract and KIO4Or in a chemiluminescence system formed by NaClO, the mass concentration of the cigarette tar extract can be 1.0 mg/mL-8.0 mg/mL, and specifically can be 4.0 mg/mL; KIO4Or the molar concentration of NaClO can be 0.1 mmol/L-1.0 mol/L, and specifically can be 1.0 mmol/L.
In the chemiluminescence system, the mass concentration of the cigarette tar extract can be 1.0 mg/mL-8.0 mg/mL, and particularly can be 4.0mg/mL, and the chemiluminescence system comprises the cigarette tar extract and a Fenton system or comprises the cigarette tar extract and the Fenton system; fe in Fenton system2+May be in a molar concentration of10 mu mol/L-0.01 mol/L, and can be 0.1mmol/L specifically; h2O2The molar concentration of (b) may be 0.1 to 0.1mol/L, specifically 1.0 mmol/L.
The chemiluminescence system comprises the cigarette tar extract and enzyme-H2O2The chemiluminescence system of the system or the cigarette tar extract and enzyme-H2O2In a chemiluminescence system formed by the system, the mass concentration of the cigarette tar extract can be 1.0 mg/mL-8.0 mg/mL, and specifically can be 4.0 mg/mL; enzyme-H2O2The mass concentration of the enzyme in the system can be 0.01 mg/mL-0.1 mg/mL, and specifically can be 0.1 mg/mL; h2O2The molar concentration of (b) may be 1.0 to 0.1mol/L, specifically 1.0 mmol/L.
The detection application of the cigarette tar extract and the chemical luminous system in acidic, neutral or alkaline media is also within the protection scope of the invention.
The invention has the following beneficial effects:
the cigarette tar extract is prepared by ultrasonic extraction in an open system under natural light conditions, so that the contained luminescent substance has stable property and is insensitive to factors such as illumination, oxygen, temperature and the like; the extract has wide luminescence response range to medium pH value, and can generate chemiluminescence reaction under acidic, neutral or alkaline conditions. The raw material source for extracting the cigarette tar is wide, and the preparation method is simple. The cigarette tar extract has wide application prospect in the technical field of chemiluminescence analysis and detection.
Drawings
FIG. 1 shows the tobacco tar extracts and different concentrations H of the cigarette of example 12SO4And NaOH, wherein FIG. 1(a) is a graph of different concentrations of H2SO4Each reference numeral indicates as follows: (1)1.0mol/L, (2)0.1mol/L and (3) 1X 10- 2mol/L，(4)1×10-3mol/L，(5)1×10-4mol/L，(6)1×10-5mol/L; FIG. 1(b) shows NaOH in different concentrations, and the reference numerals are as follows: (1) 1X 10-5mol/L，(2)1×10-4mol/L，(3)1×10-3mol/L，(4)1×10-2mol/L，(5)0.1mol/L，(6)1.0mol/L。
FIG. 2 shows the combination of cigarette tar extract and H under acidic and alkaline conditions, respectively, in example 12O2The chemiluminescence kinetic curve of (1), wherein FIG. 2(a) is H at different concentrations2SO4Each reference numeral indicates as follows: (1) 1X 10-5mol/L，(2)1×10- 4mol/L，(3)1×10-3mol/L，(4)1×10-2mol/L (5)0.1mol/L, (6)1.0 mol/L; FIG. 2(b) shows NaOH in different concentrations, and the reference numerals are as follows: (1) 1X 10-3mol/L，(2)1×10-2mol/L，(3)0.1mol/L，(4)1.0mol/L。
FIG. 3 is the chemiluminescence kinetics curves of the cigarette tar extract of example 1 and two common reagents in acidic, neutral and alkaline media, wherein the reagent of FIG. 3(a) is KIO4Each reference numeral indicates as follows: (1)0.1mol/L H2SO4，(2)H2O，(3)1×10-5mol/L NaOH; the reagent in FIG. 3(b) is NaClO, and the reference numerals denote the following: (1)0.1mol/L H2SO4，(2)H2O，(3)1×10-5mol/L NaOH。
FIG. 4 shows the pH of the tobacco tar extract of example 1 with Fe at different pH values2+-H2O2Chemiluminescence kinetics curves for the reagent reactions, each index being as follows: (1)1.0mol/L H2SO4，(2)0.1mol/L H2SO4，(3)0.01mol/L H2SO4，(4)1×10-3mol/L H2SO4，(5)1×10-4mol/L H2SO4，(6)1×10-5mol/L H2SO4，(7)1×10- 6mol/L H2SO4(8) pure water, (9) 1X 10-6mol/L NaOH，(10)1×10-5mol/L NaOH，(11)1×10-4mol/L NaOH，(12)1×10-3mol/L NaOH，(13)0.01mol/L NaOH，(14)0.1mol/L NaOH，(15)1.0mol/L NaOH。
FIG. 5 shows the tobacco tar extract and HRP-H in example 12O2In acidic, neutral and basic mediaChemiluminescence kinetics curves, each index being as follows: (1) 1X 10-4mol/L H2SO4，(2)H2O，(3)1×10-5mol/L NaOH。
FIG. 6 is a graph of the chemiluminescence kinetics of the cigarette tar extract (concentration of 1.2mg/mL) of example 2 with different concentrations of NaOH, each of which is indicated below: (1)0mol/L (2) 1X 10-6mol/L，(3)1×10-5mol/L，(4)1×10- 4mol/L，(5)1×10-3mol/L，(6)0.01mol/L，(7)0.1mol/L，(8)1.0mol/L。
Detailed Description
The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.
Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
The cigarettes used in the following examples were self-rolled in the laboratory, and the specific steps were as follows:
(1) the method adopts a three-stage (three stages of yellowing, fixing color and drying tendon) baking process to bake and modulate the tobacco leaves, and comprises the following specific steps: in the yellowing stage, the tobacco leaves collected in the field are woven and bound and loaded into a kang, the temperature of a curing barn is increased to 38 ℃ at the rate of 1 ℃ per hour after ignition, the temperature of a wet bulb is kept lower than that of a dry bulb by 1.5 ℃ until more than 80% of the tobacco leaves in a bottom shed are changed to be eighty percent yellow or so, then the temperature of the curing barn is increased to 42 ℃, the temperature of the wet bulb is kept at 37 ℃, and proper moisture removal is carried out to ensure that the tobacco leaves reach the yellowing and softening state; in the fixing stage, the moisture removal amount is increased, the temperature of the baking room is increased to 55 ℃ at the rate of 2 hours of 1 ℃, the temperature of a wet bulb is slowly increased and kept at 38 ℃, the leaves are dried, and the yellow color is fixed; in the tendon drying stage, the temperature of the curing barn is increased to 69 ℃ at the rate of 1 ℃ per hour, the temperature of a wet bulb is kept at about 42 ℃, and the fire is stopped after the leaves are fixed in color and dried;
(2) brushing fine soil, sand grains and dust on the tobacco leaves baked and modulated in the step (1) by using a soft hair brush, dampening the tobacco leaves for 10 hours at room temperature, removing main ribs of the tobacco leaves, shredding the tobacco leaves, and rolling the tobacco leaves after shredding to ensure that each cigarette has the length of 70mm, the circumference of 27.5mm and the weight of 1.1 +/-0.1 g; the tar content of each cigarette is 20 mg.
The smoking machine used in the examples described below was manufactured by Borgwaldt, Germany, under the model RM20/cs, and the Cambridge filter was manufactured by Borgwaldt, Germany, under the model 80202841; the organic phase filter membrane is manufactured by Shanghai' an spectral laboratory science and technology company with the model of SCAA-103. The water phase filter membrane is manufactured by Shanghai' an spectral experiment science and technology company with the model of SCAA-101.
The luminescence kinetics curves in the following examples were obtained by means of static injection in a faint luminescence apparatus (BPCL-GP15-TGC) at a negative high pressure of-1000V.
The preparation of the cigarette tar extract having chemiluminescent property in the following examples was carried out in an open system under natural light conditions at room temperature (25 ℃ C.), unless otherwise specified.
Example 1 preparation of cigarette Tar extract with chemiluminescent Properties (methanol as reagent)
First, preparation method
Preparing cigarette tar extract according to the following steps:
smoking 20 cigarettes at a time by using a smoking machine, and intercepting smoke particulate matters in the smoke obtained by smoking through a Cambridge filter disc to obtain the Cambridge filter disc loaded with cigarette tar; cutting Cambridge filter into strips, adding into 40mL methanol, performing ultrasonic extraction for 20min (ultrasonic power: 400W), filtering with 0.45 μm organic phase filter membrane, and collecting filtrate to obtain cigarette tar extract.
II, chemiluminescence property
1. Chemiluminescence phenomenon of cigarette tar extract on strong acid and strong base
Respectively measuring H of the cigarette tar extract at different concentrations2SO4And a chemiluminescence kinetic curve in NaOH (the mass concentration of the cigarette tar extract is 4.0mg/mL), and the experimental result is shown in figure 1. As can be seen from FIG. 1(a), the concentration of sulfuric acid is 1X 10-5In the range of mol/L-1.0 mol/L, the tar extract of the invention has chemiluminescence response.As can be seen from FIG. 1(b), in the strongly alkaline medium, the tar extract has chemiluminescence response at a sodium hydroxide concentration of 0.1mol/L, and the luminescence intensity generated at a sodium hydroxide concentration of 1.0mol/L is much greater than that at 0.1 mol/L. Thus, the tar extract of the present invention has a chemiluminescent response in both strong acid and strong base media.
2. Under acidic and basic conditions with H2O2Chemical luminescence phenomenon of
Respectively measuring the tar extract and H of the cigarette2O2The formed chemiluminescence system (the mass concentration of cigarette tar extract is 4.0mg/mL, H2O2At a molar concentration of 1.0mmol/L) of H at different concentrations2SO4And chemiluminescence kinetics curves in NaOH, the experimental results are shown in fig. 2. As can be seen from FIG. 2(a), when the sulfuric acid concentration is 1X 10- 2When the mol/L is within the range of 1.0mol/L, the tar extract and the hydrogen peroxide have obvious chemiluminescent signals; as can be seen from FIG. 2(b), in the alkaline medium, when the sodium hydroxide concentration is 1X 10 respectively-5At mol/L and 1.0mol/L, the tar extract of the invention has obvious luminous signals with hydrogen peroxide. The experiment shows that the tar extract of the invention can react with H in both acidic and alkaline environments2O2A chemiluminescent reaction occurs. Thus, the tar extract of the present invention may be based on tar extract-H2O2Chemiluminescent systems are established for the detection of substances in acidic and basic media.
3. Cigarette tar extract and KIO4Or the chemiluminescence phenomenon of NaClO in acidic, neutral and alkaline media
Respectively measuring the tar extract and KIO of the cigarette4Or a chemiluminescence system composed of NaClO (the mass concentration of cigarette tar extract is 4.0 mg/mL; KIO)4Has a molar concentration of 1.0mmol/L and a molar concentration of NaClO of 1.0mmol/L) in H2SO4、H2Chemiluminescence kinetics curves in O and NaOH, and the experimental results are shown in fig. 3. As can be seen in FIG. 3, the two reagents are each at H2SO4(0.1mol/L)、H2O and NaOH (1X 10)-5mol/L) in the medium with tar extract can generate chemiluminescence phenomenon. Therefore, the luminophore system based on the tar extract and the two reaction reagents has potential application value in the aspect of analysis and detection.
4. Chemiluminescence phenomenon of tar extract and Fenton system under different pH values
Respectively measuring the chemiluminescence system formed by the prepared cigarette tar extract and Fenton system (the mass concentration of the cigarette tar extract is 4.0 mg/mL; Fe2+-H2O2Fe in the system2+Has a molar concentration of 0.1mmol/L, H2O2At a molar concentration of 1.0mmol/L), of H at different concentrations2SO4And chemiluminescence kinetics curves in NaOH, the experimental results are shown in fig. 4. As can be seen from FIG. 4, the tar extract and Fe of the present invention are present in the medium pH range of 0-142+-H2O2The system has chemiluminescence signals, which show that the tar extract can generate chemiluminescence phenomenon with a Fenton system under acidic, neutral and alkaline conditions, and hydroxyl radicals are main active species in the Fenton system, so that the hydroxyl radicals can be reaction species causing the tar extract to emit light.
5. Enzyme (HRP) -H2O2Chemiluminescence phenomenon of system and tar extract under acidic, neutral and alkaline conditions
Respectively measuring the prepared cigarette tar extract and enzyme (HRP) -H2O2The chemiluminescence system (the mass concentration of cigarette tar extract is 4.0 mg/mL; enzyme (HRP) -H)2O2The mass concentration of HRP in the system is 0.1mg/mL, H2O2Molar concentration of 1.0mmol/L) in H2SO4、H2Chemiluminescence kinetics curves in O and NaOH, and the experimental results are shown in fig. 5. As can be seen from FIG. 5, the tar extract of the present invention was mixed with HRP-H2O2System H2SO4(1×10-4mol/L)、H2O and NaOH (1X 10)-5mol/L) medium, and therefore the invention can be based on tar extract-HRP-H2O2Luminescent systems were established for analytical detection in acidic, neutral and basic media.
Example 2 preparation of cigarette Tar extract (reagent Water) with chemiluminescent Properties
First, preparation method
Preparing cigarette tar extract according to the following steps:
smoking 20 cigarettes at a time by using a smoking machine, and intercepting smoke particulate matters in the smoke obtained by smoking through a Cambridge filter disc to obtain the Cambridge filter disc loaded with cigarette tar; after the completion, the Cambridge filter is cut into strips and added into 40mL of water for ultrasonic extraction for 20min (ultrasonic power: 400W), and the strips are filtered through a water-phase filter membrane of 0.45 mu m, and finally the filtrate is collected to obtain the cigarette tar extract.
II, chemiluminescence property
1. Chemiluminescence phenomenon of cigarette tar extract to strong alkali
The chemiluminescence kinetic curves of the prepared cigarette tar extract in NaOH with different concentrations (the mass concentration of the cigarette tar extract is 1.2mg/mL) are measured, and the experimental results are shown in FIG. 6. As can be seen from FIG. 6, in the strongly alkaline medium, the tar extracts all have chemiluminescence response when the concentration of sodium hydroxide is 0.01 mol/L-1.0 mol/L.

Claims (5)

1.一种化学发光体系，其特征在于：所述化学发光体系为下述A)或B)：1. a chemiluminescence system, is characterized in that: described chemiluminescence system is following A) or B): A)由卷烟焦油提取物与氧化剂或氧化体系构成；A) consists of cigarette tar extract and oxidant or oxidation system; B)由卷烟焦油提取物与酶-H2O2体系构成；B) consists of cigarette tar extract and enzyme-H 2 O 2 system; 所述卷烟焦油提取物的制备方法，包括如下步骤：将卷烟焦油加入试剂进行提取，得到卷烟焦油提取物；The preparation method of the cigarette tar extract comprises the following steps: adding the cigarette tar into a reagent for extraction to obtain the cigarette tar extract; 所述试剂为甲醇和/或水；The reagent is methanol and/or water; 所述氧化剂为H2O2、KIO4和NaClO中的任一种；所述氧化体系为Fenton体系；所述酶-H2O2体系为HRP-H2O2体系。The oxidant is any one of H 2 O 2 , KIO 4 and NaClO; the oxidation system is a Fenton system; the enzyme-H 2 O 2 system is an HRP-H 2 O 2 system. 2.根据权利要求1所述的化学发光体系，其特征在于：所述卷烟焦油与所述试剂的比例为400mg：(20～150)mL。2 . The chemiluminescence system according to claim 1 , wherein the ratio of the cigarette tar to the reagent is 400 mg: (20-150) mL. 3 . 3.根据权利要求1或2所述的化学发光体系，其特征在于：所述提取为超声波提取；和/或，3. The chemiluminescence system according to claim 1 or 2, wherein the extraction is ultrasonic extraction; and/or, 所述超声波提取的条件如下：温度为小于70℃，时间为10min～30min，功率为200～500W。The conditions of the ultrasonic extraction are as follows: the temperature is less than 70° C., the time is 10min-30min, and the power is 200-500W. 4.根据权利要求1或2所述的化学发光体系，其特征在于：所述方法在所述提取之后还包括对得到的提取液进行过滤并收集滤液的步骤；和/或，4. The chemiluminescence system according to claim 1 or 2, wherein the method further comprises the step of filtering the obtained extract and collecting the filtrate after the extraction; and/or, 所述过滤采用滤膜；和/或，The filtration uses a filter membrane; and/or, 所述滤膜的孔径小于420μm。The pore size of the filter membrane is less than 420 μm. 5.权利要求1-4中任一项所述的化学发光体系在酸性、中性或碱性介质中的检测应用。5. The detection application of the chemiluminescence system according to any one of claims 1 to 4 in acidic, neutral or basic media.
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