CN109959649A - A kind of coke tar in cigarette extract and its chemical luminous system with chemiluminescence property - Google Patents
A kind of coke tar in cigarette extract and its chemical luminous system with chemiluminescence property Download PDFInfo
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- CN109959649A CN109959649A CN201711430018.5A CN201711430018A CN109959649A CN 109959649 A CN109959649 A CN 109959649A CN 201711430018 A CN201711430018 A CN 201711430018A CN 109959649 A CN109959649 A CN 109959649A
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- B01D11/04—Solvent extraction of solutions which are liquid
- B01D11/0419—Solvent extraction of solutions which are liquid in combination with an electric or magnetic field or with vibrations
- B01D11/0423—Applying ultrasound
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- G—PHYSICS
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- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
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Abstract
The invention discloses a kind of coke tar in cigarette extracts and its chemical luminous system with chemiluminescence property.The preparation method of the coke tar in cigarette extract includes the following steps: to extract coke tar in cigarette addition reagent, obtains coke tar in cigarette extract.Coke tar in cigarette extract of the present invention is in unlimited system under the conditions of natural light as made from ultrasonic wave extraction, therefore the luminescent substance property for including is stablized, insensitive to factors such as illumination, oxygen and temperature；The extract is wide to the luminescence response range of PH values, and chemiluminescence reaction can occur under acid, neutral or alkaline condition.The raw material sources that coke tar in cigarette of the present invention extracts are extensive, and production method is simple.Coke tar in cigarette extract of the present invention has wide practical use in chemiluminescence analysis detection technique field.
Description
Technical field
The present invention relates to chemiluminescent substance more particularly to a kind of coke tar in cigarette extract with chemiluminescence property and
Its chemical luminous system belongs to chemiluminescence analysis detection technique field.
Background technique
Chemiluminescence refers to the chemical energy that reaction release is absorbed in chemical reaction and the reaction in excited electronic state
A kind of light radiation phenomenon that intermediate or reaction product generate when moving to ground state by excitation state.Chemiluminescence is a kind of excellent
Analysis and detection technology has many advantages, such as that reaction condition is mild, apparatus is simple and convenient to operate, high sensitivity, supervises in environment
The fields such as survey, Pharmaceutical Analysis, Food Inspection, bioanalysis and clinical medicine are widely used.However, as public health is anticipated safely
The analysis demand of the continuous improvement of knowledge, every field increasingly increases, and existing chemiluminescence analytical technique is unable to satisfy current point
Analysis demand.Therefore developing new analytical technology becomes expansion chemiluminescence in the task of top priority of every field analysis application.
Chemiluminescence probe is the key element for constructing chemiluminescence analytical technique, and existing luminescence probe is frequently present of following deficiency:
(1) limited amount leads to be difficult to construct novel luminescence system around existing luminescence probe for analysis detection；(2) existing to shine
The most property of probe is unstable, is influenced when use vulnerable to external conditions such as oxygen, illumination and temperature；(3) probe luminescence-producing reaction pair
The dependence of system pH is strong.Such as the chemiluminescence reaction of some luminescence probes can only occur in alkaline environment, and in
Property and acid condition under be difficult to carry out, to limit its application in neutral and acid system.Therefore new chemical is excavated
Luminescent substance is the important channel of current developing chemiluminescence analysis detection application.
Summary of the invention
The object of the present invention is to provide a kind of coke tar in cigarette extracts and its chemical luminophor with chemiluminescence property
System, there is the coke tar in cigarette extract property of chemiluminescence property to stablize for this, insensitive to factors such as illumination, oxygen and temperature；
It is wide to the luminescence response range of PH values, chemiluminescence reaction can occur under acid, neutral or alkaline condition.
A kind of preparation method of coke tar in cigarette extract provided by the invention, includes the following steps: for coke tar in cigarette to be added
It is extracted in reagent, obtains coke tar in cigarette extract.
In above-mentioned preparation method, the ratio of the coke tar in cigarette and the reagent can be 400mg:(20~150) mL, tool
Body can be 400mg:40mL.
In above-mentioned preparation method, the coke tar in cigarette can be made by the steps to obtain: smoking cigarette, to generation
Flue gas is filtered, and is collected Smoke Particulate, is obtained coke tar in cigarette.
The cigarette can be baking-type cigarette, the fire-cured tobacco type volume that concretely commercial sources were commercially available remove filter tip
Cigarette can also be the Virginian-type cigarette being prepared using existing preparation method；In a specific embodiment of the present invention, described roasting
Tobacco type of cigarette is the cigarette being prepared using following steps: (1) tobacco leaf being carried out baking modulation；(2) baking is removed to adjust
The fine earth on tobacco leaf, sand grains and dust after system get damp again 6~12 hours under the conditions of being higher than 22 DEG C, cut after removing tobacco leaf main reinforcement
Silk, rolls it after chopping, obtains cigarette." three-stage (flavescence, fixation and three, dry muscle can be used in the baking modulation
Stage) " baking process, the specific steps are as follows: in yellowing stage, by the tobacco leaf binding of field acquisition, dress a heatable brick bed, with every small after igniting
When 1 DEG C of rate barn temperature is risen to 36~38 DEG C (such as 38 DEG C), keep wet-bulb temperature it is lower than dry-bulb temperature 1~2.5 DEG C (such as
1.5 DEG C), it is controlled until 80% or more bottom canopy tobacco leaf changes to eighty per cant Huang, barn temperature is then increased to 40~42 DEG C (such as 42
DEG C), it keeps 36~37 DEG C of wet-bulb temperature (such as 37 DEG C), and appropriate hydrofuge, it is ensured that tobacco leaf reaches flavescence softening state；In fixation rank
Section increases hydrofuge amount, and the rate for rising 1 DEG C with 2~3 hours (such as 2 hours) rises to barn temperature 54~55 DEG C (such as 55 DEG C), wet
Ball temperature, which slowly increases, is maintained at 37~40 DEG C (such as 38 DEG C), keeps blade dry and yellow is fixed up；In the dry muscle stage, with
1 DEG C of rate rises to barn temperature 67~69 DEG C (such as 69 DEG C) per hour, keeps wet-bulb temperature at 41~42 DEG C (such as 42 DEG C)
Left and right guarantees to cease fire after blade fixation is dry.
Smoking machine can be used in the suction.Cambridge filter can be used in the filtering.The cigarette and the coke tar in cigarette
Ratio concretely 20: 400mg.
In above-mentioned preparation method, the reagent can be methanol, water, ethyl acetate, ethyl alcohol, acetone, methylene chloride and chlorine
At least one of imitate.
In above-mentioned preparation method, the extraction can be ultrasonic wave extraction.
The condition of the ultrasonic wave extraction can be as follows: less than 70 DEG C of temperature (such as 15~30 DEG C, 25 DEG C), time 10min
~30min (such as 20min), power are 200~500W (such as 400W).
In above-mentioned preparation method, the method further includes being filtered simultaneously after the extraction to obtained extracting solution
The step of collecting filtrate.The filtering uses filter membrane.The filter membrane can be organic phase filter membrane or water phase filter membrane.The hole of the filter membrane
Diameter is less than 420 μm, concretely common 0.22 μm or 0.45 μm of laboratory.
Invention further provides the coke tar in cigarette extracts that above-mentioned preparation method is prepared.
Above-mentioned coke tar in cigarette extract as chemiluminescent substance or is preparing application in chemical luminous system,
Within the scope of the present invention.
The present invention also provides a kind of chemical luminous systems, it includes above-mentioned coke tar in cigarette extract.
In above-mentioned chemical luminous system, the mass concentration of the coke tar in cigarette extract can be 1.0mg/mL~8.0mg/
ML, concretely 4.0mg/mL.
Any one of the chemical luminous system is following A)-D):
It A) include the coke tar in cigarette extract and oxidant or oxidation system；
B it) is made of the coke tar in cigarette extract with oxidant or oxidation system；
It C) include the coke tar in cigarette extract and enzyme-H2O2System；
D) by the coke tar in cigarette extract and enzyme-H2O2System is constituted.
The oxidant can be H2O2、KIO4Any one of with NaClO；The oxidation system can be Fenton system
(Fe2+-H2O2System).Enzyme-the H2O2System can be HRP-H2O2System (horseradish peroxidase-H2O2System).
Specifically, the chemical luminous system A) can be following A 1)-A3) any one of:
It A1) include the coke tar in cigarette extract and H2O2；
It A2) include the coke tar in cigarette extract and KIO4Or NaClO；
It A3) include the coke tar in cigarette extract and Fenton system；
The chemical luminous system B) can be following B1)-B3) any one of:
B1) by the coke tar in cigarette extract and H2O2It constitutes；
B2) by the coke tar in cigarette extract and KIO4Or NaClO is constituted；
B3 it) is made of the coke tar in cigarette extract with Fenton system.
In above-mentioned chemical luminous system, including the coke tar in cigarette extract and H2O2Chemical luminous system or by institute
State coke tar in cigarette extract and H2O2In the chemical luminous system of composition, the mass concentration of the coke tar in cigarette extract can be
1.0mg/mL~8.0mg/mL, concretely 4.0mg/mL；H2O2Molar concentration can be 0.1mmol/L~1.0mol/L, specifically
It can be 1.0mmol/L.
In above-mentioned chemical luminous system, including the coke tar in cigarette extract and KIO4Or the chemical luminophor of NaClO
It is or by the coke tar in cigarette extract and KIO4Or in the chemical luminous system of NaClO composition, the coke tar in cigarette extract
Mass concentration can be 1.0mg/mL~8.0mg/mL, concretely 4.0mg/mL；KIO4Or the molar concentration of NaClO can be
0.1mmol/L~1.0mol/L, concretely 1.0mmol/L.
In above-mentioned chemical luminous system, the chemical luminous system including the coke tar in cigarette extract and Fenton system
Or in the chemical luminous system being made of the coke tar in cigarette extract and Fenton system, the matter of the coke tar in cigarette extract
Measuring concentration can be 1.0mg/mL~8.0mg/mL, concretely 4.0mg/mL；Fe in Fenton system2+Molar concentration can be 10
μm ol/L~0.01mol/L, concretely 0.1mmol/L；H2O2Molar concentration can be 0.1mmol/L~0.1mol/L, specifically
It can be 1.0mmol/L.
In above-mentioned chemical luminous system, including the coke tar in cigarette extract and enzyme-H2O2The chemical luminous system of system
Or by the coke tar in cigarette extract and enzyme-H2O2In the chemical luminous system that system is constituted, the matter of the coke tar in cigarette extract
Measuring concentration can be 1.0mg/mL~8.0mg/mL, concretely 4.0mg/mL；Enzyme-H2O2The mass concentration of enzyme can be in system
0.01mg/mL~0.1mg/mL, concretely 0.1mg/mL；H2O2Molar concentration can be 1.0mmol/L~0.1mol/L, tool
Body can be 1.0mmol/L.
Above-mentioned coke tar in cigarette extract, chemical luminophor tie up to detection application acid, in neutral or alkaline medium,
Within the scope of the present invention.
The invention has the following beneficial effects:
Coke tar in cigarette extract of the present invention be in unlimited system under the conditions of natural light as made from ultrasonic wave extraction,
Therefore the luminescent substance property for including is stablized, insensitive to factors such as illumination, oxygen and temperature；The extract is to PH values
Luminescence response range is wide, and chemiluminescence reaction can occur under acid, neutral or alkaline condition.Coke tar in cigarette of the present invention mentions
The raw material sources taken are extensive, and production method is simple.Coke tar in cigarette extract of the present invention is in chemiluminescence analysis detection technique field
Have wide practical use.
Detailed description of the invention
Fig. 1 is coke tar in cigarette extract and various concentration H in embodiment 12SO4With the chemiluminescence kinetic curve of NaOH,
Wherein Fig. 1 (a) is the H of various concentration2SO4, each label is expressed as follows: (1) 1.0mol/L, (2) 0.1mol/L, and (3) 1 × 10- 2Mol/L, (4) 1 × 10-3Mol/L, (5) 1 × 10-4Mol/L, (6) 1 × 10-5mol/L；Fig. 1 (b) is the NaOH of various concentration,
Each label is expressed as follows: (1) 1 × 10-5Mol/L, (2) 1 × 10-4Mol/L, (3) 1 × 10-3Mol/L, (4) 1 × 10-2Mol/L,
(5) 0.1mol/L, (6) 1.0mol/L.
Fig. 2 be in embodiment 1 coke tar in cigarette extract respectively under acid and alkaline condition with H2O2Chemiluminescence power
Curve is learned, wherein Fig. 2 (a) is the H of various concentration2SO4, each label is expressed as follows: (1) 1 × 10-5Mol/L, (2) 1 × 10- 4Mol/L, (3) 1 × 10-3Mol/L, (4) 1 × 10-2Mol/L (5) 0.1mol/L, (6) 1.0mol/L；Fig. 2 (b) is various concentration
NaOH, each label is expressed as follows: (1) 1 × 10-3Mol/L, (2) 1 × 10-2Mol/L, (3) 0.1mol/L, (4) 1.0mol/L.
Fig. 3 is the change of coke tar in cigarette extract and two kinds of common agents in acid, neutrality and alkaline medium in embodiment 1
Luminescence kinetics curve is learned, wherein the reagent of Fig. 3 (a) is KIO4, each label is expressed as follows: (1) 0.1mol/L H2SO4, (2)
H2O, (3) 1 × 10-5mol/L NaOH；The reagent of Fig. 3 (b) is NaClO, and each label is expressed as follows: (1) 0.1mol/L H2SO4,
(2)H2O, (3) 1 × 10-5mol/L NaOH。
Fig. 4 be embodiment 1 in coke tar in cigarette extract under different ph values with Fe2+-H2O2The chemistry of reagent reaction
Luminescence kinetics curve, each label are expressed as follows: (1) 1.0mol/L H2SO4, (2) 0.1mol/L H2SO4, (3) 0.01mol/L
H2SO4, (4) 1 × 10-3mol/L H2SO4, (5) 1 × 10-4mol/L H2SO4, (6) 1 × 10-5mol/L H2SO4, (7) 1 × 10- 6mol/L H2SO4, (8) pure water, (9) 1 × 10-6Mol/L NaOH, (10) 1 × 10-5Mol/L NaOH, (11) 1 × 10-4mol/L
NaOH, (12) 1 × 10-3Mol/L NaOH, (13) 0.01mol/L NaOH, (14) 0.1mol/L NaOH, (15) 1.0mol/L
NaOH。
Fig. 5 is coke tar in cigarette extract and HRP-H in embodiment 12O2System is in change acid, in neutral and alkaline medium
Luminescence kinetics curve is learned, each label is expressed as follows: (1) 1 × 10-4mol/L H2SO4, (2) H2O, (3) 1 × 10-5mol/L
NaOH。
Fig. 6 is the chemiluminescence of coke tar in cigarette extract (concentration 1.2mg/mL) and various concentration NaOH in embodiment 2
Kinetic curve, each label are expressed as follows: (1) (2) 1 × 10 0mol/L-6Mol/L, (3) 1 × 10-5Mol/L, (4) 1 × 10- 4Mol/L, (5) 1 × 10-3Mol/L, (6) 0.01mol/L, (7) 0.1mol/L, (8) 1.0mol/L.
Specific embodiment
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Cigarette as used in the following examples is what laboratory voluntarily rolled, the specific steps are as follows:
(1) baking modulation is carried out to tobacco leaf using " three-stage (flavescence, fixation and dry muscle three phases) " baking process, had
Steps are as follows for body: in yellowing stage, by the tobacco leaf binding of field acquisition, dress a heatable brick bed, with 1 DEG C per hour of rate by barn after igniting
Temperature rises to 38 DEG C, keeps wet-bulb temperature 1.5 DEG C lower than dry-bulb temperature, until 80% or more bottom canopy tobacco leaf changes to an eighty per cant yellow left side
Then barn temperature is increased to 42 DEG C by the right side, keep 37 DEG C of wet-bulb temperature, and appropriate hydrofuge, it is ensured that tobacco leaf reaches flavescence and softens
State；In the fixation stage, hydrofuge amount is increased, barn temperature is risen to 55 DEG C with the rate for rising 1 DEG C for 2 hours, wet-bulb temperature is slow
Raising is maintained at 38 DEG C, keeps blade dry and yellow is fixed up；In the dry muscle stage, with 1 DEG C per hour of rate by barn
Temperature rises to 69 DEG C, keeps wet-bulb temperature at 42 DEG C or so, guarantees to cease fire after blade fixation is dry；
(2) modulated fine earth, sand grains and dust of the tobacco leaf on fur brush outwash blade will be toasted in step (1),
It gets damp again 10 hours at room temperature, removes chopping after tobacco leaf main reinforcement, it is rolled after chopping, it is ensured that every cigarette length
For 70mm, circumference 27.5mm, weight is 1.1 ± 0.1g；The tar content of every cigarette is 20mg.
The manufacturer of smoking machine as used in the following examples be Borgwaldt company, Germany, model RM20/cs,
The manufacturer of cambridge filter is Borgwaldt company, Germany, model 8,020 284 1；The manufacturer of organic phase filter membrane is
Town in Shanghai spectrum experiment Science and Technology Co., Ltd., model SCAA-103.The manufacturer of water phase filter membrane is Town in Shanghai spectrum experiment
Science and Technology Co., Ltd., model SCAA-101.
Luminescence kinetics curve in following embodiments be in Weak light investigating instrument (BPCL-GP15-TGC) by quiet
What the method for state injection obtained, negative high voltage is -1000V.
The preparation method of the coke tar in cigarette extract with chemiluminescence property in following embodiments, such as without special theory
It is bright, unlimited system, (25 DEG C) progress under room temperature under the conditions of natural light.
Embodiment 1, preparation have chemiluminescence property coke tar in cigarette extract (reagent is methanol)
One, preparation method
Coke tar in cigarette extract is prepared in accordance with the following steps:
20 cigarette are once aspirated using smoking machine, and the flue gas that suction obtains is retained into flue gas therein by cambridge filter
Granule phase substance obtains the cambridge filter that load has coke tar in cigarette；After cambridge filter be cut into strip be added in 40mL methanol
It carries out ultrasonic wave extraction 20min (ultrasonic power: 400W), crosses 0.45 μm of organic phase filter membrane, finally collect filtrate and obtain cigarette
Tar extract.
Two, chemiluminescence property
1, chemiluminescence phenomenon of the coke tar in cigarette extract to strong acid and highly basic
The above-mentioned coke tar in cigarette extract being prepared is measured respectively in the H of various concentration2SO4With the chemistry hair in NaOH
Photodynamics curve (mass concentration of coke tar in cigarette extract is 4.0mg/mL), experimental result is as shown in Figure 1.It can by Fig. 1 (a)
It is 1 × 10 in sulfuric acid concentration to find out-5In the range of mol/L~1.0mol/L, tar extract of the present invention has chemiluminescence to ring
It answers.It can be seen from Fig. 1 (b) in strongly basic medium, when naoh concentration is 0.1mol/L, tar extract has chemistry
Luminescence response, the luminous intensity when luminous intensity that naoh concentration generates when being 1.0mol/L is much larger than 0.1mol/L.Cause
This, tar extract of the present invention has chemiluminescence response in strong acid and highly basic medium.
2, under acid and alkaline condition with H2O2Chemiluminescence phenomenon
The above-mentioned coke tar in cigarette extract and H being prepared is measured respectively2O2Chemical luminous system (the coke tar in cigarette of composition
The mass concentration of extract is 4.0mg/mL, H2O2Molar concentration be 1.0mmol/L) in the H of various concentration2SO4In NaOH
Chemiluminescence kinetic curve, experimental result is as shown in Figure 2.When sulfuric acid concentration is 1 × 10 it can be seen from Fig. 2 (a)- 2When mol/L~1.0mol/L range, tar extract of the present invention and hydrogen peroxide have apparent chemiluminescence signal；By Fig. 2
(b) as can be seen that in alkaline medium, when naoh concentration is respectively 1 × 10-5When mol/L and 1.0mol/L, the present invention is burnt
Oil extract and hydrogen peroxide have apparent luminous signal.The experiment shows tar extract of the present invention in acid and alkaline environment
In can and H2O2Chemiluminescence reaction occurs.Therefore, tar extract of the present invention can be based on tar extract-H2O2It establishes
Chemical luminous system is for the substance detection in acid and alkaline medium.
3, coke tar in cigarette extract and KIO4Or NaClO is in chemiluminescence phenomenon acid, in neutral and alkaline medium
The above-mentioned coke tar in cigarette extract and KIO being prepared is measured respectively4Or the chemical luminous system that NaClO is constituted
(mass concentration of coke tar in cigarette extract is 4.0mg/mL；KIO4Molar concentration be 1.0mmol/L, the molar concentration of NaClO
It is 1.0mmol/L) in H2SO4、H2Chemiluminescence kinetic curve in O and NaOH, experimental result are as shown in Figure 3.It can by Fig. 3
To find out, two kinds of reagents are respectively in H2SO4(0.1mol/L)、H2O and NaOH (1 × 10-5Mol/L) in medium with tar extract
Chemiluminescence phenomenon can be generated.Therefore, the present invention is based on the luminescence systems of tar extract and both the above reaction reagent
Also there is potential using value in terms of analysis detection.
4, tar extract under different ph values with the chemiluminescence phenomenon of Fenton system
Chemical luminous system (the volume of the above-mentioned coke tar in cigarette extract being prepared and Fenton system composition is measured respectively
The mass concentration of smoke tar extract is 4.0mg/mL；Fe2+-H2O2Fe in system2+Molar concentration be 0.1mmol/L, H2O2's
Molar concentration is 1.0mmol/L), chemiluminescence intensity various concentration H2SO4It is bent with the chemiluminescence dynamics in NaOH
Line, experimental result are as shown in Figure 4.As seen from Figure 4, in 0~14 range of PH values, tar extract of the present invention with
Fe2+-H2O2System has chemiluminescence signal, shows tar extract of the present invention equal energy under acid, neutral and alkaline condition
Chemiluminescence phenomenon is generated with Fenton system, and hydroxyl radical free radical is chief active species in Fenton system, therefore hydroxyl
Base free radical may be the reaction species for causing tar extract luminous.
5, enzyme (HRP)-H2O2System and tar extract are in chemiluminescence phenomenon acid, under neutral and alkaline condition
The above-mentioned coke tar in cigarette extract being prepared and enzyme (HRP)-H are measured respectively2O2The chemical luminophor that system is constituted
(mass concentration of coke tar in cigarette extract is 4.0mg/mL for system；Enzyme (HRP)-H2O2The mass concentration of HRP is 0.1mg/ in system
ML, H2O2Molar concentration be 1.0mmol/L) chemiluminescence intensity in H2SO4、H2Chemiluminescence dynamics in O and NaOH
Curve, experimental result are as shown in Figure 5.As seen from Figure 5, tar extract and HRP-H of the present invention2O2System is in H2SO4(1×
10-4mol/L)、H2O and NaOH (1 × 10-5Mol/L chemiluminescence phenomenon) can be generated in medium, therefore the present invention can be based on
Tar extract-HRP-H2O2Luminescence system is established for analysis detection acid, in neutral and alkaline medium.
Embodiment 2, preparation have chemiluminescence property coke tar in cigarette extract (reagent is water)
One, preparation method
Coke tar in cigarette extract is prepared in accordance with the following steps:
20 cigarette are once aspirated using smoking machine, and the flue gas that suction obtains is retained into flue gas therein by cambridge filter
Granule phase substance obtains the cambridge filter that load has coke tar in cigarette；After by cambridge filter be cut into strip be added in 40mL water into
Row ultrasonic wave extraction 20min (ultrasonic power: 400W) crosses 0.45 μm of water phase filter membrane, finally collects filtrate and obtains coke tar in cigarette
Extract.
Two, chemiluminescence property
1, chemiluminescence phenomenon of the coke tar in cigarette extract to highly basic
It is bent to measure chemiluminescence dynamics of the above-mentioned coke tar in cigarette extract being prepared in the NaOH of various concentration
Line (mass concentration of coke tar in cigarette extract is 1.2mg/mL), experimental result is as shown in Figure 6.As seen from Figure 6, in highly basic
Property medium in, naoh concentration be 0.01mol/L~1.0mol/L when, tar extract has chemiluminescence response.
Claims (10)
1. a kind of preparation method of coke tar in cigarette extract includes the following steps: to extract coke tar in cigarette addition reagent, obtain
To coke tar in cigarette extract.
2. preparation method according to claim 1, it is characterised in that: the ratio of the coke tar in cigarette and the reagent is
400mg:(20~150) mL；And/or
The reagent is at least one of methanol, water, ethyl acetate, ethyl alcohol, acetone, methylene chloride and chloroform.
3. preparation method according to claim 1 or 2, it is characterised in that: described to be extracted as ultrasonic wave extraction；And/or
The condition of the ultrasonic wave extraction is as follows: temperature be less than 70 DEG C, the time be 10min~30min, power be 200~
500W。
4. preparation method according to any one of claim 1-3, it is characterised in that: the method is after the extraction
Further include the steps that being filtered obtained extracting solution and collects filtrate；And/or
The filtering uses filter membrane；And/or
The aperture of the filter membrane is less than 420 μm.
5. the coke tar in cigarette extract that preparation method of any of claims 1-4 is prepared.
6. coke tar in cigarette extract described in claim 5 as chemiluminescent substance or is being prepared in chemical luminous system
Using.
7. a kind of chemical luminous system, it is characterised in that: it includes coke tar in cigarette extract described in claim 5.
8. chemical luminous system according to claim 7, it is characterised in that: the chemical luminous system is following A)-D)
Any one of:
It A) include the coke tar in cigarette extract and oxidant or oxidation system；
B it) is made of the coke tar in cigarette extract with oxidant or oxidation system；
It C) include the coke tar in cigarette extract and enzyme-H2O2System；
D) by the coke tar in cigarette extract and enzyme-H2O2System is constituted.
9. chemical luminous system according to claim 8, it is characterised in that: the oxidant is H2O2、KIO4And NaClO
Any one of；The oxidation system is Fenton system；Enzyme-the H2O2System is HRP-H2O2System.
10. chemical luminophor described in any one of coke tar in cigarette extract, claim 7-9 described in claim 5 ties up to
Detection application acid, in neutral or alkaline medium.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111035055A (en) * | 2019-11-27 | 2020-04-21 | 内蒙古昆明卷烟有限责任公司 | Method for extracting tar aroma components in cigarette butts and application of tar aroma components in cigarettes |
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| WO2021104082A1 (en) | 2019-11-27 | 2021-06-03 | 内蒙古昆明卷烟有限责任公司 | Method for preparing tar extract having aromatic characteristics by using discarded cigarette butt, and use thereof in cigarette |
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| KR20220124689A (en) | 2019-11-27 | 2022-09-14 | 이너 몽골리아 쿤밍 시가렛 리미티드 라이어빌리티 컴퍼니 | Method for preparing tar extract from waste cigarette butts and application thereof in cigarettes |
| KR20220124690A (en) | 2019-11-27 | 2022-09-14 | 이너 몽골리아 쿤밍 시가렛 리미티드 라이어빌리티 컴퍼니 | Method for extracting tar odor-generating component from cigarette butts and application thereof in cigarette |
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| US11839232B2 (en) | 2019-11-27 | 2023-12-12 | Inner Mongolia Kunming Cigarette Co., Ltd. | Method for preparing tar extract having aromatic characteristics by using discarded cigarette butt, and use thereof in cigarette |
| US12004553B2 (en) | 2019-11-27 | 2024-06-11 | Inner Mongolia Kunming Cigarette Co., Ltd. | Method for extracting tar aroma components from within cigarette butts and application thereof in cigarettes |
| CN111141727A (en) * | 2020-01-08 | 2020-05-12 | 中国农业科学院烟草研究所 | Method for evaluating catalytic oxidation performance of catalytic material |
| CN113504221A (en) * | 2021-07-06 | 2021-10-15 | 中国农业科学院烟草研究所 | Application of tea extract in sulfate radical free radical detection |
| CN113504221B (en) * | 2021-07-06 | 2023-12-08 | 中国农业科学院烟草研究所 | Application of tea extract in sulfate radical detection |
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