CN109929751B - Fluorescent quantitative amplification detector - Google Patents
Fluorescent quantitative amplification detector Download PDFInfo
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- CN109929751B CN109929751B CN201910325984.3A CN201910325984A CN109929751B CN 109929751 B CN109929751 B CN 109929751B CN 201910325984 A CN201910325984 A CN 201910325984A CN 109929751 B CN109929751 B CN 109929751B
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- 230000003321 amplification Effects 0.000 title claims abstract description 31
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 31
- 238000001514 detection method Methods 0.000 claims abstract description 47
- 230000005284 excitation Effects 0.000 claims abstract description 30
- 238000010438 heat treatment Methods 0.000 claims abstract description 27
- 238000001917 fluorescence detection Methods 0.000 claims abstract description 14
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 14
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 14
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 14
- 238000001816 cooling Methods 0.000 claims abstract description 13
- 230000017525 heat dissipation Effects 0.000 claims abstract description 12
- 239000011159 matrix material Substances 0.000 claims abstract description 4
- 239000000126 substance Substances 0.000 claims description 3
- 229910052736 halogen Inorganic materials 0.000 claims description 2
- 150000002367 halogens Chemical class 0.000 claims description 2
- 238000004321 preservation Methods 0.000 claims description 2
- 229910052724 xenon Inorganic materials 0.000 claims description 2
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 claims description 2
- 230000035945 sensitivity Effects 0.000 abstract description 3
- 238000010586 diagram Methods 0.000 description 5
- 239000013307 optical fiber Substances 0.000 description 4
- 230000005540 biological transmission Effects 0.000 description 3
- 238000003753 real-time PCR Methods 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000004544 DNA amplification Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 230000008054 signal transmission Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention relates to a fluorescent quantitative amplification detector which comprises a shell, a nucleic acid amplification module arranged in the shell, an XY axis linear module arranged at the bottom of the shell, at least one fluorescent detection module arranged at the output end of the XY axis linear module, and a control panel arranged at the front of the shell and used for controlling the working states of the nucleic acid amplification module, the fluorescent detection module and the XY axis linear module and displaying detection data; the nucleic acid amplification module comprises a heating frame, a plurality of sample through holes, thermoelectric cooling plates and a heat dissipation assembly, wherein the heating frame is arranged above the fluorescent detection module and is exposed out of the upper end of the shell, the sample through holes are arranged on the heating frame in a matrix arrangement, and the thermoelectric cooling plates and the heat dissipation assembly are respectively arranged on one side of the heating frame and are placed from inside to outside; according to the invention, the XY axis linear module can drive the fluorescence detection module to move to the position right below the sample tube, so that the fluorescence detection is directly carried out on the bottom of the sample tube, the excitation light efficiency is improved, the higher fluorescence sensitivity and fluorescence signal intensity are obtained, and the detection result is more accurate.
Description
Technical Field
The invention relates to the field of fluorescence detection, in particular to a fluorescent quantitative amplification detector.
Background
The nucleic acid amplification fluorescent detection system is a system for realizing qualitative and quantitative detection of a sample by irradiating excitation light to a reagent in a sample tube and then receiving fluorescence instantaneously excited from the reagent in the sample tube.
At present, some fluorescence quantitative detection devices are also in the market, such as a fluorescence quantitative PCR detector optical excitation and detection system is disclosed in the prior art 201410199206.1, a rapid and multichannel real-time fluorescence quantitative detection device is disclosed in the prior art 201510031523.7, a real-time fluorescence quantitative PCR gene amplification detector is disclosed in the prior art 201710286911.9, the top detection modes adopted by the detection devices are that excitation light enters a sample tube from the top of the sample tube through light transmission refraction of a top cover, and generated fluorescence signals are reflected in the sample tube and then returned to be received through the top cover of the sample tube, but the detection modes have high requirements on the light transmission performance of the top cover of the sample tube, seriously influence the sensitivity of sample detection, and when the distance is far, the light emission is weakened and the signal is weakened; in addition, as disclosed in prior art 201610152466.2, a multi-fluorescent channel detection system for real-time fluorescent quantitative PCR is disclosed in prior art 201711310372.4, and a side detection mode is disclosed in which excitation light enters the sample tube from the side of the sample tube through an optical fiber, and the generated fluorescent signal is reflected in the sample tube and then received by the same or another optical fiber on the side of the sample tube, and although the requirement on the light transmission performance of the top cover of the sample tube is not high in the detection mode, the optical fiber is very difficult to embed and maintain.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a fluorescent quantitative amplification detector.
In order to achieve the above purpose, the invention adopts the following technical scheme: a fluorescent quantitative amplification detector comprises a shell, a nucleic acid amplification module arranged in the shell, an XY axis linear module arranged at the bottom of the shell, at least one fluorescent detection module arranged at the output end of the XY axis linear module, and a control panel arranged on the front surface of the shell and used for controlling the working states of the nucleic acid amplification module, the fluorescent detection module and the XY axis linear module and displaying detection data; the nucleic acid amplification module comprises a heating frame, a plurality of sample through holes, thermoelectric cooling plates and a heat dissipation assembly, wherein the heating frame is arranged above the fluorescent detection module and is exposed out of the upper end of the shell, the sample through holes are arranged on the heating frame in a matrix arrangement and are used for placing sample tubes, and the thermoelectric cooling plates and the heat dissipation assembly are respectively arranged on one side of the heating frame and are placed from inside to outside; the upper end of the shell is also respectively provided with a shell cover capable of outwards overturning and a spring lock catch for fixing the shell cover.
Preferably, the fluorescence detection module comprises a box body, a light source, an excitation filter, a dichroic mirror, a detection filter, a fluorescence detector and a reflecting prism which are respectively arranged in the box body; the light source emits horizontal light to generate excitation light with corresponding wavelength through the excitation filter, the bicolor mirror is obliquely placed at 45 degrees with the excitation light, the excitation light is horizontally reflected to the reflecting prism, the reflecting prism reflects the horizontal excitation light and then vertically irradiates the bottom of the sample tube, fluorescent substances in the sample tube are excited to generate fluorescence, part of the fluorescence is horizontally reflected to the bicolor mirror through the reflecting prism, then the fluorescence is incident to the detection filter through the bicolor mirror to filter out pure fluorescence, and finally the filtered fluorescence is irradiated to the fluorescence detector; lenses are arranged in the box body between the light source and the excitation filter, between the detection filter and the fluorescence detector and between the reflecting prism and the sample tube.
Preferably, the thermoelectric cooling fin and the heat dissipation component are both arranged at two and are respectively arranged at one side of the long side of the heating frame from inside to outside.
Preferably, a groove is formed in the upper end of the shell; the shell cover can be outwards overturned and arranged in the groove; the heating frame is exposed out of the bottom surface of the groove; and the shell cover is also provided with a heat preservation cover with a shape corresponding to the heating frame.
Preferably, the front side of the housing is inclined upward.
Preferably, the inclination angle of the front surface of the shell is 45 degrees.
Preferably, the two side surfaces of the shell are also respectively provided with a radiating window.
Preferably, the light source is one of an LED lamp, a xenon lamp, a halogen lamp or a laser light source.
Preferably, the fluorescence detector is one of a CCD detector, a photodiode, or a PMT photomultiplier.
Due to the application of the technical scheme, compared with the prior art, the invention has the following advantages:
1. the invention can drive the fluorescent detection module to move to the position right below the sample tube through the XY axis linear module, directly carries out fluorescent detection on the bottom of the sample tube, does not need optical fibers for signal transmission, is beneficial to improving the excitation light efficiency, obtains higher fluorescence sensitivity and fluorescence signal intensity, ensures more accurate detection result, and can greatly reduce the production and maintenance cost;
2. according to the fluorescence detection module, the reflection prism is added, so that the horizontal excitation light can be reflected and vertically emitted into the bottom of the sample tube, the thickness of the box body can be greatly reduced, and the whole volume is reduced;
3. According to the invention, the thermoelectric cooling fin and the heat dissipation component are both arranged, so that the temperature can be quickly increased or decreased, the detection time is shortened, and the detection efficiency is improved.
Drawings
The technical scheme of the invention is further described below with reference to the accompanying drawings:
FIG. 1 is a schematic structural diagram of a fluorescent quantitative amplification detector according to the present invention;
FIG. 2 is a schematic view of the invention with the housing removed;
FIG. 3 is a schematic diagram of a fluorescent detection module mounted on an XY axis linear module according to the present invention;
FIG. 4 is a schematic diagram of a fluorescence detection module according to the present invention;
FIG. 5 is a circuit diagram of a fluorescent quantitative amplification detector according to the present invention.
Wherein: 1. a control panel; 2. a housing; 3. a sample tube; 4. a groove; 5. a thermal insulation cover; 6. a cover; 7. a heat radiation window; 8. a nucleic acid amplification module; 81. a heating rack; 82. a sample through hole; 83. thermoelectric cooling sheets; 84. a heat dissipation assembly; 9. an XY axis straight line module; 10. a spring lock catch; 11. a fluorescence detection module; 111. a case body; 112. an LED lamp; 113. a lens; 114. an excitation filter; 115. a reflecting prism; 116. a dichroic mirror; 117. a detection filter; 118. a CCD detector.
Detailed Description
The invention will be described in further detail with reference to the accompanying drawings and specific examples.
Fig. 1-5 are diagrams showing a fluorescence quantitative amplification detector according to the present invention, including a housing 2, a nucleic acid amplification module 8 disposed in the housing 2, an XY axis linear module 9 disposed at the bottom of the housing 2, two fluorescence detection modules 11 disposed side by side at the output end of the XY axis linear module 9 and having different wavelengths, and a control panel 1 disposed on the front surface of the housing 2 for controlling the working states of the nucleic acid amplification module 8, the fluorescence detection modules 11 and the XY axis linear module 9, and displaying detection data, respectively; the nucleic acid amplification module 8 comprises a heating frame 81 arranged above the fluorescence detection module 11 and exposing the upper end of the shell 2, a plurality of sample through holes 82 which are arranged on the heating frame 81 in a matrix and used for placing the sample tubes 3, thermoelectric cooling fins 83 and a heat dissipation component 84 which are respectively arranged on one side of the heating frame 81 and are placed from inside to outside; the upper end of the shell 2 is also respectively provided with a shell cover 6 which can be turned outwards and a spring lock catch 10 used for fixing the shell cover 6; when the fluorescent detection device is used, the sample tube 3 is inserted into the sample through hole 82 on the heating frame 81, the shell cover 6 is covered and fixed through the spring lock catch 10, then the fluorescent detection module 11 with required wavelength can be driven by the XY axis linear module 9 to move to the position right below the sample tube 3 under the control of the control panel 1, the bottom of the sample tube 3 is directly subjected to fluorescent detection, and finally detection data is uploaded to the control panel 1 for display.
Further, the fluorescence detection module 11 includes a case, an LED lamp 112, an excitation filter 114, a dichroic mirror 116, a detection filter 117, a CCD detector 118, and a reflection prism 115, which are respectively disposed in the case; the LED lamp 112 emits horizontal light to generate excitation light with a corresponding wavelength through the excitation filter 114, the dichroic mirror 116 is placed at an angle of 45 degrees with the excitation light, the excitation light is horizontally reflected to the reflecting prism 115, the reflecting prism 115 reflects the horizontal excitation light and then vertically irradiates the bottom of the sample tube 3, fluorescent substances in the sample tube 3 are excited to generate fluorescence, part of the fluorescence is horizontally reflected to the dichroic mirror 116 through the reflecting prism 115, then the fluorescence is incident to the detection filter 117 through the dichroic mirror 116 to filter out pure fluorescence, and finally the filtered fluorescence is irradiated to the CCD detector 118; the inside of the box body is provided with lenses 113 between the LED lamp 112 and the excitation filter 114, between the detection filter 117 and the CCD detector 118 and between the reflecting prism 115 and the sample tube 3, and the light can be converged through the lenses 113, so that the measurement is more accurate.
Further, the thermoelectric cooling fins 83 and the heat dissipation component 84 are both provided with two, and are respectively placed on one side of the long side of the heating frame 81 from inside to outside, so that the temperature can be quickly raised or lowered, thereby shortening the detection time and improving the detection efficiency.
Further, a groove 4 is formed at the upper end of the shell 2; the shell cover 6 can be outwards overturned and is arranged in the groove 4; the heating frame 81 exposes the bottom surface of the groove 4; the cover 6 is also provided with a heat-insulating cover 5 with a shape corresponding to the heating frame 81, so that the heating or cooling speed is further improved.
Further, the casing 2 is disposed with its front side inclined at 45 degrees upward, so that the user can operate the control panel 1 or read data from the control panel 1.
Further, the two side surfaces of the shell are also respectively provided with a radiating window 7, so that the radiating effect is enhanced.
The foregoing is merely a specific application example of the present invention, and the protection scope of the present invention is not limited in any way. All technical schemes formed by equivalent transformation or equivalent substitution fall within the protection scope of the invention.
Claims (8)
1. A fluorescent quantitative amplification detector is characterized in that: the device comprises a shell, a nucleic acid amplification module arranged in the shell, an XY axis linear module arranged at the bottom of the shell, at least one fluorescence detection module arranged at the output end of the XY axis linear module, and a control panel arranged on the front surface of the shell and used for controlling the working states of the nucleic acid amplification module, the fluorescence detection module and the XY axis linear module and displaying detection data respectively;
the nucleic acid amplification module comprises a heating frame, a plurality of sample through holes, thermoelectric cooling plates and a heat dissipation assembly, wherein the heating frame is arranged above the fluorescent detection module and is exposed out of the upper end of the shell, the sample through holes are arranged on the heating frame in a matrix arrangement and are used for placing sample tubes, and the thermoelectric cooling plates and the heat dissipation assembly are respectively arranged on one side of the heating frame and are placed from inside to outside;
The upper end of the shell is also provided with a shell cover capable of outwards overturning and a spring lock catch for fixing the shell cover;
the fluorescence detection module comprises a box body, a light source, an excitation filter, a dichroic mirror, a detection filter, a fluorescence detector and a reflecting prism, wherein the light source, the excitation filter, the dichroic mirror, the detection filter, the fluorescence detector and the reflecting prism are respectively arranged in the box body; the light source emits horizontal light to generate excitation light with corresponding wavelength through the excitation filter, the bicolor mirror is obliquely placed at 45 degrees with the excitation light, the excitation light is horizontally reflected to the reflecting prism, the reflecting prism reflects the horizontal excitation light and then vertically irradiates the bottom of the sample tube, fluorescent substances in the sample tube are excited to generate fluorescence, part of the fluorescence is horizontally reflected to the bicolor mirror through the reflecting prism, then the fluorescence is incident to the detection filter through the bicolor mirror to filter out pure fluorescence, and finally the filtered fluorescence is irradiated to the fluorescence detector; lenses are arranged in the box body between the light source and the excitation filter, between the detection filter and the fluorescence detector and between the reflecting prism and the sample tube.
2. The fluorescent quantitative amplification detector of claim 1, wherein: the thermoelectric cooling plates and the heat dissipation components are arranged in two and are respectively arranged on one side of the long side of the heating frame from inside to outside.
3. The fluorescent quantitative amplification detector of claim 2, wherein: the upper end of the shell is provided with a groove; the shell cover can be outwards overturned and arranged in the groove; the heating frame is exposed out of the bottom surface of the groove; and the shell cover is also provided with a heat preservation cover with a shape corresponding to the heating frame.
4. The fluorescent quantitative amplification detector of claim 3, wherein: the right side of the shell is obliquely arranged upwards.
5. The fluorescent quantitative amplification detector of claim 4, wherein: the inclination angle of the front surface of the shell is 45 degrees.
6. The fluorescent quantitative amplification detector of claim 5, wherein: and the two side surfaces of the shell are also respectively provided with a radiating window.
7. The fluorescent quantitative amplification detector of claim 6, wherein: the light source is one of an LED lamp, a xenon lamp, a halogen lamp or a laser light source.
8. The fluorescent quantitative amplification detector of claim 7, wherein: the fluorescence detector is one of a CCD detector, a photodiode or a PMT photomultiplier.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910325984.3A CN109929751B (en) | 2019-04-23 | 2019-04-23 | Fluorescent quantitative amplification detector |
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| CN201910325984.3A CN109929751B (en) | 2019-04-23 | 2019-04-23 | Fluorescent quantitative amplification detector |
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| CN109929751A CN109929751A (en) | 2019-06-25 |
| CN109929751B true CN109929751B (en) | 2024-07-23 |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111286444A (en) * | 2020-02-27 | 2020-06-16 | 中国科学院上海技术物理研究所 | High-sensitivity virus rapid detector |
| CN112683869B (en) * | 2020-12-25 | 2023-03-14 | 中国科学院苏州生物医学工程技术研究所 | Fluorescent quantitative detection method |
| CN113337382A (en) * | 2021-05-06 | 2021-09-03 | 北京谊安和景生物科技有限公司 | High-flux nucleic acid detection system |
| CN113981139B (en) * | 2021-09-27 | 2022-11-25 | 厦门健康工程与创新研究院 | Nucleic acid isothermal amplification detection reagent, kit, detection system and method |
| CN114047170B (en) * | 2021-11-27 | 2022-08-16 | 广州普世君安生物科技有限公司 | Constant temperature fluorescence detector and multichannel fluorescence detection structure |
| CN114705665A (en) * | 2022-06-02 | 2022-07-05 | 圣湘生物科技股份有限公司 | Fluorescence detection device and fluorescence detection method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2482080Y (en) * | 2001-04-12 | 2002-03-13 | 杭州大和热磁电子有限公司 | Fluorescent quantitative polymerase chain reaction diagnostic apparatus |
| CN209989394U (en) * | 2019-04-23 | 2020-01-24 | 苏州合惠生物科技有限公司 | Novel fluorescence quantitative amplification detector |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5216318B2 (en) * | 2007-12-27 | 2013-06-19 | 株式会社日立ハイテクノロジーズ | Fluorescence detection device |
| CN106290267A (en) * | 2015-05-18 | 2017-01-04 | 北京怡成生物电子技术股份有限公司 | Fluorescence detection device |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2482080Y (en) * | 2001-04-12 | 2002-03-13 | 杭州大和热磁电子有限公司 | Fluorescent quantitative polymerase chain reaction diagnostic apparatus |
| CN209989394U (en) * | 2019-04-23 | 2020-01-24 | 苏州合惠生物科技有限公司 | Novel fluorescence quantitative amplification detector |
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