CN109924506B - Probiotic composition with weight-losing function and preparation method and application thereof - Google Patents
Probiotic composition with weight-losing function and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a probiotic composition with a weight-losing function and a preparation method and application thereof. The lactobacillus paracasei is preserved in China center for type culture Collection with the preservation numbers as follows: CCTCC NO: and M2018302. The embodiment of the invention provides a probiotic composition with a weight-losing function, which takes lactobacillus paracasei bacterial powder and lactobacillus paracasei metabolite powder as active ingredients. The composition has effects of reducing weight, reducing blood lipid, and enhancing immunity.
Description
Technical Field
The invention relates to the technical field of microbial preparations, in particular to a probiotic composition with functions of reducing fat and losing weight and a preparation method and application thereof.
Background
With the improvement of living standard, more and more people are added to the ranks of obese people, and the obese people are more likely to suffer from diseases such as diabetes, hypertension and the like. Therefore, weight-losing medicines are needed to assist weight-losing in many cases, so that over-obesity is avoided. At present, the weight-reducing medicines and health-care varieties on the market are various, such as weight-reducing granules, weight-reducing tablets, weight-reducing tea and the like. Although the weight-losing tea has a certain weight-losing effect, the tea needs to be matched with diet control, and has certain side effects, and after the tea is stopped being used, the appetite rebounds and the body weight is increased, so that the ideal weight-losing effect cannot be achieved, and further improvement is urgently needed.
Disclosure of Invention
The embodiment of the invention aims to provide a probiotic composition with a weight-losing function and a preparation method and application thereof, and aims to overcome the defects that the existing weight-losing medicine has side effects, the weight-losing effect is not ideal, and weight losing is easy to rebound.
In order to achieve the above object, the embodiment of the present invention provides a probiotic composition with a weight-reducing function, which uses lactobacillus paracasei bacterial powder and lactobacillus paracasei metabolite powder as active ingredients.
Preferably, the lactobacillus paracasei is preserved in the China center for type culture Collection with the preservation numbers: CCTCC NO: and M2018302.
Preferably, the composition also comprises a fungus powder carrier, wherein the fungus powder carrier comprises microcrystalline cellulose, medicinal corn starch, inulin and magnesium stearate;
the composition comprises the following raw materials in parts by mass: 9-11 parts of lactobacillus paracasei powder, 4-6 parts of lactobacillus paracasei metabolite powder, 18-22 parts of microcrystalline cellulose, 80-120 parts of medicinal corn starch, 8-12 parts of inulin and 8-12 parts of magnesium stearate.
Preferably, the composition comprises the following raw materials in parts by mass: 9.5-10.5 parts of lactobacillus paracasei powder, 4.5-5.5 parts of lactobacillus paracasei metabolite powder, 19-21 parts of microcrystalline cellulose, 90-110 parts of medicinal corn starch, 9-11 parts of inulin and 9-11 parts of magnesium stearate.
The embodiment of the invention also provides a method for preparing the composition, which comprises the steps of carrying out expanded strain culture, primary seed culture, seed tank culture and fermentation culture on the lactobacillus paracasei to obtain lactobacillus paracasei zymocyte liquid;
centrifuging the lactobacillus paracasei fermented liquid to respectively collect lactobacillus paracasei bacterial sludge and centrifuged fermentation liquid, and respectively freezing, drying, crushing, concentrating, drying and crushing the lactobacillus paracasei bacterial sludge and the centrifuged fermentation liquid to obtain lactobacillus paracasei bacterial powder and lactobacillus paracasei metabolite powder;
and mixing the lactobacillus paracasei bacterial powder, the lactobacillus paracasei metabolite powder and a bacterial powder carrier to obtain the composition.
Preferably, the preparation method of the lactobacillus paracasei powder comprises the following steps:
and mixing the lactobacillus paracasei bacterial sludge, sterile water, skim milk powder, fructo-oligosaccharide, glycerol, maltodextrin and isomalto-oligosaccharide to obtain lactobacillus paracasei suspended bacterial liquid, and freeze-drying and crushing the suspended bacterial liquid to obtain lactobacillus paracasei bacterial powder.
Preferably, the suspension liquid is pre-frozen for 2 to 5 hours at the temperature of between 35 ℃ below zero and 30 ℃ below zero, and is subjected to vacuum freeze drying at the temperature of between 50 ℃ below zero and 40 ℃ below zero, and the suspension liquid is crushed into 100-mesh lactobacillus paracasei powder.
Preferably, the preparation method of the lactobacillus paracasei metabolite powder comprises the following steps:
concentrating the centrifuged fermentation liquor at low temperature to obtain a concentrated fermentation liquor paste with the water content of at most 20%, mixing the concentrated fermentation liquor paste with medicinal corn starch in a ratio of 5: 1-4: 1 to obtain a mixture, then drying the mixture for 40 to 50 hours at the temperature of 55 to 60 ℃, and crushing the mixture to obtain the lactobacillus paracasei metabolite powder with the water content of at most 3 percent.
The embodiment of the invention also provides the application of the composition prepared by the method in preparing a product with at least one of the following functions:
1) losing weight;
2) promoting metabolism;
3) reducing blood fat;
4) enhancing immunity.
The embodiment of the invention has the following advantages:
the embodiment of the invention provides a probiotic composition with a weight-losing function, which takes lactobacillus paracasei bacterial powder and lactobacillus paracasei metabolite powder as active ingredients. The lactobacillus paracasei powder in the composition has the advantages of obvious weight-losing effect, good blood fat-reducing effect and immunity-enhancing effect.
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
The embodiment of the invention provides a probiotic composition with a weight-losing function, which takes lactobacillus paracasei bacterial powder and lactobacillus paracasei metabolite powder as active ingredients. The Lactobacillus paracasei is separated from fresh milk, is screened primarily and screened again, is a strain with the weight-losing effect, is identified as Lactobacillus paracasei +18(Lactobacillus paracasei +18) through 16S DNA, has a sequence shown as SEQ ID 1, is subjected to patent program biological preservation, is preserved in China center for type culture collection, and has the preservation date as follows: 21/05/2018, the preservation address is as follows: the Wuhan city Wuchang Lojia mountain has the preservation number: CCTCC NO: and M2018302.
Example 1
Expanding culture of paracasei lactobacillus
The strain of the embodiment of the invention adopts lactobacillus paracasei, and the equipment used in the production is as follows: a strain incubator, an ultra-clean workbench, a constant-temperature incubator, various test tubes, a triangular flask, a seeding tank, a fermentation tank, a vacuum freeze dryer, a three-dimensional stirrer, a low-temperature crusher and a vacuum packaging machine.
And (3) strain culture: enlarged culture of-80 ℃ preservation strain, preparation of enlarged strain culture medium: 16 g of glucose, 10 g of peptone, 8 g of beef extract powder, 2g of sodium acetate, 801 ml of tween, 1 g of urea and 1000 ml of tap water, and the pH value is natural. Adding glucose and other raw materials in the formula into 1000 ml of water, stirring for 30min to melt the raw materials, then subpackaging the melted raw materials into 18 x 180 test tubes, 10 ml of each test tube, sealing rubber plug kraft paper, sterilizing in a medical sterilizer at 0.1-0.12 MPa for 25min, and finally sterilizing the expanded strain culture medium.
Opening a freezing tube stored at the temperature of minus 80 ℃ in a super-clean workbench under the aseptic condition, melting at room temperature, then taking 1 tube of the sterilized test tube culture medium, adding 1ml of the melted-80 ℃ storage bacterium liquid into a test tube containing the expanded strain culture medium, sealing the tube opening of the test tube by using a rubber plug and kraft paper, shaking up by hand, putting the test tube into a constant temperature incubator, standing and culturing at the temperature of 37 ℃ for 14 hours, and obtaining the expanded strain culture bacterium liquid.
Second, first-order seed culture of lactobacillus paracasei
The formula of the first-level seed culture medium is as follows: 22 g of glucose, 8 g of peptone, 5 g of beef extract powder, 2g of corn steep liquor, 2g of sodium acetate, 801 ml of tween and 1000 ml of tap water, and the pH value is natural.
Adding the above raw materials into tap water, starting a stirrer, and stirring for 20min to completely melt, which is a first-class seed culture solution. Adding the stirred and melted primary seed culture solution into a triangular flask according to the using amount, then binding the opening of the triangular flask by using 6 layers of gauze and a layer of kraft paper, putting the triangular flask into a medical sterilizer for sterilization, firstly opening an exhaust valve on the sterilizer before sterilization, closing the exhaust valve when a small amount of steam is exhausted, opening the exhaust valve on the sterilizer for exhausting for 6-8min when the pressure on the sterilizer reaches 0.05 MPa, then closing the exhaust valve, continuing heating, starting timing when the steam pressure in the sterilizer reaches 0.1-0.12 MPa, keeping the sterilization time for 25min, leaving a heat source after the sterilization is finished, naturally cooling the sterilizer, opening the sterilizer when the pressure on the sterilizer returns to zero, taking out the sterilized primary seed culture medium, putting the sterilized primary seed culture medium into a superclean bench for naturally cooling, transferring the cultured expanded strain culture solution into the primary seed culture solution of the sterilized triangular flask when the temperature is reduced to 37 ℃, the inoculation amount is 1 percent, namely 100 milliliters of first-stage seed culture solution, 1 milliliter of expanded strain culture solution is inoculated, after the inoculation is finished, the bottle mouth is sealed by using original gauze and kraft paper, the bottle mouth is slightly shaken by hands to uniformly mix the inoculated strain and the culture solution, and then the mixture is cultured in a thermostat for 14 hours at the temperature of 37 ℃, so that the first-stage seed culture solution is obtained.
Third, culturing lactobacillus paracasei seed tank
Sterilizing fermentation equipment, pipelines and a sterile filtration system, firstly opening valves of each inlet and outlet pipeline and a sterile air pipeline, introducing 0.12-0.14 MPa steam, communicating the steam with the pipeline valves and discharging a small amount of steam, introducing the steam for 40min, keeping the steam pressure in the pipelines at 0.12-0.14 MPa for 40min, and then closing valves of each inlet and outlet pipeline for later use.
Sterilizing empty seed tank and fermentation tank, closing valves, opening drain valve at bottom of the tank and drain valve in interlayer, opening direct steam valve, introducing 0.12-0.14 MPa steam into the tank, timing when the temperature in the tank reaches 121 deg.C, sterilizing for 40min, closing the drain valve and interlayer drain valve at bottom of the tank, and naturally cooling to 37 deg.C.
The formula of the seeding tank culture medium (the weight percentage of each component of the culture medium) is as follows: 8% of glucose, 3% of peptone, 0.5% of urea, 0.5% of dipotassium phosphate, 1.5% of corn steep liquor, 0.5% of sodium acetate, 800.4% of tween, 3% of polydextrose, 0.1% of polyether defoamer, 82.5% of tap water and natural pH value.
Firstly, putting tap water into a seeding tank sterilized in an empty tank, starting a stirrer, respectively adding raw materials required in a formula, then introducing steam for heating in continuous stirring, firstly, heating to 95 ℃ by using tank interlayer steam, then, directly heating by using the steam, and keeping for 30min when the temperature in the seeding tank reaches 121 ℃ to achieve the sterilization effect; the steam was then turned off and the tap water cooling through the tank insert was started and was ready for use when the tank temperature reached 37 c, which was called the seeding tank medium. Then, inoculating the cultured primary seed culture bacterial liquid into a seed tank under an aseptic condition, wherein the inoculation amount is 1% of the culture medium in the tank respectively, namely 100kg of the culture medium in the seed tank is inoculated into 1kg of the primary seed culture bacterial liquid respectively. Then, covering an inoculation cap on the tank, and starting stirring culture under the culture conditions: the temperature is 37 ℃, the pH value is natural, the stirring speed is 80 r/min, the tank pressure is 0.05 MPa, the culture time is 22 hours, the requirement can be met, if the tank pressure is reduced, sterile air can be introduced to maintain the tank pressure, and if the tank pressure is too high, the tank pressure can be adjusted by using a gas release valve on the tank top, and the seed tank culture strains are obtained.
Fourth, fermentation culture of lactobacillus paracasei
The fermentation medium comprises the following components in percentage by weight: 12% of glucose, 1% of peptone, 2% of polydextrose, 8% of isomaltooligosaccharide, 1% of sodium acetate, 800.1% of tween, 3% of potato starch, 0.4% of conjugated linoleic acid, 0.3% of anhydrous calcium chloride, 0.5% of magnesium sulfate, 2% of corn steep liquor, 0.1% of polyether defoamer and 69.6% of tap water, and the total weight is 100 kg.
Firstly, the tap water used in the formula is put into a fermentation tank which is sterilized in an empty tank, a stirrer is started, the raw materials used in the formula are put into the fermentation tank, and then steam is introduced for heating. Firstly, heating to 95 ℃ by using interlayer steam, then heating by using direct steam, starting timing when the temperature in the tank reaches 121 ℃, keeping for 30min to achieve the sterilization effect, then cooling by using interlayer tap water, and starting inoculating strains when the temperature in the tank is reduced to 37 ℃. Before inoculation, firstly, the tank pressure of the seeding tank is increased to 0.1 MPa, the tank pressure of the fermentation tank is kept at 0.05 MPa, then, an inoculation pipeline valve is opened, seeds cultured in the seeding tank are conveyed into the fermentation tank in a pressure difference mode, and then, the inoculation pipeline valve is closed.
The inoculation amount is 3kg of strains inoculated into a fermentation tank for culture per 100kg of fermentation medium, the culture condition is that the fermentation temperature is 37 ℃, the stirring speed is 120 r/min, the fermentation time is 18 hours, the pressure of the fermentation tank is kept at 0.05 MPa, the pH value is natural, if the pressure of the fermentation tank is reduced, sterile air can be introduced to keep the pressure of the fermentation tank, and if the pressure of the fermentation tank is too high, the pressure can be adjusted by an exhaust valve at the top of the fermentation tank, so that the zymogen liquid is obtained.
Freeze drying of lactobacillus paracasei bacterial mud
And centrifuging the zymophyte liquid, collecting wet bacterial sludge, pumping the zymophyte liquid into a storage tank after fermentation is finished, and selecting a centrifugal rotating speed is very important in order to ensure the integrity of thalli in the zymophyte. Starting a tubular centrifuge, adjusting the rotating speed to 12000 r/min, centrifuging at the feeding speed of 15kg per minute, collecting wet bacterial sludge after centrifugation is finished, freeze-drying the centrifugally collected wet bacterial sludge, carrying out low-temperature vacuum concentration on the centrifuged fermentation liquor, adding a carrier into the centrifuged fermentation liquor, and compounding the fermented liquor into a finished product after vacuum drying.
1. Preparing suspension liquid, taking 1000 g of wet bacterial mud, adding 3000 ml of sterile distilled water, 300 g of skim milk powder, 200 g of fructo-oligosaccharide, 100ml of 98% glycerol, 50 g of maltodextrin and 300 g of isomaltooligosaccharide. During the specific operation, 1500 ml of water in the formula is firstly heated to 50-55 ℃, maltodextrin is added under stirring and dissolved, then the rest water in the formula is added, and then other raw materials in the formula are added. Stirring thoroughly for 30min (stirring at 25-35 ℃). Obtaining a suspension liquid, and then putting the suspension liquid into a thermostatic chamber or a box with the temperature of 30 ℃ for thermostatic 30min for pre-freezing.
Pre-freezing before freeze drying, namely sub-packaging the suspension liquid with constant temperature for 30min into a freeze drying tray, then pre-freezing the suspension liquid in a pre-freezing chamber of a freeze dryer for 3 hours at the temperature of minus 30-35 ℃, and vacuumizing and drying after freezing. Putting the pre-frozen material tray into a vacuum drying chamber to begin vacuum drying, wherein the drying conditions are as follows: the vacuum degree is 2-5 Pa, the temperature is-45 ℃, the temperature of the drying chamber is 28 ℃, and the drying time is 45 hours. And after freeze-drying, collecting freeze-dried materials, crushing the freeze-dried materials into bacterial powder with the fineness of 100 meshes by using an aseptic crusher, and sealing and packaging the bacterial powder for later use to obtain lactobacillus paracasei bacterial powder.
2. Low-temperature concentration of centrifugal zymophyte liquid: concentrating under vacuum degree of 0.06 MPa at 55-65 deg.C to obtain paste with solid content of 80% and water content of 20%, adding medicinal corn starch as carrier, mixing, and vacuum drying. Taking 1000 g of concentrated paste, adding 200 g of medicinal corn starch, uniformly mixing, and drying in a vacuum drying oven, wherein the drying conditions are as follows: drying at 55-60 deg.C under 0.06 MPa for 45 hr until the water content is 3%, pulverizing to 100 mesh powder, and sealing and packaging to obtain Lactobacillus paracasei metabolite powder.
The probiotic composition with the weight-losing function is compounded as follows: taking 5 g of 100-mesh lactobacillus paracasei powder, 3 g of lactobacillus paracasei metabolite powder, 10 g of microcrystalline cellulose, 20 g of medicinal corn starch, 5 g of inulin and 5 g of magnesium stearate, wherein the microcrystalline cellulose, the medicinal corn starch, the inulin and the magnesium stearate are used as bacteria powder carriers.
Mixing the above materials in solid stirring mixer, packaging under aseptic condition into capsule, and bottling or tabletting to obtain the final product.
Example 2
The probiotic composition with the weight-losing function is compounded as follows: 9 g of 100-mesh lactobacillus paracasei powder, 6 g of lactobacillus paracasei metabolite powder, 22 g of microcrystalline cellulose, 120 g of medicinal corn starch, 12 g of inulin and 12 g of magnesium stearate are taken.
Wherein the lactobacillus paracasei powder and the lactobacillus paracasei metabolite powder are both the lactobacillus paracasei powder and the lactobacillus paracasei metabolite powder prepared in the method of example 1.
Example 3
The probiotic composition with the weight-losing function is compounded as follows: 9 g of 100-mesh lactobacillus paracasei powder, 4 g of lactobacillus paracasei metabolite powder, 22 g of microcrystalline cellulose, 120 g of medicinal corn starch, 8 g of inulin and 8 g of magnesium stearate are taken.
Wherein the lactobacillus paracasei powder and the lactobacillus paracasei metabolite powder are both the lactobacillus paracasei powder and the lactobacillus paracasei metabolite powder prepared in the method of example 1.
Example 4
The probiotic composition with the weight-losing function is compounded as follows: taking 11 g of 100-mesh lactobacillus paracasei powder, 6 g of lactobacillus paracasei metabolite powder, 18 g of microcrystalline cellulose, 80 g of medicinal corn starch, 8 g of inulin and 8 g of magnesium stearate.
Wherein the lactobacillus paracasei powder and the lactobacillus paracasei metabolite powder are both the lactobacillus paracasei powder and the lactobacillus paracasei metabolite powder prepared in the method of example 1.
Example 5
The probiotic composition with the weight-losing function is compounded as follows: taking 11 g of 100-mesh lactobacillus paracasei powder, 6 g of lactobacillus paracasei metabolite powder, 18 g of microcrystalline cellulose, 80 g of medicinal corn starch, 12 g of inulin and 12 g of magnesium stearate.
Wherein the lactobacillus paracasei powder and the lactobacillus paracasei metabolite powder are both the lactobacillus paracasei powder and the lactobacillus paracasei metabolite powder prepared in the method of example 1.
Example 6 significant weight loss of Lactobacillus paracasei of the present examples
1. Design of experiments
72 female C57B6/N mice of 16-20 g body weight were all fed 10% fat energy ratio non-obese control diet (D12450B 10% kcal% fat) under the barrier system for 5 days. After 5 days, the mice were randomly assigned to body weight, and 20 mice continued to be given maintenance diet as a blank control group and a non-obese lactobacillus paracasei group. 52 mice were fed with an obesity-inducing diet (D12492, 60% kcal% fat) at a fat energy ratio of 60% for two weeks as an obesity model group. The food intake, food amount scattered, and food remaining amount were recorded every week, and the body weight was weighed 1 time. The mice given the obesity-induced feed were ranked according to weight gain, and 18 obesity-resistant mice with lower weight gain were eliminated. The 34 screened obesity-sensitive mice were randomly divided into 2 groups according to body weight, namely an obesity model group and an obesity lactobacillus paracasei group, and were continuously fed with the obesity-inducing feed for 9 weeks. The obesity-inducing diet was continued for the obese model group and the obese lactobacillus paracasei group, and the non-obese blank control group of 10 and the non-obese lactobacillus paracasei group of 10 were continued for the control diet for 9 weeks.
The group of 10 non-obese lactobacillus paracasei and the group of 17 obese lactobacillus paracasei fed with the obesity-inducing feed were administered 0.2g of lactobacillus paracasei powder (50 billion/CFU of live lactobacillus paracasei per gram) per day with drinking water, and the group of 17 obese models and the group of 10 non-obese controls were fed as usual. Feeding for 9 weeks, recording food intake, food scattering amount, and food remaining amount every week, and weighing for 1 time. The effect of lactobacillus paracasei on the obesity model was evaluated.
2. Results of the experiment
The lactobacillus paracasei powder can obviously inhibit the weight gain of obese mice, and the lactobacillus paracasei powder is fed for 9 weeks after the obesity induction feed for the obese mice is fed for 2 weeks by C576B/N mice. Table 1 shows that the obese lactobacillus paracasei group significantly reduced the body weight of the mice with the average body weight change during the experiment at week 3, week 7 and week 9 of the feeding. As shown in table 1.
TABLE 1
As can be seen from the data in table 1, the weight of the obese lactobacillus paracasei group was significantly reduced compared to the obese model group, and the weight of the mice could be significantly reduced; the non-obese lactobacillus paracasei group also had a certain reduction compared to the blank control group.
Clinical trial
The effect of the probiotic composition with slimming function in examples 1 to 5 on slimming and fat reduction is shown in table 2.
TABLE 2
As shown in Table 2, 300 persons in the middle-aged male group started to take the composition for 15 days, wherein the fat percentage of 102 persons with different ages was decreased and the food intake was decreased after taking the composition for 15 days; after the medicine is taken for 30 days, the weight of 186 human bodies is reduced by 2-3kg, and the body fat rate is reduced by 0.8%; after the medicine is taken for 60 days, the weight of 248 human bodies is reduced by 5-6kg, and the body fat rate is reduced by 2%; after 90 days of administration, 286 of the human body weight is reduced by 7-8 kg. The body fat rate is reduced by 3 percent; in the test period, the body weight and body fat rate of 14 people who took lactobacillus paracasei did not change.
The statistical effect starts after 300 middle-aged women take the composition for 15 days, wherein the fat rate of 122 people with different ages is reduced and the food intake is reduced after the composition is taken for 15 days; after the medicine is taken for 30 days, 198 human body weight is reduced by 2-3kg, and the body fat rate is reduced by 1%; after 60 days of administration, 262 people have weight reduced by 5-6kg, and the body fat rate is reduced by 2%; after taking for 90 days, the weight of the human body is reduced by 7-8kg for 289 days. The body fat rate is reduced by 3 percent; during the test period, the body weight and body fat rate of 11 persons who took lactobacillus paracasei did not change.
The statistical effect starts when 180 people in the teenager male group take the medicine for 15 days, wherein the fat rate of 68 people with different ages is reduced and the food intake is reduced after the medicine is taken for 15 days; after the medicine is taken for 30 days, the weight of 108 human bodies is reduced by 2-3kg, and the body fat rate is reduced by 0.8%; after the medicine is taken for 60 days, the weight of 149 human bodies is reduced by 5-6kg, and the body fat rate is reduced by 2%; after the medicine is taken for 90 days, the weight of the human body is reduced by 7-8kg by 172 days. The body fat rate is reduced by 3 percent; in the test period, the body weight and body fat rate of 8 persons who took lactobacillus paracasei did not change.
180 teenager female groups begin to take the statistical effect for 15 days, and after taking the medicine for 15 days, 59 human bodies with different ages have reduced fat rate and reduced food intake; after the medicine is taken for 30 days, the weight of 94 human bodies is reduced by 2-3kg, and the body fat rate is reduced by 0.8%; after the medicine is taken for 60 days, 153 of the weight of the human body is reduced by 5-6kg, and the body fat rate is reduced by 2%; after the medicine is taken for 90 days, 170 people have 7-8kg of weight reduction. The body fat rate is reduced by 3 percent; during the test period, the weight and body fat rate of 10 persons who took lactobacillus paracasei did not change.
Although the invention has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Sequence listing
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Claims (6)
1. A probiotic composition with a weight-losing function is characterized in that lactobacillus paracasei bacterium powder and lactobacillus paracasei metabolite powder are used as active ingredients; the lactobacillus paracasei is preserved in China center for type culture Collection with the preservation numbers as follows: CCTCC NO: m2018302; the composition also comprises a fungus powder carrier, wherein the fungus powder carrier comprises microcrystalline cellulose, medicinal corn starch, inulin and magnesium stearate; the composition comprises the following raw materials in parts by mass: 9-11 parts of lactobacillus paracasei powder, 4-6 parts of lactobacillus paracasei metabolite powder, 18-22 parts of microcrystalline cellulose, 80-120 parts of medicinal corn starch, 8-12 parts of inulin and 8-12 parts of magnesium stearate.
2. The probiotic composition with the weight-losing function according to claim 1, wherein the composition comprises the following raw materials in parts by weight: 9.5-10.5 parts of lactobacillus paracasei powder, 4.5-5.5 parts of lactobacillus paracasei metabolite powder, 19-21 parts of microcrystalline cellulose, 90-110 parts of medicinal corn starch, 9-11 parts of inulin and 9-11 parts of magnesium stearate.
3. A method for preparing the composition of claim 1 or 2, wherein lactobacillus paracasei is subjected to expanded strain culture, primary seed culture, seed tank culture and fermentation culture to obtain lactobacillus paracasei zymocyte fluid; centrifuging the lactobacillus paracasei fermented liquid, respectively collecting lactobacillus paracasei bacterial sludge and centrifuged fermented liquid, and respectively freezing, drying, crushing, concentrating, drying and crushing the lactobacillus paracasei bacterial sludge and the centrifuged fermented liquid to obtain lactobacillus paracasei bacterial powder and lactobacillus paracasei metabolite powder; and mixing the lactobacillus paracasei bacterial powder, the lactobacillus paracasei metabolite powder and a bacterial powder carrier to obtain the composition.
4. The method of claim 3, wherein the preparation method of the Lactobacillus paracasei powder comprises the following specific steps: and mixing the lactobacillus paracasei bacterial sludge, sterile water, skim milk powder, fructo-oligosaccharide, glycerol, maltodextrin and isomalto-oligosaccharide to obtain lactobacillus paracasei suspended bacterial liquid, and freeze-drying and crushing the suspended bacterial liquid to obtain lactobacillus paracasei bacterial powder.
5. The method of claim 4, wherein the suspension is freeze-dried at-35 ℃ to-30 ℃ for 2-5 hours, vacuum freeze-dried at-50 ℃ to-40 ℃ and crushed into 100 mesh powder of Lactobacillus paracasei.
6. Use of a composition prepared by the method of claim 3 in the preparation of a product having at least one of the following functions: 1) losing weight; 2) promoting metabolism; 3) reducing blood fat; 4) enhancing immunity.
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| PT2123168E (en) * | 2008-05-16 | 2012-03-28 | Nestec Sa | Lactobacillus paracasei and weight control |
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