CN109923967A - The diffusive transport method of multiple resistance pollutant in earthworm excrement is controlled in a kind of biomass carbon resistance - Google Patents
The diffusive transport method of multiple resistance pollutant in earthworm excrement is controlled in a kind of biomass carbon resistance Download PDFInfo
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Abstract
The diffusive transport method of multiple resistance pollutant in earthworm excrement is controlled in a kind of biomass carbon resistance, is related to agricultural environment field of engineering technology.Biomass carbon and earthworm excrement are mixed, mixing mass ratio is 1:200 ~ 1:50, and stands at least 7 days, keeps 60% field capacity.Resistance pollutant refers to antibiotic, resistance pathogenic bacteria and resistant gene.This method has been filled up lacks in a kind of effectively resistance control earthworm excrement resistance pollutant in the blank of earthworm excrement-soil-crop process diffusive transport technology in the prior art；The present invention provides suitable Multiple Classes of Antibiotics, resistance pathogenic bacteria and resistant gene contaminated soils, caused compound new pollution in agricultural land soil is administered to for reduction earthworm excrement and provides high-efficiency broad spectrum and environmentally protective method for blocking and controlling, be can be applied to agricultural and is especially applied the Product quality and safeties field such as livestock and poultry organic fertilizer (earthworm excrement) and crops.
Description
Technical field
The present invention relates to agricultural environment engineering combined contamination soil recovery technique fields, especially biomass carbon resistance control earthworm excrement
The diffusive transport method of middle multiple resistance pollutant.
Background technique
Wormcast is a kind of natural ecology and environmentally friendly resource, has short texture, large specific surface area, containing higher
The nutrient elements such as nitrogen phosphorus, composting process does not generate the advantages such as waste, thus earthworm excrement is commonly used for the neck such as crop-planting, vegetable growth
Domain.In recent years, due to preparing the initial substrate multi-source of earthworm excrement in the feces of livestock and poultry such as cow dung, pig manure, chicken manure, these initial organic fertilizers
Expect that antibiotic residue status is more universal in matrix.In earthworm excrement remaining antibiotic and thus caused by antibiotics resistance gene
The problem of (Antibiotic resistance gens, ARGs) overexpression, is highly paid close attention to.Due to lacking for environmental management
It loses, the abuse of antibiotic usually can induce and filter out a large amount of resistance endogenetic bacterias in animal and bird intestines, since livestock and poultry can not incite somebody to action
Antibiotic is metabolized completely, can be excreted in excrement in the form of parent compound or mesostate, thus is also resulted in
Antibiotic-resistant bacteria (Antibiotic resistant bacteria, ARB) high abundance residual and preservation in earthworm excrement.
Biomass carbon (Biochar) is biological material under conditions of complete or partial anoxic, is formed through pyrolysis charring
A kind of phosphorus content substance abundant, have the characteristics that large specific surface area, porous, oxygen-containing functional group rich in, it is right
Pollutant in soil has certain adsorption function, reduces environmental pollution, and improves soil quality.Due to preparing biomass carbon
Biological material is different, and the function of the physicochemical properties of biomass carbon and absorption pollutant is therefore related there are larger difference
Research of the biomass carbon to pollutant in soil environment behavior receives more and more attention.Biomass carbon is mainly raw with plant source
Based on substance charcoal, including the wood chips charcoal such as the straw charcoals such as corn stover, wheat stalk and pine tree, cypress, and straw charcoal compares wood chip
The gap of charcoal is bigger, and adsorption capacity is more excellent.Biomass carbon can for solve the organic fertilizer applications such as earthworm excrement into soil antibiotic,
The resistance control of resistance pathogenic bacteria and resistant gene diffusion provides a kind of feasible technical method.
Major defect of the existing technology is: being mainly at present biomass carbon about the patent research of biomass carbon
Preparation or method of modifying, the improvement and application of biomass carbon fertilizer, reparation and modified biomass of the biomass carbon to contaminated soil
Removal absorption etc. of the charcoal to heavy metal, but the antibiosis in the organic fertilizer such as control feces of livestock and poultry is hindered to biomass carbon broad spectrum activity
The research of element, resistance pathogenic bacteria and resistant gene this kind emerging pollutant is less.Research emphasis is that earthworm excrement is applied at present
Can promote crop growth in agricultural land soil, but lack to earthworm excrement be administered in soil and crops such as planting vegetable etc. during
Caused by the resistance control measure of antibiotic, resistance pathogenic bacteria and resistant gene diffusive transport probe into, resist in process of crop growth
This ecological risk of diffusive transport of element and its resistant gene pollution is given birth to almost without being taken seriously.
By the exploration of pertinent literature and patent, finds no pass biomass carbon and earthworm excrement is administered in agricultural land soil and is planted
The resistance control technology for planting resistance pollutant diffusive transport during crops being published and accepting, and of the invention immediate existing
Method is biomass carbon or other methods to pollutants such as heavy metal, antibiotic, resistant genes in waste water, contaminated soil
The related application of minimizing technology.Chinese invention application number: CN201710041203.9, CN201810634312.6,
CN201610907752.5, CN201710434497.1, CN201711149005.0, CN201710983680.7 are biology respectively
Technology of the matter charcoal to heavy metal copper contaminated soil remediation, the side that modified biomass charcoal and its resistance control heavy metal add up in vegetables body
Method, the method for antibiotic in graphene catalysis ozone/modification sugarcane active carbon degrading waste water, electrode method remove marine culture wastewater
In/Azolla imbricata phytoremediation technology repairing and treating soil in resistant gene processing method,.Current existing technology is mainly given birth to
Substance charcoal resistance control soil in resistance pollutant be enriched with to plant vivo migration method, plant cover cultivation methods with apply (application number:
201810639603.4), however for the resistance control technology of the diffusive transport of multiple resistance pollutant in biomass carbon resistance control earthworm excrement
Almost without.Application number: CN201710041203.9 provides a kind of modified biomass charcoal and its resistance control heavy metal in vegetables body
Accumulative method prepares the mineral modified biomass carbon that pH value is 7-8 by dissolution-concussion-ultrasound-pyrolytic process, is applied to
The enrichment of vegetables edible portion heavy metal is reduced in rhizosphere soil；Application number: CN201810634312.6 provides a kind of biology
Matter charcoal inhibits the method that copper is migrated into crops in heavy metal cuprum polluted soil, is prepared with common biological material specific
For heavy metal cuprum polluted soil biomass carbon, pollutant in soil copper adsorb and fix avoiding turning to crops
It moves；Application number: CN201610907752.5 provides a kind of method of antibiotic in graphene catalysis ozone degrading waste water, utilizes nitrogen
Non-metallic catalyst is made in two kinds of atom doped modified graphenes of old metal of phosphorus, improves ozone oxidation efficiency, farthest drops
The residual quantity of low antibiotic in water；Application number: CN201710434497.1 provides a kind of modified sugarcane active carbon and goes in water removal
Firing processing at sugarcane skin is prepared sugarcane active carbon, and carries out modification and go resisting in water removal by the method for antibiotic
Raw element；Application number: CN201711149005.0 provides a kind of processing method for removing resistant gene in marine culture wastewater, passes through
Resistant gene in the removal waste water such as physics removal of impurities pond, electrolytic cell, application number: CN201710983680.7 provides a kind of Azolla imbricata
The method that resistant gene pollutes in phytoremediation chronic administration livestock and poultry muck and the soil of sewage irrigation can effectively hinder in control soil
The relative abundance of antibiotic, content of beary metal and total resistant gene, transposase gene.
These types of method all indicates heavy metal, antibiotic, resistant gene etc. in the removal environment such as waste water or contaminated soil
The method of pollutant, but generally lack the method for combined pollutant diffusive transport in resistance control earthworm excrement, moreover, existing patent is even more
It is not related to using biomass carbon impedance earthworm excrement-Soil-crops process multiple resistance pollutant diffusive transport method.
The main reason for defect generates has: in recent years, more and more research consciousness to feces of livestock and poultry-Soil-crops
System will cause the diffusive transport risk of antibiotic in environment, resistance pathogenic bacteria and resistant gene, and can pass through different micro- lifes
Plasmid horizontal transfer between object threatens human health through food chain.Existing research in the feces of livestock and poultry such as pig manure, cow dung mainly to deposit
Based on combined pollutant, but to the research of combined pollution in the earthworm excrement that feces of livestock and poultry is initial substrate almost without thus needle
To probing into less there are the resistance control technology of antibiotic and resistant gene combined pollution in earthworm excrement, patent relevant to earthworm excrement is not
Show earthworm excrement to ecological bring environmental risk.Therefore, carry out for resistance control earthworm excrement-Soil-crops system in antibiotic and
The technology of the horizontal risk of resistant gene it is extremely important with it is urgent.
Summary of the invention
The technical issues of solution: the present invention is directed to above-mentioned prior art defect, provides in a kind of biomass carbon resistance control earthworm excrement
The diffusive transport method of multiple resistance pollutant, the application is towards application earthworm excrement etc. feces of livestock and poultry and plants the farmland soils of crops
Earth, resistance control planting process in antibiotic, tolerant bacteria and resistant gene combined pollution diffusive transport and remove resistant gene
Repair mode, it is a kind of valence high-efficiency broad spectrum that repairing, which terminates soil ecology risk, which reduces, and lattice are cheap and the agricultural environment of green close friend
Recovery technique.
Technical solution: the diffusive transport method of multiple resistance pollutant in earthworm excrement is controlled in a kind of biomass carbon resistance, including as follows
Step: biomass carbon and earthworm excrement are mixed, and mixing mass ratio is 1:(5~2), and at least 7 days are stood, keep 60wt.%
Field capacity.
Above-mentioned resistance pollutant is antibiotic, resistance pathogenic bacteria and resistant gene.
Above-mentioned biomass carbon is plant source biomass carbon, and partial size is 80~100 mesh, and specific surface area is 100~300m2/g。
Above-mentioned plant source biomass carbon is straw charcoal or wood chip carbon made of firing through 450 DEG C, and the straw charcoal is corn
Stalk or wheat stalk；The wood chip carbon is pine tree or cypress.
Above-mentioned wormcast is the organic fertilizer that initial substrate is cow dung, pig manure or chicken manure.
Above-mentioned biomass carbon mixed with earthworm excrement after to be applied with soil by the mass ratio of 1:200~1:40, then carry out farming
The plantation of object, the crops include at least one of leaf vegetables crops, root-tuber crop and fruit class crops；Institute
Stating leaf vegetables crops includes at least one of romaine lettuce, green vegetables, Chinese cabbage, spinach, leaf mustard；The root-tuber crop includes Ma Ling
At least one of potato, carrot, Chinese yam；The fruit class crops include cucumber, capsicum, tomato, in corn at least
It is a kind of.
Above-mentioned antibiotic includes that Tetracyclines, sulfamido, macrolides, quinolones, chloromycetin are often anti-with veterinary drug
At least one of raw element；Resistance pathogenic bacteria include gram-positive bacteria and Gram-negative bacteria, wherein Gram-positive resistance bacterium
Including bacillus, enterococcus spp；Gram-negative antibiotic-resistant bacteria includes acinetobacter, Proteus, pseudomonad
At least one of category, Colibacter, Arthrobacter；Resistant gene include tetM, tetO, tetW, sul1, sul2, mecA,
At least one of qnrA, qnrD, cmlA, floR.
The working principle of the invention is: 1, biomass carbon is biological material under conditions of complete or partial anoxic, warp
A kind of phosphorus content substance abundant that pyrolysis charring is formed, the black solid substance that mostly particle is careful, quality is lighter, tool
There is the features such as large specific surface area, porous, oxygen-containing functional group rich in；2, biomass carbon has one to the pollutant in soil
Fixed adsorption function, reduces environmental pollution, and improves soil quality, and compared with wood chip charcoal, straw charcoal specific surface area is bigger, absorption effect
Fruit is much better；3, biomass carbon contains the oxygen-containing functional groups such as carboxyl, hydroxyl, mainly passes through exclusive absorption and non-exclusive absorption
To adsorb the antibiotic in soil, hydroxyl and antibiotic the formation inner-sphere complex of soil surface, to chemically adsorb
Antibiotic；And cation is bonded and the atomic interaction with soil surface, is used between adion and soil surface
Weaker covalent key connection forms outer-sphere complex.4. since biomass carbon has a large amount of gap, in adsorption process, in solution
Ion can be diffused in gap, to be attracted to inside biomass carbon.Since adsorbed material is in different soda acid items
Hydrolysis degree is different under part, and the pH of medium can have a great impact to adsorption effect.
The utility model has the advantages that the present invention is for antibiotic and resistant gene in biomass carbon resistance control earthworm excrement-Soil-crops system
The method of combined pollution provides a kind of quick recovery technique.Its major advantage having is: 1. biomass carbon sources are wide
It is general, about 700,000,000 tons of the stalks such as rice, corn annual output, and it is cheap, it is a kind of good material；2. biomass carbon have than
It the features such as surface area big, porous, rich in oxygen-containing functional group, can efficiently and broad spectrum activity absorption Multiple Classes of Antibiotics, resistance cause
Germ and resistant gene pollutant；3. biomass carbon and earthworm excrement can improve agricultural land soil structure simultaneously, edaphon knot is maintained
The stability of structure and quantity；4. biomass carbon and the preparation of earthworm excrement are simple, easily stored and transport, easily operated, biomass carbon is
Green, environmentally friendly a kind of material.This method is controlled antibiotic for the raising and resistance of China's agricultural land soil crop yield, is resisted
The diffusive transport risk of property bacterium and resistant gene combined pollution is with a wide range of applications.
Detailed description of the invention
Fig. 1 is biomass carbon (left figure is straw charcoal, and right figure is wood chip charcoal) scanning electron microscopic picture；
Fig. 2 is that earthworm excrement is applied to tetracycline and sulfanilamide (SN) in soil before and after the embodiment of the present invention 1 applies straw charcoal and wood chip charcoal
Pyrimidine contains spirogram；
Fig. 3 is that earthworm excrement is applied to tetracycline and sulfanilamide (SN) in soil before and after the embodiment of the present invention 1 applies straw charcoal and wood chip charcoal
Pyrimidine resistant gene abundance figure；
Fig. 4 is that earthworm excrement is applied to the romaine lettuce blade that soil is planted before and after the embodiment of the present invention 2 applies straw charcoal and wood chip charcoal
Green fluorescence protein gene (gfpmut3a) abundance figure；
Fig. 5 is that earthworm excrement is applied to the romaine lettuce blade that soil is planted before and after the embodiment of the present invention 2 applies straw charcoal and wood chip charcoal
Middle macrolide and quinolone resistant gene abundance figure；
Fig. 6 is that earthworm excrement is applied to the potato ball that soil is planted before and after the embodiment of the present invention 3 applies straw charcoal and wood chip charcoal
Resistance pathogenic bacteria number figure in stem；
Fig. 7 is that earthworm excrement is applied to the potato ball that soil is planted before and after the embodiment of the present invention 3 applies straw charcoal and wood chip charcoal
Chloramphenicol and sulfamerazine resistant gene abundance figure in stem；
Fig. 8 is that earthworm excrement is applied to tetracycline and sulfanilamide (SN) in soil before and after comparative example 4 of the present invention applies straw charcoal and wood chip charcoal
Pyrimidine resistant gene abundance figure.
Specific embodiment
The following specific embodiments technical solution that the invention is not limited in any way, it is all to use equivalent replacement or wait
The mode technical solution obtained of effect transformation all falls within protection scope of the present invention.
The diffusive transport method of multiple resistance pollutant in earthworm excrement is controlled in a kind of biomass carbon resistance, is included the following steps:
Biomass carbon and earthworm excrement are mixed, at least 7 days or more is stood, keeps the field capacity of 60wt.%.It is anti-
Contact scar object refers to antibiotic, tolerant bacteria and resistant gene.
Above-mentioned biomass carbon refers to one kind of the wood chips charcoal such as the straw charcoals such as corn stover, wheat stalk and pine tree, cypress
Or a variety of is raw material, is fired through 450 DEG C of high temperature, pH has porous, large specific surface area in alkalinity, biomass carbon, contains hydroxyl
The functions such as base, carboxyl group can be adsorbed antibiotic and its be resisted by hydrogen bond, ionic bond etc..
Above-mentioned field capacity refers to the maximum amount of water of soil conservation after bulk water exclusion under field condition, is to measure soil
The important indicator of water-retaining property.
One kind that above-mentioned earthworm excrement refers to that earthworm is obtained fermentation of organic wastes by 7-14 days digestions is organic
The general designation of fertilizer has short texture, and large specific surface area, containing compared with nutrient elements such as high nitrogen phosphorus, composting process does not generate waste etc.
Advantage.The initial substrate multi-source of earthworm excrement is prepared in feces of livestock and poultry such as cow dung, pig manure, chicken manures, and residual in initial organic fertilizer matrix
The status that the antibiotic and heavy metal stayed coexists is more universal.
Above-mentioned antibiotic is also referred to as antibacterial agent, has as caused by microorganism and inhibits other quasi-microorganism growths, life
A kind of secondary metabolite deposited, and chemically synthesis or semi-synthetic similar compound.Make with antibacterial or sterilization
With there are mainly four types of the mechanisms of action: hindering the synthesis of bacteria cell wall；With bacterial cell membrane interaction, enhance bacterial cell
The permeability of film；It is mutually used with bacterial ribosome or its reaction substrate, inhibit the synthesis of protein；Hinder bacterium deoxyribose
The duplication and transcription of nucleic acid.Common mainly has the antibiosis such as Tetracyclines, sulfamido, quinolone, chloramphenicol, macrolides
Element.
Above-mentioned resistant gene refers to the modes such as random mutation or the potential resistant gene of expression, makes to deposit on genome in bacterial body
Resistant gene prototype, quasi- resistant gene or potential resistant gene be expressed, so that the antibiotic for obtaining bacterium is anti-
Property.The residual of antibiotic can induce and filter out antibiotic-resistant bacteria in environment, and scale occurred so as to cause intracorporal resistant gene
It reaches.Antibiotics resistance gene is a kind of novel environmental contaminants, diffusion can occur by horizontal transfer in different media and pass
It broadcasts, ecological risk is brought to environment.
The present invention is further illustrated below by specific embodiment and comparative example, it should be understood, however, that, these implementations
Example, which is suitable only for being described in more detail, to be used, and but should not be understood as present invention is limited in any form.
Embodiment 1
The embodiment of biomass carbon resistance control cow dung source earthworm excrement tetracycline, sulphadiazine and its corresponding resistant gene
Target soil: the agricultural land soil of the cow dung source earthworm excrement application of Yin Fen factory, Zhenjiang, Jiangsu Jurong City.Soil is managed substantially
Change property: pH 7.1, organic matter 23.2gkg-1, full nitrogen 2.9gkg-1, full phosphorus 1.7gkg-1, rapid available phosphorus 75.3gkg-1,
Full potassium 15.6gkg-1, available potassium 152.4gkg-1；Earthworm excrement physicochemical property: pH 6.4, organic matter 305.4gkg-1, full nitrogen
14.5g·kg-1, full phosphorus 10.1gkg-1, rapid available phosphorus 398.0gkg-1, full potassium 10.8gkg-1, available potassium 737.5gkg-1。
The antibiotic mean concentration of soil is respectively after the application of earthworm excrement, and tetracycline is 12.4 ± 0.2mgkg-1, sulfanilamide (SN) is phonetic
Pyridine is 15.9 ± 0.3mgkg-1, the absolute abundance point of Tetracyclines resistant gene tetM, tetO, tetW in every gram of fresh weight soil
It is not 7.2 × 106, 6.4 × 108, 4.6 × 107Copy number, every gram of fresh weight Sulfadiazine in Soil resistant gene sul1, sul2's
Absolute abundance is 9.7 × 10 respectively6, 4.1 × 108Copy number.
Pine branches and wheat stalk are respectively obtained into wood chip charcoal and straw charcoal, biomass carbon after 450 DEG C are fired first
It sieves with 100 mesh sieve after grinding, stirs and evenly mixs biomass carbon and cow dung source earthworm excrement by the mass ratio of 1:5, kept for 10 days naturally, and will
The aggregate sample of the agricultural land soil of earthworm excrement/biomass carbon and 0-30cm is mixed by 1:200 mass ratio, is stood 5 days, is remained
The maxmun field capacity of 60wt.%, with the 10 of every 1kg earthworm excrement/soil inoculation 100mL9CFU/mL band red fluorescence marks
(RFP) it is cultivated 60 days after pseudomonas aeruginosa PaoI.
Embodiment 2
The embodiment of biomass carbon resistance control pig manure source earthworm excrement macrolide, quinolone and its corresponding resistant gene
Target soil: the agricultural land soil of the pig manure source earthworm excrement application of Yin Fen factory, Nantong city.Soil after the application of earthworm excrement
Antibiotic mean concentration be respectively, macrolide be 8.7 ± 0.2mgkg-1, quinolone is 5.3 ± 0.3mgkg-1, often
The absolute abundance of macrolide resistance gene mecA is 3.6 × 10 in gram fresh weight soil7Copy number, quinoline promise in every gram of fresh weight soil
The absolute abundance of ketone resistant gene qnrA, qnrD are 4.2 × 10 respectively6, 7.4 × 106Copy number.
Cypress branch and wheat stalk are respectively obtained into wood chip charcoal and straw charcoal, biomass carbon after 450 DEG C are fired first
60 meshes are crossed after grinding, stir and evenly mix biomass carbon and pig manure earthworm excrement by the mass ratio of 1:2, naturally holding 10 days, and by earthworm
The aggregate sample of the agricultural land soil of excrement/biomass carbon and 0-30cm is mixed by 1:100 mass ratio, is stood 5 days, is remained
The maxmun field capacity of 60wt.%, and with the 10 of every 1kg earthworm excrement/soil inoculation 80mL9CFU/mL band green fluorescence marks
(GFP) and the e. coli k12 containing toxin.
Leaf vegetables crops romaine lettuce is then planted, uses conventional water and fertilizer management in romaine lettuce planting process, to romaine lettuce maturation
Afterwards, it is tested and analyzed with sterile water wash romaine lettuce blade.
Embodiment 3
The embodiment of biomass carbon resistance control chicken manure source earthworm excrement chloramphenicol, sulfamerazine and its corresponding resistant gene
Target soil: the agricultural land soil of the chicken manure source earthworm excrement application of horizontal small stream Yin Fen factory, Nanjing city.After the application of earthworm excrement
The antibiotic mean concentration of soil is that chloramphenicol is 4.2 ± 0.1mgkg respectively-1, sulfamerazine is 10.9 ± 0.3mg
kg-1, the absolute abundance of chloramphenicol resistance gene cmlA, floR are 9.5 × 10 in every gram of fresh weight soil7, 6.1 × 106Copy number,
The absolute abundance of sulfamerazine resistant gene sul1, sul2 are 6.9 × 10 respectively in every gram of fresh weight soil6, 3.6 × 107Copy
Number.
Pine branches and corn stover are respectively obtained into wood chip charcoal and straw charcoal, biomass carbon after 450 DEG C are fired first
80 meshes are crossed after grinding, stir and evenly mix biomass carbon and chicken manure source earthworm muck earth by the mass ratio of 1:5, are kept for 10 days naturally,
And mix the aggregate sample of earthworm excrement/biomass carbon and the agricultural land soil of 0-30cm by 1:100 mass ratio, 5 days are stood, is remained
The maxmun field capacity of 60wt.%, and cultivate 60 days.Detection finds to contain resistant pathogenic bacteria in the earthworm excrement in early-stage study
E. coli k12 and pseudomonas aeruginosa paoI.
Then plantation root-tuber crop potato, potato planting is in the process using conventional water and fertilizer management and protection pipe
Reason is tested and analyzed after potato is mature with sterile water wash potato.
Comparative example 1
Comparative example 1 the difference from embodiment 1 is that soil without any processing (not adding any biomass carbon), directly
Apply cow dung source earthworm excrement according to 1 mode of embodiment.
Comparative example 2
Comparative example 2 the difference from example 2 is that soil without any processing (not adding any biomass carbon), directly
Apply pig manure source earthworm excrement according to 2 mode of embodiment.
Comparative example 3
The difference of comparative example 3 and embodiment 3 be soil without any processing (not adding any biomass carbon), directly
Apply chicken manure source earthworm excrement according to 3 mode of embodiment.
Comparative example 4
After comparative example 4 is only that soil is mixed with biomass carbon with the difference of embodiment 1, do not keep 7 days or more, but it is straight
It connects and is mixed with earthworm excrement.
Test example
Measure the antibiotic content, antibiotics resistance gene analyzed in embodiment 1 and 4 soil of comparative example 1 and comparative example
Type and absolute abundance, embodiment 1 and the testing result of comparative example 1 and comparative example 4 are as shown in table 1.
The testing result of table 1 embodiment 1 and comparative example 1 and comparative example 4
Measurement analysis embodiment 2 and 2 soil of comparative example in antibiotic content, antibiotics resistance gene type and absolutely
Abundance, embodiment 2 and the testing result of comparative example 2 are as shown in table 2.
The testing result of table 2 embodiment 2 and comparative example 2
Measurement analysis embodiment 3 and 3 soil of comparative example in antibiotic content, antibiotics resistance gene type and absolutely
The testing result of abundance, embodiment 3 and comparative example 3 is as illustrated in chart 1.
The testing result of table 3 embodiment 3 and comparative example 3
By the testing result of embodiment 1 and comparative example 1 in table 1 as it can be seen that such as Fig. 2, the cow dung source earthworm through biomass carbon processing
Antibiotic content is decreased obviously than antibiotic content in original cow dung source earthworm muck earth in excrement application soil, and straw charcoal compares wood chip
The effect of charcoal is more preferable；Such as Fig. 3, antibiotics resistance gene type and rich in the cow dung source earthworm excrement application soil that is handled through biomass carbon
Degree is decreased obviously than antibiotics resistance gene abundance in original cow dung source earthworm excrement.The result shows that biomass carbon can efficiently hinder control ox
Excrement source earthworm excrement applies soil tetracycline, sulphadiazine and its resistant gene.
By the testing result of embodiment 2 and comparative example 2 in table 2 as it can be seen that the pig manure source earthworm excrement after biomass carbon addition is applied
Soil antibiotic content is decreased obviously than antibiotic content in original pig manure source earthworm muck earth, and straw charcoal hinders control energy than wood chip charcoal
Power is stronger；Green fluorescence mark is had in the romaine lettuce blade planted such as Fig. 4, the pig manure source earthworm excrement application soil after biomass carbon addition
The gene abundance of note significantly reduces, and straw charcoal becomes apparent from than the reduction of wood chip charcoal；Pig manure source such as Fig. 5, after biomass carbon addition
Antibiotics resistance gene abundance is than antibiotic resistance base in original pig manure source earthworm excrement in the romaine lettuce blade of earthworm excrement application soil plantation
Because abundance is decreased obviously.The result shows that biomass carbon can effectively hinder control pig manure source earthworm excrement application soil macrolide, quinolone and
Its resistant gene reduces ecological risk.
By the testing result of embodiment 3 and comparative example 3 in table 3 as it can be seen that plant source biomass carbon treated chicken manure source earthworm
Antibiotic content is decreased obviously than antibiotic content in original chicken manure source earthworm muck earth in excrement application soil, and straw charcoal is compared with wood chip
Charcoal is more preferable to the absorption abatement effect of antibiotic；Such as Fig. 6, treated the chicken manure source earthworm excrement application soil plantation of material resource biomass carbon
Potato in resistance pathogenic bacteria quantity than reducing an order of magnitude in original chicken manure source earthworm muck earth, and straw charcoal is compared with wood chip
The effect of charcoal antagonism flora resistance control is more preferable；Such as Fig. 7, treated the chicken manure source earthworm excrement application soil plantation of plant source biomass carbon
Potato in antibiotics resistance gene abundance be decreased obviously than antibiotics resistance gene abundance in original chicken manure source earthworm excrement.Knot
Fruit shows straw charcoal and wood chip charcoal both plant source biomass carbons to resistance control chicken manure source earthworm excrement application soil chloramphenicol, sulphur
Amine first pyrimidine and its resistant gene have preferable effect.
It can be obtained by the analysis result of embodiment 1 in table 1 and comparative example 4, need to stand after biomass carbon and pedotheque mixing
For a period of time, be conducive to accelerate the degradation of antibiotic and resistant gene.As shown in table 1 and Fig. 8, biomass carbon is added to farmland soil
In earth but activated processing to earthworm excrement application soil in antibiotic concentration and resistant gene abundance resistance control effect than without
The processing of activation is more effective, thus, it is more necessary that biomass carbon stands a period of time after mixing with pedotheque.
Wood chip charcoal and straw charcoal are fired by pine branches and 450 DEG C of wheat stalk respectively, and with barium chloride-sulfuric acid
The cation exchange capacity (CEC) of exchange process measurement biomass carbon and soil is forced, the cation exchange capacity (CEC) of wood chip charcoal is 60.94cmol/
Kg, straw charcoal are 105.37cmol/kg, and soil is 13.76cmol/kg.The cation exchange capacity (CEC) of biomass carbon is obviously excellent
In soil, and straw charcoal is more due to wood chip charcoal, thus the addition of biomass carbon significantly promote the sun in earthworm excrement application soil from
Sub- exchange capacity, to promote the absorption to multiple resistance pollutant in earthworm excrement.
Claims (7)
1. a kind of diffusive transport method of multiple resistance pollutant in biomass carbon resistance control earthworm excrement, it is characterised in that including walking as follows
It is rapid: biomass carbon and earthworm excrement are mixed, mixing mass ratio be 1:(5 ~ 2), and stand at least 7 days, by biomass carbon with
Agricultural land soil is added in the mixture of earthworm excrement, and keeps the field capacity of 60wt.%.
2. the diffusive transport method of multiple resistance pollutant, special in biomass carbon resistance control earthworm excrement according to claim 1
Sign is that the resistance pollutant is antibiotic, resistance pathogenic bacteria and resistant gene.
3. the diffusive transport method of multiple resistance pollutant, special in biomass carbon resistance control earthworm excrement according to claim 1
Sign is that the biomass carbon is plant source biomass carbon, and partial size is 80 ~ 100 mesh, and specific surface area is 100 ~ 300 m2/g。
4. the diffusive transport method of multiple resistance pollutant, special in biomass carbon resistance control earthworm excrement according to claim 3
Sign is that the plant source biomass carbon is straw charcoal or wood chip carbon made of firing through 450 DEG C, and the straw charcoal is corn stalk
Stalk or wheat stalk；The wood chip carbon is pine tree or cypress.
5. the diffusive transport method of multiple resistance pollutant, special in biomass carbon resistance control earthworm excrement according to claim 1
Sign is that the wormcast is the organic fertilizer that initial substrate is cow dung, pig manure or chicken manure.
6. the diffusive transport method of multiple resistance pollutant, special in biomass carbon resistance control earthworm excrement according to claim 1
Sign is with the mass ratio application with soil by 1:200 ~ 1:40 after the biomass carbon is mixed with earthworm excrement, then carries out crops
Plantation, the crops include at least one of leaf vegetables crops, root-tuber crop and fruit class crops；It is described
Leaf vegetables crops include at least one of romaine lettuce, green vegetables, Chinese cabbage, spinach, leaf mustard；The root-tuber crop includes Ma Ling
At least one of potato, carrot, Chinese yam；The fruit class crops include cucumber, capsicum, tomato, in corn at least
It is a kind of.
7. the diffusive transport method of multiple resistance pollutant, special in biomass carbon resistance control earthworm excrement according to claim 2
Sign is that the antibiotic includes that Tetracyclines, sulfamido, macrolides, quinolones, chloromycetin often use veterinary drug antibiosis
At least one of element；Resistance pathogenic bacteria include gram-positive bacteria and Gram-negative bacteria, wherein Gram-positive resistance bacterium bag
Include bacillus, enterococcus spp；Gram-negative antibiotic-resistant bacteria include acinetobacter, Proteus, pseudomonas,
At least one of Colibacter, Arthrobacter；Resistant gene includestetM、tetO、tetW、sul1、sul2、mecA、qnrA、qnrD、cmlA、floAt least one of R.
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