CN109922814B

CN109922814B - Method for preparing nose drops composition

Info

Publication number: CN109922814B
Application number: CN201880004268.9A
Authority: CN
Inventors: 石井阳一; 冈本猛; 牧野纱也香
Original assignee: Ishi Corp
Current assignee: Ishi Corp
Priority date: 2017-03-21
Filing date: 2018-03-16
Publication date: 2020-10-30
Anticipated expiration: 2038-03-16
Also published as: US20190365825A1; KR20190060862A; WO2018173971A1; JP6501135B2; TW201902497A; TWI685342B; CN109922814A; US11642380B2; US20210322487A1; AU2018237807A1; EP3603652A1; US11077152B2; JPWO2018173971A1; EP3603652A4; KR101998647B1; AU2018237807B2; EP3603652B1

Abstract

The present invention provides a method for producing a nasal drop composition suitable for treating or preventing diseases or symptoms in the nasal cavity, and a nasal drop composition obtained by the production method. The method for producing a nasal drop composition and the nasal drop composition obtained by the production method are characterized by comprising the following steps: a mixing step of mixing the earthworms with water; and a recovery step of recovering the vaporized water generated from the mixture obtained in the mixing step to obtain a liquid. In the mixing step, it is preferable that the organic material is further mixed with the earthworms' manure and the water.

Description

Method for preparing nose drops composition

Technical Field

The present invention relates to a method for producing a nasal drop composition suitable for treating or preventing a disease or a symptom in a nasal cavity, and a nasal drop composition obtained by the production method.

Background

Heretofore, nasal drop compositions have been used for the treatment and prevention of pollinosis or pyorrhea. Various studies have been made on such nasal drop compositions (for example, patent documents 1 and 2), but as a general theory, there is a demand for nasal drop compositions different from the conventional ones in terms of difficulty in exhibiting the effect depending on the subject.

Documents of the prior art

Patent document

Patent document 1: japanese patent No. 4591810

Patent document 2: japanese patent No. 4790096

Disclosure of Invention

Problems to be solved by the invention

Accordingly, an object of the present invention is to provide a method for producing a nasal drop composition suitable for treating or preventing a disease or a symptom in a nasal cavity, and a nasal drop composition obtained by the production method.

Means for solving the problems

The present inventors have conducted intensive studies to solve the above problems, and as a result, have found that a liquid obtained by recovering water vaporized when earthworm soil is mixed with water is suitable for the treatment or prevention of diseases or symptoms in the nasal cavity, and have completed the present invention.

That is, the method for producing a nasal drop composition of the present invention is characterized by comprising the steps of: a mixing step of mixing the earthworms with water; and a recovery step of recovering the vaporized water generated from the mixture obtained in the mixing step to obtain a liquid.

In the method for producing a nasal drop composition of the present invention, it is preferable that in the mixing step, an organic substance is further mixed together with the earthworm dung and the water.

In the method for producing a nasal drop composition of the present invention, the organic material is preferably wood.

The method for producing a nasal drop composition of the present invention preferably further comprises the following dilution step: the liquid obtained by recovering the water vaporized in the recovery step is diluted with water.

The nasal drop composition of the present invention is characterized by being obtained by the above-mentioned method for producing a nasal drop composition.

The nasal drop composition of the present invention is preferably used in the form of a mist.

The nasal drop composition of the present invention is preferably used for treating or preventing diseases or symptoms in the nasal cavity.

In the nasal drop composition of the present invention, the disease or symptom in the nasal cavity is preferably pollinosis or pyorrhea.

The method for treating pollinosis or empyema of the present invention is characterized by administering the nasal drop composition to a patient.

The nasal drop composition of the present invention is preferably used for treating pollinosis or pyorrhea.

ADVANTAGEOUS EFFECTS OF INVENTION

The present invention provides a method for producing a nasal drop composition suitable for treating or preventing diseases or symptoms in the nasal cavity, and a nasal drop composition obtained by the production method.

Detailed Description

The method for producing a nasal drop composition of the present invention is characterized by comprising the steps of: a mixing step of mixing the earthworms with water; and a recovery step of recovering the gasified water generated from the mixture obtained in the mixing step. In the mixing step, it is preferable that the organic material is further mixed with the earthworms' manure and the water. The detailed mechanism is not clear, and it is considered that since microorganisms such as bacteria contained in the earthworm dung decompose and ferment organic substances contained in the earthworm dung and organic substances separately mixed, a liquid obtained by recovering gasified water is effective for treating or preventing diseases or symptoms in the nasal cavity.

Hereinafter, the method for producing the nasal drop composition and the nasal drop composition of the present invention will be described in detail.

[ method for producing nose drops composition ]

(mixing Process)

The mixing step is a step of mixing the earthworms with water.

The soil of the earthworms is not particularly limited, and for example, red earthworms (lumbricis rubellus), LT earthworms (lumbricis terrestris), Eisenia foetida (Eisenia foetida), onyx anglica (allobophora caliginosa), Lumbricus octandra (dendriera octovalva octandra), oriental earthworms (allobophora japonica), octadona earthworms (Drawida hatamizu Hatai), black back earthworms (phermia digerms michaeelsen), common earthworms (phermia corniculata), earthworms (Pheretima agrostis), western girald earthworm (pheresis sibrella sieboldii), peri roughy (pheresis japonicas), and lagomorpha tuba (lagomorpha armeni), lagomorpha gigas (stemona), lagomorpha japonica (lagomorpha odorifera, and other earthworms (lagomorpha).

The water to be mixed with the earthworm dung is not particularly limited, and tap water or distilled water may be used. In addition, water may be purified by using a filter medium, a reverse osmosis membrane, or the like. From the viewpoint of removing microorganisms such as bacteria, a microporous filter material, a reverse osmosis membrane, or the like is preferably used, and for example, an SPG (white sand porous glass) permeable membrane is preferably used.

The mixing ratio of the earthworm dung to the water is preferably 0.05 to 20L, more preferably 0.1 to 10L, even more preferably 0.2 to 5L, and particularly preferably 0.5 to 2L, based on the earthworm dung of 1kg on average.

In the mixing step, it is preferable to further mix the organic matter with the earthworm dung and water. When an organic substance is mixed, a nasal drop composition having a more excellent effect of treating or preventing diseases or symptoms in the nasal cavity can be obtained, and the pH of the nasal drop composition can be adjusted. The organic matter is not particularly limited as long as it is decomposed by mixing with the earthworms' manure, and for example, organic matter derived from organisms such as animals, plants, fungi, and protozoa can be used. Examples of the plant-derived organic material include wood such as wood chips, sawdust, and rice hulls, and a fungal bed used for cultivation of mushrooms. When wood is mixed as an organic material, it is preferable because odorless liquid can be easily recovered in the recovery step. As the wood, wood chips are preferable. The wood species is not particularly limited, and may be needle-leaved trees such as yew, torreya, japanese cypress, hiba arborvitae, japanese cedar, pine, japanese yellow fir, spruce, fir, hemlock, redwood, etc., or broad-leaved trees such as cherry, oak, beech, birch, vertebra, maple, japanese alder, japanese basswood, japanese hornbeam, bamboo, etc. Among the woods, pine-mesh woods can be suitably used. More preferably, a wood of cypress family, still more preferably, a wood of decipieta sub-family, and particularly preferably, a wood of japanese cedar genus can be used.

The mixing ratio of the earthworm dung to the organic matter is preferably 0.05 to 20kg, more preferably 0.1 to 10kg, even more preferably 0.2 to 5kg, and particularly preferably 0.5 to 2kg, of the organic matter, based on the earthworm dung of 1kg on average.

The mixing method in the mixing step is not particularly limited, and it is preferable to sufficiently mix the mixture by stirring or the like. The order of mixing is not particularly limited, and for example, the earthworm dung and the organic substance may be added to a vessel and then water may be added thereto, or the organic substance and water may be added to a vessel and then the earthworm dung may be added thereto.

The total amount of the components does not need to be mixed in a centralized way at one time, and water, the excrement of the earthworms and/or organic matters can be continuously added and are mixed for a plurality of times. It is preferable to add the water to the reaction mixture continuously to recover the gasified water while supplementing the water reduced by evaporation, the soil and organic matters of earthworms reduced by decomposition.

Further, since fermentation is more stable over time from the initial mixing, if vaporized water is recovered after a lapse of, for example, about 1 day, a liquid having a more excellent effect of treating or preventing diseases or symptoms in the nasal cavity can be obtained. From this viewpoint, it is preferable to continuously add the water to continuously recover the vaporized water.

The mixture is preferably mixed with heating depending on the temperature, because heat is generated by fermentation of the earthworms' dung and the organic substances separately added. The temperature is increased, for example, to 30 to 50 ℃.

(recovering step)

The recovery step is a step of recovering water vaporized from the mixture obtained in the mixing step to obtain a liquid (hereinafter, also referred to as "organic substance decomposed water"). The recovery step may be performed while the mixing step is performed.

In the recovery step, the gasified water generated in the temperature range increased by the fermentation heat (reaction heat) generated by the fermentation of the mixture may be recovered, and it is not necessary to heat the water to the boiling point. Preferably, the mixing is carried out while heating depending on the temperature, for example, to 30 to 50 ℃.

The method of recovering is not particularly limited as long as the vaporized water can be recovered, and the vaporized water may be recovered by using, for example, a dehumidifier. As the dehumidifier, for example, a cooling type dehumidifier or a compression type dehumidifier can be used. Further, it is preferable to recover the vaporized water without boiling the mixture.

The method of making the recovered vaporized water into a liquid is not particularly limited, and if recovered using a dehumidifier, for example, organic matter-decomposed water can be obtained. The dehumidifier is not particularly limited as long as it can recover vaporized water in the form of a liquid, and, for example, a cooling type or compression type dehumidifier can be used.

The pH of the liquid obtained by recovering the gasified water is preferably 5 to 9, more preferably 6 to 8, and further preferably 6.5 to 7.5.

(dilution step)

The organic matter-decomposed water obtained by recovering the gasified water in the recovering step may be used as it is as a nasal drop composition, but it is preferably used by dilution. As the solvent for dilution, water may be used, and tap water or distilled water may be used. The water used for dilution may be purified by using a filter medium, a reverse osmosis membrane, or the like. From the viewpoint of removing microorganisms such as bacteria, a microporous filter material, a reverse osmosis membrane, or the like is preferably used, and for example, an SPG (white sand porous glass) permeable membrane is preferably used.

In the case of dilution, the dilution may be, for example, 1.5 to 10 times, preferably 4 to 6 times, and more preferably 4.5 to 5.5 times.

[ nose drop composition ]

The nasal drop composition of the present invention is characterized by being obtained by the method for producing a nasal drop composition of the present invention. The nasal drop composition of the present invention is obtained by the method for producing a nasal drop composition of the present invention, and is not particularly limited as long as it is effective for treating or preventing diseases or symptoms in the nasal cavity, and is preferably in a liquid state. Alternatively, the liquid nasal drop composition may be sprayed or evaporated into a mist for use. Further, the composition may be mixed with, for example, an excipient and processed into a solid such as a gel, a powder, or a granule.

The nasal drop composition of the present invention may contain a pharmaceutically acceptable carrier. The pharmaceutically acceptable carrier may be in the form of a conventional pharmaceutical preparation using an excipient, a binder, a disintegrating agent, a fluidizing agent, a lubricant, a coating agent, a suspending agent, a coloring agent, a sweetening agent, a surfactant, or the like, according to a known method. In addition, other therapeutic/prophylactic ingredients and pharmaceutically acceptable additives may be included.

The amount of the liquid recovered in the recovery step in the nasal drop composition of the present invention may be an amount effective for the purpose of blending. The appropriate amount of the liquid to be mixed depends on various factors such as the purpose, form, and production conditions, and in the case of a liquid nasal drop composition, for example, the amount of the liquid to be mixed recovered in the recovery step may be 5 vol% or more, preferably 5 to 50 vol%, more preferably 10 to 40 vol%, and still more preferably 20 to 30 vol%.

In addition, the nasal drop composition of the present invention may be used in an amount effective for the purpose. The appropriate amount depends on various factors such as the purpose, form, production conditions, and symptoms, and for example, when a liquid nasal drop composition is used in the nasal cavity by spraying it in a mist form, it is sufficient to spray it 6 to 10 times every 1 day.

The nasal drop composition of the present invention is preferably used for treating or preventing diseases or symptoms in the nasal cavity. The disease or symptom in the nasal cavity is not particularly limited, and examples thereof include pollinosis, empyema and the like.

Other active ingredients and known and conventional additives used in the nasal drop composition, such as coloring agents, perfumes, antioxidants, ultraviolet absorbers, chelating agents, surfactants, viscosity modifiers, pH modifiers, thickeners, antifoaming agents, preservatives, antibacterial/antimicrobial agents, dispersants, organic solvents, and the like, may be added to the nasal drop composition of the present invention within a range that does not impair the effects of the present invention.

In addition, the nasal drop composition of the present invention may contain dried powder, ground substance and/or extract of earthworm. By blending dry powder, ground matter and/or extract of earthworms, a liquid having a more excellent effect of treating or preventing diseases or symptoms in nasal cavities can be obtained. Among these, the extract of earthworms is more preferable in that more excellent effects can be obtained. As the earthworm extract, for example, an extract extracted from dried earthworm powder with water, ethanol, or an aqueous ethanol solution can be used.

The earthworms used as the raw material are not particularly limited, and for example, red earthworms (Lumbricis rubellus), LT earthworms (Lumbricis terrestris), Eisenia foetida (Eisenia foetida), Onychia anglica (Allobophora caliginosa), Esquirt earthworms (Dendrobaena octandra), Oriental earthworms (Allobophora japonica), Onychia earthworms (Drawida hattamizu Hatai), back black earthworms (Pheretima digergensis), common earthworms (Pheretima communis), field earthworms (Pheretima agrostis), Western girth earthworm (Pheretima globefield), and Hippodamia gigantea (Hibiscia gigantea), and post-emergence earthworms (Ocimula tuba) can be used.

In the present specification, the dry earthworm powder refers to a powder obtained by drying a ground product or extract of untreated or pretreated earthworms. The ground earthworm product refers to a liquid or paste-like product obtained by grinding untreated or pretreated earthworms. The earthworm extract is obtained by dissolving untreated or pretreated earthworms or ground earthworms in water or an organic solvent, and removing or separating insoluble components. The pretreatment is not particularly limited, and examples thereof include a removal treatment of a stain or the like described later. The dried powder, ground product, or extract of earthworms may be subjected to post-treatment, and examples of the post-treatment include granulation, filtration, purification, concentration, dilution, pH adjustment, and the like.

The method of grinding the ground earthworm products is not particularly limited, and the ground earthworm products may be ground using, for example, a homogenizer, a mixer, a homomixer, a pestle, a pressure cell disruptor, or the like.

The extraction method for obtaining the earthworm extract is not particularly limited, and for example, dried powder or ground earthworm can be dissolved in an extraction solvent, and insoluble components can be removed or separated and extracted. Examples of the extraction solvent include water, an aqueous solution, and organic solvents such as ethanol, acetone, and ethyl acetate, and 1 kind alone or 2 or more kinds in combination may be used. Among them, water, ethanol or an aqueous ethanol solution is preferable.

The drying method for obtaining the dried earthworm is not particularly limited, and drying can be performed by a drying method such as freeze drying, heat drying, and spray drying. Among them, freeze-drying is preferred for the reason described later.

It is preferable to remove digests remaining in the digestive tracts of earthworms, dirt adhering to the epidermis, and the like. The removal method is not particularly limited, and a known method can be used for removal. For example, the following methods may be used: a method of immersing living earthworms in an aqueous solution of an alkali salt such as a sodium salt or a potassium salt to excrete loess in a digestive tract (methods described in japanese patent application laid-open nos. 1-47718, 1-47719, 1-47720, and 1-268639); and a method of removing the soil in the digestive tract by leaving the living earthworms in an acid aqueous solution at 6 to 26 ℃ for 0.1 to 5 hours (the method described in Japanese patent application laid-open No. 3-72427).

As the removal method, it is preferable to bring chlorides of the following metals and/or hydroxycarboxylic acids into contact with earthworms.

The metal chloride is a chloride of at least 1 metal selected from the group consisting of potassium, sodium, magnesium and calcium. Namely, at least 1 selected from the group consisting of potassium chloride, sodium chloride, magnesium chloride and calcium chloride. Further, they may be a mixture thereof, or a mixture thereof with other harmless components which can be added to foods. Examples of such a mixture include salt, rock salt, and solar salt. The metal chloride can be used by sprinkling a powdery substance on live earthworms, thereby bringing the earthworms into contact with the metal chloride.

Preferably, after the chloride of the above metal is contacted with the live earthworm, the hydroxycarboxylic acid is contacted with the live earthworm as described below. Alternatively, the hydroxycarboxylic acid may be contacted with the earthworm as described below without contacting with the chloride of the metal.

The contact with the hydroxycarboxylic acid may be performed by sprinkling powdery hydroxycarboxylic acid on live earthworms. Alternatively, the substrate may be immersed in an aqueous hydroxycarboxylic acid solution having a pH of 2 to 5. When the contact with the hydroxycarboxylic acid is carried out after the contact with the metal chloride, the contact with the hydroxycarboxylic acid is preferably carried out immediately after the contact with the metal chloride. Further, it is preferable that the earthworms are washed with water before the live earthworms are brought into contact with the hydroxycarboxylic acid. When the earthworms are brought into contact with hydroxycarboxylic acids after removing chlorides of the metals by washing with water, dried earthworm powder having high enzymatic activity can be obtained. When the washing with water is performed before the contact with the hydroxycarboxylic acid, the washing with water is preferably performed within 30 minutes, more preferably within 20 minutes after the contact with the metal chloride is started. The washing method is not particularly limited, and a known method can be used.

If live earthworms are brought into contact with hydroxycarboxylic acid powder for a long time, the earthworms die to lose life functions and cannot excrete digesta in the digestive tract, and therefore, it is preferable to dilute the hydroxycarboxylic acid with water as soon as possible within preferably 30 seconds or less, more preferably within 20 seconds, and adjust the pH to a range of 2 to 5.

Since hydroxycarboxylic acids form an uncomfortable living environment for earthworms, live earthworms release body fluids and excretions due to their self-protective nature to improve the living environment. Since hydroxycarboxylic acids have bactericidal properties, they exhibit the action of promoting excretion of digesta and the like remaining in the digestor as described above, and are expected to have an effect of sterilizing various bacteria attached to earthworms.

The crystalline hydroxycarboxylic acid used in the above method can be used regardless of the number of hydroxyl groups or the number of carboxyl groups if it is crystalline under the conditions of use. That is, any of monohydroxymonocarboxylic acids, monohydroxypolycarboxylic acids, polyhydroxymonocarboxylic acids, and polyhydroxypolycarboxylic acids can be used.

Examples of the hydroxycarboxylic acid include glycolic acid, lactic acid, acetic acid, β -hydroxypropionic acid, α -hydroxybutyric acid, β -hydroxybutyric acid, α -hydroxyn-valeric acid, β -hydroxyn-valeric acid, malic acid, α -methylmalic acid, α -hydroxyglutaric acid, β -hydroxyglutaric acid, citric acid, malonic acid, and succinic acid. Among them, lactic acid, acetic acid, malic acid, citric acid, malonic acid and succinic acid are preferable from the viewpoint of availability and ready availability to food. The hydroxycarboxylic acid may be used alone in 1 kind, or may be used in combination of 2 or more kinds.

The moisture content of the tissue of live earthworms is 65%. The time for operating the life-saving function of live earthworms is somewhat redundant, but since enzymes work when live earthworms die, the time control must be carefully performed when the earthworms are left in an uncomfortable living environment. This time is generally within the range of 3 to 180 minutes, depending on the conditions.

The earthworm bodies treated with the hydroxycarboxylic acid are preferably washed with water and ground to prepare a ground product in a liquid or paste form. The cleaning is preferably carried out with pure water. The washing method is not particularly limited, and a known washing method can be used. The total time of the treatment steps before the grinding, that is, the time from when the live earthworms are sprinkled with the metal chloride to when the washing with water of the hydroxycarboxylic acid is completed, is preferably 240 minutes or less in total.

The above-mentioned grinding method is not particularly limited, and is usually carried out at 1 to 25 ℃ using, for example, a homogenizer, a mixer, a homomixer, a kneader, or a pressure cell disruptor. From the viewpoint of suppressing decomposition of the earthworm-constituting components, the reaction is preferably carried out at a low temperature, preferably at a temperature of 2 to 15 ℃.

The ground product obtained by grinding earthworms is stored in, for example, a stainless tray, and freeze-dried. In this case, although the enzyme contained in the earthworm organism does not act on live cells, it acts on dead cells instantaneously, so that there is a concern that putrefactive gas is generated, and in order to prevent this, it is preferable to perform rapid cooling/freezing to-18 ℃ to-35 ℃ instantaneously, suppress the action of the enzyme, and then perform freeze-drying.

As described above, rapid freezing is preferred in order to pulverize earthworms without impairing their original pharmacological effects, while too short a time of freezing is not preferred because impurities present together with proteins, which are the main components of the earthworm paste, form spot-like non-frozen portions and cannot be separated in some cases. Therefore, the freezing is preferably performed at a low temperature of-18 ℃ to-35 ℃ for 20 to 240 hours, more preferably 50 to 170 hours.

In freeze drying, it is important to select conditions that can remove moisture together with impurities without leaving residues. Therefore, it is preferable to control the temperature within a range of 10 to 60 hours while raising the temperature stepwise within a temperature range of-60 ℃ to +90 ℃ under a pressure of 50Pa or less.

As a method of freeze-drying, for example, as described above, the ground material is frozen at a temperature of-18 ℃ to-35 ℃ for 20 to 240 hours, then heated in stages at a temperature of-60 ℃ to +90 ℃ and vacuum-dried in stages at a pressure of 25 to 40Pa for 10 to 60 hours, whereby a pale yellow dry earthworm powder in a sterile state can be obtained.

Further, it is preferable to include: and a step of dissolving the material obtained by freeze-drying the ground material in water or an aqueous ethanol solution to remove or separate insoluble components. The step of removing or separating the insoluble component may be carried out by settling, centrifugation, filtration, or the like by standing in the same manner as described above. The step of dissolving in water or an aqueous ethanol solution is preferably performed while stirring or shaking. The time required for dissolving in water is preferably 1 to 120 minutes, more preferably 5 to 80 minutes. The ethanol concentration of the ethanol aqueous solution is not particularly limited, but is preferably 10 to 70% (v/v), more preferably 30 to 60%.

The earthworm extract may be used in the form of an aqueous solution of the supernatant obtained by dissolving the earthworm extract in water or an aqueous ethanol solution as described above, or may be used in the form of a concentrate obtained by evaporating water or may be used in the form of a powder obtained by drying the supernatant. Or drying the supernatant to obtain powder, and dissolving in water. Alternatively, the freeze-dried earthworm paste may be used as it is without dissolving the earthworm paste in water or an aqueous ethanol solution.

In addition, as a removing method, it is preferable to perform: before the live earthworms are treated in an uncomfortable environment, namely before the live earthworms contact metal chloride or hydroxycarboxylic acid, the live earthworms are moved into a flat box such as a bread box and placed in a bright place for 10 to 50 hours to remove dirt attached to the epidermis. The time for placing the film in a bright place is more preferably 12 to 24 hours. The amount of earthworms stored in this case is preferably about 30 to 60mm, preferably about 40 to 50mm, thick. In the flat box, since there are no foreign matters such as sand and mud, and there is a fear that earthworms are nocturnal and live activities in a dark place, and physical strength is consumed, it is preferable to keep brightness by an electro-optical culture method or the like at night. The live earthworms exert their self-defense instinct by the treatment, excrete the remaining digesta in the digestive tract, cover the whole body with the excreta, prevent water from evaporating, and maintain the living environment, so that the digesta in the digestive tract and the excreta attached to the epidermis can be finally removed by repeatedly peeling off the covered excreta by an appropriate means.

The removal of the soil adhering to the surface of the earthworms can be performed by, for example, covering the live earthworms with a nonwoven fabric and adsorbing the soil thereon. By combining the setting of the bright place and the removal of the dirt adhering to the surface with the contact with the metal chloride and/or hydroxycarboxylic acid, further discharge and removal of the toxic substance in the earthworm body can be expected.

As a method for obtaining the dried earthworm powder, the following method is preferred, particularly from the viewpoint of storage stability of the dried powder.

(A-1) A method for producing a dry earthworm powder, comprising the steps of:

bringing live earthworms into contact with a chloride of at least 1 metal selected from the group consisting of potassium, sodium, magnesium and calcium,

and then, bringing the powdery hydroxycarboxylic acid into contact with live earthworms, diluting the mixture with water to adjust the pH to 2 to 5, holding the mixture for 3 to 180 minutes, washing the live earthworms with water, grinding the washed and ground earthworms, and freeze-drying the ground earthworms.

(A-2) A method for producing a dry earthworm powder, comprising the steps of:

then, immersing live earthworms in an aqueous solution of hydroxycarboxylic acid adjusted to a pH of 2-5, holding the solution for 3-180 minutes, washing the live earthworms with water, grinding the washed and ground earthworms, and freeze-drying the ground earthworms.

(A-3) A method for producing a dry earthworm powder, comprising the steps of: the ground product of the above (A-1) or (A-2) is freeze-dried, and the resultant is dissolved in water or an aqueous ethanol solution, and after removing or separating the insoluble fraction, further freeze-dried.

Further, after freeze-drying the ground material obtained by grinding live earthworms, the obtained dried material may be subjected to a heat treatment in view of sterilization of the dried material. The temperature of the heat treatment is preferably 110 ℃ or higher and less than 130 ℃. If the heating temperature is less than 110 ℃, the sterilization of the dried product may be insufficient, and if the temperature is 130 ℃ or higher, the enzyme contained in the dried earthworm product is inactivated and the activity is lowered, which is not preferable. More preferably 115 to 125 ℃. The heating method is not particularly limited, and examples thereof include a method of blowing hot air, a method of using a heating jacket, a method of heating with a heater mounted on a tray or the like, and a method of using a constant temperature thermostat. The heating time is preferably 30 seconds to 130 minutes, more preferably 30 minutes to 90 minutes, and further preferably 60 minutes to 90 minutes. If the heating time is too short, sterilization may be insufficient, and if it is too long, the activity of the enzyme may be inactivated, which is not preferable. Since the enzyme activity is inactivated when the enzyme in the liquid is subjected to the above-mentioned heat treatment, it is preferable to heat-treat the dried earthworm powder.

The following method is preferred as a method for obtaining ground earthworms.

(B-1) A method for producing ground earthworm products, comprising the steps of:

then, the powdery hydroxycarboxylic acid is brought into contact with the live earthworms, the mixture is diluted with water to adjust the pH to 2 to 5, the mixture is kept for 3 to 180 minutes, and then the live earthworms are washed with water and ground.

(B-2) A method for producing ground earthworm products, comprising the steps of:

contacting live earthworms with a chloride of a metal selected from the group consisting of potassium, sodium, magnesium and calcium,

then, the live earthworms are immersed in an aqueous hydroxycarboxylic acid solution adjusted to a pH of 2 to 5, kept for 3 to 180 minutes, washed with water, and ground.

The following method is preferred as a method for obtaining the earthworm extract.

(C-1) A method for producing an earthworm extract, comprising the steps of:

contacting live earthworms with a chloride of at least 1 metal selected from the group consisting of potassium, sodium, magnesium and calcium,

then, the powdery hydroxycarboxylic acid is brought into contact with live earthworms, the pH is adjusted to 2 to 5 by dilution with water, the mixture is kept for 3 to 180 minutes, the live earthworms are washed with water and ground, the ground product obtained by freeze-drying the ground product is dissolved in water or an aqueous ethanol solution, and insoluble components are removed or separated.

(C-2) A method for producing an earthworm extract, comprising the steps of:

then, the live earthworms are immersed in an aqueous hydroxycarboxylic acid solution adjusted to a pH of 2 to 5, and after 3 to 180 minutes, the live earthworms are washed with water, ground, and the resulting ground product is freeze-dried to obtain a substance which is dissolved in an aqueous water or ethanol solution, and insoluble components are removed or separated.

The amount of the earthworm dry powder, ground product and/or extract to be blended is not particularly limited, and in the case of a liquid oral composition, the nasal drop composition is, for example, 0.1 to 100mg, preferably 0.2 to 50mg, and more preferably 1 to 20mg, per 100L of the total dry mass of the extract extracted with water.

Examples

The present invention will be described in further detail below with reference to examples. The present invention is not limited in any way by the following examples. In the following "%" unless otherwise specified, they are all based on mass.

(organic decomposition Water)

40L of water was added to a reaction tank containing 40kg of red earthworm-containing soil and 15kg of wood (Japanese cedar) (10 kg of a blend of 5kg of wood chips, sawdust and rice hulls), and the gasified water was recovered by a dehumidifier (DM-30, manufactured by NAKATOMI) installed in the reaction tank over about 1 day while stirring, to obtain 15 to 20L of liquid. Further, 10 to 20L of water and 10kg of wood (Japanese cedar) (5 kg of a blend of 5kg of wood chips, sawdust and rice hulls) were added to the reaction tank, and the gasified water was recovered by a dehumidifier in the same manner in about 1 day to obtain 15 to 20L of liquid. About 40L of a mixed liquid (pH8.71) was obtained as organic matter-decomposed water.

In the production of the organic matter-decomposed water, tap water was purified with an SPG (white sand porous glass) permeable membrane (SPG Techno) and activated carbon.

(earthworm extract)

After 24 hours of storage in a bright place, 30kg of live red earthworms from which dirt adhering to the skin was removed were spread in a thickness of about 5cm in a dish, and 250g of sodium chloride was uniformly scattered thereon. After 20 minutes, the earthworms were washed with water. Thereafter, 250g of citric acid was similarly scattered, and then, 30 liters of pure water was added for 15 seconds to dilute the solution. At this time, the pH immediately after the addition of water was 2.25, and the pH at the time of complete dilution was 2.74. When the citric acid powder is scattered, the earthworms release yellow body fluid at one stroke. After dilution with water, the mixture was kept in this state for 20 minutes. Subsequently, live earthworms were taken out from the dirty aqueous citric acid solution, washed with water, and then ground at 10 ℃ using a homogenizer to prepare an earthworm paste. Next, the earthworm paste was degassed by suction to remove gas contained therein, transferred to a stainless steel tray, immediately cooled to-35 ℃ rapidly, maintained at that temperature for 50 hours, and slowly frozen. The freeze-dried earthworm paste was vacuum freeze-dried by keeping the frozen earthworm paste at-35 ℃ under a pressure of 0Pa for 2 hours, then raising the temperature to 25 ℃, drying at 40Pa for 10 hours, then drying at 40 ℃ under a pressure of 35Pa for 14 hours, then drying at 65 ℃ under a pressure of 35Pa for 12 hours, and finally keeping the temperature at 80 ℃ under a pressure of 25Pa for 6 hours. This treatment gave a pale yellow dry earthworm powder having a water content of 8 mass%.

25g of the dry earthworm powder obtained above was collected, and 500mL of distilled water was added thereto, followed by extraction with stirring at room temperature for 1 hour. Centrifuging the obtained extractive solution (10000 Xg, 4 deg.C, 15 min), and collecting supernatant to obtain Lumbricus extract. Micronizing the obtained extract with a freeze vacuum drier, collecting 30mg, and diluting with 500mL of distilled water to obtain diluted stock solution.

(nasal drop composition)

The organic substance decomposition water obtained above was diluted 5 times with water. As water used for dilution, water obtained by purifying tap water with an SPG (white sand porous glass) permeable membrane (manufactured by SPG Techno) and activated carbon was used.

Mixing the diluted organic decomposition water and the obtained diluted stock solution of the earthworm extract in a ratio of 1000: 1, and passed through a 0.2 μm filter to obtain a nasal drop composition.

Example 1

< pollinosis 1>

The nasal drop composition obtained above was applied to the following subjects in a mist form in the nasal cavity, and the symptoms of pollinosis were observed for 6 to 10 times per day, 1 time per day, and 2 to 5 times per 1 time with a commercially available nasal cavity washing nebulizer.

The tested person: female, 22 years old: 2 years medical record

After 3 days, nasal discharge and tear water were reduced

After 7 days, the nasal discharge and the lacrimal fluid stop

After 15 days, the pain of pollinosis is relieved

After 30 days, the disease is healed

Example 2

< pollinosis 2>

The tested person: male, 52 years old: 15 years of medical history

After 7 days, the nasal discharge and the itching of the eyes are reduced

After 14 days, the nasal discharge and itching disappeared

After 25 days, symptoms improved

After 30 days, the symptoms disappeared

Example 3

< pus accumulation syndrome 1>

The following subjects who sprayed the nasal drop composition obtained as described above with a commercially available nasal wash sprayer 2 to 5 drops per day, 6 to 10 times per 1 day, 1 time per 1 time were observed for symptoms of empyema.

The tested person: male, 18 years old: the patient has a history of 2 months and has yellow nasal discharge

After 7 days, the amount and color of nasal discharge decreased

After 10 days, the nasal discharge stopped

After 25 days, the nose is opened and the patient can be healed

Example 4

< pus accumulation syndrome 2>

The tested person: female, 15 years old: 1 month of medical history

After 3 days, nasal discharge decreased

After 7 days, the nasal discharge stopped

After 15 days, the nose is opened and the patient can be healed

As shown in examples 1 to 4, it is known that a nasal drop composition suitable for treating or preventing diseases or symptoms in the oral cavity can be produced by recovering water produced by vaporization of a mixture of earthworm dung and water.

Claims

1. A method for producing a nasal drop composition for treating or preventing rhinorrhea caused by pollinosis or pyorrhea, comprising the steps of: a mixing step of mixing the earthworms with soil, water and wood; and a recovery step of recovering the vaporized water generated from the mixture obtained in the mixing step to obtain a liquid,

in the mixing procedure, wood, earthworm dung and water are mixed together, and the mixing ratio of the earthworm dung to the water is as follows: the average amount of the earthworm dung soil is 1kg, the amount of water is 0.05-20L, and the mixing ratio of the earthworm dung soil to wood is as follows: the average amount of the earthworms is 1kg, the amount of the wood is 0.05-20 kg,

the nasal drop composition contains 5-50 vol% of a liquid obtained by recovering water vaporized in the recovery step; and 0.1 to 100mg of earthworm extract in 100L of the nose drop composition on average in terms of dry mass of the extract extracted with water,

the earthworm extract is obtained by a method for preparing the earthworm extract, which comprises the following steps:

then, contacting the powdery hydroxycarboxylic acid with live earthworms, diluting the mixture with water to adjust the pH to 2-5, and keeping the pH for 3-180 minutes; or immersing live earthworms in a hydroxycarboxylic acid aqueous solution adjusted to a pH of 2-5, keeping for 3-180 minutes,

washing live Lumbricus with water, grinding, freeze drying to obtain a substance, dissolving in water, and removing or separating insoluble components.

2. The method for producing a nasal drop composition according to claim 1, further comprising a dilution step of diluting with water the liquid obtained by recovering the water vaporized in the recovery step.

3. The method for producing a nasal drop composition according to claim 1, wherein the nasal drop composition is a nasal drop composition to be used by spraying or evaporating into a mist.

4. The method for producing a nasal drop composition according to claim 2, wherein the nasal drop composition is a nasal drop composition to be used by spraying or evaporating into a mist.