CN109917122A - A kind of chemiluminescence detecting - Google Patents
A kind of chemiluminescence detecting Download PDFInfo
- Publication number
- CN109917122A CN109917122A CN201910173490.8A CN201910173490A CN109917122A CN 109917122 A CN109917122 A CN 109917122A CN 201910173490 A CN201910173490 A CN 201910173490A CN 109917122 A CN109917122 A CN 109917122A
- Authority
- CN
- China
- Prior art keywords
- hole
- cone
- reaction cup
- liquid
- chemiluminescence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The present invention proposes a kind of chemiluminescence detecting, including the light detection module, cone, liquid-feeding tube, reaction cup, light detection module is equipped with optical channel, light detection module is fixedly connected with cone, one end of the cone is arranged in reaction cup, cone is equipped with first through hole, first through hole and optical channel are conducted, cone is equipped with the second through-hole, second through-hole and first through hole form the angle of an acute angle, liquid-feeding tube is obliquely fixed on cone through the second through-hole, reagent solution can be added in the reaction cup by the liquid-feeding tube, chemiluminescence can occur in the reaction cup, the light issued enters optical channel through the first through hole and is detected by the light detection module.Chemiluminescence detecting structure of the invention is simple, small in size, at low cost, has the characteristics that miniaturization, realizes and detects chemiluminescence while liquid feeding, to adapt to the detection of flash type chemoluminescence method, while can be adapted for disposable reaction cup and reagent strip.
Description
Technical field
The present invention relates to chemiluminescence immunoassay technology field, especially a kind of chemiluminescence detecting.
Background technique
POCT quickly examines (point-of-care testing), is the clinical detection carried out beside patient, is adopting
Sample scene is analyzed at once, is saved complex process program of the sample in laboratory inspection, is quickly obtained the one of inspection result
Class method.POCT have many advantages, such as to be quickly obtained testing result, it is easy to operate, be easy to use and save the cost, in the day of people
Often increasing effect is played in life.
The technology that POCT is related to is varied, in brief, can be divided into simple colour developing, enzyme label, immunoassay, electrification
Learn detection etc..The wherein specificity of the existing immune response of chemiluminescence immune assay, and have chemiluminescent high sensitivity, can be with
Greatly extend the detection range of common immunoassays method.
POCT chemical illumination immunity analysis instrument currently on the market, after needing that various reagents reaction is added in reaction cup,
Reaction cup is moved to detection position again and carries out the detection that shines, the detection that shines cannot be carried out while injecting reagent.Therefore only
Suitable for wide variety of glow-type chemiluminescence principle, it is not suitable for flash type chemiluminescence principle, and is shone with acridinium ester as the sudden strain of a muscle of representative
Light type chemiluminescence is the chemiluminescence principle of mainstream currently on the market.
Summary of the invention
To solve the above-mentioned problems, the present invention proposes that one kind had not only minimized but also can detect position to reaction cup shining
The chemiluminescence detecting of middle injection reagent, to adapt to the detection of flash type chemoluminescence method.
What the invention is realized by the following technical scheme:
The present invention proposes a kind of chemiluminescence detecting, including the light detection module, cone, liquid-feeding tube, reaction
Cup, the light detection module are equipped with optical channel, and the light detection module is fixedly connected with the cone, the reaction cup setting
In one end of the cone, the cone is equipped with first through hole, and the first through hole and the optical channel are conducted, described
Cone is equipped with the second through-hole, and second through-hole and the first through hole form the angle of an acute angle, and the liquid-feeding tube passes through
Second through-hole is worn to obliquely be fixed on the cone, reagent solution can be added to described by the liquid-feeding tube
In reaction cup, chemiluminescence can occur in the reaction cup, the light issued enters optical channel by institute through the first through hole
State the detection of light detection module.
Further, the cone includes first end face and second end face, and the cone passes through first end face and institute
It states light detection module to be fixedly connected, the reaction cup is contacted with the second end face.
Further, the liquid-feeding tube is fixed in second through-hole by lighttight adhesive.
Further, the liquid-feeding tube prolongs the inner wall of the liquid-feeding tube relative to the tilt angle of the cone
Long line protrudes into the reaction cup, and does not intersect with the wall of cup of the reaction cup.
Further, the cone includes outer wall, and second through-hole is connected to the first through hole and the outer wall.
Further, the first through hole is connected to the first end face and the second end face.
Further, the second end face is equipped with recessed portion, and the reaction cup includes alar part and along the alar part end court
The lug boss of upper extension, the lug boss are embedded in the recessed portion and make second end face described in the wing contacts.
Further, the light detection module includes tripper and photomultiplier tube, described in the tripper control
The light of optical channel enters the photomultiplier tube.
Further, the material of the reaction cup is light-proof material.
Further, the chemiluminescence detecting further includes reagent strip, and the reaction cup is arranged in the reagent strip
On.
Beneficial effects of the present invention:
Chemiluminescence detecting proposed by the present invention, including the light detection module, cone, liquid-feeding tube, reaction cup,
The light detection module is equipped with optical channel, and the light detection module is fixedly connected with the cone, and the reaction cup setting exists
One end of the cone, the cone are equipped with first through hole, and the first through hole and the optical channel are conducted, the screening
Light cylinder is equipped with the second through-hole, and second through-hole and the first through hole form the angle of an acute angle, and the liquid-feeding tube runs through
To obliquely be fixed on the cone, reagent solution can be added to described anti-second through-hole by the liquid-feeding tube
It answers in cup, chemiluminescence can occur in the reaction cup, it is described that the light issued through the first through hole enters optical channel
The detection of light detection module.Chemiluminescence detecting structure of the invention is simple, small in size, at low cost, has the characteristics that miniaturization,
It realizes and detects chemiluminescence while liquid feeding, to adapt to the detection of flash type chemoluminescence method, while can be adapted for primary
The reaction cup and reagent strip of property.
Detailed description of the invention
Fig. 1 is the structural profile illustration of chemiluminescence detecting of the invention；
Fig. 2 is the partial structurtes section enlarged diagram of chemiluminescence detecting of the invention.
Specific embodiment
In order to it is clearer, completely illustrate technical solution of the present invention, the present invention is made with reference to the accompanying drawing further
Explanation.
Fig. 1-Fig. 2 is please referred to, the present invention proposes a kind of chemiluminescence detecting, including the light detection module 1, shading
Cylinder 2, liquid-feeding tube 3, reaction cup 4, the light detection module 1 are equipped with optical channel 13, the light detection module 1 and the cone 2
It being fixedly connected, the reaction cup 4 is arranged in one end of the cone 2, and the cone 2 is equipped with first through hole 201, and described the
One through-hole 201 and the optical channel 13 are conducted, and the cone 2 is equipped with the second through-hole 204, second through-hole 204 and institute
The angle that first through hole 201 forms an acute angle is stated, the liquid-feeding tube 3 is through second through-hole 204 to obliquely fix
In on the cone 2, reagent solution can be added in the reaction cup 4 by the liquid-feeding tube 3, can in the reaction cup 4
Chemiluminescence occurs, the light issued enters optical channel 13 through the first through hole 201 and is detected by the light detection module 1.
Further, the cone 2 includes first end face 202 and second end face 203, and the cone 2 passes through first
End face 202 is fixedly connected with the light detection module 1, and the reaction cup 4 is contacted with the second end face 203.
In the present embodiment, the first through hole 201 is vertical core, and second through-hole 204 is inclined hole, and described first is logical
Angle between hole 201 and second through-hole 204 is tilt angle of the liquid-feeding tube 3 relative to the cone 2.
Further, the liquid-feeding tube 3 is fixed in second through-hole 204 by lighttight adhesive.
In the present embodiment, solid by lighttight adhesive between the liquid-feeding tube 3 and second through-hole 204
It is fixed, first through hole 201 is entered by the gap between the liquid-feeding tube 3 and second through-hole 204 to avoid external stray light
And then detected by light detection module 1, influence the result of detection.
Further, the liquid-feeding tube 3 makes the inner wall of the liquid-feeding tube 3 relative to the tilt angle of the cone 2
301 extended line 302 protrudes into the reaction cup 4, and does not intersect with the wall of cup 401 of the reaction cup 4, so that passes through institute
The reagent solution for stating the addition of liquid-feeding tube 3 is just fallen into the reaction cup 4, prevents reagent droplet from falling in the wall of cup of the reaction cup 4
401 splash down the gap between the reaction cup 4 and the first through hole 201 in turn, prevent contaminated equipment.
In the present embodiment, the inside diameter ranges of the liquid-feeding tube 3 are between 0.1mm~1.6mm, which can be with
Reagent droplet when 3 liquid feeding of liquid-feeding tube is avoided to be adhered to the nozzle of the liquid-feeding tube 3.
In the present embodiment, the external infusion equipment of the liquid-feeding tube 3 is used for liquid feeding, and the liquid-feeding tube 3 is using opaque
Material is made, and such as black ABS, black polypropylene etc. enters described first by the liquid-feeding tube 3 to avoid extraneous stray light
Through-hole 201.
Further, the cone 2 includes outer wall 205, and second through-hole 204 is connected to 201 He of first through hole
The outer wall 205.
Further, the first through hole 201 is connected to the first end face 202 and the second end face 203.
In the present embodiment, the cone 2 passes through the first end face 202 and the fixed company of the light detection module 1
It connects, the second end face 203 and the reaction cup 4 contact.
Further, the second end face 203 is equipped with recessed portion 2031, and the reaction cup 4 is including alar part 403 and along described
The lug boss 402 that 403 endways of alar part extend, the lug boss 402 are embedded in the recessed portion 2031 and make the wing
Portion 403 contacts the second end face 203.
In the present embodiment, it may be implemented by the cooperation of the recessed portion 2031 and the lug boss 402 described anti-
Cup 4 is answered quickly to be mounted on the cone 2, and easy to operate, fix stably；The lug boss 402 is embedded in the recess
In portion 2031 and the alar part 403 is made to contact the second end face 203, so that the alar part 403 covers the reaction
Gap between cup 4 and the first through hole 201, prevents ambient light from entering first through hole 201 by the gap and is examined by light
It surveys module 1 to detect, influences the result of detection.Mutual chimeric pass through of the recessed portion 2031 and the lug boss 402 can also
So that cooperating closely between the reaction cup 4 and the first through hole 201, prevent ambient light from entering interference detection results.
Further, the light detection module 1 includes tripper 11 and photomultiplier tube 12, and the tripper 11 is controlled
The light for making the optical channel 13 enters the photomultiplier tube 12.
In the present embodiment, tripper 11 can execute switch motion, start when making, the light of the optical channel 13 into
Enter the photomultiplier tube 12；When pass acts, the optical channel 13 is capped, and the light of the optical channel 13 cannot be introduced into photoelectricity times
Increase pipe 12, the tripper 11 is set to close optical path when not needing measurement chemiluminescence and enters light to avoid ambient light
Electric multiplier tube 12.
Further, the material of the reaction cup 4 is light-proof material.
In the present embodiment, the reaction cup 4 is made of light-proof material, such as black ABS, black polypropylene etc.,
Enter light detection module 1 to avoid extraneous stray light.The reaction cup 4 is an integral molding structure.
Further, the chemiluminescence detecting further includes reagent strip 5, and the reaction cup 4 is arranged in the reagent
On item 5.
In the present embodiment, chemiluminescence detecting structure of the invention is simple, small in size, at low cost, has small
Type feature realizes and detects chemiluminescence while liquid feeding, to adapt to the detection of flash type chemoluminescence method, while can fit
For disposable reaction cup 4 and reagent strip.
The use step for demonstrating chemiluminescence detecting of the invention is exemplified below, by taking acridinium ester shines as an example,
Its testing process that shines is as follows:
1) sample, acridinium ester label antibody, the coated magnetic particle of antibody are heated in reaction cup 4 together and is incubated for.
2) magnetic particle is adsorbed on to 301 bottom of inner wall of reaction cup 4 with magnet close to 4 bottom of reaction cup, by remaining fluid
Body discharge.
3) cleaning solution is added in reaction cup 4 and cleans magnetic bead, be then discharged out liquid；Repeatedly.
4) hydrogenperoxide steam generator is heated in reaction cup 4, and is mixed with magnetic particle.
5) reaction cup 4 is fixed on to the second end face 203 of cone 2, the tripper 11 of light detection module 1 opens optical path
Start to measure, sodium hydroxide solution is then injected to reaction cup 4 by liquid-feeding tube 3, mixed solution starts chemiluminescence, 5s or so
The tripper 11 of reflective end, light detection module 11 closes optical path, postread number of photons, output test result.
Certainly, the present invention can also have other numerous embodiments, be based on present embodiment, those skilled in the art
Obtained other embodiments under the premise of not making any creative work belong to the range that the present invention is protected.
Claims (10)
1. a kind of chemiluminescence detecting, which is characterized in that including the light detection module, cone, liquid-feeding tube, reaction
Cup, the light detection module are equipped with optical channel, and the light detection module is fixedly connected with the cone, the reaction cup setting
In one end of the cone, the cone is equipped with first through hole, and the first through hole and the optical channel are conducted, described
Cone is equipped with the second through-hole, and second through-hole and the first through hole form the angle of an acute angle, and the liquid-feeding tube passes through
Second through-hole is worn to obliquely be fixed on the cone, reagent solution can be added to described by the liquid-feeding tube
In reaction cup, chemiluminescence can occur in the reaction cup, the light issued enters optical channel by institute through the first through hole
State the detection of light detection module.
2. chemiluminescence detecting according to claim 1, which is characterized in that the cone include first end face and
Second end face, the cone are fixedly connected by first end face with the light detection module, the reaction cup and described second
End face contact.
3. chemiluminescence detecting according to claim 1, which is characterized in that the liquid-feeding tube passes through lighttight glue
Stick is fixed in second through-hole.
4. chemiluminescence detecting according to claim 1, which is characterized in that the liquid-feeding tube is relative to the shading
The tilt angle of cylinder protrudes into the extended line of the inner wall of the liquid-feeding tube in the reaction cup, and the not cup with the reaction cup
Wall intersection.
5. chemiluminescence detecting according to claim 1, which is characterized in that the cone includes outer wall, described
Second through-hole is connected to the first through hole and the outer wall.
6. chemiluminescence detecting according to claim 1, which is characterized in that the first through hole connection described first
End face and the second end face.
7. chemiluminescence detecting according to claim 6, which is characterized in that the second end face is equipped with recessed portion,
The reaction cup includes that alar part and the lug boss along alar part endways extension, the lug boss are embedded in the recessed portion
And make second end face described in the wing contacts.
8. chemiluminescence detecting according to claim 1, which is characterized in that the light detection module includes fast door machine
Structure and photomultiplier tube, the light that the tripper controls the optical channel enter the photomultiplier tube.
9. chemiluminescence detecting according to claim 1, which is characterized in that the material of the reaction cup is opaque
Material.
10. chemiluminescence detecting according to claim 1, which is characterized in that the chemiluminescence detecting is also
Including reagent strip, the reaction cup is arranged on the reagent strip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910173490.8A CN109917122B (en) | 2019-03-07 | 2019-03-07 | Chemiluminescence detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910173490.8A CN109917122B (en) | 2019-03-07 | 2019-03-07 | Chemiluminescence detection device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109917122A true CN109917122A (en) | 2019-06-21 |
| CN109917122B CN109917122B (en) | 2022-04-01 |
Family
ID=66963837
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910173490.8A Active CN109917122B (en) | 2019-03-07 | 2019-03-07 | Chemiluminescence detection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109917122B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205139019U (en) * | 2015-11-13 | 2016-04-06 | 深圳市帝迈生物技术有限公司 | Miniaturized whole blood immunity measuring device |
| CN205786331U (en) * | 2016-05-29 | 2016-12-07 | 深圳市生强科技有限公司 | The detection device of chemically based luminescence method |
| CN107192831A (en) * | 2017-05-24 | 2017-09-22 | 青岛科技大学 | A kind of method that chemiluminescence detects glycosylated hemoglobin |
| CN207248880U (en) * | 2017-07-18 | 2018-04-17 | 深圳沃德生命科技有限公司 | Measure the reagent strip and device of thrombus elastic force |
| US20180119200A1 (en) * | 2016-11-01 | 2018-05-03 | Promega Corporation | Coelenterazine analogues tethered to energy acceptors |
| CN207408309U (en) * | 2017-10-26 | 2018-05-25 | 苏州立禾生物医学工程有限公司 | A kind of chemiluminescence detector shines measuring chamber |
-
2019
- 2019-03-07 CN CN201910173490.8A patent/CN109917122B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205139019U (en) * | 2015-11-13 | 2016-04-06 | 深圳市帝迈生物技术有限公司 | Miniaturized whole blood immunity measuring device |
| CN205786331U (en) * | 2016-05-29 | 2016-12-07 | 深圳市生强科技有限公司 | The detection device of chemically based luminescence method |
| US20180119200A1 (en) * | 2016-11-01 | 2018-05-03 | Promega Corporation | Coelenterazine analogues tethered to energy acceptors |
| CN107192831A (en) * | 2017-05-24 | 2017-09-22 | 青岛科技大学 | A kind of method that chemiluminescence detects glycosylated hemoglobin |
| CN207248880U (en) * | 2017-07-18 | 2018-04-17 | 深圳沃德生命科技有限公司 | Measure the reagent strip and device of thrombus elastic force |
| CN207408309U (en) * | 2017-10-26 | 2018-05-25 | 苏州立禾生物医学工程有限公司 | A kind of chemiluminescence detector shines measuring chamber |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109917122B (en) | 2022-04-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102933967B (en) | Sample analysis device and sample analysis method | |
| WO2015053290A1 (en) | Chemiluminescence measurement device and method for same | |
| CN101561517B (en) | Non-contact type detector and detecting method of liquid in pipe | |
| ATE377752T1 (en) | READING APPARATUS, METHOD AND SYSTEM FOR LATERAL FLOW ASSAY TEST STRIPS | |
| JPH03154853A (en) | Chemical method and device for identifying dissimilar luminescence | |
| CN106796226B (en) | Target substance assay kit, target substance measurement system, immune chromatograph assay kit and immune chromatograph measure system | |
| CN101545864A (en) | Optical analysis reading device | |
| US20070116595A1 (en) | Assaying test strips having different capture reagents | |
| CN112362708B (en) | Preparation method of self-powered bipolar microelectrode microfluidic chip photoelectrochemical aptamer sensor | |
| CN201210144Y (en) | Optical analysis reading device | |
| CN109211867A (en) | A kind of micro-fluidic fluorescence immunoassay chip of rapid quantitative detection BNP | |
| CN105021578A (en) | Fluid fluorescence quantitative detection apparatus and fluid fluorescence quantitative detection method | |
| JPH05172815A (en) | Immunity analyzing method and analyzing device therefor | |
| CN102301238A (en) | Automatic Analyzer | |
| CN102128830A (en) | Immunochromatographic test paper strip color signal quantitative detector | |
| CN101865849B (en) | weak light detection device | |
| JP2007139658A (en) | Allergen detection method and allergen detection device | |
| CN108362642B (en) | Chemiluminescence immunoassay photometry device and method | |
| CN109917122A (en) | A kind of chemiluminescence detecting | |
| KR20150053386A (en) | Lateral flow assay type diagnostic strip for electro-chemiluminescence, Reading device thereof and Detecting method using the same | |
| CN204964518U (en) | Antibody interact's quick detection device | |
| CN205691502U (en) | A kind of magnetic microsphere fluorescence detecting system | |
| CN207148120U (en) | Chemiluminescence immune assay detects camera bellows control photo structure | |
| CN105911267A (en) | Urine analyzer light control device | |
| CN208297538U (en) | Detection device for chemiluminescence immune assay |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |