CN109913365A - A kind of aquatic products is quickly spread cultivation device and application method with probiotics - Google Patents
A kind of aquatic products is quickly spread cultivation device and application method with probiotics Download PDFInfo
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- CN109913365A CN109913365A CN201910332796.3A CN201910332796A CN109913365A CN 109913365 A CN109913365 A CN 109913365A CN 201910332796 A CN201910332796 A CN 201910332796A CN 109913365 A CN109913365 A CN 109913365A
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- filter
- air
- probiotics
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- culture apparatus
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Abstract
The invention discloses a kind of aquatic products quickly to be spread cultivation device, including air compensating system, culture apparatus, stirring system, exhaust gas filter, feed supplement and sampling system with probiotics；The air compensating system, the culture apparatus, the feed supplement and sampling system are sequentially connected by silica gel hose；The culture apparatus is equipped with gas outlet, and the gas outlet is equipped with the exhaust gas filter；The stirring system is stirred the feed liquid in the culture apparatus；The air compensating system includes the feeder for passing sequentially through silica gel hose connection, low efficient filter, high efficiency particulate air filter.Apparatus of the present invention can be used for saccharomycete used for aquiculture, lactic acid bacteria, bacillus and expand culture.
Description
Technical field
The present invention relates to probiotic's culture technical field used for aquiculture, in particular to aquatic products is quickly spread cultivation dress with probiotics
It sets and application method.
Background technique
Probiotics is that active bacteria formulation made of a kind of normal profitable strain separated out of host and microbial fermentation produce
Product mainly have in aquaculture using more extensive beneficial bacterium: bacillus, photosynthetic bacteria, lactic acid bacteria, nitrobacteria with
And saccharomycete.Probiotics is favored because of that will not can generate toxic side effect as antibiotic by numerous aquaculturists,
In aquaculture mainly have degradation organic matter, decomposition and inversion harmful substance balance water body Tiny ecosystem, inhibit pernicious bacteria breeding,
The effects of enhancing cultivated animals immunity, maintenance algae are balanced each other, " low yield trains algae, high yield cultivation " also comes therefrom, it is seen that prebiotic
Influence of the element to aquaculture is huge.
Summary of the invention
It is quickly spread cultivation device in consideration of it, the present invention provides a kind of aquatic products with probiotics, to solve probiotics in the prior art
The problems such as culture is slow, easy to pollute, and equipment input cost is big.
The technical scheme of the present invention is realized as follows:
A kind of aquatic products is quickly spread cultivation device with probiotics, which is characterized in that including air compensating system, culture apparatus, stirring system
System, exhaust gas filter, feed supplement and sampling system；The air compensating system, the culture apparatus, the feed supplement and sampling system are logical
Silica gel hose is crossed to be sequentially connected；The culture apparatus is equipped with gas outlet, and the gas outlet is equipped with the exhaust gas filter；It is described to stir
System is mixed to be stirred the feed liquid in the culture apparatus；The air compensating system includes the confession for passing sequentially through silica gel hose connection
Device of air, low efficient filter, high efficiency particulate air filter.
Further, the feeder is air pump or air compressor.
Further, filter core used in the low efficient filter is the bellows filter core or membrane type filter core of 50-100 micron pore size,
Its material is PP cotton or polytetrafluoroethylene (PTFE).
Further, filter core used in the high efficiency particulate air filter is the bellows filter core or membrane type filter core of 0.5-0.1 micron pore size,
Its material is PP cotton or polytetrafluoroethylene (PTFE).
Further, the stirring system includes magnetic stirring apparatus or propeller agitator, is made by being driven by electricity
Culture solution mixing.
Further, the culture apparatus is triangular flask or slot vial.
Further, the feed supplement and sampling system include feed supplement bottle, and the feed supplement bottle passes through silica gel hose and peristaltic pump
It is connect with culture apparatus.
Further, a kind of aquatic products is quickly spread cultivation the application method of device with probiotics, comprising the following steps: is being trained first
It supports in device and prepares culture medium, be placed into autoclave and carry out high-temperature sterilization；Then strain is accessed after culture medium is cooling；Later
The air pump for opening air compensating system, pumps air into low efficient filter, degerming is carried out using high efficiency particulate air filter, by being sterile filtered
Air import culture apparatus in carry out air-blowing culture, culture exhaust gas by exhaust gas filter discharge；Feed supplement and sampling system
Feed liquid in feed supplement bottle, which is delivered in culture apparatus by peristaltic pump through hose, carries out feed supplement；The feed liquid of culture apparatus passes through stirring
System carries out uniformly mixed.
Compared with prior art, what the present invention reached has the beneficial effect that:
(1) apparatus of the present invention can be used for saccharomycete used for aquiculture, lactic acid bacteria, bacillus and expand culture.
(2) present device input cost is lower, can in simple environment can mass propgation aquatic products use probiotics；
(3) low efficient filter and high efficiency particulate air filter of the present invention are used in combination, and the overwhelming majority that can be removed in air is thin
Bacterium guarantees going on smoothly for probiotic's culture；
(4) probiotic's culture whole process is isolated with external environment in the present invention, has prevented entering for external contaminants
It invades.
Detailed description of the invention
Fig. 1 is that aquatic products of the present invention is quickly spread cultivation the structural schematic diagram of device with probiotics；
In figure: air pump 1, low efficient filter 2, high efficiency particulate air filter 3, exhaust gas filter 4, culture apparatus 5, magnetic stirring apparatus 6,
Peristaltic pump 7, feed supplement bottle 8, air inlet 9, gas outlet 10, feed inlet 11, stirrer 12.
Specific embodiment
In order to be best understood from the technology of the present invention content, be provided below specific embodiment, and in conjunction with attached drawing to the present invention do into
The explanation of one step.
A kind of aquatic products as shown in Figure 1 is quickly spread cultivation device with probiotics, including air compensating system, culture apparatus 5, stirring system
System, exhaust gas filter 4, feed supplement and sampling system；The air compensating system, the culture apparatus 5, the feed supplement and sampling system are equal
It is sequentially connected by silica gel hose；The culture apparatus 5 is equipped with gas outlet 10, and the gas outlet 10 is equipped with the exhaust gas filter
4；The stirring system is stirred the feed liquid in the culture apparatus 5；The air compensating system is soft including passing sequentially through silica gel
Feeder, the low efficient filter 2, high efficiency particulate air filter 3 of pipe connection, high efficiency particulate air filter 3 are connected to the air inlet 9 of culture apparatus.Institute
Stating feeder is air pump 1 or air compressor.Filter core used in the low efficient filter 2 is that the bellows of 50-100 micron pore size are filtered
Core or membrane type filter core, material are PP cotton or polytetrafluoroethylene (PTFE).Filter core used in the high efficiency particulate air filter 3 is 0.5-0.1 micron openings
The bellows filter core or membrane type filter core of diameter, material are PP cotton or polytetrafluoroethylene (PTFE).The stirring system include magnetic stirring apparatus 6,
Stirrer 12 or propeller agitator mix culture solution by being driven by electricity.The culture apparatus 5 is triangular flask or narrow
Mouth vial.Feed supplement and sampling system include feed supplement bottle 8, and feed supplement bottle 8 passes through silica gel hose and peristaltic pump 7 and culture apparatus 5
Feed inlet 11 connects.
The culture apparatus 5 is with air compensating system by way of stainless steel metal pipe and silica gel plug or cotton plug and culture
Bottle is attached, and the metal tube outlet is immersed in the culture solution of culture apparatus 5, guarantees that filtrated air can be filled with culture solution
Divide mixing.Culture apparatus 5 carries out continuing stirring by magnetic agitation or propeller agitator, guarantee culture solution and air,
Feed supplement is quickly sufficiently mixed.It is controlled by the revolving speed of magnetic stirring apparatus 6 or agitating paddle to adjust mixed effect, to reach most
Big mixed effect.The setting of tail gas hose inlet is higher than culture solution liquid level, to guarantee being smoothly discharged for waste gases.The feed supplement bottle
8 and feed supplement pipe driven by peristaltic pump 7, feed liquid is pumped into culture bottle by timing constant speed.The feed supplement hose passes through stainless
The form of steel metal pipe and silica gel plug or cotton plug is attached with culture bottle and feed supplement bottle 8, and the metal tube arrival end is answered
Be immersed in 8 bottom of feed supplement bottle, to guarantee that feed supplement can be pumped all in culture solution, outlet should be immersed in culture solution, guarantee feed supplement with
Culture solution is sufficiently mixed.
Aquatic products of the present invention is quickly spread cultivation the application method of device with probiotics, comprising the following steps: first in culture bottle
Culture medium is prepared, is placed into autoclave and carries out high-temperature sterilization；Then strain is accessed after culture medium is cooling；Air pump is opened later
1, air pump 1 is entered into low efficient filter 2, carries out degerming using high efficiency particulate air filter 3, the air by aseptic filtration imports culture
Air-blowing culture is carried out in bottle；It cultivates exhaust gas to be discharged by exhaust gas filter 4,8 feed liquid of feed supplement bottle is conveyed by peristaltic pump 7 through hose
Feed supplement is carried out into culture bottle；The feed liquid of culture bottle is uniformly mixed by magnetic stirring apparatus 6.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent substitution, improvement and etc. done be should be included within the scope of the present invention.
Claims (8)
1. a kind of aquatic products is quickly spread cultivation device with probiotics, which is characterized in that including air compensating system, culture apparatus, stirring system,
Exhaust gas filter, feed supplement and sampling system；The air compensating system, the culture apparatus, the feed supplement and sampling system pass through
Silica gel hose is sequentially connected；The culture apparatus is equipped with gas outlet, and the gas outlet is equipped with the exhaust gas filter；The stirring
System is stirred the feed liquid in the culture apparatus；The air compensating system includes the gas supply for passing sequentially through silica gel hose connection
Device, low efficient filter, high efficiency particulate air filter.
2. a kind of aquatic products according to claim 2 is quickly spread cultivation device with probiotics, the feeder is air pump or sky
Air compressor.
3. a kind of aquatic products according to claim 1 or 2 is quickly spread cultivation device with probiotics, it is characterised in that: the thick effect
Filter core used in filter is the bellows filter core or membrane type filter core of 50-100 micron pore size, and material is PP cotton or polytetrafluoroethylene (PTFE).
4. a kind of aquatic products according to claim 3 is quickly spread cultivation device with probiotics, it is characterised in that: the high efficiency filter
Filter core used in device is the bellows filter core or membrane type filter core of 0.5-0.1 micron pore size, and material is PP cotton or polytetrafluoroethylene (PTFE).
5. a kind of aquatic products according to claim 1,2 or 4 is quickly spread cultivation device with probiotics, it is characterised in that: described to stir
The system of mixing includes magnetic stirring apparatus or propeller agitator.
6. a kind of aquatic products according to claim 5 is quickly spread cultivation device with probiotics, it is characterised in that: the culture apparatus
For triangular flask or slot vial.
7. a kind of aquatic products according to claim 1 or 6 is quickly spread cultivation device with probiotics, it is characterised in that: the feed supplement
And sampling system includes feed supplement bottle, the feed supplement bottle is connect by silica gel hose and peristaltic pump with culture apparatus.
The application method of device 8. a kind of aquatic products according to claim 1 is quickly spread cultivation with probiotics, which is characterized in that packet
It includes following steps: preparing culture medium in culture apparatus first, be placed into autoclave and carry out high-temperature sterilization；Then to culture medium
Strain is accessed after cooling；Later open air compensating system air pump, pump air into low efficient filter, using high efficiency particulate air filter into
Row degerming, the air by aseptic filtration import and carry out air-blowing culture in culture apparatus, and culture exhaust gas is arranged by exhaust gas filter
Out；Feed liquid in the feed supplement bottle of feed supplement and sampling system, which is delivered in culture apparatus by peristaltic pump through hose, carries out feed supplement；Training
The feed liquid of feeding device is carried out uniformly mixed by stirring system.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910332796.3A CN109913365A (en) | 2019-04-24 | 2019-04-24 | A kind of aquatic products is quickly spread cultivation device and application method with probiotics |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910332796.3A CN109913365A (en) | 2019-04-24 | 2019-04-24 | A kind of aquatic products is quickly spread cultivation device and application method with probiotics |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109913365A true CN109913365A (en) | 2019-06-21 |
Family
ID=66978330
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910332796.3A Withdrawn CN109913365A (en) | 2019-04-24 | 2019-04-24 | A kind of aquatic products is quickly spread cultivation device and application method with probiotics |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109913365A (en) |
-
2019
- 2019-04-24 CN CN201910332796.3A patent/CN109913365A/en not_active Withdrawn
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| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20190621 |
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| WW01 | Invention patent application withdrawn after publication |