CN109913344A - A kind of method that stage extraction prepares health liquor animal former wine - Google Patents
A kind of method that stage extraction prepares health liquor animal former wine Download PDFInfo
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- CN109913344A CN109913344A CN201910345504.XA CN201910345504A CN109913344A CN 109913344 A CN109913344 A CN 109913344A CN 201910345504 A CN201910345504 A CN 201910345504A CN 109913344 A CN109913344 A CN 109913344A
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- extraction
- ethyl alcohol
- pancreatin
- papain
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Abstract
The invention discloses a kind of methods that stage extraction prepares health liquor animal former wine, mainly include two stages；The height ethyl alcohol extraction stage mainly includes the following steps: (1) ingredient inspection；(2) it cuts off；(3) it cleans；(4) it processes；(5) it weighs；(6) alcohol extracting one is impregnated；(7) it filters, obtains height ethanol extraction filtrates and raw material filter residue；The low ethyl alcohol extraction stage mainly includes the following steps: that (8) are broken；(9) pancreatin hydrolysis；(10) papain hydrolysis；(11) alcohol extracting two is impregnated；(12) it filters；(13) it mixes.After the present invention, the health liquor of same standard is prepared, extraction time was shortened to 10 months by original 24 months；Making the cost of health liquor per ton reduces by 50%；The ingredient of extract is identical as the ingredient that former extracting method is extracted, and not will increase harmful components, nor affects on the mouthfeel and effect of health liquor obtained.
Description
Technical field
The present invention relates to a kind of methods that stage extraction prepares health liquor animal former wine, belong to food processing technology field.
Background technique
Animals and plants are impregnated as solvent using wine and produce health liquor in the existing very long history of China, and preparation method predominantly passes through leaching
Bubble is dissolved in the effective component in raw material in ethanol solution.Traditional health care wine generally using terrestrial and freshwater animals and plants as
Then there are the tradition using marine animal as raw material in raw material, coastal area.But with the increase of health liquor yield sales volume, as
The animals and plants that raw material uses are gradually insufficient, especially the marine animal of coastal area, and tracing it to its cause is that certain class animals and plants needs
Will from natural sea area catch obtain, stock number it is limited and can not propagate artificially or propagate artificially material effective component drop
It is low.The essence of health liquor is mainly the effective component passed through in ethyl alcohol soak extraction raw material, therefore in order to solve animal material money
The problem of source deficiency, can seek and how to improve extracting method, improve the recovery rate and utilization rate of effective component.
Summary of the invention
Technical problem to be solved by the invention is to provide a kind of methods that stage extraction prepares health liquor animal former wine, use
In the effective component extraction rate and utilization rate that improve animal material in health liquor.
Technical scheme is as follows: a kind of method that stage extraction prepares health liquor animal former wine, and main includes height
It spends ethyl alcohol and extracts stage and low ethyl alcohol extraction stage；
The height ethyl alcohol extraction stage mainly includes the following steps: (1) ingredient inspection；(2) it cuts off；(3) it cleans；(4) it processes；(5)
It weighs；(6) alcohol extracting one is impregnated；(7) it filters, obtains height ethanol extraction filtrates and raw material filter residue；
The low ethyl alcohol extraction stage mainly includes the following steps:
(8) it is crushed: raw material filter residue being crushed, filter residue broken material is obtained；
(9) pancreatin hydrolysis: according to filter residue broken material: pancreatin is added, in pH7.5 condition in pancreatin=100:0.25 parts by weight ratio
Lower hydrolysis 5 hours, is then heated to 90 DEG C, keeps the temperature 30 minutes, inactivates pancreatin, obtains pancreatin and extracts material；
(10) papain hydrolysis: extract and expect according to pancreatin: wood is added in papain=100:0.15 parts by weight ratio
Melon protease hydrolyzes 3 hours under the conditions of pH7.0, is then heated to 90 DEG C, keeps the temperature 30 minutes, and papain of putting out a fire obtains
Papain extracts material；
(11) it impregnates alcohol extracting two: papain is extracted into material with low ethyl alcohol (30%vol) by 1:4 mixing soak extraction 3
Month, obtain low ethanol extract；
(12) it filters: obtaining low ethanol extraction filtrates；
(13) it mixes: height ethanol extraction filtrates and low ethanol extraction filtrates is mixed by different proportion and prepare health liquor to obtain the final product
Required animal former wine.
The height ethyl alcohol refers to 53 degree of white wine；Low ethyl alcohol refers to 30 degree of white wine.
The animal material is sea snake.
When effective component in conventional height ethyl alcohol soak extraction animal material, height ethyl alcohol easily causes material protein table
Face denaturation, after making the protein denaturation of protein coagulating, especially cell membrane surface and space between cells, solidification, intraor extracellular
Can molten, the water-soluble effective component of alcohol be sealed and be not easy to dissolve out, this part effective component cannot be extracted to, and lead to recovery rate
It is low.If directly extracted using low ethyl alcohol, extraction rate is slow, and low ethyl alcohol water content is high, and it is rotten to easily lead to raw material.Sea snake leaching
The result for steeping traditional extraction process and extracting method of the present invention is as shown in the table:
| Sample | Steroid saponin | Lipid (thin layer) | Protein content |
| 38oWhite wine | It is negative | It is negative | It is not detected |
| Sea snake impregnates 1 year (conventional immersion) | It is positive | It is consistent with reference substance | 0.22% |
| Sea snake impregnates 2 years (conventional immersion) | It is positive | It is consistent with reference substance | 0.32% |
| Sea snake powder the 3rd time (the method for the invention) | It is positive | It is consistent with reference substance | 0.36% |
| Sea snake powder the 2nd time (the method for the invention) | It is positive | It is consistent with reference substance | 0.49% |
| Sea snake powder the 1st time (the method for the invention) | It is positive | It is consistent with reference substance | 0.77% |
Extraction is divided into two stages by the present invention, and the first stage is extracted the Alcohol soluble composition in raw material with height ethyl alcohol
Afterwards, raw material is crushed, then using pancreatin, the double enzymes enzymatic hydrolysis of papain as animal material cell and space between cells skeleton structure
Rupture means keep raw material and the contact of ethyl alcohol and water more abundant, further extract water soluble ingredient and remaining alcohol-soluble
Ingredient.After the present invention, the health liquor of same standard is prepared, extraction time was shortened to 10 months by original 24 months；Make every
The cost of ton health liquor reduces by 50%；The ingredient of extract is identical as the ingredient that former extracting method is extracted, not will increase it is harmful at
Point, nor affect on the mouthfeel and effect of health liquor obtained.
Specific embodiment
The present invention is embodied using the eastern garden of Beihai Hepu County Dong Yuanjia brewery family's wine as health liquor produced, with
The animal material that the maximum sea snake of dosage changes as evaluation recovery rate in eastern garden man wine, with " Guangxi Chinese herbal medicine standard " second
The method of inspection of middle sea snake is test basis, to the east of the garden obtained animal former wine of family's wine traditional ethanol extracting method as control ginseng
Examine standard.Used pancreatin is Pangbo Bioengineering Co Ltd, Nanning's production, and 4000u/g, papain is that Nanning is huge
Rich bioengineering Co., Ltd production, 3,500,000 units/grams.
Embodiment one
Height ethyl alcohol extracts the stage: (1) choosing 2 kg sea snakes and examine；(2) it is cut to a cun section；(3) it cleans；(4) it processes；(5) claim
Amount；(6) raw material is put into 53 degree of white wine of 10kg and is impregnated 180 days；(7) it filters, obtains 8kg height ethanol extraction filtrates and 4kg
Raw material filter residue；
Low ethyl alcohol extracts the stage: (8) are broken: raw material filter residue being crushed, filter residue broken material is obtained；(9) pancreatin hydrolysis: according to filter
Slag broken material: pancreatin=100:0.25 parts by weight ratio is added 0.01g pancreatin, adds water to 10kg and hydrolyze 5 under the conditions of pH7.5
Hour, 90 DEG C are then heated to, keeps the temperature 30 minutes, inactivates pancreatin, 10kg pancreatin is obtained and extracts material；(10) papain water
Solution: extract and expect according to pancreatin: 0.015g papain is added in papain=100:0.15 parts by weight ratio,
It is hydrolyzed 3 hours under the conditions of pH7.0, is then heated to 90 DEG C, keep the temperature 30 minutes, inactivated papain, obtain 10kg pawpaw egg
White enzyme extracts material；(11) impregnate alcohol extracting two: papain being extracted into material, 40kg(30 degree is added) soak extraction 3 in ethyl alcohol
Month, obtain 50kg minuent ethanol extract；(12) it filters: obtaining 48.5kg minuent ethanol extraction filtrates；
(13) it mixes: height ethanol extraction filtrates and low ethanol extraction filtrates is mixed by different proportion and prepare health liquor to obtain the final product
Required animal former wine.
Embodiment two
Height ethyl alcohol extracts the stage: (1) choosing 3 kg sea snakes and examine；(2) it is cut to a cun section；(3) it cleans；(4) it processes；(5) claim
Amount；(6) raw material is put into 53 degree of white wine of 15kg and is impregnated 180 days；(7) filter, obtain 12kg height ethanol extraction filtrates and
6kg raw material filter residue；
Low ethyl alcohol extracts the stage: (8) are broken: raw material filter residue being crushed, filter residue broken material is obtained；(9) pancreatin hydrolysis: according to filter
Slag broken material: pancreatin=100:0.25 parts by weight ratio is added 0.015g pancreatin, adds water to 15kg and hydrolyze under the conditions of pH7.5
5 hours, 90 DEG C are then heated to, keeps the temperature 30 minutes, inactivates pancreatin, 15kg pancreatin is obtained and extracts material；(10) papain water
Solution: extract and expect according to pancreatin: 0.023g papain is added in papain=100:0.15 parts by weight ratio,
It is hydrolyzed 3 hours under the conditions of pH7.0, is then heated to 90 DEG C, keep the temperature 30 minutes, papain of putting out a fire obtains 15kg pawpaw egg
White enzyme extracts material；(11) impregnate alcohol extracting two: papain being extracted into material, 60kg(30 degree is added) in ethyl alcohol, soak extraction 6
Month, obtain 75kg minuent ethanol extract；(12) it filters: obtaining 72.8kg minuent ethanol extraction filtrates；
(13) it mixes: height ethanol extraction filtrates and low ethanol extraction filtrates is mixed by different proportion and prepare health liquor to obtain the final product
Required animal former wine.
Claims (1)
1. a kind of method that stage extraction prepares health liquor animal former wine mainly includes that height ethyl alcohol extracts stage and low ethyl alcohol
The extraction stage；
The height ethyl alcohol extraction stage mainly includes the following steps: (1) ingredient inspection；(2) it cuts off；(3) it cleans；(4) it processes；(5)
It weighs；(6) alcohol extracting one is impregnated；(7) it filters, obtains height ethanol extraction filtrates and raw material filter residue；
The low ethyl alcohol extraction stage mainly includes the following steps: that (8) are broken: raw material filter residue being crushed, filter residue broken material is obtained；
(9) pancreatin hydrolysis: according to filter residue broken material: pancreatin=100:0.25 parts by weight ratio is added pancreatin, is lauched in pH7.5 condition
Solution 5 hours is then heated to 90 DEG C, keeps the temperature 30 minutes, inactivates pancreatin, obtains pancreatin and extracts material；(10) papain hydrolysis:
Extract and expect according to pancreatin: papain=100:0.15 parts by weight ratio is added papain, is lauched in pH7.0 condition
Solution 3 hours is then heated to 90 DEG C, keeps the temperature 30 minutes, inactivates papain, obtains papain and extracts material；(11) it soaks
It steeps alcohol extracting two: papain being extracted into material with low ethyl alcohol (30%vol) by 1:4 mixing soak extraction 3 months, obtains minuent
Ethanol extract；(12) it filters: obtaining low ethanol extraction filtrates；
(13) it mixes: height ethanol extraction filtrates and low ethanol extraction filtrates is mixed by different proportion and prepare health liquor to obtain the final product
Required animal former wine；
The height ethyl alcohol refers to 53 degree of white wine；Low ethyl alcohol refers to 30 degree of white wine；The raw material is sea snake.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1191116A (en) * | 1997-02-19 | 1998-08-26 | 方晓阳 | Oral snake liquid |
| CN101926826A (en) * | 2010-09-11 | 2010-12-29 | 海南壹号药业有限公司 | Method for extracting active ingredients from sea horse |
| CN103173512A (en) * | 2013-03-07 | 2013-06-26 | 安徽丰润生物技术有限公司 | Process for extracting Penis et Testis Canis polypeptide through enzymatic method, and obtained Penis et Testis Canis polypeptide extract |
| CN104435173A (en) * | 2014-12-16 | 2015-03-25 | 安徽高山药业有限公司 | Health medicinal liquor and production method thereof |
-
2019
- 2019-04-26 CN CN201910345504.XA patent/CN109913344A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1191116A (en) * | 1997-02-19 | 1998-08-26 | 方晓阳 | Oral snake liquid |
| CN101926826A (en) * | 2010-09-11 | 2010-12-29 | 海南壹号药业有限公司 | Method for extracting active ingredients from sea horse |
| CN103173512A (en) * | 2013-03-07 | 2013-06-26 | 安徽丰润生物技术有限公司 | Process for extracting Penis et Testis Canis polypeptide through enzymatic method, and obtained Penis et Testis Canis polypeptide extract |
| CN104435173A (en) * | 2014-12-16 | 2015-03-25 | 安徽高山药业有限公司 | Health medicinal liquor and production method thereof |
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