CN109913312A - A kind of emulsion breakdown method that aqueous enzymatic extraction Rice oil is formed - Google Patents
A kind of emulsion breakdown method that aqueous enzymatic extraction Rice oil is formed Download PDFInfo
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- 235000009566 rice Nutrition 0.000 title claims abstract description 136
- 239000000839 emulsion Substances 0.000 title claims abstract description 80
- 238000000034 method Methods 0.000 title claims abstract description 37
- 230000015556 catabolic process Effects 0.000 title claims abstract description 27
- 230000002255 enzymatic effect Effects 0.000 title claims abstract description 27
- 238000000605 extraction Methods 0.000 title claims abstract description 27
- 240000007594 Oryza sativa Species 0.000 title description 2
- 241000209094 Oryza Species 0.000 claims abstract description 135
- 239000003921 oil Substances 0.000 claims abstract description 58
- 239000007788 liquid Substances 0.000 claims abstract description 26
- 238000012545 processing Methods 0.000 claims abstract description 26
- 239000004519 grease Substances 0.000 claims abstract description 17
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 11
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- 206010065954 Stubbornness Diseases 0.000 claims abstract description 5
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- 102000004190 Enzymes Human genes 0.000 claims description 33
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- 239000003513 alkali Substances 0.000 claims description 20
- 230000009849 deactivation Effects 0.000 claims description 16
- 239000000843 powder Substances 0.000 claims description 16
- 101710130006 Beta-glucanase Proteins 0.000 claims description 10
- 108091005804 Peptidases Proteins 0.000 claims description 10
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- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 10
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- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 7
- 238000005265 energy consumption Methods 0.000 abstract description 4
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- 108010059892 Cellulase Proteins 0.000 abstract 1
- 229940106157 cellulase Drugs 0.000 abstract 1
- 235000019198 oils Nutrition 0.000 description 44
- 239000008157 edible vegetable oil Substances 0.000 description 8
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 3
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 3
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Landscapes
- Fats And Perfumes (AREA)
- Edible Oils And Fats (AREA)
Abstract
The invention discloses a kind of emulsion breakdown method that aqueous enzymatic extraction Rice oil is formed, emulsion layer, hydrolysis liquid layer, residual layer is can be obtained in the rice bran zymolyte obtained after cellulase treatment after centrifugation.Emulsion layer is taken, pH is adjusted, after centrifugation, free oil and obstinate emulsion is obtained, adds a certain amount of calcium chloride solution to obstinate emulsion, after centrifugation, obtain upper layer grease, the sum of the resulting free oil that is demulsified twice is total Rice bran crude oil.Adjusting pH method is to achieve the purpose that the structure and charging property that change interfacial film, to effectively be demulsified by changing emulsion system pH environment.Still the double electrical layers of protein are destroyed, generation aggregation between oil droplet is promoted to cause to be demulsified by the way that calcium chloride solution is added with the presence of part stubbornness emulsion after first demulsification centrifugation.Demulsification efficiency can be effectively improved by carrying out demulsification processing to the emulsion that aqueous enzymatic extraction Rice oil generates using breaking method provided by the invention, oryzanol content also significantly improves in gained Rice bran crude oil simultaneously, has low energy consumption, pollution-free, green and healthy, simple operation and other advantages.
Description
Technical field
The present invention relates to a kind of processing methods of aqueous enzymatic extraction Rice oil, and especially one kind is in aqueous enzymatic extraction Rice oil
In the process, emulsion breakdown is carried out by combining using adjusting pH method with electrostatic screen method, so that the method for improving demulsification efficiency, belongs to
In field of food industry.
Background technique
China is maximum eating rice production state in the world, and rice bran is as the important by-products during Rice producing, mainly
It is made of exocarp, middle pericarp, cross-linked layer, kind skin and aleurone.Rice bran is as rice oil extract raw material, and wherein oil quality divides
Number is up to 12-22%.Rice oil is a kind of novel edible vegetable oil important, with high nutritive value, wherein the oleic acid contained,
Linoleic acid plus linolenic acid mass fraction is up to 80% or more, and oleic acid and linoleic ratio meet international organization and pushed away close to 1: 1
The oleic acid and linoleic acid optimal proportion recommended.Also contain more oryzanol, tocotrienols, squalene and plant in Rice oil simultaneously
The important biomolecules active material such as object sterol, nutritive value is far more than traditional edible vegetable oil such as peanut oil, soybean oil.At present
Have the effects that the cholesterol in removing blood there are many research confirmation Rice oil, reduces blood lipid, promotes growth in humans's development, because
This is described as nutrition oil by nutritionist.
Currently, common Rice oil extracting method includes organic solvent extraction, physical squeezing method and aqueous enzymatic method.Aqueous enzymatic method
It is by oil-containing raw material by broken in various degree, is medium by adding specific biological enzyme, enzyme under conditions of most suitable using water
Oil and non-oil component are divided the difference of oil and water affinity and the different of grease density using non-oil component by solution certain time
From.More other two methods, aqueous enzymatic extraction is with oil quality is good, low energy consumption, equipment is simple, excellent without chemical contamination etc.
Point is a kind of to propose oily new technology with broad prospect of application.
During aqueous enzymatic extraction Rice oil, oil and albumen are digested simultaneously, since albumen has good emulsibility,
It can be adsorbed between grease rapidly, form interfacial film and be wrapped in outside oil droplet, to form stable oil-in-water emulsified system.Cause
This first isolated free oil is very limited, and most oil is all present in emulsion layer, although mild enzymatic hydrolysis item
Part is conducive to save the active skull cap components in grease, but generates a large amount of emulsion while mentioning oil and can significantly reduce grease
Oil yield, thus limit aqueous enzymatic method mention oil development.
Summary of the invention
The purpose of the present invention is to above-mentioned defect in the prior art, a kind of aqueous enzymatic extraction Rice oil is provided
Breaking method is demulsified to the emulsion of generation by being combined using adjusting pH method with electrostatic screen method, further increases brokenly
Newborn rate, lower production costs meet the environmental requirement of " safety, green ".
To achieve the above object, the present invention adopts the following technical scheme:
A kind of emulsion breakdown method that aqueous enzymatic extraction Rice oil is formed, operating procedure are as follows:
(1) rice bran sample treatment: carry out the rice bran Jing Guo expanding treatment to crushed 40 meshes obtaining rice bran powder;
(2) it is enzymatically treated: deionized water will be added in rice bran powder that step (1) obtains, be tuned by certain solid-liquid ratio mixed
Liquid is closed, mixed liquor is warming up to 90 DEG C of enzyme deactivation 15min, is cooled to room temperature later.Rice bran mixing is adjusted with the HCL solution of 2mol/L
Liquid pH to 5.0 adds a certain amount of 1,4 beta-glucanase and plant extract enzyme, is digested to obtain first rice bran zymolyte.With
The NaOH solution of 2mol/L adjusts first rice bran zymolyte pH to 9.0, the alkali carries certain time at 60 DEG C.2mol/L is used later
NaOH solution continue adjust pH to 9.0, add a certain amount of Acalase2.4L alkali protease, carry out under certain condition
Enzymatic hydrolysis, obtains final rice bran zymolyte.Obtained rice bran zymolyte is placed in enzyme deactivation 10min in 90 DEG C of water-baths, later one
It is centrifuged under fixed condition, obtains emulsion layer, hydrolysis liquid layer, residual layer;
(3) emulsion breakdown: the emulsion that step (2) is obtained takes out, and stands 12h at 4 DEG C, carries out at demulsification to it
Reason, is centrifuged under certain condition, upper layer grease is taken out, and lower layer's stubbornness emulsion takes out spare.The stubbornness that will be obtained after centrifugation
Emulsion carries out demulsification processing again, is centrifuged under certain condition, take out upper layer free oil, be demulsified twice resulting free oil it
As total Rice bran crude oil.
Further, the method for the emulsion breakdown that a kind of above-mentioned aqueous enzymatic extraction Rice oil is formed, the step (2)
Middle solid-liquid ratio, that is, rice bran weight: deionized water weight 1: (5~8).
Further, the method for the emulsion breakdown that a kind of above-mentioned aqueous enzymatic extraction Rice oil is formed, the step (2)
The amount of middle 1,4 beta-glucanase and plant extract enzyme is 1%~5%, and the two ratio is 1: (1~3), enzymatic hydrolysis condition are 50 DEG C, 1h~
3h。
Further, the method for the emulsion breakdown that a kind of above-mentioned aqueous enzymatic extraction Rice oil is formed, the step (2)
The middle alkali carries time is 5min~60min.
Further, the method for the emulsion breakdown that a kind of above-mentioned aqueous enzymatic extraction Rice oil is formed, the step (2)
The amount of middle Acalase2.4L alkali protease is 1%~5%, and enzymatic hydrolysis condition is 60 DEG C, 2h~3h.
Further, the method for the emulsion breakdown that a kind of above-mentioned aqueous enzymatic extraction Rice oil is formed, the step (2)
Centrifugal condition with step (3) is 6000r/min~10000r/min, 10min~30min.
Further, the method for the emulsion breakdown that a kind of above-mentioned aqueous enzymatic extraction Rice oil is formed, the step (3)
In twice demulsification processing be respectively adjust emulsion pH to 3.5~5.5, at 40 DEG C~80 DEG C, water bath processing 1h~3h;Addition
0.03mol/L~0.1mol/L calcium chloride solution, at 60 DEG C~80 DEG C, water bath processing 10min~90min.
The beneficial effects are mainly reflected as follows carry out brokenly to the emulsion generated during aqueous enzymatic extraction Rice oil
Cream processing, adjusting pH method is to achieve the purpose that the structure and charging property that change interfacial film by changing emulsion system pH environment,
To effectively be demulsified.Calcium chloride solution is added in obstinate emulsion, stable protein double electrical layers will be destroyed, promote oil
Aggregation occurs between drop to be caused to be demulsified.Two kinds of breaking methods joint can effectively improve demulsification efficiency, have low energy consumption, pollution-free, green
Color health, simple operation and other advantages.
Detailed description of the invention
Fig. 1 is the process flow for the emulsion breakdown method that a kind of aqueous enzymatic extraction Rice oil of the present invention is formed
Figure.
Specific embodiment
The present invention is further explained and described combined with specific embodiments below, but protection scope of the present invention is simultaneously
It is not limited only to this.
Comparative example 1
(1) rice bran sample treatment: carry out the rice bran Jing Guo expanding treatment to crushed 40 meshes obtaining rice bran powder;
(2) be enzymatically treated: deionized water will be added in rice bran powder that step (1) obtains, by 1: 6 (rice bran weight: go from
Sub- water weight) solid-liquid ratio is tuned into mixed liquor, and mixed liquor is warming up to 90 DEG C of enzyme deactivation 15min, is cooled to room temperature later.Use 2mol/L
HCL solution adjust rice bran pH of mixed to 5.0, add the 1,4 beta-glucanase and plant extract enzyme of rice bran weight 3%, the two ratio
Example is 1: 2, at 50 DEG C, digests 2h, obtains first rice bran zymolyte.First rice bran enzymatic hydrolysis is adjusted with the NaOH solution of 2mol/L
Object pH to 9.0, the alkali carries 10min at 60 DEG C.Continued to adjust pH to 9.0 with the NaOH solution of 2mol/L later, adds rice bran weight
The Acalase2.4L alkali protease of amount 3% digests 2h, obtains final rice bran zymolyte at 60 DEG C.The rice bran that will be obtained
Zymolyte is placed in enzyme deactivation 10min in 90 DEG C of water-baths, is centrifuged 20min under the revolving speed of 8500r/min later, obtains emulsion
Layer, hydrolysis liquid layer, residual layer;
(3) emulsion breakdown: the emulsion that step (2) is obtained takes out, and 12h is stood at 4 DEG C, adjust emulsion pH to
4.5, at 50 DEG C, water bath processing 3h.It is centrifuged 20min under the revolving speed of 8500r/min, upper layer grease is taken out, calculates demulsification
Rate and gained edible vegetable oil oryzanol content.
Comparative example 2
(1) rice bran sample treatment: carry out the rice bran Jing Guo expanding treatment to crushed 40 meshes obtaining rice bran powder;
(2) be enzymatically treated: deionized water will be added in rice bran powder that step (1) obtains, by 1: 6 (rice bran weight: go from
Sub- water weight) solid-liquid ratio is tuned into mixed liquor, and mixed liquor is warming up to 90 DEG C of enzyme deactivation 15min, is cooled to room temperature later.Use 2mol/
The HCL solution of L adjusts rice bran pH of mixed to 5.0, adds the 1,4 beta-glucanase and plant extract enzyme of rice bran weight 3%, the two
Ratio is 1: 2, at 50 DEG C, digests 2h, obtains first rice bran zymolyte.First rice bran enzyme is adjusted with the NaOH solution of 2mol/L
Solve object pH to 9.0, the alkali carries 10min at 60 DEG C.Continued to adjust pH to 9.0 with the NaOH solution of 2mol/L later, adds rice bran
The Acalase2.4L alkali protease of weight 3% digests 2h, obtains final rice bran zymolyte at 60 DEG C.The rice that will be obtained
Chaff zymolyte is placed in enzyme deactivation 10min in 90 DEG C of water-baths, is centrifuged 20min under the revolving speed of 8500r/min later, obtains emulsion
Layer, hydrolysis liquid layer, residual layer;
(3) emulsion breakdown: the emulsion that step (2) is obtained takes out, and stands 12h at 4 DEG C, adds in emulsion
0.04mol/L calcium chloride solution, at 70 DEG C, water bath processing 1h.It is centrifuged 20min under the revolving speed of 8500r/min, upper layer grease is taken
Out, demulsification efficiency and gained edible vegetable oil oryzanol content are calculated.
Comparative example 3
(1) rice bran sample treatment: carry out the rice bran Jing Guo expanding treatment to crushed 40 meshes obtaining rice bran powder;
(2) be enzymatically treated: deionized water will be added in rice bran powder that step (1) obtains, by 1: 6 (rice bran weight: go from
Sub- water weight) solid-liquid ratio is tuned into mixed liquor, and mixed liquor is warming up to 90 DEG C of enzyme deactivation 15min, is cooled to room temperature later.Use 2mol/
The HCL solution of L adjusts rice bran pH of mixed to 5.0, adds the 1,4 beta-glucanase and plant extract enzyme of rice bran weight 3%, the two
Ratio is 1: 2, at 50 DEG C, digests 2h, obtains first rice bran zymolyte.First rice bran enzyme is adjusted with the NaOH solution of 2mol/L
Solve object pH to 9.0, the alkali carries 10min at 60 DEG C.Continued to adjust pH to 9.0 with the NaOH solution of 2mol/L later, adds rice bran
The Acalase2.4L alkali protease of weight 3% digests 2h, obtains final rice bran zymolyte at 60 DEG C.The rice that will be obtained
Chaff zymolyte is placed in enzyme deactivation 10min in 90 DEG C of water-baths, is centrifuged 20min under the revolving speed of 8500r/min later, obtains emulsion
Layer, hydrolysis liquid layer, residual layer;
(3) emulsion breakdown: the emulsion that step (2) is obtained takes out, and stands 12h at 4 DEG C, adds in emulsion
0.04mol/L calcium chloride solution, at 70 DEG C, water bath processing 1h.It is centrifuged 20min under the revolving speed of 8500r/min, upper layer grease is taken
Out, lower layer's stubbornness emulsion takes out spare.The obstinate emulsion obtained after centrifugation is subjected to demulsification processing again, adjusts emulsion
PH to 4.5, at 50 DEG C, water bath processing 3h.It is centrifuged 20min under the revolving speed of 8500r/min, upper layer grease is taken out, twice
The sum of the resulting free oil that is demulsified is total Rice bran crude oil, calculates demulsification efficiency and gained edible vegetable oil oryzanol content.
Embodiment 1
(1) rice bran sample treatment: carry out the rice bran Jing Guo expanding treatment to crushed 40 meshes obtaining rice bran powder;
(2) be enzymatically treated: deionized water will be added in rice bran powder that step (1) obtains, by 1: 6 (rice bran weight: go from
Sub- water weight) solid-liquid ratio is tuned into mixed liquor, and mixed liquor is warming up to 90 DEG C of enzyme deactivation 15min, is cooled to room temperature later.Use 2mol/
The HCL solution of L adjusts rice bran pH of mixed to 5.0, adds the 1,4 beta-glucanase and plant extract enzyme of rice bran weight 3%, the two
Ratio is 1: 2, at 50 DEG C, digests 2h, obtains first rice bran zymolyte.First rice bran enzyme is adjusted with the NaOH solution of 2mol/L
Solve object pH to 9.0, the alkali carries 10min at 60 DEG C.Continued to adjust pH to 9.0 with the NaOH solution of 2mol/L later, adds rice bran
The Acalase2.4L alkali protease of weight 3% digests 2h, obtains final rice bran zymolyte at 60 DEG C.The rice that will be obtained
Chaff zymolyte is placed in enzyme deactivation 10min in 90 DEG C of water-baths, is centrifuged 20min under the revolving speed of 8500r/min later, obtains emulsion
Layer, hydrolysis liquid layer, residual layer;
(3) emulsion breakdown: the emulsion that step (2) is obtained takes out, and 12h is stood at 4 DEG C, adjust emulsion pH to
4.5, at 50 DEG C, water bath processing 3h.It is centrifuged 20min under the revolving speed of 8500r/min, upper layer grease is taken out, lower layer is obstinate
Emulsion takes out spare.The obstinate emulsion obtained after centrifugation is subjected to demulsification processing again, addition 0.04mol/L calcium chloride is molten
Liquid, at 70 DEG C, water bath processing 1h.It is centrifuged 20min under the revolving speed of 8500r/min, upper layer grease is taken out, twice demulsification gained
The sum of free oil be total Rice bran crude oil, calculate demulsification efficiency and gained edible vegetable oil oryzanol content.
Embodiment 2
(1) rice bran sample treatment: carry out the rice bran Jing Guo expanding treatment to crushed 40 meshes obtaining rice bran powder;
(2) be enzymatically treated: deionized water will be added in rice bran powder that step (1) obtains, by 1: 8 (rice bran weight: go from
Sub- water weight) solid-liquid ratio is tuned into mixed liquor, and mixed liquor is warming up to 90 DEG C of enzyme deactivation 15min, is cooled to room temperature later.Use 2mol/
The HCL solution of L adjusts rice bran pH of mixed to 5.0, adds the 1,4 beta-glucanase and plant extract enzyme of rice bran weight 4%, the two
Ratio is 1: 3, at 50 DEG C, digests 3h, obtains first rice bran zymolyte.First rice bran enzyme is adjusted with the NaOH solution of 2mol/L
Solve object pH to 9.0, the alkali carries 30min at 60 DEG C.Continued to adjust pH to 9.0 with the NaOH solution of 2mol/L later, adds rice bran
The Acalase2.4L alkali protease of weight 5% digests 3h, obtains final rice bran zymolyte at 60 DEG C.The rice that will be obtained
Chaff zymolyte is placed in enzyme deactivation 10min in 90 DEG C of water-baths, is centrifuged 30min under the revolving speed of 6000r/min later, obtains emulsion
Layer, hydrolysis liquid layer, residual layer;
(3) emulsion breakdown: the emulsion that step (2) is obtained takes out, and 12h is stood at 4 DEG C, adjust emulsion pH to
3.5, at 60 DEG C, water bath processing 2h.It is centrifuged 30min under the revolving speed of 6000r/min, upper layer grease is taken out, lower layer is obstinate
Emulsion takes out spare.The obstinate emulsion obtained after centrifugation is subjected to demulsification processing again, addition 0.1mol/L calcium chloride is molten
Liquid, at 80 DEG C, water bath processing 30min.It is centrifuged 30min under the revolving speed of 6000r/min, upper layer grease is taken out, is demulsified twice
The sum of resulting free oil is total Rice bran crude oil, calculates demulsification efficiency and gained edible vegetable oil oryzanol content.
Embodiment 3
(1) rice bran sample treatment: carry out the rice bran Jing Guo expanding treatment to crushed 40 meshes obtaining rice bran powder;
(2) be enzymatically treated: deionized water will be added in rice bran powder that step (1) obtains, by 1: 5 (rice bran weight: go from
Sub- water weight) solid-liquid ratio is tuned into mixed liquor, and mixed liquor is warming up to 90 DEG C of enzyme deactivation 15min, is cooled to room temperature later.Use 2mol/
The HCL solution of L adjusts rice bran pH of mixed to 5.0, adds the 1,4 beta-glucanase and plant extract enzyme of rice bran weight 2%, the two
Ratio is 1: 1, at 50 DEG C, digests 1h, obtains first rice bran zymolyte.First rice bran enzyme is adjusted with the NaOH solution of 2mol/L
Solve object pH to 9.0, the alkali carries 60min at 60 DEG C.Continued to adjust pH to 9.0 with the NaOH solution of 2mol/L later, adds rice bran
The Acalase2.4L alkali protease of weight 2% digests 2.5h, obtains final rice bran zymolyte at 60 DEG C.By what is obtained
Rice bran zymolyte is placed in enzyme deactivation 10min in 90 DEG C of water-baths, is centrifuged 10min under the revolving speed of 10000r/min later, obtains cream
Shape liquid layer, hydrolysis liquid layer, residual layer;
(3) emulsion breakdown: the emulsion that step (2) is obtained takes out, and 12h is stood at 4 DEG C, adjust emulsion pH to
5.5, at 80 DEG C, water bath processing 1h.It is centrifuged 10min under the revolving speed of 10000r/min, upper layer grease is taken out, lower layer is obstinate
Emulsion takes out spare.The obstinate emulsion obtained after centrifugation is subjected to demulsification processing again, addition 0.08mol/L calcium chloride is molten
Liquid, at 60 DEG C, water bath processing 90min.It is centrifuged 10min under the revolving speed of 10000r/min, upper layer grease is taken out, is demulsified twice
The sum of resulting free oil is total Rice bran crude oil, calculates demulsification efficiency and gained edible vegetable oil oryzanol content.
The demulsification efficiency and oryzanol content for the emulsion difference breaking method that 1 aqueous enzymatic extraction Rice oil of table is formed
Adjusting pH method is to reach the mesh of the structure and charging property that change interfacial film by changing emulsion system pH environment
, to effectively be demulsified.There is still the obstinate emulsion in part to exist after first demulsification centrifugation, by the way that calcium chloride solution is added, breaks
The double electrical layers of bad egg's white matter promote to occur to assemble between oil droplet to cause to be demulsified.It can be seen that from the data of table 1 using this
The breaking method for inventing offer, which carries out demulsification processing to the emulsion that aqueous enzymatic extraction Rice oil generates, can effectively improve demulsification efficiency,
Oryzanol content also significantly improves in gained Rice bran crude oil simultaneously, has low energy consumption, pollution-free, green and healthy, easy to operate etc.
Advantage.
The above is only a preferred embodiment of the present invention, it is noted that those skilled in the art are come
It says, without departing from the principle of the present invention, several improvement can also be made, these improvement also should be regarded as protection of the invention
Range.
Claims (7)
1. a kind of emulsion breakdown method that aqueous enzymatic extraction Rice oil is formed, which comprises the following steps:
(1) rice bran sample treatment: carry out the rice bran Jing Guo expanding treatment to crushed 40 meshes obtaining rice bran powder;
(2) it is enzymatically treated: deionized water will be added in rice bran powder that step (1) obtains, be tuned into mixed liquor by certain solid-liquid ratio,
Mixed liquor is warming up to 90 DEG C of enzyme deactivation 15min, is cooled to room temperature later.Rice bran pH of mixed is adjusted with the HCL solution of 2mol/L
To 5.0, a certain amount of 1,4 beta-glucanase and plant extract enzyme are added, is digested to obtain first rice bran zymolyte.Use 2mol/L
NaOH solution adjust first rice bran zymolyte pH to 9.0, the alkali carries certain time at 60 DEG C.It is molten with the NaOH of 2mol/L later
Liquid continues to adjust pH to 9.0, adds a certain amount of Acalase2.4L alkali protease, is digested, obtained under certain condition
Final rice bran zymolyte.Obtained rice bran zymolyte is placed in enzyme deactivation 10min in 90 DEG C of water-baths, later under certain condition from
The heart obtains emulsion layer, hydrolysis liquid layer, residual layer;
(3) emulsion breakdown: the emulsion that step (2) is obtained takes out, and stands 12h at 4 DEG C, carries out demulsification processing to it,
It is centrifuged under certain condition, upper layer grease is taken out, lower layer's stubbornness emulsion takes out spare.The obstinate emulsion that will be obtained after centrifugation
Demulsification processing is carried out again, is centrifuged under certain condition, upper layer free oil is taken out, and the sum of the resulting free oil that is demulsified twice is
Total Rice bran crude oil.
2. the emulsion breakdown method that a kind of aqueous enzymatic extraction Rice oil according to claim 1 is formed, which is characterized in that
Solid-liquid ratio, that is, rice bran weight in the step (2): deionized water weight 1: (5~8).
3. the emulsion breakdown method that a kind of aqueous enzymatic extraction Rice oil according to claim 1 is formed, which is characterized in that
The amount of 1,4 beta-glucanase and plant extract enzyme is 1%~5% in the step (2), and the two ratio is 1: (1~3), enzymatic hydrolysis condition
It is 50 DEG C, 1h~3h.
4. the emulsion breakdown method that a kind of aqueous enzymatic extraction Rice oil according to claim 1 is formed, which is characterized in that
The alkali carries time is 5min~60min in the step (2).
5. the emulsion breakdown method that a kind of aqueous enzymatic extraction Rice oil according to claim 1 is formed, which is characterized in that
The amount of Acalase2.4L alkali protease is 1%~5% in the step (2), and enzymatic hydrolysis condition is 60 DEG C, 2h~3h.
6. the emulsion breakdown method that a kind of aqueous enzymatic extraction Rice oil according to claim 1 is formed, which is characterized in that
The centrifugal condition of the step (2) and step (3) is 6000r/min~10000r/min, 10min~30min.
7. the emulsion breakdown method that a kind of aqueous enzymatic extraction Rice oil according to claim 1 is formed, which is characterized in that
Demulsification processing twice is respectively to adjust emulsion pH to 3.5~5.5, at 40 DEG C~80 DEG C, water bath processing in the step (3)
1h~3h;Add 0.03mol/L~0.1mol/L calcium chloride solution, at 60 DEG C~80 DEG C, water bath processing 10min~90min.
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