CN104450156B - The method of grease and a kind of fructus cannabis matter beverage in a kind of aqueous enzymatic extraction fructus cannabis - Google Patents
The method of grease and a kind of fructus cannabis matter beverage in a kind of aqueous enzymatic extraction fructus cannabis Download PDFInfo
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- 241000218236 Cannabis Species 0.000 title claims abstract description 93
- 235000013361 beverage Nutrition 0.000 title claims abstract description 44
- 239000004519 grease Substances 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 26
- 238000000605 extraction Methods 0.000 title claims abstract description 24
- 230000002255 enzymatic effect Effects 0.000 title claims abstract description 23
- 239000007791 liquid phase Substances 0.000 claims abstract description 42
- 102000004190 Enzymes Human genes 0.000 claims abstract description 35
- 108090000790 Enzymes Proteins 0.000 claims abstract description 35
- 238000001125 extrusion Methods 0.000 claims abstract description 35
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 235000003599 food sweetener Nutrition 0.000 claims abstract description 16
- 239000003765 sweetening agent Substances 0.000 claims abstract description 16
- 239000002994 raw material Substances 0.000 claims abstract description 12
- 239000000284 extract Substances 0.000 claims abstract description 11
- 238000001914 filtration Methods 0.000 claims abstract description 11
- 238000005119 centrifugation Methods 0.000 claims abstract description 8
- 239000004365 Protease Substances 0.000 claims description 25
- 108091005804 Peptidases Proteins 0.000 claims description 23
- 239000003513 alkali Substances 0.000 claims description 23
- 102000035195 Peptidases Human genes 0.000 claims description 20
- 235000019419 proteases Nutrition 0.000 claims description 19
- 239000007788 liquid Substances 0.000 claims description 17
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 claims description 16
- 102000004169 proteins and genes Human genes 0.000 claims description 15
- 108090000623 proteins and genes Proteins 0.000 claims description 15
- 238000003756 stirring Methods 0.000 claims description 11
- 108010056079 Subtilisins Proteins 0.000 claims description 9
- 102000005158 Subtilisins Human genes 0.000 claims description 9
- 239000002245 particle Substances 0.000 claims description 7
- 239000000047 product Substances 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 5
- 101710093543 Probable non-specific lipid-transfer protein Proteins 0.000 claims description 4
- 229930006000 Sucrose Natural products 0.000 claims description 4
- 150000001875 compounds Chemical class 0.000 claims description 4
- 239000000835 fiber Substances 0.000 claims description 4
- 230000007935 neutral effect Effects 0.000 claims description 4
- 239000012071 phase Substances 0.000 claims description 4
- 239000005720 sucrose Substances 0.000 claims description 4
- 230000009471 action Effects 0.000 claims description 3
- 230000008859 change Effects 0.000 claims description 3
- 239000003337 fertilizer Substances 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 239000012467 final product Substances 0.000 claims description 2
- 108010043393 protease N Proteins 0.000 claims description 2
- 125000000185 sucrose group Chemical group 0.000 claims description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 3
- 241000894006 Bacteria Species 0.000 claims 1
- 239000005715 Fructose Substances 0.000 claims 1
- 229930091371 Fructose Natural products 0.000 claims 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims 1
- 230000001954 sterilising effect Effects 0.000 abstract description 9
- 241000196324 Embryophyta Species 0.000 abstract description 7
- 239000003205 fragrance Substances 0.000 abstract description 7
- 244000025254 Cannabis sativa Species 0.000 abstract description 5
- 235000012766 Cannabis sativa ssp. sativa var. sativa Nutrition 0.000 abstract description 5
- 235000012765 Cannabis sativa ssp. sativa var. spontanea Nutrition 0.000 abstract description 5
- 235000009120 camo Nutrition 0.000 abstract description 5
- 235000005607 chanvre indien Nutrition 0.000 abstract description 5
- 239000011487 hemp Substances 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 4
- 239000000654 additive Substances 0.000 abstract description 3
- 230000000996 additive effect Effects 0.000 abstract description 3
- 238000004064 recycling Methods 0.000 abstract description 3
- 239000002893 slag Substances 0.000 abstract 2
- 239000003921 oil Substances 0.000 description 49
- 235000019198 oils Nutrition 0.000 description 49
- 229940088598 enzyme Drugs 0.000 description 24
- 239000000463 material Substances 0.000 description 21
- 239000008157 edible vegetable oil Substances 0.000 description 15
- 235000018102 proteins Nutrition 0.000 description 14
- 230000033228 biological regulation Effects 0.000 description 11
- 238000006911 enzymatic reaction Methods 0.000 description 9
- 230000000694 effects Effects 0.000 description 8
- 238000000227 grinding Methods 0.000 description 6
- 239000000839 emulsion Substances 0.000 description 5
- 235000021568 protein beverage Nutrition 0.000 description 5
- 239000003643 water by type Substances 0.000 description 5
- 108010064851 Plant Proteins Proteins 0.000 description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 4
- 238000001816 cooling Methods 0.000 description 4
- 238000004945 emulsification Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 235000019197 fats Nutrition 0.000 description 4
- 238000011049 filling Methods 0.000 description 4
- 235000021118 plant-derived protein Nutrition 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 108090000526 Papain Proteins 0.000 description 3
- 235000001014 amino acid Nutrition 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 235000019834 papain Nutrition 0.000 description 3
- 229940055729 papain Drugs 0.000 description 3
- 239000004475 Arginine Substances 0.000 description 2
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 description 2
- 235000020661 alpha-linolenic acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 2
- 239000002585 base Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 2
- 238000000265 homogenisation Methods 0.000 description 2
- 229960004488 linolenic acid Drugs 0.000 description 2
- KQQKGWQCNNTQJW-UHFFFAOYSA-N linolenic acid Natural products CC=CCCC=CCC=CCCCCCCCC(O)=O KQQKGWQCNNTQJW-UHFFFAOYSA-N 0.000 description 2
- 235000021388 linseed oil Nutrition 0.000 description 2
- 239000000944 linseed oil Substances 0.000 description 2
- 235000008935 nutritious Nutrition 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 230000001007 puffing effect Effects 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- 239000011593 sulfur Substances 0.000 description 2
- 229910052717 sulfur Inorganic materials 0.000 description 2
- QWWVBNODQCWBAZ-WHFBIAKZSA-N (2r)-2-amino-3-[(2r)-2-carboxy-2-(methylamino)ethyl]sulfanylpropanoic acid Chemical compound CN[C@H](C(O)=O)CSC[C@H](N)C(O)=O QWWVBNODQCWBAZ-WHFBIAKZSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 101710089042 Demethyl-4-deoxygadusol synthase Proteins 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 101710180012 Protease 7 Proteins 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 239000010779 crude oil Substances 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000036449 good health Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 235000020778 linoleic acid Nutrition 0.000 description 1
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 108010009355 microbial metalloproteinases Proteins 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 239000003531 protein hydrolysate Substances 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 235000019633 pungent taste Nutrition 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- -1 soluble-carbohydrate Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 235000020985 whole grains Nutrition 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/60—Sweeteners
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/04—Pretreatment of vegetable raw material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The method that the present invention disclose grease in a kind of aqueous enzymatic extraction fructus cannabis, the method includes extrusion, extracts fire numb albumen and grease, digests and the step of the enzyme that goes out is centrifuged；Free oil, liquid phase and fructus cannabis slag are obtained after centrifugation, fructus cannabis oil is obtained after free oil filtering, dewater treatment, slag is used as feedstuff.The present invention also provides a kind of fructus cannabis beverage, and the raw material of the beverage is the recycling to the liquid phase accessory substance of grease in aqueous enzymatic extraction fructus cannabis, is liquid phase by adding water, sweetener after filtering, is obtained after homogeneous, sterilizing.3%~8%, compared with other commercially available hemp seed beverages, the additive amount of this product is substantially reduced the protein content of this product, with natural plant fragrance.
Description
Technical field
Technical field the present invention relates to extract processing.More particularly, to grease in a kind of aqueous enzymatic extraction fructus cannabis
Method and a kind of fructus cannabis matter beverage.
Background technology
Fructus cannabis is product of the fiery numb seed by shelling, and fat content 43%~48% is also left containing 30% beyond oil removing
Right protein, 5% or so sugared and abundant calcium, phosphorus, iron, zinc, manganese etc..Unsaturated fatty acid content surpasses in fructus cannabis oil
85%, especially linolenic acid content are crossed higher, more than 16%.The ratio (ω -6) of linoleic acid plus linolenic acid：(ω -3) is approached
3：1, meet internationally recognized nutritive proportion standard；Fructus cannabis oil can improve the enzyme activities such as SOD/GSH-Px, remove DPPH certainly
Olive oil has been exceeded by the ability of base, a kind of oxidation resistant dietary sources have been suitable as.The primary amino acid of fructus cannabis matter
It is arginine, histidine, sulfur-containing amino acid, arginine, histidine are extremely important in the children growth phase, the first in sulfur-containing amino acid
Methyllanthionine and cysteine be produce biology enzyme necessary to, fructus cannabis is one of quality plant albumen source.
Aqueous enzymatic method carries oil as a kind of biological oil extracting methods, with its mild condition, efficiency high, safety simple to operate, oil
The advantages of height, feature of environmental protection, extracts the focus of research as recent year grease.It is more using total during aqueous enzymatic extraction
Oil extract rate evaluates oil extracting rate, i.e.,：Percent of total oil (%)=(always having quality in the total oil quality-residue of raw material) * 100%/original
Total oil quality in material, due to emulsion presence and cannot thoroughly be demulsified, general actual free edible vegetable oil yield (free oil yield
The total oil quality of (%)=free edible vegetable oil quality 100%/raw materials of *) it is significantly less than percent of total oil.Thus, emulsification is reduced, improve clear
Oily yield is the very important link of oil extraction by enzymatic processing.
Used as a kind of pretreatment technology of vegetable oil material, principle is using broken teeth spiral shell in extruder to extruding and puffing technology
The combination of extrusion of rotation and shear pin, the mechanism such as rub, shear, and frictional heat and penetrating steam hyther,
Material is set to be in instantaneous high-temperature hyperbaric environment, so as to destroy cell tissue structure.The moment of nib, pressure wink are squeezed out in material
Between discharge, moisture evaporated rapidly, and cell tissue is destroyed again.As the pretreatment technology of oil extraction by enzymatic processing, can be with enzyme successively
Synergy, grease discharges and is easy to extract purpose in playing promotion oil plant.
The method that patent CN102816631A discloses a kind of acid system extrusion aqueous enzymatic extraction fire flaxseed oil fat, its
Material is sprayed with the sulfuric acid of the 2mol/L of weight of material 2%-5% before extrusion, sulfuric acid can have certain corrosion to equipment, together
When, needing to introduce more alkali in enzymatic hydrolysis system when later use alkali protease is digested, the salt of high content can be to water phase
Quality brings adverse effect；And free oil yield, for index, is not referred to " total oil oil extracting rate " in patent；Enzyme concentration is mixing
Liquid total amount meter, it is larger with enzyme amount, and do not refer to the recycling of accessory substance liquid phase.Patent CN103445259A is direct using fructus cannabis
Protein beverage is made after defibrination enzymolysis, is boiled by whole grain, is beaten, three links of colloid mill fine grinding make proteolytic, but
The amount of solubilising protein and indefinite in final liquid phase.Patent CN101828739A discloses a kind of white fungus drink made from hemp seed food
And preparation method thereof, it is the work for adding white fungus to play thickener on the basis of the conventional direct making beverage using fructus cannabis
With, in preparation process, the filtrate that fructus cannabis is beaten into filtering is allocated, and the content of protein in filtrate is not referred to yet, and
Most nutriment fat and protein etc. are remained in residue, are the significant wastages to the overall utilization of resources.
The small oil plant industry such as fructus cannabis is set to develop in a healthy way, it is necessary to pay attention to the overall processing benefit of resource.Accordingly, it would be desirable to carry
For a kind of method of grease in aqueous enzymatic extraction fructus cannabis that can at utmost ensure free oil yield, while one kind can also be provided
Liquid phase is recycled and is fabricated to protein beverage, it is to avoid fat content height causes the not good fructus cannabis of mouthfeel and stability
Matter beverage.
The content of the invention
It is an object of the present invention to provide a kind of method of grease in aqueous enzymatic extraction fructus cannabis, the method can with gram
The clothes emulsification that aqueous enzymatic method is present in the prior art is serious, is demulsified cumbersome, edible vegetable oil yield is low, and the problem of liquid phase utilizes insufficient should
Method is a kind of Oil Extraction by Enzymatic Processing of the edible vegetable oil yield high without emulsification or microemulsified.
It is another object of the present invention to provide a kind of fructus cannabis matter beverage, the raw material of the beverage is to aqueous enzymatic method
The recycling of the liquid phase accessory substance of grease in fructus cannabis is extracted, and protein is rich in liquid phase, therefore the albumen of this product contains
3%~5%, compared with other commercially available hemp seed beverages, the additive amount of this product is substantially reduced amount, with natural plants
Fragrance.
To reach above-mentioned purpose, the present invention uses following technical proposals：
The method of grease, comprises the following steps in a kind of aqueous enzymatic extraction fructus cannabis：
(1) extrusion：Extrusion will be carried out after fructus cannabis coarse crushing；
(2) fire fiber crops albumen and grease body are extracted：Fructus cannabis after extrusion is crushed, water is added, mixed liquor is obtained, used
The pH of dilute alkaline soln regulation mixed liquor is stirred to 8.0~8.5, at 50~65 DEG C and is extracted 20~40min；
Protein in fructus cannabis is mainly globulin, is soluble in dilute alkaline soln, and the dissolving of protein can promote
The release of oil-feed, improves the recovery rate of grease, but pH is unsuitable too high, base amount cross the color and luster of DDGS in conference influence system,
Increase the salinity in liquid phase, be unfavorable for that later-stage utilization liquid phase prepares protein beverage.
(3) digest：Stirring adjusts the most suitable action pH of pH of mixed to enzyme after extracting, add the fructus cannabis after extrusion
The alkali protease of weight 0.5%~2.0%, digests 2~6h；Add the fructus cannabis weight 0.5%~1.5% after extrusion
Neutral proteinase, enzymolysis 2~4 hours after obtain enzymolysis liquid；
Alkali protease is directed to fructus cannabis aqueous enzymatic method and carries the preferable protein restriction endonuclease of oily using effect, but is used alone
The enzyme easily forms emulsion layer, with neutral proteinase compound use, almost without emulsion layer, can greatly improve edible vegetable oil and total oil extract
Rate.Because alkali protease is different to the action site of protein from neutral proteinase, when alkali protease is used alone
Still with the presence of macro-molecular protein, the formation of stable emulsion is easily facilitated as surfactant, use neutral proteinase pair
Protein C-the end or N- ends of basic protein enzyme effect are degraded herein, reduce the molecular weight and flexible journey of protein molecule
Degree, reduces its emulsifying capacity, such that it is able to improve free oil yield.
Control alkali protease and neutral protein enzyme dosage and enzymolysis time, had both made various enzymes give full play to enzymolysis effect
Power, grease is discharged from wrapped protein, and cost can be saved again, reduces energy consumption.
(4) enzyme that goes out is centrifuged：Enzymolysis liquid is carried out into 5~10min of destroy the enzyme treatment at 90~95 DEG C, is then centrifuged for separating, obtained
Free oil, liquid phase and the part of residue three；Obtained final product after free oil dewater treatment.
The enzyme that goes out centrifugation makes alkali protease and neutral proteinase inactivation in system, prevents that protein from excessively hydrolyzing, it is ensured that
The mouthfeel of follow-up beverage.Residue can be as feedstuff.
Preferably, step (1) described coarse crushing is that fructus cannabis is crushed the particle for mesh number >=10 mesh.
Preferably, in step (1) described extrusion pressing puffing process, bulking machine rotating speed 60-90rpm, feeding machine rotating speed are controlled
160-200rpm, 5-70 DEG C of bulking machine bushing temperature, it is 5.0%~10.0% to control expanded rear fructus cannabis moisture content.
Because fructus cannabis oil content and protein content are higher, increase water content after material water suction when steam is larger,
Proterties is sticky easily to be caused stifled chamber and influences expansion effect, therefore keeps suitable quantity of steam particularly important；Feeding rotating speed is too fast, swollen
Change machine rotating speed causes spout putty when slower, otherwise makes expanded discharging intermittent, thus the rotating speed coordinated just can guarantee that it is swollen
That changes is smoothed out；Bushing temperature is too high, can be denatured protein in material, and oil is carried to later stage aqueous enzymatic method and produces unfavorable shadow
Ring.
Preferably, the fructus cannabis after step (2) will be expanded is crushed to average grain diameter≤50 μm；It is fire fiber crops to add the weight of water
3-8 times of benevolence weight；
The fineness of crushing material influences the oil extracting rate of aqueous enzymatic method, and powder particle diameter is smaller, and oil extracting rate is higher, but crushes meticulous,
Emulsion layer is serious, increases the difficulty of demulsification.
It is highly preferred that the weight for adding water is 4-6 times of fructus cannabis weight.
Preferably, step (3) described alkali protease be selected from food-grade Alcalase 2.4L, FoodPro protease,
One or more in 2709 alkali proteases or papain；
Above-mentioned alkali protease optimal pH scope is respectively：Alcalase 2.4L6.5~8.5, FoodPro proteinase 9s~
10,2709 alkali proteases 8.5~9.5, papain 8.0~9.5；
The neutral proteinase is selected from food-grade albumen enzyme Alphalase, compound fertilizer production or Neutrase
It is one or more；
Above-mentioned neutral proteinase optimal pH scope is respectively：Food-grade albumen enzyme Alphalase6~8, composite flavor albumen
Enzyme 5.0~7.0, Neutrase5.5~7.5；
Preferably, the alkali protease is Alcalase 2.4L, and neutral proteinase is Alphalase；
Preferably, the basic protein enzyme dosage is the 0.5%~1.5% of fructus cannabis weight, and neutral protein enzyme dosage is
The 0.5%~1% of fructus cannabis weight.
Preferably, step (4) described centrifugation uses three-phase centrifuge or horizontal centrifuge.
A kind of fructus cannabis matter beverage, the primary raw material of the fructus cannabis beverage is aqueous enzymatic method of the present invention
The liquid phase obtained in the method for extracting grease in fructus cannabis.
Preferably, protein content is 3%-8% in the fructus cannabis beverage.
Preferably, sweetener and water are also included in the raw material of the fructus cannabis beverage, it is preferable that the sweetener
Weight is the 1%-10% of the liquid phase weight；The weight of the water is 0-1 times of liquid phase.The purpose for adding water is, directly out
Liquid phase in protein content it is too high, add water and not only can suitably dilute, reach beverage stabilization effect, again can be to a certain degree
Upper increase product yield.
The sweetener is selected from sucrose or FOS.
The present invention also provides a kind of preparation method of fructus cannabis beverage, is by oil in above-mentioned aqueous enzymatic extraction fructus cannabis
The liquid phase obtained in the method for fat obtains product fructus cannabis drink by adding water, sweetener after filtering after homogeneous, sterilizing
Material.
Preferably, homogenization pressure is 20~50Mpa；Sterilising conditions are 15min at 121 DEG C.
During aqueous enzymatic method carries oil, substantial amounts of protein hydrolysate, soluble-carbohydrate, mineral matter etc. are dissolved in liquid
Phase, the drink of preparation is nutritious, to promoting health with multiple efficacies, is good health function plant nutritious beverage.
During allotment, adding sweetener FOS makes beverage mouthfeel more preferably, and homogenization can improve the stability of beverage.
The measure foundation of indices in the present invention：
Oil quality in edible vegetable oil yield (%)=edible vegetable oil quality 100%/raw materials of *
Oil quality in total oil yield (%)=(oil quality in oil quality-residue in raw material) * 100%/raw material.
Beneficial effects of the present invention are as follows：
The present invention does not use organic solvent compared with organic solvent oil extracting process, belongs to environmentally friendly, and quality of crude oil
It is good, refining link is reduced, save equipment investment；Compared with oil extraction by pressing, albumen can be recycled while oil is ensured
Matter, at utmost develops the value of the resource.
Compared with other fructus cannabis aqueous enzymatic methods put forward oily patent, enzyme concentration is calculated the present invention with the percentage of raw material, uses enzyme amount
It is few, reduce financial cost；Digested using alkali protease+neutral proteinase, greatly reduced the degree of emulsification, dropped
Low demulsification operation burden, and improve free oil yield；Vegetable protein beverage is directly prepared using liquid phase, accessory substance is improved
Utilization rate.
It is of the invention compared with other drink made from hemp seed, be by fructus cannabis content highest grease extract after to rich in hydrolysis
The utilization of the liquid phase of albumen.Compared with other commercially available hemp seed beverages, additive amount is substantially reduced, protein content 3%~
8%, containing natural plant fragrance, it is suitable to different crowd and drinks.
Brief description of the drawings
Specific embodiment of the invention is described in further detail below in conjunction with the accompanying drawings.
Fig. 1 shows the process chart of the embodiment of the present invention 1.
Embodiment
In order to illustrate more clearly of the present invention, the present invention is done further with reference to preferred embodiments and drawings
It is bright.Similar part is indicated with identical reference in accompanying drawing.It will be appreciated by those skilled in the art that institute is specific below
The content of description is illustrative and be not restrictive, and should not be limited the scope of the invention with this.
Embodiment 1
2kg shelling fructus cannabis is weighed, extrusion is carried out after coarse crushing, mould aperture 8mm, bulking machine rotating speed 90rpm feed
Material machine rotating speed 200rpm, bushing temperature is 60 DEG C, and material moisture is 9.6% after extrusion；
The fructus cannabis after extrusion is taken, size-reduced machine coarse crushing is milled to particle diameter≤50 μm after grinding mill, weighed
1kg, adds 4L deionized waters, regulation system pH to 8.5 after stirring, and temperature is 60 DEG C, extracts 35min；
Then regulation system pH to 8.5, adds alkali protease Alcalase 2.4L (after addition is extrusion
Fructus cannabis weight 2%), after effect 4 hours, adding neutral proteinase Alphalase, (addition is the fire fiber crops after extrusion
Benevolence weight 0.5%), stirring enzymolysis 3h obtain enzymolysis liquid；
Enzymolysis liquid temperature will be obtained to rise to 90 DEG C and keep 5min to go out enzyme, centrifugation 15min under 4000rpm, enzymolysis liquid
It is divided into three layers of edible vegetable oil, liquid phase and residue；Edible vegetable oil yield is 86.12%, and total oil yield is 89.44%.
Gained liquid phase is through filtering, addition sweetener (addition is the 5% of liquid weight), the water of equivalent, under 30Mpa
Matter (2min/ times, 2 times), filling, 121 DEG C of sterilizing 15min, obtain beverage after cooling.Plant protein content 4.8% in beverage, drink
Material bakes and banks up with earth fragrance with distinctive.Protein content is less than 3% in general vegetable protein beverage, and national sector standard is usually
More than 0.6%.
Embodiment 2
3kg shelling fructus cannabis is weighed, extrusion is carried out after coarse crushing, mould aperture 6mm, bulking machine rotating speed 70rpm feed
Material machine rotating speed 175rpm, bushing temperature is 55 DEG C, and material moisture is 6.2% after extrusion；
The fructus cannabis after extrusion is taken, size-reduced machine coarse crushing is milled to particle diameter≤50 μm after grinding mill, weighed
500g, adds 1.5L deionized waters, regulation system pH to 8.0 after stirring, and temperature is 60 DEG C, extracts 30min；
Regulation system pH to 8.5, adds the alkali protease Alcalase of the fructus cannabis weight 1% after extrusion
The neutral proteinase Alphalase of fructus cannabis weight 1% after 2.4L and extrusion, low rate mixing enzymolysis 6h；
System temperature is risen to 95 DEG C and keeps 5min to go out enzyme, 15min is centrifuged under 4500rpm, enzymolysis liquid is divided into clearly
Three layers of oil, liquid phase and residue；Edible vegetable oil yield is 90.69%, and total oil yield is 91.06%.
Gained liquid phase is through filtering, addition sweetener (addition is the 3% of liquid phase weight), the water of liquid phase equivalent, in 20Mpa
Lower homogeneous (3min/ times, 3 times), 121 DEG C sterilizing etc. processing after obtain beverage.Plant protein content 6.9% in beverage, beverage vessel
Have and distinctive bake and bank up with earth fragrance.
Embodiment 3
5kg shelling fructus cannabis is weighed, extrusion is carried out after coarse crushing, mould aperture 6mm, bulking machine rotating speed 80rpm feed
Material machine rotating speed 188rpm, bushing temperature is 70 DEG C, and material moisture is 7.8% after extrusion；
The fructus cannabis after extrusion is taken, size-reduced machine coarse crushing is milled to particle diameter≤50 μm after grinding mill, weighed
1.5kg, adds 7.5L deionized waters, regulation system pH to 8.0 after stirring, and temperature is 60 DEG C, extracts 30min；
Regulation system pH to 9.0, in the alkali protease Alcalase2.4L and 1% of addition fructus cannabis weight 1.5%
Property protease A lphalase, low rate mixing enzymolysis 5h；
System temperature is risen to 95 DEG C and keeps 5min to go out enzyme, centrifugation 15min under 4500rpm, enzymolysis liquid is divided into clearly
Three layers of oil, liquid phase and residue；Edible vegetable oil yield is 89.21%, and total oil yield is 92.40%.
Liquid phase through filtering, add sweetener 7%, under 35Mpa homogeneous (3min/ time, 3 times), 121 DEG C sterilizing etc. process after
Obtain beverage.Plant protein content 7.6% in beverage, beverage has distinctive bakes and banks up with earth fragrance
Embodiment 4
2kg shelling fructus cannabis is weighed, extrusion is carried out after coarse crushing, mould aperture 10mm, bulking machine rotating speed 70rpm,
Feeding machine rotating speed 175rpm, bushing temperature is 65 DEG C, and material moisture is 9.6% after extrusion；
The fructus cannabis after extrusion is taken, size-reduced machine coarse crushing is milled to particle diameter≤50 μm after grinding mill, weighed
1kg, adds 5L deionized waters, regulation system pH to 8.5 after stirring, and temperature is 50 DEG C, extracts 40min；
Then regulation system pH to 8.5, adding alkali protease Alcalase 2.4L, (addition is fructus cannabis weight
2%) after, acting on 4 hours, neutral proteinase Alphalase (addition is the 0.5% of fructus cannabis weight), stirring enzymolysis are added
3h obtains enzymolysis liquid；
Enzymolysis liquid temperature will be obtained to rise to 90 DEG C and keep 5min to go out enzyme, using horizontal centrifuge centrifugation, enzymolysis liquid
It is divided into three layers of edible vegetable oil, liquid phase and residue；Edible vegetable oil yield is 90.38%, and total oil yield is 93.25%.
Gained liquid phase is equal under 30Mpa through filtering, addition sweetener FOS (addition is the 1% of liquid phase weight)
Matter (2min/ times, 2 times), filling, 121 DEG C of sterilizing 15min, obtain beverage after cooling.
Embodiment 5
2kg shelling fructus cannabis is weighed, extrusion is carried out after coarse crushing, mould aperture 8mm, bulking machine rotating speed 60rpm feed
Material machine rotating speed 160rpm, bushing temperature is 60 DEG C, and material moisture is 9.6% after extrusion；
The fructus cannabis after extrusion is taken, size-reduced machine is crushed and is milled to average grain diameter≤50 μm after grinding mill；Weigh
1kg, adds 8L deionized waters, regulation system pH to 8.5 after stirring, and temperature is 65 DEG C, extracts 20min；
Then regulation system pH to 8.5, adding alkali protease Alcalase 2.4L, (addition is fructus cannabis weight
2%) after, acting on 4 hours, neutral proteinase Alphalase (addition is the 0.5% of fructus cannabis weight), stirring enzymolysis are added
3h obtains enzymolysis liquid；
Enzymolysis liquid temperature will be obtained to rise to 90 DEG C and keep 10min to go out enzyme, using three-phase centrifuge centrifugation point, enzymolysis liquid point
For three layers of edible vegetable oil, liquid phase and residue；Edible vegetable oil yield is 88.67%, and total oil yield is 91.73%.
Gained liquid phase is through filtering, addition sweetener sucrose (addition is the 10% of liquid phase weight), the homogeneous under 50Mpa
(2min/ times, 2 times), filling, 121 DEG C of sterilizing 15min obtain beverage after cooling.Plant protein content 4.97% in beverage, drink
Material bakes and banks up with earth fragrance with distinctive
Embodiment 6-8
With embodiment 1, it the difference is that only：The alkali protease is respectively FoodPro protease, 2709 alkalescence
Protease or papain.
Embodiment 9-11
With embodiment 1, it the difference is that only：The neutral proteinase be respectively food-grade albumen enzyme Alphalase,
Compound fertilizer production or protease N eutrase.
Embodiment 10
Using a kind of fiery flaxseed oil fat of acid system extrusion aqueous enzymatic extraction disclosed in CN102816631A in the prior art
Liquid phase, by liquid phase through filtering, add sweetener sucrose (addition is the 10% of liquid phase weight), the homogeneous under 50Mpa
(2min/ times, 2 times), filling, 121 DEG C of sterilizing 15min obtain beverage after cooling.Beverage produces flocculation, it is impossible to equal as stabilization
One drink system, and slightly bad pungent taste.
Obviously, the above embodiment of the present invention is only intended to clearly illustrate example of the present invention, and is not pair
The restriction of embodiments of the present invention, for those of ordinary skill in the field, may be used also on the basis of the above description
To make other changes in different forms, all embodiments can not be exhaustive here, it is every to belong to this hair
Obvious change that bright technical scheme is extended out changes row still in protection scope of the present invention.
Claims (11)
1. in a kind of aqueous enzymatic extraction fructus cannabis grease method, it is characterised in that comprise the following steps：
（1）Extrusion：It is the particle of mesh number >=10 mesh by fructus cannabis coarse crushing, then carries out extrusion；Wherein, control swollen
Change machine rotating speed 60-90rpm, feeding machine rotating speed 160-200 rpm, 5-70 DEG C of bulking machine bushing temperature control expanded rear fructus cannabis
Moisture content is 5.0%-10.0%；
（2）Extract fire fiber crops albumen and grease body：Fructus cannabis after extrusion is crushed to average grain diameter≤50 μm, added water, plus
It is 3-8 times of fructus cannabis weight to enter the weight of water, obtains mixed liquor, and the pH of mixed liquor is adjusted to 8.0-8.5 with dilute alkaline soln,
20-40 min are extracted in stirring at 50-65 DEG C；
（3）Enzymolysis：Stirring adjusts the most suitable action pH of pH of mixed to enzyme after extracting, add the fructus cannabis weight after extrusion
The alkali protease of 0.5%-2.0%, digests 2-6h；Add the neutral protein of the fructus cannabis weight 0.5%-1.5% after extrusion
Enzymolysis liquid is obtained after enzyme, enzymolysis 2-4h；
（4）The enzyme that goes out is centrifuged：Enzymolysis liquid is carried out into destroy the enzyme treatment 5-10 min at 90-95 DEG C, is then centrifuged for separating, dissociated
Oil, liquid phase and the part of residue three；Obtained final product after free oil dewater treatment.
2. in aqueous enzymatic extraction fructus cannabis according to claim 1 grease method, it is characterised in that：Step（2）In, plus
The weight for entering water is 4-6 times of fructus cannabis weight.
3. in aqueous enzymatic extraction fructus cannabis according to claim 1 grease method, it is characterised in that：Step（3）It is described
Alkali protease is selected from the one kind or many in food-grade Alcalase 2.4L, FoodPro protease or 2709 alkali proteases
Kind；The neutral proteinase is selected from food-grade albumen enzyme Alphalase, compound fertilizer production or protease N eutrase
One or more.
4. the method for grease in aqueous enzymatic extraction fructus cannabis according to claim 3, it is characterised in that：The basic protein
Enzyme is Alcalase 2.4L, and neutral proteinase is Alphalase.
5. in aqueous enzymatic extraction fructus cannabis according to claim 1 grease method, it is characterised in that：The basic protein
Enzyme dosage is the 0.5%-1.5% of fructus cannabis weight, and neutral protein enzyme dosage is the 0.5%-1.0% of fructus cannabis weight.
6. the method for grease in aqueous enzymatic extraction fructus cannabis according to claim 1, it is characterised in that：Step（4）It is described
Centrifugation uses three-phase centrifuge or horizontal centrifuge.
7. a kind of fructus cannabis beverage, it is characterised in that：The primary raw material of the fructus cannabis beverage is claim 1-6
The liquid phase obtained in aqueous enzymatic extraction fructus cannabis described in any one in the method for grease；Albumen in the fructus cannabis beverage
Matter content is 3%-8%.
8. fructus cannabis beverage according to claim 7, it is characterised in that：In the raw material of the fructus cannabis beverage
Also include sweetener and water.
9. fructus cannabis beverage according to claim 8, it is characterised in that：The weight of the sweetener is the liquid phase
The 1-10% of weight；The weight of the water is 0-1 times of liquid phase.
10. fructus cannabis beverage according to claim 8, it is characterised in that：The sweetener is selected from sucrose or oligomeric
Fructose.
11. the preparation method of fructus cannabis beverage as claimed in claim 7, it is characterised in that：Claim any one of 1-6
The liquid phase obtained in described aqueous enzymatic extraction fructus cannabis in the method for grease adds water, sweetener after filtering, homogeneous, goes out
Product fructus cannabis beverage is obtained after bacterium.
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