CN109909513A - A kind of method and purposes with comospore Glochidion puberum biosynthesis nano silver particles - Google Patents
A kind of method and purposes with comospore Glochidion puberum biosynthesis nano silver particles Download PDFInfo
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- CN109909513A CN109909513A CN201910344232.1A CN201910344232A CN109909513A CN 109909513 A CN109909513 A CN 109909513A CN 201910344232 A CN201910344232 A CN 201910344232A CN 109909513 A CN109909513 A CN 109909513A
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Abstract
The invention discloses the biological synthesis method and purposes of a kind of environmentally friendly nano-silver ionic, synthetic method, which includes: that the acquisition fresh blade of comospore Glochidion puberum is clean, dries；Chopping comospore Glochidion puberum blade heat and extract filter after reacted under dark condition with silver nitrate solution and become brown to solution nano silver can be obtained.This reaction synthesis process is low to environmental requirement, prepares that dispersion performance is good, and partial size is medium, and particle size distribution range is narrow, harmless to plant growth, and form is spherical in shape or the nano-silver ionic of almost spherical.The nano silver of experiment discovery this method production has repellent action to termite, which is widely used to termite control in wood industry.
Description
Technical field
The present invention relates to a kind of methods of cheap, efficient environmentally friendly nano silver particles of biosynthesis, that is, utilize hair
Method and synthesizing nano-silver material the answering as Wood protecting agent in timber industry of fruit Glochidion puberum blade synthesizing nano-silver
With.
Background technique
Nano silver (silver nanoparticles) refers at least one-dimensional within the scope of 1-100nm in length, width and height three dimensionality
Silver particles.Because it has the characteristics that has a broad antifungal spectrum, is not likely to produce drug resistance and good biocompatibility, curing extensively
Treatment field, daily household field and field of food etc. are used as preservative.It also serves as probe and is applied to biological order-checking.
The synthesis of nano silver includes physical method, chemical method, biological synthesis method etc..Physical method needs high temperature and pressure
And expensive equipment could synthesizing nano-silver.Chemical reagent used in chemical method to a certain extent has human body or environment
Harm, some stabilizers or dispersing agent even have carcinogenicity.With the increase of environmental consciousness in society, find green, safe, low
The nano silver preparation method of energy consumption becomes inevitable.
Biological synthesis process has the characteristics that green, safety, low energy consumption.Used raw material has bacterium, fungi, algae, ferment
Female, actinomyces and some natural plant materials etc..According to the substrate feature of nano particle biosynthesis, it is segmented into two types
Biosystem: a) microorganism extracts and b) plant extracts.
Compared with microorganism is as reducing agent, the method for plant reduction synthesizing nano-silver does not need harsh microculture
Environment, thus operate simpler.Therefore the induction biosynthesis method of nano silver has become the research hotspot of related fields, people in recent years
In succession use different plant extracts synthesizing nano-silvers.Such as using cellulose, orange juice, folium cortex eucommiae, bagasse, blueberry leaf,
Geranium leaves, black tea, Fatsia japonica blade, mangrove bark extracting solution, the plant extraction liquids such as herba lysimachiae extracting solution, firethorn fruit close
At nano silver etc..And each improved place in need of existing various methods.
Patent No. CN103341642B " a kind of cellulose green syt method of nano-silver ionic " needs 180-210 DEG C of high temperature
10-24 hours, energy consumption was higher；The technical disadvantages of patent No. CN103769603B " nano-silver ionic and its synthetic method " are synthesis
Argent grain diameter is 50-150nm.And it is not belonging to nano silver scope more than 100nm, therefore yield is lower, and so big
The microbial resistance effect of grain silver ion is not also verified.Patent No. CN106862590B is " a kind of to utilize Guangxi Du Jingye extract
Biosynthesis nano silver " is needed using photocatalysis, therefore reaction is concentrated mainly on light touching interface, and reaction efficiency is not high.The patent No.
Then reaction step is cumbersome by CN104874810B " a kind of method that bagasse extract green quickly prepares nano silver colloidal sol ".The patent No.
CN104889418B " a kind of biological synthesis method of cube shaped nano silver particles " and patent No. CN104889419B are " a kind of
The biological synthesis method of ball shaped nano silver particles " is limited by raw material, such as the collection problem of sweet orange peel and mangrove bark.The patent No.
CN106513707B " a kind of nano silver antibacterial agent and its preparation process using blueberry leaf extract biosynthesis " needs 90 to take the photograph
Family name's degree is heated to reflux 1 hour, and process compares energy consumption.Patent No. CN103949658B is " a kind of to be closed using Cortex Eucommiae water extract green
At the method for nano silver ", the raw material Bark of Eucommia Ulmoides used is rare medicinal material.
In view of it is above-mentioned based on material of vegetable origin be reducing agent synthesizing nano-silver method limitation, screening more blazon and
Cheap plant substrates and Optimizing Flow, the synthesis of nano silver is carried out with easier method, is had great importance.
Comospore Glochidion puberum is Euphorbiaceae evergreen shrubs plant.It is distributed widely in Jiangsu, Fujian, tw Taiwan, Hunan, wide
The provinces and regions such as east, Hainan, Guangxi, Guizhou and Yunnan.It is widely distributed.Complete stool or root, leaf can hyoscine, have solution paint poison, convergence
The effect of antidiarrheal, removing dampness to stop itchin.Modern study, gallic acid and its derivative contained by the plant all have antibacterial, it is antitumor,
Anti-oxidant and anti-HBV effect isoreactivity can be used to treat dysentery, raw lacquer allergy, paddy-field dermatitis, skin are controlled in leaf external application
Itch, nettle rash, eczema, exfoliative dermatitis.
It is raw material that comospore Glochidion puberum leaf extract and silver nitrate widely distributed, simple and easy to get are selected in this research, raw
Object synthesis has the nano silver of good antibacterial activity.
Summary of the invention
It is an object of the invention to solve the shortcoming of prior art, provide a kind of by rich in comospore Glochidion puberum blade
Rich polyphenoils is reducing agent, with low cost, the method for environmental-friendly mode synthetic environment friendly nano silver particles, and
It was found that new opplication of such nano-silver ionic in wood industry, i.e., for carrying out repellent to termite, thus as wood protection
The application of agent.The method the following steps are included:
A method of with comospore Glochidion puberum blade biosynthesis nano silver particles, comprising the following steps:
1) fresh, healthy comospore puberulous glochidion branchlet and leaf is acquired, blade is washed with distilled water, to remove dust and other impurities；
It is dried after cleaning spare；
2) fresh leaf that 10 grams are cleaned and dried is cut into small pieces with scissors and is put into sterile glass burning by the preparation of leaf extract
Cup, is added 100 milliliters of distilled water, heats and extracted, through Whatmann1 filter paper mistake after then cooling down leaf extract
Filter, filtered extracting solution is in 4 DEG C-preservation；
3) fresh nitric acid silver AgNO3, concentration 1mol/L are prepared with sterile distilled water with reacting for AgNO3, in sterile cone
The 100mL AgNO3 prepared is added in shape flask, above-mentioned leaf extract is added, then conical flask is covered with aluminium foil, is placed on
Under dark condition, standing or the whirling vibration in rotor-vibrating screen, until solution becomes brown.
Further, extraction temperature range described in step 2 is 10-100 DEG C.
Preferably, the silver nitrate of every 100ml concentration 1mM described in step 3 can be carried out with 2.5-15ml leaf extract
React nitric acid synthesis silver.
Preferably, synthesis reaction temperature described in step 3 is 20-40 DEG C.
Preferably, the oscillation frequency range of rotor-vibrating screen described in step 3 is 100-500rpm.
It further, is spherical shape with the sodium rice silver that this method obtains, average diameter is 25.6 nanometers, in knot under natural conditions
It is crystalline.
The nano silver particles that the present invention goes back Sustainable use method synthesis of the invention are used for the application of Wood protecting agent.
Nano silver using the method for the present invention synthesis is spherical shape, and average diameter is 25.6 nanometers, in crystallization under natural conditions
Shape, purity is very high, and up to 84.4%, chemical property is highly stable, still very stable in 250 degree of high temperature.The invention has weight
The application value wanted.The synthesis technology is quick, economical, environmentally friendly, is free of any toxic chemical.The Nano silver grain of biosynthesis
Tool has been widely used, and especially in timber and timber industry, and may be used as wood protection since it drives termite activity
Agent.
Innovative point of the present invention:
1, in method of the invention, comospore Glochidion puberum is widely distributed, raw material are simple and easy to get；Preparing comospore puberulous glochidion branchlet and leaf
Son plant extraction liquid when, in the prior art the difference is that, process be fresh leaf directly boil, and in the prior art mostly
Number is drying milling, is added after distilled water is heated to reflux and cools down and be centrifuged.
2, the method that the present invention obtains pure reducing agent is filtration method, and the prior art mostly uses centrifuge to be centrifuged.
3, photocatalysis is not needed in nano silver generation step of the invention, can be produced under the conditions of closing is protected from light.
4, the nano-silver ionic that the present invention synthesizes has high stability, and for spherical shape, average diameter is 25.6 nanometers, natural item
It is in crystalloid under part, purity is very high, and up to 84.4%, chemical property is highly stable, still very stable in 250 degree of high temperature.It receives
Rice ag material surface combines the plant source organic chemistry functional group for promoting stability, and 200 DEG C or more chemical bonds just start to be broken.
5, the nano silver particles of biosynthesis of the present invention have effects that repellent termite activity, can be used as Wood protecting agent
Application.
For with the prior art, beneficial effects of the present invention:
1, present invention eliminates baking step directly with clean fresh leaf, the environmental protection of more time-saving energy-saving.
2, for centrifugation technique, filtering method of purification of the invention requires low and saves the energy to mechanical equipment, easily
In scale of mass production.
3, nano silver synthetic environment is light protected environment, it is easier to realize closing production and scale of mass production.
4, the plant source organic chemistry functional group that nano silver material produced by the invention combines can promote the stability of product.
5, the nano silver particles of biosynthesis of the present invention have effects that repellent termite activity, therefore the conduct in timber industry
Wood protecting agent is widely used.
6, for the nano silver particles of biosynthesis of the present invention to plant close friend, seed sprouting is the most fragile in plant life history
Stage, and the nano silver particles of biosynthesis of the present invention to seed sprout nonhazardous effect.
Attached drawing and Detailed description of the invention
Fig. 1 is the nano silver particles transmission electron microscope scanning figure of synthesis；
Fig. 2 is the ultraviolet-visible spectrogram of biosynthesis AgNPs；
Fig. 3 is the AgNPs XRD spectrum figure of biosynthesis；
Fig. 4 is the AgNPs SEM micrograph of biosynthesis；
Fig. 5 is the EDAX ideograph of biosynthesis AgNPs；
Fig. 6 is the FTIR spectrum figure of biosynthesis AgNPs；
Fig. 7 is the thermal stability that TGA detects biosynthesis AgNPs；
Fig. 8 is biosynthesis AgNPs repellent termite experimental result, and Fig. 8 A, Fig. 8 B, Fig. 8 C, Fig. 8 D repeat test result；
Fig. 9 is that Y-shaped smell sensing apparatus detects biosynthesis AgNPs repellent termite experimental result；
Figure 10 is the influence that the nano silver particles of biosynthesis sprout seed, and Figure 10 A, Figure 10 B, Figure 10 C are respectively indicated
The case where Cajanus cajan seeds are sprouted under water, AgNO3 and AgNPs processing；
Figure 11 be pigeonpea germination test three processing young root sectional drawings, Figure 11 A, Figure 11 B, Figure 11 C be illustrated respectively in water,
AgNO3 and AgNPs handles silver ion accumulation in lower young root cell.
Specific embodiment
Be synthetically produced in scheme in the canonical biometric of silver nano-grain according to the present invention, firstly, collect it is fresh, healthy,
Bottle-green comospore Glochidion puberum blade.After transporting to laboratory, leaf is suitably cleaned with distilled water, removal dust is miscellaneous with other
Matter.After washing, dries and be kept in dark place.
1. the biosynthesis of nano silver particles
Synthesis process relates generally to two steps: the i) preparation of leaf extract, ii) it is reacted with AgNO3.
Embodiment 1:
1) fresh, healthy comospore puberulous glochidion branchlet and leaf is acquired, blade is washed with distilled water, to remove dust and other impurities；
It is dried after cleaning spare；
2) fresh leaf that 10 grams are cleaned and dried is cut into small pieces with scissors and is put into sterile glass burning by the preparation of leaf extract
Cup, is added 100 milliliters of distilled water, boils in micro-wave oven 3 minutes, and extracted, and extraction temperature is 10 degree, then mentions leaf
Liquid cooling is taken but to filter afterwards through Whatmann1 filter paper, filtered extracting solution is in 4 DEG C-preservation；
3) fresh nitric acid silver AgNO3, concentration 1mol/L are prepared with sterile distilled water with reacting for AgNO3, in sterile cone
The 100mL AgNO3 prepared is added in shape flask, above-mentioned leaf extract is added, then conical flask is covered with aluminium foil, 2.5 ml
The mixing of fresh leaf extract, beaker is sealed with aluminium-foil paper then, is stood under 30 degree dark conditions until solution is in brown
Color.
Embodiment 2:
Difference from Example 1 be the extraction temperature in step 2) be 100 degree, in step 3) with the fresh leaf of 15ml
Piece extracting solution hybrid reaction, with constant-temperature table with the concussion of 500rpm speed under 20 degree of dark conditions, until solution is in brown.
Embodiment 3:
Difference from Example 1 is that step 3 is with constant-temperature table with the shake of 100rpm speed under 40 DEG C and dark condition
It swings, until solution switchs to brown, indicates the synthesis of nano silver.
Embodiment 4:
Difference from Example 2 is, uses constant-temperature table under 30 DEG C and dark condition with 400rpm speed in step 3
Concussion indicates the synthesis of nano silver until solution switchs to brown.
2. the characterization of biosynthesis nano silver particles
Inventor using X-ray diffraction (XRD), electron microscope, EDAX, FTIR and TGA to the AgNP of biosynthesis into
It has gone the characterization of optics, structure, pattern, element, function and hot property, has seen Fig. 1.UV-vis absorption spectrum method is for evaluating
The synthetic reaction of AgNPs.Fig. 2 shows the ultraviolet spectra of the AgNPs of the biosynthesis of 4 hour records after reaction starts, most
The peak large surface plasma resonance (SPR) is recorded at 457nm.
The crystallographic property of the AgNPs of biosynthesis is confirmed by X-ray diffraction (XRD) analysis.XRD is composed at 2 angles θ
Four characteristic diffraction peaks of (38.10 °, 44.25 °, 64.20 ° and 77.80 °) displays, these characteristic diffraction peaks are respectively belonging to AgNP
(111), (200), (220) and (311) face-centred cubic structure (fcc) Bragg reflection peak (Fig. 3).
For Morphological Characterization, scans (SEM) and transmission (TEM) electron microscope carries out.SEM and TEM microphoto is shown
The spherical morphology of the AgNPs of biosynthesis, average-size are about 25.6 nanometers (Fig. 4)
The element composition (Fig. 5) of the AgNPs of biosynthesis is determined with EDAX analysis.Strong peak at 3kev shows silver
(Ag) be biosynthesis AgNPs in essential element, show the purity of sample.And other peaks (carbon and oxygen) mention corresponding to leaf
Take biomolecule present in object.
Analyze functional group relevant to the AgNPs of synthesis using FTIR (in plant biological molecule).These are planted
Object biomolecule actually plays important work in the further stabilization of the biological reducing of silver ion and the AgNPs of biosynthesis
With.Infrared spectroscopy shows stronger absorption spectra at 3434,2917,1624,1443,1385,1207,1027 and 514cm-1
Band.The O-H stretching vibration that peak at 3434cm-1 is generated corresponding to the presence due to pure and mild phenol.Another peak exists
Alkane/aromatic compound C-H group is assigned at 2917cm-1.Band at 1624,1443cm-1, which corresponds to, to be existed
C-N, C-C and N-H stretching vibration in protein.Band in 1385cm~1 is produced since the N-O of nitro compound is flexible
It is raw.Peak near 1207 and 1027cm-1 illustrates that aliphatic C-N and carbonyl in protein extend.Band at 514cm-1 is real
It is the stretching vibration due to alkynes on border and generates (referring to Fig. 6).
Thermal stability investigation is carried out using AgNPs of the thermogravimetric analysis (TGA) to biosynthesis.In 25~700 DEG C of temperature
AgNPs sample is heated in range.At a temperature of 100~200 DEG C, the minimum weight-loss ratio of the first step is 5.02%, this
Caused by the moisture that weightlessness is practically due to be adsorbed onto the surface AgNPs is removed.200~700 DEG C at a temperature of, observe bright
Aobvious second step is weightless (15.52%), and this weightlessness is due to caused by the biomolecule desorption for being present in the surface AgNPs.Most
Importantly, sample is heated to obtain 82.85% residual weight after 700 DEG C, it is aobvious to show that the AgNPs of biosynthesis has
The thermal stability (such as Fig. 7) of work.
3. the expulsion termite activity of biosynthesis AgNPs is analyzed
Termite is acquired from the woods in forest by infestation, termite is lightly picked up and is transported to laboratory.
It is measured with trace paper technology, filter paper disk is divided into two equal parts.Half part is soaked in water, the other half immersion
In 1 milliliter of AgNPS suspension.The two parts for being impregnated with blotting paper all put the distance for keeping big between the two parts.Then will
The termite colony of 10~15 collections is equably put into filter paper disk.The movement of termite is monitored, to understand the whereabouts of termite.24
After hour, it has been found that the maximum population of termite resides on the suction strainer paper being soaked in water (referring to Fig. 8).Repeatedly test,
Observe the characteristics of motion of termite.
Another experiment smells instrument using Y pipe to detect the activity of biosynthesis AgNPs expulsion termite.This experiment will impregnate
The small pieces scraps of paper in water and AgNPs suspension are respectively placed in the left and right arms of Y pipe olfactometer, and 20 termites are in the lower arm of Y pipe
Release.After release, sealed immediately with tampon.After carrying out 24 hours at room temperature, the termite in left and right arms is counted, to check
The repellent activity to termite of AgNPs.The arm end of soaking water it can be seen in figure 9 that most of termite crawls toward.
The influence that seed is sprouted in 4.AgNPs processing
In order to study whether the AgNPs of biosynthesis has toxic effect to plant germination, carried out in vitro
Seed Germination Test.Cajanus cajan seeds are suitably cleaned with water, with blotting paper dry tack free.Control group (water) (Figure 10 A), silver nitrate
(50 μ g/ml) (Figure 10 B), three groups of AgNPs (50 μ g/ml) (Figure 10 C).Seed impregnates 1 hour in respective suspension.?
After this, seed is put into the PET sheet containing moisture absorption ink paper, and is placed at room temperature.As shown in Figure 10, control group water (figure
10A) and the seed of the AgNPs of biosynthesis (Figure 10 C) processing is sprouted well, and AgNO3 is inhibited to seed sprouting.
Another group of identical experiment is monitored by plant living body imaging system to observe the process of sprouting.We have found that AgNO3 from Figure 11
The cross section in processing radicle area shows the phenomenon that silver in area of vascular bundles accumulates, this may be their inhibition to germination
Property influence possible cause.And the size of the AgNPs of the biosynthesis in the present invention is about 25.6nm, the nano particle is to thin
The influence very little of born of the same parents.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all in spirit of the invention and
Any modification, equivalent replacement for being made within principle etc., should all be included in the protection scope of the present invention.
Claims (7)
1. a kind of method with comospore Glochidion puberum blade biosynthesis nano silver particles, comprising the following steps:
1) fresh, healthy comospore puberulous glochidion branchlet and leaf is acquired, blade is washed with distilled water, to remove dust and other impurities；It cleans
After dry it is spare；
2) fresh leaf that 10 grams are cleaned and dried is cut into small pieces with scissors and is put into sterile glass beaker, added by the preparation of leaf extract
Enter 100 milliliters of distilled water, heat and extracted, then will be filtered after leaf extract cooling through Whatmann1 filter paper, filtering
Extracting solution afterwards is in 4 DEG C-preservation；
3) fresh nitric acid silver AgNO3, concentration 1mol/L are prepared with sterile distilled water with reacting for AgNO3, is burnt in sterile taper
The 100mL AgNO3 prepared is added in bottle, above-mentioned leaf extract is added, then conical flask is covered with aluminium foil, is placed on dark
Under the conditions of, standing or the whirling vibration in rotor-vibrating screen, until solution becomes brown.
2. the method according to claim 1 with comospore Glochidion puberum blade biosynthesis nano silver particles, it is characterised in that:
Extraction temperature range described in step 2 is 10-100 DEG C.
3. the method according to claim 1 with comospore Glochidion puberum blade biosynthesis rice silver particles, described in step 3
The silver nitrate of every 100ml concentration 1mM can carry out reacting nitric acid synthesis silver with 2.5-15ml leaf extract.
4. the method according to claim 1 with comospore Glochidion puberum blade biosynthesis nano silver particles, it is characterised in that:
Synthesis reaction temperature described in step 3 is 20-40 DEG C.
5. the method according to claim 1 with comospore Glochidion puberum blade biosynthesis nano silver particles, it is characterised in that:
The oscillation frequency range of rotor-vibrating screen described in step 3 is 100-500rpm.
6. according to claim 1 use comospore Glochidion puberum blade biosynthesis nano silver, which is characterized in that sodium rice silver is ball
Shape, average diameter are 25.6 nanometers, are in crystalloid under natural conditions.
7. the nano silver particles of the synthesis of method described in -5 any claims are used as answering for Wood protecting agent according to claim 1
With.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111097921A (en) * | 2020-01-13 | 2020-05-05 | 山西大学 | Anti-colon cancer silver nanoparticles and preparation method thereof |
| CN111515384A (en) * | 2020-04-10 | 2020-08-11 | 深圳大学 | Nano-silver material mediated and synthesized by moringa seed extract |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103302306A (en) * | 2013-06-19 | 2013-09-18 | 东南大学 | Method for reducing and preparing functionalized nano-silver based on polyphenol |
| CN103949658A (en) * | 2014-05-09 | 2014-07-30 | 武汉纺织大学 | Green synthesis method of nano-silver by utilizing eucommia ulmoides water extract |
| CN104889419A (en) * | 2015-05-18 | 2015-09-09 | 湖州品创孵化器有限公司 | Biological synthesis method for spherical silver nanoparticle |
| CN104889418A (en) * | 2015-05-18 | 2015-09-09 | 湖州品创孵化器有限公司 | Biological synthesis method of cubic nanometer silver particles |
| CN104923805A (en) * | 2015-07-03 | 2015-09-23 | 渤海大学 | Micro-nano-silver base material preparation method and micro-nano-silver base material thereof |
| CN105642911A (en) * | 2016-01-27 | 2016-06-08 | 陕西科技大学 | Synthesizing method for nanometer Ag |
| CN105665737A (en) * | 2016-01-27 | 2016-06-15 | 陕西科技大学 | Biosynthesis method of nano Ag |
| KR101646617B1 (en) * | 2015-02-17 | 2016-08-09 | 부산대학교 산학협력단 | Preparing method of silver nanoparticles using phytochemicals-derived organic acid |
| CN106513707A (en) * | 2016-12-07 | 2017-03-22 | 鲁东大学 | Nano silver bacteriostatic agent biosynthesized through blueberry leaf extracting solution and preparation process of nano silver bacteriostatic agent |
| MX2016015286A (en) * | 2016-11-04 | 2018-05-03 | Maria Guajardo Pacheco Jesus | Wet reduction process by the electrochemical method to produce metallic nanocolloids, metallic oxides, or the deposition of metallic nanoparticles on the surface of fine particles. |
-
2019
- 2019-04-26 CN CN201910344232.1A patent/CN109909513B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103302306A (en) * | 2013-06-19 | 2013-09-18 | 东南大学 | Method for reducing and preparing functionalized nano-silver based on polyphenol |
| CN103949658A (en) * | 2014-05-09 | 2014-07-30 | 武汉纺织大学 | Green synthesis method of nano-silver by utilizing eucommia ulmoides water extract |
| KR101646617B1 (en) * | 2015-02-17 | 2016-08-09 | 부산대학교 산학협력단 | Preparing method of silver nanoparticles using phytochemicals-derived organic acid |
| CN104889419A (en) * | 2015-05-18 | 2015-09-09 | 湖州品创孵化器有限公司 | Biological synthesis method for spherical silver nanoparticle |
| CN104889418A (en) * | 2015-05-18 | 2015-09-09 | 湖州品创孵化器有限公司 | Biological synthesis method of cubic nanometer silver particles |
| CN104923805A (en) * | 2015-07-03 | 2015-09-23 | 渤海大学 | Micro-nano-silver base material preparation method and micro-nano-silver base material thereof |
| CN105642911A (en) * | 2016-01-27 | 2016-06-08 | 陕西科技大学 | Synthesizing method for nanometer Ag |
| CN105665737A (en) * | 2016-01-27 | 2016-06-15 | 陕西科技大学 | Biosynthesis method of nano Ag |
| MX2016015286A (en) * | 2016-11-04 | 2018-05-03 | Maria Guajardo Pacheco Jesus | Wet reduction process by the electrochemical method to produce metallic nanocolloids, metallic oxides, or the deposition of metallic nanoparticles on the surface of fine particles. |
| CN106513707A (en) * | 2016-12-07 | 2017-03-22 | 鲁东大学 | Nano silver bacteriostatic agent biosynthesized through blueberry leaf extracting solution and preparation process of nano silver bacteriostatic agent |
Non-Patent Citations (1)
| Title |
|---|
| 李鹏等: "《贵金属材料与工艺》", 31 August 2015, 武汉：中国地质大学出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111097921A (en) * | 2020-01-13 | 2020-05-05 | 山西大学 | Anti-colon cancer silver nanoparticles and preparation method thereof |
| CN111097921B (en) * | 2020-01-13 | 2021-05-14 | 山西大学 | Anti-colon cancer silver nanoparticles and preparation method thereof |
| CN111515384A (en) * | 2020-04-10 | 2020-08-11 | 深圳大学 | Nano-silver material mediated and synthesized by moringa seed extract |
| CN111515384B (en) * | 2020-04-10 | 2022-05-24 | 深圳大学 | Nano-silver material mediated and synthesized by moringa seed extract |
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