CN109892340A - Application of the bacillus alvei in prevention and treatment potato scab - Google Patents
Application of the bacillus alvei in prevention and treatment potato scab Download PDFInfo
- Publication number
- CN109892340A CN109892340A CN201910148992.5A CN201910148992A CN109892340A CN 109892340 A CN109892340 A CN 109892340A CN 201910148992 A CN201910148992 A CN 201910148992A CN 109892340 A CN109892340 A CN 109892340A
- Authority
- CN
- China
- Prior art keywords
- potato
- scab
- potato scab
- bacillus
- streptomycete
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 244000061456 Solanum tuberosum Species 0.000 title claims abstract description 57
- 235000002595 Solanum tuberosum Nutrition 0.000 title claims abstract description 56
- 206010039509 Scab Diseases 0.000 title claims abstract description 43
- 241000178961 Paenibacillus alvei Species 0.000 title claims description 20
- 230000002265 prevention Effects 0.000 title description 10
- 241001655322 Streptomycetales Species 0.000 claims abstract description 13
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 9
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 7
- 241000187181 Streptomyces scabiei Species 0.000 claims description 14
- 241000142909 Streptomyces acidiscabies Species 0.000 claims description 11
- 241000187747 Streptomyces Species 0.000 claims description 5
- 230000001580 bacterial effect Effects 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 2
- 239000007921 spray Substances 0.000 claims description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 23
- 201000010099 disease Diseases 0.000 abstract description 21
- 241000196324 Embryophyta Species 0.000 abstract description 9
- 244000005700 microbiome Species 0.000 abstract description 5
- 238000011161 development Methods 0.000 abstract description 3
- 239000012681 biocontrol agent Substances 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 description 27
- 238000012545 processing Methods 0.000 description 9
- 239000013642 negative control Substances 0.000 description 8
- TWFZGCMQGLPBSX-UHFFFAOYSA-N carbendazim Chemical compound C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 230000002401 inhibitory effect Effects 0.000 description 5
- 230000003902 lesion Effects 0.000 description 5
- 230000001717 pathogenic effect Effects 0.000 description 5
- 230000003042 antagnostic effect Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 241000233866 Fungi Species 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000000443 biocontrol Effects 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- 238000002791 soaking Methods 0.000 description 3
- 239000003053 toxin Substances 0.000 description 3
- 231100000765 toxin Toxicity 0.000 description 3
- 244000063299 Bacillus subtilis Species 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000006013 carbendazim Substances 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 235000012015 potatoes Nutrition 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000223221 Fusarium oxysporum Species 0.000 description 1
- 241001646398 Pseudomonas chlororaphis Species 0.000 description 1
- 206010065954 Stubbornness Diseases 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000843 anti-fungal effect Effects 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000002969 morbid Effects 0.000 description 1
- 230000024241 parasitism Effects 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 238000009666 routine test Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 239000004563 wettable powder Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
Abstract
The invention discloses honeycomb series bacillus to prepare the application in the microbial inoculum for preventing and treating potato scab, belongs to the development and utilization field of microorganism germ plasma resource.The present invention provides the new application of honeycomb series bacillus, can efficiently inhibit the growth of scab streptomycete using biocontrol agent prepared by honeycomb series bacillus, can be used for preventing and treating by scab streptomycete (StreptomycesThe plant diseases such as potato scab caused by spp.), have a extensive future.
Description
Technical field
The invention belongs to the development and utilization fields of microorganism germ plasma resource more particularly to bacillus alvei in prevention and treatment horse
Application in bell potato shot hole.
Background technique
Potato is third place in the world generalized grain crop.However, as four major disease of potato (shot hole, late blight, powder scab
Disease and virosis) one of potato scab but annoying potatoes production always, especially to being cultivated in greenhouse
Virus-free seed potato causes to seriously threaten, production loss 20% or so, since Streptomyces scabies generates toxin, to Potato Quality
Influence it is even more serious.During toxicity micro potato is mass produced, shot hole incidence is up to 60% or more.In recent years, should
Disease occurs rampant in southern area.The area crop field spring sowing potato of Jinhua, Zhejiang Province in 2018 harvests season, potato tubers shot hole
Aggrieved serious (Fig. 1), the disease incidence of part field have reached 90% or more.Recently, in the potato sore in some places in Yunnan Province
Scall incidence up to 100%, has seriously affected potato yield and quality, becomes the main bottle for restricting potatoes production
One of neck.
Separation qualification result is carried out to the pathogenic bacteria for potato tubers of falling ill and shows it is by pathogenic scab streptomycete
(Streptomyces spp.) causes, and toxin is the pathogenic Main Factors of Streptomyces scabies.It is ground through Zhejiang University's potato scab
Study carefully seminar's identification, the advantage potato scab kind in Zhejiang Province is Streptomyces scabies and Streptomyces
Acidiscabie, they generate shot hole toxin.
It can be effectively prevented from disadvantages mentioned above using biological control means prevention and treatment crop disease, thus with before wide development
On the way.Wherein antagonistic bacterium occupies important one as a kind of important biological control agent in the integrated control of plant disease
The ground of seat.
Main advantage of the bacterium in biological control application is: (1) type and quantity of bacterium are numerous, in plant rhizosphere
Largely exist with overground part；(2) bacterium is wider to the mode of action of pathogen, can be planted by competition, antagonism and parasitism, induction
Object generates the modes such as resistance and has an impact to pathogen；(3) there is surprising reproduction speed；(4) many bacteriums are present in plant
Rhizosphere and overground part, the ecology comparison to plant are suitable for；(5) bacterium can manually be cultivated mostly, convenient for control, in practice easily
In operation；(6) some bacteriums can not only controlling disease and also have growth-promoting functions, crop yield can be increased.
Cause potato scab scab streptomycete be a kind of stubbornness soil inhabitant, can pass through soil-borne disease
Evil seriously affects the yield and quality of potato tubers once morbidity is difficult to control.It is well known that plantation disease-resistant variety is anti-
It cures the disease harmful most efficient method, but so far, there is no the kind of anti-potato scab both at home and abroad, this may be with the cause of germ
Sick characteristic has substantial connection.The cultural control means of disease also can not effectively carry out because being limited by region and all many conditions.Mesh
It is preceding prevention and treatment potato scab main means be using chemical bactericide, in chemical prevention, agricultural streptomycin and copper agent
It has become " the omnipotent medicament " of prevention and treatment bacteriosis, however largely causes the drug resistance of germ to enhance using pesticide due to long-term,
" the omnipotent medicament " of both prevention and treatment bacteriosises is substantially invalid to prevention and treatment potato scab.Natural ecological environment pollution and
The problem of pesticide residue, is more and more prominent, and the new way for seeking crop disease control is always the target of people's effort.
Summary of the invention
In order to solve the above-mentioned technical problem, inventor is surprised to find that bacillus alvei can effectively inhibit streptomycete
Growth, to prevent and treat potato scab, thereby completing the present invention.
An aspect of of the present present invention provides bacillus alvei and is preparing answering in the microbial inoculum for preventing and treating potato scab
With.
In one embodiment of the invention, the bacillus alvei is that deposit number is CCTCC M
2012448 bacillus alvei bacterial strain ZJUB2011-1.
In embodiments of the invention, the microbial inoculum is powder preparation, liquid preparation or solid pharmaceutical preparation.
In specific embodiments of the present invention, the microbial inoculum can by spray or the method for pouring root apply.
In other specific embodiments of the invention, the concentration of honeycomb series bacillus is 10 in the microbial inoculum7~
108cfu/mL。
In embodiments of the invention, the potato scab is drawn by streptomycete (Streptomyces.spp)
It rises.
In some specific embodiments of the invention, the streptomycete is selected from S.scabies and S. acidiscabie
At least one of, i.e., the described potato scab is separately or cooperatively caused by S.scabies and S.acidiscabie.
In one embodiment of the invention, the streptomycete is S.scabies and S.acidiscabie.I.e.
The potato scab is caused jointly by S.scabies and S.acidiscabie.
Beneficial effects of the present invention
Compared with prior art, the invention has the benefit that
Honeycomb series bacillus ZJUB2011-1 can efficiently inhibit the life of scab streptomycete (Streptomyces spp.)
It is long, it can be used for preventing and treating and the plant diseases such as caused potato scab infected by the germ, have a extensive future.
Detailed description of the invention
Fig. 1 shows the aggrieved situation of potato tubers shot hole.
Fig. 2 shows living body protection effects (to be followed successively by blank control, bacillus alvei ZJUB2011-1 processing；It is negative
Control and the processing of positive control carbendazim).
Specific embodiment
In order to which the technical problems, technical solutions and beneficial effects solved by the present invention is more clearly understood, below in conjunction with
Embodiment, the present invention will be described in further detail.
Embodiment
Following example is used herein to demonstration the preferred embodiments of the invention.Those skilled in the art, it will be appreciated that under
State the technology disclosed in example represent inventor discovery can be used for implementing technology of the invention, therefore can be considered as implementation this
The preferred embodiment of invention.But those skilled in the art should be understood that specific reality disclosed herein according to this specification
Many modifications can be made by applying example, still can be obtained identical or similar as a result, rather than away from the spirit or scope of the present invention.
Unless otherwise defined, the term of all technologies as used herein and science, and the technology in fields of the present invention
Personnel institute is normally understood equivalent in meaning, and being disclosed reference and their materials of reference will all be incorporated.
Those skilled in the art will recognize or just will appreciate that by routine test many described here
Invention specific embodiment many equivalent technologies.These will equally be comprised in claims.
1 potato scab disease fungus of embodiment is isolated and purified and is identified
Natually morbid potato scab potato wedge is acquired from Academy of Agricultural Sciences, Jinhua, Zhejiang Province city Experimental Base.Foundation
The potato scab pathogenic bacteria of acquisition are inoculated into healthy potato potato wedge by Koch rule, after potato scab occurs
Separate again, isolate and purify to obtain more pathogen strain bacterium from potato scab potato wedge, through with potato scab standard control bacterium
Strain comparison and molecular biology identification, germ be accredited as 2 kinds of pathogenic bacteria Streptomyces scabies and
Streptomyces acidiscabie, 2 plants of final choice representational 2016001 Hes of pathogenic bacteria S.scabies
S.acidiscabie 2016016 uses bacterium as test inoculation.
2 antagonistic bacterium of embodiment
It is instruction with above-mentioned 2 plants representative pathogenic bacteria S.scabies 2016001 and S.acidiscabie 2016016
Bacterium, using tablet face-off method, screening is to potato scab bacterium in the pure culture bacterium single colonie that this laboratory had previously obtained
Inhibited bacterial strain.
Potato scab bacterium mycelia block (diameter about 7mm) is placed in PDA plate center, point connects in plate edge away from center
At 25mm, each culture dish connects 4 points, 28 DEG C of culture 3d, and obtaining one plant has inhibitory effect bacterial strain to 2 kinds of potato scab bacterium.
The bacterial strain is Paenibacillus alvei ZJUB2011-1, is saved in Chinese Typical Representative on November 6th, 2012
Culture Center (CCTCC), deposit number are CCTCC NO:M 2012448, preservation address: China, Wuhan, Wuhan University.
The measurement of 3 biocontrol microorganisms Antifungal Activity in Vitro of embodiment
It is beaten with the punch that punching diameter is 7mm and takes eugonic Fusarium oxysporum bacteria cake, transferred in PDA plate
Centre, with point meets the resulting bacillus alvei ZJUB2011-1 of screening respectively on its four angle at a distance of 2cm, by culture dish
It is placed in 28 DEG C of insulating boxs and cultivates 6d, with bacillus subtilis (Bacillus subtilis) biocontrol microorganisms 1 for Positive contrast bacteria,
Not connecing Antagonistic Fungi is negative control, and each processing is repeated 3 times.
It is for statistical analysis to each group result using DPS software.Analysis is the results show that bacillus alvei ZJUB2011-
1 pair of potato scab bacterium has more significant rejection ability, and inhibiting rate reaches 71.00%, is higher than Positive contrast bacteria.Experiment knot
Fruit is as shown in table 1 below.
The in vitro inhibition of potato Streptomyces scabies effect of 1 bacillus alvei ZJUB2011-1 of table
| Processing | Colony diameter (mm) | Inhibiting rate (%) |
| Bacillus alvei ZJUB2011-1 | 26.3±0.85c | 71.00 |
| Bacillus 1 | 81.6±0.87a | 0 |
| Antagonistic Fungi is not connect | 83.2±0.78a | 0 |
Note: digital upper right corner letter indicates the analysis of Deng Kenshi multiplexed differential as a result, same column difference same word matrix in 1. tables
Show significant difference (P < 0.05)；
2. inhibiting rate (%)=100 × (negative control colony diameter-processing colony diameter)/(negative control bacterium colony is straight
Diameter -7mm)
The measurement of 4 biocontrol microorganisms living body protection effect of embodiment
It is 1 × 10 that concentration, which is made, in bacillus alvei ZJUB2011-1-1 by processing group8The bacteria suspension of CFU/mL chooses nothing
Disease pest harm is immersed in bacteria suspension, soaking time 30min without wound, maturity and the almost the same potato tubers of size,
Potato scab bacterium S. scabies 2016001 and S.acidiscabie is inoculated on potato tubers after time 12h
The Mixed Microbes (1:1) of 2016016 bacterium simulate the germ composition in natural occurrence.
Three control groups: 50% carbendazol wettable powder of conventional pesticide (Jiangsu Lan Feng biochemical industry share are set simultaneously
Co., Ltd) 300 times of dilutions seed soaking after inoculation potato scab bacterium be positive control；Horse is inoculated with after soaking seed with sterile water
Bell potato Streptomyces scabies is negative control；Potato scab bacterium is not connect as blank control to be inoculated with sterile water.Set 28 DEG C of culture
It is cultivated in case, investigates incidence after 10d.3 repetitions are arranged in each processing, are averaged.As a result as shown in Fig. 2, table 2.
Living body control efficiency of each treatment fluid of table 2 to potato scab
| Processing | Disease index | Control efficiency (%) |
| Bacillus alvei ZJUB2011-1 | 38.00a | 61.12 |
| 50% carbendazim (positive control) | 93.33b | 0 |
| Negative control | 93.33b | 0 |
| Blank control | 0 | 0 |
The severity Scaling standard of potato scab are as follows:
(1) 0 grade:: it is asymptomatic；
(2) 1 grades: lesion area accounts for the 0.1~1/5 of entire stem tuber；
(3) 2 grades: lesion area accounts for the 1/5~2/5 of entire stem tuber；
(4) 3 grades: lesion area accounts for the 2/5~3/5 of entire stem tuber；
(5) 4 grades: lesion area accounts for the 3/5~4/5 of entire stem tuber；
(6) 5 grades: lesion area accounts for 4/5 or more of entire stem tuber.
Disease index=(∑ sick stem tuber number × state of an illness class values at different levels)/(investigation sum × highest level value) × 100；It is anti-
Control effect (%)=(negative control disease index-processing disease index)/negative control disease index × 100%.
In conclusion bacillus alvei (Paenibacillus alvei) ZJUB2011-1 is to causing potato scab
The scab streptomycete (Streptomyces spp.) of disease has stronger inhibiting effect, and in living body diseases prevention test, bacterium is outstanding
Liquid has good control efficiency to potato scab, has good production application prospect.
All references mentioned in the present invention is incorporated herein by reference, independent just as each document
It is incorporated as with reference to such.In addition, it should also be understood that, after reading the above teachings of the present invention, those skilled in the art can
To make various changes or modifications to the present invention, such equivalent forms equally fall within model defined by the application the appended claims
It encloses.
Claims (8)
1. bacillus alvei is preparing the application in the microbial inoculum for preventing and treating potato scab.
2. application according to claim 1, which is characterized in that the bacillus alvei is that deposit number is CCTCC M
2012448 bacillus alvei bacterial strain ZJUB2011-1.
3. application according to claim 1 or 2, which is characterized in that the microbial inoculum is powder preparation, liquid preparation or solid
Preparation.
4. application according to claim 3, which is characterized in that the microbial inoculum can by spray or the method for pouring root apply.
5. application according to claim 3 or 4, which is characterized in that the concentration of honeycomb series bacillus is in the microbial inoculum
107~108cfu/mL。
6. application according to claim 1 or 2, which is characterized in that the potato scab is by streptomycete
(Streptomyces.spp) caused by.
7. application according to claim 6, which is characterized in that the streptomycete be selected from S.scabies and
At least one of S.acidiscabie.
8. application according to claim 7, which is characterized in that the streptomycete be S.scabies and
S.acidiscabie。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910148992.5A CN109892340B (en) | 2019-02-28 | 2019-02-28 | Application of paenibacillus nidae in preventing and treating potato scab |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910148992.5A CN109892340B (en) | 2019-02-28 | 2019-02-28 | Application of paenibacillus nidae in preventing and treating potato scab |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109892340A true CN109892340A (en) | 2019-06-18 |
| CN109892340B CN109892340B (en) | 2024-03-08 |
Family
ID=66945897
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910148992.5A Active CN109892340B (en) | 2019-02-28 | 2019-02-28 | Application of paenibacillus nidae in preventing and treating potato scab |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109892340B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103205372A (en) * | 2012-12-11 | 2013-07-17 | 浙江大学 | Paenibacillus alvei and its applications |
| WO2015114552A1 (en) * | 2014-01-29 | 2015-08-06 | University Of Pretoria | Plant growth promoting rhizobacterial strains and their uses |
| CN107432286A (en) * | 2016-05-25 | 2017-12-05 | 中国科学院微生物研究所 | A kind of microbial bacterial agent and its preparation method and application |
-
2019
- 2019-02-28 CN CN201910148992.5A patent/CN109892340B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103205372A (en) * | 2012-12-11 | 2013-07-17 | 浙江大学 | Paenibacillus alvei and its applications |
| WO2015114552A1 (en) * | 2014-01-29 | 2015-08-06 | University Of Pretoria | Plant growth promoting rhizobacterial strains and their uses |
| CN107432286A (en) * | 2016-05-25 | 2017-12-05 | 中国科学院微生物研究所 | A kind of microbial bacterial agent and its preparation method and application |
Non-Patent Citations (1)
| Title |
|---|
| 张彤彤 等，: "6种杀菌剂对马铃薯疮痂病及粉痂病的防效", 《甘肃农业科技》, no. 2, pages 23 - 27 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109892340B (en) | 2024-03-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2705286C2 (en) | Mycosphaerella sp. strain, compositions and methods for fusariosis control | |
| CN101550402B (en) | Bacillus subtilis 203 and applications thereof | |
| Muhammad et al. | In-vitro inhibition of growth of some seedling blight inducing pathogens by compost-inhabiting microbes | |
| Hossain et al. | The plant growth-promoting fungus Penicillium spp. GP15-1 enhances growth and confers protection against damping-off and anthracnose in the cucumber | |
| Abeysinghe | Biological control of Fusarium solani f. sp. phaseoli the causal agent of root rot of bean using Bacillus subtilis CA32 and Trichoderma harzianum RU01 | |
| Ali et al. | Evaluating the efficacy of'Trichoderma'spp and'Bacillus substilis' as biocontrol agents against'Magnaporthe grisea'in rice | |
| CN102010825B (en) | Microorganism compound bactericide for controlling cucumber fusarium wilt and preparation method thereof | |
| Juber et al. | Fusarium wilt disease of strawbrry caused by Fusarium oxysporum f. sp. fragariae in Iraq and its control. | |
| CN104673705B (en) | One plant prevent and treat wheat scab bacillus amyloliquefaciens and its application | |
| Abdel-Monaim et al. | Enhancement of growth parameters and yield components in eggplant using antagonism of Trichoderma spp. against Fusarium wilt disease | |
| Ali et al. | Evaluating the efficacy of Trichoderma isolates and Bacillus subtilis as biological control agents against Rhizoctonia solani | |
| CN109022320A (en) | Siam bacillus and its application in root rot and southern blight prevention and treatment | |
| Shen et al. | The siderophore-producing bacterium, Bacillus siamensis Gxun-6, has an antifungal activity against Fusarium oxysporum and promotes the growth of banana | |
| CN107937312A (en) | Application of one plant of Bei Laisi bacillus in cucumber blight disease | |
| CN114891670A (en) | Bacillus siamensis ZJh with broad-spectrum antibacterial activity and application thereof | |
| CN105154339A (en) | Trichoderma viride strain and application thereof | |
| CN114907986A (en) | Trichoderma harzianum and application thereof in preparation for preventing and treating root rot of panax notoginseng | |
| CN101440356B (en) | Bacillus licheniformis 202 and use thereof | |
| CN108559718A (en) | The myxococcus stipitatus of one plant of predacious plant pathogenetic bacteria and its application in bacterial disease biological control | |
| CN116716225A (en) | Radiation-resistant streptomycete and application thereof | |
| Kouadri et al. | First report of using Trichoderma longibrachiatum as a biocontrol agent against Macrophomina pseudophaseolina causing charcoal rot disease of lentil in Algeria | |
| CN112322515A (en) | Novel strain of Bacillus belgii and application thereof | |
| CN109892340A (en) | Application of the bacillus alvei in prevention and treatment potato scab | |
| Domracheva et al. | Anti-Fusarium activity of cyanobacteria and actinomycetes in soil and rhizosphere | |
| CN114806892A (en) | Trichoderma atroviride strain and application thereof in preventing and treating panax notoginseng root rot |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |