CN109892327A - A kind of preparation method and applications of microorganism seed coating - Google Patents
A kind of preparation method and applications of microorganism seed coating Download PDFInfo
- Publication number
- CN109892327A CN109892327A CN201910299546.4A CN201910299546A CN109892327A CN 109892327 A CN109892327 A CN 109892327A CN 201910299546 A CN201910299546 A CN 201910299546A CN 109892327 A CN109892327 A CN 109892327A
- Authority
- CN
- China
- Prior art keywords
- trichoderma
- preparation
- fermentation liquid
- seed coating
- agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The present invention discloses a kind of preparation method and applications of microorganism seed coating, belongs to agricultural biological technical field, comprising the following steps: (1) provide the fermentation liquid of trichoderma chlamydospore；The trichoderma includes trichoderma harzianum and trichoderma viride；(2) fermentation liquid and dispersing agent, film forming agent and 5% protective agent containing trichoderma chlamydospore are mixed；(3) after the completion of step (2) are stirred, binder, seed is added；(4) cold water of remaining protective agent quality 50% is added in remaining protective agent, stirs evenly, is heated to 60 DEG C of lasting 2min to uniform liquid is formed, cooling is spare；(5) protective agent of the fermentation liquid and step (4) that obtain step (3) is uniformly mixed, and mixed liquor is spray-dried to obtain the final product；The present invention helps to solve the problems, such as that the Trichoderma spore holding time is short, is unable to large-scale production using chlamydospore as the effective component of preparation, is able to satisfy the requirement as biological pesticide.
Description
Technical field
The present invention relates to agricultural biological technical field, preparation method more particularly to a kind of coating of microorganism seed and its
Using.
Background technique
Coating agent for seed, which refers to, is process by related agents, nutrient, film forming agent, dispersing agent and auxiliary agent and is formed tool
Have some strength and permeability and it can directly be coated on the active suspending agent of the surface of the seed.
Coating agent for seed is a kind of special preparation for integrating the performances such as health care, nutrition, protection, degeneration-resistant, has seed
Disinfection, slow release medicine fertilizer, avoids injuring natural enemy, reducing environmental pollution, improve crop anti-adversity etc. making the prevention and treatment disease pest plague of rats
With.
Currently, in the related technology, the ingredient of common coating agent for seed is mainly active component and non-active ingredient；Wherein
Active constituent has insecticide, fungicide, growth regulator, nutriment and microorganism etc..Inactive ingredients have filler material, moisten
Agent, oxidant, film forming agent, suspending agent, colloid protective agent, viscosity stabiliser, diffusant, antifreezing agent, acid-base modifier, anti-corrosion
Agent, defoaming agent, Security alert color etc..
In the 1930s, Weindling discovery Trichoderma is able to suppress the growth of soil-borne disease fungal pathogens.From 20th century 70
Since age, the biological control mechanism of Trichoderma is widely studied, and discovery Trichoderma has significant Biocontrol Effect and growth promotion
Effect.The biocontrol mechanism of Trichoderma mainly includes antagonism, hyperparasitism, the enzyme for secreting dissolution cell wall.Trichoderma
Antagonism range has broad spectrum activity, and correlative study shows that Trichoderma at least has antagonism to 18 categories, 29 kinds of disease fungus.
Trichoderma is mainly used in the inhibition that chemical pesticide is difficult to the soil-borne pathogen to play a role at present, especially to botrytis cinerea
(Botrytiscinerea), Rhizoctonia (Rhizotonia), Fusarium (Fusarium), Phytophthora
(Phytophthora) etc. disease fungus have preferable control efficiency.
However, seed pelleting negligible amounts based on trichoderma currently on the market, and Trichoderma coating effective component is
Conidium, such as the trichoderma harzianum of Bai Wo company, the U.S., doctor Niu of Shandong Tylenol medicine company.All there is spore storage in these preparations
Deposit that unstable and shelf life is short, the time that is stored at room temperature of viable bacteria does not exceed 3 months, and the trichoderma survival volume in preparation is difficult to
Reach requirement, the stability of preventive effect cannot be effectively ensured, simultaneously as conidium is solid state fermentation acquisition, it is difficult to
Large-scale production, so the trichoderma bacteria preparation of market sale at present still cannot substituted chemistry agriculture completely in control of plant disease
Medicine.In terms of being currently concentrated mainly on its Production conditions and shaking flask culture optimization to the research of trichoderma chlamydospore both at home and abroad, for
Trichoderma harzianum chlamydospore industrializes liquid fermentation and the research of preparation is seldom.
Summary of the invention
To solve the above problems, the present invention provides following technical schemes.
On the one hand, the present invention provides a kind of preparation method of microorganism seed coating, comprising the following steps:
(1) fermentation liquid of trichoderma chlamydospore is provided；The trichoderma includes trichoderma harzianum (Trichodermaviride)
With trichoderma viride (Trichoderma viride)；
(2) fermentation liquid containing trichoderma chlamydospore is mixed with the protective agent of dispersing agent, film forming agent and 5% mass, 600r/
Min is stirred 10-20 minutes；
(3) after the completion of step (2) are stirred, binder, seed is added, the mass ratio of binder and seed is 50:9,
600r/min stirs 5min；
(4) cold water of remaining protective agent quality 50% is added in remaining protective agent, stirs evenly, is heated to 60 DEG C and continues
For 2min to uniform liquid is formed, cooling is spare；
(5) protective agent of the fermentation liquid and step (4) that obtain step (3) is uniformly mixed, and mixed liquor is spray-dried,
Microorganism seed coating is made；
Wherein, the content of the binder is the 8%-25% (kg/L) of fermentation liquid, and protectant content is fermentation
The 60%-80% (kg/L) of liquid, the content of the dispersing agent are the 4-10% (kg/L) of fermentation liquid, and the content of the film forming agent is
The 2-5% (kg/L) of fermentation liquid；The content of trichoderma chlamydospore described in the microorganism seed coating is 108-1010A spore living
Son/gram.
Further, the binder is selected from white carbon black, diatomite, kaolin, active carbon or combinations thereof object.Binder is normal
For processing the solid dosage forms such as pulvis, wettable powder, granule, effect is that dilution effective component is easy to process, and bonding is lived
Property substance, improve physicochemical character be easy to use.
Further, the protective agent is selected from maltodextrin, gum arabic, cyclodextrin or combinations thereof object.Protective agent is used
Protection and preparation in spray-drying process to seed and thallus formed after external protection film.
Further, the film forming agent is polyethylene glycol.
Further, the dispersing agent is sodium lignin sulfonate, calcium lignosulfonate, NNO or combinations thereof object.
Further, fermentation liquid obtains using the following method in the step (1):
The Trichoderma seed liquor of 5-10% is accessed in the fermentation medium, and seed concentration is 106-108A/mL, fermentation temperature
It 23~30 DEG C, ferments 90-150 hours, the fermentation liquid containing trichoderma chlamydospore is obtained, wherein the composition of the fermentation medium is pressed
Weight percent is: sucrose 0.2-1.5%, yeast extract 1-5%, molasses 2-8%, wheat bran 0.3-0.8%, soy meal 1.2-
5.5%, pH6.5.
Further, 90 DEG C of the spray drying EAT, sample introduction flow 55-80L/h, spray drying.
In addition, other active components can be also added in microorganism seed coating of the invention, for example as compensation
Chemical bactericide or nutriment, positive growth factor, this can be selected and be determined according to specific purposes.
On the other hand, microorganism seed coating made from the preparation method of the above-mentioned microorganism seed coating of the present invention is anti-
Control the application in green vegetables gray mold.
The invention discloses following technical effects:
The chlamydospore of Trichoderma is the resting spore that Trichoderma generates in adverse circumstance, there is stronger environmental resistance, thick
Wall spore once encounters suitable environmental condition and just sprouts generation mycelia.So using chlamydospore as preparation it is effective at
Dividing helps to solve the problems, such as that the Trichoderma spore holding time is short, is unable to large-scale production, is able to satisfy the requirement as biological pesticide.
Microorganism seed coating prepared by the present invention is the substance of similar microcapsules, and solid, liquid, gas are encapsulated in small
What is sealed is intracapsular, this capsule discharges capsule-core substance with can control rate under given conditions.Microencapsulation can make microorganism and
Seed is isolated from the environment, and Trichoderma and seed is enable to resist the influence of mechanical force and external environment, to keep biology for a long time
Activity.Moreover, microencapsulation, which can control thallus, is discharged into external environment, achieve the effect that sustained-release and controlled release, have it is good sun from
Sub- exchangeability, adsorptivity, water-retaining property, improve the surface of the seed microenvironment, and water storage is ventilative；The soda acid of seed ambient enviroment can be buffered
Degree keeps nutrient slowly-released, provides mineral element, promotes seedling stage plant growth.
The manufactured coating agent for seed according to above-mentioned weight ratio mixing, it is different while each component plays respective effect
Component between can also generate synergistic effect so that coating agent generate antibacterial, insect prevention, organic-inorganic nutrient, positive growth factor
Equal Related Components, can realize promote seed sprouting, preventing disease and pest, increase production and improve productivity etc. whiles, to region and Bamboo resource
Selectivity is wider, and can be used for the coating that salt resistance alkali, drought resisting, disease-resistant etc. have specific resistance seed, improves its corresponding resistance,
So that coating agent for seed has both specific aim and versatility simultaneously.More importantly each component of the coating agent is pure
Natural constituents, it is biodegradable, it is non-hazardous to people and animals and environment, meet the non-harmful requirement of agricultural production.
Specific embodiment
Below in conjunction with the embodiment of the present invention, technical scheme in the embodiment of the invention is clearly and completely described,
Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based in the present invention
Embodiment, every other embodiment obtained by those of ordinary skill in the art without making creative efforts, all
Belong to the scope of protection of the invention.
Embodiment 1
The present invention provides a kind of preparation method of microorganism seed coating, comprising the following steps:
(1) fermentation liquid of trichoderma chlamydospore is provided；The trichoderma includes trichoderma harzianum and trichoderma viride；
(2) fermentation liquid and dispersing agent, film forming agent and 5% protective agent containing trichoderma chlamydospore are mixed, 600r/min
Stirring 10-20 minutes；
(3) after the completion of step (2) are stirred, binder, green vegetables seed is added, the mass ratio of binder and green vegetables seed is
50:9,600r/min stir 5min；
(4) cold water of remaining protective agent quality 50% is added in remaining protective agent, stirs evenly, is heated to 60 DEG C and continues
For 2min to uniform liquid is formed, cooling is spare；
(5) protective agent of the fermentation liquid and step (4) that obtain step (3) is uniformly mixed, and mixed liquor is spray-dried,
Microorganism seed coating is made；
Wherein, the content of the binder is 19% (kg/L) of fermentation liquid, and protectant content is fermentation liquid
60% (kg/L), the content of the dispersing agent are 6% (kg/L) of fermentation liquid, and the content of the film forming agent is the 3% of fermentation liquid
(kg/L)；The content of trichoderma chlamydospore described in the microorganism seed coating is 109A spore living/gram.
In the present embodiment, binder: white carbon black (260 mesh) content is 10% (kg/L), the diatomite (350 of fermentation liquid
Mesh) content be fermentation liquid 9% (kg/L).
In the present embodiment, protective agent: maltodextrin content is 35% (kg/L) of fermentation liquid, gum arabic content is
25% (kg/L) of fermentation liquid.
In the present embodiment, film forming agent: polyethylene glycol.
In the present embodiment, dispersing agent: lignin sulfonic acid sodium content is 2% (kg/L), the calcium lignosulfonate of fermentation liquid
Content is 1% (kg/L) of fermentation liquid.
In the present embodiment, trichoderma harzianum is purchased from Shandong Wei Duofeng Bioisystech Co., Ltd, and trichoderma viride is purchased from
Jinan Jin Yuyuan Biotechnology Co., Ltd.
In the present embodiment, 90 DEG C of spray drying EAT, atomisation pressure 0.2MP, sample introduction flow 70L/h.
In the present embodiment, fermentation liquid obtains using the following method in the step (1):
The Trichoderma seed liquor of access 8% in the fermentation medium, seed concentration are 107A/mL, 25 DEG C of fermentation temperature,
Ventilation ratio 1:2, tank press 0.03MPa, ferment 125 hours, the fermentation liquid containing trichoderma chlamydospore are obtained, wherein the fermented and cultured
The composition of base is by weight: sucrose 0.9%, yeast extract 3%, molasses 5%, wheat bran 0.5%, soy meal 3.5%, pH6.5.
It is in logarithmic phase within fermentation 20-72 hours or so, fermentation liquid also starts to become sticky, mycelia raised growth.72-120
Hour is in stationary phase, and chlamydospore largely generates.At 125 hours or so, free chlamydospore reached at most, about 2 ×
108Cfu/ml, total chlamydospore 5 × 108A/mL.
The thallus survival rate of microorganism seed coating manufactured in the present embodiment is 85.36%, and seed viability 100% is high
It may be that the chlamydospore of thallus formation has resistance to high temperature that temperature spray drying, which still has the survival of seed thallus, and spray drying obtains
Microcapsules significant protective effect is played to intracapsular viable bacteria and seed
Microorganism seed made from embodiment 1 is coated and carries out storage at normal temperature, in storage in 12 months, under room temperature
The survival rate of microorganism seed coating lives spore rate still up to 84% after 90% or more, 24 month, and it is mitogenetic to be significantly higher than Trichoderma
The work spore rate (30%) of spore.
Embodiment 2
Using the seed for having microorganism seed coating made from embodiment 1 as experimental example, produced with BASF AG
50% Boscalid water dispersible granules and clear water are two reference examples.
Experiment carries out in greenhouse, and experimental example and reference examples are divided into three groups, and every group of 20 plants of green vegetables unify mould if 3 repetitions
Quasi- green vegetables field management environment.
As the result is shown: with microorganism seed coating green vegetables obtain gray mold probability be 5%, and illness seedling will not infect it is non-
Illness seedling；It is 15% that the green vegetables for applying 50% Boscalid water dispersible granules, which obtain gray mold probability, is sent out altogether in three groups of 60 plants of green vegetables
Existing 3 plants obtain gray mold to be infected by illness seedling；It is 45% that the green vegetables of application clear water, which obtain gray mold probability, wherein 5 plants are quilt
Illness seedling, which infects, obtains gray mold.
As it can be seen that microorganism seed coating has good control efficiency for green vegetables gray mold.Microorganism seed is coated
Trichoderma chlamydospore living body preparation, when preservation in coating in a dormant state.Apply field after, suitable temperature,
It sprouts, grow under damp condition, breeding, playing the antagonism to pathogen, effect stability.
Embodiment 3
Use the green vegetables seed with microorganism seed coating made from embodiment 1 for experimental group, and without coating green vegetables
Seed is control group, carries out growth experiment under the laboratory environment of no gray mold harm influence.
Experiment shows: in the case where identical growth management, the experimental group growing state with microorganism seed coating is complete
Stage is better than control group, and experimental group green vegetables are sprouted fastly, and growth is fast, and yield is high.
Embodiment described above is only that preferred embodiment of the invention is described, and is not carried out to the scope of the present invention
It limits, without departing from the spirit of the design of the present invention, those of ordinary skill in the art make technical solution of the present invention
Various changes and improvements, should all fall into claims of the present invention determine protection scope in.
Claims (8)
1. a kind of preparation method of microorganism seed coating, which comprises the following steps:
(1) fermentation liquid of trichoderma chlamydospore is provided；The trichoderma include trichoderma harzianum (Trichoderma viride) and
Trichoderma viride (Trichoderma viride)；
(2) fermentation liquid containing trichoderma chlamydospore is mixed with the protective agent of dispersing agent, film forming agent and 5% mass, 600r/min
Stirring 10-20 minutes；
(3) after the completion of step (2) are stirred, binder, seed is added, 600r/min stirs 5min；
(4) cold water of remaining protective agent quality 50% is added in remaining protective agent, stirs evenly, is heated to 60 DEG C of lasting 2min
To uniform liquid is formed, cooling is spare；
(5) protective agent of the fermentation liquid and step (4) that obtain step (3) is uniformly mixed, and mixed liquor is spray-dried, and is made
Microorganism seed coating；
Wherein, the content of the binder is the 8%-25% (kg/L) of fermentation liquid, and protectant content is fermentation liquid
60%-80% (kg/L), the content of the dispersing agent are the 4-10% (kg/L) of fermentation liquid, and the content of the film forming agent is fermentation
The 2-5% (kg/L) of liquid；The content of trichoderma chlamydospore described in the microorganism seed coating is 108-1010A spore living/
Gram.
2. a kind of preparation method of microorganism seed coating according to claim 1, which is characterized in that the binder choosing
From white carbon black, diatomite, kaolin, active carbon or combinations thereof object.
3. a kind of preparation method of microorganism seed coating according to claim 1, which is characterized in that the protective agent choosing
From maltodextrin, gum arabic, cyclodextrin or combinations thereof object.
4. a kind of preparation method of microorganism seed coating according to claim 1, which is characterized in that the film forming agent is
Polyethylene glycol.
5. a kind of preparation method of microorganism seed coating according to claim 1, which is characterized in that the dispersing agent is
Sodium lignin sulfonate, calcium lignosulfonate, NNO or combinations thereof object.
6. a kind of preparation method of microorganism seed coating according to claim 1, it is characterised in that:
Fermentation liquid obtains using the following method in the step (1):
The Trichoderma seed liquor of 5-10% is accessed in the fermentation medium, and seed concentration is 106-108A/mL, fermentation temperature 23~
It 30 DEG C, ferments 90-150 hours, the fermentation liquid containing trichoderma chlamydospore is obtained, wherein the composition of the fermentation medium is by weight
Percentage is: sucrose 0.2-1.5%, yeast extract 1-5%, molasses 2-8%, wheat bran 0.3-0.8%, soy meal 1.2-5.5%,
pH6.5。
7. a kind of preparation method of microorganism seed coating according to claim 1, which is characterized in that the spray drying
90 DEG C of inlet air temperature, sample introduction flow 55-80L/h, spray drying.
8. microorganism seed coating made from the preparation method of the described in any item microorganism seed coatings of claim 1-7 is anti-
Control the application in green vegetables gray mold.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910299546.4A CN109892327A (en) | 2019-04-15 | 2019-04-15 | A kind of preparation method and applications of microorganism seed coating |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910299546.4A CN109892327A (en) | 2019-04-15 | 2019-04-15 | A kind of preparation method and applications of microorganism seed coating |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109892327A true CN109892327A (en) | 2019-06-18 |
Family
ID=66955818
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910299546.4A Pending CN109892327A (en) | 2019-04-15 | 2019-04-15 | A kind of preparation method and applications of microorganism seed coating |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109892327A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103039439A (en) * | 2011-10-11 | 2013-04-17 | 上海万力华生物科技有限公司 | Seed coating agent of Trichoderma fungi and preparation method thereof |
| KR20140083122A (en) * | 2012-12-24 | 2014-07-04 | 농업회사법인주식회사 오상킨섹트 | Chlamydospore formation technology and microorganism agents using trichoderma sp. ok-1 |
| CN105794856A (en) * | 2014-12-31 | 2016-07-27 | 华东理工大学 | Trichoderma sp. chlamydospore microcapsule and its preparation method and use |
| CN106386869A (en) * | 2016-08-31 | 2017-02-15 | 上海交通大学 | Trichoderma seed coating as well as preparation method and application thereof |
| CN106818887A (en) * | 2016-12-30 | 2017-06-13 | 新昌县派特普科技有限公司 | A kind of trichoderma fungi seed coat agent and preparation method thereof |
-
2019
- 2019-04-15 CN CN201910299546.4A patent/CN109892327A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103039439A (en) * | 2011-10-11 | 2013-04-17 | 上海万力华生物科技有限公司 | Seed coating agent of Trichoderma fungi and preparation method thereof |
| KR20140083122A (en) * | 2012-12-24 | 2014-07-04 | 농업회사법인주식회사 오상킨섹트 | Chlamydospore formation technology and microorganism agents using trichoderma sp. ok-1 |
| CN105794856A (en) * | 2014-12-31 | 2016-07-27 | 华东理工大学 | Trichoderma sp. chlamydospore microcapsule and its preparation method and use |
| CN106386869A (en) * | 2016-08-31 | 2017-02-15 | 上海交通大学 | Trichoderma seed coating as well as preparation method and application thereof |
| CN106818887A (en) * | 2016-12-30 | 2017-06-13 | 新昌县派特普科技有限公司 | A kind of trichoderma fungi seed coat agent and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Locatelli et al. | Development of Trichoderma sp. formulations in encapsulated granules (CG) and evaluation of conidia shelf-life | |
| TWI379642B (en) | ||
| US5074902A (en) | Granular products containing fungi encapsulated in a wheat gluten matrix for biological control of weeds | |
| CN105794856B (en) | Trichoderma harzianum chlamydospore microcapsule formulations and its preparation and application | |
| CN105875653B (en) | A kind of beauveria bassiana microbial pesticide and preparation method thereof | |
| CN103964969B (en) | A kind of Chinese medicinal materials plantation substrate and application thereof | |
| CN101502271B (en) | Method for producing fungal granular formulation for preventing and treating pest | |
| US10807920B2 (en) | Trichoderma-derived selenium nanoparticles foliar fertilizer for reducing crop fungal diseases and toxin contamination | |
| CN109776217A (en) | A kind of biological bacteria composite foliage fertilizer and its preparation method and application | |
| CN102027998A (en) | Bacillus spore and trichoderma chlamydospore compound preparation and preparation method and application thereof | |
| GB2577250A (en) | Insect-pathogenic fungus, spores, composition and use of same | |
| CN108513988A (en) | Microorganism composite pesticide | |
| EP4433573A1 (en) | Increased biological agent performance and reduced variation across areas of application | |
| CN108770845B (en) | Dispersible solid preparation and its preparation method and application for preventing fruit shedding | |
| CN106083417A (en) | A kind of improvement soil biosurfactant compositions | |
| Sarrocco et al. | Seeds encapsulation in calcium alginate pellets | |
| CN103609560A (en) | Suspended seed coating for preventing and treating diseases of corn leaves, spikes and stems and underground injurious insects | |
| CN109892327A (en) | A kind of preparation method and applications of microorganism seed coating | |
| CN106879585A (en) | Trichoderma brassin lactones wettable powder and its preparation method and application | |
| JP5779746B2 (en) | Granules containing filamentous fungi and method for preparing the same | |
| JP2001078751A (en) | Storage of microbial preparation and microorganism | |
| CN103875657B (en) | A kind of uvioresistant agricultural water dispersible granule containing natamycin and preparation method thereof | |
| JP7177601B2 (en) | Method for producing microbial pesticide | |
| CN116491503A (en) | Stable microbial composition and drying process | |
| CN109022300A (en) | Straw decomposing inoculant and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190618 |
|
| RJ01 | Rejection of invention patent application after publication |