CN109874952A - A kind of shellfish meat Anti-fatigue drink and preparation method thereof - Google Patents

A kind of shellfish meat Anti-fatigue drink and preparation method thereof Download PDF

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CN109874952A
CN109874952A CN201910240288.2A CN201910240288A CN109874952A CN 109874952 A CN109874952 A CN 109874952A CN 201910240288 A CN201910240288 A CN 201910240288A CN 109874952 A CN109874952 A CN 109874952A
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parts
shellfish meat
hippocampus
added
fatigue
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易湘茜
米顺利
邓家刚
高程海
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Guangxi University of Chinese Medicine
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Guangxi University of Chinese Medicine
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Abstract

The present invention provides a kind of shellfish meat Anti-fatigue drink and preparation method thereof, belongs to food-making technology field, which includes following components according to parts by weight: 120~160 parts of shellfish meat, 5~10 parts of hippocampus, 5~8 parts of American Ginseng, 5~15 parts of rhizoma polygonati, 10~15 parts of pawpaw, 10~20 parts of fructus lycii, 15~25 parts of pueraria lobata, 10~15 parts of hawthorn, 10~20 parts of lotus seeds, 10~20 parts of arillus longan, 3~8 parts of dried orange peel.The drink is prepared using shellfish meat as primary raw material in conjunction with a variety of medicine and food raw materials, uniform color, without precipitating, it gives off a strong fragrance, free from extraneous odour, delicate mouthfeel, sour-sweet degree are moderate, no bitter taste, unique flavor, and antifatigue Deng health-care effects are improved, it can satisfy different consumer demands.The preparation method of the shellfish meat Anti-fatigue drink, simple process, effective component are high, are suitble to industrialized production.

Description

A kind of shellfish meat Anti-fatigue drink and preparation method thereof
Technical field
The present invention relates to technical field of food preparation, and in particular to a kind of shellfish meat Anti-fatigue drink and preparation method thereof.
Background technique
Modern society's life rhythm becomes to be getting faster, and has brought huge survival pressure, the ratio of tired crowd Example constantly expands, and many people are in sub-health state because of over fatigue, has seriously affected working efficiency and life matter Amount resists fatigue and has become a kind of society need.Restore physical efficiency as early as possible, people could complete the following laborious task and heavy Learning tasks.The physical condition of people in order to better improve meets normal life and work requirement, with health-care effect Anti-fatigue drink is come into being.
Protein and amino acid all rich in shellfish meat, especially also contain taurine, and taurine is nonessential ammonia It is sour, it is related to the synthesis of internal cysteine, bile secretion and absorption can be promoted, be conducive to developing infant brain, nerve Conduction, perfect, calcium the absorption and the digestion and absorption of lipid material of visual function, are the very high substances of nutritive value.From Can extract glycosaminoglycan, glycoprotein, glycosaminoglycan etc. in shellfish meat, these substances all have very strong activity, it can keep fit and healthy, Immunity is improved, and has certain health-care effect to diseases such as invigorating the spleen shield heart, activating microcirculation and removing stasis medicinal, atherosclerosis, thrombotic diseases. Hippocampus sweet-salty, property are slow, enter liver, kidney two warp, have liver-kidney tonifying, establishing-Yang tranquilizing the mind, regulating the qi flowing in the channels, promoting blood circulation, relaxing tendons and activating collaterals, Zhichuan flat The functions such as asthma.Application No. is the patents of invention of CN201010500964.4 to disclose a kind of anti-fatigue oral liquids by using Pinctada martensii dunker meats Production method, the stirring degreasings of No. 6 light petrols are added except fishy smell, then enzymatic hydrolysis is added auxiliary material and is made, and the oral liquid component is single, Antifatigue effect is limited, and taste is single, except raw meat effect is general, is not able to satisfy different consumer demands.Therefore, a kind of battalion is developed Support value height, the anti-fatigue beverage of unique flavor is needed as market.
Summary of the invention
Goal of the invention of the invention first is that, in view of the above-mentioned problems, a kind of shellfish meat Anti-fatigue drink is provided, based on shellfish meat Raw material is wanted, in conjunction with a variety of medicine and food raw materials, antifatigue Deng health-care effects, and unique flavor is improved, can satisfy difference Consumer demand.
In order to achieve the above objectives, the technical scheme adopted by the invention is that:
A kind of shellfish meat Anti-fatigue drink, is prepared from the following raw materials according to parts by weight:
120~160 parts of shellfish meat, 5~10 parts of hippocampus, 5~8 parts of American Ginseng, 5~15 parts of rhizoma polygonati, 10~15 parts of pawpaw, fructus lycii 10~20 parts, 15~25 parts of pueraria lobata, 10~15 parts of hawthorn, 10~20 parts of lotus seeds, 10~20 parts of arillus longan, 3~8 parts of dried orange peel.
A kind of preferred shellfish meat Anti-fatigue drink, the shellfish meat are Pinctada martensii meat or oyster meat.
Another goal of the invention of the invention is, in view of the above-mentioned problems, a kind of preparation method of shellfish meat Anti-fatigue drink is provided, Simple process, effective component are high, are suitble to industrialized production.
A kind of production method of shellfish meat Anti-fatigue drink, comprising the following steps:
S1. shellfish meat effective component is extracted
1) deodorant: shellfish meat is weighed according to the parts by weight, water progress homogenized is added, obtain homogenate;According to shared described Shellfish meat mass ratio, 2~5% gingers are added in Xiang Suoshu homogenate, and 1~2% yeast carries out defishying and obtains pretreatment fluid.
2) it extracts: weighing pawpaw according to the parts by weight, water is added and carries out homogenized and is added in the pretreatment fluid, The compound protease that shellfish meat quality 1~3% is added carries out ultrasonic wave extraction, then proceedes to digest in 50~65 DEG C of waters bath with thermostatic control 1~2h obtains enzymolysis liquid, finally that enzymolysis liquid is cooling, and centrifugation obtains shellfish meat extracting solution.
S2. active ingredients from sea horse extracts
1) it pre-processes: weighing hippocampus according to the parts by weight, hippocampus internal organ are removed, clean up, dry, crushing extra large Horse material is spare.
2) it extracts: the water of 3~5 times of hippocampus material quality is added, the compound protease that hippocampus material quality 1~3% is added carries out Enzymatic hydrolysis, 50 DEG C~55 DEG C at a temperature of, after being stirred to react 6~8h, be heated to 100 DEG C of enzyme deactivations, filter, isolated extracting solution And filter residue.
3) active carbon of extracting solution mass percent 0.5~0.7%, stirring 5 deodorant: will be added while hot in the extracting solution Minute, filtering obtains hippocampus extracting solution.
S3. American Ginseng, fructus lycii and kudzu vine root extract
1) water mentions: weigh American Ginseng, fructus lycii and pueraria lobata according to the parts by weight, then be added water ultrasonic-leaching 30~ 120min, 40 mesh screens obtain filtrate and filter residue, and filter residue is repeated above step 1~2 time, obtained filtrate is merged to obtain water Extract.
2) it ferments: Aqueous extracts being accessed into fermentation seed liquid, fermentation obtains fermentation liquid.
S4. other feedstock processings
Rhizoma polygonati, hawthorn, lotus seeds, arillus longan and dried orange peel are weighed according to the parts by weight, water ultrasonic-leaching 30 is then added ~120min, 40~80 mesh screens obtain filtrate and filter residue, and filter residue is repeated above step 1~2 time, and obtained filtrate is closed And obtain Chinese medicine extract;
S5 mixing
Shellfish meat extracting solution, hippocampus extracting solution, fermentation liquid and Chinese medicine that step 1, step 2, step 3 and step 4 obtain are mentioned It takes liquid to be mixed evenly, sterilize, shellfish meat Anti-fatigue drink is made.
Preferably, in the step S1 and step S2, the compound protease is trypsase and subtilopeptidase A.
Preferably, the complex enzyme includes: 200~500U/g of trypsase and hay bacillus according to every gram of homogenate calculating 20~100U/g of protease.
Preferably, in the step S1, the defishying is 3~4h of deodorant at 4~15 DEG C.
Preferably, in the step S3 and S4, the working frequency of the ultrasonic wave is 25~35kHz, ultrasonic intensity is 300~500W, temperature are 50~65 DEG C.
It preferably, include lactobacillus, Bifidobacterium, acetic acid bacteria and saccharomycete in fermentation seed liquid in the step S3.
Preferably, in the step S3, fermentation condition are as follows: 30~50 DEG C, 5~20d of fermentation period.
Due to the adoption of the above technical scheme, the invention has the following advantages:
1. shellfish meat Anti-fatigue drink of the invention, in conjunction with a variety of medicine and food raw materials, is improved using shellfish meat as primary raw material Antifatigue equal health-care effects, and unique flavor, can satisfy different consumer demands.
It can extract glycosaminoglycan, glycoprotein, glycosaminoglycan etc. from shellfish meat, these substances all have very strong activity, right The diseases such as invigorating the spleen shield heart, activating microcirculation and removing stasis medicinal, atherosclerosis, thrombotic disease have certain health-care effect.Also containing packet in shellfish meat Include 19 kinds of amino acid such as the various essential amino acids of human body and a large amount of taurine and largely be easily absorbed by the body calcium, magnesium, phosphorus, Body can be improved animal experiment proves that it has effects that reducing blood lipid and antifatigue etc. in 28 kinds of elements such as potassium, iron, zinc, germanium, selenium The activity of interior superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) increases white blood cell and platelet count Mesh enhances body immunity and anti-aging etc..Hippocampus is a kind of rare Chinese medicine, have keep fit and healthy, tonifying kidney and strengthening yang, relaxing muscles and tendons The medicinal functions such as active, anti-inflammatory analgetic, tranquilizing and allaying excitement, relieving cough and asthma more have especially for the disease for the treatment of nervous system Effect mainly contains hippocampus polysaccharide, crude protein, lipid (containing phosphatide, steroidal etc.) etc., the albumen of hippocampus through document announcement in hippocampus Enzymolysis liquid has the effects of apparent antifatigue, raising immunity, resisting stress, analgesia.
In addition, addition American Ginseng, rhizoma polygonati, pawpaw, fructus lycii, pueraria lobata, hawthorn, lotus seeds, arillus longan and dried orange peel, improve mouthfeel and Improvement effect.Polysaccharide rich in a variety of Chinese herbal medicines, such as: polysaccharides, Siberian solomonseal rhizome polysaccharide etc., it is tired with significantly resisting The effect of labor, resist oxygen lack.Glycoside compound also rich in, such as American ginseng saponin, Puerarin xyloside and rough melic herb glucoside, The effect of Central nervous system and antifatigue effect such as adjust nervous excitation and inhibited to the dual regulation of human body The balance of journey and the adaptation of adverse environment etc..Antifatigue effect can be significantly improved.
Wherein, the saponin in American Ginseng can effectively enhance nervous centralis, reach it is with all worries set aside with fixed attention, eliminate fatigue, enhancing note The effects of recalling power is applicable to the symptoms such as insomnia, irritated, memory loss and senile dementia.Rhizoma polygonati has tonifying spleen, moistening lung and producing body fluid Effect.Polysaccharides in fructus lycii, which has, promotes immune, anti-aging, antitumor, removing free radical, antifatigue, anti-radiation, guarantor The effects of liver, reproductive function protection and improvement.The effect of pawpaw is calming the liver relaxing muscles and tendons, and stomach function regulating dampness elimination is used for arthritis with fixed pain caused by dampness muscular constricture, and waist and knee is closed Section acid weight pain, spasm of vomiting and diarrhoea, tinea pedis oedema.Pueraria lobata generates heat for exterior syndrome, and stiff nape and back, measles without adequate eruption, pyreticosis is thirsty, the deficiency of Yin It the symptoms such as quenches one's thirst.Lotus seeds have the effect of tonifying spleen and stopping diarrhea, stop-band, kidney-nourishing arresting seminal emission, mental-tranquilization.Arillus longan has tonifying heart and spleen, The effect of nourishing blood and tranquilization;It can be used for insufficiency of vital energy and blood, palpitation and severe palpitation.Dried orange peel has invigorating middle warmer and replenishing QI, purging fire for removing toxin, and coordinating the drug actions of a prescription Effect.
2. the preparation method of shellfish meat Anti-fatigue drink of the invention, handles effective component extracting for shellfish meat and hippocampus respectively, It can be improved active constituent content, improve the antifatigue effect of drink.
Wherein, shellfish meat is first used to ginger and yeast defishying, improves the whole mouthfeel of drink, pawpaw is then added and is answered Hop protein enzyme is digested, and obtained extract generates more branched chain amino acids and small peptide, significantly improves product quality.
Complex enzyme include compound protease be trypsase, subtilopeptidase A and pawpaw in papain. Protein breakdown can be polypeptide even amino acid by papain;Trypsase is a kind of silk extracted from the pancreas of ox, sheep, pig Propylhomoserin proteolytic enzyme can cut off the carboxyl side in polypeptide chain in lysine and arginine residues.Subtilopeptidase A is Extracellular alkali protease secreted by bacillus, catalyzing hydrolysis protein are amino acid, promote the synthesis of polypeptide.Phase For vegetal papain, trypsase effect is more significant.Use trypsase, papain, hay bacillus egg White enzyme hydrolysis result is obvious, and three's mixing can sufficiently combine the excellent of vegetable protein enzyme, animal protein enzyme and bacterialprotease Point, obtained extract generate more amino acid and small peptide, improve drink quality.
Hippocampus is first subjected to enzymolysis and extraction, then active carbon removes raw meat, improves the whole mouthfeel and antifatigue effect of drink.
3. the production method of shellfish meat Anti-fatigue drink of the invention ferments American Ginseng, fructus lycii and pueraria lobata Aqueous extracts, Compared with simple Aqueous extracts, taste is sour-sweet, and mouthfeel more preferably, and is more advantageous to absorption of human body, to improve antifatigue effect.
4. the production method of shellfish meat Anti-fatigue drink of the invention, simple process, effective component are high, are suitble to industry metaplasia It produces.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to Limit the present invention.
Embodiment 1
A kind of shellfish meat Anti-fatigue drink, is prepared from the following raw materials according to parts by weight:
150 parts of Pinctada martensii meat, 6 parts of hippocampus, 6 parts of American Ginseng, 10 parts of rhizoma polygonati, 12 parts of pawpaw, 15 parts of fructus lycii, pueraria lobata 20 Part, 12 parts of hawthorn, 15 parts of lotus seeds, 15 parts of arillus longan, 5 parts of dried orange peel.
Production method, comprising the following steps:
S1. shellfish meat effective component is extracted
1) deodorant: the water progress homogenized that 2 times of quality are added in shellfish meat is weighed according to the parts by weight, obtains homogenate;It presses According to the shared shellfish meat mass ratio, 3% ginger is added in Xiang Suoshu homogenate, 1.5% yeast, at 10 DEG C, deodorant 3.5h is obtained pre- Treatment fluid.
2) it extracts: weighing pawpaw according to the parts by weight, the water that 1 times of quality is added carries out homogenized and is added described pre- In treatment fluid, the compound protease that homogenate quality 2% is added carries out ultrasonic wave extraction, then proceedes in 55 DEG C of waters bath with thermostatic control Enzymatic hydrolysis 1.5h obtains enzymolysis liquid, finally that enzymolysis liquid is cooling, and centrifugation obtains shellfish meat extracting solution.
The compound protease is trypsase and subtilopeptidase A.The complex enzyme is calculated according to every gram of homogenate It include: trypsase 400U/g and subtilopeptidase A 60U/g.
S2. active ingredients from sea horse extracts
1) it pre-processes: weighing hippocampus according to the parts by weight, hippocampus internal organ are removed, clean up, dry, crushing extra large Horse material is spare.
2) it extracts: the water of 4 times of hippocampus material quality is added, the compound protease that hippocampus material quality 1~3% is added carries out enzyme Solution, 53 DEG C at a temperature of, after being stirred to react 7h, be heated to 100 DEG C of enzyme deactivations, filter, isolated extracting solution and filter residue.
The compound protease is trypsase and subtilopeptidase A.The complex enzyme is calculated according to every gram of homogenate It include: trypsase 300U/g and subtilopeptidase A 80U/g.
3) deodorant: will be added while hot the active carbon of extracting solution mass percent 0.6% in the extracting solution, stir 5 minutes, Filtering, obtains hippocampus extracting solution.
S3. American Ginseng, fructus lycii and kudzu vine root extract
1) water mentions: weighing American Ginseng, fructus lycii and pueraria lobata according to the parts by weight, the water ultrasonic wave of 4 times of quality is then added 60min is extracted, 40 mesh screens obtain filtrate and filter residue, and filter residue is repeated above step 2 times, obtained filtrate is merged to obtain water Extract.
The working frequency of the ultrasonic wave is 30kHz, ultrasonic intensity 400W, temperature are 55 DEG C.
2) it ferments: Aqueous extracts sugaring liquid being accessed into fermentation seed liquid, fermentation obtains fermentation liquid.
It include lactobacillus, Bifidobacterium, acetic acid bacteria and saccharomycete in fermentation seed liquid.Fermentation condition are as follows: 40 DEG C, fermentation week Phase 10d.
S4. other feedstock processings
Rhizoma polygonati, hawthorn, lotus seeds, arillus longan and dried orange peel are weighed according to the parts by weight, water ultrasonic-leaching is then added 60min, 60 mesh screens obtain filtrate and filter residue, by filter residue repeat above step 2 times, by obtained filtrate merge Chinese medicine mentions Take liquid;The working frequency of the ultrasonic wave is 25kHz, ultrasonic intensity 500W, temperature are 50 DEG C.
S5 mixing
Shellfish meat extracting solution, hippocampus extracting solution, fermentation liquid and Chinese medicine that step 1, step 2, step 3 and step 4 obtain are mentioned It takes liquid to be mixed evenly, sterilize, shellfish meat Anti-fatigue drink is made.
Embodiment 2
A kind of shellfish meat Anti-fatigue drink, is prepared from the following raw materials according to parts by weight:
120 parts of Pinctada martensii meat, 10 parts of hippocampus, 5 parts of American Ginseng, 15 parts of rhizoma polygonati, 15 parts of pawpaw, 12 parts of fructus lycii, pueraria lobata 20 parts, 15 parts of hawthorn, 10 parts of lotus seeds, 20 parts of arillus longan, 3 parts of dried orange peel.
Production method, comprising the following steps:
S1. shellfish meat effective component is extracted
1) deodorant: shellfish meat is weighed according to the parts by weight, water progress homogenized is added, obtain homogenate;According to shared described 5% ginger is added in Xiang Suoshu homogenate in shellfish meat mass ratio, and 1% yeast, at 15 DEG C, deodorant 3h obtains pretreatment fluid.
2) it extracts: weighing pawpaw according to the parts by weight, water is added and carries out homogenized and is added in the pretreatment fluid, The compound protease that shellfish meat quality 3% is added carries out ultrasonic wave extraction, then proceedes to the enzymatic hydrolysis 1h in 65 DEG C of waters bath with thermostatic control and obtains enzyme Liquid is solved, finally that enzymolysis liquid is cooling, centrifugation obtains shellfish meat extracting solution.
The compound protease is trypsase and subtilopeptidase A.The complex enzyme is calculated according to every gram of homogenate It include: trypsase 500U/g and subtilopeptidase A 100U/g.
S2. active ingredients from sea horse extracts
1) it pre-processes: weighing hippocampus according to the parts by weight, hippocampus internal organ are removed, clean up, dry, crushing extra large Horse material is spare.
2) it extracting: the water of 3 times of hippocampus material quality is added, the compound protease that hippocampus material quality 3% is added is digested, At a temperature of 55 DEG C, after being stirred to react 6h, 100 DEG C of enzyme deactivations are heated to, are filtered, isolated extracting solution and filter residue.
The compound protease is trypsase and subtilopeptidase A.The complex enzyme is calculated according to every gram of homogenate It include: trypsase 200U/g and subtilopeptidase A 100U/g.
3) deodorant: will be added while hot the active carbon of extracting solution mass percent 0.7% in the extracting solution, stir 5 minutes, Filtering, obtains hippocampus extracting solution.
S3. American Ginseng, fructus lycii and kudzu vine root extract
1) water mentions: weighing American Ginseng, fructus lycii and pueraria lobata according to the parts by weight, water ultrasonic-leaching is then added 120min, 40 mesh screens obtain filtrate and filter residue, by filter residue repeat above step 1 time, by obtained filtrate merge water mentions Liquid.
The working frequency of the ultrasonic wave is 35kHz, ultrasonic intensity 300W, temperature are 65 DEG C.
2) it ferments: Aqueous extracts sugaring liquid being accessed into fermentation seed liquid, fermentation obtains fermentation liquid.
It include lactobacillus, Bifidobacterium, acetic acid bacteria and saccharomycete in fermentation seed liquid.Fermentation condition are as follows: 50 DEG C, fermentation week Phase 8d.
S4. other feedstock processings
Rhizoma polygonati, hawthorn, lotus seeds, arillus longan and dried orange peel are weighed according to the parts by weight, water ultrasonic-leaching is then added 120min, 40~80 mesh screens obtain filtrate and filter residue, by filter residue repeat above step 1 time, by obtained filtrate merge in Medicament extracting solution;The working frequency of the ultrasonic wave is 35kHz, ultrasonic intensity 500W, temperature are 50 DEG C.
S5 mixing
Shellfish meat extracting solution, hippocampus extracting solution, fermentation liquid and Chinese medicine that step 1, step 2, step 3 and step 4 obtain are mentioned It takes liquid to be mixed evenly, sterilize, shellfish meat Anti-fatigue drink is made.
Embodiment 3
A kind of shellfish meat Anti-fatigue drink, is prepared from the following raw materials according to parts by weight:
160 parts of oyster meat, 5 parts of hippocampus, 8 parts of American Ginseng, 5 parts of rhizoma polygonati, 10 parts of pawpaw, 20 parts of fructus lycii, 25 parts of pueraria lobata, hawthorn 10 parts, 20 parts of lotus seeds, 10 parts of arillus longan, 8 parts of dried orange peel.
The preparation method is the same as that of Example 1.
Embodiment 4
A kind of shellfish meat Anti-fatigue drink, is prepared from the following raw materials according to parts by weight:
130 parts of Pinctada martensii meat, 8 parts of hippocampus, 6 parts of American Ginseng, 12 parts of rhizoma polygonati, 12 parts of pawpaw, 18 parts of fructus lycii, pueraria lobata 16 Part, 12 parts of hawthorn, 16 parts of lotus seeds, 12 parts of arillus longan, 6 parts of dried orange peel.
The preparation method is the same as that of Example 1.
Comparative example 1
A kind of shellfish meat Anti-fatigue drink, is prepared from the following raw materials according to parts by weight:
150 parts of Pinctada martensii meat, 6 parts of American Ginseng, 10 parts of rhizoma polygonati, 12 parts of pawpaw, 15 parts of fructus lycii, 20 parts of pueraria lobata, hawthorn 12 parts, 15 parts of lotus seeds, 15 parts of arillus longan, 5 parts of dried orange peel.
Production method compared with Example 1, does not have step S2.
Comparative example 2
A kind of shellfish meat Anti-fatigue drink, is prepared from the following raw materials according to parts by weight:
150 parts of Pinctada martensii meat, 6 parts of hippocampus, 6 parts of American Ginseng, 10 parts of rhizoma polygonati, 12 parts of pawpaw, 15 parts of fructus lycii, pueraria lobata 20 Part, 12 parts of hawthorn, 15 parts of lotus seeds, 15 parts of arillus longan, 5 parts of dried orange peel.
Production method does not have deodorant step compared with Example 1 in step S1.
Comparative example 3
A kind of shellfish meat Anti-fatigue drink, is prepared from the following raw materials according to parts by weight:
150 parts of Pinctada martensii meat, 6 parts of hippocampus, 6 parts of American Ginseng, 10 parts of rhizoma polygonati, 12 parts of pawpaw, 15 parts of fructus lycii, pueraria lobata 20 Part, 12 parts of hawthorn, 15 parts of lotus seeds, 15 parts of arillus longan, 5 parts of dried orange peel.
Production method, compared with Example 1, compound protease is trypsase in step S1.
Comparative example 4
A kind of shellfish meat Anti-fatigue drink, is prepared from the following raw materials according to parts by weight:
150 parts of Pinctada martensii meat, 6 parts of hippocampus, 6 parts of American Ginseng, 10 parts of rhizoma polygonati, 12 parts of pawpaw, 15 parts of fructus lycii, pueraria lobata 20 Part, 12 parts of hawthorn, 15 parts of lotus seeds, 15 parts of arillus longan, 5 parts of dried orange peel.
Production method, compared with Example 1, compound protease is not added in hippocampus in step S2, directly carries out water and mentions.
Comparative example 5
A kind of shellfish meat Anti-fatigue drink, is prepared from the following raw materials according to parts by weight:
150 parts of Pinctada martensii meat, 6 parts of hippocampus, 6 parts of American Ginseng, 10 parts of rhizoma polygonati, 12 parts of pawpaw, 15 parts of fructus lycii, pueraria lobata 20 Part, 12 parts of hawthorn, 15 parts of lotus seeds, 15 parts of arillus longan, 5 parts of dried orange peel.
Production method does not have fermentation step in step S3 compared with Example 1.
One, mouthfeel is verified:
Shellfish meat Anti-fatigue drink is prepared according to the preparation method of embodiment 1-4, comparative example 1-5 respectively, by shellfish obtained by each group Meat Anti-fatigue drink carries out color (30 points), 3 smell (30 points), mouthfeel (40 points) performance indicators are appraised through comparison, and whens comparation and assessment presses According to the standard of table 1,20 valuation officers is asked to carry out objective marking, each index is made even respectively, and last acquired results are shown in Table 2.
1 evaluation criterion of table
2 interpretation of result of table
Drink Color (divides) Smell (divides) Mouthfeel (is divided) Comprehensive (dividing)
Embodiment 1 28 28 37 93
Embodiment 2 28 26 37 91
Embodiment 3 28 27 37 92
Embodiment 4 28 28 36 92
Comparative example 1 28 27 37 92
Comparative example 2 27 21 34 80
Comparative example 3 26 26 35 87
Comparative example 4 27 26 35 88
Comparative example 5 25 25 32 82
From the data of table 2,1-4 can be seen that shellfish meat Anti-fatigue drink of the invention in conjunction with the embodiments, and uniform color, nothing are heavy It forms sediment, gives off a strong fragrance, free from extraneous odour, delicate mouthfeel, sour-sweet degree are moderate, no bitter taste, unique flavor, and improve antifatigue equal health cares effect Fruit can satisfy different consumer demands.
In conjunction with the embodiments 1 and comparative example 2 it can be seen that, wherein shellfish meat first used to ginger and yeast defishying, improves drink The whole mouthfeel of product.
In conjunction with the embodiments 1 and comparative example 5 as can be seen that American Ginseng, fructus lycii and pueraria lobata Aqueous extracts are fermented, and it is simple Aqueous extracts are compared, and taste is sour-sweet, and mouthfeel is more preferably.
Two, antifatigue compliance test result:
Experimental animal feeding and grouping:
Qualified NIH mouse 180 of health are chosen, are randomly divided into 9 groups, every group 20, sub-cage rearing, the weight of animals 18- 22 g, are respectively adopted the drink continuous gavage of embodiment 1-4 and comparative example 1-5 30 days.
Power exhausts swimming test:
After last dose, mouse is allowed to rest 30min, and swimming time is then carried out in small swimming pool Measurement, the size of small swimming pool are 50cm × 50cm × 40cm, and depth of water 30cm, water temperature is 25 ± 1 DEG C.Constantly stir in pond Water to guarantee that mouse ceaselessly moves about.Head still normally cannot be stretched out the water surface by 7s after mouse head submerged, then recognized For mouse at this time, entrance power exhausts state, records the time for the state that exhausts of being swum since mouse to power, which is that power exhausts swimming Time.Statistical data simultaneously calculates average value, the results are shown in Table 3.
3 swimming time of table statistics
Interpretation of result:
Swimming time is the key index for evaluating resisting kinetic fatigue.From the data of table 3,1-4 can be in conjunction with the embodiments Find out, shellfish meat Anti-fatigue drink of the invention, antifatigue significant effect.
In conjunction with the embodiments 1 and comparative example 1 as can be seen that addition hippocampus can increase substantially the antifatigue effect of drink.
In conjunction with the embodiments 1 and comparative example 3 as can be seen that complex enzyme include compound protease be trypsase, hay bacillus Protease, then there are also the papains in pawpaw.Three's mixing can sufficiently combine vegetable protein enzyme, animal protein enzyme And the advantages of bacterialprotease, obtained extract generates more amino acid and small peptide, relatively single trypsase resist tired Labor effect is more significant.
In conjunction with the embodiments 1 and comparative example 4 as can be seen that by hippocampus enzymolysis and extraction, mention, can be extracted more relative to direct water More hippocampus polysaccharide, crude protein, lipid (containing phosphatide, steroidal etc.) ingredient, and more amino acid, are more advantageous to absorption, thus Antifatigue effect is more significant.
In conjunction with the embodiments 1 and comparative example 5 as can be seen that American Ginseng, fructus lycii and pueraria lobata Aqueous extracts are fermented, and it is simple Aqueous extracts are compared, and taste is sour-sweet, and mouthfeel more preferably, and is more advantageous to absorption of human body, to improve antifatigue effect.
In conclusion shellfish meat Anti-fatigue drink of the invention, using shellfish meat and hippocampus as primary raw material, in conjunction with a variety of medium-height grass Medicine, and different processing and extracting method are combined, antifatigue Deng health-care effects, and unique flavor are improved, can satisfy not Same consumer demand.
Above description is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair Bright patent claim, it is all the present invention suggested by technical spirit under completed same changes or modifications change, should all belong to In the covered the scope of the patents of the present invention.

Claims (9)

1. a kind of shellfish meat Anti-fatigue drink, which is characterized in that be prepared from the following raw materials according to parts by weight:
120~160 parts of shellfish meat, 5~10 parts of hippocampus, 5~8 parts of American Ginseng, 5~15 parts of rhizoma polygonati, 10~15 parts of pawpaw, fructus lycii 10~ 20 parts, 15~25 parts of pueraria lobata, 10~15 parts of hawthorn, 10~20 parts of lotus seeds, 10~20 parts of arillus longan, 3~8 parts of dried orange peel.
2. shellfish meat Anti-fatigue drink according to claim 1, which is characterized in that the shellfish meat is Pinctada martensii meat or male Oyster meat.
3. according to the production method of shellfish meat Anti-fatigue drink described in any claim 1-2, which is characterized in that including following step It is rapid:
S1. shellfish meat effective component is extracted
1) deodorant: shellfish meat is weighed according to the parts by weight, water progress homogenized is added, obtain homogenate;According to the shared shellfish meat Mass ratio, 2~5% gingers are added in Xiang Suoshu homogenate, and 1~2% yeast carries out defishying and obtains pretreatment fluid;
2) it extracts: weighing pawpaw according to the parts by weight, water is added and carries out homogenized and is added in the pretreatment fluid, is added The compound protease of shellfish meat quality 1~3% carries out ultrasonic wave extraction, then proceed in 50~65 DEG C of waters bath with thermostatic control enzymatic hydrolysis 1~ 2h obtains enzymolysis liquid, finally that enzymolysis liquid is cooling, and centrifugation obtains shellfish meat extracting solution;
S2. active ingredients from sea horse extracts
1) it pre-processes: weighing hippocampus according to the parts by weight, hippocampus internal organ are removed, clean up, dry, crushing and to obtain hippocampus material It is spare;
2) it extracting: the water of 3~5 times of hippocampus material quality is added, the compound protease that hippocampus material quality 1~3% is added is digested, 50~55 DEG C at a temperature of, after being stirred to react 6~8h, be heated to 100 DEG C of enzyme deactivations, filter, isolated extracting solution and filter residue;
3) deodorant: will be added while hot the active carbon of extracting solution mass percent 0.5~0.7% in the extracting solution, stir 5 points Clock, filtering, obtains hippocampus extracting solution;
S3. American Ginseng, fructus lycii and kudzu vine root extract
1) water mentions: weigh American Ginseng, fructus lycii and pueraria lobata according to the parts by weight, then be added water ultrasonic-leaching 30~ 120min, 40 mesh screens obtain filtrate and filter residue, by filter residue repeat above step 1~2 time, by obtained filtrate merge water mentions Liquid;
2) it ferments: Aqueous extracts being accessed into fermentation seed liquid, fermentation obtains fermentation liquid;
S4. other feedstock processings
Weigh rhizoma polygonati, hawthorn, lotus seeds, arillus longan and dried orange peel according to the parts by weight, then be added water ultrasonic-leaching 30~ 120min, 40~80 mesh screens obtain filtrate and filter residue, and filter residue is repeated above step 1~2 time, obtained filtrate is merged Chinese medicine extract;
S5 mixing
Shellfish meat extracting solution, hippocampus extracting solution, fermentation liquid and the Chinese medicine extract that step 1, step 2, step 3 and step 4 are obtained It is mixed evenly, sterilizes, shellfish meat Anti-fatigue drink is made.
4. the production method of shellfish meat Anti-fatigue drink according to claim 3, which is characterized in that the step S1 and step In S2, the compound protease is trypsase and subtilopeptidase A.
5. the production method of shellfish meat Anti-fatigue drink according to claim 4, which is characterized in that the complex enzyme is according to every The calculating of gram homogenate includes: 20~100U/g of 200~500U/g of trypsase and subtilopeptidase A.
6. the production method of shellfish meat Anti-fatigue drink according to claim 3, which is characterized in that in the step S1, institute Stating defishying is 3~4h of deodorant at 4~15 DEG C.
7. the production method of shellfish meat Anti-fatigue drink according to claim 3, which is characterized in that the step S3 and S4 In, the working frequency of the ultrasonic wave is 25~35kHz, ultrasonic intensity is 300~500W, temperature is 50~65 DEG C.
8. the production method of shellfish meat Anti-fatigue drink according to claim 3, which is characterized in that in the step S3, hair It include lactobacillus, Bifidobacterium, acetic acid bacteria and saccharomycete in ferment seed liquor.
9. the production method of shellfish meat Anti-fatigue drink according to claim 3, which is characterized in that in the step S3, hair Ferment condition are as follows: 30~50 DEG C, 5~20d of fermentation period.
CN201910240288.2A 2019-03-28 2019-03-28 A kind of shellfish meat Anti-fatigue drink and preparation method thereof Pending CN109874952A (en)

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Application publication date: 20190614