CN109870448B - Colloidal gold test paper card detection instrument and corresponding detection control method - Google Patents
Colloidal gold test paper card detection instrument and corresponding detection control method Download PDFInfo
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Abstract
The detecting instrument comprises a detecting table, a shooting unit and a detecting control unit, wherein a detecting area is arranged on the detecting table, and the shooting range of the shooting unit at least covers the detecting area; the detection control unit records and analyzes the image signals generated by the camera unit, and 3 or more colloidal gold test paper cards can be placed in the detection area at the same time; and the detection control unit separates each colloidal gold test paper card from the image signal and monitors the detection process of each colloidal gold test paper card. After the colloidal gold test paper card detection instrument and the corresponding detection control method are adopted, the colloidal gold test paper card detection instrument can monitor the detection processes of a plurality of colloidal gold cards at the same time and can automatically judge and read the detection results, can adapt to complex and changeable detection processes, and dynamically completes the detection of samples according to actual scenes.
Description
Technical Field
The invention relates to a colloidal gold test paper card detection instrument and a corresponding detection control method.
Background
Rapid test devices for testing chemical and biological substances are widely cited and often replace some of the traditional laboratory tests. These test devices include various types of tests used to perform such tests as human diagnostics, livestock testing, and food environment testing, among others.
Digital reading devices for testing test devices are also widely used, which are designed to simulate mainly manual diagnosis by means of the naked eye, a microscope or a magnifying glass. Such devices also employ image processing techniques for testing or diagnosis in order to enhance human use. This improvement is achieved by applying other sensitive but realizable techniques.
The colloidal gold method is one of the most widely used test methods at present. The colloidal gold is gold particles with a certain size polymerized by chloroauric acid (HAuCl 4) under the action of reducing agents such as white phosphorus, ascorbic acid, sodium citrate, tannic acid and the like, and becomes a stable colloidal state due to the electrostatic action, so that a negatively charged hydrophobic colloidal solution is formed, and becomes a stable colloidal state due to the electrostatic action, so that the colloidal gold is called as colloidal gold. Colloidal gold is negatively charged in a weak alkaline environment and can form firm combination with positive charge groups of protein molecules, and the biological properties of the protein are not affected because the combination is electrostatic combination. Colloidal gold can bind to many other biological macromolecules in addition to proteins. According to some physical properties of colloidal gold, such as high electron density, particle size, shape and color reaction, and the immunological and biological properties of the conjugate, colloidal gold has been widely used in the fields of immunology, histology, pathology, cell biology, etc. The colloidal gold labeling is a coating process in which a polymer such as a protein is substantially adsorbed to the surface of colloidal gold particles. The adsorption mechanism may be negative charges on the surface of the colloidal gold particles, and the colloidal gold particles and positive charge groups of proteins form firm combination due to electrostatic adsorption. Colloidal gold particles of various particle diameters, that is, different colors, can be conveniently prepared from chloroauric acid by a reduction method. The spherical particles have strong adsorption function to proteins, can be non-covalently combined with staphylococcus protein A, immunoglobulin, toxin, glycoprotein, enzyme, antibiotics, hormone, bovine serum albumin polypeptide conjugate and the like, and therefore, become a very useful tool in basic research and clinical experiments.
The immunogold labeling technique (Immunogold labelling technique) mainly exploits the high electron density properties of gold particles, where gold-labeled proteins bind, black brown particles are visible under a microscope, and when these labels aggregate in large amounts at the corresponding ligands, red or pink spots are visible to the naked eye, thus for qualitative or semi-quantitative rapid immunoassay methods, this reaction can also be amplified by the deposition of silver particles, known as immunogold-silver staining.
FIG. 1 is a schematic diagram of a conventional colloidal gold test strip. The colloidal gold test paper card 1 mainly comprises a sample hole 2 and an observation hole 3, wherein the observation hole 3 is provided with a detection line 5 and a quality control line 4. In addition, the test paper card 1 is also printed with a two-dimensional code area 6 for identifying the identity of the test paper card and part of the information for detection.
The existing colloidal gold detection is carried out through naked eyes or after a colloidal gold card is inserted into an instrument, the colloidal gold detection is simple and convenient, but the detection process cannot be stored, and the detection result is subjective; the latter can be uploaded with the help of instrument, and detection result is also more objective, can be accurate to every to the management of colloidal gold card strip, but need insert the colloidal gold card in the detection process in the instrument, the plug is troublesome, detects the easy instrument of polluting of sample, and the quantity of once detecting card strip is limited moreover, and most is a card or three cards, and detection efficiency is lower.
Disclosure of Invention
The invention aims to provide a colloidal gold test paper card detection instrument and a corresponding detection control method, which can monitor the detection processes of a plurality of colloidal gold cards at the same time, can automatically judge and read the detection results, can adapt to complex and changeable detection processes, and can dynamically finish the detection of samples according to actual scenes.
In order to achieve the above purpose, the invention provides a colloidal gold test paper card detection instrument, which comprises a detection table, a shooting unit and a detection control unit, wherein a detection area is arranged on the detection table, and the shooting range of the shooting unit at least covers the detection area; the detection control unit records and analyzes the image signals generated by the camera unit, and 3 or more colloidal gold test paper cards can be placed in the detection area at the same time; and the detection control unit separates each colloidal gold test paper card from the image signal and monitors the detection process of each colloidal gold test paper card.
Further, the detection control unit determines the detection result of each colloidal gold test paper card through the image signal.
Further, the detection process monitored by the detection control unit comprises operation actions of the colloidal gold test paper card, and the operation actions comprise putting in, taking out and moving the colloidal gold test paper card.
Furthermore, the colloidal gold test paper card comprises a two-dimensional code for recording the identity information of the colloidal gold test paper card, and the detection control unit generates a detection record according to the two-dimensional code and records the detection process, the detection result and the operation action of the colloidal gold test paper card.
The invention also provides a detection control method for the colloidal gold test paper card detection instrument, which comprises the steps of placing the colloidal gold test paper card to be detected on the detection table; recording and analyzing the image signal generated by the image capturing unit; separating each colloidal gold test paper card from the image signal; and monitoring the detection process of each colloidal gold test paper card.
Further, the method further comprises determining a detection result of each colloidal gold test paper card according to the image signals.
Further, the method further comprises the step of identifying the operation action of each colloidal gold test paper card according to the image signals, and executing the operation of detecting and recording the colloidal gold test paper card on software according to the operation action.
Further, the method further comprises the step of identifying the two-dimensional code on the colloidal gold test paper card according to the image signal, and generating a detection record of the colloidal gold test paper card according to the two-dimensional code.
Still further, the method further includes identifying each of the identified colloidal gold test paper cards on the image signal, and a detection process and a detection result of each of the colloidal gold test paper cards, and displaying the identified image signal.
Further, the operation actions include putting in the colloidal gold test paper card, taking out the colloidal gold test paper card and moving the colloidal gold test paper card, wherein the corresponding detection record operation is to add a new detection record, stop or terminate a detection record, and trace and mark the colloidal gold test paper card.
After the colloidal gold test paper card detection instrument and the corresponding detection control method are adopted, the colloidal gold test paper card detection instrument can monitor the detection processes of a plurality of colloidal gold cards at the same time and can automatically judge and read the detection results, can adapt to complex and changeable detection processes, and dynamically completes the detection of samples according to actual scenes.
Drawings
FIG. 1 is a schematic diagram of a conventional colloidal gold test card;
FIG. 2 is a system frame diagram of a colloidal gold reader according to the present invention;
FIG. 3 is a schematic top view of a test station performing a test task;
FIG. 4 is a flow chart of the detection method of the present invention;
fig. 5 is a flowchart of the operation recognition application of the colloidal gold card according to the present invention.
Detailed Description
FIG. 2 is a system frame diagram of the colloidal gold reader of the present invention. The colloidal gold reader comprises a detection table 22, a camera 27 and an LED lamp 26 arranged on the camera. The detection table 22 is provided with a support 23 and a detachable cover 21, wherein the support 23 is fixedly erected on the detection table 22, the upper part of the support 23 is provided with a camera 27 and an LED lamp 26, the detachable cover 21 covers the camera 27, the support 23 and other parts, and forms a closed space together with the detection table 22, so that the temperature and the humidity of the space are kept relatively stable. The camera 27, the LED lamp 26, and the power line and control line of the temperature and humidity sensor provided in the above-mentioned closed space are connected to the control terminal 25 through the cable 24. The control terminal 25 is provided with detection control software which can control the camera 27 and the LED lamp 26 to work, read the image data of the camera 27 and the temperature and humidity data of the temperature and humidity sensor, identify each colloidal gold test paper card placed on the detection table 22 by carrying out image analysis and identification on the image data captured by the camera 27, read the two-dimensional code data on each test paper card and establish corresponding detection records according to the two-dimensional code data; meanwhile, the software starts or ends the corresponding detection record by detecting the placement and the removal of the colloidal gold test paper card.
Fig. 3 is a schematic top view of the inspection station 22 when performing inspection tasks. The detection area on the detection table 22 is divided into 4 sub-areas 29 such as A, B, C and D by a mark line 28, the mark line 28 is used for image recognition assistance and positioning recognition of a colloidal gold test paper card, and a plurality of colloidal gold test paper cards 30 dropping samples are randomly placed in the 4 sub-areas. As can be seen from the figure, a colloidal gold test paper card is respectively placed in each subarea A, C, D, and 3 colloidal gold test paper cards are placed in subarea B.
FIG. 4 is a flow chart of the detection method of the present invention. First, a test paper card to be tested is put into the test stand 22, and then a test process is performed by test control software installed in the control terminal 25. The specific detection process is as follows;
step 40, starting a detection task;
step 41, starting a camera, and carrying out image recognition and analysis on a shot video or photo;
step 42, separating each colloidal gold card from the photographed video or photo;
step 43, identifying the two-dimension code on each colloidal gold card, establishing a detection record for each colloidal gold card according to the two-dimension code information, and monitoring the detection process; in the step, whether a new colloidal gold card is put in or whether a colloidal gold card is taken out is detected, if the new colloidal gold card is put in, a detection record is newly established, and if the new colloidal gold card is taken out, the recording process of the colloidal gold card is stopped or terminated;
step 44, judging whether the detection of the colloidal gold card is finished, if so, executing step 45, otherwise, executing step 43. Two methods for judging the end of the detection of the colloidal gold card are available, one is a detection time method, the detection is ended when the preset detection time is up, and the other is to judge whether the color reaction of the colloidal gold card is stable, and if so, the detection is ended;
step 45, if the detection is finished, the detection control software judges the detection result of the colloidal gold card, generates a detection photo and video for the colloidal gold card, indicates that the card strip is finished in an animation form on the screen of the control terminal 25, and simultaneously displays the detection result, wherein the detection result is determined by analyzing the color changes of the detection line 5 and the quality control line 4 on the colloidal gold test paper card through an image, and the method is widely used in the existing colloidal gold card detection instrument;
step 46, judging whether all the colloidal gold cards placed on the detection table 22 are detected, if not, turning to step 43, and if all the detection is finished, executing step 47;
step 47, the corresponding detection photo, detection video and detection result of each detection record are processed. The detected photo and the detected video contain real image information, and also contain information such as indication marks, animations, detection result display and the like generated in the image recognition and analysis process.
Fig. 5 is a flowchart of an operation recognition application of the colloidal gold card according to the present invention, which includes step 50, recognizing an operation of the colloidal gold card, and step 51, executing a corresponding operation according to the operation. The operation actions of the colloidal gold card on the detecting table 22 include putting in the colloidal gold detecting card, taking out the colloidal gold detecting card, moving the colloidal gold detecting card, etc., and the corresponding operations are to add a detecting record, stop or terminate a detecting record, and trace and mark the colloidal gold detecting card, respectively. Wherein, when detecting that one colloidal gold is taken out but the detection is not finished, stopping the detection processing, and continuing the detection record of the test paper card; if a colloidal gold card is detected to be taken out and the detection of the colloidal gold card is finished, the detection record of the colloidal gold card is terminated.
The two-dimensional code or the hyperlink corresponding to the two-dimensional code on the colloidal gold test paper card at least comprises the following information:
1) The ID number can be an ID number issued by a related department for the test paper card in a unified way;
2) The manufacturer, namely the manufacturer of the test paper card;
3) The production date, namely the production date of the test paper card;
4) The name of the detected substance, which indicates the name of the substance used for detection by the test paper card, such as aflatoxin, H5N1 influenza virus and the like, can also be a code of the detected substance specified according to a certain standard;
5) The detection conditions show conditions such as temperature, humidity and the like when the test paper card is detected, and the colloidal gold card reader can adjust the detection environment according to the conditions;
6) The test result judging curve shows the immunoreaction dynamics curve of the test wire (T wire) and the control wire (C wire) of the colloidal gold test paper card obtained according to the laboratory environment, the color development of the test paper card T, C is continuously deepened along with the time extension, the optical brightness values of the T wire and the C wire are read and respectively recorded as Bt and Bc, and the ratio of Bt/Bc is calculated. The Bt/Bc ratio can be stable in a certain response time, and after the Bt/Bc ratio is stable, the ratio is used as a quantitative analysis result and is used for quantitatively judging the content of the detected object according to the result.
For information and application of the two-dimensional code on the colloidal gold test paper card, see the patent authorized by the applicant: CN201310227223.7, a colloidal gold test paper card, a corresponding colloidal gold analyzer and a testing method.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and those skilled in the art may make modifications or equivalent substitutions without departing from the spirit and scope of the present invention, and it should be covered in the claims of the present invention.
Claims (9)
1. The detecting instrument is characterized by comprising a detecting table, a shooting unit and a detecting control unit, wherein a detecting area is arranged on the detecting table, the shooting range of the shooting unit at least covers the detecting area, the detecting area supports the simultaneous placement of 3 or more than 3 colloidal gold test cards, and the colloidal gold test cards comprise two-dimensional codes for recording identity information of the colloidal gold test cards; the detection control unit records and analyzes the image signals generated by the image pickup unit, separates the image of each colloidal gold test paper card from the image signals, utilizes the image of each colloidal gold test paper card to identify the two-dimensional code on each colloidal gold test paper card, establishes detection records for each colloidal gold test paper card according to the information of the two-dimensional code, and monitors the detection process of each colloidal gold test paper card through the image signal identification, wherein the detection process comprises the operation actions of the colloidal gold test paper card, the operation actions comprise the putting in, taking out and moving of the colloidal gold test paper card, the putting in operation actions correspond to newly adding one detection record, and the taking out operation actions correspond to stopping or stopping one detection record.
2. The colloidal gold test strip card testing instrument as set forth in claim 1, wherein the testing control unit determines the testing result of each colloidal gold test strip card by the image signal.
3. The colloidal gold test strip card testing apparatus as set forth in claim 2, wherein the test record records the test process, the test result and the operation action of the colloidal gold test strip card.
4. A detection control method for the colloidal gold test strip card detection instrument according to any one of claims 1 to 3, characterized in that the method comprises placing 3 or more colloidal gold test strips to be detected on the detection table; recording and analyzing the image signal generated by the image capturing unit; separating the image of each colloidal gold test paper card from the image signal; and monitoring the detection process of each colloidal gold test paper card by using the image of the colloidal gold test paper card.
5. The method of claim 4, further comprising determining a detection result of each of the colloidal gold test cards according to the image signal.
6. The method of claim 5, further comprising identifying an operation of each of the colloidal gold test paper cards according to the image signal, and executing an operation of detecting a record of the colloidal gold test paper card on software according to the operation.
7. The method of claim 6, further comprising identifying a two-dimensional code on the colloidal gold test paper card according to the image signal, and generating a detection record of the colloidal gold test paper card according to the two-dimensional code.
8. The method of claim 7, further comprising identifying each of the identified colloidal gold test paper cards on the image signal, and displaying the identified image signal according to a detection process and a detection result of each of the colloidal gold test paper cards.
9. The method according to any one of claims 6 to 8, wherein the operation includes inserting a colloidal gold test paper card, taking out the colloidal gold test paper card and moving the colloidal gold test paper card, and the corresponding detection record operation is to add a detection record, stop or terminate a detection record, and trace and mark the colloidal gold test paper card.
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| CN110780064B (en) | 2019-11-15 | 2020-10-16 | 中国农业科学院农业质量标准与检测技术研究所 | Method and system for detecting target component by using mobile terminal |
| CN113610205B (en) * | 2021-07-15 | 2022-12-27 | 深圳宇晰科技有限公司 | Two-dimensional code generation method and device based on machine vision and storage medium |
| CN113624763A (en) * | 2021-10-13 | 2021-11-09 | 深圳市帝迈生物技术有限公司 | Sample analysis device, monitoring method thereof and computer-readable storage medium |
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