CN103995108A - Colloidal gold detection system and corresponding colloidal gold chart reader - Google Patents

Colloidal gold detection system and corresponding colloidal gold chart reader Download PDF

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CN103995108A
CN103995108A CN201410210890.9A CN201410210890A CN103995108A CN 103995108 A CN103995108 A CN 103995108A CN 201410210890 A CN201410210890 A CN 201410210890A CN 103995108 A CN103995108 A CN 103995108A
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collaurum
test item
chart
chart scanner
scanner
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CN103995108B (en
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万克家
李乾
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Sharp Instrument Science And Technology Nantong Co Ltd That Reaches Of Intelligence
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Sharp Instrument Science And Technology Nantong Co Ltd That Reaches Of Intelligence
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor

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Abstract

The invention discloses a colloidal gold detection system and a corresponding colloidal gold chart reader. The system comprises a plurality of colloidal gold chart readers and a detection item download server, wherein the detection item download server is used for pushing corresponding detection items to the colloidal gold chart readers according to the colloidal gold chart readers; the colloidal gold chart readers are used for displaying the pushed detection items. By adopting the system and the chart reader, networking and informatization of sections such as production, detection and sale of a colloidal gold test paper chart and the colloidal gold chart reader are achieved, and the applicability of the colloidal gold chart reader is improved.

Description

A kind of collaurum detection system and corresponding collaurum chart scanner
Technical field
The present invention relates to a kind of collaurum detection system and corresponding collaurum chart scanner.
Background technology
Device for quick testing for test chemistry and biological substance is extensively quoted, and often replaces the test in some traditional laboratories.These proving installations comprise polytype test, are used to carry out such as mankind's diagnosis, herding test and food product environment test etc.
The digital fetch equipment that is used for testting proving installation is also widely used, and they are mainly to imitate the mode of diagnosing by craft such as bore hole, microscope or magnifieres to be designed to form.This equipment is tested or diagnoses with also having applied image processing technique in order to improve the mankind.This raising is the technology by having applied the sensitive of other but can having realized.
Colloidal gold method is to use at present the widest a kind of method of testing.Collaurum be by gold chloride (HAuCl4) reductive agent as the effect such as white phosphorus, ascorbic acid, sodium citrate, tannic acid under, can be grouped to a certain size gold grain, and because electrostatic interaction becomes a kind of stable colloidal state, form electronegative hydrophobic sol solution, because electrostatic interaction becomes stable colloidal state, therefore claim collaurum.Collaurum is electronegative under weak base environment, can form firmly and be combined with the positive charge group of protein molecule, because this combination is electrostatical binding, so do not affect the biological nature of protein.Collaurum except with protein bound, can also be combined with many other biomacromolecules.According to some physical behaviors of collaurum, as high electron density, grain size, shape and color reaction, add immunity and the biological characteristics of bond, thereby make collaurum be widely used in the fields such as immunology, histology, pathology and cell biology.Colloid gold label is in fact the coated process that the macromolecules such as protein are adsorbed to colloid gold particle surface.Adsorption mechanism may be colloid gold particle surface negative charge, forms strong bonded with the positive charge group of protein because of Electrostatic Absorption.By reducing process, can be easily from gold chloride, prepare various different-grain diameters, the colloid gold particle of different colours namely.This spherical particle has very strong adsorption function to protein, can with the non-covalent combinations such as staphylococcal protein A, immunoglobulin (Ig), toxin, glycoprotein, enzyme, microbiotic, hormone, bovine serum albumin(BSA) polypeptide conjugate, thereby in fundamental research and clinical trial, become very useful instrument.
Immuno-gold labeling technology (Immunogold labelling technique) has mainly utilized gold grain to have the characteristic of high electron density, at gold mark protein combination place, visible pitchy particle under the microscope, when these labels are assembled in a large number at corresponding part place, naked eyes red color visible or pink spot, thereby for qualitative or semiquantitative tachysynthesis detection method, this reaction also can be exaggerated by the deposition of silver-colored particle, is referred to as immunogold silver staining.
Fig. 1 is a kind of colloidal gold test paper card schematic diagram, in published application for a patent for invention number, is open in " colloidal gold test paper card, corresponding collaurum analyser and the method for testing " in " 201310227223.7 ".This colloidal gold test paper card 1 mainly comprises sample well 2 and viewport 3, and viewport 3 is provided with detection line 5 and nature controlling line 4.With the colloidal gold test paper card difference shown in Fig. 1, on test card 1, be also printed on the Quick Response Code region 6 of detecting the information of using for identifying test card identity and part.The information of test card comprises that test card identity information and test card detect information.Wherein, test card identity information comprises:
1) No. ID, test card, and it can be that relevant departments are No. ID that test card unification is issued;
2) manufacturer, the i.e. manufacturer of this test card;
3) date of manufacture, the i.e. date of manufacture of this test card;
4) test item ID, this is unique identifier of test item, and different test items has different test item ID, and it can show that this test card is used for the material title detecting, as aflatoxins, H5N1 influenza virus etc.
Test card detection information comprises:
1) testing conditions, the conditions such as temperature when it shows this test card detection, humidity, collaurum chart scanner of the present invention can regulate testing environment according to these conditions;
2) testing result judgment curves, show this colloidal gold test paper card detection line (T line), control line (C line) the immune response kinetic curve that according to laboratory environment, obtain, test card T, C colour developing extends in time color and constantly deepens, read the optical brightness value of T line and C line and be designated as respectively Bt and Bc, calculating the ratio of its Bt/Bc.Bt/Bc ratio can tend towards stability within certain response time, wait stable after, using ratio as quantitative analysis results, and according to this result for the quantitative content of judgement detected material.
Along with the development of internet, a lot of industries is all shifting to internet." a kind of information-pushing method and Information Push Server " that application number is " 201010222615.0 " discloses a kind of information-pushing method and Information Push Server, main contents comprise: by Information Push Server, the first website and the second website are associated together, without first user, on the second website, carry out keyword search, by server, according to the merchandise news of first user issue, determine that first user wishes to obtain after the commodity classification of commodity, from be registered in the user of the second website, inquire the merchandise news that the second user of identical goods classification and/or this second user issue can be provided, avoided causing because key word is inaccurate the inaccurate problem of Search Results of first user, make the second user who finally inquires can provide first user needed commodity.
Application number discloses a kind of advertisement pushing system and advertisement pushing equipment, Advertisement Server for " a kind of advertisement pushing system and advertisement pushing equipment, the Advertisement Server " of " 201310012728.1 ", and advertisement pushing system comprises client, web page server, advertisement pushing equipment, advertising strategy decision device and Advertisement Server; Advertisement pushing equipment receives after advertisement pushing strategy, sends the redirection message of the IP that carries client according to advertisement pushing strategy to client; Client sends the advertisement pattern request of the IP address of carrying client to Advertisement Server according to redirection message; Advertisement Server obtains behind the IP address, source of advertisement pattern request and the IP address of the client of carrying; Determine the IP address, source of advertisement pattern request when identical with the IP address of the client of carrying, to IP address, source, sending the first advertisement pattern template that comprises Webpage framework and advertisement page framework; Ad content and web page contents that client receives according to advertisement pattern template for displaying.By the way, the present invention can improve the success ratio of advertisement pushing.
Although it is very ripe that Internet industry has developed, but collaurum chart scanner belongs to industry equipment, just in production and processing departments such as some scientific research institutions, law enforcement agency of government and food, medicine, herding, cultivation, use, range of application is narrower, reagent manufacturer researches and develops production separately accordingly, produce to exist with the research and development of collaurum detector and do not mate, cause research and development production and the marketing activity of reagent, colloid gold card, collaurum chart scanner to have gap, the correlation technique of internet can not be applied to collaurum upstream and downstream industry.
Summary of the invention
The object of this invention is to provide a kind of collaurum detection system and corresponding collaurum chart scanner, adopt after this system and chart scanner, can realize the production and sales networking of colloidal gold test paper card and collaurum chart scanner, informationization, the adaptability of raising collaurum chart scanner.
To achieve these goals, the invention provides a kind of collaurum detection system, described system comprises a plurality of collaurum chart scanners, and test item Download Server, described test item Download Server pushes correspondingly test item according to described collaurum chart scanner and arrives described collaurum chart scanner, and described collaurum chart scanner shows the test item of described propelling movement.
Further, the test item of described propelling movement is the support of described collaurum chart scanner but is not configured in the test item of described collaurum chart scanner.
Further, described system also comprises colloid gold card Market Information Service device, for the market information of storing colloid gold card, described colloid gold card Market Information Service device is according to the test item of described collaurum chart scanner and configuration thereof, push the market information of corresponding colloid gold card to described collaurum chart scanner, described collaurum chart scanner shows the market information of the colloid gold card of described propelling movement.
Further, described system also comprises testing result storage server, for storing testing process information and the net result information of described collaurum chart scanner.
Further, described test item Download Server canned data comprises, test item title, test item ID, test item information and test item control module; Described colloid gold card Market Information Service device canned data comprises test card identity information, the collaurum chart scanner information that test card adapts to and the market information of test card.
The present invention also provides a kind of collaurum chart scanner, and described chart scanner comprises, detecting unit, for carrying out the test item of setting; Network communication unit, for uploading or download described chart scanner testing desired data; Display unit, for showing testing process and the detection information of described chart scanner, described chart scanner also comprises test item download management unit, and described test item download management unit obtains described chart scanner support by described network communication unit but is not configured in the test item of described chart scanner.
Further, described chart scanner also comprises colloid gold card market information administrative unit, and described colloid gold card market information administrative unit, according to the test item of described chart scanner and setting, is obtained colloid gold card market information by described network communication unit.
Further, described chart scanner also comprises colloid gold card market information administrative unit, described colloid gold card market information administrative unit is according to described chart scanner and insert the 2 D code information of the colloid gold card of described chart scanner, by described network communication unit, obtains colloid gold card market information.
Adopt after this system and chart scanner, can realize the networking of the links such as production, detection, sale of colloidal gold test paper card and collaurum chart scanner, informationization, improves the adaptability of collaurum chart scanner.
Accompanying drawing explanation
Fig. 1 is a kind of colloidal gold test paper card schematic diagram;
Fig. 2 is the system framework schematic diagram of collaurum chart scanner of the present invention;
Fig. 3 is system framework figure of the present invention;
Fig. 4 is the test item download management operation interface of this software package;
Fig. 5 is the process flow diagram that test item is downloaded in the analysis of collaurum chart scanner;
Fig. 6 is another kind of implementation method of the present invention;
Fig. 7 provides interface for colloid gold card information provision;
Fig. 8 colloid gold card information provision processing flow chart.
Embodiment
Fig. 2 is the system framework schematic diagram of collaurum chart scanner of the present invention.Collaurum chart scanner 20 comprises main control unit 21, for the normal work of control instrument; Screen display unit 22 is for showing test process and test result; Graphics processing unit 23 is for carrying out analyzing and processing to the captured result of optical unit 26; Optical unit 26 is for carrying out optical detection to putting into the colloidal gold test paper card 25 of test section 24, graphics processing unit 23 obtains the optical brightness value of colloidal gold test paper card detection line and nature controlling line, wherein needs to remove through background the background that module 30 is removed detection line and nature controlling line; Main control unit 21 is determined testing result by calculating the ratio of detection line optical brightness value and nature controlling line optical brightness value; Test section temperature humidity control module 27 is for controlling the humidity and temperature of test section 24; Network communication unit 28 is for uploading or Download Info.Storage unit 29 is for storing corresponding data, and as the photo of taking, test process, test result etc., all data of uploading through network communication unit 28 can first be stored in here.Lighting unit 31, for being thrown light in test section 24, when optical unit 26 carries out optical detection, starts lighting unit 31, makes the clear picture of taking.Optical unit 26 can also obtain the Quick Response Code data of colloidal gold test paper card.
Fig. 3 is system framework figure of the present invention.Desk-top collaurum chart scanner 53 and portable colloidal gold chart scanner 54 be by internet 55 and server 50,51, and 52 carry out communication, and wherein, server 50 is test item Download Server, for providing test item control module to collaurum chart scanner; Server 51 is testing result storage server, and for receiving the testing result of storage collaurum chart scanner, testing result comprises intermediate data in testing process and the net result of detection; Server 52 is for detecting information server, for storing the information provision of colloid gold card.Server 50,51 and 52 can separate physically, also can be together, and even in a database.
In order to facilitate the download of test item, the various test item information of test item Download Server 50 storage, wherein information comprises:
1) test item title, as aflatoxins, H5N1 influenza virus etc.;
2) test item ID, this is unique identifier of test item, different test items has different test item ID;
3) test item information, information comprises the simple introduction of Detection of content and the Detection of content of this test item;
4) test item control module, this module can be downloaded in collaurum chart scanner and by collaurum, be detected analysis software and call, and completes corresponding test item.
In order to facilitate uploading of testing result, testing result storage server 51 storage testing process and net results, wherein information comprises:
5) test item information, comprises test item ID, detection time, detection site, testing staff etc.;
6) collaurum chart scanner information, comprises numbering, model, producer's information of collaurum chart scanner etc.;
7) test card identity information, comprising: No. ID, it can be that relevant departments are No. ID that test card unification is issued; Manufacturer, the i.e. manufacturer of this test card; Date of manufacture, the i.e. date of manufacture of this test card; Test item ID, this is unique identifier of test item, and different test items has different test item ID, and it can show that this test card is used for the material title detecting, as aflatoxins, H5N1 influenza virus etc.;
8) detect pilot process information, as the photo of 26 BRs of optical unit;
9) detect net result, if main control unit 21 is by calculating the definite testing result of ratio of detection line optical brightness value and nature controlling line optical brightness value.
For convenient, detect, the supply-demand information of colloid gold card Market Information Service device 52 storage colloid gold card, wherein information comprises:
10) test card identity information, comprising: No. ID, it can be that relevant departments are No. ID that test card unification is issued; Manufacturer, the i.e. manufacturer of this test card; Date of manufacture, the i.e. date of manufacture of this test card; Test item ID, this is unique identifier of test item, and different test items has different test item ID, and it can show that this test card is used for the material title detecting, as aflatoxins, H5N1 influenza virus etc.;
11) the collaurum chart scanner information that test card adapts to, comprises the model, producer's information of collaurum chart scanner etc.;
12) market information of test card, comprises vendor name, address, stockpile number and contact method etc.
The system framework figure of desk-top collaurum chart scanner 53 and portable colloidal gold chart scanner 54 as shown in Figure 2, before described its composition and working principle in detail.Desk-top collaurum chart scanner 53 of the present invention and portable colloidal gold chart scanner 54 adopt ANDROID operating system, adopt touch-screen as input block, and collaurum is installed and is detected analysis software package, the test item download management operation interface that Fig. 4 is this software package.Screen 22 comprises existing test item 2201, interface display has test item A 2202, test item B 2203 and test item C 2204, can comprise test item 1 2211, test item 2 2212, test item 3 ~ 9 2213 and test item 10 2214 for downloading test item 2201.
Can be in advance in collaurum chart scanner built-in or by network, download the test item of all supports, and store following information:
1) test item title;
2) test item ID, this is unique identifier of test item, different test items has different test item ID;
3) test item information, information comprises the simple introduction of Detection of content and the Detection of content of this test item.
When collaurum chart scanner starts, first collaurum detection analysis software is analyzed and is read the test item having had, and shows the test item having had on screen, then analyzes and obtains the test item not having in collaurum chart scanner, and show on screen, for user, select to download.
Fig. 5 is the process flow diagram that test item is downloaded in the analysis of collaurum chart scanner.First, step 61 is obtained the test item not having in collaurum chart scanner; Step 62 is selected a test item; Step 63 is downloaded the control module of this test item from outside; Step 64 starts this test item, utilizes the control module of downloading to complete whole testing process.In step 61, the test item not having derives from Download Server 50, Download Server 50 according to the model of collaurum chart scanner with and the test item that had analyze the test item that it does not have.In step 63, the control module of test item also derives from Download Server 50.When detection is carried out, the intermediate data in testing process is uploaded to testing result storage server 51, after detection completes, testing result is uploaded to testing result storage server 51.
Fig. 6 is another kind of implementation method of the present invention.Step 71, test item Download Server 50 obtains the type of existing test item in collaurum chart scanner and this chart scanner; Step 72, the test item not having that is applicable to this collaurum chart scanner of test item Download Server 50 screenings; Step 73, the test item information that Download Server 50 transmissions filter out is to this collaurum chart scanner; Step 74, collaurum chart scanner shows the test item filtering out; Step 75, user selects the test item needing, and buys and download the control module of this test item; Step 76, starts this test item.In step 75, the control module of test item also derives from Download Server 50.When detection is carried out, the intermediate data in testing process is uploaded to testing result storage server 51, after detection completes, testing result is uploaded to testing result storage server 51.
For service is better provided, Fig. 7 provides interface for colloid gold card information provision.Screen 22 comprises existing test item 2201, interface display has test item A 2202, test item B 2203 and test item C 2204, select existing test item A 2202, colloid gold card information provision 2220 shows current colloid gold card 1 2221, colloid gold card 2 2222, colloid gold card 3 ~ 9 2223 and colloid gold card 10 2224 for buying.
Fig. 8 colloid gold card information provision processing flow chart.First, step 81 is selected an existing test item from collaurum chart scanner; Step 82, obtains the collaurum card information of adaptation according to the model of collaurum chart scanner and test item; Step 83, shows the collaurum card information obtaining; Step 84, selects to buy corresponding colloid gold card.In step 82, collaurum card information derives from colloid gold card Market Information Service device 52.
In addition, in step 82, the test item information that on the model that colloid gold card information provision treatment scheme can also be by chart scanner and colloid gold card, 2 D code information is recorded completes.
Finally it should be noted that: above embodiment is the unrestricted technical scheme of the present invention in order to explanation only; those of ordinary skill in the art can modify or be equal to replacement the present invention; and not departing from any modification or partial replacement of the spirit and scope of the present invention, it all should be encompassed in claim protection domain of the present invention.

Claims (8)

1. a collaurum detection system, it is characterized in that, described system comprises a plurality of collaurum chart scanners, and test item Download Server, described test item Download Server pushes correspondingly test item according to described collaurum chart scanner and arrives described collaurum chart scanner, and described collaurum chart scanner shows the test item of described propelling movement.
2. collaurum detection system as claimed in claim 1, is characterized in that, the test item of described propelling movement is the support of described collaurum chart scanner but is not configured in the test item of described collaurum chart scanner.
3. the collaurum detection system as described in any one in claim 1 to 2, it is characterized in that, described system also comprises colloid gold card Market Information Service device, for the market information of storing colloid gold card, described colloid gold card Market Information Service device is according to the test item of described collaurum chart scanner and configuration thereof, push the market information of corresponding colloid gold card to described collaurum chart scanner, described collaurum chart scanner shows the market information of the colloid gold card of described propelling movement.
4. collaurum detection system as claimed in claim 3, is characterized in that, described system also comprises testing result storage server, for storing testing process information and the net result information of described collaurum chart scanner.
5. collaurum detection system as claimed in claim 4, is characterized in that, described test item Download Server canned data comprises, test item title, test item ID, test item information and test item control module; Described colloid gold card Market Information Service device canned data comprises test card identity information, the collaurum chart scanner information that test card adapts to and the market information of test card.
6. a collaurum chart scanner, described chart scanner comprises, detecting unit, for carrying out the test item of setting; Network communication unit, for uploading or download described chart scanner testing desired data; Display unit, for showing testing process and the detection information of described chart scanner, it is characterized in that, described chart scanner also comprises test item download management unit, and described test item download management unit obtains described chart scanner support by described network communication unit but is not configured in the test item of described chart scanner.
7. collaurum chart scanner as claimed in claim 6, it is characterized in that, described chart scanner also comprises colloid gold card market information administrative unit, described colloid gold card market information administrative unit, according to the test item of described chart scanner and setting, is obtained colloid gold card market information by described network communication unit.
8. collaurum chart scanner as claimed in claim 6, it is characterized in that, described chart scanner also comprises colloid gold card market information administrative unit, described colloid gold card market information administrative unit is according to described chart scanner and insert the 2 D code information of the colloid gold card of described chart scanner, by described network communication unit, obtains colloid gold card market information.
CN201410210890.9A 2014-05-19 2014-05-19 A kind of collaurum detection system and corresponding colloidal gold chart reader Active CN103995108B (en)

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CN109870448A (en) * 2019-02-09 2019-06-11 智锐达仪器科技南通有限公司 A kind of colloidal gold test paper card detecting instrument and control method is detected accordingly
CN110161224A (en) * 2019-04-29 2019-08-23 成都莱孚科技有限责任公司 A kind of pet disease detecting system based on cloud data processing
CN110164532A (en) * 2019-04-29 2019-08-23 成都莱孚科技有限责任公司 A kind of pet disease test paper detection system with voice broadcast function
CN110164533A (en) * 2019-04-29 2019-08-23 成都莱孚科技有限责任公司 A kind of test paper system for tracing and managing for pet disease detection

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CN110164533A (en) * 2019-04-29 2019-08-23 成都莱孚科技有限责任公司 A kind of test paper system for tracing and managing for pet disease detection
CN110164533B (en) * 2019-04-29 2021-11-09 成都莱孚科技有限责任公司 Test paper tracing management system for pet disease detection

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