CN109810893B - Portable continuous automatic preparation device and method for agar culture medium containing temperature-sensitive active substances - Google Patents
Portable continuous automatic preparation device and method for agar culture medium containing temperature-sensitive active substances Download PDFInfo
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- CN109810893B CN109810893B CN201910162220.7A CN201910162220A CN109810893B CN 109810893 B CN109810893 B CN 109810893B CN 201910162220 A CN201910162220 A CN 201910162220A CN 109810893 B CN109810893 B CN 109810893B
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- 229920001817 Agar Polymers 0.000 title claims abstract description 38
- 239000008272 agar Substances 0.000 title claims abstract description 38
- 239000001963 growth medium Substances 0.000 title claims abstract description 28
- 239000013543 active substance Substances 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 41
- 238000003756 stirring Methods 0.000 claims abstract description 33
- 230000001954 sterilising effect Effects 0.000 claims abstract description 28
- 238000003860 storage Methods 0.000 claims abstract description 15
- 239000007788 liquid Substances 0.000 claims description 79
- 239000000463 material Substances 0.000 claims description 16
- 230000002572 peristaltic effect Effects 0.000 claims description 13
- 239000000126 substance Substances 0.000 claims description 12
- 238000005485 electric heating Methods 0.000 claims description 11
- 230000033001 locomotion Effects 0.000 claims description 11
- 238000004321 preservation Methods 0.000 claims description 9
- 238000003825 pressing Methods 0.000 claims description 9
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 8
- 239000011521 glass Substances 0.000 claims description 7
- 238000004140 cleaning Methods 0.000 claims description 4
- 229910052742 iron Inorganic materials 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 2
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- 239000012528 membrane Substances 0.000 claims 1
- 238000005192 partition Methods 0.000 description 12
- 238000002474 experimental method Methods 0.000 description 5
- 238000011068 loading method Methods 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 2
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Abstract
The invention discloses a portable continuous automatic preparation device and method for agar culture medium containing temperature-sensitive active substances, comprising a shell heat-preserving supporting unit, a multi-specification culture dish fixing pushing unit, an electric control unit, a constant-temperature storage stirring unit, an intermittent rotating unit, an automatic continuous pouring unit and a disinfection and sterilization unit.
Description
Technical Field
The invention relates to the field of biological or medical experimental equipment, in particular to a device and a method for continuously and automatically preparing a portable agar culture medium containing temperature-sensitive active substances.
Background
The preparation of the agar plate culture medium commonly used in medical and biological experiments needs the steps of weighing, pressure steam sterilization, pouring, split charging and the like. The activity of the temperature sensitive substances is easily damaged when the temperature is too high, agar is solidified when the temperature is low, most of agar starts to solidify at about 40 ℃, and the solidified agar needs to be heated to 95 ℃ again to start melting, so that certain special agar culture media, antibacterial experiments and the like often need to add substances sensitive to heat such as blood, serum, antibiotics, indicator bacteria and the like into constant-temperature agar at about 45 ℃ to prepare the agar culture media containing the temperature sensitive substances, and the temperature needs to be strictly controlled in the preparation process. In addition, in order to require uniformity of the experiment, the quantification of the active substance must be ensured, and the agar plates must be prepared by quantitatively pouring agar. Therefore, the agar medium containing the temperature-sensitive active substance needs to ensure the conditions of constant temperature, sterility, quantification and the like in the operation process.
The agar plates sold in the market at present are mostly mass produced by large-scale equipment and do not contain temperature-sensitive substances or only contain common types and content substances, and are easy to deteriorate due to the short shelf life of the temperature-sensitive substances in the transportation process, and are best prepared and used at present, so that the requirements of individual experiments of adding temperature-sensitive substances of different types and content in a laboratory are difficult to meet. The problems existing in the prior art are mainly as follows:
(1) The industrial large-scale culture medium preparation equipment is large in production batch, but the produced culture medium is few in variety, and the requirement of the laboratory on variety and content requirement of temperature-sensitive substances due to variety of experimental samples cannot be met.
(2) The small-sized preparation equipment cannot realize continuous automatic pouring, and a plurality of plates are not fixed simultaneously and the size of the plate fixing device is adjustable, so that the working efficiency is low.
Therefore, development of a device and a method for continuously and automatically preparing a portable agar culture medium containing temperature-sensitive active substances, which are suitable for laboratory researches, is needed.
Disclosure of Invention
Aiming at the problems, the invention provides a device and a method for continuously and automatically preparing the portable agar culture medium containing the temperature-sensitive active substance, which have the advantages of low manufacturing cost, complete functions, simple and reliable operation and good uniformity.
In order to achieve the above purpose, the present invention provides the following technical solutions: a portable continuous automatic preparation device for agar culture medium containing temperature-sensitive active substances comprises: the shell keeps warm supporting element, the fixed propelling movement unit of many specifications culture dish, automatically controlled unit, constant temperature storage stirring unit, intermittent type rotary unit, automatic continuous pouring unit, disinfection and sterilization unit seven units, its characterized in that: the shell heat-insulating support unit is made of heat-insulating light materials, a push guide rail of the multi-specification culture dish fixing push unit is connected with the left shell and the right shell, the electric control unit is connected with the constant-temperature storage stirring unit, the intermittent rotation unit, the automatic continuous pouring unit and the disinfection and sterilization unit through control circuits and supplies current, the constant-temperature storage stirring unit is connected with the automatic continuous pouring unit through a liquid inlet pipeline and conveys culture fluid, the intermittent rotation unit enables a rotating disc of the multi-specification culture dish fixing push unit to synchronously rotate through an output shaft, the intermittent rotation unit and the automatic continuous pouring unit are installed on the lower shell of the shell heat-insulating support unit through brackets, and the disinfection and sterilization unit is installed on the lower portion of the upper shell of the shell heat-insulating support unit through brackets.
Preferably, the shell heat-insulating support unit comprises a front shell, a rear shell, a left shell, a right shell, an upper shell and a lower shell, wherein the front shell, the rear shell, the left shell, the right shell, the upper shell and the lower shell are made of heat-insulating light materials, the front shell is provided with a pushing port, the pushing port can allow pushing of the multi-specification culture dish fixing pushing unit, and the rear shell is connected with other shells through bolts.
Preferably, the fixed pushing unit of many specifications culture dish includes lift holding ring, spring, rotation disc, propelling movement square dish, propelling movement guide rail, positioning magnet, propelling movement guide rail bilateral symmetry installs on left casing, right casing, bilateral symmetry installs positioning magnet on the propelling movement guide rail, and square groove has been opened to propelling movement square dish bottom surface symmetry axis position, and left and right sides symmetry is opened there is the square groove, and the rear that does not open the groove covers there is the iron sheet, has the circular slot above opening, and the center has the round hole, and rotation disc bottom surface diameter position is opened there is the square groove, and the center part is opened there is the square groove that is deeper than former square groove 5mm, has four circular slots above, and three stand of rule distribution in the circular slot, and every round has three spring, and spring one pot head is on the stand in the circular slot, and another pot head is on the bump of lift holding ring, and the concentric clearance of equidimension lift holding ring is installed in the circular slot, and lift holding ring external diameter is set to be greater than the normal specification flat size 2mm.
Preferably, the electric control unit comprises a control circuit, a control panel, a control box and a display screen, wherein the display screen and the control panel are arranged on the front shell, and the control panel transmits signals among the control circuit, the control box, the display screen, the electric heating plate, the temperature sensor, the electric stirrer, the three-phase asynchronous motor, the pouring motor and the ultraviolet lamp.
Preferably, the constant temperature storage stirring unit comprises a liquid loading tank, a heat preservation barrel, an electric heating plate, a partition plate, a temperature sensor and an electric stirrer, wherein water bath heating is adopted, the heat preservation barrel is made of heat preservation and insulation light materials, the partition plate is arranged in the heat preservation barrel, the electric heating plate and the temperature sensor are arranged at the lower part of the partition plate, through holes are formed in the partition plate, the liquid loading tank is arranged at the upper part of the partition plate, the liquid loading tank is detachable and made of high-temperature and high-pressure resistant glass materials, the liquid loading tank is arranged on the partition plate, a vent hole, a liquid outlet and a stirring hole are formed in a tank cover, a bacterial filtering film is arranged in the vent hole, a liquid inlet pipeline is arranged in the liquid outlet, and the electric stirrer is arranged in the stirring hole.
Preferably, the intermittent rotation unit comprises a three-phase asynchronous motor, a driving shaft, a driving wheel, a rotating arm, a rotating rod, a grooved wheel and an output shaft, wherein the three-phase asynchronous motor is connected with the driving shaft through a coupler, the driving shaft is connected with the driving wheel through a key, the driving wheel is in interference fit with the rotating arm, the rotating rod is connected in a shaft hole of the rotating arm through a key, the rotating arm is in matched motion with the grooved wheel, the output shaft is connected with the grooved wheel through a key, the upper end of the output shaft is square, and the rotating disc is driven to periodically and intermittently rotate under the control of the electronic control unit.
Preferably, the automatic continuous pouring unit comprises a pouring motor, a peristaltic pump, a liquid inlet pipeline and a liquid outlet pipeline, wherein the pouring motor controls the peristaltic pump to pump liquid and discharge liquid, the liquid inlet pipeline and the liquid outlet pipeline are respectively arranged at a liquid inlet and a liquid outlet of the peristaltic pump, and the liquid inlet pipeline and the liquid outlet pipeline are detachable and made of high-temperature and high-pressure resistant glass materials.
Preferably, the sterilization unit includes an ultraviolet lamp which is in a closed state after the start of pouring.
A continuous automatic preparation method of a portable agar culture medium containing temperature-sensitive active substances is characterized by comprising the following steps:
s1: opening the rear shell, and installing the peristaltic pump, the liquid inlet pipeline and the liquid outlet pipeline which are subjected to cleaning, disinfection, sterilization and drying treatment into equipment in a sterile environment;
s2: pressing a temperature control key, starting a temperature control system of the equipment to work, and automatically adjusting the temperature of the electric heating sheet by the temperature control system through a temperature sensor after the temperature displayed on the display screen reaches the set temperature so that the system keeps constant temperature all the time;
s3: installing a liquid containing tank filled with agar solution after pressure steam sterilization, adding a temperature-sensitive substance from a liquid outlet when the temperature is cooled to be constant to a required constant temperature, connecting a liquid inlet pipeline, pressing a stirring and sterilizing key on a control panel, starting to automatically stir the solution in the liquid containing tank and simultaneously starting to operate an ultraviolet lamp, and stopping stirring automatically when the set stirring and sterilizing time is reached, and closing the ultraviolet lamp simultaneously;
s4: placing the sterilized and dried plate on a rotating disc to fix in four circular grooves formed in the rotating disc, matching the rotating disc with a pushing square disc, namely aligning the axes of square grooves formed in the lower surface, and inserting square grooves formed in the left side surface and the right side surface of the pushing square disc into working positions along a pushing guide rail to contact with a positioning magnet;
s5: and pressing a pouring button to automatically and continuously pour the agar culture medium solution containing the temperature-sensitive active substance. When the set pouring time is reached, the automatic stop work is finished, namely the pouring is finished, the pushing square plate is pulled out, and the poured culture dish is replaced, so that the pouring can be repeated.
Compared with the prior art, the invention has the beneficial effects that:
(1) Portable small and exquisite, convenient operation. The invention is small and portable, has good flexibility and is suitable for preparing agar culture mediums which are required by individual experiments of different types and contents and contain temperature-sensitive active substances in a laboratory while meeting the preparation conditions of special culture mediums.
(2) Continuous automatic pouring. The agar culture medium device is provided with the plate fixing device which can fix a plurality of plates simultaneously and can adjust the sizes of the plates, can pour the plates continuously and automatically, and can prepare agar culture mediums of any kind and specification theoretically.
(3) The equipment is convenient to disassemble, clean and sterilize. Parts of the liquid loading tank, the liquid inlet pipeline, the liquid outlet pipeline and the like which are contacted with the culture medium solution are designed in a detachable mode, and the materials are high-temperature and high-pressure resistant glass, so that the disassembly, cleaning and sterilization are convenient.
Drawings
FIG. 1 is a schematic view of the present invention
FIG. 2 is a schematic diagram of each unit of the present invention
FIG. 3 is a schematic view of the outer shell of the present invention
FIG. 4 is a schematic view of the components of the constant temperature storage stirring unit of the present invention
FIG. 5 is a schematic diagram of parts of a multi-format culture dish fixing and pushing unit according to the present invention
FIG. 6 is a schematic view of the components of the present invention
In fig. 2: the device comprises a shell heat-insulating supporting unit 1, a multi-specification culture dish fixing pushing unit 2, an electric control unit 3, a constant-temperature storage stirring unit 4, an intermittent rotating unit 5, an automatic continuous pouring unit 6 and a disinfection and sterilization unit 7.
In fig. 3: 101 front housing, 104 right housing, 105 upper housing, 302 control panel, 304 display screen.
In fig. 4: 401 liquid-filled tanks, 402 heat-preserving barrels, 403 electric heating plates, 404 partition boards, 405 temperature sensors and 406 electric stirrers.
In fig. 5: 201 lifting locating ring, 202 spring, 203 rotating disc, 204 pushing square disc, 502 driving shaft, 503 driving wheel, 504 rotating arm, 505 rotating rod, 506 grooved wheel and 507 output shaft.
In fig. 6: 102 a rear shell, 103 a left shell, 106 a lower shell, 201 a lifting positioning ring, 203 a rotating disc, 204 a pushing square disc, 205 a pushing guide rail, 206 a positioning magnet, 301 a control circuit, 303 a control box, 402 a heat-preserving barrel, 406 an electric stirrer, 501 a three-phase asynchronous motor, 502 a driving shaft, 507 an output shaft, 601 a pouring motor, 602 a peristaltic pump, 603 a liquid inlet pipeline, 604 a liquid outlet pipeline and 701 ultraviolet lamps.
Detailed Description
In order to make the advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
As shown in fig. 2 and 6, a portable continuous automatic preparation device for agar culture medium containing temperature-sensitive active substance comprises: the shell keeps warm supporting element 1, the fixed propelling movement unit 2 of many specifications culture dish, automatically controlled unit 3, constant temperature storage stirring unit 4, intermittent type rotary unit 5, automatic continuous pouring unit 6, disinfection and sterilization unit 7 seven units, its characterized in that: the shell heat-insulating support unit 1 is made of heat-insulating light materials, a push guide rail 205 of the multi-specification culture dish fixing push unit 2 is connected with a left shell 103 and a right shell 104, the electric control unit 3 is connected with the constant-temperature storage stirring unit 4, the intermittent rotating unit 5, the automatic continuous pouring unit 6 and the disinfection and sterilization unit 7 through a control circuit 301, and provides current, the constant-temperature storage stirring unit 4 is connected with the automatic continuous pouring unit 6 through a liquid inlet pipeline 603 and conveys culture liquid, the intermittent rotating unit 5 synchronously rotates a rotating disc 203 of the multi-specification culture dish fixing push unit 2 through an output shaft 507, the intermittent rotating unit 5 and the automatic continuous pouring unit 6 are installed on a lower shell 106 of the shell heat-insulating support unit 1 through a bracket, and the disinfection and sterilization unit 7 is installed on the lower part of an upper shell 105 of the shell heat-insulating support unit 1 through the bracket.
As shown in fig. 2, 3 and 6, the heat-insulating and supporting unit 1 for a shell comprises a front shell 101, a rear shell 102, a left shell 103, a right shell 104, an upper shell 105 and a lower shell 106, wherein the front shell 101, the rear shell 102, the left shell 103, the right shell 104, the upper shell 105 and the lower shell 106 are made of heat-insulating and light materials, the whole box body is small in structure and light in weight, the portability is good, the front shell 101 is provided with a pushing port 107, the pushing port 107 can allow pushing of the multi-specification culture dish fixing pushing unit 2, the rear shell 102 is connected with other shells through bolts, and the rear shell 102 is convenient to open so as to detach the liquid containing tank 401, the liquid inlet pipeline 603 and the liquid outlet pipeline 604.
As shown in fig. 2, 5 and 6, the multi-specification culture dish fixing pushing unit 2 comprises a lifting positioning ring 201, a spring 202, a rotating disc 203, a pushing square disc 204, a pushing guide rail 205 and a positioning magnet 206, wherein the pushing guide rail 205 is symmetrically arranged on the left shell 103 and the right shell 104, the positioning magnet 206 is symmetrically arranged on the pushing guide rail 205, a square groove is formed at the symmetrical axis position of the bottom surface of the pushing square disc 204, the width is 5mm longer than the upper end edge of the output shaft 507, the upper end of the output shaft 507 can move to the central position along the square groove, the left side surface and the right side surface are symmetrically provided with the square groove, the pushing square disc 204 can be arranged on the pushing guide rail 205 to slide, the rear surface of the non-grooved surface is covered with an iron sheet, the iron sheet and the positioning magnet 206 are sucked together to play a positioning and fixing role when the pushing square disc 204 is inserted, a round groove is formed on the upper surface, the diameter is larger than 2mm of the diameter of the rotating disc 203, a round hole is formed in the center, the diameter is larger than the maximum diameter of the output shaft 507 by 5mm, square grooves with the width being larger than the length of the upper end edge of the output shaft 507 by 5mm are formed in the bottom surface of the rotating disc 203, square grooves with the size being larger than the length of the original square groove by 5mm are formed in the center, the size is matched with the upper end of the output shaft 507, the output shaft 507 can be allowed to be pushed into the square grooves in the center along the square grooves on the bottom surface of the pushing square disc 204 and the square grooves with the diameter of the bottom surface of the rotating disc 203, so that motion is transmitted, four round grooves are formed in the round grooves, upright columns are regularly distributed in the round grooves, three springs 202 are arranged in each round, one end of each spring 202 is sleeved on the upright column in the round grooves, the other end of each spring 202 is sleeved on a protruding block of the lifting positioning ring 201, concentric gaps of the lifting positioning rings 201 with different sizes are installed in the round grooves, the outer diameter of the lifting positioning ring 201 is set to be larger than the size of a common standard flat dish by 2mm, when plates of different specifications are mounted on the lifting positioning ring 201, the spring 202 and the lifting positioning ring 201 with the inner diameter smaller than the diameter of the plate are pressed down under the action of gravity, so that the positioning and fixing functions are realized.
As shown in fig. 2 and 6, the electronic control unit 3 includes a control circuit 301, a control panel 302, a control box 303, and a display screen 304, where the display screen 304 and the control panel 302 are mounted on the front housing 101, and the control panel 302 performs signal transmission with the display screen 304, the electric heating sheet 403, the temperature sensor 405, the electric stirrer 406, the three-phase asynchronous motor 501, the pouring motor 601, and the ultraviolet lamp 701 through the control circuit 301, the control box 303, and thus, the start and stop of each component can be controlled by operating keys on the control panel 302.
As shown in fig. 2, fig. 4, and fig. 6, the constant temperature storage stirring unit 4 includes a liquid containing tank 401, a heat preserving barrel 402, an electric heating plate 403, a partition 404, a temperature sensor 405, and an electric stirrer 406, where the heat preserving barrel 402 is made of heat preserving and insulating light material, the partition 404 is provided with the electric heating plate 403 and the temperature sensor 405 in the lower part of the partition 404, a through hole is provided on the partition 404, water can flow, the liquid containing tank 401 is installed on the upper part, the liquid containing tank 401 is detachable and made of high temperature and high pressure resistant glass material, the liquid containing tank is installed on the partition 404, a vent hole, a liquid outlet and a stirring opening are provided on the tank cover, a bacterial filtering film is contained in the vent hole, a liquid inlet pipeline 603 is installed on the liquid outlet, the electric stirrer 406 is installed on the stirring opening, and the electric stirrer 406 is started to work after the equipment is installed, so as to mix the heat-sensitive substances and the agar culture medium solution.
As shown in fig. 2, 5 and 6, the intermittent rotation unit 5 includes a three-phase asynchronous motor 501, a driving shaft 502, a driving wheel 503, a rotating arm 504, a rotating rod 505, a sheave 506 and an output shaft 507, wherein the three-phase asynchronous motor 501 is connected with the driving shaft 502 through a coupling, the driving shaft 502 is connected with the driving wheel 503 through a key, the driving wheel 503 is in interference fit with the rotating arm 504, the rotating rod 505 is connected in a shaft hole of the rotating arm 504 through a key, the rotating arm 504 is in matched movement with the sheave 506, the output shaft 507 is connected with the sheave 506 through a key, the upper end of the output shaft 507 is square, the side length is slightly smaller than the width of a square groove at the diameter position of the bottom of the rotating disc 203 and a square groove at the symmetrical axis position of the pushing square disc 204, and the rotating disc 203 is driven to intermittently rotate periodically under the control of the electronic control unit 3.
As shown in fig. 2 and 6, the automatic continuous pouring unit 6 includes a pouring motor 601, a peristaltic pump 602, a liquid inlet pipe 603 and a liquid outlet pipe 604, the pouring motor 601 controls the peristaltic pump 602 to absorb liquid and discharge liquid, the liquid inlet pipe 603 and the liquid outlet pipe 604 are respectively installed at the liquid inlet and the liquid outlet of the peristaltic pump 602, the liquid inlet pipe 603 and the liquid outlet pipe 604 are detachable and made of high-temperature and high-pressure resistant glass materials, and can be easily replaced, cleaned and sterilized as the liquid containing tank 401, thereby realizing the preparation of various culture mediums.
As shown in fig. 6, the sterilization unit 7 includes an ultraviolet lamp 701, and the ultraviolet lamp 701 is in a closed state after the start of pouring, thereby preventing deactivation of the temperature-sensitive active substance.
As shown in fig. 3 and 6, a continuous automatic preparation method of a portable agar culture medium containing temperature-sensitive active substances is characterized by comprising the following steps:
s1: opening the rear shell 102, and installing the peristaltic pump 602, the liquid inlet pipeline 603 and the liquid outlet pipeline 604 which are subjected to cleaning, disinfection, sterilization and drying treatment into the equipment in a sterile environment;
s2: pressing a temperature control key, starting a device temperature control system to operate, and after the temperature displayed on the display screen 304 reaches the set temperature, automatically adjusting the temperature of the electric heating sheet 403 by the temperature control system through the temperature sensor 405 so that the system keeps constant temperature all the time;
s3: the method comprises the steps of installing a liquid containing tank 401 filled with agar solution after pressure steam sterilization, adding a temperature-sensitive substance from a liquid outlet when the temperature is cooled to be constant to a required constant temperature, connecting a liquid inlet pipeline 603, pressing a stirring sterilization key on a control panel 302, starting to automatically stir the solution in the liquid containing tank 401 while an ultraviolet lamp 701 starts to work, and stopping stirring automatically when the set stirring and sterilization time is reached, wherein the ultraviolet lamp 701 is also closed simultaneously;
s4: placing the sterilized and dried plate on the rotating disc 203 to fix in four circular grooves formed on the rotating disc 203, matching the rotating disc 203 with the pushing square disc 204, namely aligning the axes of square grooves formed on the lower surface, and inserting square grooves formed on the left and right sides of the pushing square disc 204 into working positions along the pushing guide rail to contact with the positioning magnet 206;
s5: and (3) automatically and continuously pouring the agar culture medium solution containing the temperature-sensitive active substances by pressing a pouring button, and repeatedly pouring after the automatic stopping of the operation is finished when the set pouring time is reached, withdrawing the pushing square plate 204 and replacing the poured culture plate.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. A portable continuous automatic preparation device for agar culture medium containing temperature-sensitive active substances comprises: the shell keeps warm supporting element (1), fixed propelling movement unit (2) of many specifications culture dish, automatically controlled unit (3), constant temperature storage stirring unit (4), intermittent type rotary unit (5), automatic continuous pouring unit (6), disinfection and sterilization unit (7) seven units, its characterized in that: the shell heat-insulating support unit (1) is made of heat-insulating light materials, a push guide rail (205) of the multi-specification culture dish fixing push unit (2) is connected with a left shell (103) and a right shell (104), the electric control unit (3) is connected with the constant-temperature storage stirring unit (4), the intermittent rotation unit (5), the automatic continuous pouring unit (6) and the disinfection and sterilization unit (7) through a control circuit (301), and provides current, the constant-temperature storage stirring unit (4) is connected with the automatic continuous pouring unit (6) through a liquid inlet pipeline (603) and conveys culture liquid, the intermittent rotation unit (5) enables a rotating disc (203) of the multi-specification culture dish fixing push unit (2) to synchronously rotate through an output shaft (507), the intermittent rotation unit (5) and the automatic continuous pouring unit (6) are arranged on a lower shell (106) of the shell heat-insulating support unit (1) through a bracket, and the disinfection and sterilization unit (7) is arranged on the lower part of an upper shell (105) of the shell heat-insulating support unit (1) through the bracket;
the multi-specification culture dish fixing pushing unit (2) comprises a lifting positioning ring (201), springs (202), a rotating disc (203), a pushing square disc (204), a pushing guide rail (205) and positioning magnets (206), wherein the pushing guide rail (205) is symmetrically arranged on the left shell (103) and the right shell (104), the positioning magnets (206) are symmetrically arranged on the pushing guide rail (205) left and right, square grooves are formed in the symmetrical axis position of the bottom surface of the pushing square disc (204), square grooves are symmetrically formed in the left and right sides, iron sheets are covered on the ungrooved rear surface, round grooves are formed in the upper surface, a round hole is formed in the center, square grooves which are deeper than the original square grooves by 5mm are formed in the bottom surface of the rotating disc (203), four round grooves are regularly distributed in the round grooves, three springs (202) are arranged in each circle, one end of each spring (202) is sleeved on each round groove, the other end of each round groove is sleeved on a protruding block of the lifting positioning ring (201), the lifting positioning ring (201) with different sizes is concentric, and the lifting positioning ring (201) is arranged in the round groove with the outer diameter of the large-specification dish 2;
the intermittent rotation unit (5) comprises a three-phase asynchronous motor (501), a driving shaft (502), a driving wheel (503), a rotating arm (504), a rotating rod (505), a grooved wheel (506) and an output shaft (507), wherein the three-phase asynchronous motor (501) is connected with the driving shaft (502) through a coupler, the driving shaft (502) is connected with the driving wheel (503) through a key, the driving wheel (503) is in interference fit with the rotating arm (504), the rotating rod (505) is connected in a shaft hole of the rotating arm (504) through a key, the rotating arm (504) moves in cooperation with the grooved wheel (506), the output shaft (507) is connected with the grooved wheel (506) through a key, the upper end of the output shaft (507) is square, and the rotating disc (203) is driven to intermittently rotate in a period under the control of the electronic control unit (3);
the automatic continuous pouring unit (6) comprises a pouring motor (601), a peristaltic pump (602), a liquid inlet pipeline (603) and a liquid outlet pipeline (604), wherein the pouring motor (601) controls the peristaltic pump (602) to absorb liquid and discharge liquid, the liquid inlet pipeline (603) and the liquid outlet pipeline (604) are respectively arranged at the liquid inlet and the liquid outlet of the peristaltic pump (602), and the liquid inlet pipeline (603) and the liquid outlet pipeline (604) are detachable and made of high-temperature and high-pressure resistant glass materials.
2. The portable continuous automatic preparation device for agar culture medium containing temperature-sensitive active substances according to claim 1, wherein the continuous automatic preparation device is characterized in that: the shell heat preservation supporting unit (1) comprises a front shell (101), a rear shell (102), a left shell (103), a right shell (104), an upper shell (105) and a lower shell (106), wherein the front shell (101), the rear shell (102), the left shell (103), the right shell (104), the upper shell (105) and the lower shell (106) are made of heat preservation and heat insulation light materials, the front shell (101) is provided with a pushing port (107), the pushing port (107) can allow pushing of the multi-specification culture dish fixing pushing unit (2), and the rear shell (102) is connected with other shells through bolts.
3. The portable continuous automatic preparation device for agar culture medium containing temperature-sensitive active substances according to claim 1, wherein the continuous automatic preparation device is characterized in that: the electric control unit (3) comprises a control circuit (301), a control panel (302), a control box (303) and a display screen (304), wherein the display screen (304) and the control panel (302) are arranged on the front shell (101), and the control panel (302) carries out signal transmission between the control circuit (301), the control box (303) and the display screen (304), an electric heating plate (403), a temperature sensor (405), an electric stirrer (406), a three-phase asynchronous motor (501), a pouring motor (601) and an ultraviolet lamp (701).
4. The portable continuous automatic preparation device for agar culture medium containing temperature-sensitive active substances according to claim 1, wherein the continuous automatic preparation device is characterized in that: constant temperature storage stirring unit (4) are including dress fluid reservoir (401), heat preservation bucket (402), electric heat piece (403), baffle (404), temperature sensor (405), electric stirrer (406), adopt water bath heating, heat preservation bucket (402) are thermal-insulated light material of heat preservation, establish baffle (404) in, baffle (404) lower part is equipped with electric heat piece (403) and temperature sensor (405), have the through-hole on baffle (404), install fluid reservoir (401) on upper portion, dress fluid reservoir (401) can dismantle and be high temperature resistant high pressure glass material, install on the baffle, the cover is equipped with air vent, liquid outlet, stirring mouth, the strain membrane is contained to the air vent, feed liquor pipeline (603) is installed to the liquid outlet, electric stirrer (406) is installed to the stirring mouth.
5. The portable continuous automatic preparation device for agar culture medium containing temperature-sensitive active substances according to claim 1, wherein the continuous automatic preparation device is characterized in that: the disinfection and sterilization unit (7) comprises an ultraviolet lamp (701), wherein the ultraviolet lamp (701) is in a closed state after the start of pouring.
6. A continuous automatic preparation method of a portable agar culture medium containing temperature-sensitive active substances is characterized by comprising the following steps: the method is suitable for the portable continuous automatic preparation device of the agar culture medium containing the temperature-sensitive active substances, which is characterized by comprising the following steps:
s1: opening the rear shell (102), and installing a peristaltic pump (602), a liquid inlet pipeline (603) and a liquid outlet pipeline (604) which are subjected to cleaning, disinfection, sterilization and drying treatment into the equipment in a sterile environment;
s2: pressing a temperature control key, starting a temperature control system of the equipment to work, and after the temperature displayed on a display screen (304) reaches a set temperature, automatically adjusting the temperature of an electric heating sheet (403) by the temperature control system through a temperature sensor (405) so that the system is kept constant temperature all the time;
s3: installing a liquid containing tank (401) filled with agar solution after pressure steam sterilization, adding a temperature-sensitive substance from a liquid outlet when the temperature is cooled to be constant to a required constant temperature, then connecting a liquid inlet pipeline (603), pressing a stirring sterilization key on a control panel (302), starting to automatically stir the solution in the liquid containing tank (401) and simultaneously starting an ultraviolet lamp (701), stopping stirring automatically when the set stirring and sterilization time is reached, and closing the ultraviolet lamp (701) simultaneously;
s4: placing the sterilized and dried plate on a rotating disc (203) to be fixed in four circular grooves, matching the rotating disc (203) with a pushing square disc (204) to align the axes of the square grooves formed on the lower surface, and inserting the square grooves formed on the left and right sides of the pushing square disc (204) into working positions along a pushing guide rail to be in contact with a positioning magnet (206);
s5: and (3) pressing a pouring button to automatically and continuously pour the agar culture medium solution containing the temperature-sensitive active substances, and after the set pouring time is reached, automatically stopping the operation, namely finishing pouring, withdrawing the pushing square plate (204), and replacing the poured culture dish to repeatedly pour.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910162220.7A CN109810893B (en) | 2019-03-05 | 2019-03-05 | Portable continuous automatic preparation device and method for agar culture medium containing temperature-sensitive active substances |
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| CN201910162220.7A CN109810893B (en) | 2019-03-05 | 2019-03-05 | Portable continuous automatic preparation device and method for agar culture medium containing temperature-sensitive active substances |
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| CN109810893B true CN109810893B (en) | 2024-03-08 |
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| GB835354A (en) * | 1957-04-01 | 1960-05-18 | Getinge Mek Verkst S Aktiebola | Improvements in or relating to incubation plants |
| CN103874759A (en) * | 2011-04-29 | 2014-06-18 | Bd克斯特拉有限公司 | Disc dispensing device, tubular container for use in such a disc dispensing device and method of dispensing discs |
| CN204474657U (en) * | 2015-03-03 | 2015-07-15 | 浙江大学 | Portable agar plate pours into device |
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