CN109810893A - A kind of portable agar medium of substance containing temperature sensitive active continuously automatic preparation facilities and method - Google Patents
A kind of portable agar medium of substance containing temperature sensitive active continuously automatic preparation facilities and method Download PDFInfo
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- CN109810893A CN109810893A CN201910162220.7A CN201910162220A CN109810893A CN 109810893 A CN109810893 A CN 109810893A CN 201910162220 A CN201910162220 A CN 201910162220A CN 109810893 A CN109810893 A CN 109810893A
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- 229920001817 Agar Polymers 0.000 title claims abstract description 39
- 239000008272 agar Substances 0.000 title claims abstract description 39
- 239000000126 substance Substances 0.000 title claims abstract description 33
- 238000002360 preparation method Methods 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title abstract description 10
- 230000001954 sterilising effect Effects 0.000 claims abstract description 30
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 23
- 239000007788 liquid Substances 0.000 claims description 26
- 238000009413 insulation Methods 0.000 claims description 17
- 239000000463 material Substances 0.000 claims description 17
- 238000003756 stirring Methods 0.000 claims description 17
- 238000005192 partition Methods 0.000 claims description 15
- 230000002572 peristaltic effect Effects 0.000 claims description 13
- 108090000565 Capsid Proteins Proteins 0.000 claims description 12
- 238000005485 electric heating Methods 0.000 claims description 12
- 238000004321 preservation Methods 0.000 claims description 9
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 8
- 239000011521 glass Substances 0.000 claims description 8
- 238000003860 storage Methods 0.000 claims description 8
- 244000309464 bull Species 0.000 claims description 7
- 238000004140 cleaning Methods 0.000 claims description 5
- 229910052742 iron Inorganic materials 0.000 claims description 4
- 238000003825 pressing Methods 0.000 claims description 4
- 230000000721 bacterilogical effect Effects 0.000 claims description 3
- 238000005266 casting Methods 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 230000008878 coupling Effects 0.000 claims description 3
- 238000010168 coupling process Methods 0.000 claims description 3
- 238000005859 coupling reaction Methods 0.000 claims description 3
- 238000009826 distribution Methods 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 230000005611 electricity Effects 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 239000008236 heating water Substances 0.000 claims description 3
- 238000005213 imbibition Methods 0.000 claims description 3
- 238000012549 training Methods 0.000 claims description 2
- 230000002146 bilateral effect Effects 0.000 claims 1
- 235000015097 nutrients Nutrition 0.000 claims 1
- 238000000926 separation method Methods 0.000 claims 1
- 238000002474 experimental method Methods 0.000 abstract description 7
- 239000011149 active material Substances 0.000 abstract description 2
- 239000003814 drug Substances 0.000 abstract description 2
- 239000002609 medium Substances 0.000 description 13
- 238000010586 diagram Methods 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000011177 media preparation Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000007711 solidification Methods 0.000 description 2
- 230000008023 solidification Effects 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 239000013543 active substance Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 239000003562 lightweight material Substances 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention discloses the portable agar medium of substance containing temperature sensitive active of one kind continuously automatic preparation facilities and method, support unit is kept the temperature including shell, the fixed push unit of more specification culture dishes, electronic control unit, constant temperature stores agitating unit, intermittent rotary unit, automatically continuously pour into unit, sterilization unit, the present invention designs for detachable portable, it designs small and exquisite, it can be placed and operated under the limited environment of the spaces such as superclean bench completely, seven unit co-ordinations, it can effectively ensure that the constant-temperature quantitative of active material pours into, to reach medicine, the requirement of biological experiment homogeneity, fill up the blank of the continuous pourers of more specification substance containing the temperature sensitive active agar mediums of domestic use for laboratory.
Description
Technical field
The present invention relates to a kind of biology or medical experiment apparatus field, specially a kind of portable substance containing temperature sensitive active
Agar medium continuously automatic preparation facilities and method.
Background technique
The preparation of medicine and the common Agar Plating of biological experiment need to through weighing, pressuresteam sterilization, pour into
Packing and etc..Temperature is excessively high to be easily destroyed temperature sensitive species activity, and temperature is lower to will lead to agar solidification, and most of agar exists
40 DEG C or so start to solidify, and the agar of solidification needs to be heated to 95 DEG C and just starts to melt, therefore, certain special agar mediums
And blood, serum, antibiotic, indicator bacteria etc. need to be often added to thermo-responsive substance 45 DEG C or so of constant temperature fine jade in antibacterial experiment etc.
The agar medium containing temperature sensitive substance is prepared in rouge, needs strict temperature control during the preparation process.In addition, equal for experiment
One property requires to must assure that quantifying for active material, needs quantitatively to pour into agar and prepares agar plate.Therefore, object containing temperature sensitive active
Matter agar medium needs to guarantee the temperature uniformity of operating process, aseptic and the conditions such as quantitative.
Presently commercially available agar plate majority is large scale equipment mass production without temperature sensitive substance or containing only Common Species
Class and contain quantity of material, and because temperature sensitive substance shelf-life shorter transportational process is apt to deteriorate, preferably ready-to-use, therefore, it is difficult to meet
Laboratory is because needing to add needed for the individual character experiment of variety classes and the temperature sensitive substance of content.At present prior art there are the problem of master
It wants are as follows:
(1) production of batch production large size culture medium Preparation equipment is of large quantities, but the kinds of culture medium produced is few, is unable to satisfy reality
Room is tested because type and content of the experiment sample diversity to temperature sensitive substance require changeable requirement.
(2) small-sized Preparation equipment can not be accomplished continuously to pour into automatically, in addition without and meanwhile fixed multiple plates and size can
The fixed device of the plate of adjusting, working efficiency are low.
It can be seen that, it would be highly desirable to develop a kind of portable agar medium of substance containing temperature sensitive active of suitable laboratory research
Continuous automatic preparation facilities and method.
Summary of the invention
In view of the above-mentioned problems, the present invention proposes that a kind of manufacturing cost is small, multiple functional, operation is simple and reliable, and homogeneity is good
The portable agar medium of substance containing temperature sensitive active continuously automatic preparation facilities and method.
To achieve the above object, the invention provides the following technical scheme: a kind of portable agar of substance containing temperature sensitive active training
The continuous automatic preparation facilities of feeding base, comprising: shell keep the temperature the fixed push unit of support unit, more specification culture dishes, electronic control unit,
Constant temperature storage agitating unit, intermittent rotary unit automatically continuously pour into seven unit, sterilization unit units, and feature exists
In: the shell heat preservation support unit uses insulation light material, and the fixed push unit of more specification culture dishes pushes away
Send guide rail to be linked together with left shell, right shell body, the electronic control unit by control circuit and constant temperature store agitating unit,
Have a rest rotary unit, automatically continuously pour into unit, sterilization element connection, and provide electric current, the constant temperature stores agitating unit
It by input duct and automatically continuously pours into element connection and conveys culture solution, the intermittent rotary unit is made more by output shaft
The rotating circular disc of the fixed push unit of specification culture dish rotates synchronously, and the intermittent rotary unit, the automatic continuous unit that pours into lead to
It crosses bracket to be mounted in the lower case of shell heat preservation support unit, the sterilization unit is mounted on shell by bracket and keeps the temperature
The upper housing lower part of support unit.
Preferably, shell heat preservation support unit include procapsid, back casing, left shell, right shell body, upper housing, under
Shell, the procapsid, back casing, left shell, right shell body, upper housing, lower case are insulation light material, procapsid
Equipped with push mouth, push mouth allows the push of the fixed push unit of more specification culture dishes, and back casing and other shells are bolt
Connection.
Preferably, the fixed push unit of more specification culture dishes includes lifting positioning ring, spring, rotating circular disc, push
Square plate, push guide rail, phase magnet, the push guide rail are left and right symmetrically arranged on left shell, right shell body, are pushed left on guide rail
The right side is symmetrically installed phase magnet, and push square plate bottom surface axis of symmetry position is provided with square groove, and left and right side is symmetrically provided with square groove, does not open
It is covered with iron plate behind slot, is provided with circular groove above, center is provided with circular hole, and rotating circular disc basal diameter position is provided with square groove, in
Center portion is separated by the square groove for being deeper than former square groove 5mm, is opened above there are four circular groove, three columns of rule distribution in circular groove, often
There are three spring, spring one ends to cover on circular groove inner column for circle, and another end cap is on the casting lug thereon of lifting positioning ring, different size
Lifting positioning ring concentric gap is mounted in circular groove, and lifting positioning ring outer diameter is set greater than regular size plate size 2mm.
Preferably, the electronic control unit includes control circuit, control panel, control cabinet, display screen, the display screen and control
Panel processed is mounted on procapsid, and control panel passes through control circuit, control cabinet and display screen, electric heating piece, temperature sensor, electricity
Dynamic blender, threephase asynchronous machine pour into progress signal transmitting between motor, ultraviolet radiator.
Preferably, constant temperature storage agitating unit include tank, insulation barrel, electric heating piece, partition, temperature sensor,
Electric mixer, using heating water bath, the insulation barrel is insulation light material, inside sets partition, and the lower partition is equipped with electricity
Backing and temperature sensor have through-hole on partition, and top is equipped with tank, and tank is detachable and is high temperature high voltage resistant glass
Material is mounted on partition, and cover is equipped with venthole, liquid outlet, stirring port, and venthole includes bacteriological filtration film, and liquid outlet is equipped with
Input duct, stirring port are equipped with electric mixer.
Preferably, the intermittent rotary unit include threephase asynchronous machine, driving shaft, driving wheel, pivoted arm, bull stick, sheave,
Output shaft, the threephase asynchronous machine are coupled by shaft coupling with driving shaft, and driving shaft is coupled by key with driving wheel, driving wheel
It is interference fit with pivoted arm, for bull stick through key connecting in the axis hole of pivoted arm, pivoted arm and sheave cooperative movement, output shaft pass through key
Couple with sheave, output shaft upper end is rectangular, and drives rotating circular disc period intermittent rotary under electronic control unit control.
Preferably, the unit that automatically continuously pours into includes pouring into motor, peristaltic pump, input duct, liquid outlet pipe, described
Motor control peristaltic pump imbibition drain is poured into, input duct, liquid outlet pipe are separately mounted to the liquid inlet and outlet of peristaltic pump, feed liquor
Pipeline, liquid outlet pipe are detachable and are high temperature high voltage resistant glass material.
Preferably, the sterilization unit includes ultraviolet radiator, and the ultraviolet radiator is after starting to pour into closing
State.
Continuously automatic preparation method, preparation process feature include the portable agar medium of substance containing temperature sensitive active of one kind
Following steps:
S1: opening back casing, and peristaltic pump that cleaning and sterilizing sterilizing-drying is disposed, input duct, liquid outlet pipe are existed
It is installed in equipment under gnotobasis；
S2: pressing temperature control key, opens the work of equipment temperature control system, until displays temperature reaches set temperature on display screen
Afterwards, temperature control system, which automatically adjusts electric heating piece temperature by temperature sensor, makes system remain constant temperature；
S3: the tank equipped with agar solution after pressuresteam sterilization is installed, constant to required to temperature cooling
Thermostat temperature is added temperature sensitive substance from liquid outlet, then input duct is connected, and presses the stirring sterilizing key on control panel,
Starting solution in automatic stirring tank, ultraviolet radiator is started to work simultaneously, and setting stirring to be achieved and sterilization time stop automatically
Work is only stirred, ultraviolet radiator also simultaneously closes off；
S4: the plate that sterilization has been dried is placed in rotating circular disc to open in four circular grooves and fix, and will turn
Dynamic disk and push square plate i.e. following institute's evolution fluted shaft line of working good are aligned, then will push square plate left and right side institute evolution slot edge
Push guide rail is inserted into operating position and contacts with phase magnet；
S5: it presses and pours into key and automatically continuously carry out pouring into for the agar medium solution of substance containing temperature sensitive active.It is to be achieved
The time is poured into setting, is automatically stopped work and pours into and finishes, and extraction pushes square plate, and the culture dish poured under replacement can weigh
It pours into again.
Compared with prior art, the beneficial effects of the present invention are:
(1) portable small and exquisite, it is easy to operate.While meeting special culture medium preparation condition, the present invention is small and exquisite portable,
Flexibility is good, agar culture needed for the individual character that suitable laboratory prepares the substance containing temperature sensitive active of variety classes and content is tested
Base.
(2) it continuously pours into automatically.The fixed device of plate for possessing while fixing multiple plates and plate dimension adjustable, can
Multiple plates are continuously poured into automatically, can theoretically prepare the agar medium of any type and specification.
(3) equipment disassembly, cleaning sterilizing is convenient.What tank, input duct, liquid outlet pipe etc. were contacted with culture medium solution
Components are designed using detachable, and material is high temperature high voltage resistant glass, and disassembly, cleaning sterilizing is convenient.
Detailed description of the invention
Fig. 1 is appearance diagram of the present invention
Fig. 2 is each unit schematic diagram of the present invention
Fig. 3 is shell schematic diagram of the present invention
Fig. 4 is that constant temperature of the present invention stores agitating unit part schematic diagram
Fig. 5 is the fixed push unit part schematic diagram of the more specification culture dishes of the present invention
Fig. 6 is parts schematic diagram of the present invention
In Fig. 2: 1 shell keeps the temperature the fixed push unit of support unit, more than 2 specification culture dishes, 3 electronic control unit, the storage of 4 constant temperature
Unit, 7 sterilization units are automatically continuously poured into agitating unit, 5 intermittent rotary units, 6.
In Fig. 3: 101 procapsids, 104 right shell bodies, 105 upper housings, 302 control panels, 304 display screens.
In Fig. 4: 401 tanks, 402 insulation barrels, 403 electric heating pieces, 404 partitions, 405 temperature sensors, 406 electric stirrings
Device.
In Fig. 5: 201 lifting positioning rings, 202 springs, 203 rotating circular discs, 204 push square plates, 502 driving shafts, 503 are actively
Wheel, 504 pivoted arms, 505 bull sticks, 506 sheaves, 507 output shafts.
In Fig. 6: 102 back casings, 103 left shells, 106 lower cases, 201 lifting positioning rings, 203 rotating circular discs, 204 push
Square plate, 205 push guide rails, 206 phase magnets, 301 control circuits, 303 control cabinets, 402 insulation barrels, 406 electric mixers,
The liquid out of motor, 602 peristaltic pumps, 603 input ducts, 604 is poured into 501 threephase asynchronous machines, 502 driving shafts, 507 output shafts, 601
Pipeline, 701 ultraviolet radiator.
Specific embodiment
The advantages of to make the embodiment of the present invention, is clearer, and following will be combined with the drawings in the embodiments of the present invention, to this hair
Technical solution in bright embodiment is clearly and completely described, it is clear that described embodiment is only present invention a part
Embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not making wound
Every other embodiment obtained under the premise of the property made labour, shall fall within the protection scope of the present invention.
As shown in Fig. 2, Fig. 6, a kind of continuous automatic preparation facilities of the portable agar medium of substance containing temperature sensitive active is wrapped
Include: shell keep the temperature the fixed push unit 2 of support unit 1, more specification culture dishes, electronic control unit 3, constant temperature storage agitating unit 4,
Have a rest rotary unit 5, automatically continuously pour into 7 seven unit 6, sterilization unit units, it is characterised in that: the shell heat preservation branch
It supports unit 1 and uses insulation light material, the push guide rail 205 and left housing of the fixed push unit 2 of more specification culture dishes
Body 103, right shell body 104 are linked together, the electronic control unit 3 by control circuit 301 and constant temperature store agitating unit 4,
Have a rest rotary unit 5, automatically continuously pour into unit 6, sterilization unit 7 couples, and provides electric current, the constant temperature storage stirring is single
Member 4 couples and conveys culture solution with unit 6 is automatically continuously poured by input duct 603, and the intermittent rotary unit 5 passes through defeated
Shaft 507 rotates synchronously the rotating circular disc 203 of the fixed push units 2 of more specification culture dishes, the intermittent rotary unit 5, from
The dynamic unit 6 that continuously pours into is mounted in the lower case 106 of shell heat preservation support unit 1 by bracket, the sterilization unit 7
105 lower part of upper housing that shell keeps the temperature support unit 1 is mounted on by bracket.
As shown in Fig. 2, Fig. 3, Fig. 6, the shell heat preservation support unit 1 includes procapsid 101, back casing 102, left shell
103, right shell body 104, upper housing 105, lower case 106, the procapsid 101, back casing 102, left shell 103, right shell body
104, upper housing 105, lower case 106 are insulation light material, and the entire small light weight of body structure, portability is preferable,
Procapsid 101 is equipped with push mouth 107, and push mouth 107 allows the push of the fixed push unit 2 of more specification culture dishes, back casing
102 with other shells be bolt-connection, is opened convenient for back casing 102 to dismantling tank 401, input duct 603, outlet tube
Road 604.
As shown in figure 2, figure 5 and figure 6, the fixed push unit 2 of more specification culture dishes includes lifting positioning ring 201, spring
202, rotating circular disc 203, push square plate 204, push guide rail 205, phase magnet 206, the push guide rail 205 are symmetrically pacified
It on left shell 103, right shell body 104, pushes and is left and right symmetrically arranged phase magnet 206 on guide rail 205, push 204 bottom of square plate
The symmetrical axial location in face is provided with square groove, and width is greater than 507 upper end side length 5mm of output shaft, and 507 upper end edge square groove of output shaft can be made to move
It moves to center, left and right side is symmetrically provided with square groove, push square plate 204 can be made to be mounted on push guide rail 205 and slide, do not open
Iron plate is covered with behind slot, iron plate and phase magnet 206 are inhaled together to play positioning and solid when pushing square plate 204 and being inserted into
It is set for using, is provided with circular groove above, diameter is greater than 203 diameter 2mm of rotating circular disc, and center is provided with circular hole, and diameter is greater than output shaft 507
Maximum gauge 5mm, 203 basal diameter position of rotating circular disc are provided with square groove, and width is greater than 507 upper end side length 5mm of output shaft, center
Part is provided with the square groove for being deeper than former square groove 5mm, and size is matched with 507 upper end of output shaft, and permissible output shaft 507 is along pushing away
204 bottom surface square groove of square plate and 203 basal diameter position square groove of rotating circular disc is sent to be pushed in central part square groove, to pass
Movement is passed, is opened above there are four circular groove, rule distribution column in circular groove, there are three spring 202,202 1 end caps of spring to exist for every circle
On circular groove inner column, for another end cap on the casting lug thereon of lifting positioning ring 201, different size goes up and down 201 concentric gap of positioning ring
It is mounted in circular groove, lifting 201 outer diameter of positioning ring is set greater than regular size plate size 2mm, when different size plate is pacified
The meeting lifting positioning ring smaller than plate diameter because of gravity dead spring 202 and internal diameter when on lifting positioning ring 201
201, to play positioning and fixed function.
As shown in Fig. 2, Fig. 6, the electronic control unit 3 includes control circuit 301, control panel 302, control cabinet 303, display
Screen 304, the display screen 304 are mounted on procapsid 101 with control panel 302, control panel 302 by control circuit 301,
Control cabinet 303 and display screen 304, temperature sensor 405, electric mixer 406, threephase asynchronous machine 501, incline at electric heating piece 403
It infuses and carries out signal transmitting between motor 601, ultraviolet radiator 701, so as to by operating on panel 302 processed by each portion of key control
The start and stop of part.
As described in Fig. 2, Fig. 4, Fig. 6 constant temperature storage agitating unit 4 include tank 401, insulation barrel 402, electric heating piece 403,
Partition 404, temperature sensor 405, electric mixer 406, using heating water bath, the insulation barrel 402 is insulation lightweight
Material, inside sets partition 404, and 404 lower part of partition is equipped with electric heating piece 403 and temperature sensor 405, has through-hole on partition 404, can permit
Perhaps water flowing, top are equipped with tank 401, and tank 401 is detachable and is high temperature high voltage resistant glass material, are mounted on partition
On 404, cover is equipped with venthole, liquid outlet, stirring port, and venthole includes bacteriological filtration film, and liquid outlet is equipped with input duct 603,
Stirring port is equipped with electric mixer 406, and electric mixer 406 is activated work after equipment installs, for abundant
Heat-sensitive materials and agar medium solution are mixed.
As shown in figure 2, figure 5 and figure 6, the intermittent rotary unit 5 includes threephase asynchronous machine 501, driving shaft 502, active
503, pivoted arm 504, bull stick 505, sheave 506, output shaft 507 are taken turns, the threephase asynchronous machine 501 passes through shaft coupling and driving shaft
502 connections, driving shaft 502 are coupled by key with driving wheel 503, and driving wheel 503 and pivoted arm 504 are interference fit, and bull stick 505 is logical
Key connecting is crossed in the axis hole of pivoted arm 504, pivoted arm 504 and 506 cooperative movement of sheave, output shaft 507 pass through key and sheave 506
Connect, 507 upper end of output shaft be it is rectangular, it is symmetrical that side length is slightly less than 203 base diameter position square groove of rotating circular disc, push square plate 204
The width of axial location square groove, and 203 period of rotating circular disc intermittent rotary is driven under the control of electronic control unit 3.
As shown in Fig. 2, Fig. 6, the unit 6 that automatically continuously pours into includes pouring into motor 601, peristaltic pump 602, input duct
603, liquid outlet pipe 604, the motor 601 that pours into control 602 imbibition drain of peristaltic pump, input duct 603, liquid outlet pipe 604
It is separately mounted to the liquid inlet and outlet of peristaltic pump 602, input duct 603, liquid outlet pipe 604 are detachable and are high temperature high voltage resistant glass
Glass material can easily be replaced and cleaned sterilizing very much as tank 401, to realize the preparation of various culture mediums.
As shown in fig. 6, the sterilization unit 7 includes ultraviolet radiator 701, the ultraviolet radiator 701 is starting to pour into
After be in close state, prevent from making temperature sensitive active substance to inactivate.
As shown in Fig. 3,6, prepared by a kind of continuous automatic preparation method of the portable agar medium of substance containing temperature sensitive active
Process feature the following steps are included:
S1: opening back casing 102, and the peristaltic pump 602 that cleaning and sterilizing sterilizing-drying is disposed, goes out input duct 603
Liquid pipe road 604 is installed in equipment in an aseptic environment；
S2: pressing temperature control key, opens the work of equipment temperature control system, until displays temperature reaches setting on display screen 304
After temperature, temperature control system, which automatically adjusts 403 temperature of electric heating piece by temperature sensor 405, makes system remain constant temperature；
S3: the tank 401 equipped with agar solution after pressuresteam sterilization is installed, constant to institute to temperature cooling
Thermostat temperature is needed, temperature sensitive substance is added from liquid outlet, then input duct 603 is connected, the stirring pressed on control panel 302 is gone out
Bacterium key, starting solution in automatic stirring tank 401, ultraviolet radiator 701 is started to work simultaneously, and setting to be achieved is stirred and gone out
The bacterium time, it is automatically stopped stirring work, ultraviolet radiator 701 also simultaneously closes off；
S4: the plate that sterilization has been dried is placed in rotating circular disc 203 to open in four circular grooves and fix, will
Rotating circular disc 203 is aligned with push square plate 204 i.e. following institute's evolution fluted shaft line of working good, and then will push 204 left and right sides of square plate
Face institute evolution slot is inserted into operating position along push guide rail and contacts with phase magnet 206；
S5: pressing and pour into key and automatically continuously carry out pouring into for the agar medium solution of substance containing temperature sensitive active, to be achieved
The time is poured into setting, is automatically stopped work and pours into and finishes, and extraction pushes square plate 204, and the culture dish poured under replacement is i.e.
It is repeatable to pour into.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (9)
1. a kind of continuous automatic preparation facilities of portable agar medium of substance containing temperature sensitive active, comprising: shell heat preservation support is singly
First (1), more specification culture dishes fixed push unit (2), electronic control unit (3), constant temperature store agitating unit (4), intermittent rotary list
First (5) automatically continuously pour into (7) seven unit (6), sterilization unit units, it is characterised in that: the shell heat preservation support
Unit (1) uses insulation light material, the push guide rail (205) of the more specification culture dishes fixed push unit (2) with
Left shell (103), right shell body (104) are linked together, and the electronic control unit (3) is stored by control circuit (301) and constant temperature
Agitating unit (4), intermittent rotary unit (5) automatically continuously pour into unit (6), sterilization unit (7) connection, and provide electricity
Stream, constant temperature storage agitating unit (4) are coupled with the automatic continuous unit (6) that pours by input duct (603) and convey training
Nutrient solution, the intermittent rotary unit (5) make the rotation circle of more specification culture dishes fixed push unit (2) by output shaft (507)
Disk (203) rotate synchronously, the intermittent rotary unit (5), automatically continuously pour into unit (6) by bracket be mounted on shell keep the temperature
In the lower case (106) of support unit (1), the sterilization unit (7) is mounted on shell by bracket and keeps the temperature support unit
(1) upper housing (105) lower part.
2. the continuous automatic preparation facilities of the portable agar medium of substance containing temperature sensitive active of one kind according to claim 1,
It is characterized by: shell heat preservation support unit (1) includes procapsid (101), back casing (102), left shell (103), the right side
Shell (104), upper housing (105), lower case (106), the procapsid (101), back casing (102), left shell (103), the right side
Shell (104), upper housing (105), lower case (106) are insulation light material, and procapsid (101) is equipped with push mouth
(107), push mouth (107) allows the push of more specification culture dishes fixed push unit (2), back casing (102) and other shells
Body is bolt-connection.
3. the continuous automatic preparation facilities of the portable agar medium of substance containing temperature sensitive active of one kind according to claim 1,
It is characterized by: more specification culture dishes fixed push unit (2) include lifting positioning ring (201), spring (202), rotation
Disk (203), push square plate (204), push guide rail (205), phase magnet (206), push guide rail (205) bilateral symmetry
It is mounted on left shell (103), on right shell body (104), is left and right symmetrically arranged phase magnet (206) in push guide rail (205), pushed
Square plate (204) bottom surface axis of symmetry position is provided with square groove, and left and right side is symmetrically provided with square groove, is covered with iron behind unslotted
Piece is provided with circular groove above, and center is provided with circular hole, and rotating circular disc (203) basal diameter position is provided with square groove, and central part is provided with depth
It in the square groove of former square groove 5mm, is opened above there are four circular groove, three columns of rule distribution in circular groove, there are three springs for every circle
(202), for (202) one end cap of spring on circular groove inner column, another end cap is different on the casting lug thereon of lifting positioning ring (201)
Size lifting positioning ring (201) concentric gap is mounted in circular groove, and lifting positioning ring (201) outer diameter is set greater than regular size
Plate size 2mm.
4. the continuous automatic preparation facilities of the portable agar medium of substance containing temperature sensitive active of one kind according to claim 1,
It is characterized by: the electronic control unit (3) includes control circuit (301), control panel (302), control cabinet (303), display screen
(304), the display screen (304) and control panel (302) are mounted on procapsid (101), and control panel (302) passes through control
Circuit (301), control cabinet (303) and display screen (304), electric heating piece (403), temperature sensor (405), electric mixer
(406), threephase asynchronous machine (501), pour between motor (601), ultraviolet radiator (701) progress signal transmitting.
5. the continuous automatic preparation facilities of the portable agar medium of substance containing temperature sensitive active of one kind according to claim 1,
It is characterized by: constant temperature storage agitating unit (4) include tank (401), insulation barrel (402), electric heating piece (403), every
Plate (404), temperature sensor (405), electric mixer (406), using heating water bath, the insulation barrel (402) is thermal insulation separation
Hot light material is inside set partition (404), and partition (404) lower part is equipped with electric heating piece (403) and temperature sensor (405), partition
(404) there is through-hole on, top is equipped with tank (401), and tank (401) is detachable and is high temperature high voltage resistant glass material,
It is mounted on partition, cover is equipped with venthole, liquid outlet, stirring port, and venthole includes bacteriological filtration film, and liquid outlet is equipped with inlet tube
Road (603), stirring port are equipped with electric mixer (406).
6. the continuous automatic preparation facilities of the portable agar medium of substance containing temperature sensitive active of one kind according to claim 1,
It is characterized by: the intermittent rotary unit (5) include threephase asynchronous machine (501), driving shaft (502), driving wheel (503),
Pivoted arm (504), bull stick (505), sheave (506), output shaft (507), the threephase asynchronous machine (501) pass through shaft coupling and master
Moving axis (502) connection, driving shaft (502) are coupled by key with driving wheel (503), and driving wheel (503) and pivoted arm (504) are interference
Cooperation, through key connecting in the axis hole of pivoted arm (504), pivoted arm (504) and sheave (506) cooperative movement export bull stick (505)
Axis (507) is coupled by key with sheave (506), and output shaft (507) upper end is rectangular, and is driven under electronic control unit (3) control
Rotating circular disc (203) period intermittent rotary.
7. the continuous automatic preparation facilities of the portable agar medium of substance containing temperature sensitive active of one kind according to claim 1,
It is characterized by: described automatically continuously pour into unit (6) include pouring into motor (601), peristaltic pump (602), input duct
(603), liquid outlet pipe (604), described to pour into motor (601) control peristaltic pump (602) imbibition drain, input duct (603) goes out
Liquid pipe road (604) is separately mounted to the liquid inlet and outlet of peristaltic pump (602), and input duct (603), liquid outlet pipe (604) are detachable
It and is high temperature high voltage resistant glass material.
8. the continuous automatic preparation facilities of the portable agar medium of substance containing temperature sensitive active of one kind according to claim 1,
It is characterized by: the sterilization unit (7) includes ultraviolet radiator (701), the ultraviolet radiator (701) is after starting to pour into
It is in close state.
9. a kind of continuous automatic preparation method of portable agar medium of substance containing temperature sensitive active, preparation process feature include with
Lower step:
S1: open back casing (102), the peristaltic pump that cleaning and sterilizing sterilizing-drying is disposed (602), input duct (603),
Liquid outlet pipe (604) is installed in equipment in an aseptic environment；
S2: pressing temperature control key, opens the work of equipment temperature control system, until displays temperature reaches setting temperature on display screen (304)
After degree, temperature control system, which automatically adjusts electric heating piece (403) temperature by temperature sensor (405), makes system remain constant temperature；
S3: the tank (401) equipped with agar solution after pressuresteam sterilization is installed, constant to required to temperature cooling
Thermostat temperature is added temperature sensitive substance from liquid outlet, then input duct (603) is connected, and presses the stirring on control panel (302)
Sterilize key, and starting automatic stirring tank (401) interior solution, ultraviolet radiator (701) is started to work simultaneously, and setting to be achieved is stirred
It mixes and sterilization time, is automatically stopped stirring work, ultraviolet radiator (701) also simultaneously closes off；
S4: the plate that sterilization has been dried is placed on rotating circular disc (203) to open in four circular grooves and fix, and will turn
Dynamic disk (203) and push square plate (204) i.e. following institute's evolution fluted shaft line of working good be aligneds, then will push square plate (204) left side
Right side institute evolution slot is inserted into operating position along push guide rail and contacts with phase magnet (206)；
S5: it presses and pours into key and automatically continuously carry out pouring into for the agar medium solution of substance containing temperature sensitive active, setting to be achieved
The time is poured into, work is automatically stopped and pours into and finish, extraction push square plate (204), the culture dish poured under replacement
Repetition is poured into.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910162220.7A CN109810893B (en) | 2019-03-05 | 2019-03-05 | Portable continuous automatic preparation device and method for agar culture medium containing temperature-sensitive active substances |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910162220.7A CN109810893B (en) | 2019-03-05 | 2019-03-05 | Portable continuous automatic preparation device and method for agar culture medium containing temperature-sensitive active substances |
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| CN109810893A true CN109810893A (en) | 2019-05-28 |
| CN109810893B CN109810893B (en) | 2024-03-08 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112111401A (en) * | 2020-09-27 | 2020-12-22 | 杨明 | Stem cell culture solution preparation device |
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| CN103874759A (en) * | 2011-04-29 | 2014-06-18 | Bd克斯特拉有限公司 | Disc dispensing device, tubular container for use in such a disc dispensing device and method of dispensing discs |
| CN204474657U (en) * | 2015-03-03 | 2015-07-15 | 浙江大学 | Portable agar plate pours into device |
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| GB835354A (en) * | 1957-04-01 | 1960-05-18 | Getinge Mek Verkst S Aktiebola | Improvements in or relating to incubation plants |
| CN103874759A (en) * | 2011-04-29 | 2014-06-18 | Bd克斯特拉有限公司 | Disc dispensing device, tubular container for use in such a disc dispensing device and method of dispensing discs |
| CN204474657U (en) * | 2015-03-03 | 2015-07-15 | 浙江大学 | Portable agar plate pours into device |
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| CN112111401A (en) * | 2020-09-27 | 2020-12-22 | 杨明 | Stem cell culture solution preparation device |
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| Publication number | Publication date |
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| CN109810893B (en) | 2024-03-08 |
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