CN109806441A - A kind of artificial heart printing bionical construction method of 3D printing equipment and artificial heart - Google Patents

Abstract

The invention discloses a kind of artificial heart printing 3D printing equipment and the bionical construction methods of artificial heart, a kind of bionical construction method of artificial organs, the artificial organs includes artificial organs thin-walled, bionical blood vessel fluid channel, cell tissue structure sheaf and bionic extracellular matrix, and the bionical construction method of the artificial organs is as follows: S1: hospital's image scanning；S2: pathological analysis is carried out according to the scanning information of acquisition；S3: to building referring to the carry out model layers processing of STL model and comparison STL model；S4: discrete manufactured slice of data is input in 3D biometric print machine；S5:3D printing；S6: organ culture environment is built.The invention also discloses the 3D printing equipment for realizing this method.

Description

A kind of artificial heart printing bionical building of 3D printing equipment and artificial heart Method

Technical field

The present invention relates to histoorgan field of engineering technology more particularly to a kind of artificial heart printing with 3D printing equipment with And the bionical construction method of artificial heart.

Background technique

Histoorgan engineering be using cell culture technique in vitro manual control cell differentiation, be proliferated and grow into need The tissue wanted, and be allowed to be engineered batch output, for repairing or repairing the body of the forfeiture of the function due to caused by accidental injury etc. Inner tissue meets clinical and rehabilitation its cardiac of needs as one of organ important in human body, once lesion, nothing occurs Method is retrieved, and the disease of heart class is very big threat to people's health life.

3D Organ printing instrument can be used in modern medicine, by constructing the three dimensional computer modeling of organ, utilizes three-dimensional Printing technique constructs active structure by manipulation cell and bio-ink, assembles living organic, but general organ constructs All only it can make single normal biological sample, but the organ that damages of patient itself and normal organ are there are different, it is direct right Patient carries out Living organ transplantation, not can guarantee transplanting safety, and without reference to group, there are unicity for experiment or operating result.

Summary of the invention

The purpose of the present invention is to solve disadvantages existing in the prior art, such as: general organ construction all can only be made Make single normal biological sample, without reference to group, there are unicity for experiment or operating result, and a kind of artificial device proposed The bionical construction method of official and the 3D printing equipment for realizing this method.

To achieve the goals above, present invention employs following technical solutions:

A kind of bionical construction method of artificial organs, the artificial organs include artificial organs thin-walled, bionical blood vessel fluid channel, The bionical construction method of cell tissue structure sheaf and bionic extracellular matrix, the artificial organs is as follows:

S1: hospital's image scanning, for acquiring the image information of the specific situation of patient's corresponding organ, using hospital CT, The equipment such as MRI, B ultrasound carry out tomoscan, obtain the fault information of corresponding organ, and carry out 3 D stereo structure to scanning information It builds, using medical scanning equipment, the organ structure of patient is scanned to come, then construct the later period further according to the actual conditions of patient Model；

S2: carrying out pathological analysis according to the scanning information of acquisition, for analyzing the pathologic condition of patient's organ, uses MIMICS software establishes patient organ STL model i.e. referring to STL model, while constructing the three-dimensional stereo model of normal organ i.e. pair Than STL model；

S3: to building referring to STL model and comparison STL model carry out model layers processing, according to cell itself structure, Bionics characteristic, be layered to model discrete using computer picture ancillary technique, is fabricated to multiple slices, and careful sets Count stacked system；

S4: discrete manufactured slice of data is input in 3D biometric print machine, living biological cell and bio-ink are used It is printed, selection is using the epimatrix environment with good bioactivity, similar internal cell, using the side coaxially printed Neural stem cell is done the same cell line of imaging line by method, and the center of line is neural stem cell, and what is be around wrapped in is then nerve Support cell, construction manual imitation organ thin-walled and angiogenic fluid channel first, centered on bionical blood vessel fluid channel successively Cell tissue structure sheaf is constructed, after superposition bonds artificial organs thin-walled marginal position, adds internal bionic extracellular matrix, suture Artificial organs manufacture finishes；

S5: using 3D biometric print machine, prints reference cell slice, uses methacrylated water-setting in print procedure Glue (GelMA) carries out pasting between cell, finally pastes to form complete entity referring to organ, similarly prints comparison cell Slice, and comparison organ model is bonded into using GelMA；

S6: organ culture environment is built, and constructs biotic environment according to the actual demand of sufferer, by comparison organ and reference device Official places saves culture in the medium, observes and records physiological change.

Preferably, the bio-ink is made of gelatin and sodium alginate, and the living cells is to extract to do from patient's body Cell, primordial stem cell are stored among subzero 196 degree of liquid nitrogen, and the living cells that the later period uses is differentiated by stem cell Living cells.

Preferably, the reference organ is with comparison organ culture and observation differs for 1 day to 1 month, records surviving for cell The biological characteristics such as rate, mechanical stability, functionality, bionical characteristic, the culture environment temperature control use sterile behaviour at 37 DEG C Make case.

A kind of 3D printing equipment for realizing the bionical construction method of artificial organs, the 3D printing equipment, including 3D biology are beaten Print machine, which is characterized in that be equipped with liquor box in the 3D biometric print machine, be provided with skate machine in the 3D biometric print machine The inner wall of structure, the sliding equipment and 3D biometric print machine is slidably connected, and multiple cylinders are equipped on the slide construction, described The driving end of cylinder is fixedly connected with telescopic shaft, and multiple telescopic shafts are fixedly connected with fixed frame far from one end of cylinder, institute It states and is rotatably connected to the first ball screw in fixed frame, printing driving machine is slidably connected on first ball screw, it is described Printing driving machine in mobile mechanism is installed, be provided in the printing driving machine gel storage box, histocyte the storage box and The upper end of stem cell the storage box, the gel storage box, histocyte the storage box and stem cell the storage box is respectively connected with input pipe, Gel efferent duct is connected with below the gel storage box, it is defeated to be connected with histocyte below the histocyte the storage box Outlet pipe is connected with stem cell efferent duct below the stem cell the storage box, holder is connected in the liquor box, described molten The two sides of liquid case are symmetrically inserted with solution conduit, offer diversion trench on the inner wall of the liquor box.

Preferably, the sliding equipment includes top plate, and the two sides of the top plate are symmetrically fixedly connected with support shaft, the branch The lower end of support axis is slidably connected with 3D biometric print motor spindle inner wall, and sliding electricity is symmetrically installed in the 3D biometric print machine Machine, the driving end of the sliding motor are fixedly connected with the second ball screw, and is equipped in the lower end of two support shafts Two nuts, second nut are socketed on the second ball screw.

Preferably, the mobile mechanism includes a pair of first nut, is equipped with mobile motor in the printing driving machine, institute The driving end for stating mobile motor is fixedly connected with worm screw, and tooth socket, two teeth are offered on the outside of two first nuts Slot engagement is connected with gear, and two gears are fixedly connected by universal driving shaft, and fixing sleeve is connected to worm gear on the universal driving shaft, institute Worm gear is stated to engage with worm screw.

Preferably, the solution conduit is fixedly connected with baffle close to one end of liquor box, opens up on the downside of the solution conduit There is opening.

Preferably, card slot is offered on the holder, the holder is clamped by card slot and liquor box, the placement Box is honeycomb engraved structure.

It compared with prior art, can the beneficial effects of the present invention are: the bio-ink formed using gelatin and sodium alginate To control the retardance of biometric print by temperature, further develop convenient for the cell after printing to establish between cell Communication, guarantees that each cell is connected between each other；

By designing control group, guarantees to be observed during culture, can clearly be observed as referring to experiment Pathological change can use the diversity of experiment sample, do and transplant pre- reality in vitro as the reference body of later period drug therapy It tests, is further ensured that experiment safety.

Detailed description of the invention

Fig. 1 is a kind of flow chart of the bionical construction method of artificial organs proposed by the present invention；

Fig. 2 is the structural schematic diagram that 3D printing equipment is used in a kind of artificial organs printing proposed by the present invention；

Fig. 3 is the structural representation that driving machine is printed in a kind of artificial organs printing 3D printing equipment proposed by the present invention Figure；

Fig. 4 is the structural schematic diagram of mobile mechanism in a kind of artificial organs printing 3D printing equipment proposed by the present invention；

Fig. 5 is the structural schematic diagram in Fig. 2 at A.

In figure: 1 support shaft, 2 fixed frames, 3 cylinders, 4 top plates, 5 telescopic shafts, 6 3D biometric print machines, 7 solution conduits, 8 gels Efferent duct, 9 histocyte efferent ducts, 10 stem cell efferent ducts, 11 printing driving machines, 12 first ball screws, 13 liquor boxs, 14 Diversion trench, 15 sliding motors, 16 second nuts, 17 card slots, 18 holders, 19 second ball screws, 20 openings, 21 baffles, 22 Stem cell the storage box, 23 histocyte the storage box, 24 gel storage boxes, 25 input pipes, 26 worm screws, 27 mobile motors, 28 gears, 29 universal driving shafts, 30 worm gears, 31 first nuts, 32 tooth sockets.

Specific embodiment

Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.

In the description of the present invention, it is to be understood that, term " on ", "lower", "front", "rear", "left", "right", "top", The orientation or positional relationship of the instructions such as "bottom", "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings, merely to just In description the present invention and simplify description, rather than the device or element of indication or suggestion meaning must have a particular orientation, with Specific orientation construction and operation, therefore be not considered as limiting the invention.

Embodiment 1

Artificial organs is by taking artificial heart as an example, referring to Fig.1-5, a kind of artificial organs printing 3D printing equipment, including 3D Biometric print machine 6 is equipped with liquor box 13 in 3D biometric print machine 6, is provided with sliding equipment in 3D biometric print machine 6, slides Mechanism and the inner wall of 3D biometric print machine 6 are slidably connected, and multiple cylinders 3 are equipped on slide construction, and the driving end of cylinder 3 is fixed It is connected with telescopic shaft 5, multiple telescopic shafts 5 are fixedly connected with fixed frame 2 far from one end of cylinder 3, are rotatably connected in fixed frame 2 First ball screw 12 slidably connects printing driving machine 11 on first ball screw 12, prints in driving machine 11 and be equipped with shifting Motivation structure prints in driving machine 11 and is provided with gel storage box 24, histocyte the storage box 23 and stem cell the storage box 22, gel The upper end of the storage box 24, histocyte the storage box 23 and stem cell the storage box 22 is respectively connected with input pipe 25, gel storage box 24 Lower section be connected with gel efferent duct 8, the lower section of histocyte the storage box 23 is connected with histocyte efferent duct 9, and stem cell is deposited The lower section of storage box 22 is connected with stem cell efferent duct 10, and holder 18 is connected in liquor box 13, and the two sides of liquor box 13 are symmetrical It is inserted with solution conduit 7, diversion trench 14 is offered on the inner wall of liquor box 13.

Wherein, sliding equipment includes top plate 4, and the two sides of top plate 4 are symmetrically fixedly connected with support shaft 1, the lower end of support shaft 1 It is slidably connected with 6 bottom interior wall of 3D biometric print machine, is symmetrically installed with sliding motor 15, sliding motor in 3D biometric print machine 6 15 driving end is fixedly connected with the second ball screw 19, and the second nut 16, the second spiral shell are equipped in the lower end of two support shafts 1 Mother 16 is socketed on the second ball screw 19, when two sliding motors 15 drive the rotation of the second ball screw 19 by driving end When, the rotation of the second ball screw 19 can realize that opposite second ball screw 19 of the second nut 16 slides, to realize support The movement of axis 1, support shaft 1 drive the movement of entire printing driving machine 11 by top plate 4, realize axial direction in the horizontal plane It is mobile；

Wherein, mobile mechanism includes a pair of first nut 31, prints and is equipped with mobile motor 27 in driving machine 11, mobile electricity The driving end of machine 27 is fixedly connected with worm screw 26, and the outside of two the first nuts 31 offers tooth socket 32, and two tooth sockets 32 engage It is connected with gear 28, two gears 28 are fixedly connected by universal driving shaft 29, and fixing sleeve is connected to worm gear 30, worm gear 30 on universal driving shaft 29 It is engaged with worm screw 26, mobile motor 27 drives worm screw 26 to rotate by driving end, and worm screw 26 engages with universal driving shaft 30, universal driving shaft 30 Two mobile motors 28 are driven to rotate synchronously by universal driving shaft 29, mobile motor 28 is engaged with tooth socket 32, then 28 band of mobile motor Dynamic universal driving shaft 31 rotates synchronously, and universal driving shaft 31 is rotated relative to the first ball screw 12, converts linear motion for rotary motion, real Now printing driving machine 11 another axial movement in the horizontal plane, then sliding equipment and mobile mechanism cooperate, can be in plane On move freely, realize cell printing layer by layer.

Wherein, solution conduit 7 is fixedly connected with baffle 21 close to one end of liquor box 13, and the downside of solution conduit 7 offers out Mouth 20, when bio-ink is input in liquor box 13 by solution conduit 7, due to the blocking of baffle 21, so that biology ink Water can only go downstream along diversion trench 14 by 20 output of opening, avoid splashing of bio-ink during gradually exporting, The printing of heart is influenced, card slot 17 is offered on holder 18, holder 18 is clamped by card slot 17 and liquor box 13, holder 18 be honeycomb engraved structure, and for card slot 17 to fixed placement box 18, holder 18 is to come out according to patient's heart profile scan Mechanism, the heart to print provides the structural support, and avoids the inclination of angle position, while the structure of hollow out, guarantees the heart The dirty moment is immersed among bio-ink, guarantees the excellent activity of cell.

In the present invention, preset heart printing step is inputted, artificial organs is that the mutual place mat of cell from level to level is pasted, most It is sutured afterwards by artificial organs thin-walled, when type body, trunk nerve at the printing first of stem cell efferent duct 10, Then it is tiled using trunk nerve as axle center using histocyte efferent duct 9, i.e., in central print neural stem cell, on periphery Printing support cell, that is, histocyte, first construction manual imitation heart thin-walled and angiogenic fluid channel, with bionical blood vessel miniflow Cell tissue structure sheaf is successively constructed centered on road, the gel that cell passes through the output of gel efferent duct 8 between each other is bonded Fixed, wherein printed material is input to each gel storage box 24, histocyte the storage box 23 and stem cell by input pipe 25 In the storage box 22, during printing, bio-ink will be gradually inputted in solution conduit 7, bio-ink is impregnated always beats The tissue come is printed off, guarantees the primary activity of cell；

Cell place mat from level to level is formed due to being, so entirely printing driving machine 11 needs freely to live with three-dimensional space Dynamic ability then prints driving machine 11 and moves up and down by the driving telescopic shaft 5 of cylinder 3, realizes fixed frame 2 and printing driving machine 11 move up and down, and sliding motor 15 drives the rotation of the second ball screw 19, and second ball screw 19 is real by the second nut 16 The movement of the movement of existing support shaft 1 axial direction in the horizontal plane, i.e. printing driving machine 11 axial direction in the horizontal plane, mobile electricity Machine 27 drives universal driving shaft 30 to rotate by worm screw 26, and universal driving shaft 30 drives mobile motor 28 to rotate by universal driving shaft 29, mobile electricity Machine 28 is engaged with tooth socket 32, and the rotation of universal driving shaft 31 will will drive printing driving machine 11 relative to the movement of the first ball screw 12, Then another the axial movement in the horizontal plane of printing driving machine 11 is realized.

A kind of bionical construction method of artificial organs, the artificial heart include artificial heart thin-walled, bionical blood vessel fluid channel, The bionical construction method of cell tissue structure sheaf and bionic extracellular matrix, the artificial heart is as follows:

S1: hospital's image scanning, the image information of the specific situation of acquisition patient's corresponding organ (heart), using hospital CT, The equipment such as MRI, B ultrasound carry out tomoscan, obtain the fault information of corresponding organ, and carry out 3 D stereo structure to scanning information It builds, using medical scanning equipment, the organ structure of patient is scanned to come, then construct the later period further according to the actual conditions of patient Model；

S2: carrying out pathological analysis according to the scanning information of acquisition, analyzes the pathologic condition of patient's organ, soft using MIMICS Part establishes patient organ STL model i.e. referring to STL model, while the three-dimensional stereo model for constructing normal organ compares STL mould Type, although human organ is unanimous on the whole, personal specific physiological status is had differences, and needs the self-demand according to patient The corresponding normal STL model of building, while also needing to retain the STL model under patient's standing state as a comparison；

S3: to building referring to STL model and comparison STL model carry out model layers processing, according to cell itself structure, Bionics characteristic be layered to model discrete, to cell type, cell tissue structure using computer picture ancillary technique Level, the shape of cell, hereditary information of Cell Age, cell etc. carry out bioanalysis, are fabricated to multiple slices, and thin Cause design stacked system, 3D biometric print technology is to be integrated by the connecting method of retroversion, so firstly the need of by organ Careful discrete and analysis is inside carried out in a organized way, and formulates careful superposition bonding mode；

S4: discrete manufactured slice of data is input in 3D biometric print machine, living biological cell and bio-ink are used It is printed, selection is using the epimatrix environment with good bioactivity, similar internal cell, such as gelatin and sodium alginate The bio-ink of composition, this bio-ink have the characteristic changed with temperature, can control biometric print by temperature Retardance is further developed convenient for the cell after printing, to establish the communication between cell, using the side coaxially printed Neural stem cell is done the same cell line of imaging line by method, and the center of line is neural stem cell, and what is be around wrapped in is then nerve Support cell, enable these cells to be constantly be generated trophic factors, the mode coaxially printed, i.e., central print mind Through stem cell, support cell, first construction manual imitation heart thin-walled and angiogenic fluid channel are printed on periphery, with bionical blood vessel Cell tissue structure sheaf is successively constructed centered on fluid channel, after superposition bonds artificial heart thin-walled marginal position, is added internal imitative Raw extracellular matrix, suture artificial heart manufacture finish, and then the mode coaxially printed wraps up bar rope, this state The survival rate of lower fiber bundle cell is quite high, and has released nerve growth factor to a certain extent；

S5: using 3D biometric print machine, prints reference cell slice, uses methacrylated water-setting in print procedure Glue (GelMA) carries out pasting between cell, and cell can be proliferated stretching, extension and be migrated in GelMA, finally pastes to be formed completely Entity similarly print comparison cell section referring to organ, and be bonded into comparison organ model, 3D biology using GelMA Using living cells, place mat, cell from level to level pass through GelMA between each other and are bonded printer from level to level, can just be spliced into one A complete organ structure；

S6: organ culture environment is built, and constructs biotic environment according to the actual demand of sufferer, by comparison organ and reference device Official places saves culture in the medium, observes and records physiological change.

The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto, Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.

Claims (8)

1. the bionical construction method of a kind of artificial heart printing 3D printing equipment and artificial heart, which includes people Work organ thin-walled, bionical blood vessel fluid channel, cell tissue structure sheaf and bionic extracellular matrix, which is characterized in that the artificial device The bionical construction method of official is as follows:

S1: hospital's image scanning uses hospital CT, MRI, B ultrasound for acquiring the image information of the specific situation of patient's corresponding organ Etc. equipment carry out tomoscan, obtain the fault information of corresponding organ, and 3 D stereo building is carried out to scanning information, use Medical scanning equipment scans the organ structure of patient to come, then further according to the model in the actual conditions building later period of patient；

S2: carrying out pathological analysis according to the scanning information of acquisition, soft using MIMICS for analyzing the pathologic condition of patient's organ Part establishes patient organ STL model i.e. referring to STL model, while the three-dimensional stereo model for constructing normal organ compares STL mould Type；

S3: to building referring to the carry out model layers processing of STL model and comparison STL model, according to cell itself structure, bionical Characteristic is learned, model be layered using computer picture ancillary technique discrete, is fabricated to multiple slices, and Careful design is folded Add mode；

S4: discrete manufactured slice of data is input in 3D biometric print machine, is carried out using living biological cell and bio-ink Printing, selection is using the epimatrix environment with good bioactivity, similar internal cell, using the method coaxially printed, Neural stem cell is done the same cell line of imaging line, the center of line is neural stem cell, and what is be around wrapped in is then neural Support cell, first construction manual imitation organ thin-walled and angiogenic fluid channel, the layer-by-layer structure centered on bionical blood vessel fluid channel Cell tissue structure sheaf is built, after superposition bonds artificial organs thin-walled marginal position, internal bionic extracellular matrix is added, sutures people The manufacture of work organ finishes；

S5: using 3D biometric print machine, prints reference cell slice, uses methacrylated hydrogel in print procedure (GelMA) pasting between cell is carried out, finally pastes to form complete entity referring to organ, similarly prints comparison cell and cut Piece, and comparison organ model is bonded into using GelMA；

S6: organ culture environment is built, and is constructed biotic environment according to the actual demand of sufferer, is put by comparison organ and referring to organ It sets and saves culture in the medium, observe and record physiological change.

2. the bionical construction method of a kind of artificial organs according to claim 1, which is characterized in that the bio-ink is by bright Glue and sodium alginate composition, the living cells are to extract stem cell from patient's body, and primordial stem cell is stored in subzero 196 degree Among liquid nitrogen, the living cells that the later period uses is the living cells differentiated by stem cell.

3. the bionical construction method of a kind of artificial organs according to claim 2, which is characterized in that described referring to organ and right Than organ culture and observation differs for 1 day to 1 month, records survival rate, mechanical stability, functionality, bionical characteristic of cell etc. Biological characteristic, the culture environment temperature control use aseptic operation box at 37 DEG C.

4. a kind of 3D printing equipment for realizing the bionical construction method of artificial organs, it is characterised in that: the 3D printing equipment, including 3D biometric print machine (6), which is characterized in that be equipped with liquor box (13) in the 3D biometric print machine (6), the 3D biology is beaten It is provided with sliding equipment in print machine (6), the inner wall of the sliding equipment and 3D biometric print machine (6) is slidably connected, the sliding It is equipped in structure multiple cylinders (3), the driving end of the cylinder (3) is fixedly connected with telescopic shaft (5), multiple telescopic shafts (5) one end far from cylinder (3) is fixedly connected with fixed frame (2), is rotatably connected to the first ball screw in the fixed frame (2) (12), printing driving machine (11), installation in the printing driving machine (11) are slidably connected on first ball screw (12) There is mobile mechanism, gel storage box (24), histocyte the storage box (23) are provided in the printing driving machine (11) and do thin The upper end of born of the same parents' the storage box (22), the gel storage box (24), histocyte the storage box (23) and stem cell the storage box (22) is equal It is connected with input pipe (25), is connected with gel efferent duct (8) below the gel storage box (24), the histocyte storage It is connected with below box (23) histocyte efferent duct (9), it is defeated to be connected with stem cell below the stem cell the storage box (22) Outlet pipe (10), the liquor box (13) is interior to be connected with holder (18), and the two sides of the liquor box (13) are symmetrically inserted with solution It manages (7), offers diversion trench (14) on the inner wall of the liquor box (13).

5. a kind of 3D printing equipment for realizing the bionical construction method of artificial organs according to claim 4, which is characterized in that The sliding equipment includes top plate (4), and the two sides of the top plate (4) are symmetrically fixedly connected with support shaft (1), the support shaft (1) lower end is slidably connected with 3D biometric print machine (6) bottom interior wall, is symmetrically installed with cunning in the 3D biometric print machine (6) Dynamic motor (15), the driving end of the sliding motor (15) are fixedly connected with the second ball screw (19), two support shafts (1) it is equipped in lower end the second nut (16), second nut (16) is socketed on the second ball screw (19).

6. a kind of 3D printing equipment for realizing the bionical construction method of artificial organs according to claim 5, which is characterized in that The mobile mechanism includes a pair of first nut (31), is equipped with mobile motor (27) in the printing driving machine (11), described The driving end of mobile motor (27) is fixedly connected with worm screw (26), offers tooth socket on the outside of two first nuts (31) (32), two tooth socket (32) engagements are connected with gear (28), and two gears (28) connect by the way that universal driving shaft (29) are fixed It connects, fixing sleeve is connected to worm gear (30) on the universal driving shaft (29), and the worm gear (30) is engaged with worm screw (26).

7. a kind of 3D printing equipment for realizing the bionical construction method of artificial organs according to claim 6, which is characterized in that The solution conduit (7) is fixedly connected with baffle (21) close to the one end of liquor box (13), offers on the downside of the solution conduit (7) It is open (20).

8. a kind of 3D printing equipment for realizing the bionical construction method of artificial organs according to claim 7, which is characterized in that It is offered card slot (17) on the holder (18), the holder (18) is clamped by card slot (17) and liquor box (13), institute Stating holder (18) is honeycomb engraved structure.