CN110229740A - A kind of Biotype artificial liver cell 3D printing system - Google Patents
A kind of Biotype artificial liver cell 3D printing system Download PDFInfo
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- CN110229740A CN110229740A CN201910517512.8A CN201910517512A CN110229740A CN 110229740 A CN110229740 A CN 110229740A CN 201910517512 A CN201910517512 A CN 201910517512A CN 110229740 A CN110229740 A CN 110229740A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y30/00—Apparatus for additive manufacturing; Details thereof or accessories therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
Abstract
The present invention provides a kind of Biotype artificial liver cell 3D printing system, including printing mechanism, stamp pad, control system, the control system passes through route respectively and is connected with the printing mechanism with stamp pad；The printing mechanism includes mobile unit, print unit, support, the mobile unit is fixed on the support, the print unit is connected with the mobile unit, and the mobile unit, print unit pass through route respectively and be connected with the control system.For Biotype artificial liver of the present invention with cell 3D printing system by mobile unit, print unit, stamp pad, the cooperation of control system improves accuracy, the stability of printing, can accurately control individual cells and extracellular matrix distribution.
Description
Technical field
The invention belongs to cell printing fields, more particularly, to a kind of Biotype artificial liver cell 3D printing system.
Background technique
Artificial liver refers to by an external machinery, physics and chemistry or bioreactor device, removes because hepatic failure produces
Raw or increased various harmful substances, the necessary substance such as protein that supplement needs liver to synthesize or be metabolized, improve patient's water, electrolysis
Environment in matter and acid-base balance etc. temporarily assists or substitutes the corresponding major function of liver, until self liver cell regeneration, liver function
It can restore, or improve the symptom of end-stage liver disease patient, become " bridge " of liver transfer operation, improve survival.It is referred to as " artificial
Liver ".
Liver failure is the severe stage of various hepatopathy development, and case fatality rate is high, there is no effective treatment method at present.People
Work liver system is to treat one of the important method of liver failure comprising Non-biotype artificial liver, Biotype artificial liver
And hybrid artificial liver.Currently, Biotype artificial liver is closest to human liver's removing toxic substances, synthesizes, the artificial liver of metabolic function dress
It sets.Due to liver function complexity, fill Biotype artificial liver in the screening of seed cell, the building of bioreactor and with auxiliary
Larger challenge is faced in terms of the optimal combination set, Biotype artificial liver is caused to develop slowly.
Cell printing technology is a kind of new technology of the combination of rapid shaping technique and Biotechnology.Section at present
Scholar has developed the three dimensional biological printer that can print skin, cartilage, bone and body other organs.Cell printing work
Skill, as shown in Figure 1.Cell printing technology receives the extensive concern of scholars.Biometric print machine facing challenges first is that
The human body of many cells composition or the organ of animal are printed, because any organ printed or bodily tissue require same body
Blood vessel be connected.Current biometric print technology is mainly to study new molding mode, cell scaffold material, improves molding
The research of precision and shaped support mechanical performance etc..
Cell printing technology is varied, can be divided into ink jet type, injecting type, induced with laser direct write, laser by printing type
Induction transfer, laser curing and acoustic control printing.Current biometric print technology is in building bulk soft tissue and internal organs side
Face, there are still many shortcoming: 1) cell density planted is limited, and cambium is grown into slowly；2) with it is three-dimensional in natural tissues
Balanced living environment is different, and cell is internally grown by rack surface, thus is unfavorable for the contacting with each other of cell, corresponding organ
Development and functional expression；3) vascularization is insufficient, without good vascularization solution, causes nutriment in internal stent
Penetrating power reduces, and cell cannot get sufficient nutrient, thus in soft group of bulk for also cannot achieve bulk and needing blood supply abundance
It knits and internal organs；4) it cannot achieve different cellular localization to different spatial positions, and bulk soft tissue and interior internal organs
Official often contains various kinds of cell, and different cells have the characteristics that distinctive spatial arrangement.
Cell is as the basic unit for constituting human organ, and size is in the range of several microns to tens microns.Therefore,
How accurately to control the distribution of different types of cell and extracellular matrix on three dimension scale, and is formed and human body or animal
Tissue or the similar three-dimensional tectosome of organ be a great problem that organizational project is faced.
Summary of the invention
In view of this, the present invention is directed to propose a kind of Biotype artificial liver cell 3D printing system, printing are accurate, steady
It is fixed.
In order to achieve the above objectives, the technical scheme of the present invention is realized as follows:
A kind of Biotype artificial liver cell 3D printing system, including printing mechanism, stamp pad, control system, it is described
Control system passes through route respectively and is connected with the printing mechanism with stamp pad；
The printing mechanism includes mobile unit, print unit, support, and the mobile unit is fixed on the branch
On seat, the print unit is connected with the mobile unit, the mobile unit, print unit pass through respectively route with
The control system is connected；
The mobile unit include the first supporting element, rack gear, litter, the second supporting element, driving gear, servo motor,
Fixing sleeve, mobile box, the mobile box are fixed on the support, and first supporting element is fixed on described
In mobile box, the middle part of first supporting element is equipped with the shifting chute of perforation, and one end of the rack gear is placed in described
In shifting chute, the other end is connected with the litter, and second supporting element is connected with the mobile box, the cunning
Pole passes through second supporting element and is connected with the fixing sleeve, and the fixing sleeve is placed on the outer of the print unit
Side, the driving gear are meshed with the rack gear, and are connected with the servo motor, and the servo motor passes through
Route is connected with the control system.
Further, the print unit include outer sleeve, it is inner sleeve, check valve, inner nozzle, fixed disk, fixing seat, outer
Nozzle, the outer sleeve are fixed in the fixing sleeve, and the inner sleeve is fixed in the outer sleeve, described
One end of check valve is connected with the inner sleeve, and the other end is connected with across the fixed disk with the inner nozzle, institute
The check valve stated is connected by route with the control system, and the fixed seating is in the lower section of the inner nozzle, institute
The one end for the outer nozzle stated is connected with the inner nozzle, and the other end passes through the fixed seating in outside the outer sleeve.
Further, the stamp pad includes table top, cell container, support frame, saddle, sliding rail, the support frame
One end be connected with the saddle, the other end is connected with the table top, and the cell container is fixed on the platform
On face, the saddle is matched with the sliding rail, and the controller is connected by route with the saddle.
Further, the cell container is rectangular configuration.
Further, the sliding rail is mutually perpendicular to the rack gear.
Using the Biotype artificial liver Method of printing of cell 3D printing system, include the following steps:
(1) bio-ink is configured, cell will wherein be added, preparation obtains bio-ink liquid containing cell；
(2) bat nozzle is placed in the surface of one interior angle of cell container；
(3) by the bio-ink liquid injection inner sleeve, control system controls check valve and opens, bio-ink liquid
Body flows into inner nozzle, is then printed by outer nozzle；
(4) control system control servo motor is opened, and is realized and is rotated forward, drives the rotation of driving gear, pass through driving gear
With engaging for rack gear, the movement of litter is pulled, and then print unit is driven to move along a straight line, completes beating for first row cell
Print；
(5) position of the mobile cell factory of control system driving saddle, then control system control servo motor is anti-
Turn, print unit is driven to move along a straight line, completes the printing of secondary series cell；
(6) repetitive cycling step (4) and step (5) are until complete the printing of last column cell.
Further, in the step (5) cell factory distance be more than or equal to a cell diameter.
Bio-ink in the step (1) is by including that the component of following parts by weight is made:
Further, the hydrophilic agent is one of chitosan or gelatin.
Further, the chaotropic agent is one of acetic acid, hydrochloric acid, nitrous acid or phosphoric acid.
Compared with the existing technology, Biotype artificial liver of the present invention is had the advantage that with cell 3D printing system
(1) Biotype artificial liver of the present invention passes through mobile unit, print unit, printing with cell 3D printing system
Platform, the cooperation of control system improve accuracy, the stability of printing, can accurately control individual cells and extracellular matrix
Distribution.
(2) bio-ink of the present invention improves hydrogel intensity, promotes adherency, proliferation and the differentiation of cell, nothing
Poison is pollution-free, can wrap up cell and carry out 3D printing.
Detailed description of the invention
The attached drawing for constituting a part of the invention is used to provide further understanding of the present invention, schematic reality of the invention
It applies example and its explanation is used to explain the present invention, do not constitute improper limitations of the present invention.In the accompanying drawings:
Fig. 1 is the schematic diagram of Biotype artificial liver cell 3D printing system described in the embodiment of the present invention；
Fig. 2 is the schematic diagram of print unit described in the embodiment of the present invention.
Description of symbols:
1- printing mechanism；2- stamp pad；3- control system；11- support；12- mobile unit；13- print unit；121-
One supporting element；1211- shifting chute；122- rack gear；123- litter；The second supporting element of 124-；125- driving gear；126- servo electricity
Machine；127- fixing sleeve；128- mobile box；131- outer sleeve；132- inner sleeve；133- check valve；134- inner nozzle；135- is solid
Price fixing；136- fixing seat；137- outer nozzle；21- table top；22- cell container；23- support frame；24- saddle；25- sliding rail.
Specific embodiment
It should be noted that in the absence of conflict, the feature in embodiment and embodiment in the present invention can phase
Mutually combination.
In the description of the present invention, it is to be understood that, term " center ", " longitudinal direction ", " transverse direction ", "upper", "lower",
The orientation or positional relationship of the instructions such as "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outside" is
It is based on the orientation or positional relationship shown in the drawings, is merely for convenience of description of the present invention and simplification of the description, rather than instruction or dark
Show that signified device or element must have a particular orientation, be constructed and operated in a specific orientation, therefore should not be understood as pair
Limitation of the invention.In addition, term " first ", " second " etc. are used for description purposes only, it is not understood to indicate or imply phase
To importance or implicitly indicate the quantity of indicated technical characteristic.The feature for defining " first ", " second " etc. as a result, can
To explicitly or implicitly include one or more of the features.In the description of the present invention, unless otherwise indicated, " multiple "
It is meant that two or more.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation ", " phase
Even ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or be integrally connected；It can
To be mechanical connection, it is also possible to be electrically connected；It can be directly connected, can also can be indirectly connected through an intermediary
Connection inside two elements.For the ordinary skill in the art, above-mentioned term can be understood by concrete condition
Concrete meaning in the present invention.
The present invention will be described in detail below with reference to the accompanying drawings and embodiments.
Embodiment 1
A kind of Biotype artificial liver cell 3D printing system, including printing mechanism 1, stamp pad 2, control system 3, it is described
Control system 3 be connected respectively with stamp pad 2 with the printing mechanism 1 by route；
The printing mechanism 1 includes mobile unit 12, print unit 13, support 11, and the mobile unit 12 is fixed
In on the support 11, the print unit 13 is connected with the mobile unit 12, the mobile unit 12, printing
Unit 13 is connected by route with the control system 3 respectively；
The mobile unit 12 includes the first supporting element 121, rack gear 122, litter 123, the second supporting element 124, active
Gear 125, servo motor 126, fixing sleeve 127, mobile box 128, the mobile box 128 are fixed on the support 11
On, first supporting element 121 is fixed in the mobile box 128, and the middle part of first supporting element 121 is equipped with
The shifting chute 1211 of perforation, one end of the rack gear 122 are placed in the shifting chute 1211, the other end and the litter
123 are connected, and second supporting element 124 is connected with the mobile box 128, and the litter 123 is across described the
Two supporting elements 124 are connected with the fixing sleeve 127, and the fixing sleeve 127 is placed on the outside of the print unit 13, institute
The driving gear 125 stated is meshed with the rack gear 122, and is connected with the servo motor 126, the servo motor
126 are connected by route with the control system 3.
The print unit 13 includes outer sleeve 131, inner sleeve 132, check valve 133, inner nozzle 134, fixed disk
135, fixing seat 136, outer nozzle 137, the outer sleeve 131 are fixed in the fixing sleeve 127, the inner sleeve
132 are fixed in the outer sleeve 131, and one end of the check valve 133 is connected with the inner sleeve 132, the other end
It is connected with the fixed disk 135 is passed through with the inner nozzle 134, the check valve 133 passes through route and the control
System 3 processed is connected, and the fixing seat 136 is located at the lower section of the inner nozzle 134, one end of the outer nozzle 137 and
The inner nozzle 134 is connected, and the other end passes through the fixing seat 136 and is located at outside the outer sleeve 131.
The stamp pad 2 includes table top 21, cell container 22, support frame 23, saddle 24, sliding rail 25, the branch
One end of support 23 is connected with the saddle 24, and the other end is connected with the table top 21, and the cell container 22 is solid
Due on the table top 21, the saddle 24 is matched with the sliding rail 25, and the controller passes through route and institute
The saddle 24 stated is connected.
Control system 3 is controlled using conventional control system 3, and the structure of driving saddle 24 is conventional structure, at this
Without detailed description in invention.
The cell container 22 is rectangular configuration.
The sliding rail 25 is mutually perpendicular to the rack gear 122.
Using the Biotype artificial liver Method of printing of cell 3D printing system, include the following steps:
(1) bio-ink is configured, cell will wherein be added, preparation obtains bio-ink liquid containing cell；
(2) bat nozzle is placed in the surface of 22 1 interior angles of cell container；
(3) by the bio-ink liquid injection inner sleeve 132, control system 3 controls check valve 133 and opens, raw
Object ink liquid flows into inner nozzle 134, is then printed by outer nozzle 137；
(4) control system 3 controls servo motor 126 and opens, and realizes and rotates forward, and drives the rotation of driving gear 125, passes through master
Moving gear 125 is engaged with rack gear 122, pulls the movement of litter 123, and then print unit 13 is driven to move along a straight line, complete
At the printing of first row cell；
(5) control system 3 drives the position of the mobile cell factory of saddle 24, and then control system 3 controls servo electricity
Machine 126 inverts, and print unit 13 is driven to move along a straight line, and completes the printing of secondary series cell；
(6) repetitive cycling step (4) and step (5) are until complete the printing of last column cell.
The distance of a cell factory is 1.2 times of the diameter of a cell in the step (5).
Bio-ink in the step (1) is by including that the component of following parts by weight is made: 2 parts of sodium alginate, shell is poly-
3 parts, 2 parts of acetic acid, 60 parts of water of sugar.
Embodiment 2
A kind of Biotype artificial liver cell 3D printing system, including printing mechanism 1, stamp pad 2, control system 3, it is described
Control system 3 be connected respectively with stamp pad 2 with the printing mechanism 1 by route；
The printing mechanism 1 includes mobile unit 12, print unit 13, support 11, and the mobile unit 12 is fixed
In on the support 11, the print unit 13 is connected with the mobile unit 12, the mobile unit 12, printing
Unit 13 is connected by route with the control system 3 respectively；
The mobile unit 12 includes the first supporting element 121, rack gear 122, litter 123, the second supporting element 124, active
Gear 125, servo motor 126, fixing sleeve 127, mobile box 128, the mobile box 128 are fixed on the support 11
On, first supporting element 121 is fixed in the mobile box 128, and the middle part of first supporting element 121 is equipped with
The shifting chute 1211 of perforation, one end of the rack gear 122 are placed in the shifting chute 1211, the other end and the litter
123 are connected, and second supporting element 124 is connected with the mobile box 128, and the litter 123 is across described the
Two supporting elements 124 are connected with the fixing sleeve 127, and the fixing sleeve 127 is placed on the outside of the print unit 13, institute
The driving gear 125 stated is meshed with the rack gear 122, and is connected with the servo motor 126, the servo motor
126 are connected by route with the control system 3.
The print unit 13 includes outer sleeve 131, inner sleeve 132, check valve 133, inner nozzle 134, fixed disk
135, fixing seat 136, outer nozzle 137, the outer sleeve 131 are fixed in the fixing sleeve 127, the inner sleeve
132 are fixed in the outer sleeve 131, and one end of the check valve 133 is connected with the inner sleeve 132, the other end
It is connected with the fixed disk 135 is passed through with the inner nozzle 134, the check valve 133 passes through route and the control
System 3 processed is connected, and the fixing seat 136 is located at the lower section of the inner nozzle 134, one end of the outer nozzle 137 and
The inner nozzle 134 is connected, and the other end passes through the fixing seat 136 and is located at outside the outer sleeve 131.
The stamp pad 2 includes table top 21, cell container 22, support frame 23, saddle 24, sliding rail 25, the branch
One end of support 23 is connected with the saddle 24, and the other end is connected with the table top 21, and the cell container 22 is solid
Due on the table top 21, the saddle 24 is matched with the sliding rail 25, and the controller passes through route and institute
The saddle 24 stated is connected.
The cell container 22 is rectangular configuration.
The sliding rail 25 is mutually perpendicular to the rack gear 122.
Using the Biotype artificial liver Method of printing of cell 3D printing system, include the following steps:
(1) bio-ink is configured, cell will wherein be added, preparation obtains bio-ink liquid containing cell；
(2) bat nozzle is placed in the surface of 22 1 interior angles of cell container；
(3) by the bio-ink liquid injection inner sleeve 132, control system 3 controls check valve 133 and opens, raw
Object ink liquid flows into inner nozzle 134, is then printed by outer nozzle 137；
(4) control system 3 controls servo motor 126 and opens, and realizes and rotates forward, and drives the rotation of driving gear 125, passes through master
Moving gear 125 is engaged with rack gear 122, pulls the movement of litter 123, and then print unit 13 is driven to move along a straight line, complete
At the printing of first row cell；
(5) control system 3 drives the position of the mobile cell factory of saddle 24, and then control system 3 controls servo electricity
Machine 126 inverts, and print unit 13 is driven to move along a straight line, and completes the printing of secondary series cell；
(6) repetitive cycling step (4) and step (5) are until complete the printing of last column cell.
The distance of a cell factory is the diameter of a cell in the step (5).
Bio-ink in the step (1) is by including that the component of following parts by weight is made: 2 parts of sodium alginate, shell is poly-
2.5 parts, 2 parts of acetic acid, 65 parts of water of sugar.
Embodiment 3
A kind of Biotype artificial liver cell 3D printing system, including printing mechanism 1, stamp pad 2, control system 3, it is described
Control system 3 be connected respectively with stamp pad 2 with the printing mechanism 1 by route；
The printing mechanism 1 includes mobile unit 12, print unit 13, support 11, and the mobile unit 12 is fixed
In on the support 11, the print unit 13 is connected with the mobile unit 12, the mobile unit 12, printing
Unit 13 is connected by route with the control system 3 respectively；
The mobile unit 12 includes the first supporting element 121, rack gear 122, litter 123, the second supporting element 124, active
Gear 125, servo motor 126, fixing sleeve 127, mobile box 128, the mobile box 128 are fixed on the support 11
On, first supporting element 121 is fixed in the mobile box 128, and the middle part of first supporting element 121 is equipped with
The shifting chute 1211 of perforation, one end of the rack gear 122 are placed in the shifting chute 1211, the other end and the litter
123 are connected, and second supporting element 124 is connected with the mobile box 128, and the litter 123 is across described the
Two supporting elements 124 are connected with the fixing sleeve 127, and the fixing sleeve 127 is placed on the outside of the print unit 13, institute
The driving gear 125 stated is meshed with the rack gear 122, and is connected with the servo motor 126, the servo motor
126 are connected by route with the control system 3.
The print unit 13 includes outer sleeve 131, inner sleeve 132, check valve 133, inner nozzle 134, fixed disk
135, fixing seat 136, outer nozzle 137, the outer sleeve 131 are fixed in the fixing sleeve 127, the inner sleeve
132 are fixed in the outer sleeve 131, and one end of the check valve 133 is connected with the inner sleeve 132, the other end
It is connected with the fixed disk 135 is passed through with the inner nozzle 134, the check valve 133 passes through route and the control
System 3 processed is connected, and the fixing seat 136 is located at the lower section of the inner nozzle 134, one end of the outer nozzle 137 and
The inner nozzle 134 is connected, and the other end passes through the fixing seat 136 and is located at outside the outer sleeve 131.
The stamp pad 2 includes table top 21, cell container 22, support frame 23, saddle 24, sliding rail 25, the branch
One end of support 23 is connected with the saddle 24, and the other end is connected with the table top 21, and the cell container 22 is solid
Due on the table top 21, the saddle 24 is matched with the sliding rail 25, and the controller passes through route and institute
The saddle 24 stated is connected.
The cell container 22 is rectangular configuration.
The sliding rail 25 is mutually perpendicular to the rack gear 122.
Using the Biotype artificial liver Method of printing of cell 3D printing system, include the following steps:
(1) bio-ink is configured, cell will wherein be added, preparation obtains bio-ink liquid containing cell；
(2) bat nozzle is placed in the surface of 22 1 interior angles of cell container；
(3) by the bio-ink liquid injection inner sleeve 132, control system 3 controls check valve 133 and opens, raw
Object ink liquid flows into inner nozzle 134, is then printed by outer nozzle 137；
(4) control system 3 controls servo motor 126 and opens, and realizes and rotates forward, and drives the rotation of driving gear 125, passes through master
Moving gear 125 is engaged with rack gear 122, pulls the movement of litter 123, and then print unit 13 is driven to move along a straight line, complete
At the printing of first row cell；
(5) control system 3 drives the position of the mobile cell factory of saddle 24, and then control system 3 controls servo electricity
Machine 126 inverts, and print unit 13 is driven to move along a straight line, and completes the printing of secondary series cell；
(6) repetitive cycling step (4) and step (5) are until complete the printing of last column cell.
The distance of a cell factory is 1.2 times of the diameter of a cell in the step (5).
Bio-ink in the step (1) is by including that the component of following parts by weight is made: 1 part of sodium alginate, shell is poly-
3 parts, 5 parts of acetic acid, 70 parts of water of sugar.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of Biotype artificial liver cell 3D printing system, it is characterised in that: including printing mechanism, stamp pad, control system
System, the control system pass through route respectively and are connected with the printing mechanism with stamp pad；
The printing mechanism includes mobile unit, print unit, support, and the mobile unit is fixed on the support
On, the print unit is connected with the mobile unit, and the mobile unit, print unit pass through route and institute respectively
The control system stated is connected；
The mobile unit includes the first supporting element, rack gear, litter, the second supporting element, driving gear, servo motor, fixation
Set, mobile box, the mobile box are fixed on the support, and first supporting element is fixed on the movement
In cabinet, the middle part of first supporting element is equipped with the shifting chute of perforation, and one end of the rack gear is placed in the movement
In slot, the other end is connected with the litter, and second supporting element is connected with the mobile box, and the litter is worn
It crosses second supporting element to be connected with the fixing sleeve, the fixing sleeve is placed on the outside of the print unit, institute
The driving gear stated is meshed with the rack gear, and is connected with the servo motor, and the servo motor passes through route
It is connected with the control system.
2. Biotype artificial liver cell 3D printing system according to claim 1, it is characterised in that: the printing list
Member includes outer sleeve, inner sleeve, check valve, inner nozzle, fixed disk, fixing seat, outer nozzle, and the outer sleeve is fixed on described
Fixing sleeve in, the inner sleeve is fixed in the outer sleeve, one end of the check valve and the inner sleeve
Be connected, the other end is connected with the fixed disk is passed through with the inner nozzle, the check valve pass through route with it is described
Control system is connected, the fixed seating in the lower section of the inner nozzle, one end of the outer nozzle with it is described interior
Nozzle is connected, and the other end passes through the fixed seating in outside the outer sleeve.
3. Biotype artificial liver cell 3D printing system according to claim 1, it is characterised in that: the stamp pad
Including table top, cell container, support frame, saddle, sliding rail, one end of the support frame is connected with the saddle, another
End is connected with the table top, and the cell container is fixed on the table top, the saddle and the sliding rail
It matches, the controller is connected by route with the saddle.
4. Biotype artificial liver cell 3D printing system according to claim 3, it is characterised in that: the cell holds
Device of receiving is rectangular configuration.
5. Biotype artificial liver cell 3D printing system according to claim 3, it is characterised in that: the sliding rail with
The rack gear is mutually perpendicular to.
6. using the Method of printing of the Biotype artificial liver cell 3D printing system of any of claims 1-5,
It is characterized in that: including the following steps:
(1) bio-ink is configured, cell will wherein be added, preparation obtains bio-ink liquid containing cell；
(2) bat nozzle is placed in the surface of one interior angle of cell container；
(3) by the bio-ink liquid injection inner sleeve, control system controls check valve and opens, bio-ink liquid flow
Enter in inner nozzle, is then printed by outer nozzle；
(4) control system control servo motor is opened, and is realized and is rotated forward, drives the rotation of driving gear, pass through driving gear and tooth
The engagement of item pulls the movement of litter, and then print unit is driven to move along a straight line, and completes the printing of first row cell；
(5) position of the mobile cell factory of control system driving saddle, then control system control servo motor reversion, band
Dynamic print unit moves along a straight line, and completes the printing of secondary series cell；
(6) repetitive cycling step (4) and step (5) are until complete the printing of last column cell.
7. Method of printing according to claim 6, it is characterised in that: in the step (5) cell factory away from
From the diameter for being more than or equal to a cell.
8. Method of printing according to claim 6, it is characterised in that: the bio-ink in the step (1) is by including
The component of following parts by weight is made:
9. bio-ink according to claim 6, it is characterised in that: the hydrophilic agent is one in chitosan or gelatin
Kind.
10. bio-ink according to claim 6, it is characterised in that: the chaotropic agent is acetic acid, hydrochloric acid, nitrous acid
Or one of phosphoric acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910517512.8A CN110229740A (en) | 2019-06-14 | 2019-06-14 | A kind of Biotype artificial liver cell 3D printing system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910517512.8A CN110229740A (en) | 2019-06-14 | 2019-06-14 | A kind of Biotype artificial liver cell 3D printing system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110229740A true CN110229740A (en) | 2019-09-13 |
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| US20150035206A1 (en) * | 2013-08-01 | 2015-02-05 | Sartorius Stedim Biotech Gmbh | Single-use biological 3 dimensional printer |
| CN106399078A (en) * | 2015-07-29 | 2017-02-15 | 清华大学 | Heterogeneous cell three-dimensional printing system and heterogeneous cell three-dimensional printing method |
| CN107245430A (en) * | 2017-06-23 | 2017-10-13 | 广州迈普再生医学科技有限公司 | A kind of cell 3D printing system and Method of printing |
| CN109806441A (en) * | 2019-01-25 | 2019-05-28 | 王靖童 | A kind of artificial heart printing bionical construction method of 3D printing equipment and artificial heart |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150035206A1 (en) * | 2013-08-01 | 2015-02-05 | Sartorius Stedim Biotech Gmbh | Single-use biological 3 dimensional printer |
| CN106399078A (en) * | 2015-07-29 | 2017-02-15 | 清华大学 | Heterogeneous cell three-dimensional printing system and heterogeneous cell three-dimensional printing method |
| CN107245430A (en) * | 2017-06-23 | 2017-10-13 | 广州迈普再生医学科技有限公司 | A kind of cell 3D printing system and Method of printing |
| CN109806441A (en) * | 2019-01-25 | 2019-05-28 | 王靖童 | A kind of artificial heart printing bionical construction method of 3D printing equipment and artificial heart |
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