CN109805375A - A kind of functional food and preparation method thereof improving organum visuale's oxidation resistance - Google Patents
A kind of functional food and preparation method thereof improving organum visuale's oxidation resistance Download PDFInfo
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- CN109805375A CN109805375A CN201910080859.0A CN201910080859A CN109805375A CN 109805375 A CN109805375 A CN 109805375A CN 201910080859 A CN201910080859 A CN 201910080859A CN 109805375 A CN109805375 A CN 109805375A
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Abstract
The invention belongs to field of food, specifically disclose a kind of functional food and preparation method thereof for improving organum visuale's oxidation resistance.The functional food for improving organum visuale's oxidation resistance contains 10~50 parts by weight of fucoxanthin-albumen composition, 5~30 parts by weight of polyphenol extract, 1~5 parts by weight of Anoectochilus, 1~5 parts by weight of abalone shell extract, 0.1~1 parts by weight of zinc gluconate, degradation 0.1~1 parts by weight of sulfated polysaccharide, remaining is water and/or solid filler auxiliary material.Contain the ingredients such as fucoxanthin-albumen composition, polyphenol extract, Anoectochilus, abalone shell extract, zinc gluconate, degradation sulfated polysaccharide in the functional food provided by the invention for improving organum visuale's oxidation resistance; fucoxanthin metabolin (fucoxanthol) can be promoted to transport in the targeting of organum visuale; and the above component is proportionally subjected to prescription design; synergistic protective effect can be played to organum visuale, to significantly improve the anti-oxidation stress ability of organum visuale.
Description
Technical field
The invention belongs to field of food, specifically disclose a kind of functional food and its system for improving organum visuale's oxidation resistance
Preparation Method.
Background technique
Eyes are human physiological metabolism's organs the most active, due to the needs that vision generates, eyes are caused (especially to regard
Nethike embrane) it is in the environment of high oxygen pressure, high polyunsaturated fatty acid, Gao Juguang for a long time, it is highly prone to oxidativestress damage.With this
Simultaneously as the needs of the modern life and working method, the video frequency terminal apparatus such as wideband mobile phone, computer, TV are very general
And it is exposed to light radiation environment for a long time and has been further exacerbated by the stress damage for using asthenopia and organum visuale.As children, blueness are few
The disease incidence of the ophthalmology diseases such as the rate of myopia and cataract, treating senile maculopathy in year is constantly soaring, depending on health problem
As public's focus of attention.Therefore, the angle supplemented from diet nutritional, improves the oxidation resistance of organum visuale, is to answer
To the effective means for working as forward sight health problem.
Fucoxanthin is the secondary metabolite that brown alga adapts to the extreme luminous environment in ocean, and unique allene structure imparts
Its high anti-oxidation activity (13.5 times of vitamin E), and have proven to have the function of good inhibition retinal light damage (Liu et
al.,2016).For example, patent application " new application that fucoxanthin is used for sight protectio " (CN105878230A), a kind of " protect liver
The functional food of improving eyesight " (CN105996027A) discloses the eyesight protection function food using fucoxanthin as function factor.
However, fucoxanthin has that absorptivity is low, stability is poor, the obvious drop for resulting in its sight protectio effect is directly taken
It is low.Currently, targeting transhipment by the organum visuale that albumen self assembly improves fucoxanthin and its in eyesight protection function food
Application not yet appear in the newspapers.
Summary of the invention
In view of the deficiencies of the prior art, it is an object of the present invention to design by product formula, a kind of composition is provided
Reasonable composition, safety is without side-effects, can effectively improve the functional food of organum visuale's oxidation resistance.
Another mesh of the invention is to provide a kind of production work that can be improved fucoxanthin organum visuale and target conevying efficiency
Skill, to meet requirement of the exploitation eyesight protection function food to low cost, suitable scale industrial production.
Specifically, the present invention provides a kind of functional food for improving organum visuale's oxidation resistance, wherein the raising view
The functional food of organ oxidation resistance contains 10~50 parts by weight of fucoxanthin-albumen composition, 5~30 weight of polyphenol extract
Measure part, 1~5 parts by weight of Anoectochilus, 1~5 parts by weight of abalone shell extract, 0.1~1 parts by weight of zinc gluconate, drop
0.1~1 parts by weight of sulfated polysaccharide are solved, remaining is water and/or solid filler auxiliary material.
Preferably, in the fucoxanthin-albumen composition mass ratio of fucoxanthin and carrier protein be 1:(8~
12)。
Preferably, carrier protein contained in the fucoxanthin-albumen composition is selected from bovine serum albumin(BSA), whey egg
At least one of white, zeins, casein, soybean protein and oyster peptide.
Preferably, the raw material of the polyphenol extract is selected from least one of longan, tealeaves, cassia seed and peanut coat.
Preferably, in the Anoectochilus alkaloid content >=2wt%.
Preferably, the degradation sulfated polysaccharide origin derived from seaweed or marine animal sulfated polysaccharide through biology, physics or
What chemical method hydrolyzed, the molecular weight≤6000Da and sulfate group content >=20wt% of the degradation sulfated polysaccharide.
Preferably, the functional food for improving organum visuale's oxidation resistance is with capsule, tablet, electuary, oral solution or drink
The form of material uses.
Term " solid filler auxiliary material " includes common some additives and shaping additive in food.In addition, water and/
Or the dosage of solid filler auxiliary material should depend on the circumstances, by the fucoxanthin-albumen composition, polyphenol extract, gold
Line lotus extract, abalone extract, zinc gluconate and degradation sulfated polysaccharide be configured to capsule, tablet, electuary, oral solution or
Subject to the form use of beverage, this those skilled in the art can be known, therefore not to repeat here.
The present invention also provides the preparation methods of the functional food for improving organum visuale's oxidation resistance, wherein the party
Method the following steps are included:
(1) fucoxanthin-albumen composition preparation: compound concentration is the carrier protein aqueous solution of 0.1~1wt%, and will
Its pH value is adjusted to 10~12, and fatty acid is then added and stirs evenly simultaneously ultrasonic disperse, obtains carrier protein solution；Rock algae is yellow
Plain powder and the carrier protein solution are stirred to react 10~60 minutes, and the pH value that gained is stirred to react product is recalled to 6
~8, fucoxanthin-albumen composition is obtained after spray drying；
(2) by the fucoxanthin-albumen composition, polyphenol extract, Anoectochilus, abalone shell extract, Portugal
Grape saccharic acid zinc, degradation sulfated polysaccharide, water and/or solid-filling agent auxiliary material mix in proportion, later that gained liquid mixture is equal
Matter, filtering, or obtained solid mixture is sieved, it is improved the functional food of organum visuale's oxidation resistance.
Preferably, in step (1), the weight ratio of the fatty acid and the carrier protein solution in terms of carrier protein is
1:(18~22).
Preferably, in step (1), the weight ratio of the fucoxanthin and the carrier protein solution in terms of carrier protein
For 1:(8~12).Wherein, the fucoxanthin powder and the carrier protein solution being stirred to react can be by the rock algae
Flavine uses a small amount of solvatrope, is added in the carrier protein solution later, is stirred to react 10~60 minutes, preferably stirring is anti-
It answers 20~40 minutes.
Preferably, in step (1), the fatty acid is selected from oleic acid, linoleic acid, palmitinic acid, arachidonic acid and 22
At least one of carbon acid.
Preferably, in step (1), it is tart flavour that the pH value for being stirred to react product, which is recalled to reagent used by 6~8,
Agent, the acid are selected from least one of acetic acid, citric acid, lactic acid, tartaric acid, malic acid and glucolactone.
Preferably, the polyphenol extract is prepared in accordance with the following methods: by longan, tealeaves, cassia seed and peanut coat
At least one of raw material after drying, crushing, sieving, 1:(10~20 by volume) alcohol hydrochloric acid aqueous solution is added, in room
Temperature is lower to be extracted 1~5 hour, and centrifuging and taking supernatant is concentrated under reduced pressure, purifying.Wherein, in the alcohol hydrochloric acid aqueous solution ethyl alcohol body
Product concentration is 60~80vt% and the volumetric concentration of hydrochloric acid is 0.05~0.2vt%.The mode of the purifying preferably will decompression
Enriched product successively carries out macroporous resin adsorption, water elution, ethanol elution, reduced pressure and (vacuum or freezing) drying.
Preferably, the Anoectochilus is prepared in accordance with the following methods: fresh roxburgh anoectochilus terminal bud being cleaned, dry, powder
After broken, sieving, 1:(15~25 by volume) deionized water is added, boiling water is cooled to room temperature after cooking 0.5~2 hour, is centrifuged
Supernatant is taken, is spray-dried.
Preferably, the abalone shell extract is prepared in accordance with the following methods: abalone shells being cleaned, is dry, are crushed, mistake
After sieve, 1:(5~15 by volume) ethanol water that volumetric concentration is 40~60vt% is added, room temperature is extracted 1~5 hour
Afterwards, it is centrifuged, takes supernatant, be concentrated under reduced pressure, spray drying.
The present invention makes full use of " multipath, multiple target point, synergistic effect " thought in Traditional Chinese medical theory, will have difference
The polyphenol extract of function and action pathway, Anoectochilus, abalone shell extract, zinc gluconate and fucoxanthin-egg
The effect of white compound carries out scientific composition compatibility, realizes each ingredient is complementary, synergistic effect, thus the organum visuale that is significantly improved
The functional food of oxidation resistance.
Compared with prior art, the invention has the following advantages that
(1) contain fucoxanthin-albumen composition, polyphenol extract, Anoectochilus, abalone in the functional food
These components are proportionally carried out prescription design by the ingredients such as shell extract, zinc gluconate, degradation sulfated polysaccharide, can be right
Organum visuale plays synergistic protective effect, to significantly improve the anti-oxidation stress ability of organum visuale.
(2) fucoxanthin-albumen composition in the functional food solves that fucoxanthin absorption efficiency is low, stability
The problem of difference, the targeting for significantly improving fucoxanthin metabolite fucoxanthol in organum visuale is transported, to improve rock algae
The nutrition balance of flavine protection eyesight.
(3) raw materials used is food-borne, integration of drinking and medicinal herbs and the article that can be used for health food, and degradation sulfated polysaccharide has
Good bacteriostasis can effectively ensure that the safe and healthy of product, without adding any preservative, have safe and non-toxic, suitable
In the advantage of any crowd.
(4) modern food production technology, stable product quality, simple production process, low in cost, suitable scale are used
Industrial production.
Detailed description of the invention
Fig. 1 is the preparation technology flow chart of the functional food provided by the invention for improving organum visuale's oxidation resistance；
Fig. 2 is the fucoxanthol liquid phase detection figure in naive mice eyeball；
Fig. 3 is the fucoxanthol liquid phase detection figure in embodiment 1 (fucoxanthin-albumen composition group) eyeball of mouse；
Fig. 4 be blank group, in 1 formula group eyeball of mouse of embodiment total antioxidant capacity comparison diagram.
Specific embodiment
The embodiment of the present invention is described below in detail, the examples of the embodiments are intended to be used to explain the present invention, and cannot
It is interpreted as limitation of the present invention.In the examples where no specific technique or condition is specified, described according to the literature in the art
Technology or conditions or carried out according to product description.Reagents or instruments used without specified manufacturer is that can lead to
Cross the conventional products of commercially available acquisition.
Embodiment 1
The functional food of organum visuale's oxidation resistance, including following step are improved according to process flow preparation as shown in Figure 1
It is rapid:
(1) fucoxanthin-albumen composition preparation: compound concentration is the Bovine Serum Albumin in Aqueous Solution of 0.5wt%, is used in combination
Sodium hydroxide adjusts pH value to 11, and oleic acid (mass ratio of oleic acid and bovine serum albumin(BSA) is 1:18) is then added and stirs evenly,
And ultrasound 30 minutes, obtain carrier protein solution；Fucoxanthin powder (mass ratio of fucoxanthin and bovine serum albumin(BSA) is 1:
8) it with after a small amount of alkaline ethanol hydrotropy, is added in carrier protein solution, is reacted 30 minutes under stirring condition, will be mixed with acetic acid
The pH value of solution is recalled to 7.0 (self assemblies), and spray drying obtains fucoxanthin-bovine serum albumin(BSA) compound.
(2) (in accordance with the following methods with fucoxanthin -10 parts by weight of bovine serum albumin(BSA) compound, longan polyphenol extract
It is prepared: after being dried by remaining shell after dry longan demucilage and fruit stone, crush, be sieved, 1:15 by volume
Alcohol hydrochloric acid aqueous solution is added, and (volumetric concentration of ethyl alcohol is 70vt% in alcohol hydrochloric acid aqueous solution and the volumetric concentration of hydrochloric acid is
0.1vt%), it extracts 2 hours at room temperature, centrifuging and taking supernatant, is concentrated under reduced pressure, is washed through macroporous resin adsorption, water elution, ethyl alcohol
Longan polyphenol extract is obtained after de-, reduced pressure, vacuum or freeze-drying) 30 parts by weight, Anoectochilus (wherein biology
The content of alkali is 2.7wt%, is prepared in accordance with the following methods: after fresh roxburgh anoectochilus terminal bud is cleaned up, is dried, is crushed, is sieved,
Deionized water is added in 1:20 by volume, and boiling water is cooled to room temperature after cooking 2 hours, centrifuging and taking supernatant, spray-dried
To Anoectochilus) 1 parts by weight, abalone shell extract (be prepared in accordance with the following methods: abalone shells being cleaned, is dry, powder
After broken, sieving, the ethanol water that volumetric concentration is 50vt% is added in 1:10 by volume, after room temperature is extracted 2 hours, centrifugation,
Take supernatant, be concentrated under reduced pressure, be spray-dried after obtain abalone shell extract) 1 parts by weight, 0.1 parts by weight of zinc gluconate, drop
Solving kelp polysaccharide sulfate, (molecular weight is≤6000Da, and sulfate group content weighs above-mentioned for the ratio of 0.1 parts by weight of 32wt%)
Appropriate magnesium stearate and starch is added in prescription in each component, mixes, is packed into ebonite with Autocapsulefillingmachine after sieving
Capsule, every loading amount 0.5g, sterilizing are packed to get capsule class functional food.
Embodiment 2
The functional food of organum visuale's oxidation resistance, including following step are improved according to process flow preparation as shown in Figure 1
It is rapid:
(1) fucoxanthin-albumen composition preparation: compound concentration is the aqueous whey protein solution of 0.5wt%, and uses hydrogen-oxygen
Change sodium and adjust pH value to 11, linoleic acid (mass ratio of linoleic acid and lactalbumin is 1:20) is then added and stirs evenly, and surpasses
Sound 30 minutes, obtain carrier protein solution；Fucoxanthin powder (mass ratio of fucoxanthin and lactalbumin is 1:10) is with less
It after measuring alkaline ethanol hydrotropy, is added in carrier protein solution, is reacted 30 minutes under stirring condition, with citric acid by mixed solution
PH value recall to 7.0 (self assemblies), spray drying obtains fucoxanthin-lactalbumin complex.
(2) it (is prepared in accordance with the following methods with 30 parts by weight of fucoxanthin-lactalbumin complex, tea leaf polyphenols extract
Obtain: after fresh tea leaf is dry, crushing, sieving, alcohol hydrochloric acid aqueous solution (alcohol hydrochloric acid aqueous solution is added in 1:15 by volume
The volumetric concentration of middle ethyl alcohol is 70vt% and the volumetric concentration of hydrochloric acid is 0.1vt%), it extracts 2 hours at room temperature, in centrifuging and taking
Clear liquid is concentrated under reduced pressure, and obtains tea after macroporous resin adsorption, water elution, ethanol elution, reduced pressure, vacuum or freeze-drying
Leaf polyphenol extract) 17.5 parts by weight, Anoectochilus (wherein the content of alkaloid be 2.4wt%, make in accordance with the following methods
Standby to obtain: after fresh roxburgh anoectochilus terminal bud is cleaned up, is dried, is crushed, is sieved, deionized water, boiling water boiling is added in 1:15 by volume
System is cooled to room temperature after 2 hours, and centrifuging and taking supernatant is spray-dried to obtain Anoectochilus) 3 parts by weight, abalone shells mention
Object is taken (to be prepared in accordance with the following methods: abalone shells being cleaned, is dry, are crushed, after sieving, it is dense that volume is added in 1:5 by volume
Degree is the ethanol water of 40vt%, and after room temperature is extracted 5 hours, centrifugation takes supernatant, after being concentrated under reduced pressure, being spray-dried
To abalone shell extract) 3 parts by weight, 0.55 parts by weight of zinc gluconate, degradation Eucheuma sulfated polysaccharide (molecular weight be≤
6000Da, sulfate group content weigh above-mentioned each component for the ratio of 0.55 parts by weight of 26wt%), suitable paste are then added
One or more of essence, starch, microcrystalline cellulose and lactose auxiliary material is uniformly mixed, with appropriate 70% (v/v) ethanol wet,
Softwood processed crosses the granulation of 30 meshes, and in 70-80 DEG C of drying, with 60 mesh sieves, tabletting obtains tablet, every 0.2g, sterilizing, packet
Dress is to get tablet class functional food.
Embodiment 3
The functional food of organum visuale's oxidation resistance, including following step are improved according to process flow preparation as shown in Figure 1
It is rapid:
(1) fucoxanthin-albumen composition preparation: compound concentration is the corn alcohol soup-dissolving protein solution of 0.5wt%, is used in combination
Sodium hydroxide adjusts pH value to 11, and it is equal that palmitinic acid (mass ratio of palmitinic acid and zeins is 1:22) stirring is then added
It is even, and ultrasound 30 minutes, obtain carrier protein solution；Fucoxanthin powder (the mass ratio of fucoxanthin and zeins
It after a small amount of alkaline ethanol hydrotropy of 1:12), is added in carrier protein solution, is reacted 30 minutes under stirring condition, use grape
Saccharic acid lactone recalls to the pH value of mixed solution to 7.0 (self assemblies), and spray drying obtains fucoxanthin-zeins
Compound.
(2) with fucoxanthin -50 parts by weight of zeins compound, cassia seed polyphenol extract (according to lower section
Method is prepared: after cassia seed is dry, crushing, sieving, alcohol hydrochloric acid aqueous solution (ethylate sour water is added in 1:15 by volume
The volumetric concentration of ethyl alcohol is 70vt% in solution and the volumetric concentration of hydrochloric acid is 0.1vt%), it extracts 2 hours at room temperature, centrifugation
Supernatant is taken, is concentrated under reduced pressure, after macroporous resin adsorption, water elution, ethanol elution, reduced pressure, vacuum or freeze-drying
To cassia seed polyphenol extract) 5 parts by weight, Anoectochilus (wherein the content of alkaloid be 2.0wt%, according to lower section
Method is prepared: after fresh roxburgh anoectochilus terminal bud is cleaned up, is dried, is crushed, is sieved, deionized water, boiling is added in 1:25 by volume
Boiling system is cooled to room temperature after 1 hour, centrifuging and taking supernatant, spray-dried to obtain Anoectochilus) 5 parts by weight, abalone
Shell extract (is prepared in accordance with the following methods: abalone shells being cleaned, is dry, are crushed, after sieving, 1:15 is added by volume
Volumetric concentration is the ethanol water of 60vt%, and after room temperature is extracted 1 hour, centrifugation takes supernatant, is concentrated under reduced pressure, done by spraying
Abalone shell extract is obtained after dry) 5 parts by weight, 1 parts by weight of zinc gluconate, degradation sea cucumber sulfated polysaccharide (molecular weight be≤
6000Da, sulfate group content weigh above-mentioned each component for the ratio of 1 parts by weight of 20wt%), and appropriate soluble shallow lake is then added
Powder, dextrin, sucrose, lactose and mannitol are one of or several, and with appropriate 70% (v/v) ethanol wet, softwood processed crosses 20 mesh
Sieve granulation, 70-80 DEG C of drying, 60 mesh whole grains, sterilizing are packed to get electuary class functional food.
Embodiment 4
The functional food of organum visuale's oxidation resistance, including following step are improved according to process flow preparation as shown in Figure 1
It is rapid:
(1) fucoxanthin-albumen composition preparation: compound concentration is the oyster peptide aqueous solution of 0.5wt%, and uses hydroxide
Sodium adjusts pH value to 11, and docosahexaenoic acid (mass ratio of docosahexaenoic acid and oyster peptide is 1:20) is then added and stirs
It mixes uniformly, and ultrasound 30 minutes, obtains carrier protein solution；(mass ratio of fucoxanthin and oyster peptide is fucoxanthin powder
It 1:10) with after a small amount of alkaline ethanol hydrotropy, is added in carrier protein solution, is reacted 30 minutes under stirring condition, use malic acid
The pH value of mixed solution is recalled to 7.0 (self assemblies), spray drying obtains fucoxanthin-oyster peptide complexes.
(2) it (is prepared in accordance with the following methods with fucoxanthin -10 parts by weight of oyster peptide complexes, peanut coat polyphenol extract
Obtain: after peanut coat is dry, crushing, sieving, alcohol hydrochloric acid aqueous solution is added (in alcohol hydrochloric acid aqueous solution in 1:15 by volume
The volumetric concentration of ethyl alcohol is 70vt%, and the volumetric concentration of hydrochloric acid is 0.1vt%), it extracts 2 hours at room temperature, centrifuging and taking supernatant
Liquid is concentrated under reduced pressure, and obtains peanut after macroporous resin adsorption, water elution, ethanol elution, reduced pressure, vacuum or freeze-drying
Clothing polyphenol extract) 5 parts by weight, Anoectochilus (wherein the content of alkaloid be 2.3wt%, prepare in accordance with the following methods
Obtain: after fresh roxburgh anoectochilus terminal bud is cleaned up, is dried, is crushed, is sieved, deionized water is added in 1:20 by volume, and boiling water cooks
It is cooled to room temperature after 1.5 hours, centrifuging and taking supernatant is spray-dried to obtain Anoectochilus) 1.5 parts by weight, abalone shells
Extract (is prepared in accordance with the following methods: abalone shells being cleaned, is dry, are crushed, after sieving, body is added in 1:10 by volume
The ethanol water that product concentration is 50vt%, after room temperature is extracted 2 hours, centrifugation takes supernatant, is concentrated under reduced pressure, is spray-dried
After obtain abalone shell extract) 1.5 parts by weight, 0.25 parts by weight of zinc gluconate, degradation fish-bone sulfated polysaccharide (molecular weight be≤
6000Da, sulfate group content weigh above-mentioned each component for the ratio of 0.75 parts by weight of 26wt%), are then dissolved in water, are added
Suitable sweetener and stabilizer mix, homogeneous, filter, and sterilization is packed to get beverage class functional food.
Comparative example 1
The functional food for improving oxidation resistance is prepared according to the method for embodiment 1, unlike, it does not include step (1)
And substitute the fucoxanthin in step (2)-bovine serum albumin(BSA) compound using the fucoxanthin of identical weight part, joined
Than capsule class functional food.
Comparative example 2
The functional food for improving oxidation resistance is prepared according to the method for embodiment 1, unlike, it will be in step (2)
Longan polyphenol extract is substituted using the degradation kelp polysaccharide sulfate of identical weight part, obtains reference capsule class functional food.
Comparative example 3
The functional food for improving oxidation resistance is prepared according to the method for embodiment 1, unlike, it will be in step (2)
Anoectochilus is substituted using the degradation kelp polysaccharide sulfate of identical weight part, obtains reference capsule class functional food.
Comparative example 4
The functional food for improving oxidation resistance is prepared according to the method for embodiment 1, unlike, it will be in step (2)
Abalone extract is substituted using the degradation kelp polysaccharide sulfate of identical weight part, obtains reference capsule class functional food.
Functional evaluation test:
(1) evaluation is transported in targeting of the fucoxanthin in organum visuale
Given the test agent: fucoxanthin obtained in the embodiment of the present invention 1-bovine serum albumin(BSA) compound.
Sample treatment: 1:10 is dissolved in bovine serum albumin(BSA) compound 1 gained fucoxanthin of Example-by volume
In ionized water, as test liquid.
Experimental animal: SPF grades of Female ICR mices, 15-20g are purchased from Shanghai Slac Experimental Animal Co., Ltd..
Mouse basal feed ingredient: carbohydrate 64%, protein 21%, fat 4%, fiber 5%, moisture 6%.Above-mentioned feed is equal
It is purchased from Shanghai Slac Experimental Animal Co., Ltd..Raising room temperature: 23 ± 2 DEG C, relative humidity 55 ± 5%.
Animal packet and processing method: after mouse adaptable fed 1 week, being randomly divided into 2 groups according to weight, and every group 10,
Respectively blank group (stomach-filling deionized water), processing group (stomach-filling test liquid).The each 200 μ L test liquid of stomach-filling of every mouse is gone
Ionized water after continuous gavage 2 weeks, is won eyeball of mouse, is ground after liquid nitrogen frozen 1 time a day, and is 1:1's with volume ratio
Ethyl acetate-light petrol mixed solution extracts 3 times, and combined extract takes supernatant after centrifugation, is concentrated into 50 μ L with the nitrogen method of blowing,
With the changes of contents of fucoxanthol in high performance liquid chromatography (HPLC) analysis concentrate.
Experimental result:
The chromatogram of HPLC analysis of control group and the fucoxanthol in processing group eyeball of mouse is shown in Fig. 2 and Fig. 3 respectively.From figure
2 and Fig. 3, which can be seen that, obtains the quantitative analysis regression equation of fucoxanthol according to fucoxanthol concentration and its HPLC peak area,
The peak area of combination processing group, obtaining the fucoxanthol concentration in concentrate is 258.7pmol/mL.Illustrate with fucoxanthin-egg
White compound be eyesight protection function food core function ingredient can effectively realize fucoxanthol in the targeted delivery of organum visuale,
To improve the protective effect to eyesight.
(2) organum visuale's total antioxidant capacity is evaluated
Given the test agent: formulation product obtained in 1- of embodiment of the present invention embodiment 4 and comparative example 1- comparative example 4.
Sample treatment: by formulation product, 1:10 is directly dissolved in deionized water by volume, as test liquid.
Experimental animal: SPF grades of Female ICR mices, 15-20g are purchased from Shanghai Slac Experimental Animal Co., Ltd..
Mouse basal feed ingredient: carbohydrate 64%, protein 21%, fat 4%, fiber 5%, moisture 6%.Above-mentioned feed is equal
It is purchased from Shanghai Slac Experimental Animal Co., Ltd..Raising room temperature: 23 ± 2 DEG C, relative humidity 55 ± 5%.
Animal packet and processing method: after mouse adaptable fed 1 week, being randomly divided into 9 groups according to weight, and every group 10,
Respectively blank group (stomach-filling deionized water) and processing group (stomach-filling test liquid).The each 200 μ L test liquid of stomach-filling of every mouse or
Deionized water after continuous gavage 2 weeks, wins eyeball of mouse 1 time a day, with the anti-oxidant anti-oxidant energy of kit assay organum visuale
The variation of power.
Experimental result:
The result shows that by continuous stomach-filling in 2 weeks, 4 products therefrom of embodiment 1- embodiment 4 and comparative example 1- comparative example
Total antioxidant capacity has been respectively increased 39.3% ± 3.6%, 37.2% ± 2.4%, 41.1% ± 1.8%, 39.8% ±
2.7%, 13.8% ± 1.2%, 21.5% ± 4.3%, 23.6% ± 3.7%, 28.4% ± 2.1%.Wherein, blank group and reality
It applies eyeball of mouse total antioxidant capacity result in 1 products obtained therefrom group of example and sees Fig. 4.From fig. 4, it can be seen that by continuous stomach-filling in 2 weeks,
The total antioxidant capacity of mouse eye is 9.42U/mL in blank group, the total antioxidation of mouse eye in 1 products obtained therefrom of embodiment
Ability is 13.12U/mL, improves 39.3% ± 3.6% than blank group.It is possible thereby to illustrate, the eyesight that the present invention is improved is protected
Protective function food formula can effectively improve the anti-oxidation stress ability of organum visuale, can effectively reduce myopia and occur and reduce retina
The occurrence risk of disease.
The preferred embodiment of the present invention has been described above in detail, still, during present invention is not limited to the embodiments described above
Detail within the scope of the technical concept of the present invention can be with various simple variants of the technical solution of the present invention are made, this
A little simple variants all belong to the scope of protection of the present invention.
It is further to note that specific technical features described in the above specific embodiments, in not lance
In the case where shield, it can be combined in any appropriate way.In order to avoid unnecessary repetition, the present invention to it is various can
No further explanation will be given for the combination of energy.
In addition, various embodiments of the present invention can be combined randomly, as long as it is without prejudice to originally
The thought of invention, it should also be regarded as the disclosure of the present invention.
Claims (10)
1. a kind of functional food for improving organum visuale's oxidation resistance, which is characterized in that raising organum visuale's oxidation resistance
Functional food contain 10~50 parts by weight of fucoxanthin-albumen composition, 5~30 parts by weight of polyphenol extract, roxburgh anoectochilus terminal bud and mention
Take 1~5 parts by weight of object, 1~5 parts by weight of abalone shell extract, 0.1~1 parts by weight of zinc gluconate, degradation sulfated polysaccharide 0.1
~1 parts by weight, remaining is water and/or solid filler auxiliary material.
2. the functional food according to claim 1 for improving organum visuale's oxidation resistance, which is characterized in that the rock algae is yellow
The mass ratio of fucoxanthin and carrier protein is 1:(8~12 in element-albumen composition).
3. the functional food according to claim 1 for improving organum visuale's oxidation resistance, which is characterized in that the rock algae is yellow
Carrier protein contained in element-albumen composition is selected from bovine serum albumin(BSA), lactalbumin, zeins, casein, big
At least one of legumin and oyster peptide.
4. improving the functional food of organum visuale's oxidation resistance described in any one of -3 according to claim 1, feature exists
In the raw material of the polyphenol extract is selected from least one of longan, tealeaves, cassia seed and peanut coat.
5. improving the functional food of organum visuale's oxidation resistance described in any one of -3 according to claim 1, feature exists
In content >=2wt% of alkaloid in the Anoectochilus.
6. improving the functional food of organum visuale's oxidation resistance described in any one of -3 according to claim 1, feature exists
In the degradation sulfated polysaccharide origin is derived from the sulfated polysaccharide of seaweed or marine animal through biological, physically or chemically method hydrolyzes
It arrives, the molecular weight≤6000Da and sulfate group content >=20wt% of the degradation sulfated polysaccharide.
7. improving the functional food of organum visuale's oxidation resistance described in any one of -3 according to claim 1, feature exists
In the functional food for improving organum visuale's oxidation resistance is used in the form of capsule, tablet, electuary, oral solution or beverage.
8. the preparation method of the functional food of organum visuale's oxidation resistance is improved described in any one of claim 1-7,
It is characterized in that, method includes the following steps:
(1) fucoxanthin-albumen composition preparation: compound concentration is the carrier protein aqueous solution of 0.1~1wt%, and by its pH
Value is adjusted to 10~12, and fatty acid is then added and stirs evenly simultaneously ultrasonic disperse, obtains carrier protein solution；By fucoxanthin powder
It is last to be stirred to react 10~60 minutes with the carrier protein solution, and the pH value that gained is stirred to react product is recalled to 6~8,
Fucoxanthin-albumen composition is obtained after spray drying；
(2) by the fucoxanthin-albumen composition, polyphenol extract, Anoectochilus, abalone shell extract, glucose
Sour zinc, degradation sulfated polysaccharide, water and/or solid-filling agent auxiliary material mix in proportion, later by gained liquid mixture homogeneous, mistake
Filter, or obtained solid mixture is sieved, it is improved the functional food of organum visuale's oxidation resistance.
9. the preparation method of the functional food according to claim 8 for improving organum visuale's oxidation resistance, which is characterized in that
In step (1), the weight ratio of the fatty acid and the carrier protein solution in terms of carrier protein is 1:(18~22)；It is described
The weight ratio of fucoxanthin and the carrier protein solution in terms of carrier protein is 1:(8~12)；The fatty acid is selected from oil
At least one of acid, linoleic acid, palmitinic acid, arachidonic acid and docosahexaenoic acid；By the product of being stirred to react
It is acid that pH value, which is recalled to reagent used by 6~8, and the acid is selected from acetic acid, citric acid, lactic acid, tartaric acid, apple
At least one of acid and glucolactone.
10. the preparation method of the functional food according to claim 8 for improving organum visuale's oxidation resistance, feature exist
In the polyphenol extract is prepared in accordance with the following methods: by least one of longan, tealeaves, cassia seed and peanut coat
Raw material is after drying, crushing, sieving, 1:(10~20 by volume) alcohol hydrochloric acid aqueous solution is added, 1~5 is extracted at room temperature
Hour, centrifuging and taking supernatant is concentrated under reduced pressure, purifying；In the alcohol hydrochloric acid aqueous solution volumetric concentration of ethyl alcohol be 60~
The volumetric concentration of 80vt% and hydrochloric acid is 0.05~0.2vt%；Preferably, the mode of the purifying be will be concentrated under reduced pressure product according to
Secondary progress macroporous resin adsorption, water elution, ethanol elution, reduced pressure and drying；
The Anoectochilus is prepared in accordance with the following methods: fresh roxburgh anoectochilus terminal bud being cleaned, is dry, is crushed, after sieving, is pressed
Volume ratio 1:(15~25) deionized water is added, boiling water is cooled to room temperature after cooking 0.5~2 hour, and centrifuging and taking supernatant is spraying
It is dry；
The abalone shell extract is prepared in accordance with the following methods: abalone shells being cleaned, is dry, are crushed, after sieving, by volume
Than 1:(5~15) ethanol water that volumetric concentration is 40~60vt% is added, after room temperature is extracted 1~5 hour, centrifugation is taken
Clear liquid is concentrated under reduced pressure, spray drying.
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