CN109804751A - Accelerate the method, apparatus and gained Aged Seeds of Seed Aging - Google Patents
Accelerate the method, apparatus and gained Aged Seeds of Seed Aging Download PDFInfo
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- CN109804751A CN109804751A CN201910298989.1A CN201910298989A CN109804751A CN 109804751 A CN109804751 A CN 109804751A CN 201910298989 A CN201910298989 A CN 201910298989A CN 109804751 A CN109804751 A CN 109804751A
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Abstract
The present invention discloses a kind of method, apparatus and gained Aged Seeds for accelerating Seed Aging.The method of acceleration Seed Aging of the invention includes the steps that seed is made to be in confined space, wherein the volumetric concentration of oxygen is 90% or more in control confined space, humidity is 50%RH or less and temperature is 40-50 DEG C, and water content control is in 3%-10% before Seed Aging.The present invention can the directly quick aging under low water content state, on the one hand preferably simulate Seed storage condition aging, the seed after another aspect aging is almost the same in each trait expression and typical aging method of germination period.
Description
Technical field
The present invention relates to crop germplasm resource fields, specifically, being a kind of using hyperoxic conditions acceleration Seed Aging
Seed after method, apparatus and thus obtained aging.
Background technique
Seed Aging refers to seed vitality decline of seed during preservation, and aging can be divided into natural aging and artificial old
Change.The test of artificial acceleration Seed Aging is widely used in seed research field, which can be predicted seed vitality and resistant storage properties
Deng.Artificial acceleration Seed Aging test method is varied, and method commonly used at present is high temperature and humidity method, this method requirement
For temperature at 40-50 DEG C, relative humidity (relative humidity, RH) is 100%.It reports for example, Kong Zhi has etc. using high
The method of warm high humidity artificial accelerated aging, wherein to multiple wheat lines in 90% relative humidity and 40,45,50,55,60 DEG C of items
0,2,4,6 and 8d is handled under part respectively, the results showed that wheat can be shorter with 90% relative humidity and 55 DEG C of high-temperature process
Reaching optimal aging effect in time, (" artificial accelerated aging is to wheat seed CAT, POD, SOD activity and soluble protein
The influence of content " in October, 2010, Chinese grain and oil journal the 10th phase of volume 25).
In tropical deterioration seed at seed under hot and humid environment, as ageing time extends, seed aqueous
Amount is gradually in rising trend, and the seed moisture content after aging before aging compared to that can obviously increase, for example, corn seed is 45
DEG C, under the conditions of 100%RH after aging 2 days, water content is up to 20%.And under normal circumstances, seed is used to carry out compared with low water content
It saves, to inhibit the Physiological and Biochemical Metabolism under the conditions of high-moisture, such as it is 3-10% that mid-term library, which saves seed moisture content range,.
It is well known that the Physiological and Biochemical Metabolism approach in seed cell is with metabolic activity, difference due to water content difference is huge.Therefore, high
The ageing results of warm high humidity can not reflect the Seed Aging under preservation condition completely.Thus urgently finding can be in low water content
The method of quick aging seed makes its ageing results be more nearly the aging under preservation condition.
Summary of the invention
To solve at least partly technical problem in the prior art, the present invention provides one and accelerates seed old using hyperoxic conditions
The scheme of change.Specifically, the present invention includes the following contents.
The first aspect of the present invention provides the method for accelerating Seed Aging comprising seed is made to be in the step of confined space
Suddenly, wherein the volumetric concentration for controlling oxygen in the confined space is 90% or more, humidity is 50%RH or less and temperature is 40-
50℃。
Preferably, before accelerated ageing control seed water content to 3% by up to 10% (based on weight in wet base) below.
Preferably, loading of the seed in confined space makes the total volume of seed and the body in extraseminal space
The ratio between product is 1:5-1:2.
Preferably, the pressure in the confined space is normal pressure.
Preferably, the confined space is bottle inner space, the method includes making seed be placed in bottom of bottle portion, in bottom of bottle portion
It is passed through pure oxygen and bottle inner air is replaced as oxygen, when bottle mouth position oxygen volumetric concentration is 90-100%, bottleneck is sealed, by institute
It states bottle and is placed at 40-50 DEG C and carry out aging process.
Preferably, the seed is crop seeds.
Preferably, the time required to the Seed Aging to 50% germination percentage it is 50 days or less.
Preferably, the total germination percentage of the seed reduces by 15% or more compared with air controls group, and wherein air controls group is
Refer to remaining condition test group identical with the method for the invention other than being air in confined space.
The second aspect of the present invention provides a kind of device for accelerating Seed Aging comprising ontology, oxygen generator and temperature
Spend controller；Wherein, the inside confined space that the ontology has shell and is made of it, the ontology is for accommodating described kind
Son；The oxygen generator is set as the bottom that oxygen outlet is located at the ontology, to allow oxygen from internal confined space
Bottom enter the ontology in；The temperature controller is configured to control temperature within the scope of 40-50 DEG C.Optionally,
Temperature controller may also be configured to be able to detect intrinsic temperature.Optionally, accelerating Seed Aging device further includes humidity control
Device processed.It is 50%RH hereinafter, it is preferred that 20%-40%RH that the humidity controller, which is configured to the humidity of control inner space,.
Preferably, accelerate Seed Aging device can also humidity in detection body.
The third aspect of the present invention provides a kind of Aged Seeds, through the invention the side of the acceleration Seed Aging
Method obtains.
The present invention can the directly quick aging under low water content state.It on the one hand can be preferably using method of the invention
Ageing process of the simulation seeds under preservation condition, each trait expression and routine of seed after another aspect aging in germination period
Aging method is almost the same.Thus, the method that the present invention can be used as seed quick aging under the conditions of low water content is applied to section
It learns in research.
Detailed description of the invention
A kind of exemplary schematic device for accelerating Seed Aging of Fig. 1.
Description of symbols:
1- storage bottle, 2- oxygen tank, 3- oxygen analyser, 4- seed.
Specific embodiment
The existing various exemplary embodiment that the present invention will be described in detail, the detailed description are not considered as to limit of the invention
System, and it is understood as the more detailed description to certain aspects of the invention, characteristic and embodiment.
It should be understood that it is to describe special embodiment that heretofore described term, which is only, it is not intended to limit this hair
It is bright.In addition, for the numberical range in the present invention, it is thus understood that specifically disclose the range upper and lower bound and they it
Between each median.Median and any other statement value in any statement value or stated ranges or in the range
Lesser range is also included in the present invention each of between interior median.These small range of upper and lower bounds can be independent
Ground includes or excludes in range.
Unless otherwise stated, all technical and scientific terms used herein has the routine in field of the present invention
The normally understood identical meanings of technical staff.Although the present invention only describes preferred method and material, of the invention
Implement or also can be used and similar or equivalent any method and material described herein in testing.The institute mentioned in this specification
There is document to be incorporated by reference into, to disclosure and description method relevant to the document and/or material.It is incorporated to any
When document conflicts, it is subject to the content of this specification.Unless otherwise stated, " % " is the percentage based on weight.
" aging " of the present invention, which refers to, to be reduced seed bio-active, leads to the irreversible of its devitalization and germination
Variation, is the process of occurrence and development along with the extension of the storage of seeds time.Currently, the mechanism about Seed Aging is still not
It is clear.
" accelerated ageing " of the present invention refers to that carrying out accelerated ageing processing to seed under the conditions of artificial keeps seed living
The process that power is lost rapidly, can to study the deterioration process for taking a long time to generate under field conditions (factors) in the short time,
It is the effective way for studying seed deterioration rule.
" seed " of the invention refers to any type seed, the preferably seed of angiosperm, and more preferable grass family, pulse family are planted
Species.Seed of the invention includes crop seeds, and the example includes but is not limited to soya seeds, wheat seed, rice seed
Son, peanut seed, corn seed, rapeseed oil seed etc..
Seed Inspection of the invention is with reference to international Seed Inspection association (ISTA) Seed Inspection regulation, so that it is raw to obtain seed
Vigor related data.
[method for accelerating Seed Aging]
The first aspect of the present invention provides the method for accelerating Seed Aging, sometimes referred to as " method of the invention ".The present invention
Method include the steps that seed is made to be in confined space, wherein control confined space in oxygen volumetric concentration be 90%, it is wet
Degree is 50%RH or less and temperature is 40-50 DEG C.
Confined space of the invention refers to provide the space with extraneous relatively independent environment when Seed Aging.Confined air
Between size and shape do not limit, as long as the seed for needing aging can be accommodated, while being capable of providing necessary remaining space i.e.
It can.Preferably, the size of confined space is 4-10 times, preferably 5-8 times, more preferable 5-6 times of seed total volume for needing aging.
Confined space of the invention is needed in aging in high oxygen condition.Hyperoxia of the invention refers to oxygen volumetric concentration
It is 90% or more, preferably 95% or more, more preferable 96% or more, further preferred 98% or more, further preferably 100% (that is, pure oxygen
State).Present invention discover that 90% or more oxygen volumetric concentration can greatly accelerate the aging of seed.If oxygen volumetric concentration
Lower than 90%, then Seed Aging speed is substantially reduced.If oxygen volumetric concentration is reduced to 21%, Seed Aging almost and from
Right aging speed is suitable.High oxygen condition of the invention can obtain in any manner.For example, provided by hyperbaric oxygen gas tank, or by
Oxygen generator is generated and is provided.
Humid control in confined space of the invention is 50%RH or less." humidity " of the invention refers to relative humidity,
Unless otherwise indicated.Need high humidity different from existing artificial acceleration Seed Aging, damp condition and seed of the invention
Humidity when storage is consistent, to be more advantageous to ager process when imitating seed storage or saving.Relative humidity of the invention
Generally 10%-50%, it is highly preferred that relative humidity of the invention is 20%-40%, for example, 20%, 25%, 30% and
35% etc..
Seed moisture content control of the invention is 3%-10%.Signified " water content " of the invention refers to based on weight in wet base
Water content, unless otherwise indicated.Water content items different from the existing common high-moisture of artificial acceleration Seed Aging, of the invention
Part is consistent with water content when seed storage, to be more advantageous to ager process when imitating seed storage or saving.Preferably,
Water content of the invention is 3%-10%, it is highly preferred that water content of the invention is 4%-9%, for example, 5%, 6%, 7% and
8% etc..
Temperature in confined space of the invention need to generally control within the scope of 40-50 DEG C, and preferably 45-58 DEG C, more preferably
45-55℃.The temperature the low more is unfavorable for the acceleration of aging.On the other hand, if temperature is excessively high, change institute beyond seed physiology
The temperature needed is unfavorable for imitating seed natural aging process.Needed for temperature of the invention can be for example, by realizing in ageing oven
Temperature range.
Pressure in confined space of the invention is generally normal pressure.Normal pressure of the invention refers to close to atmospheric pressure, that is,
Within the scope of 0.1 ± 0.05MPa, within the scope of preferably 0.1 ± 0.02MPa.It is under normal pressure when seed natural aging or seed storage
It carries out, control normal pressure is in confined space of the present invention so as to the ager process under more preferable simulation natural aging or condition of storage.
If hypertonia, it may be more advantageous to accelerated ageing, but cannot be simulated well under natural aging or condition of storage
Ager process.
Confined space of the invention is further preferably in dark state, to be more advantageous to old under simulation seeds condition of storage
Change.
Seed of the invention generally require controlled before accelerated ageing its water content to 3% with up to 10% hereinafter, into one
Step preferably during the entire process of accelerated ageing, control the water content of seed 3% with up to 10% hereinafter, more preferably 4%
With up to 9% hereinafter, for example, 8% etc..The loading of seed of the invention in confined space is not particularly limited, and preferably it is filled out
Charge makes the ratio between the total volume of seed and the volume in extraseminal space be 1:5-1:2, for example, 1:5-1:4.
In certain embodiments, confined space of the invention is bottle inner space, acceleration Seed Aging of the invention
Method includes that seed is made to be placed in bottom of bottle portion, is passed through pure oxygen in bottom of bottle portion and bottle inner air is replaced as oxygen, when bottle mouth position oxygen
When volumetric concentration is 90-100%, bottleneck is sealed, the bottle is placed at 40-50 DEG C and carries out aging process.
In the exemplary method for accelerating Seed Aging comprising following steps:
(1) seed material requirement: seed material water content is within 10%.
(2) it manufactures oxygen environment: the seed of appropriate volume being put into sample bottle, oxygen is passed through, is guaranteed using oxygen analyser
Oxygen concentration is in 90-100% or more and stablizes a period of time, then seals, and the volume and seed overhead volume ratio of seed are 1:
5-1:4；
(3) ageing oven temperature setting: temperature is arranged in 40-45 DEG C in advance.
(4) high temperature Artificial ageing: will fill seed-bearing sample bottle and be put into ageing oven, aging certain time.
(5) detect seed vigor: Seed germination is done in the seed taking-up after aging, to detect seed vigor.
[device for accelerating Seed Aging]
The second aspect of the present invention provides the device for accelerating Seed Aging, sometimes referred to as the device of the invention.Of the invention
Device is the device of method for carrying out the present invention, generally comprises ontology, oxygen generator, temperature controller, optionally
It further include humidity controller.The device of the invention can be midget plant.For example, the midget plant used in laboratory.The present invention
Device can also be large-scale plant.In certain embodiments, apparatus of the present invention are automation equipment.
Ontology of the invention is and to provide the main structure of relatively independent confined space for accommodating seed, general
With shell and the inside confined space being made of it.The not specific restriction of the material of shell, preferably gas impermeable material, such as can be with
It is glass, duroplasts, ceramics etc..
Oxygen generator of the invention is set as the bottom that oxygen outlet is located at ontology, to allow oxygen closed from inside
The bottom in space enters in ontology.The example of oxygen generator of the invention includes hyperbaric oxygen gas tank, oxygen generator etc..
Temperature controller of the invention is set as the temperature in control confined space within the scope of 40-50 DEG C.Preferably, also
It is able to detect intrinsic temperature, and carries out temperature control as needed.Humidity controller of the invention is configured to control
Humidity is 50%RH hereinafter, it is preferred that 20%-40%RH.Preferably, additionally it is possible to the humidity in detection body.Temperature controller and wet
Device or commercial products known in the art can be used in degree controller.
The device of the invention may also include oxygen measurer, be used to directly or indirectly detect the oxygen content in confined space.
[Aged Seeds]
The third aspect of the present invention provides a kind of Aged Seeds, is prepared by means of the present invention.Of the invention
Aged Seeds can be applied in scientific research.
Embodiment 1
The present embodiment is the device for implementing the method for the present invention for accelerating Seed Aging.As shown in Figure 1, of the invention
Device include storage bottle 1, oxygen tank 2 and oxygen analyser 3.Seed 4 is placed in storage bottle.Oxygen tank by conduit by pure oxygen from
Bottom of bottle portion introduces oxygen, to replace the air in bottle, oxygen analyser detects the concentration of bottle mouth position oxygen, when volumetric concentration reaches
When 90% or more, oxygen catheter, sealed opening are drawn back.In addition, the device of the invention further includes ageing oven, it is temperature of the invention
Control device is spent, temperature can be controlled in 40-50 DEG C of range.In addition, the device of the invention further includes humidity controller, for examining
Surveying and controlling the humidity in bottle is 50% or less.
Embodiment 2
Following experiment is carried out using device described in embodiment 1.
Seed material: soybean 8213, rice T be excellent 5128, wheat 4185, initial germination percentage is respectively 97.5%, 90.5%,
83.0%, seed moisture content is on 8-10% (weight in wet base basis).
Test process: each variety seeds are respectively put into the jaw bottle of 50mL, the air in a portion jaw bottle
It is replaced with pure oxygen, 95% or more oxygen concentration control, as high oxygen treatment；It is then air in a part of jaw bottle, as control
Group, oxygen concentration are calculated as 21%.After seed packaging, it is placed in 45 ± 1 DEG C of ageing ovens and carries out artificial ageing, ageing time 40
It.Each 3-4 repetition of processing, it is each to repeat 10g seed.
The seed for taking out aging balances for 24 hours at room temperature, germination test is then done, with reference to international Seed Inspection association
(ISTA) Seed Inspection regulation obtains Aged Seeds items germination index.In Seed germination, wheat uses to germinate on paper, temperature
It is 20 DEG C, every repetition 50,4 repetitions；Rice paddy seed on paper using germinateing, and temperature is 28 DEG C, every repetition 50,4 repetitions；
Soya seeds are 25 DEG C using paper+sponge germination, temperature, every repetition 25,4 repetitions.Obtained germination the results are shown in Table 1.
It germinates after 1 Seed Aging of table result
Test results and analysis: as shown in table 1, each crop seed under different oxygen concentrations after the aging identical time,
The vigor decline degree of seed be it is different, seed vitality reduces fast under hyperoxic conditions, and seed is living when collating condition aging
Power reduces slow, the total germination percentage of seed 20-30% low compared with the control under hyperoxic conditions, and normal seedling is low 30% left
The right side, the difference for even having 50% or so of soybean, this shows that hyperoxic conditions can be used as one kind and accelerate under the conditions of low water content
The method of Seed Aging.
Embodiment 3
Following experiments are carried out using the device of embodiment 1.
Seed material: soya seeds
Test process: comparison the method for the present invention and other Seed Aging methods, including room temperature natural aging is (in Beijing room
Outside), traditional high temperature and humidity and high temperature ageing method, to the aging effect of soya seeds.For convenience of comparison, under the conditions of statistics is each
Aging to 50% germination percentage time, by Seed Aging speed, to the analog capability of seed natural aging and to holding conditions
The Pros and Cons of the analog capability evaluation each method of lower Seed Aging.
2 soya seeds difference aging techniques contrast table of table
Test results and analysis: from comparative analysis in table 2 it is known that under natural aging mode, Seed Aging speed is slow,
Aging even needs 1 year or so to 50% germination percentage, it is difficult to carry out Seed Aging research, and because of seed moisture content during saving
It can be fluctuated with air humidity, this method aging can be poor to the simulation under the conditions of cryopreservation.
Tropical deterioration mode percentage of seedgermination most can be reduced to 50% at 7-14 days fastly, hence it is evident that improve Seed Aging
Speed, but since ageing process is frequently with 75% to 100% super-humid conditions, seed moisture content is excessively high after aging, especially exists
Seed moisture content may be up to 20% when aging under the conditions of 100%, with seed moisture content under the conditions of natural aging and cryopreservation
Gap is huge, and since water content difference causes seed intracellular metabolite approach difference huge, thus analog capability is poor.
High temperature process aging uses sealed package, and seed moisture content can be with any range, thus can choose and store with low temperature
Water content levels similar in hiding or natural aging carry out aging, therefore its analog capability is better than high temperature and humidity method, but old
It is slower to change speed, when carrying out aging for example, by using 8% water content, needs 160 days germination percentages that can just be reduced to 50%.Using this hair
Bright method accelerated ageing seed, Seed Aging to then foreshortening to 50 days or so the time required to 50% germination percentage, on the other hand compared with
Good simulation ageing state of the seed under natural conditions and holding conditions, thus the method for the present invention can accelerate Seed Aging simultaneously
And facilitate the further research after Seed Aging.
Without departing substantially from the scope or spirit of the invention, the specific embodiment of description of the invention can be done more
Kind improvements and changes, this will be apparent to those skilled in the art.Other realities obtained by specification of the invention
Applying mode for technical personnel is apparent obtain.Present specification and embodiment are merely exemplary.
Claims (8)
1. a kind of method for accelerating Seed Aging, which is characterized in that include the steps that seed is made to be in confined space, wherein controlling
The volumetric concentration of oxygen is 90% or more in the confined space, humidity is 50%RH or less and temperature is 40-50 DEG C.
2. the method according to claim 1 for accelerating Seed Aging, which is characterized in that control containing for seed before accelerated ageing
Water 3wt% is up to 10wt% or less.
3. the method according to claim 2 for accelerating Seed Aging, which is characterized in that the seed is in confined space
Loading makes the ratio between the total volume of seed and the volume in extraseminal space be 1:5-1:2.
4. the method according to claim 3 for accelerating Seed Aging, which is characterized in that the pressure in the confined space is
Normal pressure.
5. the method according to claim 3 for accelerating Seed Aging, which is characterized in that the confined space is empty inside bottle
Between, the method includes making seed be placed in bottom of bottle portion, pure oxygen is passed through in bottom of bottle portion, bottle inner air is replaced as oxygen, work as bottleneck
When place's oxygen volumetric concentration is 90-100%, bottleneck is sealed, the bottle is placed at 40-50 DEG C and carries out aging process.
6. the method according to claim 1 for accelerating Seed Aging, which is characterized in that the seed is crop seeds.
7. a kind of device for accelerating Seed Aging, which is characterized in that including ontology, oxygen generator and temperature controller；Wherein,
The inside confined space that the ontology has shell and is made of it, the ontology is for accommodating the seed；The oxygen hair
Raw device is set as the bottom that oxygen outlet is located at the ontology, to allow oxygen from described in the entrance of the bottom of internal confined space
In ontology；The temperature controller is configured to control the temperature of internal confined space within the scope of 40-50 DEG C.
8. a kind of Aged Seeds are obtained by the method according to claim 1-6 for accelerating Seed Aging.
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| CN201910298989.1A CN109804751A (en) | 2019-04-15 | 2019-04-15 | Accelerate the method, apparatus and gained Aged Seeds of Seed Aging |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111492745A (en) * | 2020-04-08 | 2020-08-07 | 吉林省农业科学院 | Method for detecting corn storage tolerance |
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| CN106717244A (en) * | 2016-11-15 | 2017-05-31 | 黄家七 | A kind of low-temperature storage method of corn seed |
| CN107710953A (en) * | 2017-10-30 | 2018-02-23 | 蚌埠农兴现代农业专业合作社 | A kind of storage practice for improving Germination of Soybean Seed rate |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106717244A (en) * | 2016-11-15 | 2017-05-31 | 黄家七 | A kind of low-temperature storage method of corn seed |
| CN107710953A (en) * | 2017-10-30 | 2018-02-23 | 蚌埠农兴现代农业专业合作社 | A kind of storage practice for improving Germination of Soybean Seed rate |
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| E.H.ROBERTS ET AL.: ""The influence of temperature, moisture, and oxygen on period of seed biability in barley, broad beans, and peas"", 《ANNALS OF BOTANY》 * |
| OHLROGGE JB ET AL.: ""Oxygen-dependent aging of seeds"", 《PLANT PHYSIOL》 * |
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Application publication date: 20190528 |