CN207072949U - A kind of total plate count automatic detection device - Google Patents
A kind of total plate count automatic detection device Download PDFInfo
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- CN207072949U CN207072949U CN201720806525.3U CN201720806525U CN207072949U CN 207072949 U CN207072949 U CN 207072949U CN 201720806525 U CN201720806525 U CN 201720806525U CN 207072949 U CN207072949 U CN 207072949U
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- plate count
- liquid
- total plate
- detection device
- automatic detection
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Abstract
The utility model discloses a kind of total plate count automatic detection device, including reagent component, microbiological incubator, support and controller；The support is set up in above the reagent component and microbiological incubator, and liquid relief component and gripper assembly are movably set with the support；The reagent component includes reagent bottle, dilution unit and the conical flask equipped with culture medium equipped with testing sample；Some culture dishes and the camera for gathering image information in culture dish are provided with the microbiological incubator；The controller electrically connects with liquid relief component, gripper assembly and the microbiological incubator, and the image information gathered by analyzing the camera calculates the total plate count of testing sample.
Description
Technical field
It the utility model is related to a kind of total plate count automatic detection device.
Background technology
The detection of microbe colony sum can be used for judging the degree and health of its microbial contamination in food, medicine
Quality, it reflects whether food meets hygienic requirements in process of production with medicine, to make appropriate defend to test sample
It is raw to learn evaluation, total plate count number indicate the quality of product quality to a certain extent, therefore, measure food with medicine
Microbe colony sum it is significant.
At present, the detection method of microbe colony sum must be by food and medicine《Country of People's Republic of China (PRC) mark
It is accurate》It is required that standardized operation is carried out, to obtain standard, testing result uniformly, accurately, fair, just.However, in microorganism
All it is to use manually to be detected in laboratory all the time, this is just to the skill of testing staff in the detection process of total plate count
Art, dexterity and exquisiteness propose very high request, even the minimum nonstandard operation of testing staff one or operated
The extraneous factor interference that one minimum in journey is likely to have influence on the accuracy of testing result.Therefore, testing result is objective
Property is queried sometimes unavoidably.Therefore, there is an urgent need to a kind of automatic detection system can replace manual operation for industry, not only reduce
Dependence of the detection process to manpower technical ability, and artificial various subjective or objective factor influences can be reduced, allow testing result more
Accurately, more standard, with more masses convince power.
Utility model content
The purpose of this utility model is in place of overcome the deficiencies in the prior art, there is provided a kind of total plate count automatic detection
Device.
Technical scheme is used by the utility model solves its technical problem：
Provide a kind of total plate count automatic detection device, including reagent component, microbiological incubator, support and be programmed with
The controller of microbe colony sum calculation procedure；
The support is set up in above the reagent component and microbiological incubator, and liquid relief is movably set with the support
Component and gripper assembly；
The reagent component includes reagent bottle, dilution unit and the conical flask equipped with culture medium equipped with testing sample；Institute
Stating dilution unit includes some test tubes and rack for test tube；
Some culture dishes and the camera for gathering image information in culture dish are provided with the microbiological incubator；
The controller electrically connects with liquid relief component, gripper assembly and the microbiological incubator, the controller control
Liquid relief component pipettes liquid in reagent component into culture dish, is covered on the controller control gripper assembly opening and closing culture dish,
Testing sample the image information of the control assembly control camera collection and processing, calculates after culture period and treats test sample
The total plate count of product.
In a preferred embodiment of the present utility model, the casing of the microbiological incubator divides successively from top to bottom
For culturing room, collection room and temperature controlled compartment；The culturing room is made up of perisporium, the upper lid of casing with shaking table, and the shaking table is positioned at training
Room bottom is supported, for separating the culturing room and collection room；The upper lid is electrically connected with the controller with shaking table.
In a preferred embodiment of the present utility model, at least eight culture dish is provided with the shaking table.
In a preferred embodiment of the present utility model, the collection interior is provided with to be electrically connected with the controller
Camera.
In a preferred embodiment of the present utility model, it is provided with the temperature controlled compartment for controlling microculture
The constant temperature gas bath generating means of the temperature inside the box.
In a preferred embodiment of the present utility model, the culture dish quantity is at least 8.
In a preferred embodiment of the present utility model, the liquid relief component includes the liquid-transfering gun that quantity is 2, described
Liquid-transfering gun includes 1mL liquid-transfering guns and 20mL liquid-transfering guns.
In a preferred embodiment of the present utility model, the gripper assembly includes folder lid device.
In a preferred embodiment of the present utility model, in addition to thermostat water bath, for placing the conical flask.
In a preferred embodiment of the present utility model, in addition to vortex oscillator, it is arranged under the rack for test tube
Side, for shaking up sample liquid in test tube.
Compared with background technology, it has the following advantages that the technical program：
(1) detection operation is controlled by controller, reduces dependence of the detection process to manpower technical ability, avoid artificial
Operational error；
(2) the utility model is novel in design, easy to use, and intelligence degree is high, can automation mechanized operation, grasp detection process
Work is simplified, automated, unitizing.
Brief description of the drawings
The utility model is described in further detail with reference to the accompanying drawings and examples.
Fig. 1 is a kind of structural representation of total plate count automatic detection device；
Fig. 2 is the structural representation of microbiological incubator.
Embodiment
Content of the present utility model is illustrated below by embodiment：
It refer to Fig. 1, a kind of total plate count automatic detection device, including reagent component, microbiological incubator, the and of support 3
It is programmed with the controller 4 of microbe colony sum calculation procedure；The microbiological incubator is schematically shown as by Fig. 1 for convenience of description
Top view, in practical operation, microbiological incubator, reagent component are placed on ultra-clean operating desk；
The support 3 is set up in above the reagent component and microbiological incubator, and shifting is movably set with the support 3
Liquid component 32 and gripper assembly；The liquid relief component 32 and gripper assembly are hung on support, and its scope of activities covers the examination
Agent component and microbiological incubator；
The reagent component includes reagent bottle 11, dilution unit and the conical flask equipped with culture medium equipped with testing sample
15；The dilution unit includes some test tubes 12 and rack for test tube 13；
Fig. 2 is refer to, some culture dishes 25 are provided with the microbiological incubator and are schemed for gathering in culture dish 25
As the camera 22 of information；
The controller 4 electrically connects with liquid relief component 32, gripper assembly and the microbiological incubator, the controller 14
Liquid relief component 32 is controlled to pipette the liquid in reagent component into culture dish 25, the controller 14 controls gripper assembly 31 to be opened and closed
Covered on culture dish 25, for testing sample after culture period, the control assembly 14 controls the image information of the collection of camera 22 simultaneously
Processing, calculate the total plate count of testing sample.
In the present embodiment, the casing of the microbiological incubator is divided into culturing room 26, collection room 27 from top to bottom
With temperature controlled compartment 28；The culturing room 26 is made up of perisporium, the upper lid 24 of casing with shaking table 23, and the shaking table 23 is located at culturing room 26
Bottom, for separating the culturing room 26 and collection room 27；The upper lid 24 electrically connects with shaking table 23 with controller 4.It is described to shake
At least eight culture dish 25 and the groove of culture dish 25 are provided with bed 23.It is provided with the collection room 27 and is electrically connected with the controller 4
The camera 22 connect.
In the present embodiment, it is at least 8 for the reliability of increase detection limit and inspection result, the quantity of culture dish 25.
It is suitable for conditions such as the temperature needed for guarantee microculture, humidity, it is provided with the temperature controlled compartment for controlling
The constant temperature gas bath generator 21 of temperature in microbiological incubator.
In the present embodiment, the liquid relief component includes the liquid-transfering gun that quantity is 2, and the liquid-transfering gun includes 1mL liquid-transfering guns
With 20mL liquid-transfering guns.
In the present embodiment, the gripper assembly includes folder lid device 31, is mainly used in gripping culture dish 25 and covers.
In the present embodiment, in addition to thermostat water bath 16, for placing the conical flask 15, it is ensured that conical flask 15
In culture medium maintain certain temperature.
In the present embodiment, in addition to vortex oscillator 14, the lower section of rack for test tube 13 is arranged at, for making sample in test tube 12
Liquid is evenly distributed.
The automatic detection to microbe colony sum in food, medicine, its implementation are realized using the present embodiment device
For：
(1) early-stage preparations：1 set of said apparatus is taken, including the reagent bottle 11 equipped with sample liquid, sample liquid is by solid, half
The testing sample of solid or liquid pre-processes 1 formed:The even liquid of 10 samples；12 6, test tube, numbering 1-6；Sterile petri dish 25
Eight, numbering 1-8；Conical flask 15 equipped with PCA culture mediums is put into the thermostat water bath 16 for setting temperature to be 46 DEG C；
Testing staff strictly carries out various equipment by the requirement in national standard, sterileization of reagent is handled.In the present apparatus
Reagent bottle, 1-6 test tubes, equipped with PCA culture mediums conical flask, culture dish, the 1mL glass pipette tips of supporting liquid-transfering gun and 20mL
Glass gun is first-class to carry out the preceding packaging that sterilizes on request, then in the sterilizing 20 minutes of 121 degree of high-pressure steam sterilizing pan, most after drying
Dried in case；By the above-mentioned sterilized equipment do not unpacked and reagent, together with the present apparatus, additional appropriate sterile saline
(commercially available), is together placed in superclean bench；The cleaning of unlatching superclean bench, progress operating environment and its interior article, air-flow etc.,
Dissipation；Testing staff is assessed sample pollution situation in advance, selects the even liquid of sample of 2~3 dilution gradients to carry out microorganism
Total plate count is detected, and this parameter setting is carried out in the controller of the present apparatus.
(2) testing sample is handled：
1. testing staff starts said apparatus, liquid-transfering gun is controlled accurately to pipette 9mL sterile physiological salt respectively by controller 4
Water is in 1-6 test tubes 12；
2. control folder lid device 31 picks up No. 1 culture dish lid；
3. control 1mL liquid-transfering guns accurately pipette 1mL sterile salines in center in No. 1 culture dish 25, as sky
White control；
4. control folder lid device 31 covers No. 1 culture dish lid；
5. repeat step 2. -4. in No. 2 culture dishes 25 center move plus 1mL sterile salines, as blank pair
According to；
6. 1mL liquid-transfering guns are controlled accurately to pipette the even liquid of sample in 1mL reagent bottles 11 in No. 1 test tube 12 by controller 4
In；
Liquid in No. 1 test tube 12 is mixed 7. control starts vortex oscillator 14, is made 1:The even liquid of 100 samples, and
Close vortex oscillator 14 automatically afterwards；
8. controlling the glass pipette tips of 1mL liquid-transfering guns to insert in No. 1 liquid of test tube 12, blown by inhaling repeatedly in No. 1 test tube 12
Liquid 5 times, rinse is carried out to the glass pipette tips of 1mL liquid-transfering guns；
9. repeat step 6. -8., respectively in No. 2 test tubes 12 be made 1:The even liquid of 1000 samples, it is made in No. 3 test tubes 12
1:The even liquid of 10000 samples, 1 is made in No. 4 test tubes 12:The even liquid of 100000 samples；
10. controlling the glass pipette tips of 1mL liquid-transfering guns to insert in No. 4 liquid of test tube 12, No. 4 examination intraluminal fluids are blown by inhaling repeatedly
Body 5 times, rinse is carried out to the glass pipette tips of 1mL liquid-transfering guns；
Repeat step 2. -4. in No. 3 and No. 4 culture dishes 25 center move plus 1mL4 test tubes in 1:100000 samples
The even liquid of product；
Repeat step 6. -8., respectively in No. 5 test tubes 12 be made 1:The even liquid of 1000000 samples, in No. 6 test tubes 12
It is made 1:The even liquid of 10000000 samples；
In stepWhile, repeat step 2. -4., respectively in No. 5 and No. 6 culture dishes 25 center move plus
1 in 1mL5 test tubes:The even liquid of 1000000 samples；Move and add in No. 6 test tubes of 1ml in center in No. 7 and No. 8 culture dishes 25
1:The even liquid of 10000000 samples；
The shaking table that control starts in microbiological incubator is gently shaken clockwise；
Control folder lid device 31 picks up No. 1 culture dish lid；
Control 20mL liquid-transfering guns pipette the PCA culture mediums in 20mL conical flasks, are slowly added into No. 1 culture dish centre bit
Put, and in the presence of shaking table, PCA cultures is well mixed based on the even liquid of sample in culture dish 25；
Control folder lid device 31 covers No. 1 culture dish lid；
Repeat stepPCA culture mediums are slowly added into 2-8 culture dishes 25, and in the presence of shaking table,
PCA cultures are made to be well mixed based on the even liquid of sample in culture dish 25；
The shaking table that control is closed in microbiological incubator shakes, and stands cooling 20min, cultivates PCA in culture dish 25
Base solidifies；
The upper lid of microbiological incubator is closed in control；
(3) culture and detection：
Above-mentioned culture dish 25 is placed in microbiological incubator and cultivates the scheduled time, controller 4 can be to perseverance in incubation
The keying of wet bath generator 21 and temperature are configured；After the scheduled time, control starts camera 22 and No. 1-8 is cultivated
Ware 25 is taken pictures, and the photographic intelligence collected is fed back into controller 4；Microbe colony is programmed with the controller 4
Total calculation procedure, every gram or every milliliter can be calculated automatically and detects the microbe colony sum contained in sample.
Display screen is provided with controller 4, result of calculation can be directly displayed, can also be with printer, circumscribed USB equipment
Or computer connection, printing or transmission result of calculation.
It is described above, only the utility model preferred embodiment, therefore the utility model implementation can not be limited according to this
Scope, i.e., the equivalent changes and modifications made according to the utility model patent scope and description, it is new all should still to belong to this practicality
In the range of type covers.
Claims (10)
- A kind of 1. total plate count automatic detection device, it is characterised in that：Including reagent component, microbiological incubator, support and volume It is formed with the controller of microbe colony sum calculation procedure；The support is set up in above the reagent component and microbiological incubator, and liquid relief component is movably set with the support With for gripping the gripper assembly covered on culture dish；The reagent component includes reagent bottle, dilution unit and the conical flask equipped with culture medium equipped with testing sample；It is described dilute Releasing unit includes some test tubes and rack for test tube；Some culture dishes and the camera for gathering image information in culture dish are provided with the microbiological incubator；The controller electrically connects with liquid relief component, gripper assembly and the microbiological incubator, and the controller controls liquid relief Component pipettes liquid in reagent component into culture dish, is covered on the controller control gripper assembly opening and closing culture dish, to be measured Sample the image information of the control assembly control camera collection and processing, calculates testing sample after culture period Total plate count.
- A kind of 2. total plate count automatic detection device according to claim 1, it is characterised in that：The microbiological incubator Casing be divided into culturing room, collection room and temperature controlled compartment from top to bottom；The culturing room by casing perisporium, upper lid and shaking table Composition, the shaking table are located at culturing room bottom, for separating the culturing room and collection room；The upper lid and shaking table and controller Electrical connection.
- A kind of 3. total plate count automatic detection device according to claim 2, it is characterised in that：It is provided with the shaking table At least eight culture dish.
- A kind of 4. total plate count automatic detection device according to claim 3, it is characterised in that：The indoor setting of collection There is the camera electrically connected with the controller.
- A kind of 5. total plate count automatic detection device according to claim 1, it is characterised in that：The culture dish quantity is extremely It is 8 less.
- A kind of 6. total plate count automatic detection device according to claim 2, it is characterised in that：Set in the temperature controlled compartment There is the constant temperature gas bath generating means for controlling temperature in microbiological incubator.
- A kind of 7. total plate count automatic detection device according to claim 1, it is characterised in that：The liquid relief component includes Quantity is 2 liquid-transfering gun, and the liquid-transfering gun includes 1mL liquid-transfering guns and 20mL liquid-transfering guns.
- A kind of 8. total plate count automatic detection device according to claim 1, it is characterised in that：The gripper assembly includes Press from both sides lid device.
- A kind of 9. total plate count automatic detection device according to claim 1, it is characterised in that：Also include water bath with thermostatic control Pot, for placing the conical flask.
- A kind of 10. total plate count automatic detection device according to claim 1, it is characterised in that：Also include vortex oscillation Device, it is arranged at below the rack for test tube, for making sample liquid in test tube be evenly distributed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720806525.3U CN207072949U (en) | 2017-07-05 | 2017-07-05 | A kind of total plate count automatic detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720806525.3U CN207072949U (en) | 2017-07-05 | 2017-07-05 | A kind of total plate count automatic detection device |
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| Publication Number | Publication Date |
|---|---|
| CN207072949U true CN207072949U (en) | 2018-03-06 |
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| CN201720806525.3U Active CN207072949U (en) | 2017-07-05 | 2017-07-05 | A kind of total plate count automatic detection device |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113122436A (en) * | 2021-03-29 | 2021-07-16 | 中标华谱(山东)智能科技有限公司 | Culture dish manufacturing device and method for food microorganism testing |
| CN114317229A (en) * | 2021-12-30 | 2022-04-12 | 驻马店市疾病预防控制中心 | Multifunctional microorganism automatic inspection equipment |
| CN114456922A (en) * | 2022-01-20 | 2022-05-10 | 江苏禹视科技有限公司 | Bacterial colony culture detection device and method |
-
2017
- 2017-07-05 CN CN201720806525.3U patent/CN207072949U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113122436A (en) * | 2021-03-29 | 2021-07-16 | 中标华谱(山东)智能科技有限公司 | Culture dish manufacturing device and method for food microorganism testing |
| CN114317229A (en) * | 2021-12-30 | 2022-04-12 | 驻马店市疾病预防控制中心 | Multifunctional microorganism automatic inspection equipment |
| CN114456922A (en) * | 2022-01-20 | 2022-05-10 | 江苏禹视科技有限公司 | Bacterial colony culture detection device and method |
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