CN109793803A - A kind of application and preparation method of the substance preventing and treating atherosclerosis - Google Patents
A kind of application and preparation method of the substance preventing and treating atherosclerosis Download PDFInfo
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Abstract
The present invention disclose it is a kind of prevent and treat atherosclerosis substance and its application.Substance of the invention is the unsaponifiable matter of the fatty contents of the seed of rubber tree, can be applied to drug, health food, in food, in particular to it is preventing and treating application in atherosclerosis.Children with childhood obesity pass through daily to take or the edible drug, health food, food for being added to the substance can be obtained good prevention and treatment effect.
Description
Technical field
The invention belongs to biomedicine fields, specifically not being saponified the present invention relates to seeds of rubber tree fatty contents
Object is preventing and treating the application in atherosclerosis.
Background technique
Atherosclerosis (atherosclerosis, As) is a kind of multifactorial disease of complexity, endothelial cell damage,
Inflammation mechanism plays particularly important effect in the occurrence and development of atherosclerosis.It is now recognized that AS includes multiple pathology
Process is damaged by the damage of vascular wall, inner skin cell function, local function obstacle, and sub-endothelial matrix and collagen exposure, blood are small
Plate adhesion and aggregation generates monocyte chemotactic factor and adhesion molecule, blood coagulation and anticoagulation system imbalance, thrombosis and inflammation
Reaction, vascular smooth cell activation, migration, caused by proliferation and extracellular matrix generation etc. are common.This disease is mainly involved medium-sized
Flesh elastic-type artery is led to luminal stenosis, is become fragile with aorta, coronary artery and cerebral artery, and stability decline causes corresponding
Internal organs ischemic, the serious consequences such as infarct.
With the improvement of living standards and living-pattern preservation, coronary heart disease and the incidence of cerebral arterial thrombosis are on gradually
It rises, thus the occurrence risk of caused serious bad cardiovascular and cerebrovascular disease obviously increases, and is in rejuvenation trend.Seriously affect people
The health and social productiveness of labor of class bring heavy financial burden to thousands upon thousands families or even society, everything is tight
The pushing hands of weight adverse events behind is exactly atherosclerosis.
At present a large number of studies show that statins there are pleiotropic effects, become the drug of current most effective treatment AS
One of.Statin is a kind of 3-hydroxyl-3-methylglutaryl coenzyme A A reductase inhibitor, by inhibiting the synthesis of cholesterol to reduce
Blood lipid level plays the role of antiatherosclerosis.However statins prolonged application can produce obvious adverse reaction, especially
It is hepatic disorder, this to a certain degree on limit the clinical prolonged application of such drug.Drug has very big side effect, no
It can be used for a long time, in face of the huge disease incidence of atherosclerosis and being continuously increased for patient, continual exploitation is more effective and without pair
The drug of effect is ailing to reduce patient, while because of drug side-effect bring, other physical impairments just become very urgent for reduction.
Summary of the invention
Some does not prevent and treat the substance of atherosclerosis the purpose of the present invention is to provide a kind of in the prior art,
The application of the unsaponifiable matter of seeds of rubber tree fatty contents.The substance is not being saponified for the fatty contents of the seed of rubber tree
Object can be applied to drug, health food, in food, the prevention and treatment for atherosclerosis.Atherosclerosis is suffered from
Person passes through daily to take or the edible drug, health food, food for being added to the substance can be obtained good prevention and control
Therapeutic effect.
In order to realize above-mentioned purpose of the invention, the present invention provides the following technical solutions:
Seeds of rubber tree fatty contents, the unsaponifiable matter of the seeds of rubber tree fatty contents by being prepared in following methods and
:
Using virgin rubber tree species as raw material, after drying shelling, kernel is prepared, kernel passes through mechanical presses method, extraction
The lipoclastic of seeds of rubber tree is made in one or more of method, supercritical extraction, is original with the lipoclastic of seeds of rubber tree
Material is added excessive potassium hydroxide-ethanol solution and carries out saponification, after saponification terminates, under vacuum conditions, heating
Ethyl alcohol is recycled, one of unsaponifiable matter extractant ether, ethyl acetate, n-hexane, No. 6 solvents are added later and is extracted,
Upper layer of extraction liquid is isolated, under vacuum conditions reheating, recycle extractant, remaining liquid is seeds of rubber tree
The unsaponifiable matter of fatty contents.
Invention also provides the unsaponifiable matter of the seeds of rubber tree fatty contents containing therapeutically effective amount
With the pharmaceutical composition of pharmaceutically acceptable carrier.
And health food, it is commonly used and is carried by the unsaponifiable matter and health food of the seeds of rubber tree fatty contents
Body is formed.Food is made of the unsaponifiable matter and food common carrier of the seeds of rubber tree fatty contents.
It is dynamic in prevention and treatment that the present invention also provides the preparations of the unsaponifiable matter of the seeds of rubber tree fatty contents
Application in the drug of pulse atherosclerosis.
Application as mentioned, wherein the unsaponifiable matter of the seeds of rubber tree fatty contents as sole active at
Point.
Present invention further provides the preparation of the unsaponifiable matter of the seeds of rubber tree fatty contents treatment and
Prevent the application in the health food of dynamic atherosis.
And the preparation of the unsaponifiable matter of the seeds of rubber tree fatty contents is preventing and treating Atherosclerosis
Application in the food of change.
Application as mentioned, wherein the unsaponifiable matter of the seeds of rubber tree fatty contents as sole active at
Point.
With existing for compared with preventing and treating the drug of atherosclerosis, the invention has the following advantages that
1, to provide a kind of completely new substance with atherosclerotic；
2, without side-effects, it can be for being used for a long time with atherosclerotic；
3, safety is good, takes for being not suffering from the ordinary person of atherosclerosis, there is the effect of prevention of arterial atherosis.
Specific embodiment:
Embodiment 1
Using 100kg virgin rubber tree species as raw material, after drying shelling, kernel 40kg is prepared.Kernel material arrives 80- in heat
100 DEG C, then by static extruding, pressure 50MPa, squeezing out 12kg liquid is substance rubber tree species of the invention
Sub- fatty contents.
Using the lipoclastic of 10kg seeds of rubber tree as raw material, 10kg potassium hydroxide-ethanol solution is added and carries out saponification,
The mass percent concentration of potassium hydroxide in ethanol is 22%.After saponification terminates, under 0.01atm vacuum condition, heating
To 120 DEG C of recycling ethyl alcohol；5kg ether is added later to extract as unsaponifiable matter extractant, isolates upper layer of extraction liquid,
Under 0.01atm vacuum condition, 85 DEG C of recycling extractants are heated to, 400g liquid, rubber tree species as of the invention are obtained
The unsaponifiable matter of the fatty contents of son.
1, experimental animal: health purebred New Zealand's male White Rabbit 48, week old is 4-6 weeks, weight (2.24 ± 0.87)
kg.
2, experimental method
2.1, it is grouped: 15 DEG C ~ 20 DEG C of room temperature, drinking water on demand, adaptable fed 1 week, stablize blood lipid level and metabolism status, exclude
White Rabbit is randomly divided into five groups according to random digits table by diet exception person: blank control group (n=8) feeding normal diet
100g, morning and evening each 50g；(+10% yolk powder+84% of+5% lard of 1% cholesterol is common for high in fat group of (N=10) feeding high fat diet
Feed 100g, morning and evening each 50g;Model group (N=10), substance low dose group of the present invention (N=10), substance high dose group of the present invention
(N=10), positive controls (Simvastatin group) (N=10) are the same as high in fat group of nursing.Model group, substance low dose group of the present invention, sheet
Invention substance high dose group, positive controls carry out right carotid artery balloon injured art, and blank control group carries out sham-operation simultaneously,
The weighing of 4 weekends takes auricular vein measurement blood lipid level and serum levels of inflammatory cytokines, in addition to blank control group and model group, other components
It does not give: being 0.5g/ days according to tested material human body recommended amounts, press adult's 60kg batheroom scale, be equivalent to daily per kilogram of body weight and push away
Recommending low dosage is 8.33mg, and intragastric administration on mice amount is 30 times that human body recommends low dosage 8.33mg/Kg daily, then substance of the present invention is low
Dosage group 0.25g/kg, substance high dose group 1.5g/kg of the present invention, positive controls (Simvastatin) 5mg/kg continue nursing 8
Week, the weighing of 12 weekends take auricular vein measurement blood lipid level and serum levels of inflammatory cytokines.Dead animal is removed during research in time.
2.2, modeling: experimental rabbit can't help water in preoperative 12h fasting, with 3% yellow Jackets (0.5-1ml/kg) Jing Eryuan
Intravenous Anesthesia, after anaesthetizing steadily, fixation of facing upward, neck preserved skin, routine disinfection drape, row neck median incision, length about 4-
5cm is exposed to thyroid cartilage upper horizontal, and free right common carotid artery (CCA), right internal carotid artery (ICA) and right neck respectively
Outer artery (ECA) is ligatured in ECA distal end, distance ICA, ECA bifurcated about 2cm using silk thread, artery clamp temporarily press from both sides close ICA and
CCA lifts ECA ligature, and using eye scissors, downstream cuts the notch of about 1mm at ECA ligation, is sent into sacculus, injection life
It manages in salt water to sacculus, maintains 4atm pressure, unclamp artery clamp at CCA, feeding sacculus to 10cm or so, increase ball intraluminal pressure
To 8-12atm, sacculus of slowly pulling back is to clip, and liquid keeps 4atm pressure in pumpback sacculus, and sacculus is sent to depths again,
Repeat aforesaid operations 3 times, exit sacculus, ligature ECA clip downstream, unclamp ICA and CCA and locate artery clamp, observation vascular pulsation and
Wound situation, if any blutpunkte hemostasis by ligation.Physiological saline, ANER DIAN clean visual area, and it is single that leg muscle injects gentamicin 40,000
Position prevention infection, layer-by-layer suture notch.Blank control group row sham-operation, is not inserted into sacculus, remaining step is same as above.
2.3, Testing index: 4 weekend of test experience animal feeding of blood lipid, inflammation index, auricular vein acquire venous blood
4ml, blood sample stands 30min, and after pharmaceutical intervention 8 weeks, that is, before putting to death, auricular vein acquires venous blood 4ml again, and blood sample is stood
30min is detected using Selectra-E full automatic biochemical apparatus.Using ELISA method detection interleukin-6 (IL-6),
Monocyte chemoattractant protein -1 (MCP-1) and Peroxisome proliferater-activated receptor γ1 (PPAR γ).
2.4, statistical method: experimental data carries out statistical analysis with SPSS19.0 statistical software, and measurement data is with equal
Number ± standard deviation (x ± s) indicates that mean compares using one-way analysis of variance between multiple groups, compares before and after same study subject and adopts
It is that difference is statistically significant with P < 0.05 with paired t-test.
3, analysis of experimental results
Raising 4 weeks, 12 weekend blood serum IL-6s, the horizontal comparison result of MCP-1 are shown in Fig. 1.
Note: a be p<0.05, contemporaneity with normal group compared with, b be p<0.05, contemporaneity compared with model group, c p>
0.05, for contemporaneity compared with positive controls, d is the contemporaneity of p < 0.05 compared with 4 weekend levels.
As shown in Figure 1, model group IL-6, MCP-1 level 12 weeks, positive controls significantly raised (p < 0.05) compared with 4 weekends,
Substance low dose group of the present invention and substance high dose group of the present invention are obvious compared with 4 weekend of model group IL-6, MCP-1 level and 12 weekends
It reduces (p < 0.05), substance high dose group of the present invention reduction degree compared with positive controls becomes apparent from (p < 0.05).
Embodiment 2
Using 100kg virgin rubber tree species as raw material, after drying shelling, kernel 40kg is prepared.Kernel material arrives 80- in heat
100 DEG C, then pass through dynamic screw extrusion, the screw extrusion time is 45 seconds or so, and squeezing out 15kg liquid is this hair
Bright substance seeds of rubber tree fatty contents.
Using the lipoclastic of 10kg seeds of rubber tree as raw material, 10kg potassium hydroxide-ethanol solution is added and carries out saponification,
The mass percent concentration of potassium hydroxide in ethanol is 22%.After saponification terminates, under 0.005atm vacuum condition, add
Heat is to 120 DEG C of recycling ethyl alcohol；5kg ethyl acetate is added later to extract as unsaponifiable matter extractant, isolates upper layer of extraction liquid,
Under 0.01atm vacuum condition, 120 DEG C of recycling extractants are heated to, it is of the invention for obtaining the remaining liquid of 440g
The unsaponifiable matter of the fatty contents of seeds of rubber tree.
1, experimental animal: healthy beasle dog 20, male, weight is between 15-20kg, by Chinese Academy of Sciences Kunming animal
Research institute provides.
2, experimental method
2.1, it is grouped: beasle dog random number being grouped, sham-operation A group, sham-operation B group, low dose of substance of the present invention are divided into
Amount group, substance high dose group of the present invention, totally 4 groups, every group 5.In Kunming, zooscopy institute is fed during experiment, every ratio
Lattice dog is housed individually, and daily ration is the 4% of weight, normal water, high lipid food preparation: basal feed 84.0%；Butter 6.0%；
Peanut oil 6.0%；Bovine bile 1.0%；Cholesterol 3%.All experiment beasle dogs are in preoperative 24 hours of modeling and postoperative 3
Its feeding aspirin enteric coated tablet 300mg/d, is changed to aspirin enteric coated tablet 100mg/d later, until experimental endpoints.It is making
It is anticoagulant that heparin sodium is given in mold process.
It is 0.5g/ days according to tested material human body recommended amounts, presses adult's 60kg batheroom scale, be equivalent to daily per kilogram of body weight and push away
Recommending low dosage is 8.33mg, and feed intake is the 1/5 of its weight, then substance low dose group additive amount of the present invention is 1.25g/kg(phase
When in 30 times of human body recommended intake low dosage), substance high dose group additive amount of the present invention is same as above for 7.5g/kg(), artificial hand
Art B group is added substance 7.5g/kg of the present invention at the very start and is fed together, and sham-operation A group only carries out high lipid food nursing, until real
Test terminal.
2.2, modeling: substance high dose group of the present invention and substance low dose group of the present invention carry out right carotid artery balloon injured
Art carries out modeling.Sham-operation group modeling: Animal Anesthesia process is identical as stenosis models group.Modus operandi is as follows: firstly, disinfection
Operative region；Secondly, exposure, free right carotid；Finally, layer-by-layer suture wound immediately after right carotid is free.
After operation, beasle dog is sent into independent stable breeding in feeding room；Give within postoperative 3 days 1,600,000 u muscle of penicillin note
1 time/d and disinfection wound are penetrated to prevent to infect；Animal situation, substance high dose group of the present invention, object of the present invention are observed and recorded daily
After matter low dose group gives High-fat diet 2 months, color ultrasound examination is carried out respectively.Substance height dosage group of the present invention carries out respectively
Gastric infusion continues stomach-filling 4 weeks, chow diet nursing, normal water is carried out during stomach-filling.Sham-operation A, B group feed always 3
Month.Color ultrasound examination is carried out after nursing again.
3, experimental result and analysis
The experiment of table 1 front and back color ultrasound examines patch situation
| Group | n | Whether there is or not patches before experiment | Whether there is or not patches after modeling | Whether patch improves after March |
| Sham-operation A group | 5 | Nothing | Have | It is no |
| Sham-operation B group | 5 | Nothing | Have | It is |
| Substance low dose group of the present invention | 5 | Nothing | Have | It is |
| Substance high dose group of the present invention | 5 | Nothing | Have | It is |
The situation of change result of experiment front and back each group IMT value is shown in Fig. 2.
Note: * indicates p < 0.05 compared with sham-operation A group；* indicates that different times compare p < 0.05 with group.
As shown in Table 1, patch is formed in each group arteria carotis after modeling success, substance of the present invention is high after administration 4 weeks
Low dose group and sham-operation B group patch have clear improvement.As shown in Figure 2, sham-operation B group 2 months when IMT value and at 3 months
IMT value there were significant differences compared with sham-operation A group p < 0.05, illustrates that substance of the present invention has certain prevention of arterial atherosis
Effect；It is anisotropic that substance low dose group different times of the present invention compare no significance difference, after substance high dose group modeling of the present invention with give
Comparing IMT value after medicine significantly reduces p < 0.05, illustrates that substance of the present invention has the effect for the treatment of atherosclerosis.
Embodiment 3
Using 100kg virgin rubber tree species as raw material, after drying shelling, kernel 40kg is prepared.Then kernel is subjected to extruding
After processing, it is put into the leaching container of sealing, the solvent of 2 times of weight is added.In 55 DEG C of (being lower than solvent boiling point) conditions and normal pressure
Under, material is stirred and is mixed with solvent, material is allowed to come into full contact with solvent.It staticly settles later, leaches solvent, then
120 DEG C (being higher than solvent boiling point temperature) under 100Pa vacuum condition, solvent is allowed to volatilize, staying 18kg liquid is
Substance seeds of rubber tree fatty contents of the invention.
Using the lipoclastic of 10kg seeds of rubber tree as raw material, 10kg potassium hydroxide-ethanol solution is added and carries out saponification,
The mass percent concentration of potassium hydroxide in ethanol is 22%.After saponification terminates, under 0.005atm vacuum condition, add
Heat is to 120 DEG C of recycling ethyl alcohol；N-hexane is added later to extract as unsaponifiable matter extractant, isolates upper layer of extraction liquid,
Under 0.005atm vacuum condition, 120 DEG C of recycling extractants are heated to, obtaining 430g liquid is rubber tree species of the invention
The unsaponifiable matter of the fatty contents of son.
1, experimental animal: SPF grades of healthy rats are provided, production licence number by Kunming Institute of Zoology, Chinese Academy of Sciences:
The Yunnan SCXK() K2016-0005.40, every group 8, divide 5 groups, 200 ~ 220g of weight.
2, experimental method
2.1, modeling and grouping: experimental rat feeding high lipid food (3% cholesterol, 0.5% sodium taurocholate, 0.2% propylthiouracil,
5% white sugar, 10% lard, 81.3% basal feed) daily 100g, each 50g, feeds 4 weeks sooner or later.It is initially simultaneously primary in feeding
Property intraperitoneal injection 3 60 ten thousand IU/kg of vitamin D, construct As rat model.4 weekends by As rat model be grouped at random stomach-filling to
Medicine.It is 0.5g/ days according to tested material human body recommended amounts, presses adult's 60kg batheroom scale, be equivalent to daily per kilogram of body weight recommended amounts
For 8.33mg, substance stomach-filling amount of the present invention is 30 times of human body recommended intake, then substance low dose group administration of the present invention
1.5g/kg, A substance (linseed oil) high dose (40mg/kg) BW, B substance is administered in 0.25g/kg, the advanced amount group of substance of the present invention
(perilla herb oil) high dose (40mg/kg) BW, C substance (maize germ oil) high dose (40mg/kg) BW.
2.2, Testing index: continuous gavage 40 days, TG, HDL-C, TC is carried out to As rat model respectively later and detected
Analysis, assesses anti-As effect.
2.3, statistical analysis: data analysis is carried out using SPSS19.0 software, is examined using t, p < 0.05 has statistics
Learn meaning.
3, experimental result and analysis
Result of variations is shown in Fig. 3 before and after each group serum TC, TG, HDL-C concentration experiment.
From the figure 3, it may be seen that there was no significant difference (P > 0.05) and high dose ABC for the detected value of the preceding groups of animals serum TC of experiment
Substance group is compared, and substance of the present invention is low, high dose group has reduction to Serum TC, there were significant differences for high dose group (P <
0.05).The detected value of groups of animals serum TG is without significant difference before testing, and compared with other groups, substance of the present invention is low, high agent
Amount group has reduction to rat blood serum TG, and there were significant differences for high dose group (P < 0.05).Groups of animals Serum HDL-C before testing
Detected value is without significant difference, and substance of the present invention is low compared with other groups, high dose group has raising to rat blood serum HDL-C,
There were significant differences for high dose group (P < 0.05).The result shows that the unsaponifiable matter of seeds of rubber tree fatty contents can effectively drop
Low rat fat, triglyceride and rat high-density lipoprotein cholesterol is effectively improved, can effectively prevent and treat artery
Atherosis.
Embodiment 4
Using 100kg virgin rubber tree species as raw material, after drying shelling, kernel 40kg is prepared.Kernel is subjected to granulation processing
Later, it is put into the supercritical extract container of sealing pressure resistance, solvent is added, solvent quality is 2 times of quality of material.At 35 DEG C,
10MPa(is more than the critical-temperature and critical pressure of solvent) under conditions of, material is stirred and is mixed with solvent, allows material
It is come into full contact with solvent.It staticly settles later, leaches solvent, then under room temperature at 25 DEG C, reduce pressure to normal pressure
(0.1MPa), allows solvent to volatilize, and staying 18kg liquid is substance seeds of rubber tree fatty contents of the invention.
Using the lipoclastic of 10kg seeds of rubber tree as raw material, 10kg potassium hydroxide-ethanol solution is added and carries out saponification,
The mass percent concentration of potassium hydroxide in ethanol is 22%.After saponification terminates, under 0.005atm vacuum condition, add
Heat is to 120 DEG C of recycling ethyl alcohol；5kg6 solvent is added later to extract as unsaponifiable matter extractant, isolates upper layer of extraction liquid,
Under 0.005atm vacuum condition, 140 DEG C of recycling extractants are heated to, it is of the invention for obtaining the remaining liquid of 450g
The unsaponifiable matter of the fatty contents of seeds of rubber tree.
Now cooperate with certain hospital, extracts 100 Carotid Artery Atherosclerotic Patients (CAS) (degree of carotid atherosclerosis
The degree of systemic atherosclerosis can directly be reacted), 100 patients are randomly divided into two groups, substance group of the present invention and control
Group (unsaponifiable matter of the fatty contents of Purple Perilla Seed Oil), takes 12 months, carries out double blind experiment.Age and gender are not to influence
The influence factor of CAS is first detected before and after experiment respectively using Color Doppler ultrasound, and record is suffered from any time during the experiment
Person's physical condition then stops experiment if any uncomfortable immediately.
Color Doppler ultrasonography arteria carotis can clearly show canalis caroticus wall construction, clearly show that the narrow journey of lumen
Degree detects patch and thrombus, identifies soft or hard spot, is capable of the change of accurate judgement Carotid Sclerosis, provides strength artery sclerosis
Thickness, feature and location information etc., and reproducible, non-invasive are that a kind of expense is low, the significant hurtless measure of diagnostic value
Property diagnostic method.Taking substance group dose of the present invention is 0.5g/ days, and the unsaponifiable matter of the fatty contents of Purple Perilla Seed Oil is taken
The improvement situation of the case where amount is 0.5g/ days, observes carotid plaques and Carotid Intima-media Thickness (IMT).
Two groups of IMT measured value comparison results are shown in Fig. 4.
Note: it is indicated when IMT < 1.0mm normal；IMT indicates intimal thickening between 1.0-1.2mm；IMT is in 1.2-1.4mm
Between indicate that patch is formed；IMT > 1.4mm indicates carotid artery stenosis.
As shown in Figure 4, it indicates that patch is formed between 1.2-1.4mm in case group IMT value each before experiment, is allusion quotation
The atherosclerotic of type.IMT value is significantly improved compared with the control group after taking substance of the present invention.And in the phase of taking
Between without discovery have other malaise symptoms generation.It takes substance group patient of the present invention and obviously feels that body becomes to become better and better.
Embodiment 5:
The unsaponifiable matter of seeds of rubber tree fatty contents of the invention is made by the method in embodiment 1-4, by the part with
Excipient, pelletizing press sheet is added in the ratio of excipient weight ratio 1:1 or 1:2.Tablet can be used as drug, health food or food
Product.
Embodiment 6:
The unsaponifiable matter of seeds of rubber tree fatty contents of the invention is made by the method in embodiment 1-4, by the part, packet
It is rolled in soft capsule material, routinely soft capsule is made in capsule preparations method.Soft capsule can be used as drug, health food or
Food.
Embodiment 7:
Added by the unsaponifiable matter that seeds of rubber tree fatty contents of the invention are made in the method in embodiment 1-4 with the part
Upper emulsifier, osmotic pressure regulator and water obtain emulsion for injection by high-pressure homogeneous emulsification.The emulsion for injection can be made
For drug.
Embodiment 8:
The unsaponifiable matter of seeds of rubber tree fatty contents of the invention is made by the method in embodiment 1-4, by the part, then
Tablet is made as follows:
Tablet: the unsaponifiable matter 100mg of seeds of rubber tree fatty contents
Appropriate starch
Appropriate corn pulp
Magnesium Stearate proper quantity
Preparation method: the unsaponifiable matter of seeds of rubber tree fatty contents is mixed with auxiliary agent, sieving, in suitable container
Even mixing, obtained granulating mixture tabletting.
Embodiment 9:
The unsaponifiable matter of seeds of rubber tree fatty contents of the invention is made by the method in embodiment 1-4, by the part, then
Soft capsule is made as follows:
Soft capsule: the unsaponifiable matter 500mg of seeds of rubber tree fatty contents
Appropriate gelatin
Appropriate glycerol
Appropriate amount of water
Preparation method: gelatin, glycerol, which are put into distilled water, to impregnate makes gelatin expansion, softening, is then uniformly mixed, obtains
Capsule material glue.Prepared capsule material glue is taken out, is coated in flat plate surface, keeps thickness uniform, then with 90 DEG C or so
Temperature heating, evaporates surface moisture, becomes the cushion compound for having certain toughness, having certain elasticity.Finally with pelleting mold or
Automatic rotation rolling capsule machine, the unsaponifiable matter (500mg) of seeds of rubber tree fatty contents is fitted into soft capsule skin, is produced soft
Capsule.
Embodiment 10:
The unsaponifiable matter of seeds of rubber tree fatty contents of the invention is made by the method in embodiment 1-4, by the part, then
Emulsion for injection is made as follows:
Emulsion for injection: the unsaponifiable matter 200g of seeds of rubber tree fatty contents
Appropriate lecithin
Appropriate glycerol
Appropriate sodium chloride
Appropriate amount of water
Preparation method: according to formula, by the unsaponifiable matter of seeds of rubber tree fatty contents, lecithin, glycerol mixing is added water to
1000mL, high-pressure homogeneous emulsification, 121 DEG C of sterilizings are down to room temperature to get emulsion for injection is arrived.
Embodiment 11:
Tablet: the unsaponifiable matter 10mg of seeds of rubber tree fatty contents of the invention being made by the method in embodiment 1-4, cream
Sugared 180mg, starch 55mg, magnesium stearate 5mg；
Preparation method: by be made by the method in embodiment 1-4 seeds of rubber tree fatty contents of the invention unsaponifiable matter,
Newborn sugar and starch mixing, is uniformly moistened with water, and the mixture after moistening is sieved and is dried, re-sieving, magnesium stearate is added, so
Afterwards by mixture tabletting, every slice weight 250mg, the unsaponifiable matter content of seeds of rubber tree fatty contents of the invention is 10mg.
Embodiment 12:
Capsule: the unsaponifiable matter of seeds of rubber tree fatty contents of the invention is made by the method in embodiment 1-4
2000g, gelatin 1000g, glycerol 500g, water 1000g.
Preparation method: by gelatin 1000g, glycerol 500g, being put into 1000g water to impregnate makes gelatin expansion, softening, then stirs
It mixes uniformly mixed, obtains capsule material glue.Prepared capsule material glue is taken out, is coated in flat plate surface, keeps thickness uniform, so
It is heated afterwards with 90 DEG C or so of temperature, evaporates surface moisture, become the cushion compound for having certain toughness, having certain elasticity.Finally
With pelleting mold or automatically rolling capsule machine is rotated, by the unsaponifiable matter 500mg of the unsaponifiable matter of seeds of rubber tree fatty contents
It is fitted into soft capsule skin, soft capsule is made.Every soft capsule weight 800mg, seeds of rubber tree fatty contents of the invention are not
Saponifiable content is 500mg.
Embodiment 13:
Emulsion for injection: the unsaponifiable matter of seeds of rubber tree fatty contents of the invention is made by the method in embodiment 1-4
200g, glycerol 25g, lecithin 12g, water 1000g, sodium chloride 1g.
Preparation method: seeds of rubber tree fatty contents of the invention are made not by the method in embodiment 2-5 in accurate weighing
Saponified 200g, glycerol 25g, lecithin 12g, mixing, add water to 1000mL, add sodium chloride, by osmotic pressure adjust to
350mosm/kg H2O.High-pressure homogeneous emulsification obtains emulsion, and 121 DEG C of sterilizings are down to room temperature, are aseptically filled to
It is encapsulated in the sterile glass vials of 250mL.Every bottle of emulsion for injection specification is 250mL, seeds of rubber tree fatty contents of the invention
Unsaponifiable matter content be 50g.
Embodiment 14:
Food: the unsaponifiable matter of seeds of rubber tree fatty contents of the invention is made by the method in embodiment 1-4, by the portion
Point, it is reconciled with oil with common edible, is directly becoming food (edible oil).
Preparation method: the not soap of seeds of rubber tree fatty contents of the invention is made by the method in embodiment 1-4 by 1kg
Compound is added in 9kg rapeseed oil, obtains 10kg blending edible oil.By the filling vial at 500g of blending edible oil, every bottle
The unsaponifiable matter content of seeds of rubber tree fatty contents of the invention is 50g in oil.
Claims (10)
1. the unsaponifiable matter of seeds of rubber tree fatty contents, it is characterised in that the seeds of rubber tree fatty contents are not saponified
Object is prepared by the following method and obtains:
Using virgin rubber tree species as raw material, after drying shelling, kernel is prepared, kernel passes through mechanical presses method, extraction
The lipoclastic of seeds of rubber tree is made in one or more of method, supercritical extraction, then with the lipoclastic of seeds of rubber tree
For raw material, excessive potassium hydroxide-ethanol solution is added and carries out saponification, after saponification terminates, under vacuum conditions,
Heating recovery ethyl alcohol, later be added unsaponifiable matter extractant extracted, selected extractant be ether, ethyl acetate, n-hexane,
One of No. 6 solvents isolate upper layer of extraction liquid, under vacuum conditions reheating, recycle extractant, remaining liquid object
Matter is the unsaponifiable matter of the fatty contents of seeds of rubber tree.
2. the unsaponifiable matter of the seeds of rubber tree fatty contents described in claim 1 containing therapeutically effective amount and pharmaceutically may be used
The pharmaceutical composition of the carrier of receiving.
3. health food is commonly used by the unsaponifiable matter and health food of seeds of rubber tree fatty contents described in claim 1
Carrier is formed.
4. food, by the unsaponifiable matter and food common carrier institute group of seeds of rubber tree fatty contents described in claim 1
At.
5. the unsaponifiable matter of seeds of rubber tree fatty contents described in claim 1 prevents and treats Atherosclerosis in preparation
Application in the drug of change.
6. application as claimed in claim 5, which is characterized in that the unsaponifiable matter of the seeds of rubber tree fatty contents
As the sole active agent in drug.
7. the unsaponifiable matter of seeds of rubber tree fatty contents described in claim 1 prevents and treats Atherosclerosis in preparation
Application in the health food of change.
8. application as claimed in claim 7, which is characterized in that the unsaponifiable matter of the seeds of rubber tree fatty contents
As sole active agent.
9. the unsaponifiable matter of seeds of rubber tree fatty contents described in claim 1 prevents and treats Atherosclerosis in preparation
Application in the food of change.
10. application as claimed in claim 9, which is characterized in that the seeds of rubber tree fatty contents are not saponified
Object is as sole active agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711140838.0A CN109793803A (en) | 2017-11-16 | 2017-11-16 | A kind of application and preparation method of the substance preventing and treating atherosclerosis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711140838.0A CN109793803A (en) | 2017-11-16 | 2017-11-16 | A kind of application and preparation method of the substance preventing and treating atherosclerosis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109793803A true CN109793803A (en) | 2019-05-24 |
Family
ID=66555815
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711140838.0A Pending CN109793803A (en) | 2017-11-16 | 2017-11-16 | A kind of application and preparation method of the substance preventing and treating atherosclerosis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109793803A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1154217A (en) * | 1996-12-11 | 1997-07-16 | 曹敏 | Method for making edible health-care kernel and edible oil from seed of rubber tree |
| CN1215059A (en) * | 1997-10-18 | 1999-04-28 | 李世泰 | Extration of steroid compound from rubber seeds |
-
2017
- 2017-11-16 CN CN201711140838.0A patent/CN109793803A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1154217A (en) * | 1996-12-11 | 1997-07-16 | 曹敏 | Method for making edible health-care kernel and edible oil from seed of rubber tree |
| CN1215059A (en) * | 1997-10-18 | 1999-04-28 | 李世泰 | Extration of steroid compound from rubber seeds |
Non-Patent Citations (3)
| Title |
|---|
| 李世泰，等: "橡胶种子活性物质探讨", 《热带作物学报》 * |
| 汪勇: "《粮油副产物加工技术》", 31 July 2016, 暨南大学出版社 * |
| 王慧敏: "橡胶籽油的提取、精炼及其氧化稳定性研究", 《中国优秀硕士学位论文全文数据库 工程科技Ι辑》 * |
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Application publication date: 20190524 |