CN109790504A - 一种从组织分离细胞的装置 - Google Patents

一种从组织分离细胞的装置 Download PDF

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Publication number
CN109790504A
CN109790504A CN201680089583.7A CN201680089583A CN109790504A CN 109790504 A CN109790504 A CN 109790504A CN 201680089583 A CN201680089583 A CN 201680089583A CN 109790504 A CN109790504 A CN 109790504A
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cups
internal layer
tissue
blade
cell
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林哲信
王耀贤
陈崇桓
张瑞根
何美泠
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Kaohsiung Medical University
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Kaohsiung Medical University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/02—Blood transfusion apparatus
    • A61M1/029—Separating blood components present in distinct layers in a container, not otherwise provided for
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/09—Means for pre-treatment of biological substances by enzymatic treatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G01N2001/2873—Cutting or cleaving

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Cell Biology (AREA)
  • Mechanical Engineering (AREA)
  • Hematology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Anesthesiology (AREA)
  • Vascular Medicine (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

本发明提供了一种将细胞从组织中快速分离的装置，包括：一搅拌剪切构件，具有一旋转式剪切刀具，由马达驱动旋转对组织进行搅拌与切割，并可加入消化酵素以加速分解所述组织；以及一内外双层套杯，用于容纳过滤出具有所要细胞的溶液，并由所述内杯的过滤装置筛出所需的细胞，滤出的溶液由外杯收集。

Description

PCT国内申请，说明书已公开。

Claims (11)

  1. PCT国内申请，权利要求书已公开。
CN201680089583.7A 2016-09-29 2016-09-29 一种从组织分离细胞的装置 Pending CN109790504A (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2016/100816 WO2018058435A1 (zh) 2016-09-29 2016-09-29 一种从组织分离细胞的装置

Publications (1)

Publication Number Publication Date
CN109790504A true CN109790504A (zh) 2019-05-21

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Country Link
US (1) US20200033232A1 (zh)
EP (1) EP3521413A4 (zh)
JP (1) JP2019528728A (zh)
KR (1) KR20190042688A (zh)
CN (1) CN109790504A (zh)
TW (1) TWI635174B (zh)
WO (1) WO2018058435A1 (zh)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110919706A (zh) * 2019-11-01 2020-03-27 上海蓓蕊医疗科技有限公司 一种细胞精密切割装置及其使用方法
CN114144207A (zh) * 2019-05-06 2022-03-04 特苏米尔科技有限责任公司 无创伤地形成的组织组合物、装置、制备方法和治疗方法
CN114958720A (zh) * 2022-06-07 2022-08-30 王翠玲 一种细胞分离方法

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CN110484435A (zh) * 2019-08-22 2019-11-22 广州市尤德生物科技有限公司 一种制备单细胞悬液的组织分离器
CN111607508B (zh) * 2020-04-28 2023-03-10 深圳大学 一种自动型间充质干细胞分离装置
CN111735675B (zh) * 2020-06-05 2023-09-29 塔里木大学 一种动物组织样品预处理分离装置
US11987787B2 (en) 2020-07-22 2024-05-21 AVITA Medical Americas, LLC Devices, methods, and kits for preparing a cell suspension
GB2597767A (en) * 2020-08-04 2022-02-09 Achilles Therapeutics Uk Ltd Disaggregation device
CN112126590A (zh) * 2020-10-28 2020-12-25 北京中源合聚生物科技有限公司 一种细胞贴壁微载体接种装置及接种方法
CN112500995A (zh) * 2020-12-22 2021-03-16 北京金莎科技有限公司 一种自体细胞微架构疗法装置
KR102507420B1 (ko) * 2021-10-05 2023-03-07 의료법인 명지의료재단 세포배양용 조직 분쇄 및 효소 반응기
KR102640156B1 (ko) * 2021-10-20 2024-02-27 주식회사 로킷헬스케어 슬개하 지방패드 분쇄장치
WO2023181636A1 (ja) * 2022-03-23 2023-09-28 富士フイルム株式会社 細胞単離方法及び細胞単離装置
CN118028087B (zh) * 2024-04-15 2024-07-19 丽江山荷生物科技有限公司 一种鸡豆中纳豆激酶的分离设备及其分离方法

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US4028190A (en) * 1974-11-07 1977-06-07 Merck & Co., Inc. Apparatus for preparing isolated cells from tissue
CN1919431A (zh) * 2005-04-29 2007-02-28 艾卡工厂有限及两合公司 搅拌或分散装置
EP1994910A1 (en) * 2006-02-24 2008-11-26 Health Sciences Univ. of Hokkaido Milled product of extracted tooth usable in highly advanced medical treatment, decalcified powder originating in extracted tooth, method of preparing composite of decalcified powder with apatite and milling machine
CN101096699A (zh) * 2006-06-27 2008-01-02 米利波尔公司 用于制备液体微生物分析样品的方法和装置
CN104997730A (zh) * 2009-12-03 2015-10-28 诺华股份有限公司 就微流化排列交互作用和背压室
CN201675769U (zh) * 2010-04-29 2010-12-22 王晓东 食品处理机交互剪切式粉碎刀具结构
US20110282238A1 (en) * 2010-05-13 2011-11-17 Houser Kevin L Method and Apparatus for Morcellating Tissue
CN102382757A (zh) * 2010-08-27 2012-03-21 日本光电工业株式会社 细胞离析装置
CN102899241A (zh) * 2011-07-26 2013-01-30 日本光电工业株式会社 细胞分离仪器
US8967513B1 (en) * 2011-11-08 2015-03-03 Auxocell Laboratories, Inc. Systems and methods for processing cells
CN103070718A (zh) * 2012-12-31 2013-05-01 陈金西 皮肤组织细胞分离机
CN103469309A (zh) * 2013-09-12 2013-12-25 博雅干细胞科技有限公司 一种组织匀浆法分离活细胞构建细胞库的方法
CN203639459U (zh) * 2013-09-12 2014-06-11 博雅干细胞科技有限公司 一种分离活细胞的组织处理器
CN203815262U (zh) * 2014-03-19 2014-09-10 九阳股份有限公司 高效粉碎的豆浆机
WO2015161057A1 (en) * 2014-04-18 2015-10-22 Auxocell Laboratories, Inc. Systems and methods for processing cells
CN204583176U (zh) * 2015-02-12 2015-08-26 安徽安成工业设备有限公司 一种粉碎功能的反应釜搅拌装置
CN204752692U (zh) * 2015-06-26 2015-11-11 哈尔滨壹加壹再生医学科技有限公司 用于分离脂肪干细胞的装置
CN205420437U (zh) * 2015-12-23 2016-08-03 天津滨通生物科技有限公司 脂肪干细胞分离提取装置
CN205587088U (zh) * 2016-03-24 2016-09-21 张炳煌 一种环保型医药制备用粉碎搅拌机
CN205340947U (zh) * 2016-04-18 2016-06-29 江苏希瑞干细胞技术有限公司 胎盘组织切割装置
CN105875094A (zh) * 2016-05-20 2016-08-24 太仓市雅丰农场专业合作社 一种水生植物粉碎装置

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114144207A (zh) * 2019-05-06 2022-03-04 特苏米尔科技有限责任公司 无创伤地形成的组织组合物、装置、制备方法和治疗方法
CN114144207B (zh) * 2019-05-06 2022-10-14 特苏米尔科技有限责任公司 无创伤地形成的组织组合物、装置、制备方法和治疗方法
CN110919706A (zh) * 2019-11-01 2020-03-27 上海蓓蕊医疗科技有限公司 一种细胞精密切割装置及其使用方法
CN114958720A (zh) * 2022-06-07 2022-08-30 王翠玲 一种细胞分离方法

Also Published As

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US20200033232A1 (en) 2020-01-30
TW201812000A (zh) 2018-04-01
EP3521413A4 (en) 2020-06-17
KR20190042688A (ko) 2019-04-24
JP2019528728A (ja) 2019-10-17
EP3521413A1 (en) 2019-08-07
WO2018058435A1 (zh) 2018-04-05
TWI635174B (zh) 2018-09-11

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Application publication date: 20190521