A kind of antibacterial fabric and preparation method thereof
Technical field
The present invention relates to a kind of antibacterial fabrics and preparation method thereof.
Background technique
With the increasingly raising of living standard, people increasingly pay attention to health, when selecting clothing in addition to nationwide examination for graduation qualification
Consider outside its color and style, can also go to consider its health.Fiber is big by its elasticity modulus, and plastic deformation is small, intensity height etc.
Feature plays an increasingly important role in people's lives, especially the dress of people, as clothes, socks etc be even more from
Fibrillation is not tieed up.But fiber product is because the chemical structure of its porous type body form and high molecular polymer is attached conducive to microorganism
, so that such fiber product is become the good parasitic body of microbe survival, breeding, this will cause certain harm to human body.?
Play the role of Some Enterprises the nano silver with antimicrobial component be added in fabric to reach antibacterial, but such method cost compared with
Height is unfavorable for the production of medium-sized and small enterprises.
A kind of high tenacity antibacterial fabric, including the first antibiotic layer, the first articulamentum, first are disclosed in CN205658418U
Tough silk layer, mixing ductile layers, the second antibiotic layer, the second articulamentum and the second tough silk layer, first antibiotic layer and the first tough silk
It is fixedly connected with the first articulamentum between layer, the first tough silk layer is fixedly connected with mixing ductile layers, mixing ductile layers side
It is fixedly connected with the second tough silk layer, second tough silk layer side is fixedly connected with the second articulamentum, and second articulamentum side is solid
Surely the second antibiotic layer is connected.This kind of high tenacity antibacterial fabric, passes through the first antibiotic layer and the second antibiotic layer.But the antibacterial fabric
In specific antibiotic layer disclose be what material composition, how antibacterial effect is also without disclosing.
A kind of preparation method of antibacterial blended yarn weaved fabric is also disclosed in CN105040241A, first by 50% cotton/30% of warp thread
Flax/20% bamboo fibre * weft yarn, 100% cotton interweave to be formed blended yarn weaved fabric grafting one chloro-s-triazine-beta-cyclodextrin, then by one
Blended yarn weaved fabric after chloro-s-triazine-grafted by beta cyclodextrin is put into the silver nitrate solution dipping 60min of 0.01-10g/L, two leachings two
It rolls, being put into heating 8min in the micro-wave oven of 600W can be obtained antibacterial blended yarn weaved fabric.But this method is also without being related to antibacterial
The description of effect, and preparation method will also use micro-wave oven and be not suitable for large-scale promotion application.
Summary of the invention
In order to overcome the deficiencies in the prior art, the invention mainly solves the technical problem of providing a kind of sides of preparation
Method is easy, and can significantly improve the ability of the antibacterial of clothing, moreover it is possible to solve the problems, such as gas permeability, while can also improve clothing
Antibiotic property and mosquito-proof ability.
In order to solve the above technical problems, one technical scheme adopted by the invention is that:
A kind of antibacterial fabric, each component including following weight parts: 60 parts of Lyocell fibers, 40 parts of flaxen fibers, 15 portions of sheep
Wool fibre, 5 parts of chitin fibers, 10 parts of polyester filament fibers, 0.01 part of antibacterial peptide;After antibacterial fabric is handled using antibacterial peptide
It is blended and weave.
Wherein, blended and weaving process is method generally in the art, for example, blended process flow are as follows: pretreatment-is clear
Cotton-cotton carding-drafting-rove-spun yarn-winder.Weaving process process are as follows: warping-sizing-drawing-in-weaving.
The antibacterial peptide processing step is to impregnate in antibacterial peptide solution;
The antibiotic finishing solution is that antibacterial peptide is added in water, adjusts the pH value of antimicrobial fluid to 6.5;
The sequence of the antibacterial peptide is SEQ ID NO:1:HPQMVNRPFYQTRKRFDSCPEAHPARI or SEQ ID
NO:2:KHQRIEAPSRHYWKRYRMCHDYWQSTK;Or SEQ ID NO:3:RPPLTQDYHMPPQMRLLRWKKDTWAWV;
Or SEQ ID NO:4:YMQDWPKEMLPHFPNWANQCCWSQPPF;Or SEQ ID NO:5:
HIQEQEPYMYISARKARFMMHSFHWKP。
It is further preferred that the step of antibacterial peptide is handled are as follows: after being impregnated 50 minutes in antibacterial peptide solution under room temperature
It takes out, adds and carry out crosslinking fixation containing 60 degrees Celsius in 5% chitin solution, so that antibacterial peptide is capable of fixing anti-
On bacterium fabric, long-term antibacterial effect is maintained.
The present invention further provides a kind of preparation method of antibacterial fabric, it is characterised in that takes 60 kilograms of Lyocell fibres
Dimension, 40 kilograms of flaxen fibers, 15 kilograms of wool fibers, 5 kilograms of chitin fibers, 10 kilograms of polyester filament fibers, shredding is pre- respectively
Processing, is combed into the carded sliver respectively on carding machine;
It on drawing frame and closes, ripe bar is made;
Drawing-off is carried out on fly frame and mixed yarn is made in twisting, the finally further draw twisting on spinning frame;Again will
Mixed yarn is weaved using the bright volume method for weaving of large circle machine, finally obtained antibacterial fabric 60;
Take blended yarn weaved fabric obtained, further progress anti-bacterial finish, specific steps are as follows:
(1) configuration of antiseptic solution:
Weigh 0.01 kilogram of SEQ ID NO:1 or SEQ ID NO:2 or SEQ ID NO:3 or SEQ ID NO:4 or SEQ
The antibacterial peptide of ID NO:5 is dissolved in ultrapure water, is uniformly mixed, and adjusts pH value to 6.5, antibacterial peptide solution is made;
(2) it impregnates:
It takes out, adds molten containing 5% chitin after being impregnated 50 minutes in antibacterial peptide solution under blended yarn weaved fabric room temperature
Crosslinking fixation is carried out for 60 degrees Celsius in liquid, so that antibacterial peptide is capable of fixing on blended yarn weaved fabric, maintains long-term antibacterial effect.
(3) it is dehydrated;
(4) it dries: by fabric drying at a temperature of 60 DEG C.
The present invention further provides a kind of antibacterial fabric that method is prepared according to claim 3.
The present invention further provides a kind of clothes being prepared by the fabric.
The present invention further provides a kind of mask being prepared by the fabric.
The present invention further provides a kind of antibacterial peptide, and sequence is as shown in SEQ ID NO:1.
The present invention further provides a kind of antibacterial peptide, and sequence is as shown in SEQ ID NO:2.
The present invention further provides a kind of antibacterial peptide, and sequence is as shown in SEQ ID NO:3.
The present invention further provides a kind of antibacterial peptide, and sequence is as shown in SEQ ID NO:4.
The present invention further provides a kind of antibacterial peptide, and sequence is as shown in SEQ ID NO:5.
The present invention further provide it is a kind of by the antibacterial peptide in the application prepared in antibacterial fabric.
The beneficial effects of the present invention are:
The pollution that antibacterial peptide enables clothing preferably to prevent and treat mushroom is added, so that clothing is safer.In addition, using
Multiple fiber mixing, improves the gas permeability and thermal diffusivity of clothing, wears more comfortable.
Specific embodiment
The embodiment of the present invention is described in detail below, so that advantages and features of the invention can be easier to by ability
Field technique personnel understanding, so as to make a clearer definition of the protection scope of the present invention.
The preparation of 1 blended yarn weaved fabric 1 of embodiment:
60 kilograms of Lyocell fibers, 40 kilograms of flaxen fibers, 15 kilograms of wool fibers, 5 kilograms of chitin fibers, 10 kilograms
Polyester filament fiber, shredding pretreatment, is combed into the carded sliver respectively on carding machine respectively;
It on drawing frame and closes, ripe bar is made;
Drawing-off is carried out on fly frame and mixed yarn is made in twisting, the finally further draw twisting on spinning frame;Again will
Mixed yarn is weaved using the bright volume method for weaving of large circle machine, and antibacterial fabric (60) of the invention is finally made.
The preparation process of above-mentioned mixed yarn and fabric is the universal method of the industry, is those of ordinary skill in the art
It is known.
Take blended yarn weaved fabric obtained, further progress anti-bacterial finish, specific steps are as follows:
(1) configuration of antiseptic solution:
In 60 parts of Lyocell fibers: the ratio of 0.01 part of antibacterial peptide weighs the antibacterial of 0.01 kilogram of SEQ ID NO:1
Peptide (being obtained using prokaryotic expression system well known in the art purifying) is dissolved in ultrapure water, is uniformly mixed, is adjusted pH value
To 6.5, antibacterial peptide solution is made.
(2) it impregnates:
It takes out, adds molten containing 5% chitin after being impregnated 50 minutes in antibacterial peptide solution under blended yarn weaved fabric room temperature
Crosslinking fixation is carried out for 60 degrees Celsius in liquid, so that antibacterial peptide is capable of fixing on blended yarn weaved fabric, maintains long-term antibacterial effect.
(3) it is dehydrated;
(4) it dries: by fabric drying at a temperature of 60 DEG C.
It can be prepared by antibacterial fabric of the invention.
The preparation of 2 blended yarn weaved fabric 2 of embodiment:
Method and material are with the method and steps of embodiment 1, and wherein antibacterial peptide replaces with the antibacterial peptide of SEQ ID NO:2,
Preparation accordingly obtains corresponding fabric.
The preparation of 3 blended yarn weaved fabric 3 of embodiment:
Method and material are with the method and steps of embodiment 1, and wherein antibacterial peptide replaces with the antibacterial peptide of SEQ ID NO:3,
Preparation accordingly obtains corresponding fabric.
The preparation of 4 blended yarn weaved fabric 4 of embodiment:
Method and material are with the method and steps of embodiment 1, and wherein antibacterial peptide replaces with the antibacterial peptide of SEQ ID NO:4,
Preparation accordingly obtains corresponding fabric.
The preparation of 5 blended yarn weaved fabric 5 of embodiment:
Method and material are with the method and steps of embodiment 1, and wherein antibacterial peptide replaces with the antibacterial peptide of SEQ ID NO:5,
Preparation accordingly obtains corresponding fabric.
The preparation of the control fabric of embodiment 6:
Method and material are with the method and steps of embodiment 1, wherein not adding antibacterial peptide processing, preparation accordingly obtains corresponding
Fabric.
The verifying of 7 antibacterial effect of embodiment
Candida albicans type strain (CICC31284) is purchased from Chinese industrial Microbiological Culture Collection administrative center.
Staphylococcus aureus type strain ATCC 29213, passes through commercially available purchase.
1. the preparation of bacterium solution
It is cultivated using the Candida albicans of this field routine and the culture medium of staphylococcus aureus, continuously transfers two
Candida albicans and S. aureus culture after secondary take a small amount of fresh bacterium from culture medium with oese respectively, add
Enter in sterile saline, using counting method under microscope, being prepared into containing bacterial concentration is 1X 105CFU/ml tests bacterium solution, standby
With.
2. antibiotic rate detects
Antibiotic rate detection is carried out using film cladding process.First by the standby obtained sterile place fabric 1cm*1cm embodiment 1-6
After reason, it is put in culture dish center, takes 20uL experiment staphylococcus aureus bacterium solution and Candida albicans bacterium solution ((1X respectively
105CFU/ml it) is added dropwise in web surface, picks up polyethylene film with sterilizing tweezers and be covered on test specimen, pave, make bacterium solution and examination
Part uniformly contacts.It is aerobic in the case where 35 DEG C of relative humidity are greater than 90% to cultivate for 24 hours.Fabric is taken out later to be put into test tube,
2ml sterile saline is added acutely to shake on the oscillator, after sufficiently shaking up, l0uL is taken to be inoculated in husky Bao Luoshi agar respectively
Plate and MH agar plate at 35 DEG C after aerobic culture for 24 hours, carry out bacterium colony counting, and experiment, which is repeated 3 times, to be averaged.Blank
Control is not add fabric.Calculating antibiotic rate R (%)=(blank control group averagely recycles clump count-antibacterial fabric and averagely recycles
Clump count)/blank control group averagely recycles clump count X 100%.
3. statistical analysis
Statistical analysis, more comparison among groups are carried out using average colony number of the SPSS11.0 statistics software packet to two kinds of bacterium
Using one-way analysis of variance, compares two-by-two between group and examined using LSD-t, there is statistical difference in P < 0.05.Five antibacterial fabrics
It averagely recycles clump count and antibiotic rate the results are shown in Table 1, table 2.
The anti-bacterial result (%) of 1 antibacterial fabric of table to Candida albicans
|
Clump count |
Antibiotic rate |
Blank control |
1490±7.5 |
—— |
Embodiment 1 |
16±1.0 |
98.92 |
Embodiment 2 |
25±2.6 |
98.32 |
Embodiment 3 |
35±5.2 |
98.17 |
Embodiment 4 |
44±6.9 |
98.01 |
Embodiment 5 |
21±8.4 |
98.52 |
Embodiment 6 |
1239±5.9 |
16.85 |
The anti-bacterial result (%) of 2 antibacterial fabric of table to staphylococcus aureus
|
Clump count |
Antibiotic rate |
Blank control |
356±2.3 |
—— |
Embodiment 1 |
8±0.5 |
97.75 |
Embodiment 2 |
11±1.0 |
96.91 |
Embodiment 3 |
20±1.7 |
96.13 |
Embodiment 4 |
18±2.4 |
96.48 |
Embodiment 5 |
31±2.5 |
95.28 |
Embodiment 6 |
324±1.2 |
8.99 |
As can be seen from Table 1 and Table 2, the clump count of the cloth and blank control group that are added to antibacterial peptide carries out statistics
Compare, either to Candida albicans or staphylococcus aureus, result difference is statistically significant (p < 0.05).And
Adding antibacterial peptide has preferable antibacterial effect.
The test of 5 fabric performance of embodiment
Other properties tests are carried out to the antibacterial fabric of embodiment 1-3 preparation below:
It can be seen that the fabric of the invention being prepared with preferable cloth characteristic from general detection data, and from
Embodiment 1-5 result, which can be seen that, joined antibacterial peptide treated that fabric itself also improves in cloth characteristic.
Above-described embodiment is interpreted as being merely to illustrate the present invention rather than limit the scope of the invention.It is reading
After the content of the invention recorded, those skilled in the art can make various modifications or changes to the present invention, these equivalent changes
Change and modification equally falls into the scope of the claims in the present invention.
Sequence table
<110>Yichuan Xi Yu biological products Co., Ltd
<120>a kind of antibacterial fabric and preparation method thereof
<160> 5
<170> SIPOSequenceListing 1.0
<210> 1
<211> 27
<212> PRT
<213>artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)
<400> 1
His Pro Gln Met Val Asn Arg Pro Phe Tyr Gln Thr Arg Lys Arg Phe
1 5 10 15
Asp Ser Cys Pro Glu Ala His Pro Ala Arg Ile
20 25
<210> 2
<211> 27
<212> PRT
<213>artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)
<400> 2
Lys His Gln Arg Ile Glu Ala Pro Ser Arg His Tyr Trp Lys Arg Tyr
1 5 10 15
Arg Met Cys His Asp Tyr Trp Gln Ser Thr Lys
20 25
<210> 3
<211> 27
<212> PRT
<213>artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)
<400> 3
Arg Pro Pro Leu Thr Gln Asp Tyr His Met Pro Pro Gln Met Arg Leu
1 5 10 15
Leu Arg Trp Lys Lys Asp Thr Trp Ala Trp Val
20 25
<210> 4
<211> 27
<212> PRT
<213>artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)
<400> 4
Tyr Met Gln Asp Trp Pro Lys Glu Met Leu Pro His Phe Pro Asn Trp
1 5 10 15
Ala Asn Gln Cys Cys Trp Ser Gln Pro Pro Phe
20 25
<210> 5
<211> 27
<212> PRT
<213>artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)
<400> 5
His Ile Gln Glu Gln Glu Pro Tyr Met Tyr Ile Ser Ala Arg Lys Ala
1 5 10 15
Arg Phe Met Met His Ser Phe His Trp Lys Pro
20 25