CN109776626A - A method of preventing the discoloration of saliva aqueous acid - Google Patents
A method of preventing the discoloration of saliva aqueous acid Download PDFInfo
- Publication number
- CN109776626A CN109776626A CN201811641254.6A CN201811641254A CN109776626A CN 109776626 A CN109776626 A CN 109776626A CN 201811641254 A CN201811641254 A CN 201811641254A CN 109776626 A CN109776626 A CN 109776626A
- Authority
- CN
- China
- Prior art keywords
- acid
- saliva
- sialic acid
- aqueous acid
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Saccharide Compounds (AREA)
Abstract
The present invention provides a kind of methods for preventing the discoloration of saliva aqueous acid.This method comprises the following steps: the pH of saliva aqueous acid is adjusted to 5.0~12.0.After method of the invention, by treated, saliva aqueous acid is placed in low temperature environment, normal temperature environment or hot environment, and saliva aqueous acid will be always maintained at colourless solution, and highly stable, at least keep under normal temperature environment 2 years.
Description
Technical field
The present invention relates to field of industrialized production, more particularly, to a kind of method for preventing the discoloration of saliva aqueous acid.
Background technique
Sialic acid has the function of to improve intelligence, memory, antibacterial sterilization etc., using wide in medicine, foods and cosmetics
It is general.In these application fields, sialic acid sample is usually carried out in form of an aqueous solutions using this just needs saliva aqueous acid sample
Product must be very stable, not only the stabilization of content, while other various aspects such as appearance color etc. requires to keep stablizing.Saliva
Aqueous acid is colorless and transparent, but saliva aqueous acid is unstable, with the extension of time, if storage condition is improper,
The color of aqueous solution with can gradually change, become light yellow from colourless, then gradually deepen again, this has just seriously affected saliva
The application of liquid acid.
Summary of the invention
In view of the above-mentioned problems, the first object of the present invention is to provide a kind of method for preventing the discoloration of saliva aqueous acid,
This method comprises the following steps:
The pH of saliva aqueous acid is adjusted to 5.0~12.0.
After method of the invention, by treated, saliva aqueous acid is placed on low temperature environment, normal temperature environment or height
In warm environment, saliva aqueous acid will be always maintained at colourless solution, and highly stable, at least keep under normal temperature environment 2 years.
In a preferred embodiment of the invention, the pH of saliva aqueous acid is adjusted to 6.0~8.0.
In a preferred embodiment of the invention, the saliva aqueous acid is dissolved in water by sialic acid and is made；The saliva
The purity of liquid acid is 80%~99%.
In the present invention, which is preferably the saliva that the extracting method disclosed in CN201811372637.8 obtains
Acid.Wherein, all schemes disclosed in CN201811372637.8 are included in the present invention.
Wherein it is preferred to be, the extracting method of sialic acid of the invention is the saliva for pre-processing sialic acid monomer source
Liquid acid solution is adjusted pH, concentration purification, heating concentration and acidizing crystal and is obtained；The sialic acid monomer source is poly sialic acid
Or bird's nest.
The sialic acid monomer is preferably poly sialic acid, and the pretreatment is hydrolysis.The hydrolysis of the poly sialic acid
Further comprise: being added into poly sialic acid at a temperature of acid to pH value of solution is 1~2,80~90 DEG C and react 2~4h.Its
In, it is handled using poly sialic acid filtering fermentation liquor of the 50-300nm ceramic membrane to 10-30g/L, uses specification for 8-12KDa's afterwards
Ultrafiltration membrance filter is less than 3 times of poly sialic acid content to solution solid content, obtains above-mentioned poly sialic acid solution.Wherein, pH is adjusted
It is particularly preferred as: sodium hydroxide is added and is 5-7 to the pH of solution and obtains sialate solution.Concentration purification specifically includes: super
Filter processing and nanofiltration processing.Hyperfiltration treatment includes: to be filtered to remove not hydrolyzed to destainer using the ultrafiltration membrane of 0.5-2KDa
Full poly sialic acid.Nanofiltration processing are as follows: desalination is carried out to sialate solution using the nanofiltration membrane of 200-400Da, so that saliva
Solid content is less than 3 times of sialic acid content in acid salt solution.Heating concentration preferably includes: nanofiltration liquid is heated to concentration in a vacuum
8-12 times so that the concentration of sialic acid sodium reaches about 200-500g/L in solution after concentration.Acidizing crystal and drying include: to
It is 1-2 that acidic materials to pH value of solution is added in concentrate after heating concentration, and 5-10h is crystallized at 0-10 DEG C and obtains crystal,
Washing, vacuum drying obtain sialic acid after crystal is filtered.
In a preferred embodiment of the invention, the concentration of the saliva aqueous acid is 1%~20%, preferably
2%~5%.In a preferred embodiment of the invention, the pH of saliva aqueous acid is adjusted to 5.0 using alkaline solution~
12.0.Wherein, alkaline solution is sodium hydroxide, potassium hydroxide, sodium carbonate or potassium carbonate.
The pH value of saliva aqueous acid to 5.0~12.0 is placed in low temperature or normal temperature environment, is at least placed by the present invention
It will not change colour within 2 years, meanwhile, being sealed against being placed in 80 DEG C of environment will not change colour at least six moon, and sealing is placed on 60 DEG C of environment
In will not change colour at least 12 months.
Detailed description of the invention
Fig. 1 is experimental example 1 of the present invention the case where treated saliva aqueous acid room temperature 2 years；
Fig. 2 is comparative example 1 of the present invention the case where treated saliva aqueous acid room temperature 2 years
Fig. 3 is treated saliva aqueous acid seal at the 80 DEG C the case where placing of the embodiment of the present invention 2；
Fig. 4 is comparative example 2 of the present invention treated saliva aqueous acid seals at 80 DEG C the case where placing；
Fig. 5 is treated saliva aqueous acid seal at the 60 DEG C the case where placing of the embodiment of the present invention 3
For treated in comparative example 3 of the present invention, saliva aqueous acid seals the case where placing to Fig. 6 at 60 DEG C.
Specific embodiment
With reference to embodiment, the embodiment of the present invention is furthur described in detail.Following embodiment is used for
Illustrate the present invention, but is used to limit the scope of the invention incessantly.
The commercially available acquisition of raw material components used in the present invention, agents useful for same of the embodiment of the present invention are that chemistry is pure.
The preparation method of sialic acid used in the embodiment of the present invention is disclosed in embodiment 4 in CN201811372637.8
Method, it is specific as follows:
It is handled using poly sialic acid filtering fermentation liquor of the 200nm ceramic membrane to 25g/L, uses specification for the super of 11KDa afterwards
Membrane filtration is less than 3 times of poly sialic acid content to solution solid content, obtains poly sialic acid solution.Add into poly sialic acid solution
Enter sulfuric acid to pH value of solution be 1,86 DEG C at a temperature of reaction 3.0h obtain saliva acid solution.To the addition of saliva acid solution and saliva
The active carbon decolorization that acid solution mass ratio is 2% obtains destainer, and the pH that sodium hydroxide is added to solution is 5 and obtains
Sialate solution is filtered to remove destainer using the ultrafiltration membrane of 1.2KDa and does not hydrolyze complete poly sialic acid.Using 350Da
Nanofiltration membrane sialate solution is filtered to obtain nanofiltration liquid so that in nanofiltration liquid solid content be less than sialic acid content 3
Times.Heating in vacuum concentration is carried out to nanofiltration liquid, it is 2 that 33% perchloric acid to pH value of solution, which is then added, and 8h is crystallized at 6 DEG C and is tied
Brilliant object, crystal, which is filtered and is dried in vacuo, obtains sialic acid.
The present invention is described in detail with the sialic acid that embodiment 4 obtains in CN201811372637.8 in the present invention,
The result for the sialic acid that the sialic acid and embodiment 4 that other embodiment disclosed in CN201811372637.8 obtains obtain
Unanimously, it does not repeat one by one in the present invention.
Obtained sialic acid is dissolved in the saliva aqueous acid that water is made into various concentration in following embodiments.
Embodiment 1
Configuration 2% saliva aqueous acid, pH 2.0, then with sodium hydroxide adjust sialic acid pH value of water solution to
7.0, sealing is placed in normal temperature environment.It places 2 years and does not change colour.
Embodiment 2
The saliva aqueous acid of configuration 5%, pH 1.8 are close with potassium hydroxide adjusting sialic acid pH value of water solution to 8.0
Envelope is placed in 80 DEG C of environment.
Embodiment 3
N-acetyl-neuraminate pH value of water solution is adjusted to 9.0 with alkaline matters such as sodium hydroxide or sodium carbonate, and sealing is put
It sets in 60 DEG C of environment.
Comparative example 1
Comparative example 1 is same as Example 1, the difference is that, sodium hydroxide is not used and adjusts saliva aqueous acid pH.
Comparative example 2
Comparative example 2 is same as Example 2, the difference is that, potassium hydroxide is not used and adjusts saliva aqueous acid pH.
Comparative example 2
Comparative example 3 is same as Example 3, the difference is that, the alkaline matters tune such as sodium hydroxide or sodium carbonate is not used
Save saliva aqueous acid pH.
Saliva aqueous acid in embodiment 1, which is placed 2 years, not to change colour, as shown in Figure 1, and sialic acid is water-soluble in comparative example 1
Liquid about becomes light yellow when placing 6th month, as shown in Figure 2.
Saliva aqueous acid in embodiment 2, which is placed 6 months, not to change colour, as shown in figure 3, and saliva sour water in comparative example 2
Become light yellow at solution the 5th day, later color is gradually deepened, as shown in Figure 4.
Saliva aqueous acid in embodiment 3, which is placed 12 months, not to change colour, as shown in figure 5, and saliva sour water in comparative example 2
Become light yellow at solution the 15th day, later color is gradually deepened, as shown in Figure 6.
Finally, method of the invention is only preferable embodiment, it is not intended to limit the scope of the present invention.It is all
Within the spirit and principles in the present invention, any modification, equivalent replacement, improvement and so on should be included in protection of the invention
Within the scope of.
Claims (7)
1. a kind of method for preventing the discoloration of saliva aqueous acid, which comprises the steps of:
The pH of saliva aqueous acid is adjusted to 5.0~12.0.
2. the method according to claim 1, wherein the pH of saliva aqueous acid is adjusted to 6.0~8.0.
3. method according to claim 1 or 2, which is characterized in that the saliva aqueous acid is dissolved in water system by sialic acid
?；The purity of the sialic acid is at least 80%.
4. according to the method described in claim 3, it is characterized in that, the extracting method of the sialic acid is by sialic acid monomer source
Obtained saliva acid solution is pre-processed to obtain through adjusting pH, concentration purification, heating concentration and acidizing crystal；The sialic acid list
Body source is poly sialic acid or bird's nest.
5. according to the method described in claim 4, it is characterized in that, the sialic acid monomer is poly sialic acid, the pretreatment
For hydrolysis；The hydrolysis of the poly sialic acid include: into the poly sialic acid be added acid to pH value of solution be 1~2,80
2~4h is reacted at a temperature of~90 DEG C.
6. the method according to any one of claims 1 to 5, which is characterized in that the concentration of the saliva aqueous acid is
1%~20%, preferably 2%~5%.
7. method according to any one of claim 1 to 6, which is characterized in that using alkaline solution that sialic acid is water-soluble
The pH of liquid is adjusted to 5.0~12.0；The alkaline solution is sodium hydroxide, potassium hydroxide, sodium carbonate or potassium carbonate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811641254.6A CN109776626B (en) | 2018-12-29 | 2018-12-29 | Method for preventing sialic acid aqueous solution from discoloring |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811641254.6A CN109776626B (en) | 2018-12-29 | 2018-12-29 | Method for preventing sialic acid aqueous solution from discoloring |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109776626A true CN109776626A (en) | 2019-05-21 |
| CN109776626B CN109776626B (en) | 2020-06-23 |
Family
ID=66499548
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811641254.6A Active CN109776626B (en) | 2018-12-29 | 2018-12-29 | Method for preventing sialic acid aqueous solution from discoloring |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109776626B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111035581A (en) * | 2019-12-31 | 2020-04-21 | 嘉必优生物技术（武汉）股份有限公司 | Method for maintaining stability of sialic acid solution at high temperature and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5233033A (en) * | 1990-09-04 | 1993-08-03 | Taiyo Kagaku Co., Ltd. | Method for production of sialic acid |
| CN106366136A (en) * | 2016-08-03 | 2017-02-01 | 江南大学 | Sialic acid, and preparation method and application thereof |
| CN108659095A (en) * | 2018-05-18 | 2018-10-16 | 上海药明生物技术有限公司 | A method of so that sialic acid content is stablized |
-
2018
- 2018-12-29 CN CN201811641254.6A patent/CN109776626B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5233033A (en) * | 1990-09-04 | 1993-08-03 | Taiyo Kagaku Co., Ltd. | Method for production of sialic acid |
| CN106366136A (en) * | 2016-08-03 | 2017-02-01 | 江南大学 | Sialic acid, and preparation method and application thereof |
| CN108659095A (en) * | 2018-05-18 | 2018-10-16 | 上海药明生物技术有限公司 | A method of so that sialic acid content is stablized |
Non-Patent Citations (3)
| Title |
|---|
| ROLAND SCHAUER: "Achievements and challenges of sialic acid research", 《GLYCOCONJUGATE JOURNAL》 * |
| 吴剑荣 等: "聚唾液酸与唾液酸的研究进展", 《生物加工过程》 * |
| 李文强: ""唾液酸发酵控制及其分离纯化的研究"", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111035581A (en) * | 2019-12-31 | 2020-04-21 | 嘉必优生物技术（武汉）股份有限公司 | Method for maintaining stability of sialic acid solution at high temperature and application thereof |
| CN111035581B (en) * | 2019-12-31 | 2023-04-14 | 嘉必优生物技术（武汉）股份有限公司 | Method for maintaining stability of sialic acid solution at high temperature and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109776626B (en) | 2020-06-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| SU1215624A3 (en) | Method of producing polymer solution for extracting petroleum | |
| Brown | The peripheral structures of gram-negative bacteria: IV. The cation-sensitive dissolution of the cell membrane of the halophilic bacterium, Halobacterium halobium | |
| JPS603478B2 (en) | How to process xanthan gum | |
| CN109180745A (en) | A method of separating-purifying prepares N-acetyl-neuraminate from the material containing poly sialic acid | |
| CN109776626A (en) | A method of preventing the discoloration of saliva aqueous acid | |
| CN105837488B (en) | A kind of hydroxyproline fermentation manufacturing technique | |
| CN103965064A (en) | Method for extraction of L-alanine from L-alanine fermentation broth | |
| KR850001750A (en) | Method for preparing sulfo-adenosyl-L-methionine salt | |
| US5453200A (en) | Precifitation process for exocellular proteins | |
| WO2005090306A1 (en) | Process for the purification of tryptophan | |
| CN102660595A (en) | Preparation method of corn steep liquor | |
| US3728224A (en) | Enzyme purification process | |
| IE34250B1 (en) | Itaconic acid purification process using reverse osmosis | |
| CN204933271U (en) | A kind of succinic acid fermentation liquor separation and purification device | |
| Glenny et al. | Detection and concentration of antigens by ultrafiltration, pressure dialysis, etc. with special reference to diphtheria and tetanus toxins | |
| CN112746065A (en) | Method for removing pepsin endotoxin | |
| CN105668527A (en) | Method for preparing red selenium | |
| CN104724891B (en) | A kind of processing method of oxytetracycline crystallization mother liquor waste water | |
| CN101580586B (en) | Novel method for extracting polyglutamic acid | |
| US3238252A (en) | Purification of fermentation broths | |
| US3206506A (en) | Separation of acetylglutamine | |
| RU2261915C2 (en) | Method for preparing calcium citrate | |
| RU2209768C2 (en) | Method for preparing potassium sulfate | |
| RU1370856C (en) | Method of producing hyper-filtering polymeric diapharagms | |
| IE792114L (en) | Oil displacement xanthomonas product |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |