CN109771721A - The small preliminary filling reaction type extracorporal circulatory system pipeline of rat enclosed - Google Patents
The small preliminary filling reaction type extracorporal circulatory system pipeline of rat enclosed Download PDFInfo
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- CN109771721A CN109771721A CN201910261956.XA CN201910261956A CN109771721A CN 109771721 A CN109771721 A CN 109771721A CN 201910261956 A CN201910261956 A CN 201910261956A CN 109771721 A CN109771721 A CN 109771721A
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Abstract
The embodiment of the invention discloses a kind of small preliminary filling reaction type extracorporal circulatory system pipelines of rat enclosed, belong to experiment technical field of auxiliary equipment, its key points of the technical solution are that, the small preliminary filling reaction type extracorporal circulatory system pipeline of rat enclosed includes venous drainage pipe, blood storage chamber, rolling-type pumps connecting tube, heat exchanger, membrane oxygenator and artery diversion pipe, venous drainage pipe one end is for connecting rat vein, the other end is connect with blood storage chamber, blood storage chamber pumps connecting tube by rolling-type and connect with the blood inlet of the heat exchanger, the external rolling-type pump of rolling-type pump connecting tube, the blood outlet of the heat exchanger and the blood inlet of membrane oxygenator are connected by butt tube, the blood outlet of the membrane oxygenator connects the arterial puncture needle of rat by the artery diversion pipe, having reached is closed outer circulation pipeline, it is insufficient to avoid the occurrence of venous drainage amount, Blood storage chamber and other pipelines are easily emptied the effect of situations such as air inlet, pipeline entirety priming volume.
Description
Technical field
The present embodiments relate to experiment technical field of auxiliary equipment, and in particular to a kind of small preliminary filling reaction type of rat enclosed
Extracorporal circulatory system pipeline.
Background technique
There are the research in terms of doing rats in vitro circulation and hypothermia circulatory arrest model, animal in terms of department of cardiac surgery and severe medicine
Experiment is mostly that laboratory technician buys membrane lung, heat exchanger etc., and voluntarily builds pipeline, a set of finished product, it is efficient, easy to operate, low at
This pipeline has very big help to experiment progress, and lacks this kind of pipeline being simple and efficient at present.
Domestic and foreign literature report mostly uses greatly open rats in vitro to recycle at present, and priming volume is big, when model foundation
Between it is long, intermediary operation is cumbersome, and model success rate is low.Mainly there is following deficiency:
Open pipeline is directly communicated with atmosphere, is needed to lean on gravity drainage venous blood merely, be had the following deficiencies: 1. rat
It need to be placed at least 50cm height, and venous drainage pipe need to reach slightly to guarantee sufficient extracorporal circulatory system flow, however nonetheless,
Venous drainage amount is not still considerable；2. open system needs moment concern blood storage chamber to prevent from being emptied, several laboratory technicians is needed to operate,
It is cumbersome；3. open system is easy to be emptied air inlet, especially deep to stop the rear rewarming stage, modeling failure is directly resulted in, it is furthermore right
The high experiment of sterility requirements is also not suitable for；4. open system needs the moment to pay close attention to blood storage chamber liquid level, body temperature recycle stream is adjusted at any time
Amount, it is cumbersome and be unfavorable for control variable；5. due to the limitation of open system, it is necessary to the blood storage chamber of larger capacity and
Longer arterial blood and venous blood pipeline increase priming volume extremely, and rat hemochrome is too low in cyclic process, seriously affect and deposit
Living and experimental result；Due to open limitation, pipeline needs larger priming volume, and rat blood excess dilution influences Tissue Blood
For and metabolism, directly affect experiment success rate, large error will also result in experimental result, additionally due to pipeline priming volume increase
Greatly, in order to reduce preliminary filling, experimental group directly abandons access heat exchanger, this substantially reduces drop/heating efficiency, is also not easy essence
Really control temperature.
Summary of the invention
For this purpose, the embodiment of the present invention provides a kind of small preliminary filling reaction type extracorporal circulatory system pipeline of rat enclosed, it is existing to solve
Due to venous drainage amount deficiency, blood storage chamber and other pipelines caused by directly being communicated with atmosphere using open pipeline in technology
Easily it is emptied the larger problem of air inlet, pipeline entirety priming volume.
To achieve the goals above, the embodiment of the present invention provides the following technical solutions:
A kind of small preliminary filling reaction type extracorporal circulatory system pipeline of rat enclosed, the outer circulation pipeline include venous drainage pipe, storage
Blood room, rolling-type pump connecting tube, heat exchanger, membrane oxygenator and artery diversion pipe, venous drainage pipe one end is for connecting
Rat vein is connect, the other end is connect with blood storage chamber, and blood storage chamber pumps the blood inlet of connecting tube and the heat exchanger by rolling-type
Connection, the external rolling-type pump of rolling-type pump connecting tube, the blood outlet of the heat exchanger and the blood inlet of membrane oxygenator
It is connected by butt tube, the blood outlet of the membrane oxygenator connects the arterial puncture needle of rat by the artery diversion pipe.
Further, negative pressure is sealedly connected on the blood storage chamber to enrich blood device, negative pressure device of enriching blood includes casing
The piston rod being slidably connected in described sleeve pipe with sealing, described sleeve pipe are connected to by a connecting tube with blood storage chamber.
Further, the negative pressure enriches blood device as syringe, and the injection port of syringe is connected by connecting tube and blood storage chamber
It is logical.
Further, the negative pressure enrich blood device use 5ml injection capacity syringe.
Further, calparine cap is sealedly connected on the blood storage chamber.
Further, the venous drainage pipe connect one end with rat vein and is provided with three-way connection, the three-way connection
Two paths be respectively used to connection rat vein and venous drainage pipe, another access makes for venesection and cardiac arrest
With.
Further, the small preliminary filling reaction type extracorporal circulatory system pipeline entirety priming volume 7.5ml-8ml of the rat enclosed, it is described
Venous drainage pipe range 10cm, the rolling-type pump connection pipe range 24cm, the docking pipe range 4cm, the artery water conservancy diversion pipe range
20cm。
Further, the rolling-type pump connecting tube uses silica gel material.
The embodiment of the present invention has the advantages that
Using closed loop mode, the case where keeping venous drainage amount bigger, avoiding the occurrence of suction generation, operate it is simpler,
Recycle more stable, experiment success rate is higher；Due to being closed system, by vacuum suction, greatly reduce duct length, to subtract
Small priming volume.
Negative pressure is arranged on closed circuit to enrich blood device, the autonomous fluid infusion when negative pressure is excessive in pipeline keeps fluid infusion more objective, no
Need to adjust pump speed, significantly mitigation laboratory technician's burden, it is often more important that reduce experimental error, improve survival rate.
Detailed description of the invention
It, below will be to embodiment party in order to illustrate more clearly of embodiments of the present invention or technical solution in the prior art
Formula or attached drawing needed to be used in the description of the prior art are briefly described.It should be evident that the accompanying drawings in the following description is only
It is merely exemplary, it for those of ordinary skill in the art, without creative efforts, can also basis
The attached drawing of offer, which is extended, obtains other implementation attached drawings.
Structure depicted in this specification, ratio, size etc., only to cooperate the revealed content of specification, for
Those skilled in the art understands and reads, and is not intended to limit the invention enforceable qualifications, therefore does not have technical
Essential meaning, the modification of any structure, the change of proportionate relationship or the adjustment of size are not influencing the function of the invention that can be generated
Under effect and the purpose that can reach, should all still it fall in the range of disclosed technology contents obtain and can cover.
Fig. 1 is the small preliminary filling reaction type extracorporal circulatory system pipe connecting structure of a kind of rat enclosed that the embodiment of the present invention 1 provides
Schematic diagram.
In figure: 1, venous drainage pipe；11, three-way connection；2, blood storage chamber；21, negative pressure is enriched blood device；211, casing；212,
Piston rod；22, calparine cap；3, rolling-type pumps connecting tube；4, heat exchanger；5, membrane oxygenator；51, butt tube；6, artery is led
Flow tube；7, rolling-type pumps.
Specific embodiment
Embodiments of the present invention are illustrated by particular specific embodiment below, those skilled in the art can be by this explanation
Content disclosed by book is understood other advantages and efficacy of the present invention easily, it is clear that described embodiment is the present invention one
Section Example, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not doing
Every other embodiment obtained under the premise of creative work out, shall fall within the protection scope of the present invention.
A kind of small preliminary filling reaction type extracorporal circulatory system pipeline of rat enclosed, as shown in Figure 1, including venous drainage pipe 1, blood storage chamber
2, rolling-type pump connecting tube 3, heat exchanger 4, membrane oxygenator 5 and artery diversion pipe 6,1 one end of venous drainage pipe is for connecting
Rat vein, the other end are connect with blood storage chamber 2, can be used for for rat vein blood being drained in blood storage chamber 2, blood storage chamber 2 passes through rolling
Pressure type pump connecting tube 3 is connect with the blood inlet of heat exchanger 4, and rolling-type pumps the external rolling-type of connecting tube 3 pump 7, when rolling-type pumps 7
Can make the blood circulation in outer circulation pipeline when operating, the blood inlet of the blood outlet of heat exchanger 4 and membrane oxygenator 5 by pair
Adapter tube 51 connects, and the blood outlet of membrane oxygenator 5 connects the arterial puncture needle of rat by artery diversion pipe 6, passes through heat exchange
Device 4 and membrane oxygenator 5, which can be converted to venous blood, can directly input the intracorporal arterial blood of rat, realize extracorporal circulatory system.
Since outer circulation pipeline is using pipeline when being closed, venous drainage amount can be made bigger, and can be avoided and suction occur
The case where occur, the blood circulation in outer circulation pipeline by rolling-type pump 7 auxiliary carry out, by vacuum suction, greatly reduce
Duct length, to reduce priming volume.
Intraoperative hemorrhage situation, depth of anesthesia, drainage tube placement location accuracy etc. all influence venous drainage, furthermore in experiment
The physiology courses such as liquid tissue extravasation can occur for rat, and liquid is caused to be short of, so appropriate fluid infusion is needed in experimentation, due to
Rat is small in size, and amount infused relies on experience, and experimental differences are big, reduce experiment success rate, also directly affect experimental result, and
Still lack objective liquid replenishing method in the prior art.Opening is changed to closed system merely, easily causes venous drainage end negative pressure
Excessive, damage, which is even inhaled, breaks the right heart and superior and inferior vena cava, cannot objective observation drain situation, if in the duct again external one bear
Pressure table then greatly increases experimental cost.
To solve the above problems, the storage of the small preliminary filling reaction type extracorporal circulatory system pipeline of rat enclosed provided in an embodiment of the present invention
It is sealedly connected with negative pressure on blood room 2 to enrich blood device 21, is enriched blood automatically with realizing, negative pressure device 21 of enriching blood includes casing 211 and close
The piston rod 212 being slidably connected in casing 211 is sealed, the chamber indoor storage supplement constituted between casing 211 and piston rod 212
Liquid, casing 211 are connected to by a connecting tube with blood storage chamber 2, and when negative pressure is excessive in pipeline, piston rod 212 can overcome piston rod
Frictional force between 212 and casing 211, to realize autonomous fluid infusion, keeps fluid infusion more objective, is not required to 211 internal slide of casing
Pump speed is adjusted, significantly mitigation laboratory technician's burden, it is often more important that reduce experimental error, improve survival rate.
Preferably, negative pressure enriches blood device 21 as syringe, and the injection port of syringe is connected to by connecting tube with blood storage chamber 2,
Carrying out automatic liquid supply by syringe has materials conveniently, the low advantage of use cost, and the scale on syringe is also convenient for
It carries out quantitatively extracting replenisher in advance.
More preferably, negative pressure enriches blood device 21 using the syringe of 5ml injection capacity, and injection capacity is the syringe energy of 5ml
The replenisher of sufficient amount is enough stored, also, the frictional force of 5ml syringe can meet the maximum safety value of negative pressure just, make rat
It is easier to survive during the experiment.
Calparine cap 22 is sealedly connected on blood storage chamber 2, for mending alkali in cyclic process, it is also possible to make vasoactive agent etc.
Dosing channel.
Venous drainage pipe 1 connect one end with rat vein and is provided with three-way connection 11, the two paths of the three-way connection 11
It is respectively used to connection rat vein and venous drainage pipe 1, realizes the export function of venous blood, another access of three-way connection 11
In normally off, it can be used for venesection and cardiac arrest use, it is easy to use, it is easily operated, it needs to carry out venesection
With cardiac arrest using when have no need to change original pipeline of outer circulation pipeline and can be realized.
The whole priming volume of outer circulation pipeline is 7.5ml-8ml, and venous drainage pipe 1 is 10cm long, and it is long that rolling-type pumps connecting tube 3
24cm, butt tube 51 is 4cm long, and artery diversion pipe 6 is 20cm long, realize under the premise of outer circulation function with shorter pipeline and compared with
Small priming volume, to keep pipeline installation more convenient, rat hemochrome is too low in also avoidable cyclic process, seriously affects survival
And experimental result.
Rolling-type pumps connecting tube 3 and preferably uses silica gel material, has under the premise of meeting blood circulation use better
Durability, with rolling-type pump 7 carry out with the use of when be not susceptible to cracking failure situations such as, make to test safer, stability is more
It is good.
The small preliminary filling reaction type extracorporal circulatory system pipeline of a kind of rat enclosed provided by the invention, have easy for installation, cost at
This low advantage, is less prone to the case where suction is inflated in use, and the autonomous fluid infusion when negative pressure is excessive in pipeline increases
The survival rate of rat reduces the burden of experimenter.
Although above having used general explanation and specific embodiment, the present invention is described in detail, at this
On the basis of invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore,
These modifications or improvements without departing from theon the basis of the spirit of the present invention are fallen within the scope of the claimed invention.
Claims (8)
1. a kind of small preliminary filling reaction type extracorporal circulatory system pipeline of rat enclosed, which is characterized in that the extracorporal circulatory system pipeline includes quiet
Arteries and veins drainage tube (1), blood storage chamber (2), rolling-type pump connecting tube (3), heat exchanger (4), membrane oxygenator (5) and artery diversion pipe
(6), described venous drainage pipe (1) one end is for connecting rat vein, and the other end is connect with blood storage chamber (2), and blood storage chamber (2) passes through
Rolling-type pump connecting tube (3) is connect with the blood inlet of the heat exchanger (4), and the rolling-type pumps connecting tube (3) external rolling
Formula pumps (7), and the blood outlet of the heat exchanger (4) is connect with the blood inlet of membrane oxygenator (5) by butt tube (51), described
The blood outlet of membrane oxygenator (5) connects the arterial puncture needle of rat by the artery diversion pipe (6).
2. the small preliminary filling reaction type extracorporal circulatory system pipeline of rat enclosed according to claim 1, which is characterized in that the storage blood
Negative pressure is sealedly connected on room (2) to enrich blood device (21), the negative pressure enrich blood device (21) include casing (211) and sealing sliding
The piston rod (212) being connected in described sleeve pipe (211), described sleeve pipe (211) are connected to by a connecting tube with blood storage chamber (2).
3. the small preliminary filling reaction type extracorporal circulatory system pipeline of rat enclosed according to claim 2, which is characterized in that the negative pressure
Device (21) are enriched blood as syringe, the injection port of syringe is connected to by connecting tube with blood storage chamber (2).
4. the small preliminary filling reaction type extracorporal circulatory system pipeline of rat enclosed according to claim 2, which is characterized in that the negative pressure
Enrich blood device (21) use 5ml injection capacity syringe.
5. the small preliminary filling reaction type extracorporal circulatory system pipeline of rat enclosed according to claim 1, which is characterized in that the storage blood
Calparine cap (22) are sealedly connected on room (2).
6. the small preliminary filling reaction type extracorporal circulatory system pipeline of rat enclosed according to claim 1, which is characterized in that the vein
Drainage tube (1) connect one end with rat vein and is provided with three-way connection (11), the two paths difference of the three-way connection (11)
For connecting rat vein and venous drainage pipe (1), another access is used for venesection and cardiac arrest.
7. the small preliminary filling reaction type extracorporal circulatory system pipeline of rat enclosed according to claim 1, which is characterized in that the rat
The small preliminary filling reaction type extracorporal circulatory system pipeline entirety priming volume 7.5ml-8ml of enclosed, the venous drainage pipe (1) long 10cm, it is described
Rolling-type pumps connecting tube (3) long 24cm, the long 4cm of the butt tube (51), the long 20cm of the artery diversion pipe (6).
8. the small preliminary filling reaction type extracorporal circulatory system pipeline of rat enclosed according to claim 1, which is characterized in that the rolling
Formula pumps connecting tube (3) and uses silica gel material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910261956.XA CN109771721A (en) | 2019-04-02 | 2019-04-02 | The small preliminary filling reaction type extracorporal circulatory system pipeline of rat enclosed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910261956.XA CN109771721A (en) | 2019-04-02 | 2019-04-02 | The small preliminary filling reaction type extracorporal circulatory system pipeline of rat enclosed |
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| CN109771721A true CN109771721A (en) | 2019-05-21 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3069839U (en) * | 1999-12-21 | 2000-07-04 | 川澄化学工業株式会社 | Extracorporeal circulation circuit |
| CN107485742A (en) * | 2017-09-08 | 2017-12-19 | 重庆山外山血液净化技术股份有限公司 | The pipeline preliminary filling control method of blood purification system and the blood purification system |
| US20180289918A1 (en) * | 2015-10-16 | 2018-10-11 | Metran Co., Ltd. | Humidifier and respiratory assistance device |
-
2019
- 2019-04-02 CN CN201910261956.XA patent/CN109771721A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3069839U (en) * | 1999-12-21 | 2000-07-04 | 川澄化学工業株式会社 | Extracorporeal circulation circuit |
| US20180289918A1 (en) * | 2015-10-16 | 2018-10-11 | Metran Co., Ltd. | Humidifier and respiratory assistance device |
| CN107485742A (en) * | 2017-09-08 | 2017-12-19 | 重庆山外山血液净化技术股份有限公司 | The pipeline preliminary filling control method of blood purification system and the blood purification system |
Non-Patent Citations (3)
| Title |
|---|
| 李健: "无血预充大鼠体外循环模型的建立", 《中国体外循环杂志》, no. 03, pages 184 - 188 * |
| 谢小均;陶开宇;唐梦琳;杜磊;安琪;赁可;干昌平;陈佑雯;罗书画;: "大鼠体外循环模型的建立及其效果评价", 《四川大学学报(医学版)》, no. 05, pages 770 - 774 * |
| 黑飞龙;高国栋;周荣华;龙村;温复兴;关彬;史世勇;: "大鼠体外循环模型的建立", 《中国体外循环杂志》, no. 04, pages 224 - 227 * |
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| CB03 | Change of inventor or designer information |
Inventor after: Dong Aiqiang Inventor after: Zhu Xian Inventor after: Kong Minjian Inventor after: Wei Dongdong Inventor after: Wang Lifeng Inventor before: Wang Lifeng Inventor before: Zhu Xian |
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Effective date of registration: 20200728 Address after: 310058 Xihu District, Zhejiang, Yuhang Tong Road, No. 866, No. Applicant after: ZHEJIANG University Address before: 310000 Huajiachi Campus, Triumph Road, Jianggan District, Hangzhou City, Zhejiang Province Applicant before: Wang Lifeng |
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