CN109758602B - High suction type, strong antibacterial dressing and preparation method thereof - Google Patents
High suction type, strong antibacterial dressing and preparation method thereof Download PDFInfo
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- CN109758602B CN109758602B CN201910106018.2A CN201910106018A CN109758602B CN 109758602 B CN109758602 B CN 109758602B CN 201910106018 A CN201910106018 A CN 201910106018A CN 109758602 B CN109758602 B CN 109758602B
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Abstract
The present invention relates to pharmaceutical technology fields, and in particular to high suction type, strong antibacterial dressing and preparation method thereof.The dressing obtains after carrying out electrostatic spinning by spinning solution, and the spinning solution includes that there are two types of sorptivety compositions and bactericidal composition that the hydroxyethyl chitosan of different degree of substitution forms for tool.Dressing prepared by the present invention has the characteristics that high sorptivety ability, can quickly absorb wound fluid, can reach 50 times of original weight amount or more after water suction, and all have high inhibition effect to the multi-infection bacterium of the surface of a wound, achieves significant progress compared with prior art.
Description
Technical field
The present invention relates to pharmaceutical technology fields, and in particular to high suction type, strong antibacterial dressing and preparation method thereof.
Background technique
Chitosan due to its with biodegradability, broad spectrum antibacterial, no antigen and good biocompatibility, only
The functions such as blood and wound healing promoting, make it in medical field, are particularly suitable as absorbable surgical sewing thread, wound dressing
The various tissue engineering bracket materials such as hemostatic material.Wherein, it when being used as dressing, with good gas permeability, compliance, receives
Universal concern.However, the clinical test of many years proves, such dressing remains in use absorbs surface of a wound exudation
The phenomenon that liquid is slow and uptake is small, make the dressing the bleeding type surface of a wound and the burn type surface of a wound using when appearance effect is bad asks
Topic.
Secondly, some researchs are compound with dressing by antibacterial material in order to improve the anti-microbial property of chitosan dressing, make it
Obtain the dressing of permanent antibiotic effect.Wherein, the anti-biotic material of silver series is concerned in inorganic antibacterial field in recent years, such as
Fwu-LongMi etc. adds flamazine (AgSD) in chitosan film, and test proves that the film is to Pseudomonas aeruginosa and golden yellow
Glucose coccus has good inhibiting effect.But its antibacterial range is smaller, and for Burn Infection etc., surface of a wound table
Face mainly based on Acinetobacter baumannii, accounts for 39%, followed by proteus mirabilis and pseudomonas aeruginosa, is
20%, current Chitosan-Silver combine dressing is not strong for the fungistatic effect of such wound.
Therefore, it is necessary to which it is strong to provide a kind of sorptivety ability, and the dressing that scope of restraining fungi is wide.
Summary of the invention
In view of the above-mentioned problems of the prior art, the present invention is intended to provide a kind of high suction type, strong antibacterial dressing, the dressing
Can quick absorbing wound exudate, uptake is big, and the wide energy of scope of restraining fungi.
In order to achieve the above object, the invention adopts the following technical scheme: a kind of high suction type, strong antibacterial dressing, by spinning
Liquid obtains after carrying out electrostatic spinning, and the spinning solution includes that there are two types of the sorptivetys that the hydroxyethyl chitosan of different degree of substitution forms for tool
Composition and bactericidal composition.
Preferably, the sorptivety composition includes the hydroxyethyl chitosan that degree of substitution is 0.85 and the hydroxyl that degree of substitution is 0.3
Ethyl chitosan, the weight ratio of the two are 1:(0.01~0.1), it is highly preferred that the weight ratio of the two is 1:0.05.
Particularly, the molecular weight of the hydroxyethyl chitosan is preferably 20,000-10 ten thousand Da.
Preferably, the bactericidal composition includes silver sulfate and Surfactin, the weight ratio of the two be 1:(0.01~
1), it is highly preferred that the weight ratio of the two is 1:0.2.
Preferably, the sorptivety composition accounts for the 1~45% of dressing gross mass.
Preferably, the bactericidal composition accounts for the 0.1~5% of dressing gross mass.
Another object of the present invention is to provide a kind of method for preparing above-mentioned dressing, comprising the following steps:
A) hydroxyethyl chitosan is dissolved in the acetum of 0.1~2wt%, hydroxyethyl chitosan solution is prepared, with
The bactericidal composition aqueous solution of 3000~4000ppm mixes, and carries out homogenization；
B above-mentioned steps acquired solution) is subjected to electrostatic spinning, gained wet fiber silk is dried at 105~110 DEG C, is obtained
To shredded dried bean curd, shredded dried bean curd is subjected to needle thorn, obtains non-woven fabrics, be packaged, seal, irradiation sterilization to obtain the final product.
A key point of the invention is, forms sorptivety system using the hydroxyethyl chitosan of two kinds of different degree of substitution, can
The sorptivety ability of dressing is improved significantly.And this ability is related with the degree of substitution of hydroxyethyl chitosan.When using a kind of substitution
The hydroxyethyl chitosan of degree or other degree of substitution can not obtain above-mentioned technical effect.
Another key point of the invention is, antibacterial material is used as using Surfactin and silver sulfate are compound, through testing
It proves, which all has significant inhibiting effect to a variety of strains, and the two achieves synergistic technical effect.
The beneficial effects of the present invention are:
Dressing prepared by the present invention has the characteristics that high sorptivety ability, can quickly absorb wound fluid, and to the surface of a wound
Multi-infection bacterium all have high inhibition effect, achieve significant progress compared with prior art.
Specific embodiment
The specific embodiment of form by the following examples makees further specifically above content of the invention
It is bright.But the range that this should not be interpreted as to the above-mentioned theme of the present invention is only limitted to following embodiment.
Embodiment 1, high suction type, strong antibacterial dressing
It is obtained after carrying out electrostatic spinning by spinning solution, the spinning solution includes that there are two types of the ethoxy shells of different degree of substitution for tool
The sorptivety composition and bactericidal composition of glycan composition, the sorptivety composition include the hydroxyethyl chitosan that degree of substitution is 0.85
The hydroxyethyl chitosan for being 0.3 with degree of substitution, the two mass ratio are 1:0.05；The bactericidal composition includes silver sulfate and surface
Active extract, the two mass ratio are 1:0.2；Wherein the sorptivety composition accounts for the 30% of dressing gross mass, the bactericidal composition
Account for the 3% of dressing gross mass.
Preparation method:
A) hydroxyethyl chitosan is dissolved in the acetum of 1.0wt%, hydroxyethyl chitosan solution is prepared, with
The bactericidal composition aqueous solution of 4000ppm mixes, and carries out homogenization；
B above-mentioned steps acquired solution) is subjected to electrostatic spinning, gained wet fiber silk is dried at 110 DEG C, is done
Silk, by shredded dried bean curd carry out needle thorn, obtain non-woven fabrics, be packaged, seal, irradiation sterilization to obtain the final product.
Embodiment 2, high suction type, strong antibacterial dressing
It is obtained after carrying out electrostatic spinning by spinning solution, the spinning solution includes that there are two types of the ethoxy shells of different degree of substitution for tool
The sorptivety composition and bactericidal composition of glycan composition, the sorptivety composition include the hydroxyethyl chitosan that degree of substitution is 0.85
The hydroxyethyl chitosan for being 0.3 with degree of substitution, the weight ratio of the two are 1:0.01；The bactericidal composition includes silver sulfate and table
Face active extract, the weight ratio of the two are 1:0.5；Wherein the sorptivety composition accounts for the 25% of dressing gross mass, the antibacterial group
It closes object and accounts for the 2.5% of dressing gross mass.
Shown in preparation method reference implementation example 1.
Embodiment 3, high suction type, strong antibacterial dressing
It is obtained after carrying out electrostatic spinning by spinning solution, the spinning solution includes that there are two types of the ethoxy shells of different degree of substitution for tool
The sorptivety composition and bactericidal composition of glycan composition, the sorptivety composition include the hydroxyethyl chitosan that degree of substitution is 0.85
The hydroxyethyl chitosan for being 0.3 with degree of substitution, the weight ratio of the two are 1:0.1；The bactericidal composition includes silver sulfate and table
Face active extract, the weight ratio of the two are 1:0.4；Wherein the sorptivety composition accounts for the 40% of dressing gross mass, the antibacterial group
It closes object and accounts for the 5% of dressing gross mass.
Shown in preparation method reference implementation example 1.
Comparative example 1
The difference from embodiment 1 is that the sorptivety composition is the hydroxyethyl chitosan that degree of substitution is 0.85, remaining ginseng
Number and operation are as described in Example 1.
Comparative example 2
The difference from embodiment 1 is that the sorptivety composition is the hydroxyethyl chitosan that degree of substitution is 0.3, remaining ginseng
Number and operation are as described in Example 1.
Comparative example 3
The difference from embodiment 1 is that the sorptivety composition includes the hydroxyethyl chitosan and substitution that degree of substitution is 0.8
The hydroxyethyl chitosan that degree is 0.3, the weight ratio of the two are 1:0.05, remaining parameter and operation are as described in Example 1.
Comparative example 4
The difference from embodiment 1 is that the bactericidal composition is silver sulfate solution, remaining parameter and operation such as embodiment
Shown in 1.
Comparative example 5
The difference from embodiment 1 is that the bactericidal composition is Surfactin, remaining parameter and operation such as embodiment
Shown in 1.
Test example one, in vitro toxicity test
The dressing of Example 1~3, it is right according to the 5th part of GBT16886.5-2003 BiologicalEvaluationofMedicalDevice
It carries out in vitro toxicity test, and test result meets the requirements.
Test example two, water absorption rate test
The dressing that Example 1~3 and comparative example 1~3 are prepared, reference common alginic acid Medical coating in the world
Expect that water absorption rate surveys method, the water absorption rate of various dressing is tested.It is big that 5 × 5cm is respectively cut into above-mentioned each group dressing
Small, being placed on temperature is 20 DEG C, under conditions of 65% relative humidity for 24 hours, so that the regain of dressing is reached balance, measures dressing
Dry weight (W, unit g).Dressing is placed on later in simulation wound fluid of 60 times than dressing weight (its be formulated such as the following table 1 institute
Show), after placing 30min in the culture dish that diameter is 90cm, one jiao of dressing is clamped with tweezers hang to measure after 30s in the sky and apply
Weight in wet base (the W of material1, unit g).The water absorbent rate of unit of account weight dressing are as follows: W1/ W, 10 samples of every group of measurement, record knot
Fruit, analysis, as shown in table 2 below.
Table 1 simulates wound fluid
| Ingredient | Relative molecular mass | Content |
| Ethyl lactate | 118.13 | 0.222% |
| Glucose | 186.11 | 0.013% |
| Protein | 2.9% | |
| Kreatinin | 113.12 | 1.187% |
| Urea | 61.05 | 0.0586% |
| Calcium ion is (with CaCl22H2O is represented) | 147.02 | 0.0367% |
| Sodium ion (is represented) with NaCl | 58.44 | 0.83% |
2 hygroscopicity testing result of table
Note: compared with example 1 group,*P < 0.05,**P < 0.01.
By upper table 2 it is found that the dressing being prepared using the hydroxyethyl chitosan of two kinds of different degree of substitution, water absorption rate are reachable
To 48 times or more of original weight amount, 50 times are reached as high as.And it is surprising that poly- using the ethoxy shell of one of degree of substitution
The dressing water absorption rate that sugar is prepared has extremely significant decline (P < 0.01) compared with example 1 group.Meanwhile when using substitution
Two kinds of hydroxyethyl chitosan compound tenses that degree is 0.8 and 0.3, compared with Example 1, water absorption rate also has obviously for obtained dressing
Decline (P < 0.05).
Test example three, bacteriostasis property test
Example 1~3 and the dressing of comparative example 4~5, with reference to GB15979-202 " Disposable Sanitary Accessory
Sanitary standard " to each group dressing progress antibacterial tests, detection strain also wraps appendix C 5 in addition to staphylococcus aureus, Pseudomonas aeruginosa
Acinetobacter baumannii, proteus mirabilis and pseudomonas aeruginosa are included, test result is as follows shown in table 3.
3 test result of table
By upper table 3 it is found that by silver sulfate and the compound dressing being prepared as antibacterial agent of Surfactin to golden yellow
Staphylococcus, Pseudomonas aeruginosa, Acinetobacter baumannii, proteus mirabilis and pseudomonas aeruginosa all have significant inhibition
Effect, bacteriostasis rate reach 95% or more.And when the dressing that antibacterial agent is silver sulfate preparation, to staphylococcus aureus, green pus
The inhibitory effect of bacillus no significant difference compared with Example 1, but to Acinetobacter baumannii, proteus mirabilis and verdigris
Pseudomonas alba inhibitory effect is substantially reduced；Surfactin to the inhibitory effect of staphylococcus aureus, Pseudomonas aeruginosa not
Obviously, Acinetobacter baumannii, proteus mirabilis and pseudomonas aeruginosa unrestraint are acted on.
For those skilled in the art, it can make other each according to the above description of the technical scheme and ideas
Kind is corresponding to be changed and deforms, and all these change and deform the protection model that all should belong to the claims in the present invention
Within enclosing.
Claims (6)
1. high suction type, strong antibacterial dressing, obtain after carrying out electrostatic spinning by spinning solution, which is characterized in that the spinning solution includes
There are two types of sorptivety compositions and bactericidal composition that the hydroxyethyl chitosan of different degree of substitution forms for tool；The sorptivety composition packet
Containing degree of substitution be 0.85 hydroxyethyl chitosan and degree of substitution be 0.3 hydroxyethyl chitosan, the weight ratio of the two is 1:(0.01
~0.1)；The bactericidal composition includes silver sulfate and Surfactin, and the weight ratio of the two is 1:(0.2~0.5).
2. high suction type as described in claim 1, strong antibacterial dressing, which is characterized in that the ethoxy that the degree of substitution is 0.85
The weight ratio for the hydroxyethyl chitosan that chitosan and degree of substitution are 0.3 is 1:0.05.
3. high suction type as described in claim 1, strong antibacterial dressing, which is characterized in that the silver sulfate and Surfactin
Weight ratio is 1:0.2.
4. dressing as described in claim 1, which is characterized in that the sorptivety composition accounts for the 1~45% of dressing gross mass.
5. dressing as described in claim 1, which is characterized in that the bactericidal composition accounts for the 0.1~5% of dressing gross mass.
6. a kind of method for preparing the dressing as described in Claims 1 to 5 is any, which comprises the following steps:
A) hydroxyethyl chitosan is dissolved in the acetum of 0.1~2wt%, hydroxyethyl chitosan solution is prepared, with 3000
The bactericidal composition aqueous solution of~4000ppm mixes, and carries out homogenization；
B above-mentioned steps acquired solution) is subjected to electrostatic spinning, gained wet fiber silk is dried at 105~110 DEG C, is done
Silk, by shredded dried bean curd carry out needle thorn, obtain non-woven fabrics, be packaged, seal, irradiation sterilization to obtain the final product.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101297976A (en) * | 2008-06-18 | 2008-11-05 | 天津大学 | Preparation of antibacterial silver/chitosan nano fiber membrane |
| CN103394114A (en) * | 2013-07-03 | 2013-11-20 | 东华大学 | Method for preparing chitosan-based superfine fiber carrier material for medical dressing |
| CN103705969A (en) * | 2014-01-02 | 2014-04-09 | 东华大学 | Method for preparing chitosan-based silver-loaded composite antimicrobial superfine fiber membrane |
| CN103993422A (en) * | 2014-04-23 | 2014-08-20 | 东华大学 | Regenerative silk fibroin/chitosan derivative blend fibrofelt and preparing method thereof |
-
2019
- 2019-01-31 CN CN201910106018.2A patent/CN109758602B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101297976A (en) * | 2008-06-18 | 2008-11-05 | 天津大学 | Preparation of antibacterial silver/chitosan nano fiber membrane |
| CN103394114A (en) * | 2013-07-03 | 2013-11-20 | 东华大学 | Method for preparing chitosan-based superfine fiber carrier material for medical dressing |
| CN103705969A (en) * | 2014-01-02 | 2014-04-09 | 东华大学 | Method for preparing chitosan-based silver-loaded composite antimicrobial superfine fiber membrane |
| CN103993422A (en) * | 2014-04-23 | 2014-08-20 | 东华大学 | Regenerative silk fibroin/chitosan derivative blend fibrofelt and preparing method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 水溶性N,O-羟乙基壳聚糖的合成;王婷等;《化学研究与应用》;20180315;第30卷(第3期);第396页左栏第7-9行、第397-398页"2.1 正交实验条件与结果分析"部分 * |
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Inventor after: Zhang Shuyue Inventor after: Jian Mingli Inventor after: Li Shuang Inventor after: Gao Peijun Inventor after: Liu Longqing Inventor before: Zhang Shuyue Inventor before: Gao Peijun Inventor before: Liu Longqing Inventor before: Jian Mingli Inventor before: Li Shuang |
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