CN109757608A - The preparation method of application and additive, feed and feed of the polyP bacteria in feed - Google Patents
The preparation method of application and additive, feed and feed of the polyP bacteria in feed Download PDFInfo
- Publication number
- CN109757608A CN109757608A CN201910281124.4A CN201910281124A CN109757608A CN 109757608 A CN109757608 A CN 109757608A CN 201910281124 A CN201910281124 A CN 201910281124A CN 109757608 A CN109757608 A CN 109757608A
- Authority
- CN
- China
- Prior art keywords
- bacteria
- feed
- polyp bacteria
- acid
- nutrient medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/195—Antibiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Inorganic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Fodder In General (AREA)
Abstract
The preparation method of the application and additive, feed and feed that the present invention relates to polyP bacterias in feed, which is characterized in that polyP bacteria is applied in feed.It is excessively high that the present invention is able to suppress organic acid in animal body, reduce organic acid it is excessively high caused by liver, gastrointestinal disease, improve animal immunizing power.
Description
Technical field
The preparation method of the application and additive, feed and feed that the present invention relates to polyP bacterias in feed.
Background technique
Fat content is higher in existing feed, generally reaches 8% or more, although birds, domestic animals and water can be improved quickly
The speed of growth of production, but the liver and gallbladder of birds, domestic animals and aquatic products, excess accumulation fatty in enteron aisle are also be easy to cause, cause fat
The problems such as liver, loose fish belly.The excess accumulation of fat in vivo can make the Nutrition and Metabolism of birds, domestic animals and aquatic products lack of proper care, and influence
The metabolism of nutriment absorbs, and influences birds, domestic animals and aquatic products growth, feed coefficient is caused to increase；Birds, domestic animals can also be made
With the immune system disorder of aquatic products, the disease resistance of birds, domestic animals and aquatic products is caused to decline, it is easier to break out large-scale infection
Property disease such as hemorrhage and enteritis etc., to cause the death of large area.
The organic acids such as cholic acid, phytic acid, ascorbic acid and malic acid are in the expansion of animal body internal emulsification fat and lipase
Contact area；The activity of regulation pancreatic lipase and lipoproteinesterase improves it and is metabolized to the hydrolysis of fat；It is transported in enteron aisle
Fat promotes the absorption of fat.In addition, activity of the organic acid by regulation hormone-sensitive lipase, hence it is evident that reduce from body fat
The catabolism of fat.In conclusion organic acid can save energy feedstuff, capacity usage ratio is improved, improves growth performance and Tu
Performance is killed, is " positive energy " to economize on resources.
Be added since Long term Animal is edible cholic acid, phytic acid, ascorbic acid and malic acid feed, have part dynamic
Long-term organic acid is exceeded in object, and physical environment is unbalance, Yi Yinqi liver, gastrointestinal disease, such as the excessively high caused liver of bile acid
Hardening, cholestasia, gestational cholestasia, gastric ulcer, small intestine dirt syndrome, enteritis disease and colonic diseases, in turn
Cause growth of animal slow, or even dead.
Lack in the market and is able to suppress the excessively high product of organic acid in animal body；In the market also without can both promote animal
Growth, and product that can be excessively high to avoid organic acid in animal body.
Our company combines Zhaoyuan City, animal and veterinary station, Mt. Qishan and Zhaoyuan City summer pasture animal and veterinary with Zhaoyuan City's livestock husbandry veterinarian general station
It stands, for this product of animal liver and gallbladder gastrointestinal disease problem joint development.
Summary of the invention
The present invention provides feed and preparation method thereof, solves technical problem: (1) liver and gallbladder stomach disease caused by organic acid is excessively high
Disease；(2) organic acid in growth of animal and animal body can be promoted excessively high.
In order to solve the above-mentioned technical problem, the invention adopts the following technical scheme:
Application of the polyP bacteria in feed.
A kind of feed addictive includes polyP bacteria.
The polyP bacteria and its product are harmless to animal.
Preferably, two kinds also containing one of acid-producing bacteria, organic acid and enzyme or arbitrary proportion and its more than；
The acid-producing bacteria includes the two or more of one of lactic acid bacteria, clostridium, saccharomycete and bacillus or arbitrary proportion；Institute
Belong to enzyme include two kinds of one of phytase, cellulase, protease and 7 α hydroxylases or arbitrary proportion and its more than；It is described
Organic acid include two kinds of one of bile acid, amino acid, lactic acid and malic acid or arbitrary proportion and its more than.
Preferably, the polyP bacteria is cultivated according to the following steps:
PolyP bacteria kind daughter bacteria is seeded in fluid nutrient medium and cultivates a period of time to get liquid polyP bacteria；Or
After the drying of liquid polyP bacteria, solid polyP bacteria is obtained；
The pH of the fluid nutrient medium is 7.0~7.4；The incubation time is 20~48h；Culture is aerobic culture；PolyP bacteria
The mass ratio of kind daughter bacteria and fluid nutrient medium is 1~10:100；The drying temperature is 30~80 DEG C；
Contain phosphorus in the fluid nutrient medium；
The phosphorus is phosphate buffer, and phosphate buffer pH is 7.0~7.4.
Preferably, fluid nutrient medium is 3~5 parts of peptone, and beef leaches 3~5 parts of object, potassium dihydrogen phosphate buffer solution
800~1000 parts, 12~15 parts of agar.
A kind of feed includes polyP bacteria.
Preferably, two kinds also containing one of acid-producing bacteria, organic acid and enzyme or arbitrary proportion and its more than；
The acid-producing bacteria includes the two or more of one of lactic acid bacteria, clostridium, saccharomycete and bacillus or arbitrary proportion；Institute
Belong to enzyme include two kinds of one of phytase, cellulase, protease and 7 α hydroxylases or arbitrary proportion and its more than；It is described
Organic acid include two kinds of one of bile acid, amino acid, lactic acid and malic acid or arbitrary proportion and its more than.
Preferably, the polyP bacteria is cultivated according to the following steps:
PolyP bacteria kind daughter bacteria is seeded in fluid nutrient medium and cultivates a period of time to get liquid polyP bacteria；Or
After the drying of liquid polyP bacteria, solid polyP bacteria is obtained；
The pH of the fluid nutrient medium is 7.0~7.4；The incubation time is 20~48h；Culture is aerobic culture；PolyP bacteria
The mass ratio of kind daughter bacteria and fluid nutrient medium is 1~10:100；The drying temperature is 30~80 DEG C；
Contain phosphorus in the fluid nutrient medium；
The phosphorus is phosphate buffer, and phosphate buffer pH is 7.0~7.4.
Preferably, fluid nutrient medium is 3~5 parts of peptone, and beef leaches 3~5 parts of object, potassium dihydrogen phosphate buffer solution
800~1000 parts, 12~15 parts of agar.
A kind of preparation method of feed, feed addictive is added in feed to get feed.
Invention has following advantageous effects:
1. polyP bacteria of the present invention is cultivated under aerobic condition, Phos is accumulated in vivo in the form of poly- phosphorus, work as polyP bacteria
After in into animal body, condition is anaerobism at this time, and polyP bacteria decomposes the poly- phosphorus gathered in vivo, decomposes the energy of generation
(adenosine triphosphate ATP) a part is survived for polyP bacteria, and another part energy (reduced coenzyme NADH) supplies
PolyP bacteria active absorption organic acid, and organic acid is stored in vivo in the form of poly-β-hydroxybutyric acid, decompose the Phos of generation
It is discharged into animal body, so that it is excessively high to achieve the purpose that reduce organic acid in animal body.
2. the application fluid nutrient medium uses phosphate buffer solution, Phos is absorbed under polyP bacteria aerobic condition, by phosphorus
Hydrochlorate buffer solution is inhaled in internal；After polyP bacteria enters in animal body, condition is anaerobism at this time, and polyP bacteria is poly- by what is gathered in vivo
Phosphorus decomposes, and discharges Phos, and Phos forms microenvironment buffer system in animal body, contains to reduce organic acid in animal body
Amount, liver and gallbladder gastrointestinal disease caused by avoiding organic acid excessively high.
3. the application can be by the mass ratio of adjusting polyP bacteria and acid-producing bacteria or/and organic acid, so that reaching can promote
Into growth of animal, and it is avoided that the effect that organic acid is excessively high in animal body.
Specific embodiment
The present invention is further illustrated below with reference to specific example.
Embodiment 1
Feed, the composition containing polyP bacteria and bile acid；
PolyP bacteria is purchased in Chinese industrial Microbiological Culture Collection administrative center number CICC 22102.
The polyP bacteria is cultivated according to the following steps:
PolyP bacteria kind daughter bacteria is seeded in fluid nutrient medium and cultivates a period of time to get liquid polyP bacteria, by liquid polyP bacteria
After drying, solid polyP bacteria is obtained.
The pH of the fluid nutrient medium is 7.0；The incubation time is for 24 hours；Culture is aerobic culture；PolyP bacteria kind daughter bacteria
Mass ratio with fluid nutrient medium is 10:100.Fluid nutrient medium is 4 parts of peptone, and beef leaches 4 parts of object, and potassium dihydrogen phosphate is slow
Rush 1000 parts of solution, 14 parts of agar；Phosphate buffer pH is 7.0.
To get feed after polyP bacteria, bile acid and premix are mixed according to mass ratio 1:0.007:998.993.
Premix proportion: 60 parts of dregs of beans, 10 parts of bone meal, 15 parts of fish meal, 5 parts of shrimp med, 3 parts of fish oil, 6 parts of corn flour, chlorination
0.1 part of choline, 0.3 part of multi-vitamins, 0.2 part of compound amino acid and 0.4 part of berberine.
Embodiment 2
Feed, the composition containing polyP bacteria and bile acid；
PolyP bacteria is purchased in Chinese industrial Microbiological Culture Collection administrative center number CICC 22102.
The polyP bacteria is cultivated according to the following steps:
PolyP bacteria kind daughter bacteria is seeded in fluid nutrient medium and cultivates a period of time to get liquid polyP bacteria, by liquid polyP bacteria
After drying, solid polyP bacteria is obtained.
The pH of the fluid nutrient medium is 7.0；The incubation time is for 24 hours；Culture is aerobic culture；PolyP bacteria kind daughter bacteria
Mass ratio with fluid nutrient medium is 10:100.Fluid nutrient medium is 4 parts of peptone, and beef leaches 4 parts of object, and potassium dihydrogen phosphate is slow
Rush 1000 parts of solution, 14 parts of agar；Phosphate buffer pH is 7.0.
To get feed after polyP bacteria, bile acid and premix are mixed according to mass ratio 1:0.006:998.994.
Premix proportion: 65 parts of corn, 15 parts of dregs of beans, 5 parts of stone grain, cotton dregs 10 divide, 3 parts of hydrolyzed feather meal, calcium monohydrogen phosphate 1
It is part, 0.8 part of rice bran oil, 0.15 part of lysine, 0.15 part of choline chloride, 0.3 part of multi-vitamins, 0.1 part of microelement, compound
0.2 part and 0.1 part of sodium bicarbonate of amino acid.
Embodiment 3
Feed is the composition of pig premix, liquid polyP bacteria and bile acid according to mass ratio 998:1:1；
The liquid polyP bacteria is cultivated according to the following steps:
PolyP bacteria kind daughter bacteria is seeded in fluid nutrient medium and cultivates a period of time to get liquid polyP bacteria.
The pH of the fluid nutrient medium is 7.0；The incubation time is for 24 hours；Culture is aerobic culture；PolyP bacteria kind daughter bacteria
Mass ratio with fluid nutrient medium is 10:100.Fluid nutrient medium is 4 parts of peptone, and beef leaches 4 parts of object, and potassium dihydrogen phosphate is slow
Rush 1000 parts of solution, 14 parts of agar；Phosphate buffer pH is 7.0.
Bile acid is added to liquid polyP bacteria to dissolve, to get feed in the premix being then added.
Pig premix is purchased in Hebei great Wu agriculture and animal husbandry Group Co., Ltd.
Embodiment 4
Feed is the composition of premix, phytase, malic acid and polyP bacteria according to mass ratio 993:1:2:4；
The polyP bacteria is cultivated according to the following steps:
PolyP bacteria kind daughter bacteria is seeded in fluid nutrient medium and cultivates a period of time to get liquid polyP bacteria, by liquid polyP bacteria
After drying, solid polyP bacteria is obtained.
The pH of the fluid nutrient medium is 7.2；The incubation time is for 24 hours；Culture is aerobic culture；PolyP bacteria kind daughter bacteria
Mass ratio with fluid nutrient medium is 10:100.Fluid nutrient medium is 3 parts of peptone, and beef leaches 5 parts of object, and potassium dihydrogen phosphate is slow
Rush 900 parts of solution, 12~15 parts of agar；Phosphate buffer pH is 7.2；Drying temperature is 60 DEG C.
Premix, phytase, malic acid and polyP bacteria are mixed, to get feed after packaging.
The premix buying is in Hebei great Wu agriculture and animal husbandry Group Co., Ltd.
Embodiment 5
Feed is the composition of premix, liquid polyP bacteria and liquid acid-producing bacteria according to mass ratio 999:0.5:0.5；
The liquid polyP bacteria is cultivated according to the following steps:
PolyP bacteria kind daughter bacteria is seeded in fluid nutrient medium and cultivates a period of time to get liquid polyP bacteria.
The pH of the fluid nutrient medium is 7.3；The incubation time is 20h；Culture is aerobic culture；PolyP bacteria kind daughter bacteria
Mass ratio with fluid nutrient medium is 10:100.Fluid nutrient medium is 3~5 parts of peptone, and beef leaches 3~5 parts of object, di(2-ethylhexyl)phosphate
800~1000 parts of hydrogen potassium buffer solution, 12~15 parts of agar；Phosphate buffer pH is 7.3.
Liquid acid-producing bacteria follows the steps below:
Acid-producing bacteria kind daughter bacteria is seeded in fluid nutrient medium and cultivates a period of time to get liquid acid-producing bacteria；
The pH of the fluid nutrient medium is 6.8；The incubation time is for 24 hours；Culture is Anaerobic culturel；Acid-producing bacteria kind daughter bacteria and liquid
The mass ratio of body culture medium is 10:100.Fluid nutrient medium be 3~5 parts of corn flour, 0.1~0.3 part of peptone, glucose 1~2
Part part, 12~15 parts of agar, distilled water 1L.
The polyP bacteria buying is in Chinese industrial Microbiological Culture Collection administrative center number CICC 22102.
The acid-producing bacteria buying is in Chinese industrial Microbiological Culture Collection administrative center number CICC 10350.
Calcium formate is added after premix, liquid polyP bacteria and liquid acid-producing bacteria are mixed to get feed.
The premix buying is in Hebei great Wu agriculture and animal husbandry Group Co., Ltd.
Embodiment 6
Feed contains acid-producing bacteria and polyP bacteria；
The polyP bacteria is cultivated according to the following steps:
PolyP bacteria kind daughter bacteria is seeded in fluid nutrient medium and cultivates a period of time to get liquid polyP bacteria, by liquid polyP bacteria
After drying, solid polyP bacteria is obtained.
The pH of the fluid nutrient medium is 7.4；The incubation time is 30h；Culture is aerobic culture；PolyP bacteria kind daughter bacteria
Mass ratio with fluid nutrient medium is 10:100.Fluid nutrient medium is 5 parts of peptone, and beef leaches 3 parts of object, and potassium dihydrogen phosphate is slow
Rush 1000 parts of solution, 12 parts of agar；Phosphate buffer pH is 7.4；Drying temperature is 50 DEG C.
Premix, acid-producing bacteria and polyP bacteria are mixed, to get feed after packaging.
The premix buying is in Hebei great Wu agriculture and animal husbandry Group Co., Ltd.
Embodiment 7
Feed contains glutamic acid and polyP bacteria；
The polyP bacteria and its product are to animal nonhazardous；
Contain phosphorus in the polyP bacteria culture medium.
Glutamic acid and polyP bacteria are added to after mixing in premix to get feed by the preparation method of feed；Or
Glutamic acid and polyP bacteria and premix are mixed, to get feed after being granulated.
Embodiment 8
Feed contains polyP bacteria and malic acid；
The polyP bacteria and its product are to animal nonhazardous.
The preparation method of feed mixes polyP bacteria, malic acid and premix to get feed；PolyP bacteria, malic acid and pre-
The mass ratio of mixing is 2:0.8:997.2.
The premix buying is in Shandong six and feed limited liability company.
Embodiment 9
Feed contains polyP bacteria.
The polyP bacteria and its product are to animal nonhazardous.
Embodiment 10
Feed contains polyP bacteria and malic acid；
The polyP bacteria and its product are to animal nonhazardous；
Contain sodium pyrophosphate in the culture medium of the polyP bacteria.
The preparation method of feed mixes polyP bacteria, malic acid and premix to get feed；PolyP bacteria, malic acid and pre-
The mass ratio of mixing is 2:0.8:997.2.
The premix buying is in Shandong six and feed limited liability company.
Embodiment 11
Feed addictive is liquid polyP bacteria；
It purchases in Chinese industrial Microbiological Culture Collection administrative center number CICC 22102.
The liquid polyP bacteria is cultivated according to the following steps:
PolyP bacteria kind daughter bacteria is seeded in fluid nutrient medium and cultivates a period of time to get liquid polyP bacteria.
The pH of the fluid nutrient medium is 7.0；The incubation time is for 24 hours；Culture is aerobic culture；PolyP bacteria kind daughter bacteria
Mass ratio with fluid nutrient medium is 10:100.Fluid nutrient medium is 4 parts of peptone, and beef leaches 4 parts of object, and potassium dihydrogen phosphate is slow
Rush 1000 parts of solution, 14 parts of agar；Phosphate buffer pH is 7.0.
To get feed addictive after liquid polyP bacteria is packed.
Embodiment 12
Feed addictive is the composition of liquid polyP bacteria and bile acid according to mass ratio 1:1；
The liquid polyP bacteria is cultivated according to the following steps:
PolyP bacteria kind daughter bacteria is seeded in fluid nutrient medium and cultivates a period of time to get liquid polyP bacteria.
The pH of the fluid nutrient medium is 7.0；The incubation time is for 24 hours；Culture is aerobic culture；PolyP bacteria kind daughter bacteria
Mass ratio with fluid nutrient medium is 10:100.Fluid nutrient medium is 4 parts of peptone, and beef leaches 4 parts of object, and potassium dihydrogen phosphate is slow
Rush 1000 parts of solution, 14 parts of agar；Phosphate buffer pH is 7.0.
Bile acid is added to the dissolution of liquid polyP bacteria to get feed addictive.
Embodiment 13
Feed addictive is the composition of calcium formate, sodium Diacetate, white vitriol and polyP bacteria according to mass ratio 1:1:1:2；
The polyP bacteria is cultivated according to the following steps:
PolyP bacteria kind daughter bacteria is seeded in fluid nutrient medium and cultivates a period of time to get liquid polyP bacteria, by liquid polyP bacteria
After drying, solid polyP bacteria is obtained.
The pH of the fluid nutrient medium is 7.2；The incubation time is for 24 hours；Culture is aerobic culture；PolyP bacteria kind daughter bacteria
Mass ratio with fluid nutrient medium is 10:100.Fluid nutrient medium is 3 parts of peptone, and beef leaches 5 parts of object, and potassium dihydrogen phosphate is slow
Rush 900 parts of solution, 12~15 parts of agar；Phosphate buffer pH is 7.2；Drying temperature is 60 DEG C.
Calcium formate, sodium Diacetate, white vitriol and polyP bacteria are mixed, to get feed addictive after packaging.
Embodiment 14
Feed addictive is the combination of liquid polyP bacteria, liquid acid-producing bacteria, phytase and calcium formate according to mass ratio 1:1:1:1
Object；
The liquid polyP bacteria is cultivated according to the following steps:
PolyP bacteria kind daughter bacteria is seeded in fluid nutrient medium and cultivates a period of time to get liquid polyP bacteria.
The pH of the fluid nutrient medium is 7.3；The incubation time is 20h；Culture is aerobic culture；PolyP bacteria kind daughter bacteria
Mass ratio with fluid nutrient medium is 10:100.Fluid nutrient medium is 3~5 parts of peptone, and beef leaches 3~5 parts of object, di(2-ethylhexyl)phosphate
800~1000 parts of hydrogen potassium buffer solution, 12~15 parts of agar；Phosphate buffer pH is 7.3.
Liquid acid-producing bacteria follows the steps below:
Acid-producing bacteria kind daughter bacteria is seeded in fluid nutrient medium and cultivates a period of time to get liquid acid-producing bacteria；
The pH of the fluid nutrient medium is 6.8；The incubation time is for 24 hours；Culture is Anaerobic culturel；Acid-producing bacteria kind daughter bacteria and liquid
The mass ratio of body culture medium is 10:100.Fluid nutrient medium be 3~5 parts of corn flour, 0.1~0.3 part of peptone, glucose 1~2
Part part, 12~15 parts of agar, distilled water 1L.
The polyP bacteria buying is in Chinese industrial Microbiological Culture Collection administrative center number CICC 22102.
The acid-producing bacteria buying is in Chinese industrial Microbiological Culture Collection administrative center number CICC 10350.
Phytase and calcium formate are added after liquid polyP bacteria and liquid acid-producing bacteria are mixed to get feed addictive.
Embodiment 15
Feed addictive is the composition of calcium formate, sodium Diacetate, white vitriol and polyP bacteria according to mass ratio 1:3:1:2；
The polyP bacteria is cultivated according to the following steps:
PolyP bacteria kind daughter bacteria is seeded in fluid nutrient medium and cultivates a period of time to get liquid polyP bacteria, by liquid polyP bacteria
After drying, solid polyP bacteria is obtained.
The pH of the fluid nutrient medium is 7.4；The incubation time is 30h；Culture is aerobic culture；PolyP bacteria kind daughter bacteria
Mass ratio with fluid nutrient medium is 10:100.Fluid nutrient medium is 5 parts of peptone, and beef leaches 3 parts of object, and potassium dihydrogen phosphate is slow
Rush 1000 parts of solution, 12 parts of agar；Phosphate buffer pH is 7.4；Drying temperature is 50 DEG C.
Calcium formate, sodium Diacetate, white vitriol and polyP bacteria are mixed, to get feed addictive after packaging.
Embodiment 16
Feed addictive contains polyP bacteria；
The polyP bacteria and its product are to animal nonhazardous.
The preparation method of feed addictive, to get feed addictive after polyP bacteria is packed；Or
PolyP bacteria and other feed addictives are mixed to get feed addictive.
Embodiment 17
Feed addictive contains polyP bacteria；
The polyP bacteria and its product are to animal nonhazardous.
Contain phosphorus in the polyP bacteria culture medium；
The preparation method of feed addictive mixes polyP bacteria and other feed addictives to get feed addictive；PolyP bacteria and
The mass ratio of other feed addictives is 1:8.
Other feed addictives are bile acid, calcium formate, sodium Diacetate, white vitriol, vitamin B according to quality
Composition than 2:1:2:3:1.
Embodiment 18
Feed addictive contains polyP bacteria；
The polyP bacteria and its product are to animal nonhazardous；
Contain sodium pyrophosphate in the polyP bacteria culture medium.
The preparation method of feed addictive, to get feed addictive after polyP bacteria is packed；Or
PolyP bacteria and other feed addictives are mixed to get feed addictive.
Above-mentioned phosphate buffer solution configuration:
PH7.0: taking potassium dihydrogen phosphate 6.8g, adds 0.1mol/L sodium hydroxide solution 291ml, be diluted with water to 1000ml to get；
PH7.2: taking 0.2mol/L potassium dihydrogen phosphate 250ml and 0.2mol/L sodium hydroxide solution 175ml, adds and newly boiled
Cold water is diluted to 1000ml, shakes up to obtain the final product；
PH7.3: taking disodium hydrogen phosphate 1.9734g and potassium dihydrogen phosphate 0.2245g, and water is added to make to be dissolved into 1000ml, adjust pH to
7.3 to get；
PH7.4: taking potassium dihydrogen phosphate 6.8g, adds 0.1mol/L sodium hydroxide solution 395ml, be diluted with water to 1000ml to get.
Beneficial effects of the present invention are further illustrated below with reference to experimental data:
Experiment one
1 materials and methods:
1.1 test sites: Yantai City's Long Island；
1.2 experiment detections: rate of body weight gain, the death rate, coefficient of condition, dirty body ratio, rouge body ratio；Total bile acid, third are detected after experiment
Dialdehyde, total antioxidant capacity and total superoxide dismutase；
1.3 materials to be tested: the premix of embodiment 1, comparison 1(by embodiment 1 premix and 700ppm bile acid form),
Embodiment 1 and comparison 2(when polyP bacteria culture in culture medium phosphate buffer solution with water substitution in addition to, other proportions and implementation
Example 1 is consistent)；
1 premix of embodiment matches 60 parts of dregs of beans, 10 parts of bone meal, 15 parts of fish meal, 5 parts of shrimp med, 3 parts of fish oil, 6 parts of corn flour, chlorine
0.1 part of choline of change, 0.3 part of multi-vitamins, 0.2 part of compound amino acid and 0.4 part of berberine；
1.4 experimental methods: by fish pond enclosure similar in primary condition at 6 50 square metres of pulk, every two pulk is one group,
200 snakeheaded fish are put on every pool in a suitable place to breed, after fry is put in a suitable place to breed, feed for early 8 points to every group of pulk every other day, feed 1kg, experimental period every time
It is 12 weeks；
1.5 detection methods: rate of body weight gain (%)=(last weight-initial weight)/initial weight × 100；
Coefficient of condition=100 × fresh fish weight (gram)/body is long3cm3；
Dirty body ratio=100 × internal organ weight/weight；
Liver body ratio=100 × liver weight/weight；
Tripe tallow ratio=100 × tripe tallow weight/weight；
Detect total bile acid, malonaldehyde, total antioxidant capacity and total superoxide dismutase in blood.
In addition to detection substance is different, other detection modes are consistent for this experiment
2 results and analysis
Rate of body weight gain, the death rate, coefficient of condition, dirty body ratio, rouge body ratio；Total bile acid, malonaldehyde, total antioxidation are detected after experiment
Ability and total superoxide dismutase testing result, are shown in Table 1
Although the comparison 1 that bile acid is added it can be seen from total bile acid detection can stimulate the growth of snakeheaded fish, 12 are fed
Zhou Hou, total bile acid too high levels in snakeheaded fish body, has had the tendency that hepatitis and cirrhosis, and bile acid and polyP bacteria is added
Embodiment 1 is then relatively healthy, and either weight gain or its intracorporal anti-oxidant degree are improved, and illustrates its vitality more
It is vigorous；Although comparison 2 joined polyP bacteria, phosphate buffer is free of in its culture medium, it can be seen from 1 data of table
Its data is significantly worse than the embodiment 1 in culture medium containing phosphate buffer better than comparison 1, it is seen that the polyP bacteria of addition
Culture medium in containing phosphorus be also the application key.
Experiment two
1 materials and methods:
1.1 test sites: Zhaoyuan City, the village Li Jiatan, Mt. Qishan town chicken-raising shed；
1.2 experiment detections: average egg weight, laying rate, broken (soft) egg rate；Detect total protein, total cholesterol, glycerol in Laying-hen Serum
Three esters and total bile acid；
1.3 materials to be tested: 2 premix of embodiment, comparison 3(by embodiment 2 premix and 700ppm bile acid form), it is real
Applying example 2 and comparison 4(, phosphate buffer solution is outer with water substitution in culture medium when polyP bacteria culture, other proportions and embodiment 2
It is consistent)；
2 premix of embodiment proportion, 65 parts of corn, 15 parts of dregs of beans, 5 parts of stone grain, cotton dregs 10 divide, 3 parts of hydrolyzed feather meal, phosphoric acid hydrogen
1 part of calcium, 0.8 part of rice bran oil, 0.15 part of lysine, 0.15 part of choline chloride, 0.3 part of multi-vitamins, 0.1 part of microelement,
0.2 part and 0.1 part of sodium bicarbonate of compound amino acid；
1.4 experimental methods: being randomly divided into 3 groups for the blue brown laying hen in sea that 180 have just hatched, and every group 60, using selected by experiment
Feed respectively feeds extra large blue brown laying hen, feeds to 400 days, and experiment continuously collects 3 days eggs before terminating, and every group every
It collects 20, statistical data.
In addition to detection substance is different, other detection modes are consistent for this experiment
2 results and analysis
Average egg weight, laying rate, broken (soft) egg rate；Detect total protein, total cholesterol, triglycerides and total bile in Laying-hen Serum
Sour testing result, is shown in Table 2
The laying rate of laying hen can be improved in 3 addition bile acid of comparison it can be seen from total bile acid detection, but total in laying hen body
Determination of Bile Acids is higher, and then total bile acid content is relatively low for the comparison 4 that bile acid and polyP bacteria is added and embodiment 2, and
The comparison 3 of bile acid is added and the comparison 4 of bile acid and polyP bacteria and embodiment 2 is added can reduce compared with normal diet
The content of triglycerides and blood glucose in chicken blood；Although comparison 4 joined polyP bacteria, phosphate-containing is not slow in its culture medium
Fliud flushing, its data is better than comparison 3 it can be seen from 2 data of table, but is significantly worse than the reality in culture medium containing phosphate buffer
Apply example 2, it is seen that be also the key of the application containing phosphorus in the culture medium of the polyP bacteria of addition.
Claims (10)
1. application of the polyP bacteria in feed.
2. a kind of feed addictive, which is characterized in that include polyP bacteria.
3. feed addictive as claimed in claim 2, which is characterized in that also containing one of acid-producing bacteria, organic acid and enzyme or
Two kinds of arbitrary proportion and its more than；
The acid-producing bacteria includes the two or more of one of lactic acid bacteria, clostridium, saccharomycete and bacillus or arbitrary proportion；Institute
Belong to enzyme include two kinds of one of phytase, cellulase, protease and 7 α hydroxylases or arbitrary proportion and its more than；It is described
Organic acid include two kinds of one of bile acid, amino acid, lactic acid and malic acid or arbitrary proportion and its more than.
4. the feed addictive as described in Claims 2 or 3, which is characterized in that the polyP bacteria is cultivated according to the following steps:
PolyP bacteria kind daughter bacteria is seeded in fluid nutrient medium and cultivates a period of time to get liquid polyP bacteria；Or by the poly- phosphorus of liquid
After bacterium is dry, solid polyP bacteria is obtained；
The pH of the fluid nutrient medium is 7.0~7.4；The incubation time is 20~48h；Culture is aerobic culture；PolyP bacteria
The mass ratio of kind daughter bacteria and fluid nutrient medium is 1~10:100；The drying temperature is 30~80 DEG C；
Contain phosphorus in the fluid nutrient medium；
The phosphorus is phosphate buffer, and phosphate buffer pH is 7.0~7.4.
5. feed addictive as claimed in claim 4, which is characterized in that fluid nutrient medium is 3~5 parts of peptone, beef leaching
3~5 parts of object, 800~1000 parts of potassium dihydrogen phosphate buffer solution, 12~15 parts of agar.
6. a kind of feed, which is characterized in that include polyP bacteria.
7. feed as claimed in claim 6, which is characterized in that also containing one of acid-producing bacteria, organic acid and enzyme or any ratio
Example two kinds and its more than；
The acid-producing bacteria includes the two or more of one of lactic acid bacteria, clostridium, saccharomycete and bacillus or arbitrary proportion；Institute
Belong to enzyme include two kinds of one of phytase, cellulase, protease and 7 α hydroxylases or arbitrary proportion and its more than；It is described
Organic acid include two kinds of one of bile acid, amino acid, lactic acid and malic acid or arbitrary proportion and its more than；
The mass ratio of remaining component of the quality and feed of the polyP bacteria is 0.1~4:1000.
8. feed as claimed in claims 6 or 7, which is characterized in that the polyP bacteria is cultivated according to the following steps:
PolyP bacteria kind daughter bacteria is seeded in fluid nutrient medium and cultivates a period of time to get liquid polyP bacteria；Or by the poly- phosphorus of liquid
After bacterium is dry, solid polyP bacteria is obtained；
The pH of the fluid nutrient medium is 7.0~7.4；The incubation time is 20~48h；Culture is aerobic culture；PolyP bacteria
The mass ratio of kind daughter bacteria and fluid nutrient medium is 1~10:100；The drying temperature is 30~80 DEG C；
Contain phosphorus in the fluid nutrient medium；
The phosphorus is phosphate buffer, and phosphate buffer pH is 7.0~7.4.
9. feed as claimed in claim 8, which is characterized in that fluid nutrient medium is 3~5 parts of peptone, and beef leaches object 3~5
Part, 800~1000 parts of potassium dihydrogen phosphate buffer solution, 12~15 parts of agar.
10. a kind of preparation method of feed, which is characterized in that by the feed addictive be added in feed pre-mixing material to get
The feed；Feed addictive described in 0.1kg~4kg is added in feed per ton.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910281124.4A CN109757608A (en) | 2019-04-09 | 2019-04-09 | The preparation method of application and additive, feed and feed of the polyP bacteria in feed |
| CN201910477925.8A CN110178974B (en) | 2019-04-09 | 2019-06-03 | Feed additive, feed and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910281124.4A CN109757608A (en) | 2019-04-09 | 2019-04-09 | The preparation method of application and additive, feed and feed of the polyP bacteria in feed |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109757608A true CN109757608A (en) | 2019-05-17 |
Family
ID=66460274
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910281124.4A Pending CN109757608A (en) | 2019-04-09 | 2019-04-09 | The preparation method of application and additive, feed and feed of the polyP bacteria in feed |
| CN201910477925.8A Active CN110178974B (en) | 2019-04-09 | 2019-06-03 | Feed additive, feed and preparation method thereof |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910477925.8A Active CN110178974B (en) | 2019-04-09 | 2019-06-03 | Feed additive, feed and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN109757608A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109820876A (en) * | 2019-04-10 | 2019-05-31 | 晟丰(烟台)农业科技有限公司 | Biological medicine and its preparation method and application |
| CN110178974A (en) * | 2019-04-09 | 2019-08-30 | 晟丰(烟台)农业科技有限公司 | Feed addictive, feed and preparation method thereof |
| CN111771813A (en) * | 2019-04-03 | 2020-10-16 | 无锡三智生物科技有限公司 | Healthy breeding method for laying hens |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105876133A (en) * | 2016-04-13 | 2016-08-24 | 江苏星海生物科技有限公司 | Feed additive for aquatic organisms and preparation method thereof |
| CN106408114A (en) * | 2016-08-31 | 2017-02-15 | 东北农业大学 | Application of multivariate linear regression model to predicting chicken abdominal fat quantity or breeding low fat chicken |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107467413A (en) * | 2017-07-31 | 2017-12-15 | 兰溪市酉泽饲料技术服务有限公司 | The preparation method of loach scrod feed |
| CN109393250A (en) * | 2018-12-20 | 2019-03-01 | 储福娣 | A kind of salmon feed production method |
| CN109757608A (en) * | 2019-04-09 | 2019-05-17 | 晟丰(烟台)农业科技有限公司 | The preparation method of application and additive, feed and feed of the polyP bacteria in feed |
-
2019
- 2019-04-09 CN CN201910281124.4A patent/CN109757608A/en active Pending
- 2019-06-03 CN CN201910477925.8A patent/CN110178974B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105876133A (en) * | 2016-04-13 | 2016-08-24 | 江苏星海生物科技有限公司 | Feed additive for aquatic organisms and preparation method thereof |
| CN106408114A (en) * | 2016-08-31 | 2017-02-15 | 东北农业大学 | Application of multivariate linear regression model to predicting chicken abdominal fat quantity or breeding low fat chicken |
Non-Patent Citations (6)
| Title |
|---|
| 何杰: "饲料添加胆汁酸对异育银鲫生长、氧化损伤修复、内源胆汁酸代谢以及 CyHV-2 免疫保护作用的影响[D]", 《苏州大学》 * |
| 刘伟等: "盐度对大麻哈鱼幼鱼血液生化指标及肝组织的影响", 《应用生态学报》 * |
| 周华金等: "热应激对肉仔鸡肝AMPK_1及脂肪代谢相关分子基因表达的影响", 《畜牧兽医学报》 * |
| 左旭冬等: "饲粮中添加鹅脱氧胆汁酸对种鸡脂代谢、生产性能及子代肌肉发育的影响", 《动物营养学报》 * |
| 赵丽红等主编: "《水处理生物学》", 31 October 2015, 东北大学出版社 * |
| 郭斌等: "江蓠、浒苔、藻渣和菌渣替代鱼粉对大菱鲆幼鱼生长性能、血清和肝脏生化指标、体组成和肠道组织结构的影响", 《动物营养学报》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111771813A (en) * | 2019-04-03 | 2020-10-16 | 无锡三智生物科技有限公司 | Healthy breeding method for laying hens |
| CN110178974A (en) * | 2019-04-09 | 2019-08-30 | 晟丰(烟台)农业科技有限公司 | Feed addictive, feed and preparation method thereof |
| CN110178974B (en) * | 2019-04-09 | 2021-09-07 | 晟丰(烟台)农业科技有限公司 | Feed additive, feed and preparation method thereof |
| CN109820876A (en) * | 2019-04-10 | 2019-05-31 | 晟丰(烟台)农业科技有限公司 | Biological medicine and its preparation method and application |
| CN110179832A (en) * | 2019-04-10 | 2019-08-30 | 晟丰(烟台)农业科技有限公司 | Biological medicine and its preparation method and application |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110178974A (en) | 2019-08-30 |
| CN110178974B (en) | 2021-09-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zhou et al. | Enhancement of growth and intestinal flora in grass carp: the effect of exogenous cellulase | |
| CN107815429B (en) | Granulation-resistant lactobacillus preparation and preparation method thereof | |
| Liang et al. | Application of enzymes as a feed additive in aquaculture | |
| UA125639C2 (en) | Feed additive composition | |
| JP2014508519A (en) | Composition for feed addition | |
| CN111034878A (en) | Compound feed additive for improving growth performance of aquatic animals and preparation method thereof | |
| TW201616976A (en) | Direct-fed microbials and methods of their use | |
| JP2014507946A (en) | Composition for feed addition | |
| JP2002527046A (en) | Antimicrobial enzymes in animal feed | |
| EP2371226B1 (en) | Bile salt adjuvant for animals for improving fat utilization efficiency in the bodies of animals | |
| CN109757608A (en) | The preparation method of application and additive, feed and feed of the polyP bacteria in feed | |
| CN101797016A (en) | Animal growth modulating agent and preparation method thereof | |
| CN108967742A (en) | A kind of compound micro-ecological preparation used for aquiculture and feed | |
| Wang et al. | Improving the quality of Laminaria japonica-based diet for Apostichopus japonicus through degradation of its algin content with Bacillus amyloliquefaciens WB1 | |
| Raghuvansi et al. | Effect of complete feed blocks or grazing and supplementation of lambs on performance, nutrient utilisation, rumen fermentation and rumen microbial enzymes | |
| CN114747688A (en) | 'four-in-one' combined additive capable of improving intestinal functions of dogs and cats and preparation method thereof | |
| Lamid et al. | Potential of phytase enzymes as biocatalysts for improved nutritional value of rice bran for broiler feed | |
| Ranjan et al. | Strategies for maximizing utilization of de-oiled rice bran (DORB) in the fish feed | |
| BR112020015899A2 (en) | COMPOSITIONS, AND METHOD FOR PREPARING FERMENTED COMPOSITION WITH IMPROVED ODOR USING YEAST | |
| JP2018509161A (en) | Archaea in bioactive animal feed, method for producing the composition, and method using the composition | |
| US20230059825A1 (en) | Bacillus strains with the ability to degrade inorganic nitrogen compounds | |
| Belal | Assessment of the performance of chicks fed with wheat bran solid fermented by Trichoderma longibrachiatum (SF1) | |
| CN109722392B (en) | Culture medium capable of promoting bacillus subtilis to produce spores and application thereof | |
| Azari et al. | The effects of commercial probiotic and prebiotic usage on growth performance, body composition and digestive enzyme activities in juvenile rainbow trout (Oncorhynchus mykiss) | |
| CN108783075A (en) | Improve the complex enzyme formulation of meat chicken production performance and intestine microenvironment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190517 |
|
| WD01 | Invention patent application deemed withdrawn after publication |