CN109757518A - A kind of composition pesticide preparation method and application containing polyoxin B, bacillus subtilis - Google Patents
A kind of composition pesticide preparation method and application containing polyoxin B, bacillus subtilis Download PDFInfo
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- CN109757518A CN109757518A CN201711096406.4A CN201711096406A CN109757518A CN 109757518 A CN109757518 A CN 109757518A CN 201711096406 A CN201711096406 A CN 201711096406A CN 109757518 A CN109757518 A CN 109757518A
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- bacillus subtilis
- polyoxin
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Abstract
It is to pass through a kind of compound that fermentating metabolism generates by actinomyces that the invention discloses a kind of containing antimycin B, the composition of bacillus subtilis and its application in pesticide, polyoxin B, in the prevention and treatment for being agriculturally mainly used in controlling fungal diseases of crop, bacillus subtilis be also by fermentation, concentration, efficient centrifugal spray drying and produce brood-gemma agriculture body, the bacillus prevention and treatment rice, banded sclerotial blight, tobacco bacterial wilt, bacterial disease, significant effect.Discovery is tried by field, the two can not only promote drug effect after mixing, while can also prevent and treat new disease, and effectively reducing the two single dose usage amount reduces use cost, while can reduce residual agriculture, raising crop quality, guarantee food safety with substituted chemistry reagent.
Description
Technical field
The present invention relates to biological pesticide technical fields, and in particular to the production etc. to polyoxin, bacillus subtilis is raw
Object technical field.
Background technique
Polyoxin (polyoxin) is wide spectrum medicine antibiotics, is a kind of active matter generated by actinomyces through everfermentation
Matter, having absorption conduction, its mechanism of action in preferable is the biosynthesis for interfering cell wall chitin, germ tube and mycelia
Body contacts local bulkiness, alkali cracking, spilling cell inclusion after medicament, and is unable to normal development, dead.Also having inhibits germ to produce
The expansion effect of raw spore and scab, be mainly used for preventing and treating black spot common on the industrial crops such as melon fruits and vegetables, powdery mildew,
A variety of interior fungus-caused diseases such as damping-off, wilt disease, while to the banded sclerotial blight of rice, wheat powdery mildew, red bacterium disease have bright
Aobvious control efficiency.And promote the growth of crop, wide spectrum low toxicity, low-residual, to dynamic preferable with adaptability naturally and compatibility
Object does not have toxicity, does not have phytotoxicity to plant.
Bacillus subtilis is one kind of bacillus, 0.7-0.8 × 2-3 microns of individual cells, uniform coloring, and nothing
Pod membrane, peritrichous can move.Gram-negative bacteria, 0.6-0.9 × 1.0-1.5 microns of gemma oval to column, is located at thallus
Central or slightly inclined, thallus does not expand after sporulation, and bacterium colony rough surface is opaque, dirty white, it is grown numerous by competitiveness
It grows, occupies the mode of living space to prevent the growth of phytopathogen, layer protecting film can be formed in plant surface, make crop
From disease infestation, in addition bacillus subtilis can also secrete antibacterial substance, inhibit germ spore development and mycelia growth, simultaneously
A variety of enzymes decomposition, degradation soil with organic matter and inanimate matter can also be secreted, nutrition needed for plant is provided.
Polyoxin is in the nature the antibiotic of Mycophyta, and to bacterium unrestraint effect, bacillus subtilis is bacterium, the two
After being mutually mixed, be independent of each other use, and can increase drug effect, generates the effect that one-plus-one is greater than two.
Summary of the invention
The purpose of the present invention is being that developing a drug effect cooperates with increased biological pesticide formula, benefit increases anti-with synergistic effect
Effect, reduces the disease of crops, the purpose increased production, increased income.
Polyoxin of the present invention is to contain cornstarch 3.5%, dregs of beans in affiliated culture medium using streptomyces aureus
Powder 3%, yeast powder 0.5%, mannitol 0.5%, salt 0.5%, ammonium sulfate 0.05%, calcium carbonate 0.2%, are cultivated under 28 DEG C of condition of culture
3 days, the cleaner liquid of fermentation liquid is obtained after the thallus of fermentation liquid is filtered, is spray-dried to obtain polyoxin production through efficient centrifugal
Product.
The bioactivity of the fermentation liquid of above method preparation is 32000IU/ml, and the biological effect of original powder is 700000IU/
Ml uses the red germ of tobacco as indicator bacteria using cylinder plate method.
Bacillus subtilis of the present invention be CGMCC using number, NO:9269 in the culture medium, starch 1%,
Glucose 0.1%, groundnut meal 2%, fish meal 0.5%, potassium dihydrogen phosphate 0.04%, magnesium sulfate 0.075%, under 30 DEG C of condition of culture
Culture 16h obtains the fermentation liquid of hundred million CFU/mL living spores.200,000,000,000 CFU/g are dried to obtain by ceramic membrane concentration and efficient spray
The bacillus subtilis original powder of living spores.
The effective content analysis method of the bacillus subtilis uses dilution-plate method, and the bacteria suspension after dilution divides
0.1mL is not taken to be uniformly coated in ready counting plate, culture for 24 hours, measures single colonie quantity, with analytical unit weight
In living spores number.
Preferably, above-mentioned biological pesticide is wettable powder, and the content of each ingredient is polyoxin 10%, withered grass
Bacillus 50,000,000,000 CFU/g, auxiliary agent 45-10% fill auxiliary material surplus.
The auxiliary agent is wetting agent, dispersing agent, stabilizer, synergist, any two or more, the filling in bleeding agent
Auxiliary material is one or more of diatomite, kaolin, clay.
Preferably, in above-mentioned biological pesticide, the dispersing agent be dodecyl sodium sulfate, sodium lignin sulfonate,
Butyl tea sodium sulfonate or dispersing agent NNO, the bleeding agent are bleeding agent JFO.
Preferably, the percentage composition of each composition quality is 200,000,000,000/g bacillus subtilis in above-mentioned biological pesticide
25%-30%, 70% polyoxin raw medicine 15%-17%, auxiliary agent 6%-9%, wherein dispersing agent NNO 5%, dodecyl sodium sulfate 3%-
6%, diatomite, clay surplus.
Preferably, the biological pesticide is for preventing and treating rice disease, such as rice sheath blight disease.
The present invention also provides polyoxin bioactivity detection method and the measuring methods of bacillus subtilis.
The present invention has the following effects compared with prior art
The present invention effectively compounds microbial bacterial agent (bacillus subtilis) agricultural antibiotic fungicide (polyoxin), by 10%
Polyoxin be added 50,000,000,000 CFU/g bacillus subtilis, after mixing intend with find the mixture be not only able to raising original deposit it is anti-
Steady seasonal febrile diseases, the drug effect of rice sheath blight disease are controlled, and new disease can be prevented and treated, changes the not good enough status of the drug effect of single dose, effectively
The usage amount of chemical pesticide is reduced, pesticide residue is reduced, ensure that food safety.
Specific embodiment
Below with reference to Concrete facts example, the invention will be further described, to help the contents of the present invention are understood.
Example of formulations one
Polyoxin is polyoxin raw medicine (polyoxin content is that 60%-70% is not diluted), and bacillus subtilis original powder is
200000000000 CFU/g living spores.
A kind of wettable powder is made of the raw material of following percentage: polyoxin raw medicine 15%-17%, bacillus subtilis
Bacterium original powder 25%-30%, auxiliary agent 6%-10%, surplus fill auxiliary material.
Auxiliary agent is wetting agent, dispersing agent, stabilizer, synergist, two or more in bleeding agent；Filling auxiliary material is diatom
One or more of soil, kaolin, clay.
Above-mentioned raw materials after the mixing of bipyramid ribbon mixer, through air-flow crushing obtain fineness be 45 μm of powder below at
Product.
Example of formulations two
The fermentation liquid for leading to aerobe fermentation by streptomyces aureus concentrates the filtrate to 1/10 by micro-filtration, collection filtrate.
The fermentation liquid obtained by fermentation of bacillus subtilis, effective gemma are 20,000,000,000 CFU/ml.It is concentrated, is abandoned by ceramic membrane
Cleaner liquid is removed, concentrate is collected in concentration 1/7.
The concentrate of the two is mixed, auxiliary agent wetting agent, dispersing agent, stabilizer, two or more in synergist are added,
Filling auxiliary material is diatomite, is uniformly mixed.
Mixed liquor is spray-dried by efficient centrifugal, obtaining content is 10% polyoxin, the withered grass gemma of 50,000,000,000 CFU/g
Bacillus, mixing formula finished product.
In order to facilitate above-mentioned technical proposal of the invention is understood, below by way of specific example, to above-mentioned technology of the invention
Scheme is described in detail, following embodiment be medicine component in be parts by weight, bacillus subtilis living bacteria count 500
Hundred million/g.
Table 1: embodiment component ratio
Preventive effect embodiment
Preventive effect embodiment one:
The wettable powder of the embodiment and comparative example 1-4 of above-mentioned preparation is diluted with water, is applied to the banded sclerotial blight of rice by spraying
Prevention and treatment, medicament fountain height are 100g/ mus, as a result preventive effect table
Table 2: rice sheath blight disease controlling effect
Data analyze the mixing formula of embodiment 1, and usage amount is the half of single dose usage amount, but preventive effect medicine is better than single dose.
Preventive effect embodiment two:
The wettable powder of the embodiment and comparative example 1-4 of above-mentioned preparation is diluted with water, by spraying applied to Chinese cabbage root rot
Prevention and treatment, control efficiency are shown in Table
Table 3: Chinese cabbage root rot preventive effect
Chinese cabbage root rot be by bacterium, fungi jointly caused by Chinese cabbage disease, statistics indicate that single dose control efficiency is not good enough, embodiment
After one pesticide control application, control efficiency is significant, and better than the chemical reagent of control column.
Claims (7)
1. a kind of composition containing polyoxin B, bacillus subtilis.
2. preparing the application in pesticide containing polyoxin B, bacillus subtilis bacteria composition described in claim 1.
3. application according to claim 2, it is characterised in that the product can be applicable to disease caused by crop fungi, bacterium
Evil.
4. a kind of pesticide contains polyoxin B, bacillus subtilis, auxiliary agent and filling auxiliary material.
5. according to claim 4 pesticide it is characterized in that, the polyoxin content be 10%, bacillus subtilis bacterial content
In 50,000,000,000 CFU/g living spores.
6. pesticide according to claim 4, which is characterized in that it is wettable powder, it is former using 70000IU/g polyoxin
Medicine, 200,000,000,000 CFU/g bacillus subtilis raw medicines, auxiliary agent are wetting agent, dispersing agent, stabilizer, synergist, appointing in bleeding agent
It anticipates two or more, the filling auxiliary material is one or more of diatomite, kaolin, clay, mixes in proportion, obtains right
Active constituent content in 5.
7. after being mixed according to product described in right 6 by raw material, air-flow crushing mixing, and needed for being obtained by centrifugal spray drying
Content, two methods prepare wettable powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711096406.4A CN109757518A (en) | 2017-11-09 | 2017-11-09 | A kind of composition pesticide preparation method and application containing polyoxin B, bacillus subtilis |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711096406.4A CN109757518A (en) | 2017-11-09 | 2017-11-09 | A kind of composition pesticide preparation method and application containing polyoxin B, bacillus subtilis |
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| Publication Number | Publication Date |
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| CN109757518A true CN109757518A (en) | 2019-05-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201711096406.4A Pending CN109757518A (en) | 2017-11-09 | 2017-11-09 | A kind of composition pesticide preparation method and application containing polyoxin B, bacillus subtilis |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110373363A (en) * | 2019-08-22 | 2019-10-25 | 福建三炬生物科技股份有限公司 | A kind of concentration microbial inoculum of bacillusmusilaginosiengineering and its preparation method and application |
-
2017
- 2017-11-09 CN CN201711096406.4A patent/CN109757518A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110373363A (en) * | 2019-08-22 | 2019-10-25 | 福建三炬生物科技股份有限公司 | A kind of concentration microbial inoculum of bacillusmusilaginosiengineering and its preparation method and application |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190517 |
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| WD01 | Invention patent application deemed withdrawn after publication |