CN109752532B - Incubation sample loading device based on multi-channel immunochromatography detection - Google Patents
Incubation sample loading device based on multi-channel immunochromatography detection Download PDFInfo
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Abstract
The invention discloses a multi-channel immunochromatography detection-based incubation sample loading device, and aims to realize multi-channel incubation and simultaneous sample loading. The device comprises a top cover, a sealed detection column, a sample introduction cover plate, a sample introduction pipeline, a base and an incubation disc. The top cover is used for sealing the top end of the sealed detection column and providing a pressure environment required by uniform mixing and sample introduction; the sealed detection column is used for carrying out chromatographic reaction; the sample feeding cover plate is used for sealing the base; the sample introduction pipeline provides a sample introduction channel for incubating samples; the base is used for containing a sample loading solution; the incubation disc is used for containing the enzyme labeling holes to realize pre-incubation reaction. The invention can also be used for detecting items without a pre-incubation step, and the pressure change of the device in the environment with the required pressure change can be realized by a manual or automatic device, so that multi-channel incubation and synchronous sample introduction are realized. The method can be applied to fields including clinical diagnosis, inspection and quarantine, food safety detection, judicial identification and the like, and has wide market prospect.
Description
Technical Field
The invention relates to a detection device, in particular to an incubation sample loading device based on multi-channel immunochromatography detection, and belongs to the technical field of biomedicine.
Background
Chromatography is short for chromatography, and takes common immunochromatography as an example, the principle is that an antibody specific to an object to be detected is firstly fixed on a certain specific area of a nitrocellulose membrane, then a sample to be detected is dripped to one end of the membrane, the sample moves to the area fixed with the antibody under the action of capillary force, and when the two meet, an antigen to be detected in the sample can be specifically combined with the immobilized antibody. In this case, if the analyte is stained with an immunological nanoparticle such as colloidal gold or an immunoenzyme, or a marker molecule having raman, chemiluminescence, up-conversion luminescence, and a time-resolved fluorescence signal is bound thereto, qualitative and quantitative measurement of the analyte can be achieved.
Because the chromatographic detection method is widely applied in a plurality of fields such as medical treatment, food, medicine, chemistry and judicial identification, some chromatographic detection devices appear in the market at present, including the multifunctional high-flux automatic chromatographic detection instrument which appears recently, and the simultaneous incubation and sample loading before the multichannel measurement can not be realized for the index which needs the pre-incubation method to detect.
Disclosure of Invention
Aiming at the defects of the existing products, the invention discloses a incubating and sample loading device based on multi-channel immunochromatography detection, and aims to realize multi-channel incubation and simultaneous sample loading. In order to achieve the purpose, the invention adopts the following technical scheme:
a hatching sample loading device based on multi-channel immunochromatography detection is composed of a top cover, a sealed detection column, a sample loading cover plate, a sample loading pipeline, a base and a hatching disc. The top cover is used for sealing the top end of the sealed detection column and providing a pressure environment required by uniform mixing and sample introduction; the sealed detection column is used for carrying out chromatographic reaction; the sample feeding cover plate is used for sealing the base; the sample introduction pipeline provides a sample introduction channel for incubating samples; the base is used for containing a sample loading solution; the incubation disc is used for containing the enzyme labeling holes to realize pre-incubation reaction.
The number of the holes at the bottom of the top cover corresponds to the number of the holes at the top end of the detection column, and the center of the top cover is provided with a hole which can be communicated with an external device and can provide variable air pressure through a manual or automatic device; the sealed detection column is obtained by opening a hole on the side surface of a columnar polyhedral structure (patent number: 2016112523767) and sealing the hole with a hyaluronic material; the sample introduction cover plate is used for sealing the detection column base; the sample introduction pipeline provides a channel for the incubated sample to enter the base sample holding area from the enzyme labeling hole and a channel for uniformly mixing the incubation solution through positive and negative pressure change; the structure and the function of the base are described in detail in a corresponding patent (patent number: 2016112523767); the incubation disc is used for containing an enzyme-labeled hole containing a material to be incubated, and a sample to be detected in the enzyme-labeled hole can be added manually or automatically;
the incubation sample loading device based on the multichannel immunochromatography detection can be made of plastic or all plastic materials such as metal, paper, glass and the like.
The incubation sample loading device based on the multi-channel immunochromatography detection can realize multi-channel incubation and simultaneous sample loading aiming at multi-principle chromatography detection, and can reduce errors caused by complex incubation and sample injection links in multi-index simultaneous chromatography detection.
The invention can also be used for detection without a pre-incubation step, can be applied to the fields of clinical diagnosis, inspection and quarantine, food safety detection, judicial identification, drug detection and the like, and has wide market prospect.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings obtained from the drawings belong to the protection scope of the present invention for those skilled in the art without creative efforts.
FIG. 1 is an assembly diagram of a multi-channel immunochromatography detection-based incubation sample loading device according to the present invention
In the drawings, the components represented by the respective reference numerals are listed below:
1. a top cover; 2. a sealed detection column; 3. a sample introduction pipeline; 4. a sample feeding cover plate; 5. an incubation tray;
FIG. 2 is a schematic diagram of a multi-channel immunochromatography detection-based incubation sample loading device
In the drawings, the components represented by the respective reference numerals are listed below:
1.1, the top of the top cover; 1.2, the bottom of the top cover; 2. a sealed detection column; 3. a sample introduction pipeline; 4. a sample feeding cover plate; 5. a detection column base; 6. an incubation tray;
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1 incubation sample loading device based on multichannel immunochromatography detection for sample detection
The method comprises the following operation steps: placing an enzyme-labeled hole corresponding to an index to be detected in an incubation disc, placing corresponding immunochromatographic test paper in a sealed detection column, and covering the top of the column; adding 200 mul of solution to be detected into each enzyme-labeled hole, and incubating for 2 min; and thirdly, the injector is connected with the top opening of the top cover, and pressure change between the detection column and the enzyme labeling hole is realized through repeated suction, so that the full mixing of each incubation solution is realized. Pumping air again, sucking the incubation solution in each enzyme-labeled hole into the corresponding sample containing areas of the detection column base through negative pressure, and starting the immunochromatographic reaction on the chromatographic test paper based on capillary action; fourthly, after waiting for 5min, judging and reading the measuring result.
The structure and the application of the incubation sample loading device based on the multi-channel immunochromatography detection provided by the embodiment of the invention are described in detail above. The device can also be used for multi-channel simultaneous sample loading immunochromatography detection without pre-incubation indexes. The environment of the device with the required pressure change can be realized by manually drawing a container such as an injector, and the like, and can also be connected with an automatic pressure change device to achieve the pressure change of the whole device, thereby realizing the expected purpose. The device can be used together with a detection instrument, and the instrument can be used for carrying out quantitative or semi-quantitative automatic analysis on a sample by using detection means such as visible light, chemiluminescence, time-resolved fluorescence, up-conversion luminescence, Raman and the like. The target object detected by the device can be biomacromolecules such as protein, nucleic acid, sugar and the like, micromolecular compounds, cell and subcellular components and the like, and can be used in the fields of clinical diagnosis, inspection and quarantine, food safety detection, judicial identification, medicine, drug detection and the like.
The principles and embodiments of the present invention have been described herein using specific examples, which are provided only to help understand the method and the core concept of the present invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, there may be variations in the specific embodiments and the application scope, and in summary, the content of the present specification should not be construed as a limitation to the present invention.
Claims (1)
1. A kind of incubation sample loading device based on multi-channel immunochromatography detection, the device is composed of a top cover, a sealed detection column, a sample loading cover plate, a sample loading pipeline, a base and an incubation disc; the sealed detection column is of a polyhedral cylindrical structure, a plurality of openings are formed in the top end of the sealed detection column, a plurality of detection column channels are correspondingly formed in the top end of the sealed detection column, the base is located at the bottom of the sealed detection column, and a plurality of base sample containing areas corresponding to the channels in the sealed detection column are formed in the base; the top cover seals the top end of the sealed detection column, the top cover consists of the top cover and the bottom of the top cover, the number of holes in the bottom of the top cover corresponds to the number of holes in the top end of the detection column, the center of the top cover is provided with a hole, and the sample introduction cover plate seals the sample containing area of the base; a base is placed on the inner ring of the incubation disc, a plurality of enzyme-labeled holes with the number corresponding to that of the base sample containing area are arranged on the outer edge of the incubation disc, each enzyme-labeled hole is communicated with the base sample containing area through a sample introduction pipeline, one end of the sample introduction pipeline penetrates through the sample introduction cover plate, and the other end of the sample introduction pipeline extends into the enzyme-labeled hole;
the incubation sample loading device based on the multichannel immunochromatography detection is used for sample detection and comprises the following operation steps: placing an enzyme-labeled hole corresponding to an index to be detected into an incubation disc, placing corresponding immunochromatographic test paper into a detection column channel of a sealed detection column, and covering the top of the detection column with a cover; adding 200 mul of solution to be detected into each enzyme-labeled hole, and incubating for 2 min; connecting an injector through an opening at the top of the top cover, realizing pressure change between the detection column and the enzyme labeling hole by repeatedly pumping, further realizing full mixing of each incubation solution, pumping again, simultaneously sucking the incubation solution in each enzyme labeling hole into a sample containing area corresponding to each detection column base through negative pressure, and starting the immunochromatography reaction on the chromatography test paper based on capillary action; fourthly, after waiting for 5min, judging and reading the measuring result.
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