CN106680492A - Structure of integrated detecting device, preparation method and application - Google Patents
Structure of integrated detecting device, preparation method and application Download PDFInfo
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- CN106680492A CN106680492A CN201611252376.7A CN201611252376A CN106680492A CN 106680492 A CN106680492 A CN 106680492A CN 201611252376 A CN201611252376 A CN 201611252376A CN 106680492 A CN106680492 A CN 106680492A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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Abstract
The invention discloses a structure of an integrated detecting device, a preparation method and application. The device has a columnar polyhedral structure, a hole is formed in a side surface of the device, fixing assemblies can be arranged at two ends of the device, and the device can be provided with a cover or does not need the cover, and can be used horizontally, vertically or obliquely. Various test strips in different detecting principles can be fixed by separate or integrated clamping shells, and simultaneous rapid detection of single sample or multiple samples and single index or multiple indexes can be realized by an automatic, semi-automatic or manual sampling device. A detection result can be judged with naked eyes and can also be qualitatively, quantitatively or semi-quantitatively analyzed by a corresponding instrument spectrum detection means. Target objects detected by the device can be biomacromolecules such as nucleic acid, protein and saccharides, and can also be small molecule compound and various cell and subcellular fractions. The device is small in size, convenient to carry and simple to operate, and can be used for the fields of clinical diagnosis, inspection and quarantine, food safety detection, judicial expertise, illicit drugs inspection and the like.
Description
Technical field
The present invention relates to a kind of analytical equipment, belong to field of fast detection, and in particular to the structure of integrated detection means,
Preparation method and purposes, are capable of achieving quick detection while monocyte sample or Multi-example, single index or multi objective.
Background technology
Chromatography is that a kind of difference using determinand each component property is separated and method for measuring to it, and conventional has
Paper chromatography, thin-layer chromatography, silica gel column chromatography etc..Immuno-chromatographic test paper strip is most commonly that in field of fast detection, will sample
One end of test strips is added drop-wise to, sample is to be moved up along test strips, if containing composition to be measured in sample, the component will
With reference in the corresponding position of test strips, the difference according to test strips operation principle combines different detection means, such as visible ray,
The tester of Raman, chemiluminescence, the luminous, time-resolved fluorescence of upper conversion etc., you can realize surveying the qualitative, quantitative of determinand
It is fixed.
Tomographic results detection card common in the market is usually single channel detection product, including chromatographic test paper and plastics
Cartridge two parts, test strips are placed in inside plastics cartridge, have three to abuse in cartridge, are respectively used to point sample, result observation
With it is ventilative.And such it is designed with many weak points：(1) multiple indexs can not be carried out to a sample or multiple sample simultaneously
Detection, easily causes the waste of sample；(2) cannot be combined with follow-up detection means automatically, be realized quantitative determination.
The content of the invention
The present invention discloses a kind of structure, the preparation method of integrated detection means for the deficiency of above-mentioned existing product
And purposes, it is therefore intended that realize quick detection while to monocyte sample or Multi-example, single index or multi objective.It is above-mentioned in order to reach
Purpose, the present invention is adopted the following technical scheme that：
The invention discloses a kind of structure of integrated detection means, preparation method and purposes.Described device generally post
Shape multi-plane structure, including but not limited to inner prop and the two parts that get stuck, can there is lid also uncovered, and two can have fixation kit also not have
Have, with reference to the test strips of various different Cleaning Principles, coordinate automatic, semi-automatic or hand sampling device, be capable of achieving monocyte sample or
Quick detection while Multi-example, single index or multi objective.Testing result can visually judge also to coordinate corresponding instrument spectral
Means realize the analysis of qualitative quantitative or sxemiquantitative.
Further, described device lateral opening hole, two can have fixation kit also to can be without, and have lid or do not cover, level, perpendicular
Straight or inclination is used.
Used as the preferred technical solution of the present invention, device material can be all shapings such as plastics, metal, glass, paper
Material；Can be prepared using methods such as 3D printing, injection, casting, forging, welding, braiding, punching presses.Whole height is generally 25-
Between 200mm, inner prop and the height that gets stuck may be the same or different.
Specifically, the inner column diameter of apparatus of the present invention is changeable, usually 5-50mm, and the side wall of inner prop is interval
Cylindrical recesses structure (1), a length of 10-200mm, a width of 1-10mm, the quantity of groove is 1-24, preferably 4-12.Groove
Between gap (2) size for 3mm or so it is adjustable.The top and bottom of groove are provided with the aperture (3) for getting stuck fixed, hole
Shape, size are adjustable.Inner prop inside can be entity, structure that is hollow or having specific use, be determined on a case-by-case basis.
Additionally, each groove outside is got stuck with a strip, can independently or multiple is connected between getting stuck.When getting stuck
When separate, between 25-200mm, a width of 1-20mm, thickness is determined on a case-by-case basis the single length for getting stuck, generally 3mm
Left and right.The upper and lower sides for getting stuck are provided with and groove top and the supporting bulge-structure of bottom aperture (4).The top and middle part got stuck
Sample well (5) and result monitoring window (6) are not provided with, and the sample well and result detect that the shape size of window is adjustable.
When be connected with each other between single getting stuck be integrally formed when, the single shape and structure that gets stuck with described in claim 5, phase
Neighbour two is connected between getting stuck with rectangular piece (7).From beginning to end at two get stuck it is connectionless (8) each other.Also can be adjacent two
The individual cohesive position for getting stuck separately sets sample-adding platform, and sample can be assigned at each test strips point sample through being loaded after platform is added.
Further, the present apparatus can be used cooperatively with the test strip of different principle.Test strips can be by pasting or putting
Put or the mode such as backboard is fixed on inner prop, get stuck positioned at test strips outside.
Device integrally can routinely be packed and be also vacuum-packed using the packaging such as plastics, masking foil.
The object of present apparatus detection can be the large biological molecules such as nucleic acid, protein, carbohydrate, or small molecule
Compound and various kinds of cell and subcellular components.Apparatus of the present invention small volume, be easy to carry, it is simple to operate, can be used for clinical diagnosis,
The fields such as inspection and quarantine, food safety detection, judicial expertise, illicit drugs inspection, Pharmaceutical Analysis.
Compared with prior art, patent of the present invention provides a kind of vertical multi-channel high flux chromatographic apparatus, device tool
Have the following advantages：(1) it is capable of achieving quick detection while monocyte sample or Multi-example, single index or multi objective；(2) can connect automatically
Subsequent detection equipment realizes quantitative determination, and simple to operate, associative operation personnel are without Special Training.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
The accompanying drawing to be used needed for having technology description is briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, according to this
Other accompanying drawings that a little accompanying drawings are obtained belong to the scope of protection of the invention.
Fig. 1 is rod structure in apparatus of the present invention
Fig. 2 is the single structure that gets stuck of apparatus of the present invention
Fig. 3 is that apparatus of the present invention disjunctor gets stuck structure
Fig. 4 is apparatus of the present invention assembly structure
Specific embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is all other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
The embodiment of the present invention provides a kind of structure of integrated detection means, preparation method and purposes, is capable of achieving monocyte sample
Or quick detection while Multi-example, single index or multi objective.It is described in detail individually below.
Embodiment 1, the preparation of column chromatographic apparatus
Material requested：Upbox-3D printers one are purchased from Beijing Tiertime Technology Co., Ltd., raw material ABS trees
Fat, PLA are purchased from the enlightening Science and Technology Ltd. of Beijing three.
Operating procedure：1. structural model is designed by Auto CAD softwares according to actual needs；2. will design a model input
Upbox-3D printers carry out 3D printing.
Embodiment 2, the assembling of integrated detection means
Material requested：Inner prop (Fig. 1), it is single get stuck (Fig. 2), disjunctor gets stuck (Fig. 3), immuno-chromatographic test paper strip
Operating procedure：The assembling of the separate integrated detection means device that gets stuck：1. by sample detection test strips successively
It is bonded on the groove of inner prop (Fig. 1) (1)；2. single get stuck (Fig. 2) is buckled in test strips top, sample well (5) and result successively
Monitoring window (6) is respectively aligned to the relevant position of test strips.
The assembling of Multi-example Multichannel device：1. sample detection test strips are bonded at the groove (1) of inner prop (Fig. 1) successively
On；2. by disjunctor get stuck (Fig. 3) be buckled in test strips top, sample well and result monitoring window are respectively aligned to the corresponding positions of test strips
Put.
Embodiment 3, the detection of mark of hepatitis virus thing
Material requested：Inner prop (Fig. 1), it is single get stuck (Fig. 2), hepatitis b virus s antigen HBsAg test strips,
Anti-HBc HBcAb test strips, hepatitis B virus e antigen HBeAg test strips, hepatitis B
Viral e antibody HBeAb test strips, anti-HBs HBsAb test strips, hepatitis A virus IgM
Antibody test test strips, antibody of HCV test strip, hepatitis E virus IgM antibody test strip, the above
Test strips are purchased from Nantong Egens Biotechnology Co., Ltd..Testing sample is a human serum, is solved by Chinese people
Clinical laboratory of Fang Jun hospital generals provides.
Operating procedure：1. the step as described in embodiment 2 assembles chromatographic apparatus；Test strip is pasted onto column successively
On；2. testing sample is added by sample well (5)；3. 10min, observation experiment result are waited.
Experimental result：Hepatitis b virus s antigen HBsAg test strips, hepatitis B virus e antigen HBeAg inspections
Test paper slip and anti-HBc HBcAb test strips are presented two bright bands, in showing to treat test sample
Containing HBsAg, HBeAg and HBcAb, only C lines are presented red on remaining Test paper, show in sample without test other into
Point.
A kind of vertical multi-channel high flux chromatographic apparatus for being provided the embodiment of the present invention above are described in detail,
Quantitatively or semi-quantitatively analysis of the invention device to sample liquid can coordinate follow-up various different types for example visible ray, chemiluminescence, fluorescence,
The detecting instrument and analytic unit of Raman etc. are carried out.The object of apparatus of the present invention detection can be raw protein, nucleic acid, sugar etc.
Thing macromolecular, micromolecular compound and cell and subcellular component etc., can be used for clinical diagnosis, inspection and quarantine, food security inspection
The fields such as survey, judicial expertise, illicit drugs inspection.
Specific case used herein is set forth to principle of the invention and implementation method, and above example is said
It is bright to be only intended to help and understand the method for the present invention and its core concept；Originally simultaneously for those of ordinary skill in the art, according to
According to thought of the invention, will change in specific embodiments and applications, in sum, this specification content
Should not be construed as limiting the invention.
Claims (10)
1. the invention discloses a kind of structure of integrated detection means, preparation method and purposes.Described device generally column
Polyhedral structure, including but not limited to inner prop and the two parts that get stuck, can there is lid also uncovered, and two can have fixation kit also not have
Have, with reference to the test strips of various different Cleaning Principles, coordinate automatic, semi-automatic or hand sampling device, be capable of achieving monocyte sample or
Quick detection while Multi-example, single index or multi objective.Testing result can visually judge also to coordinate corresponding instrument to detect
Means realize the analysis of qualitative, quantitative or sxemiquantitative.
2. integrated detection means according to claim 1, it is characterised in that：Described device lateral opening hole, two can have
Fixation kit also be can be without, and has and covers or do not cover, and level, vertical or inclination are used.
3. the integrated detection means according to claim 1 and 2, it is characterised in that：Device whole height is generally 25-
Between 200mm, inner prop and the height that gets stuck may be the same or different.
4. integrated detection means according to claim 3, it is characterised in that：Inner column diameter is changeable, usually 5-
50mm, the side wall of inner prop is the cylindrical recesses structure at interval, a length of 10-200mm, a width of 1-10mm, and the quantity of groove is 1-24
It is individual, preferably 4-12.Gap length between groove is that 3mm or so is adjustable.The top and bottom of groove are provided with for getting stuck
Fixed aperture, the shape in hole, size are adjustable.Inner prop inside can be entity, structure that is hollow or having specific use, depending on
Depending on concrete condition.
5. integrated detection means according to claim 3, it is characterised in that：Match somebody with somebody a Strip-like clip in each groove outside
Shell, can independently or multiple is connected between getting stuck.When getting stuck separate, the single length for getting stuck 25-200mm it
Between, a width of 1-20mm, thickness is determined on a case-by-case basis, generally 3mm or so.The upper and lower sides for getting stuck are provided with and groove top and bottom
The supporting bulge-structure of end aperture.The top and middle part got stuck are respectively equipped with sample well and result monitoring window, the sample well and
The shape size of result detection window is adjustable.
6. integrated detection means according to claim 5, it is characterised in that：Turn into when being connected with each other between single getting stuck
During one, the single shape and structure that gets stuck with described in claim 5, adjacent two get stuck between be connected with rectangular piece.From beginning to end
Getting stuck at two is connectionless each other.Also sample-adding platform can be separately set in the two neighboring cohesive position for getting stuck, sample is through being loaded platform
Can be assigned at each test strips point sample after addition.
7. the integrated detection means according to claim 1 and 2, it is characterised in that：The present apparatus can be with the inspection of different principle
Test paper slip is used cooperatively.Test strips can be got stuck positioned at test paper by pasting or putting or the mode such as backboard is fixed on inner prop
Bar outside.
8. integrated detection means according to claim 1, it is characterised in that：Device can integrally use plastics, masking foil
Deng packaging, can routinely pack and also be vacuum-packed.
9. integrated detection means according to claim 1, it is characterised in that：Apparatus of the present invention material can be plastics, gold
All Plastic implants such as category, glass, paper, can be using methods such as 3D printing, injection, casting, forging, welding, braiding, punching presses
Prepare.
10. integrated detection means according to claim 1, it is characterised in that：The present apparatus detection object can be
The large biological molecules such as nucleic acid, protein, carbohydrate, or micromolecular compound and various kinds of cell and subcellular components.This hair
Bright device volume is small, be easy to carry, simple to operate, can be used for clinical diagnosis, inspection and quarantine, food safety detection, Pharmaceutical Analysis,
The fields such as judicial expertise, illicit drugs inspection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611252376.7A CN106680492A (en) | 2016-12-30 | 2016-12-30 | Structure of integrated detecting device, preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611252376.7A CN106680492A (en) | 2016-12-30 | 2016-12-30 | Structure of integrated detecting device, preparation method and application |
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| Publication Number | Publication Date |
|---|---|
| CN106680492A true CN106680492A (en) | 2017-05-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201611252376.7A Pending CN106680492A (en) | 2016-12-30 | 2016-12-30 | Structure of integrated detecting device, preparation method and application |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109752532A (en) * | 2017-11-03 | 2019-05-14 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | A kind of incubation sampling device based on the detection of multichannel immunochromatography |
| CN110275019A (en) * | 2019-08-01 | 2019-09-24 | 郑州迈迪迅医疗科技有限公司 | A kind of novel chromatography detection kit |
| CN110514819A (en) * | 2019-09-06 | 2019-11-29 | 安徽福贸生物科技有限公司 | A kind of detachable sample liquid detection device |
| CN113702562A (en) * | 2021-08-26 | 2021-11-26 | 浙江大学中原研究院 | Multi-mode integrated detector for chromatographic synchronous sample measurement and use method |
-
2016
- 2016-12-30 CN CN201611252376.7A patent/CN106680492A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109752532A (en) * | 2017-11-03 | 2019-05-14 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | A kind of incubation sampling device based on the detection of multichannel immunochromatography |
| CN109752532B (en) * | 2017-11-03 | 2022-04-15 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | Incubation sample loading device based on multi-channel immunochromatography detection |
| CN110275019A (en) * | 2019-08-01 | 2019-09-24 | 郑州迈迪迅医疗科技有限公司 | A kind of novel chromatography detection kit |
| CN110514819A (en) * | 2019-09-06 | 2019-11-29 | 安徽福贸生物科技有限公司 | A kind of detachable sample liquid detection device |
| CN113702562A (en) * | 2021-08-26 | 2021-11-26 | 浙江大学中原研究院 | Multi-mode integrated detector for chromatographic synchronous sample measurement and use method |
| CN113702562B (en) * | 2021-08-26 | 2023-11-14 | 浙江大学中原研究院 | Multi-mode integrated detector for chromatographic synchronous sample measurement and use method |
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