CN109735343B - Composition for accelerating degradation of pesticide residues in soil - Google Patents
Composition for accelerating degradation of pesticide residues in soil Download PDFInfo
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- CN109735343B CN109735343B CN201811590653.4A CN201811590653A CN109735343B CN 109735343 B CN109735343 B CN 109735343B CN 201811590653 A CN201811590653 A CN 201811590653A CN 109735343 B CN109735343 B CN 109735343B
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- cotton gauze
- degradation
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- humus
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- 238000006731 degradation reaction Methods 0.000 title claims abstract description 62
- 230000015556 catabolic process Effects 0.000 title claims abstract description 61
- 239000002689 soil Substances 0.000 title claims abstract description 53
- 239000000203 mixture Substances 0.000 title claims abstract description 28
- 239000000447 pesticide residue Substances 0.000 title claims abstract description 16
- 229920000742 Cotton Polymers 0.000 claims abstract description 93
- 241000894006 Bacteria Species 0.000 claims abstract description 38
- 239000003864 humus Substances 0.000 claims abstract description 37
- 230000000593 degrading effect Effects 0.000 claims abstract description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 15
- 238000007789 sealing Methods 0.000 claims abstract description 14
- 239000000575 pesticide Substances 0.000 claims description 10
- 239000000835 fiber Substances 0.000 claims description 8
- 239000007921 spray Substances 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 7
- 238000005507 spraying Methods 0.000 claims description 7
- 239000000126 substance Substances 0.000 claims description 6
- 239000012466 permeate Substances 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 241000736110 Sphingomonas paucimobilis Species 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- PUKLDDOGISCFCP-JSQCKWNTSA-N 21-Deoxycortisone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)C)(O)[C@@]1(C)CC2=O PUKLDDOGISCFCP-JSQCKWNTSA-N 0.000 claims description 3
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims description 3
- FCYKAQOGGFGCMD-UHFFFAOYSA-N Fulvic acid Natural products O1C2=CC(O)=C(O)C(C(O)=O)=C2C(=O)C2=C1CC(C)(O)OC2 FCYKAQOGGFGCMD-UHFFFAOYSA-N 0.000 claims description 3
- 230000009471 action Effects 0.000 claims description 3
- 239000002509 fulvic acid Substances 0.000 claims description 3
- 229940095100 fulvic acid Drugs 0.000 claims description 3
- 239000004021 humic acid Substances 0.000 claims description 3
- 239000002663 humin Substances 0.000 claims description 3
- 150000002894 organic compounds Chemical class 0.000 claims description 3
- 239000002344 surface layer Substances 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 2
- 239000004033 plastic Substances 0.000 claims description 2
- 238000004321 preservation Methods 0.000 claims description 2
- 239000012785 packaging film Substances 0.000 claims 1
- 229920006280 packaging film Polymers 0.000 claims 1
- 244000005700 microbiome Species 0.000 abstract description 6
- 238000003860 storage Methods 0.000 abstract description 4
- 238000003915 air pollution Methods 0.000 abstract description 2
- 241000233866 Fungi Species 0.000 abstract 3
- 238000010790 dilution Methods 0.000 abstract 1
- 239000012895 dilution Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 238000003900 soil pollution Methods 0.000 abstract 1
- 239000005946 Cypermethrin Substances 0.000 description 4
- KAATUXNTWXVJKI-UHFFFAOYSA-N cypermethrin Chemical compound CC1(C)C(C=C(Cl)Cl)C1C(=O)OC(C#N)C1=CC=CC(OC=2C=CC=CC=2)=C1 KAATUXNTWXVJKI-UHFFFAOYSA-N 0.000 description 4
- 229960005424 cypermethrin Drugs 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- WWUZIQQURGPMPG-UHFFFAOYSA-N (-)-D-erythro-Sphingosine Natural products CCCCCCCCCCCCCC=CC(O)C(N)CO WWUZIQQURGPMPG-UHFFFAOYSA-N 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 239000002068 microbial inoculum Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- WWUZIQQURGPMPG-KRWOKUGFSA-N sphingosine Chemical compound CCCCCCCCCCCCC\C=C\[C@@H](O)[C@@H](N)CO WWUZIQQURGPMPG-KRWOKUGFSA-N 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000001603 reducing effect Effects 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Soil Conditioners And Soil-Stabilizing Materials (AREA)
Abstract
The invention relates to a composition for accelerating pesticide residue in soil, which comprises the following components: cotton gauze, degrading bacteria and humus; the degradation fungus with the adhesion of humus after mixing is in on the cotton gauze, then will the cotton gauze is rolled up sealedly, and occupation space is less, the storage transportation of the degradation composition of being convenient for, after the sealing the cotton gauze is in the degradation fungus with become fibrous under the effect of humus, open the cotton gauze during use and seal, will the cotton gauze drops into the aquatic, and degradation fungus and humus of adhesion on the cotton gauze form degradation reagent through the dilution of water, can directly empty this degradation reagent in soil, and the cotton gauze that is fibrous can be degraded through the microorganism in the soil fast, can not cause air and soil pollution, uses in a flexible way.
Description
Technical Field
The invention relates to a composition for degrading pesticide residues, in particular to a composition for accelerating the degradation of pesticide residues in soil.
Background
Some chemical pesticides applied to soil have stable chemical properties and long persistence time, and a large amount of pesticides are continuously used to be continuously accumulated in the soil to influence the yield and quality of crops to a certain degree to form pollutants.
At present, the methods for solving the pesticide residue mainly include physical and chemical methods, which not only wastes manpower and physics and has poor residue reducing effect, but also easily causes secondary pollution of soil, air pollution is easily caused in some pesticide degrading processes, the transportation of a degrading reagent is troublesome in the degrading process, and cotton gauze adhered with humus and degrading bacteria has different states in different sealing periods, so that different degrading modes are realized.
Disclosure of Invention
The invention overcomes the defects of the prior art and provides the composition for accelerating the degradation of pesticide residues in soil.
In order to achieve the purpose, the invention adopts a technical scheme that: a composition for accelerating the degradation of pesticide residues in soil comprising: cotton gauze, degrading bacteria and humus; the cotton gauze is characterized in that the yarn count of warp and weft of the cotton gauze is between 32 and 64S/2; the degradation bacteria and the humus can be adhered to the cotton gauze, then the cotton gauze is rolled up and sealed, and when the degradation bacteria and the humus adhered to the cotton gauze are diluted or poured by water to form a degradation reagent.
In a preferred embodiment of the invention, the degrading bacteria are sphingomonas paucimobilis, the strain preservation number is ACCC01990, and the strain is purchased from Guangdong provincial microorganism culture collection center.
In a preferred embodiment of the invention, when the sealing time is 1-6 weeks, the cotton gauze can be directly paved on the soil surface, then water is sprayed on the surface layer of the cotton gauze, and the degradation bacteria and humus on the cotton gauze permeate into the soil to degrade the pesticide.
In a preferred embodiment of the invention, when the sealing time is more than 12 weeks, the cotton gauze becomes fibrous under the action of the degrading bacteria and the humus; putting the cotton gauze into water, stirring uniformly to obtain liquid, and then spraying the liquid through a spray head.
In a preferred embodiment of the invention, the humic substance is a macromolecular organic compound.
In a preferred embodiment of the invention, the humic substance can be one or more of humic acid, fulvic acid, humins.
In a preferred embodiment of the invention, the degradation agent can be poured directly into the soil; or
And filtering cotton gauze fibers in the degradation reagent and spraying the filtered cotton gauze fibers through a spray head.
In a preferred embodiment of the invention, cotton gauze applied with degrading bacteria and humus is sealed by a plastic film.
The invention solves the defects in the background technology, and has the following beneficial effects:
(1) when the sealing time of the cotton gauze is 1-6 weeks, the structure of the cotton gauze which forms the cotton gauze is not collapsed (the rotten amount of the cotton fiber which forms the cotton gauze is less than 20% of the total amount), at the moment, the cotton gauze can be paved on the surface of the soil, water is sprayed on the cotton gauze, humus and degradation bacteria on the cotton gauze permeate into the soil, so that the pesticide is degraded, and after the humus and the degradation bacteria on the cotton gauze are diluted, the cotton gauze on the soil can be decomposed through microorganisms in the soil, so that the pollution of the soil can not be caused. The degrading bacteria can increase the contact area of the degrading bacteria and the soil in a planar structure expansion mode of the cotton gauze, the contact surface of the degrading bacteria and the soil can be randomly adjusted in the expansion mode of the cotton gauze, and the use mode is flexible and changeable.
(2) When the sealing time is longer than 12 weeks, the structure of the cotton yarn changed by the cotton gauze is broken down (the rotting amount of the cotton fiber forming the cotton yarn is more than 30% of the total amount), the degradation bacteria and humus adhered to the cotton gauze are diluted by water and uniformly mixed to form a degradation reagent, the degradation reagent can be directly poured into soil, the cotton gauze can be rapidly degraded by microorganisms in the soil, secondary pollution of the air and the soil cannot be caused, and the use is flexible.
(3) The cotton gauze is used as a carrier of the mixture of the degrading bacteria and the humus, the mixture of the degrading bacteria and the humus can be conveniently stored, transported and used, in addition, the natural degradability of the cotton fiber can avoid secondary pollution, meanwhile, the efficacy and the performance of the degrading bacteria are not influenced, the storage and transportation mode of folding and rolling up the cotton gauze can greatly save space, and the excellent survival environment of the degrading bacteria can be provided.
(4) The degradation bacteria and the humus are adhered to the cotton gauze in an adhering mode and then sealed, so that the occupied space is reduced, the storage mode of the degradation composition is simpler and more flexible, and the transportation is convenient.
(5) During use, underground water can be used on the spot for diluting the degradation composition, fibers in the degradation reagent can be sprayed through the spray head after being diluted, during the period of sealing for 6-12 weeks, one mode of spreading on the soil surface for dripping and permeating or directly spraying after stirring is selected for pesticide degradation according to different environments, temperatures, external conditions and states of cotton gauze, and the use mode is more diversified and flexible.
Drawings
The invention is further illustrated with reference to the following figures and examples.
FIG. 1 is a graph of efficient cypermethrin in simulated sphingomonas paucimobilis agents degrading soil according to a preferred embodiment of the present invention;
Detailed Description
The invention will now be described in further detail with reference to the accompanying drawings and examples, which are simplified schematic drawings and illustrate only the basic structure of the invention in a schematic manner, and thus show only the constituents relevant to the invention.
A composition for accelerating the degradation of pesticide residue in soil is composed of cotton gauze, degrading bacteria and humus.
Specifically, the yarn count of warp and weft of the cotton gauze is between 32 and 64S/2; the degradation bacteria and the humus can be adhered to the cotton gauze, then the cotton gauze is rolled up and sealed, when the degradation bacteria and the humus which are adhered to the cotton gauze are diluted or poured by water to form a degradation reagent during use, the degradation bacteria and the humus are adhered to the cotton gauze in an adhesion mode, the cotton gauze applied with the degradation bacteria and the humus is sealed through a plastic package film, the occupied space is reduced, the storage mode of the degradation composition is simpler and more flexible, and the transportation is convenient.
Further, when the sealing time of the cotton gauze is 1-6 weeks, the cotton gauze can be directly paved on the soil surface, then water is sprayed on the surface layer of the cotton gauze, and the degradation bacteria and humus on the cotton gauze permeate into the soil to degrade pesticides.
Specifically, when the sealing time of the cotton gauze is 1-6 weeks, the structure of the cotton gauze which forms the cotton gauze is not collapsed (the rotting amount of the cotton fiber which forms the cotton gauze is less than 20% of the total amount), the cotton gauze is also a whole, the cotton gauze can be laid on the surface of the soil at the moment and sprinkled with water, humus and degrading bacteria on the cotton gauze permeate into the soil, so that the pesticide degradation is carried out, and after the humus and the degrading bacteria on the cotton gauze are diluted, the cotton gauze on the soil can be decomposed by microorganisms in the soil, so that the pollution to the soil can not be caused.
When the sealing time is longer than 12 weeks, the cotton gauze becomes fibrous under the action of degrading bacteria and humus; putting the cotton gauze into water, stirring uniformly to obtain liquid, and then spraying the liquid through a spray head.
Specifically, when the sealing time is longer than 12 weeks, the structure of the cotton yarn changed by the cotton gauze is broken down (the rotting amount of the cotton fiber forming the cotton yarn is more than 30% of the total amount), the degradation bacteria and humus adhered to the cotton gauze are diluted by water and uniformly stirred to form a degradation reagent, the degradation reagent can be directly poured into the soil, the cotton gauze can be rapidly degraded by microorganisms in the soil, secondary pollution to the air and the soil cannot be caused, and the use is flexible.
In a preferred embodiment of the invention, the degradation agent can be poured directly into the soil; or cotton gauze fibers in the degradation reagent are filtered and sprayed through a spray head, the degradation bacteria are sphingosine monad, humus is a macromolecular organic compound, the humus can be one or more of humic acid, fulvic acid and humins, a laboratory simulation experiment is carried out, as shown in figure 1, a change curve graph of the residual quantity of the sphingosine monad microbial inoculum in the degradation process of the high-efficiency cypermethrin in soil along with time is shown, the initial concentration of the high-efficiency cypermethrin in the soil is 50mg/kg, a certain proportion of bacterial liquid and humus are subjected to shake culture for 24 hours, supernatant is removed by centrifugation and then dried, the microbial inoculum is prepared and then sprayed into the soil, and the specific data of degradation are shown in table 1:
table 1 shows the degradation of highly effective cypermethrin in soil by sphingomonas paucimobilis preparations
Furthermore, underground water can be used for diluting the degradation composition in situ during use, fibers in the degradation reagent can be sprayed through a spray head after being diluted, during the period of sealing for 6-12 weeks, the pesticide degradation can be carried out by selecting one mode of dripping and permeating on the surface of soil or directly spraying after stirring according to different environments, temperatures, external conditions and states of cotton gauze, and the use mode is more diversified and flexible.
In light of the foregoing description of the preferred embodiment of the present invention, it is to be understood that various changes and modifications may be made by one skilled in the art without departing from the spirit and scope of the invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.
Claims (7)
1. A composition for accelerating the degradation of pesticide residues in soil comprising: cotton gauze, degrading bacteria and humus; the cotton gauze is characterized in that the yarn count of warp and weft of the cotton gauze is between 32 and 64S/2;
the degradation bacteria and the humus can be adhered to the cotton gauze, then the cotton gauze is rolled up and sealed, and when the degradation bacteria and the humus adhered to the cotton gauze are diluted or poured by water to form a degradation reagent;
when the sealing time is 1-6 weeks, the cotton gauze can be directly paved on the surface of the soil, then water is sprayed on the surface layer of the cotton gauze, and the degradation bacteria and humus on the cotton gauze permeate into the soil to degrade the pesticide.
2. The composition for accelerating the degradation of pesticide residues in soil according to claim 1, wherein the composition comprises: the degrading bacteria are sphingomonas paucimobilis, and the strain preservation number is ACCC 01990.
3. The composition for accelerating the degradation of pesticide residues in soil according to claim 1, wherein the composition comprises: when the sealing time is more than 12 weeks, the cotton gauze collapses the cotton yarn structure of the cotton gauze into loose fiber under the action of the degrading bacteria and the humus; putting the cotton gauze into water, stirring uniformly to obtain liquid, and then spraying the liquid through a spray head.
4. The composition for accelerating the degradation of pesticide residues in soil according to claim 1, wherein the composition comprises: the humus is a macromolecular organic compound.
5. The composition for accelerating the degradation of pesticide residues in soil according to claim 4, wherein the composition comprises: the humic substance can be one or more of humic acid, fulvic acid, and humins.
6. The composition for accelerating the degradation of pesticide residues in soil according to claim 1, wherein the composition comprises: the degradation agent can be poured directly into the soil; or filtering the cotton gauze fibers in the degradation reagent and spraying the cotton gauze fibers through a spray head.
7. The composition for accelerating the degradation of pesticide residues in soil according to claim 1, wherein the composition comprises: the cotton gauze applied with the degradation bacteria and the humus is sealed by a plastic packaging film.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811590653.4A CN109735343B (en) | 2018-12-25 | 2018-12-25 | Composition for accelerating degradation of pesticide residues in soil |
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| CN201811590653.4A CN109735343B (en) | 2018-12-25 | 2018-12-25 | Composition for accelerating degradation of pesticide residues in soil |
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| CN109735343B true CN109735343B (en) | 2021-03-19 |
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| CN107446862B (en) * | 2017-09-06 | 2023-12-15 | 北京双龙阿姆斯科技有限公司 | Microbial agent for degrading pesticide residues and preparation method thereof |
| CN107699498B (en) * | 2017-10-04 | 2020-09-25 | 北京康缘益生生物科技有限公司 | Composite microbial preparation for degrading organophosphorus pesticide and preparation method thereof |
| CN107475164B (en) * | 2017-10-09 | 2020-06-16 | 江苏绿华生物工程有限公司 | Complex microbial inoculant and application thereof in degrading pyrethroid pesticide residues in soil |
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Address after: 510665 Guangzhou, Guangdong, Tianhe District Province Road West, No. 318 Patentee after: Institute of chemical engineering, Guangdong Academy of Sciences Address before: No. 318, chebei West Road, Tianhe District, Guangzhou, Guangdong 510660 Patentee before: GUANGDONG RESEARCH INSTITITUTE OF PETROCHEMICAL AND FINE CHEMICAL ENGINEERING |