CN102764758A - DDT (dichloro-diphenyl-trichloroethane) contaminated soil combined remediation method by ultraviolet rays, alfalfa and DDT degrading bacteria - Google Patents
DDT (dichloro-diphenyl-trichloroethane) contaminated soil combined remediation method by ultraviolet rays, alfalfa and DDT degrading bacteria Download PDFInfo
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Abstract
The invention discloses a DDT (dichloro-diphenyl-trichloroethane) contaminated soil combined remediation method by ultraviolet rays, alfalfa and DDE degrading bacteria. The method is characterized by comprising steps of (1), irradiating DDT contaminated soil by the ultraviolet rays, and then ploughing and flattening the soil and filling water in the soil; (2), covering inoculants containing the DDT degrading bacteria and alfalfa rhizobia on alfalfa seeds; (3), coating the alfalfa seeds covered with the inoculants; (4), sowing the coated alfalfa seeds into the DDT contaminated soil; and (5), tending after seedlings emerge. DDT in the soil can be effectively decomposed, degraded and adsorbed, residual DDT in the soil is effectively reduced, and the DDT contaminated soil combined remediation method has the advantages of fast remediation speed, good effect, simplicity in operation, high processing quality, easiness in manual control and the like.
Description
Technical field
The present invention relates to the ecological, environmental protective field, specifically is that a kind of ultraviolet ray-alfalfa-DDT degradation bacteria of utilizing is united the method for repairing the DDT contaminated soil.
Background technology
The use of agricultural chemicals is to improve one of important measures of crop output per area.Use agricultural chemicals in the agricultural production in a large number and constantly, can cause its constantly accumulation in soil, cause the soil pollution by pesticides.Agricultural chemicals can influence output and the quality of crops, finally directly or indirectly influence population health via food chain through soil-crop system migration accumulation through approach polluted surface water and underground water such as soil eluviations.Residues of pesticides have caused very important harm to the aspects such as development of human health, soil, aquatic ecosystem and China's agricultural and rural economy.
Biconjugate chlorphenyl trichloroethanes (DDT) is a kind of typical organochlorine insecticide; Be to list one of 12 kinds of persistence organic pollutants in " about the Convention of Stockholm of persistence organic pollutant " in the first batch in, have the characteristic properties of persistence organic pollutant: persistence, half volatile, biological concentration property and high toxicity.Research shows that DDT has neurotoxicity, hepatotoxicity and Reproductive and developmental toxicity, can influence the endocrine function of organism, has typical three intoxicating property (carcinogenic, teratogenesis, mutagenesis).According to statistics, about more than 40 ten thousand tons of the accumulative total consumption of China DDT accounts for 20% of international consumption.Since nineteen eighty-three, production and the use of DDT are restricted, and only use as the intermediate and the vector control of dicofol at present.The use of DDT is controlled, and the DDT residual quantity is existing in China's soil obviously descends, but some regional residual quantities still are in higher level.The man small-sized dicofol of big dicofol manufacturing enterprises of existing 1 tame DDT manufacturing enterprise of China and 3 families and number enterprise, easy contaminated environment such as the waste liquid that in the production process of DDT and dicofol, produces, waste residue.The DDT manufacturing enterprise that has stopped production; Original production workshop and discarded production equipment possibly produce severe contamination to peripheral soil; If the soil etc. that receives the DDT severe contamination all might become the pollution sources of DDT without properly handling safely, environment is caused serious threat.DDT finally can accumulate in human body through surrounding medium or food chain, has a strong impact on the healthy of people.With regard to processing how to carry out the DDT discarded object, this problem of environment that reparation is polluted by DDT; A lot of experts have carried out big quantity research in succession; Developed the technology of many can control and degrade DDT discarded object, repairing environments; But wherein quite a few still is in laboratory stage, and the technology that is applied to engineering practice is few.And the relevant DDT research of China is few, mainly is the research of aspects such as relevant chemical oxidization method, bioanalysis.
Ultraviolet ray is the invisible ray that is positioned at daylight high energy district, and the difference according to ultraviolet ray self wavelength can be divided into three zones, i.e. short wave ultraviolet, ultraviolet B radiation and long wave ultraviolet with ultraviolet ray.The principle and the photochemical degradating of ultraviolet degradation remains of pesticide are approaching; It mainly is the effect that relies on medium wave ultraviolet light (wavelength 253.7nm); The chemical effect that the ultraviolet ray irradiation produces makes the two bond fissions of the main component of agricultural chemicals; Destroy the organic carbon of agricultural chemicals constituent and the combination between other element, the organic matter of difficult degradation is decomposed into low molecular compounds such as acids, alcohols, amine or corresponding oxide.Like the organophosphorus pesticide malathion, under the ultraviolet ray irradiation, generate malaoxon earlier, continue final phosphoric acid, sulfuric acid, carbon dioxide and the water of generating of reaction molecular fracture.
Phytoremediation is to utilize the absorption of root system and a kind of repair mode that the interior flourishing enzyme system of body is administered pollution.Plant, can also purify air with water body in rehabilitating soil, beautify the environment, prevents erosion as driving force with solar energy, and non-secondary pollution is specially adapted to the regional improvement of pollution in wide area.Because the difference of plant metabolism; So the mechanism of action of phytoremediation also is not quite similar; But can be divided into 5 kinds generally: (1) phytovolatilization: utilize plant that some volatile contaminants are absorbed in the plant, be translated into gaseous material then and discharge into the atmosphere; (2) plant extract: plant directly absorbs one or more pollutants and at the plant body accumulation, afterwards the plant results is handled; (3) plant degradation: the effect of the various enzymes that plant utilization itself produces, a kind of mode that the pollutant that absorbs in the plant is transformed and degrades; (4) plant rhizosphere effect: utilize the degradation of the various enzymes of plant rhizosphere microbe and root release to transform organic pollution; (5) plant stability: claim the plant passivation again,, pollutant is adsorbed in root system and soil surface, thereby reduce effective form of pollutant through the acting in conjunction of plant and soil.
Alfalfa (Medicago sativa L.) is a pulse family Papillionoideae clover, and is high because of its grass yield, be rich in characteristics such as protein, good palatability, adaptability are strong and be called as " king of herbage ".Alfalfa is a perennial plant, and general growth year can reach about 10 years, well developed root system, and cauline leaf is in great numbers.Summer, its dense branches and leaves can stop that rainwater washes away and surface runoff the physics of soil, and autumn and winter, face of land coverage was high, can not make table soil receive the erosion of strong wind, reduced dust storm content in the air.Loess Plateau Region in severe water and soil erosion is described as " water is protected the pioneer ".The prosperity of alfalfa root system is buried deeply, generally can reach 3-5 rice, and length can reach more than 10 meters; Therefore can absorb the moisture of deep subsoil, drought-resistant ability is just very high naturally, and on the huge root system a large amount of root nodules of life; Can carry out biological nitrogen fixation, 32.9 kilograms in every mu of about fixed nitrogen is equivalent to the ammonium sulfate of 2460kg; Improved soil fertility so greatly, this characteristic is highly beneficial to learotation system, can significantly improve the yield and quality of succession crop; And reduced the input of fertilizer, reduced the environmental pollution that causes because of a large amount of fertilizer inputs.In recent years; The method of carrying out the reparation of organic agricultural chemicals contaminated soil with alfalfa often has report; Use alfalfa to repair the method for pollution by polychlorinated biphenyles soil as disclosing in the patent documentation 1 (CN 101966529A), disclose in the patent documentation 2 (CN 102228899A) and used alfalfa to repair the method for amino benzenes compounds contaminated soil.
It is the technology of utilizing pollutant in the removal of microorganisms environment that microorganism is repaired.Microbe species is abundant, and metabolic pathway is various, is therefore having a high potential aspect conversion, degraded, all kinds of poisonous pollution compounds of decomposition.Microorganism can be divided into two big types to the mode of action of agricultural chemicals: one type is that microorganism directly acts on agricultural chemicals; Through the enzymatic reaction degrading pesticide; Several kinds of reaction types such as the normal microbe degradation of pesticides of saying belongs to this type of more, and the degraded mode mainly contains oxidation, dehydrogenation, reduction, hydrolysis, synthesize; Another kind of be activity through microorganism changed chemistry and physical environment and indirectly-acting in agricultural chemicals.
Yet, effective case of at present both at home and abroad the DDT contaminated soil being implemented ultraviolet ray-biological restoration also seldom, especially plant and microbe species select and the biological restoration Mechanism Study on also shortage go deep into systematic research.
Summary of the invention
The object of the present invention is to provide a kind of effective ways of the DDT of reparation contaminated soil.
Particularly, the present invention is that a kind of ultraviolet ray-alfalfa-DDT degradation bacteria of utilizing is united the method for repairing the DDT contaminated soil, it is characterized in that said method comprises the steps:
(1) the DDT contaminated soil is carried out ultraviolet ray irradiation, carry out the ploughing of soil, smooth and pour water then;
(2) on alfalfa seed, coat the microbial inoculum that contains DDT degradation bacteria and rhizobium melioti;
(3) alfalfa seed that is coated with microbial inoculum is carried out dressing;
(4) alfalfa seed with dressing is seeded in the DDT contaminated soil;
(5) carry out field management after emerging.
In the step (1), the preferred titanium dioxide (TiO that evenly sprays 20mg/L earlier on DDT contaminated soil surface
2) aaerosol solution, carry out the ultraviolet ray irradiation then.Also preferably after ultraviolet ray irradiation, spread fertilizer over the fields bentonite (100 kilograms/mu), and carry out the ploughing of soil, smooth and pour water with interior spreading fertilizer over the fields bentonite 24h at soil surface.In addition, the wavelength of ultraviolet ray irradiation is preferably 253.7nm, and irradiation time is preferably 1h-2h.
In the step (2); The seed of said alfalfa seed preferred maturity, full, free from insect pests; Preferred pseudomonad of said DDT degradation bacteria (Pseudomonas) and Sphingol single-cell (Sphingomonas paucimobilis), the preferred Sinorhizobium meliloti of said rhizobium melioti (Sinorhizobium meliloti).
The technological process of step (2) is preferably following: join the microbial inoculum that contains DDT degradation bacteria and rhizobium melioti in the sticker; After stirring, add alfalfa seed again, stir repeatedly; Make and all be stained with microbial inoculum on each seed, the mass ratio of seed and microbial inoculum is 1:7-10.Wherein, the sticker of use be preferably be selected from sodium cellulose glycolate, sodium carboxymethylcellulose, sodium alginate, starch, gelatin, the gum arabic more than a kind or 2 kinds.
The technological process of step (3) is preferably following: put into the dressing machine to the alfalfa seed that is obtained by step (2), shake mixing repeatedly through the dressing machine, make each seed all wrap one deck coating material, at last seed is put in the ventilation, it is for use to dry in the shade.Wherein, said coating material be preferably be selected from talcum powder, mica powder, the potter's clay powder more than a kind or 2 kinds.
In the step (4), it is following preferably to sow condition: seeding method is drilling, and application rate is 1.5-2 kilogram/mu, and depth of planting is 2-3 centimetre.
Method of the present invention can effectively be carried out decomposition, degraded and the absorption of DDT in the soil; DDT is residual in the minimizing soil; And have that reparation speed is fast, effective, simple to operate, treating capacity is big, be easy to characteristics such as artificial grasps, for large tracts of land reparation DDT contaminated soil provides effective ways.
Description of drawings
Fig. 1 is the flow chart of expression embodiment of the invention concrete steps.
The specific embodiment
The present invention is a kind of method of utilizing ultraviolet ray-bio combined reparation DDT contaminated soil.Particularly, the present invention is that a kind of ultraviolet ray-alfalfa-DDT degradation bacteria of utilizing is united the method for repairing the DDT contaminated soil, it is characterized in that said method comprises the steps:
(1) the DDT contaminated soil is carried out ultraviolet ray irradiation, carry out the ploughing of soil, smooth and pour water then;
(2) on alfalfa seed, coat the microbial inoculum that contains DDT degradation bacteria and rhizobium melioti;
(3) alfalfa seed that is coated with microbial inoculum is carried out dressing;
(4) alfalfa seed with dressing is seeded in the DDT contaminated soil;
(5) carry out field management after emerging.
The present invention repairs the DDT contaminated soil and is based on that following mechanism carries out: (1) ultraviolet irradiation can destroy the DDT molecular structure, especially ought use titanium dioxide (TiO simultaneously
2) time, TiO
2Under the ultraviolet ray irradiation, can carry out the multiphase photocatalysis oxidation, decomposing D DT, the repairing effect of raising DDT contaminated soil; (2) but utilize that alfalfa is perennial, well developed root system biological nitrogen fixation and can biological nature such as repair and combine the microorganism recovery technique to carry out the biological restoration of DDT contaminated soil to contaminated soil; Particularly; The microbial inoculum that contains the DDT degradation bacteria is through being coated on the alfalfa seed and passing through the further package action of coating material; Be combined on the alfalfa seed securely; After seed is broadcast in the soil, comprise in the microbial inoculum that the microorganism of DDT degradation bacteria will be along with the germination of seed, the growth and the extension of root system, distribute, breed along the root system of alfalfa; Thereby the double action of the phytoremediation of microbiological deterioration that can be through the DDT degradation bacteria and alfalfa is effectively decomposed, the DDT of degraded and absorption upper soll layer and even deep subsoil pollution.
In sum; The present invention repairs at ultraviolet chemistry, the microorganism of DDT degradation bacteria is repaired and the triple role of the phytoremediation of alfalfa under; Can effectively decompose, degrade and adsorb the DDT that upper soll layer and even deep subsoil pollute, thereby it is residual effectively to reduce in the soil DDT.Method of the present invention has that reparation speed is fast, effective, simple to operate, treating capacity is big, be easy to characteristics such as artificial grasps, is a kind of effective ways that are used for large tracts of land reparation DDT contaminated soil.
In above-mentioned steps (1), the preferred titanium dioxide (TiO that evenly sprays 20mg/L earlier on DDT contaminated soil surface
2) aaerosol solution, carry out the ultraviolet ray irradiation then.TiO
2Aaerosol solution is as catalyst, and its Catalysis Principles is TiO
2Through the ultraviolet ray irradiation, produce free electron and hole, and then produce the active oxygen of extremely strong oxidation, the oxidable decomposing D DT of active oxygen, thereby the repairing effect of raising DDT contaminated soil.
After the also preferred ultraviolet ray irradiation, spread fertilizer over the fields bentonite at soil surface.Bentonite (Bentonite) is to be main moisture clay pit with the montmorillonite, has characteristics such as swelling property, caking property, adsorptivity, catalytic, thixotropy, suspension and cation exchange.In soil, add bentonite as adsorbent, can effectively suppress the diafiltration of DDT in the soil that rainfall causes.Bentonitic consumption is generally 75-150 kilogram/mu, preferred 100 kilograms/mu.In addition, preferably bentonite spread fertilizer over the fields back 24 hours with interior to soil plough, smooth and pour water.Plough the degree of depth and be generally 15-30 centimetre, be preferably 20 centimetres.
In the step (1), preferably use the medium wave ultraviolet light of wavelength as 253.7nm, irradiation time is preferably 1-2h.
In above-mentioned steps (2), the seed of alfalfa seed preferred maturity, full, free from insect pests.Use such seed, can obtain better soil remediation effect.In addition, to the influence that microbial inoculum coats, preferably seed is cleaned in the clear water of normal temperature for fear of dust etc., dry under the natural conditions afterwards.
Rhizobium melioti is the one type of Gram-negative bacteria that supplies plant nutrient with clover symbiosis formation root nodule and with airborne nitrogen reduction ammonification.Through in microbial inoculum, adding rhizobium melioti, can promote the growth and the fixed nitrogen of alfalfa root system, thereby improve the repairing effect of soil.As rhizobium melioti, preferred Sinorhizobium meliloti (Sinorhizobium meliloti).
To the not special restriction of the DDT degradation bacteria of using; Can enumerate: bacteroides distasonis (Bacteroides distasonis), pseudomonad (Pseudomonas), Sphingol single-cell (Sphingomonas paucimobilis), golden spore exhibition tooth lead fungi (Phanerochaete Chrysosprium Burdsall) etc.; But from the angle that adapts to the agricultural chemicals ability and be prone to bring out sudden change, preferred pseudomonad.The dominant mechanism of pseudomonad degraded DDT is for to form new mutation through natural mutation, and produces induced enzyme by gene regulation, under new microbial enzyme effect, produces and the metabolic function of environment facies adaptation, thereby DDT is degraded.
In addition, can also in microbial inoculum, add Sphingol single-cell.Sphingol single-cell is at a bacterioid that has the huge applications potentiality aspect environmental protection and the commercial production, and aromatic is had metabolic capability very widely.
As microbial inoculum, for example can be respectively with Sinorhizobium meliloti in the YMA fluid nutrient medium, pseudomonad in the LB fluid nutrient medium, Sphingol single-cell cultivates in the LB fluid nutrient medium, mixes after collecting each bacterium, obtains composite bacteria agent capable.The mixing ratio of each bacterium (mass ratio) is preferably Sinorhizobium meliloti: pseudomonad: Sphingol single-cell=1:5 ~ 8:4 ~ 6, more preferably 1:8:6.
To the not special restriction of the concrete technology of step (2); But preferably use following method: join the microbial inoculum that contains DDT degradation bacteria and rhizobium melioti in the sticker, after stirring, add alfalfa seed again; Stir repeatedly, make and all be stained with microbial inoculum on each seed.Through using this method; Microbial inoculum can adhere on the seed through the effect of sticker securely; Microbial inoculum is difficult for coming off from seed in the sowing process; After planting the microorganism in the microbial inoculum can be effectively distributes, breeds along the root system of alfalfa, thereby has improved the biological restoration effect of soil.
In said method, the mass ratio of seed and microbial inoculum is preferably 1:7-10, more preferably 1:8.
To the not special restriction of the sticker that uses, but, preferably derive from the sticker of plant or animal or these stickers are carried out the sticker that modification obtains from preventing the angle of soil pollution.As such sticker, can enumerate sodium cellulose glycolate, sodium carboxymethylcellulose, sodium alginate, starch, gelatin, gum arabic etc.These stickers can use separately or two or more mixing is used.
To the not special restriction of the concrete technology of step (3); But preferably use following method: put into the dressing machine to the alfalfa seed that obtains by step (2); Shake mixing repeatedly through the dressing machine; Make each seed all wrap one deck coating material, at last seed is put in the ventilation, it is for use to dry in the shade.Through using this method, coating material is wrapped on the outer microbial inoculum that adheres to of seed equably, thereby has more effectively improved the compound action between microbial inoculum and the seed, improve the biological restoration effect of soil.
To the not special restriction of the coating material that uses, but from preventing the angle of soil pollution, preferred natural mineral material.As such coating material, can enumerate talcum powder, mica powder, potter's clay powder etc.These coating materials can use separately or two or more mixing is used.From extensive, the low-cost angle of material source, preferably talc powder.
The sowing condition of step (4) is preferably following: seeding method is drilling, and application rate is 1.5-2 kilogram/mu, and depth of planting is 2-3 centimetre.
Field management in the step (5) is meant timely and pours water, weed control and prevention and control of plant diseases, pest control etc.
Embodiment
Following embodiment can make those of ordinary skill in the art more fully understand the present invention, but does not limit the present invention in any way.
The standard sample of pesticide that experimental analysis is used: o, p '-DDT, p, p '-DDT standard sample, purity>99%, all available from U.S. Supelco company.
Experiment analytical method adopts " soil types and pollution concentration are repaired the DDT contaminated soil to laccase influence " (ecological environment, 2008 17 the 2nd phases of volume, 611-614 page or leaf).
Concrete steps are (as shown in Figure 1) as follows:
1. the DDT contaminated soil is made: experimental field be chosen in Dalian Nationality College's environment and resource institute experiment base; Experimental field area is 50 square metres; On July 10th, 2011 evenly sprayed with the solution that contains following DDT isomers (total concentration is 0.18mg/kg): o; P '-DDT (final concentration 0.08mg/kg), p, p '-DDT (final concentration 0.1mg/kg).Get 5 soil samples at random and carry out the DDT analysis.
2. the DDT contaminated soil is handled and farming: the titanium dioxide (TiO that earlier DDT contaminated soil surface is evenly sprayed 20mg/L
2) aaerosol solution (TiO
2Particle diameter 15nm, the big titanium white powder of the friend of Jinzhou City Manufacturing Co., Ltd produces), carry out the ultraviolet ray irradiation then, illumination wavelength is 253.7nm, irradiation time is 2h.After the ultraviolet ray irradiation, spread fertilizer over the fields 100 kilograms/mu of bentonites at soil surface, carry out afterwards 20 centimetres plough, smooth and pour water.
3. the screening of alfalfa seed, cleaning and drying: screen a public farming alfalfa (Medicago sativa L.cv.Gongnong NO.1) 0.15 kilogram in seed (available from Pasturage Science School of Jilin Agricultural Sciences Academy Grassland Research Institute) ripe, full, free from insect pests; After in the clear water of normal temperature, cleaning, dry under the natural conditions.
4. strain culturing and microbial inoculum are made: get Sinorhizobium meliloti (Sinorhizobium meliloti) (available from Shanghai Ai Yan bio tech ltd), pseudomonad (Pseudomonas) (grinding bio tech ltd available from Shanghai will) and Sphingol single-cell (Sphingomonas paucimobilis) (comforting living Industrial Co., Ltd. available from Shanghai); Respectively after shaking table is cultivated 48h in fluid nutrient medium under 28 ℃ the condition; Collect each bacterium, mix each bacterium and obtain composite bacteria agent capable.The culture medium of bacterial strain uses therefor and use is: Sinorhizobium meliloti-YMA fluid nutrient medium (80ml), pseudomonad-LB fluid nutrient medium (640ml), Sphingol single-cell-LB fluid nutrient medium (480ml); Sinorhizobium meliloti: pseudomonad: Sphingol single-cell (mass ratio)=1:8:6.
5. coat microbial inoculum: sticker uses sodium cellulose glycolate; Getting above-mentioned composite bacteria agent capable (about 1200 gram), to join mass concentration be in 5% the sodium cellulose glycolate aqueous solution (1000ml), after stirring, to add seed again; Stir repeatedly, make each seed all be stained with composite bacteria agent capable.
6. seed pelleting: coating material is a talcum powder; Put into the above-mentioned seed that is stained with composite bacteria agent capable and be equipped with in the talcous dressing machine, shake the mixing dressing repeatedly, make each seed all wrap one deck coating material through the dressing machine; At last seed is put in the ventilation, it is for use to dry in the shade.
7. sowing: carried out the drilling sowing on July 11st, 2011, application rate 1.5-2 kilogram/mu, depth of planting 2-3 centimetre.
8. field management: in time pour water after emerging, weed control and the prevention and control of plant diseases, pest control.
The check and analysis result is following:
Got 5 soil samples at random on November 10th, 2011 and carry out DDT and clear up analysis, o in the soil, p '-DDT, p, the content reduction amplitude of p '-DDT is respectively 38.52% and 34.28%, the overall average range of decrease 36.40%; Got 5 soil samples at random on July 10th, 2012 and carry out DDT and clear up analysis, o in the soil, p '-DDT, p, the content reduction amplitude of p '-DDT is respectively 45.39% and 40.07%, the overall average range of decrease 42.73%; Explain that method of the present invention can effectively repair the DDT contaminated soil.
Claims (12)
1. one kind is utilized ultraviolet ray-alfalfa-DDT degradation bacteria to unite the method for repairing the DDT contaminated soil, it is characterized in that said method comprises the steps:
(1) the DDT contaminated soil is carried out ultraviolet ray irradiation, carry out the ploughing of soil, smooth and pour water then;
(2) on alfalfa seed, coat the microbial inoculum that contains DDT degradation bacteria and rhizobium melioti;
(3) alfalfa seed that is coated with microbial inoculum is carried out dressing;
(4) alfalfa seed with dressing is seeded in the DDT contaminated soil;
(5) carry out field management after emerging.
2. method according to claim 1 is characterized in that, in the step (1), evenly sprays the titanium dioxide suspending solution of 20mg/L earlier on DDT contaminated soil surface, carries out the ultraviolet ray irradiation then.
3. method according to claim 1 and 2 is characterized in that, in the step (1), after the ultraviolet ray irradiation, spreads fertilizer over the fields bentonite at soil surface, and bentonite consumption is 100 kilograms/mu, spreads fertilizer over the fields bentonite 24h and carries out the ploughing of said soil, smooth and pour water with interior.
4. method according to claim 1 is characterized in that, in the step (1), the wavelength of ultraviolet ray irradiation is 253.7nm, and irradiation time is 1h-2h.
5. method according to claim 1 is characterized in that, in the step (2), said alfalfa seed is a seed ripe, full, free from insect pests.
6. method according to claim 1 is characterized in that, in the step (2), said DDT degradation bacteria is pseudomonad and Sphingol single-cell.
7. method according to claim 1 is characterized in that, in the step (2), said rhizobium melioti is a Sinorhizobium meliloti.
8. method according to claim 1; It is characterized in that the technological process of step (2) is following: join the microbial inoculum that contains DDT degradation bacteria and rhizobium melioti in the sticker, after stirring; Add alfalfa seed again; Stir repeatedly, make and all be stained with microbial inoculum on each seed, the mass ratio of seed and microbial inoculum is 1:7-10.
9. method according to claim 8 is characterized in that, said sticker be selected from sodium cellulose glycolate, sodium carboxymethylcellulose, sodium alginate, starch, gelatin, the gum arabic more than a kind or 2 kinds.
10. method according to claim 1; It is characterized in that; The technological process of step (3) is following: put into the dressing machine to the alfalfa seed that is obtained by step (2), shake mixing repeatedly through the dressing machine, make each seed all wrap one deck coating material; At last seed is put in the ventilation, it is for use to dry in the shade.
11. method according to claim 10 is characterized in that, said coating material be selected from talcum powder, mica powder, the potter's clay powder more than a kind or 2 kinds.
12. method according to claim 1 is characterized in that, in the step (4), and the seeding method drilling, application rate is 1.5-2 kilogram/mu, depth of planting is 2-3 centimetre.
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| CN106862262A (en) * | 2017-03-24 | 2017-06-20 | 四川大学 | A kind of soil biochemistry complex repairation agent and preparation method and application |
| CN107583945A (en) * | 2017-10-31 | 2018-01-16 | 爱土工程环境科技有限公司 | A kind of method of organic polluted soil production fired brick |
| CN110947754A (en) * | 2019-11-11 | 2020-04-03 | 沈阳大学 | Method for restoring farmland soil polluted by DDTs by using plant-reinforced microorganisms |
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| 张明星等: "DDT降解菌株DB-1的分离、系统发育及降解特性", 《中国环境科学》 * |
| 滕应等: "多氯联苯污染土壤菌根真菌-紫花苜蓿-根瘤菌联合修复效应", 《环境科学》 * |
| 潘淑颖: "土壤中有机氯农药DDT原位降解研究", 《中国博士学位论文全文数据库》 * |
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| CN106862262A (en) * | 2017-03-24 | 2017-06-20 | 四川大学 | A kind of soil biochemistry complex repairation agent and preparation method and application |
| CN106862262B (en) * | 2017-03-24 | 2020-08-04 | 四川大学 | Soil biochemical composite repairing agent and preparation method and application thereof |
| CN107583945A (en) * | 2017-10-31 | 2018-01-16 | 爱土工程环境科技有限公司 | A kind of method of organic polluted soil production fired brick |
| CN107583945B (en) * | 2017-10-31 | 2021-05-28 | 爱土工程环境科技有限公司 | Method for producing sintered bricks from organic contaminated soil |
| CN110947754A (en) * | 2019-11-11 | 2020-04-03 | 沈阳大学 | Method for restoring farmland soil polluted by DDTs by using plant-reinforced microorganisms |
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