CN109729871B - Method for promoting chloroplast stroma tubule generation in jute leaves - Google Patents
Method for promoting chloroplast stroma tubule generation in jute leaves Download PDFInfo
- Publication number
- CN109729871B CN109729871B CN201910171232.6A CN201910171232A CN109729871B CN 109729871 B CN109729871 B CN 109729871B CN 201910171232 A CN201910171232 A CN 201910171232A CN 109729871 B CN109729871 B CN 109729871B
- Authority
- CN
- China
- Prior art keywords
- jute
- polyethylene glycol
- solution
- leaves
- chloroplast
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses an application of polyethylene glycol in preparing a solution for enhancing the strong light resistance of jute, wherein the content of the polyethylene glycol in the solution is 20-40%. Also discloses a method for promoting the formation of chloroplast stroma tubules in jute leaves, which is to induce jute seedlings by using a solution containing polyethylene glycol. The method and the application of the invention can obviously promote the generation of chloroplast stroma tubules in jute leaves and further increase the strong light resistance of jute.
Description
Technical Field
The invention belongs to the field of biological cell biology, and particularly relates to a method for promoting the generation of chloroplast stroma tubules in jute leaves.
Background
Plastids (plastids) are bilayer membrane organelles capable of performing a fundamental function in plants. These functions range from the production and storage of starch in the white body (Leucoplast) to the conversion of light energy into sugars, the latter being an important function of chloroplasts. Matrix tubules (stromule) are referred to as tubular projections containing matrix, with a diameter of 0.35 to 1 μm and a length of 200 μm. While this finding has been for over 100 years, the matrix tubules have not received much attention before the matrix proteins are fused to GFP and thus observed. Plastids without chlorophyll and plastids found in regions of low plastid density exhibit a higher frequency of stromal tubule formation. Research suggests that chloroplast forms stroma tubules mainly have the following functions; the chloroplast can transmit substance and signal molecules, namely, the chloroplast can transport protein through a stroma tubule. It has also been found that small DNA molecules can be transported through the stroma tubules, and it has sometimes been observed that the chloroplast stroma tubules are in close contact with the plasma and nuclear membranes, and thus it is believed that the stroma tubules are likely to be used for signaling between the chloroplast and the cytoplasm and nucleus.
Jute (Corchorus capsularis) is also called Luoman and Luoman. Belongs to family of root tree (Tiliaceae) Corchorus genus (Corchorus) annual herbaceous bast fiber crop. Jute is a large variety, and two species having cultivation value are Corchorus capsularis L and Corchorus olitorius L, which are commonly called jute. The jute belongs to dicotyledonous short-day white-flower pollinated plants, the plant height is 3-5m, the stem is cylindrical, the stem thickness of the basal part is about 1.5cm, the stem color mainly comprises red and green, and the root system is straight; the true leaf is complete leaf, and the leaf is oval or acicular; the fruit is calabash fruit. Jute is one of the cheapest natural fibers, and the planting amount and the application are second to cotton.
However, jute is temperature-loving and strong light-resistant, the jute seedlings are difficult to culture under the general irregular temperature and illumination conditions, the jute often stops growing after the seedlings grow out by strong light irradiation for a long time in summer of high-temperature and strong illumination in China, and leaves are withered until the jute seedlings die in large.
However, despite years of research activity, the mechanism of matrix tubule formation is still unknown. Methods for inducing chloroplast stromal tubule formation in vitro are still inadequate.
Disclosure of Invention
In order to solve the technical problems, the inventor unexpectedly finds that the jute is treated by using the polyethylene glycol, so that the generation of matrix tubules in the jute leaves can be obviously induced, and the strong light resistance of the jute can be further enhanced. Thus, the present invention has been completed.
To this end, the present invention provides, in one aspect, the use of polyethylene glycol for preparing a solution for enhancing the strong light resistance of jute.
In one embodiment of the invention, the polyethylene glycol is present in the solution in an amount of 20 to 40%.
In the invention, the solution can be a liquid fertilizer, and can also be a solution formed by dissolving powder or solid polyethylene glycol in water, such as a liquid for spraying.
Further, the polyethylene glycol increases the strong light resistance of the jute plant by promoting the formation of chloroplast stroma tubules in the leaves of jute.
In an embodiment of the present invention, the polyethylene glycol is selected from at least one of polyethylene glycol 400, polyethylene glycol 2000, polyethylene glycol 6000, and polyethylene glycol 12000. In a preferred embodiment of the invention, the polyethylene glycol is polyethylene glycol 6000.
In an embodiment of the invention the polyethylene glycol is present in the solution in an amount of 20, 24, 29, 33 or 37%, in a preferred embodiment of the invention the polyethylene glycol is present in the solution in an amount of 29%.
The second aspect of the present invention provides a method for promoting the formation of chloroplast stroma tubules in jute leaves, comprising the step of inducing jute seedlings by using a solution containing polyethylene glycol.
In an embodiment of the present invention, the jute seedling is a seedling 5 to 7 weeks after the germination of jute seed.
In an embodiment of the present invention, the polyethylene glycol is selected from at least one of polyethylene glycol 400, polyethylene glycol 2000, polyethylene glycol 6000, and polyethylene glycol 12000. In a preferred embodiment of the invention, the polyethylene glycol is polyethylene glycol 6000.
In an embodiment of the invention the polyethylene glycol is present in the solution in an amount of 20, 24, 29, 33 or 37%, in a preferred embodiment of the invention the polyethylene glycol is present in the solution in an amount of 29%.
In an embodiment of the present invention, the roots of jute seedlings are placed in the solution for a certain period of time.
In a particular embodiment of the invention, said certain time is between 5 and 20 min.
In other embodiments of the present invention, the jute seedlings may also be subjected to root irrigation or foliar spray using a solution containing polyethylene glycol.
In other embodiments of the present invention, the jute seeds may also be soaked by spraying with a solution containing polyethylene glycol.
The invention has the advantages of
The method and the application of the invention can obviously promote the generation of chloroplast stroma tubules in jute leaves and further increase the strong light resistance of jute.
Drawings
Figure 1 shows a chloroplast with a stromal tubule.
Detailed Description
In order to make the technical problems, technical solutions and advantageous effects solved by the present invention more apparent, the present invention is further described in detail below with reference to the following embodiments.
Examples
The following examples are used herein to demonstrate preferred embodiments of the invention. It will be appreciated by those of skill in the art that the techniques disclosed in the examples which follow represent techniques discovered by the inventor to function in the invention, and thus can be considered to constitute preferred modes for its practice. Those of skill in the art should, in light of the present disclosure, appreciate that many changes can be made in the specific embodiments which are disclosed and still obtain a like or similar result without departing from the spirit or scope of the invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs and the disclosures and references cited herein and the materials to which they refer are incorporated by reference.
Those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following claims.
Example 1 chloroplast stroma tubule Induction
1. Biological conditions of plants
The jute was grown in a plant growth chamber under the conditions of 20 deg.C, 16 hr of light, 100 μmol photon intensity and 60% humidity.
2. Induction of matrix tubules
40 days after germination of jute, soaking the root of seedling in 20, 24, 29, 33, 37% polyethylene glycol (PEG6000) for 10min, washing with clear water for 3-5 times, and transplanting to growth chamber.
3. Chloroplast extraction and Observation
After the jute grows for 9-12 weeks, collecting the chloroplast of the jute leaves according to the following method:
1. selecting fresh jute leaves, cleaning, wiping to dry, removing leaf stalks and coarse veins, tearing into small fragments, weighing 3g, placing in a glass mortar, adding 10mL of 0.35mol/L NaCl solution, and homogenizing for 3-5 min.
2. The homogenate was filtered through 2 layers of nylon cloth in a 50mL beaker.
3. The filtrate was aliquoted into 2 centrifuge tubes, balanced with a balance and centrifuged at 1000r/min for 2 min. The precipitate was discarded.
4. The supernatant was centrifuged at 3000r/min for 5 min. And discarding the supernatant, wherein the precipitate is chloroplast.
5. The pellet was suspended in 0.35mol NaCl solution to make a temporary slide: a drop of chloroplast suspension was applied to a glass slide, covered with a glass slide, and observed under a microscope.
The results showed (fig. 1, table 1) that chloroplasts were green olives, and most of the chloroplasts in the induced jute leaves extended on their outer membrane to form a tubular bulge, i.e., a stroma tubule, which connects the chloroplasts into a net. In the uninduced jute leaves, only a small amount of stroma tubules or no stroma tubules exist, and chloroplasts are separately distributed.
TABLE 1
Example 2 study of jute resistance to Strong light Oxidation
The jute treated in example 1 was irradiated with light with an intensity of 700. mu. mol photons continuously for 14 days, and observed and recorded on day 15 for yellowing of the leaf of each treated jute.
Judging the strong light resistance of each treated jute according to the yellowing degree of the leaves, wherein the lighter the yellowing degree is, the stronger the strong light resistance is shown, and the weaker the strong light resistance is shown otherwise. Color was quantitatively described using a precision pH paper (5.4-7.0) as a reference, the leaf yellowing score was graded to 3, the green color (above pH 6.2) was maintained substantially at grade 1, the apparent yellowing (below pH 5.4) was graded to 3, and the interval between them (pH5.4-6.2) was graded to 2.
Observed on day 15 after the strong light irradiation, the yellowing degree of the jute leaves treated by the PEG6000 is different, wherein the yellowing degree of the jute leaves treated by the PEG6000 is lighter, the yellowing degree of the jute leaves treated by the 29 percent PEG6000 is lowest, and the difference of the yellowing degree of the jute leaves treated by the polyethylene glycol with different concentrations is not obvious (Table 2). The polyethylene glycol treatment can enhance the strong light resistance of the jute.
TABLE 2
All documents referred to herein are incorporated by reference into this application as if each were individually incorporated by reference. Furthermore, it should be understood that various changes and modifications of the present invention can be made by those skilled in the art after reading the above teachings of the present invention, and these equivalents also fall within the scope of the present invention as defined by the appended claims.
Claims (8)
1. The application of polyethylene glycol in preparing a solution for enhancing the strong light resistance of jute is characterized in that the content of the polyethylene glycol in the solution is 20-40%.
2. The use according to claim 1, wherein the polyethylene glycol is selected from at least one of polyethylene glycol 400, polyethylene glycol 2000, polyethylene glycol 6000 and polyethylene glycol 12000.
3. Use according to claim 1, characterized in that the polyethylene glycol is present in the solution in a content of 29%.
4. A method for promoting the formation of chloroplast stroma tubules in jute leaves, which is characterized in that: comprises the step of inducing jute seedlings by using a solution containing polyethylene glycol.
5. The method as claimed in claim 4, wherein the jute seedlings are seedlings 5-7 weeks after germination of jute seeds.
6. The method according to claim 4, wherein the polyethylene glycol is at least one selected from the group consisting of polyethylene glycol 400, polyethylene glycol 2000, polyethylene glycol 6000, and polyethylene glycol 12000.
7. The method according to any one of claims 4 to 6, wherein the polyethylene glycol is present in the solution in an amount of 29%.
8. The method according to any one of claims 4 to 6, wherein the roots of the young jute seedlings are placed in the solution for 5 to 20 min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910171232.6A CN109729871B (en) | 2019-03-07 | 2019-03-07 | Method for promoting chloroplast stroma tubule generation in jute leaves |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910171232.6A CN109729871B (en) | 2019-03-07 | 2019-03-07 | Method for promoting chloroplast stroma tubule generation in jute leaves |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109729871A CN109729871A (en) | 2019-05-10 |
| CN109729871B true CN109729871B (en) | 2020-11-24 |
Family
ID=66369766
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910171232.6A Expired - Fee Related CN109729871B (en) | 2019-03-07 | 2019-03-07 | Method for promoting chloroplast stroma tubule generation in jute leaves |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109729871B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107173026A (en) * | 2017-06-30 | 2017-09-19 | 合肥卓畅农业科技有限公司 | A kind of implantation methods for improving high quality soybean yield |
| KR101884411B1 (en) * | 2017-07-13 | 2018-08-02 | 주식회사 뷰티채널 | Functional cosmetic composition for brighting and moisturizing cream |
-
2019
- 2019-03-07 CN CN201910171232.6A patent/CN109729871B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107173026A (en) * | 2017-06-30 | 2017-09-19 | 合肥卓畅农业科技有限公司 | A kind of implantation methods for improving high quality soybean yield |
| KR101884411B1 (en) * | 2017-07-13 | 2018-08-02 | 주식회사 뷰티채널 | Functional cosmetic composition for brighting and moisturizing cream |
Non-Patent Citations (3)
| Title |
|---|
| GFP movement between chloroplasts;John C. Gray;《Nature Biotechnology》;20000131;第1146页 * |
| PEG模拟干旱胁迫对11份黄麻种子萌发的效应;杨柳 等;《南方农业学报》;20111231;第42卷(第7期);第715-718页 * |
| 黄麻β-酮脂酰-CoA合酶(KCS)基因分离与功能鉴定;张高阳 等;《分子植物育种》;20181231;第16卷(第20期);第6718-6722页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109729871A (en) | 2019-05-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104429472B (en) | Dendrobiumpaxt natural forest implantation high-yield culture method | |
| CN101822220B (en) | Method for culturing and rapidly propagating stem tip tissue of rare cymbidium goeringii | |
| CN101103701B (en) | Curcuma wenyujin detoxicating and quick reproduction method | |
| Logan et al. | Rapid in vitro propagation of virus-indexed gladioli | |
| CN104813917B (en) | A kind of breeding method of Fructus Capsici | |
| CN101536669B (en) | High-efficiency polymerization method for apple cross breeding | |
| CN102835316A (en) | Method for removing grape virus disease and rapidly propagating seedling for cabernet gernischet grape variety | |
| Shiji et al. | Micropropagation for rapid multiplication of planting material in cassava (Manihot esculenta Crantz) | |
| CN115720851A (en) | Sugarcane somatic embryo and induction method thereof | |
| CN106896008A (en) | A kind of preparation method of spot thatch plant root tip meristematic zone chromosome specimen | |
| CN109468333A (en) | Citrus laccase family gene CsiLAC4 and its application | |
| CN102612962A (en) | Method for identifying resistance of sweet potatoes in seedling stage to black spot | |
| CN105706923B (en) | A kind of method for screening the drought-enduring variant of sugarcane | |
| CN109729871B (en) | Method for promoting chloroplast stroma tubule generation in jute leaves | |
| CN108967196A (en) | A kind of cultural method of cole in vitro sporule regeneration plant | |
| CN105638435B (en) | Utilize the method that honeybee is Crops ' pollination | |
| CN105340746A (en) | Method for cultivating octoploid lowland type switchgrass | |
| KR100533793B1 (en) | Mass propagation Method for Stock and Adventitious Root of Acanthopanax Koreanum Nakai Using Bioreactor | |
| CN112273077A (en) | Living body preservation method for solanum vegetable germplasm resources | |
| KR100302206B1 (en) | In-flight mass production and forge transplanting method | |
| Jaakola et al. | Micropropagation of bilberry and lingonberry | |
| KR100322351B1 (en) | A method for producing seedlings of Acanthopanax koreanum NAKAI by tissue culture | |
| CN115997683B (en) | Efficient creating method for tetraploid betula platyphylla | |
| CN110637621B (en) | Eight-step tea cutting seedling cultivation method | |
| KR100569507B1 (en) | The culturing apparatus for periodically submerged culture and the culturing method thereof in the somatic embryos culture for mass production of Eleutherococcus senticosus seedlings |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20201124 |