CN109729871A - A method of promote jute blade Chloroplast matrix tubule to generate - Google Patents
A method of promote jute blade Chloroplast matrix tubule to generate Download PDFInfo
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- CN109729871A CN109729871A CN201910171232.6A CN201910171232A CN109729871A CN 109729871 A CN109729871 A CN 109729871A CN 201910171232 A CN201910171232 A CN 201910171232A CN 109729871 A CN109729871 A CN 109729871A
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- jute
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Abstract
It is 20-40% that the invention discloses polyethylene glycol preparing the purposes in the solution for enhancing the resistance to strong light ability of jute, content of the polyethylene glycol in the solution.A kind of method that promotion jute blade Chloroplast matrix tubule generates is also disclosed, i.e., jute seedling is induced using the solution containing polyethylene glycol.The method and use of the present invention can remarkably promote the generation of jute blade Chloroplast matrix tubule, and further increase the resistance to strong light ability of jute.
Description
Technical field
Biological cell field of biology of the invention, and in particular to a kind of promotion jute blade Chloroplast matrix tubule is raw
At method.
Background technique
Plastid (Plastid) is double layer membrane organelles, and basic function can be played in plant.The range of these functions from
It is the critical function of chloroplaset that the starch in leucoplast (Leucoplast), which is manufactured and stored, to sugar, the latter is converted light energy into.
Matrix tubule (stromule) is referred to the tubular protrusion containing matrix, and a diameter of 0.35 μm to 1 μm, length is~200 μm.
Matrix tubule rises in the tubular protrusion on plastid, although this discovery is pass by more than 100 years, by stromatin with
Before GFP fusion is to be observed, there is no paid close attention to matrix tubule too much.There is no the plastid of chlorophyll and close in plastid
It spends the plastid found in low region and shows that higher matrix tubule forms frequency.Research thinks that chloroplaset forms matrix tubule
It is mainly the following effect；The transmitting of substance and signaling molecule can be carried out between chloroplaset, that is to say, that chloroplaset it
Between can pass through matrix tubule carry out albumen transhipment.Additionally, it is observed that some small DNA moleculars also can by matrix tubule into
Row transport, sometimes also it is observed that chloroplast stroma tubule can be in close contact plasma membrane and nuclear membrane, therefore it is believed that matrix
Tubule is more likely used for carrying out signal transduction between chloroplaset and cytoplasm and nucleus.
Jute (Corchorus capsularis) also known as network fiber crops, green fiber crops.Shu Genshu section (Tiliaceae) Corchorus
(Corchorus) annual herb bast fiber crop.There are many kind of Corchorus, and two kinds with cultivating value are circle fruit kinds
Jute is referred to as in jute (Corchorus capsularis L.) and corchorus olitorius (C.olitoriustL.), cultivation.Corchorus
Dicotyledonous short-day white flower pollinated plant, 3-5 meters of plant height, stem is cylindrical, and base portion stem thickness 1.5cm or so, stem color mainly has red, green
Two kinds, the system of taproot；True leaf is complete leaf, blade oval or lanceolar；Fruit is calabash fruit.Jute is most cheap natural fiber
One of, plantation amount and the extensive of purposes are all only second to cotton.
But jute happiness temperature, resistance to strong light, general irregular temperature and illumination condition are difficult to cultivate jute seedling, and jute is long
The summer that season shines on the occasion of China's High temperature, the strong illumination of long period often stop growing after its seedling grows, leaf
Here piece withers, until large stretch of dead.
However, the mechanism that matrix tubule is formed is still unknown although having carried out years of researches activity.It lures in vitro
The method for leading the formation of chloroplast stroma tubule still is apparent not enough.
Summary of the invention
In order to solve the above-mentioned technical problem in, inventor it was unexpectedly observed that using polyethylene glycol handle jute, can be significant
The generation of jute blade mesostroma tubule is induced, and further enhances the resistance to strong light ability of jute.Thereby completing the present invention.
For this purpose, one aspect of the present invention, which provides polyethylene glycol, is preparing the use in the solution for enhancing the resistance to strong light ability of jute
On the way.
In one embodiment of the invention, content of the polyethylene glycol in the solution is 20-40%.
In the present invention, the solution can be liquid fertilizer, be also possible to powder or solid polyethylene glycol formation soluble in water
Solution, such as use liquor by spraying.
Further, the polyethylene glycol increases Corchorus by promoting jute blade Chloroplast matrix tubule to generate
The resistance to strong light ability of plant.
In embodiments of the invention, the polyethylene glycol is selected from polyethylene glycol 400, polyethylene glycol 2000, poly- second two
At least one of alcohol 6000 and polyethylene glycol 12000.In a preferred embodiment of the invention, the polyethylene glycol is poly- second
Glycol 6000.
In embodiments of the invention, content of the polyethylene glycol in the solution be 20,24,29,33 or
37%, in a preferred embodiment of the invention, content 29% of the polyethylene glycol in the solution.
The second aspect of the present invention provides a kind of method that promotion jute blade Chloroplast matrix tubule generates, including benefit
The step of jute seedling is induced with the solution containing polyethylene glycol.
In embodiments of the invention, the jute seedling is 5-7 weeks seedling after jute germination.
In embodiments of the invention, the polyethylene glycol is selected from polyethylene glycol 400, polyethylene glycol 2000, poly- second two
At least one of alcohol 6000 and polyethylene glycol 12000.In a preferred embodiment of the invention, the polyethylene glycol is poly- second
Glycol 6000.
In embodiments of the invention, content of the polyethylene glycol in the solution be 20,24,29,33 or
37%, in a preferred embodiment of the invention, content 29% of the polyethylene glycol in the solution.
In embodiments of the invention, the root of jute seedling is placed in certain time in the solution.
In specific embodiments of the present invention, the certain time is 5-20min.
In other embodiments of the invention, it also can use the solution containing polyethylene glycol and jute seedling carried out
Pouring root or foliar spray.
In other embodiments of the invention, also can use the solution containing polyethylene glycol spray jute seed into
Row impregnates.
Beneficial effects of the present invention
The method and use of the present invention can remarkably promote the generation of jute blade Chloroplast matrix tubule, and further
Increase the resistance to strong light ability of jute.
Detailed description of the invention
Fig. 1 shows the chloroplaset with matrix tubule.
Specific embodiment
In order to which the technical problems, technical solutions and beneficial effects solved by the present invention is more clearly understood, below in conjunction with
Embodiment, the present invention will be described in further detail.
Embodiment
Following example is used herein to demonstration the preferred embodiments of the invention.Those skilled in the art, it will be appreciated that under
State the technology disclosed in example represent inventor discovery can be used for implementing technology of the invention, therefore can be considered as implementation this
The preferred embodiment of invention.But those skilled in the art should be understood that specific reality disclosed herein according to this specification
Many modifications can be made by applying example, still can be obtained identical or similar as a result, rather than away from the spirit or scope of the present invention.
Unless otherwise defined, the term of all technologies as used herein and science, and the technology in fields of the present invention
Personnel institute is normally understood equivalent in meaning, and being disclosed reference and their materials of reference will all be incorporated.
Those skilled in the art will recognize or just will appreciate that by routine test many described here
Invention specific embodiment many equivalent technologies.These will equally be comprised in claims.
The induction of 1 chloroplast stroma tubule of embodiment
1. plant biological condition
Jute grows in phytotron, and condition is set as 20 DEG C, and light application time is 16 hours, and luminous intensity is 100 μm of ol
Photon, humidity 60%.
2. matrix tubule inductive formation
40 days after jute germination, seedling root is separately immersed in 20,24,29,33,37% polyethylene glycol (PEG6000)
Middle 10min, is cleaned 3-5 times with clear water, is transplanted into growth room.
3. chloroplaset extracts and observation
After jute grows 9-12 weeks, jute blade chloroplaset is collected by following method:
1. choosing fresh jute blade, clean and dry rear disleaf stalk and thick arteries and veins, be torn into fine grained chippings, claims 3g to be put in glass and grind
In alms bowl, the NaCl solution of 10mL 0.35mol/L is added, is homogenized 3-5min.
2. homogenate is with 2 layers of nylon cloth filtering in 50mL beaker.
3. filtrate is divided equally in 2 centrifuge tubes, balance trim, 2min is centrifuged under 1000r/min revolving speed.Discard precipitating.
4. supernatant is centrifuged 5min under 3000r/min revolving speed.It discards supernatant, precipitating is chloroplaset.
5. the precipitating NaCl solution of 0.35mol is suspended, it is made into interim slide: taking drop chloroplaset suspension drop in load glass
On piece covers slide, observes under the microscope.
(Fig. 1, table 1) as the result is shown, chloroplaset are green olive-type, also, in the jute blade after inducing, big leafy
Green body extends to form tubulose protrusion, i.e. matrix tubule on its outer membrane, chloroplaset is linked to be netted.And the roundpod jute leaf without induction
In piece, only a small amount of matrix tubule or there is no matrix tubule, chloroplaset is in independent dispersed distribution.
Table 1
The resistance to strong photooxidation research of 2 jute of embodiment
The jute that will respectively be handled in embodiment 1, increase intensity of illumination to 700 μm of ol photons, continuous strong illumination 14 days, the
The yellowing of each processing roundpod jute leaf piece is recorded in observation in 15 days.
Each resistance to strong light ability of processing jute is judged with yellowing leaf degree, yellowing gentlier shows resistance to strong light ability more
By force, on the contrary then weaker.Using special pH test paper (5.4-7.0) as referring to color quantification description is carried out, yellowing leaf degree divides 3
Grade, basic holding green (PH6.2 or more) are 1 grade, hence it is evident that yellow (PH5.4 or less) is 3 grades, and fall between (PH5.4-
It 6.2) is 2 grades.
It observes within the 15th day after strong illumination, it is each to handle roundpod jute leaf piece yellowing difference, wherein to be handled through PEG6000
Jute blade yellowing is lighter, wherein 29%PEG6000 processing yellowing is minimum, at other various concentration polyethylene glycol
Yellowing difference is unobvious (table 2) between reason.Show that the resistance to strong light ability of jute can be enhanced in polyethylene glycol processing.
Table 2
All references mentioned in the present invention is incorporated herein by reference, independent just as each document
It is incorporated as with reference to such.In addition, it should also be understood that, after reading the above teachings of the present invention, those skilled in the art can
To make various changes or modifications to the present invention, such equivalent forms equally fall within model defined by the application the appended claims
It encloses.
Claims (10)
1. polyethylene glycol is preparing the purposes in the solution for enhancing the resistance to strong light ability of jute, which is characterized in that the poly- second
Content of the glycol in the solution is 20-40%.
2. purposes according to claim 1, which is characterized in that the polyethylene glycol is by promoting jute blade Chloroplast
Matrix tubule generates to increase the resistance to strong light ability of jute platymiscium.
3. purposes according to claim 1 to 2, which is characterized in that the polyethylene glycol is selected from polyethylene glycol 400, gathers
At least one of ethylene glycol 2000, Macrogol 6000 and polyethylene glycol 12000.
4. purposes according to claim 1 to 2, which is characterized in that content of the polyethylene glycol in the solution
It is 29%.
5. it is a kind of promote jute blade Chloroplast matrix tubule generate method, it is characterised in that: including the use of contain poly- second
The step of solution of glycol induces jute seedling.
6. according to the method described in claim 5, it is characterized in that, the jute seedling is 5-7 weeks children after jute germination
Seedling.
7. according to the method described in claim 5, it is characterized in that, the polyethylene glycol is selected from polyethylene glycol 400, polyethylene glycol
2000, at least one of Macrogol 6000 and polyethylene glycol 12000.
8. according to any method of claim 5-7, which is characterized in that content of the polyethylene glycol in the solution
It is 29%.
9. according to any method of claim 5-7, which is characterized in that the root of jute seedling to be placed in the solution
Certain time.
10. according to the method described in claim 9, it is characterized in that, the certain time is 5-20min.
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| CN201910171232.6A CN109729871B (en) | 2019-03-07 | 2019-03-07 | Method for promoting chloroplast stroma tubule generation in jute leaves |
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| CN201910171232.6A CN109729871B (en) | 2019-03-07 | 2019-03-07 | Method for promoting chloroplast stroma tubule generation in jute leaves |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107173026A (en) * | 2017-06-30 | 2017-09-19 | 合肥卓畅农业科技有限公司 | A kind of implantation methods for improving high quality soybean yield |
| KR101884411B1 (en) * | 2017-07-13 | 2018-08-02 | 주식회사 뷰티채널 | Functional cosmetic composition for brighting and moisturizing cream |
-
2019
- 2019-03-07 CN CN201910171232.6A patent/CN109729871B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107173026A (en) * | 2017-06-30 | 2017-09-19 | 合肥卓畅农业科技有限公司 | A kind of implantation methods for improving high quality soybean yield |
| KR101884411B1 (en) * | 2017-07-13 | 2018-08-02 | 주식회사 뷰티채널 | Functional cosmetic composition for brighting and moisturizing cream |
Non-Patent Citations (4)
| Title |
|---|
| JOHN C. GRAY: "GFP movement between chloroplasts", 《NATURE BIOTECHNOLOGY》 * |
| 张高阳 等: "黄麻β-酮脂酰-CoA合酶(KCS)基因分离与功能鉴定", 《分子植物育种》 * |
| 杨柳 等: "PEG模拟干旱胁迫对11份黄麻种子萌发的效应", 《南方农业学报》 * |
| 翟中和 等: "《细胞生物学（都4版）》", 30 June 2011, 高等教育出版社 * |
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