CN109718281B - Traditional Chinese medicine compound medicine for treating rheumatoid arthritis and preparation method - Google Patents
Traditional Chinese medicine compound medicine for treating rheumatoid arthritis and preparation method Download PDFInfo
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Abstract
The invention belongs to the field of medicines, and particularly relates to a traditional Chinese medicine compound medicine for treating rheumatoid arthritis and a preparation method thereof. The traditional Chinese medicine compound medicine for treating rheumatoid arthritis is at least prepared from honeysuckle and clematis root. Further, the traditional Chinese medicine compound medicine for treating rheumatoid arthritis is prepared from honeysuckle, rhodiola root, clematis root and sphagnum planiculum, wherein the medicinal materials are extracted by an alcohol extraction and water precipitation method. Wherein the weight parts of the honeysuckle, the rhodiola rosea, the clematis root and the sphagnum planiculosum are 90 parts by weight of the honeysuckle, 90 parts by weight of the rhodiola rosea, 30 parts by weight of the clematis root and 3-7 parts by weight of the sphagnum planiculosum. The traditional Chinese medicine compound medicine has a good effect on rheumatoid arthritis, and is simple in formula and high in safety.
Description
Technical Field
The invention belongs to the field of medicines, and particularly relates to a traditional Chinese medicine compound medicine for treating rheumatoid arthritis and a preparation method thereof.
Background
Rheumatoid arthritis is an autoimmune disease commonly seen in the middle-aged and elderly. The disease continuously and slowly affects a plurality of joints, so that the joints are deformed, and the disability of a patient is easily caused, and the disease is one of the main chronic diseases which cause the incapacity of the patient. It is mainly manifested by joint swelling and pain, morning stiffness, etc. The exact etiology and pathogenesis of the disease are not clearly researched from the perspective of western medicine or traditional Chinese medicine; no matter whether western medicines or traditional Chinese medicines are adopted, the diseases cannot be completely cured at present, and the treatment purpose is still to control the progress of the disease. From the influence population of the disease, the disease incidence rate is about 1%, the Chinese incidence rate is about 0.4%, and the influence population is still huge in consideration of the population base problem. The research on the rheumatoid arthritis medicine is also the requirement which needs to be met by the clinical application at present.
The current treatment of rheumatoid arthritis by western medicine is mainly based on the control of inflammation-related factors, such as the use of non-steroidal anti-inflammatory drugs like aspirin, glucocorticoids, disease-relieving antirheumatic drugs, etc. Where nonsteroidal anti-inflammatory drugs tend to have difficulty controlling disease progression; glucocorticoid drugs have obvious side effects and are not suitable for long-term administration. The new biological medicine is mainly antibody medicine for interleukin and tumor necrosis factor as cell factor indexes. However, since many cytokines are involved in rheumatoid arthritis, and monoclonal antibodies often act only on a single cytokine, their effects are limited unless targeted therapy is possible for cytokines that play critical roles upstream. Due to the complex mechanism, it is still impossible to determine which cytokines are key cytokines.
The application history of the traditional Chinese medicine in the field of rheumatoid arthritis is long. Complete cure of rheumatoid arthritis is also difficult. In the traditional Chinese medicine theory, rheumatoid arthritis is caused by invasion of wind-cold-dampness evil and mixed causes of cold-dampness, scouring wind, turbid phlegm and blood stasis. The pathogenic factors are long in accumulation and difficult to remove, pathogenic conditions are prolonged, and recovery is difficult, so that the wind-dispelling and dampness-removing principle is the main medication principle, and the dampness is not separated from the temperature during treatment, the dampness can be dissolved by the temperature, the cold-dampness evil is favorably removed, and the warm-heat medicines are matched with the wind-dispelling and dampness-removing medicines. However, it is also considered that most of rheumatoid arthritis is caused by heat toxin, most of cold and heat are complicated, yin deficiency is the intrinsic cause of the disease, invasion of various pathogens is the external cause, and blood stasis is caused by deficiency-heat accumulation and meridian obstruction, so that cold, cool and blood circulation-promoting medicines can also be formulated to treat rheumatoid arthritis. The conflict of theoretical analysis is related to the complex etiology and complex manifestations of rheumatoid arthritis. For the traditional Chinese medicine rheumatoid arthritis model, the current model is difficult to truly simulate the process of wind, cold and dampness to participate in the disease, the basic modeling mechanism is still based on the basic principle of inflammation, the factors of wind, cold, dampness and the like are only assisted, and the simulation effect is difficult to estimate. In terms of western medicine theory and western medicine research and development process, research and development of medicines such as rheumatoid arthritis are also animal models based on inflammation in the first place.
The analysis of the traditional Chinese medicine for treating rheumatoid arthritis at present from single traditional Chinese medicine and compound medicine accords with the anti-inflammatory theory of western medicine. Such as honeysuckle, large-leaved gentian, clematis root, Chinese taxillus twig, fourstamen stephania root, Chinese ivy vine, suberect spatholobus stem, dandelion, glabrous greenbrier rhizome, rhizoma bolbostemmae, weeping forsythia capsule, spreading hedyotis herb, amur corktree bark, bunge corydalis herb, lightyellow sophora root, uniflower swisscentaury root, cape jasmine fruit, honeysuckle stem, white paeony root, red paeony root, szechuan lovage rhizome, suberect spatholobus stem, Chinese angelica, pseudo-ginseng, zedoary, rhizoma wenyujin concisenia, frankincense, myrrh, sappan wood, garden balsam stem, rhubarb, figwort root, paniculate swallowwort root, common clubmoss herb, Chinese starjasmine stem, pyrola herb, great burdock fruit, yam rhizome, liquorice and the like, and the anti-inflammatory active parts and the active ingredients of the ingredients, such as astilb. Animal Chinese medicine commonly used for rheumatoid arthritis also has antiinflammatory effect, such as Scolopendra, Scorpio, etc. Therefore, the single medicine or the compound medicine for treating the rheumatoid arthritis by the traditional Chinese medicine has a material basis which accords with the western medicine theory, which is undoubtedly. This is why TCM is still effective no matter whether the theory of TCM is controversial or conflicting. However, even if all the compound medicines have anti-inflammatory components, the change of the proportion of the anti-inflammatory components can also cause the obvious difference of the curative effect, and the reason that the components of the traditional Chinese medicine compound are still not thoroughly researched and clearly understood so far is the reason that the traditional Chinese medicine compound is far more complicated than the western medicine compound, and is also the unique point of the traditional Chinese medicine compound. The processing and extraction processes also introduce some newly generated components or remove some original components through chemical reactions, so the exact mechanism of the drug effect change caused by the compound formula ratio is difficult to study and clarify.
At present, the compatibility research of the traditional Chinese medicine is mainly carried out on a medicine pair or a typical small compound, and only part of the composing mechanism and the extraction method are clarified. However, the reason why the attenuation and synergy of most of the Chinese herbal compound occur is not clear at present. The study of complex formulations is more difficult to elucidate as it involves an infinite combination of formulation ingredient changes and formulation dosage changes. Based on the problem, the increase of the traditional Chinese medicine flavor of the compound traditional Chinese medicine is relatively easy, and the simplification or the optimized replacement of the traditional Chinese medicine flavor is difficult.
Disclosure of Invention
In view of the prior art, the invention provides a traditional Chinese medicine compound medicine for treating rheumatoid arthritis on one hand.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a Chinese medicinal compound medicine for treating rheumatoid arthritis is prepared from flos Lonicerae and radix Clematidis.
For the sake of clarity, it is noted that: the flos Lonicerae is Lonicera japonica Thunb of Caprifoliaceae,
Dried flower buds of Lonicera hypoglauca Miq, Lonicera confusa DC, or Lonicera dasystyla Rehd. The radix Clematidis is dried root and rhizome of Clematis chinensis Osbeck, Clematis hexapetala pall or Clematis manshurica rupr of Ranunculaceae.
The combination of the two traditional Chinese medicines has the treatment effect on rheumatoid arthritis, but the effect is not ideal. Therefore, the formula is improved.
In an improved formula, preferably, the traditional Chinese medicine compound medicine for treating rheumatoid arthritis is prepared from the following traditional Chinese medicines
The composition is as follows: honeysuckle, rhodiola root, clematis root and sphagnum planiculosum; the Chinese medicinal materials are extracted by alcohol extraction and water precipitation. The alcohol extraction and water precipitation method is also called alcohol water method, and is characterized in that ethanol with a certain concentration is mixed with medicinal materials for primary extraction, and then water is added into the obtained extracting solution to remove precipitates so as to obtain target components. Tannin and the like are extracted by 40 to 50 percent of ethanol; extracting glycosides with 60-70% ethanol; extracting alkaloid, volatile oil, resin and chlorophyll with ethanol of higher concentration. For the sake of clarity, it is noted that: the rhodiola root is root and whole grass of sedum gillin of Crassulaceae, and the name of the latin plant animal mineral is as follows: sedum middedorffianum Maxim. The Physcomitrella patens is Physcomitrella patens (Gunn.) Limpr of Physcomitrella, and is used as a whole herb.
The proportion of the traditional Chinese medicine compound medicine is optimized as follows: preferably, the traditional Chinese medicinal materials comprise the following components in parts by weight: 90 parts of honeysuckle, 90 parts of rhodiola root, 30 parts of clematis root and 3-7 parts of pansy.
In a preferred scheme, the Chinese medicinal materials comprise the following components in parts by weight: 90 parts of honeysuckle, 90 parts of rhodiola root, 30 parts of clematis root and 3 parts of pansy.
In another preferred scheme, the Chinese medicinal materials have the following weight parts: 90 parts of honeysuckle, 90 parts of rhodiola root, 30 parts of clematis root and 4 parts of panophytum planicum.
In another preferred scheme, the Chinese medicinal materials have the following weight parts: 90 parts of honeysuckle, 90 parts of rhodiola root, 30 parts of clematis root and 6 parts of panophytum planicum.
In another preferred scheme, the Chinese medicinal materials comprise the following components in parts by weight: 90 parts of honeysuckle, 90 parts of rhodiola root, 30 parts of clematis root and 7 parts of pansy.
The traditional Chinese medicine compound medicine for treating rheumatoid arthritis is an oral solid medicine or an oral liquid medicine.
Preferably, the traditional Chinese medicine compound medicine for treating rheumatoid arthritis is oral granules, and excipients of the oral granules comprise starch and microcrystalline cellulose. The preparation method of the traditional Chinese medicine compound medicine for treating rheumatoid arthritis comprises the following steps:
(1) respectively pulverizing flos Lonicerae, radix Rhodiolae, radix Clematidis and Physcomitrella, sieving with 60 mesh sieve, and mixing to obtain Chinese medicinal composition
Powder material;
(2) adding the Chinese medicinal material powder obtained in the step (1) into the Chinese medicinal material powder according to the ratio of the material to the liquid of 1:10 or 1:15
Soaking in 80% ethanol for 2 hr, extracting at 60 deg.C for 2 hr, filtering, and collecting the ethanol extract;
(3) concentrating the alcohol extract obtained in the step (2) under reduced pressure to obtain an extract with the relative density of 1.1-1.2, and adding the weight of the extract
Stirring 20 times of water, standing for 16-20 hours, filtering and collecting filtrate;
(4) concentrating the filtrate obtained in the step (3) to an extract with the relative density of 1.2-1.3, and then drying in vacuum with the vacuum degree
Drying at 50 deg.C under 0.09 MPa for 48 hr to obtain dry extract;
(5) crushing the dry extract obtained in the step (4), and sieving by a 80-mesh sieve to obtain dry extract powder; mixing the dry extract powder with starch and microcrystalline cellulose, wherein the dosage of the starch is 0.6 times of the weight of the dry extract powder, and the dosage of the microcrystalline cellulose is 0.4 times of the weight of the dry extract powder; and then adding ethanol with the volume concentration of 80% to prepare a soft material, sieving wet particles with a 16-mesh sieve, drying at 60 ℃, and then sieving with a 14-mesh sieve to complete the particles.
Naturally, the filtrate obtained in the step (3) can be used as an oral liquid preparation after being concentrated into an extract with the relative density of 1.2 to 1.3, or can be added into an auxiliary material port of the oral liquid preparation to prepare the oral liquid preparation.
The above ratio of material to liquid refers to the ratio of the weight of solid medicine as material to the volume of extraction reagent as liquid, and the unit of the ratio of material to liquid is g: ml in general, that is, 1g of Chinese medicinal powder corresponds to a certain ml of extraction reagent. If the material-liquid ratio is 1:10, 1g of Chinese medicinal powder is added with 10ml of extraction reagent. The above-mentioned volume concentration is also referred to as a volume fraction.
The traditional Chinese medicine compound medicine has a good effect on rheumatoid arthritis, and is simple in formula and high in safety.
Detailed Description
The following examples are provided to further illustrate the compound Chinese medicine of the present invention, its preparation and use.
Example 1 Compound Chinese medicine of honeysuckle and Clematis chinensis
The formula is as follows: 9000g of honeysuckle flower, 3000g of clematis root
Preparation:
(1) taking honeysuckle and clematis root according to the prescription amount, respectively crushing, sieving by a 60-mesh sieve, and mixing to obtain traditional Chinese medicine powder;
(2) adding the volume concentration of the traditional Chinese medicine powder obtained in the step (1) into the traditional Chinese medicine powder according to the material-liquid ratio of 1:10
Soaking in 80% ethanol for 2 hr, extracting at 60 deg.C for 2 hr, filtering, and collecting the ethanol extract;
(3) concentrating the alcohol extract obtained in the step (2) under reduced pressure to obtain an extract with the relative density of 1.2, adding water with the weight of 20 times that of the extract, stirring, standing for 20 hours, filtering and collecting filtrate;
(4) concentrating the filtrate obtained in the step (3) to obtain an extract with the relative density of 1.3, and then drying in vacuum with the vacuum degree of
Drying at 50 deg.C under 0.09 MPa for 48 hr to obtain dry extract;
(5) crushing the dry extract obtained in the step (4), and sieving by a 80-mesh sieve to obtain dry extract powder; mixing the dry extract powder with starch and microcrystalline cellulose, wherein the dosage of the starch is 0.6 times of the weight of the dry extract powder, and the dosage of the microcrystalline cellulose is 0.4 times of the weight of the dry extract powder; and then adding ethanol with the volume concentration of 80% to prepare a soft material, sieving wet particles with a 16-mesh sieve, drying at 60 ℃, and then sieving with a 14-mesh sieve to complete the particles.
Example 2 Compound Chinese medicine of honeysuckle, rhodiola root, Clematis chinensis and Physcomitrella
The formula is as follows: 9000g of honeysuckle, 9000g of rhodiola root, 3000g of clematis root and 300g of rhodobryum planum
Preparation:
(1) respectively crushing honeysuckle, rhodiola rosea, clematis root and rhodobryum planum according to the prescription amount, sieving by a 60-mesh sieve, and mixing to obtain traditional Chinese medicine powder;
(2) adding the volume concentration of the traditional Chinese medicine powder obtained in the step (1) into the traditional Chinese medicine powder according to the material-liquid ratio of 1:15
Soaking in 80% ethanol for 2 hr, extracting at 60 deg.C for 2 hr, filtering, and collecting the ethanol extract;
(3) concentrating the alcohol extract obtained in the step (2) under reduced pressure to obtain an extract with the relative density of 1.1, adding water with the weight of 20 times that of the extract, stirring, standing for 18 hours, filtering and collecting filtrate;
(4) concentrating the filtrate obtained in the step (3) to obtain an extract with the relative density of 1.2, and then drying in vacuum with the vacuum degree of
Drying at 50 deg.C under 0.09 MPa for 48 hr to obtain dry extract;
(5) crushing the dry extract obtained in the step (4), and sieving by a 80-mesh sieve to obtain dry extract powder; mixing the dry extract powder with starch and microcrystalline cellulose, wherein the dosage of the starch is 0.6 times of the weight of the dry extract powder, and the dosage of the microcrystalline cellulose is 0.4 times of the weight of the dry extract powder; and then adding ethanol with the volume concentration of 80% to prepare a soft material, sieving wet particles with a 16-mesh sieve, drying at 60 ℃, and then sieving with a 14-mesh sieve to complete the particles.
Example 3 Compound Chinese medicine of honeysuckle, rhodiola root, Clematis chinensis and Physcomitrella
The formula is as follows: 9000g of honeysuckle, 9000g of rhodiola root, 3000g of clematis root and 700g of rhodobryum planum
Preparation:
(1) respectively crushing honeysuckle, rhodiola rosea, clematis root and rhodobryum planum according to the prescription amount, sieving by a 60-mesh sieve, and mixing to obtain traditional Chinese medicine powder;
(2) adding the volume concentration of the traditional Chinese medicine powder obtained in the step (1) into the traditional Chinese medicine powder according to the material-liquid ratio of 1:15
Soaking in 80% ethanol for 2 hr, extracting at 60 deg.C for 2 hr, filtering, and collecting the ethanol extract;
(3) concentrating the alcohol extract obtained in the step (2) under reduced pressure to obtain an extract with the relative density of 1.1, adding water with the weight of 20 times that of the extract, stirring, standing for 16 hours, filtering and collecting filtrate;
(4) concentrating the filtrate obtained in the step (3) to obtain an extract with the relative density of 1.3, and then drying in vacuum with the vacuum degree of
Drying at 50 deg.C under 0.09 MPa for 48 hr to obtain dry extract;
(5) crushing the dry extract obtained in the step (4), and sieving by a 80-mesh sieve to obtain dry extract powder; mixing the dry extract powder with starch and microcrystalline cellulose, wherein the dosage of the starch is 0.6 times of the weight of the dry extract powder, and the dosage of the microcrystalline cellulose is 0.4 times of the weight of the dry extract powder; and then adding ethanol with the volume concentration of 80% to prepare a soft material, sieving wet particles with a 16-mesh sieve, drying at 60 ℃, and then sieving with a 14-mesh sieve to complete the particles.
Example 4 Compound Chinese medicine of honeysuckle, rhodiola root, Clematis chinensis and Physcomitrella
The formula is as follows: 9000g of honeysuckle, 9000g of rhodiola root, 3000g of clematis root and 400 g of rhodobryum planum
Preparation:
(1) respectively crushing honeysuckle, rhodiola rosea, clematis root and rhodobryum planum according to the prescription amount, sieving by a 60-mesh sieve, and mixing to obtain traditional Chinese medicine powder;
(2) adding the volume concentration of the traditional Chinese medicine powder obtained in the step (1) into the traditional Chinese medicine powder according to the material-liquid ratio of 1:15
Soaking in 80% ethanol for 2 hr, extracting at 60 deg.C for 2 hr, filtering, and collecting the ethanol extract;
(3) concentrating the alcohol extract obtained in the step (2) under reduced pressure to obtain an extract with the relative density of 1.2, adding water with the weight of 20 times that of the extract, stirring, standing for 20 hours, filtering and collecting filtrate;
(4) concentrating the filtrate obtained in the step (3) to obtain an extract with the relative density of 1.3, and then drying in vacuum with the vacuum degree of
Drying at 50 deg.C under 0.09 MPa for 48 hr to obtain dry extract;
(5) crushing the dry extract obtained in the step (4), and sieving by a 80-mesh sieve to obtain dry extract powder; mixing the dry extract powder with starch and microcrystalline cellulose, wherein the dosage of the starch is 0.6 times of the weight of the dry extract powder, and the dosage of the microcrystalline cellulose is 0.4 times of the weight of the dry extract powder; and then adding ethanol with the volume concentration of 80% to prepare a soft material, sieving wet particles with a 16-mesh sieve, drying at 60 ℃, and then sieving with a 14-mesh sieve to complete the particles.
Example 5 Compound Chinese medicine of honeysuckle, rhodiola root, Clematis chinensis and Physcomitrella
The formula is as follows: 9000g of honeysuckle, 9000g of rhodiola root, 3000g of clematis root and 600g of rhodobryum planum
Preparation:
(1) respectively crushing honeysuckle, rhodiola rosea, clematis root and rhodobryum planum according to the prescription amount, sieving by a 60-mesh sieve, and mixing to obtain traditional Chinese medicine powder;
(2) adding the volume concentration of the traditional Chinese medicine powder obtained in the step (1) into the traditional Chinese medicine powder according to the material-liquid ratio of 1:10
Soaking in 80% ethanol for 2 hr, extracting at 60 deg.C for 2 hr, filtering, and collecting the ethanol extract;
(3) concentrating the alcohol extract obtained in the step (2) under reduced pressure to obtain an extract with the relative density of 1.2, adding water with the weight of 20 times that of the extract, stirring, standing for 20 hours, filtering and collecting filtrate;
(4) concentrating the filtrate obtained in the step (3) to obtain an extract with the relative density of 1.3, and then drying in vacuum with the vacuum degree of
Drying at 50 deg.C under 0.09 MPa for 48 hr to obtain dry extract;
(5) crushing the dry extract obtained in the step (4), and sieving by a 80-mesh sieve to obtain dry extract powder; mixing the dry extract powder with starch and microcrystalline cellulose, wherein the dosage of the starch is 0.6 times of the weight of the dry extract powder, and the dosage of the microcrystalline cellulose is 0.4 times of the weight of the dry extract powder; and then adding ethanol with the volume concentration of 80% to prepare a soft material, sieving wet particles with a 16-mesh sieve, drying at 60 ℃, and then sieving with a 14-mesh sieve to complete the particles.
Example 6 drug efficacy against rheumatoid arthritis
Animal model, grouping, administration frequency and administration volume
Male SD rats weighing 180-200 g. 48 rats were taken and randomly divided into 8 groups of 6 rats each. Establishing a rheumatoid arthritis model by adopting a method of injecting Freund's complete adjuvant into foot plantaris. The injection site was left hind paw, injection volume was 0.1 mL. And establishing a rheumatoid arthritis model.
Each group of rats was dosed by gavage starting on day 8. The preparation is administered by intragastric administration 1 time per day, 1mL each time, and continuously for 4 weeks, namely 28 days. Before the first gavage, the thickness of the plantar aspect of the left hind paw of each rat was measured and counted as the thickness of the plantar aspect of the paw before the administration. The thickness of the plantar aspect of the left hind paw of each rat was again measured 8 hours after the last dose was completed and counted as the thickness of the plantar aspect of the hind paw after the dose.
Medicine and preparation
Except that 1 group was gavaged with saline as a model control group. The other groups are administered with different medicinal extracts by intragastric administration.
1. Positive control group:
simiaoyongan decoction reformulating formula is used as a positive control.
The formula is as follows: 900g of honeysuckle, 600g of figwort, 450g of angelica, 300g of liquorice and 300g of clematis root.
The preparation method comprises the following steps:
(1) taking honeysuckle, figwort, angelica, liquorice and clematis root according to the prescription amount, respectively crushing, sieving by a 60-mesh sieve, and mixing to obtain traditional Chinese medicine powder;
(2) adding the volume concentration of the traditional Chinese medicine powder obtained in the step (1) into the traditional Chinese medicine powder according to the material-liquid ratio of 1:10
Soaking in 80% ethanol for 2 hr, extracting at 60 deg.C for 2 hr, filtering, and collecting the ethanol extract;
(3) concentrating the alcohol extract obtained in the step (2) under reduced pressure to obtain an extract with the relative density of 1.2, adding water with the weight of 20 times that of the extract, stirring, standing for 20 hours, filtering and collecting filtrate;
(4) and (4) concentrating the filtrate obtained in the step (3) to obtain an extract with the relative density of 1.3.
2. Experimental drugs 1 group
The formula is as follows: 900g of honeysuckle and 300g of clematis root.
Preparation:
(1) taking honeysuckle and clematis root according to the prescription amount, respectively crushing, sieving by a 60-mesh sieve, and mixing to obtain traditional Chinese medicine powder;
(2) adding the volume concentration of the traditional Chinese medicine powder obtained in the step (1) into the traditional Chinese medicine powder according to the material-liquid ratio of 1:10
Soaking in 80% ethanol for 2 hr, extracting at 60 deg.C for 2 hr, filtering, and collecting the ethanol extract;
(3) concentrating the alcohol extract obtained in the step (2) under reduced pressure to obtain an extract with the relative density of 1.2, adding water with the weight of 20 times that of the extract, stirring, standing for 20 hours, filtering and collecting filtrate;
(4) and (4) concentrating the filtrate obtained in the step (3) to obtain an extract with the relative density of 1.3.
3. Experimental drugs 2 groups
The formula is as follows: rhodiola root 900 g.
Preparation:
(1) taking rhodiola root of a prescription amount, crushing, and sieving by a 60-mesh sieve to obtain Chinese medicinal material powder;
(2) adding the volume concentration of the traditional Chinese medicine powder obtained in the step (1) into the traditional Chinese medicine powder according to the material-liquid ratio of 1:10
Soaking in 80% ethanol for 2 hr, extracting at 60 deg.C for 2 hr, filtering, and collecting the ethanol extract;
(3) concentrating the ethanol extract obtained in step (2) under reduced pressure to obtain extract with relative density of 1.2, and adding 20 times of the extract
Water, stirring, standing for 20 hours, filtering and collecting filtrate;
(4) and (4) concentrating the filtrate obtained in the step (3) to obtain an extract with the relative density of 1.3.
4. Experimental drugs 3 groups
The formula is as follows: 400 g of sphagnum planiculum.
Preparation:
(1) taking a prescription amount of the sphagnum planiculum, crushing, and sieving by a 60-mesh sieve to obtain traditional Chinese medicine powder;
(2) adding the volume concentration of the traditional Chinese medicine powder obtained in the step (1) into the traditional Chinese medicine powder according to the material-liquid ratio of 1:10
Soaking in 80% ethanol for 2 hr, extracting at 60 deg.C for 2 hr, filtering, and collecting the ethanol extract;
(3) concentrating the alcohol extract obtained in the step (2) under reduced pressure to obtain an extract with the relative density of 1.2, adding water with the weight of 20 times that of the extract, stirring, standing for 20 hours, filtering and collecting filtrate;
(4) and (4) concentrating the filtrate obtained in the step (3) to obtain an extract with the relative density of 1.3.
5. Experimental drugs 4 groups
The formula is as follows: 900g of honeysuckle, 900g of rhodiola root and 300g of clematis root.
Preparation:
(1) taking honeysuckle, rhodiola root and clematis root according to the prescription amount, respectively crushing, sieving by a 60-mesh sieve, and mixing to obtain traditional Chinese medicine powder;
(2) adding the volume concentration of the traditional Chinese medicine powder obtained in the step (1) into the traditional Chinese medicine powder according to the material-liquid ratio of 1:10
Soaking in 80% ethanol for 2 hr, extracting at 60 deg.C for 2 hr, filtering, and collecting the ethanol extract;
(3) concentrating the alcohol extract obtained in the step (2) under reduced pressure to obtain an extract with the relative density of 1.2, adding water with the weight of 20 times that of the extract, stirring, standing for 20 hours, filtering and collecting filtrate;
(4) and (4) concentrating the filtrate obtained in the step (3) to obtain an extract with the relative density of 1.3.
6. Experimental drugs 5 groups
The formula is as follows: 900g of honeysuckle, 300g of clematis root and 40 g of sphagnum planiculum.
Preparation:
(1) taking honeysuckle, clematis root and Physcomitrella on prescription dose, respectively crushing, sieving by a 60-mesh sieve, and mixing to obtain traditional Chinese medicine powder;
(2) adding the volume concentration of the traditional Chinese medicine powder obtained in the step (1) into the traditional Chinese medicine powder according to the material-liquid ratio of 1:10
Soaking in 80% ethanol for 2 hr, extracting at 60 deg.C for 2 hr, filtering, and collecting the ethanol extract;
(3) concentrating the alcohol extract obtained in the step (2) under reduced pressure to obtain an extract with the relative density of 1.2, adding water with the weight of 20 times that of the extract, stirring, standing for 20 hours, filtering and collecting filtrate;
(4) and (4) concentrating the filtrate obtained in the step (3) to obtain an extract with the relative density of 1.3.
Experimental drugs 6 groups
The formula is as follows: 900g of honeysuckle, 900g of rhodiola root, 300g of clematis root and 40 g of rhodobryum planum.
Preparation:
(1) respectively crushing honeysuckle, rhodiola rosea, clematis root and rhodobryum planum according to the prescription amount, sieving by a 60-mesh sieve, and mixing to obtain traditional Chinese medicine powder;
(2) adding the volume concentration of the traditional Chinese medicine powder obtained in the step (1) into the traditional Chinese medicine powder according to the material-liquid ratio of 1:10
Soaking in 80% ethanol for 2 hr, extracting at 60 deg.C for 2 hr, filtering, and collecting the ethanol extract;
(3) concentrating the alcohol extract obtained in the step (2) under reduced pressure to obtain an extract with the relative density of 1.2, adding water with the weight of 20 times that of the extract, stirring, standing for 20 hours, filtering and collecting filtrate;
(4) and (4) concentrating the filtrate obtained in the step (3) to obtain an extract with the relative density of 1.3.
Third, experimental results and conclusions
The results of measurements and statistics of the thickness of the plantar aspect of the feet before and after administration for each group of rats are shown in the following table.
a: p <0.01 compared to model group; b: p <0.01 compared to positive control group; and c, compared with the experimental drug 1 group, P is less than 0.01.
Comparison between groups showed no significant difference in plantar thickness in the animals of each group prior to dosing (p > 0.05). Statistical analysis between groups was performed on the thickness of the plantar aspect of each group of animals after dosing. The results show that the thickness of the plantar of the 6 groups of the positive control and the experimental drug is obviously smaller than that of the model group (p is less than 0.01), which indicates that the two groups have obvious inhibition effect on the rheumatoid arthritis. The thickness of the foot sole of the experimental medicine 1, the experimental medicine 4 and the experimental medicine 5 is obviously smaller than that of the model group, the difference is not obvious, but the P value is close to 0.06, which indicates that the experimental medicine 1, the experimental medicine 4 and the experimental medicine 5 have certain inhibition effect on the rheumatoid arthritis, but are not ideal. The thickness of the plantar of the experimental medicine 2 and the experimental medicine 3 groups is not obviously different from that of the model group, and the thickness of the plantar of the foot of the experimental medicine 3 groups has no inhibition effect on the rheumatoid arthritis.
The thickness of the foot sole of the experimental drug 6 groups is obviously smaller than that of the positive control group and the experimental drug 1 group (p is less than 0.01), which shows that the inhibition effect of the experimental drug 6 groups on the rheumatoid arthritis is better than that of the positive control group and the experimental drug 1.
Claims (8)
1. The traditional Chinese medicine compound medicine for treating rheumatoid arthritis is characterized by being prepared from the following traditional Chinese medicines: honeysuckle, rhodiola root, clematis root and sphagnum planiculosum; the traditional Chinese medicinal materials are extracted by an alcohol extraction and water precipitation method, and the traditional Chinese medicinal materials comprise the following components in parts by weight: 90 parts of honeysuckle, 90 parts of rhodiola root, 30 parts of clematis root and 3-7 parts of pansy.
2. The traditional Chinese medicine compound medicine for treating rheumatoid arthritis according to claim 1, wherein the traditional Chinese medicine comprises the following traditional Chinese medicine materials in parts by weight: 90 parts of honeysuckle, 90 parts of rhodiola root, 30 parts of clematis root and 3 parts of pansy.
3. The traditional Chinese medicine compound medicine for treating rheumatoid arthritis according to claim 1, wherein the traditional Chinese medicine comprises the following traditional Chinese medicine materials in parts by weight: 90 parts of honeysuckle, 90 parts of rhodiola root, 30 parts of clematis root and 4 parts of panophytum planicum.
4. The traditional Chinese medicine compound medicine for treating rheumatoid arthritis according to claim 1, wherein the traditional Chinese medicine comprises the following traditional Chinese medicine materials in parts by weight: 90 parts of honeysuckle, 90 parts of rhodiola root, 30 parts of clematis root and 6 parts of panophytum planicum.
5. The traditional Chinese medicine compound medicine for treating rheumatoid arthritis according to claim 1, wherein the traditional Chinese medicine comprises the following traditional Chinese medicine materials in parts by weight: 90 parts of honeysuckle, 90 parts of rhodiola root, 30 parts of clematis root and 7 parts of pansy.
6. The compound traditional Chinese medicine for treating rheumatoid arthritis according to claim 1, wherein the compound traditional Chinese medicine for treating rheumatoid arthritis is an oral solid medicine.
7. The compound traditional Chinese medicine for treating rheumatoid arthritis according to claim 6, wherein the compound traditional Chinese medicine for treating rheumatoid arthritis is an oral granule, and the excipient of the oral granule comprises starch and microcrystalline cellulose.
8. The preparation method of the traditional Chinese medicine compound medicine for treating rheumatoid arthritis according to claim 7, which is characterized by comprising the following steps:
s1, respectively pulverizing flos Lonicerae, radix Rhodiolae, radix Clematidis and Physcomitrella, sieving with 60 mesh sieve, and mixing to obtain Chinese medicinal powder;
s2, adding 80 vol% ethanol into the Chinese medicinal powder obtained in the step S1 according to the ratio of the materials to the liquid of 1:10 or 1:15, soaking for 2 hours, extracting for 2 hours at 60 ℃, filtering and collecting an ethanol extract;
s3, concentrating the alcohol extract obtained in the step S2 under reduced pressure to obtain an extract with the relative density of 1.1-1.2, adding water with the weight of 20 times that of the extract, stirring, standing for 16-20 hours, filtering and collecting filtrate;
s4, concentrating the filtrate obtained in the step S3 to an extract with the relative density of 1.2-1.3, and then drying in vacuum at the vacuum degree of 0.09 MPa and the drying temperature of 50 ℃ for 48 hours to obtain a dry extract;
s5: crushing the dry extract obtained in the step S4, and sieving the crushed dry extract with a 80-mesh sieve to obtain dry extract powder; mixing the dry extract powder with starch and microcrystalline cellulose, wherein the dosage of the starch is 0.6 times of the weight of the dry extract powder, and the dosage of the microcrystalline cellulose is 0.4 times of the weight of the dry extract powder; and then adding ethanol with the volume concentration of 80% to prepare a soft material, sieving wet particles with a 16-mesh sieve, drying at 60 ℃, and then sieving with a 14-mesh sieve to complete the particles.
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Citations (4)
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|---|---|---|---|---|
| CN1236638A (en) * | 1999-06-11 | 1999-12-01 | 王建明 | Medical wine for treating rheumatism |
| CN1628810A (en) * | 2004-09-13 | 2005-06-22 | 徐志林 | Black ants and Chinese clematis containing capsule |
| CN102772507A (en) * | 2012-06-29 | 2012-11-14 | 郝素娥 | Rheumatism removing medicinal liquor for women |
| CN104825566A (en) * | 2015-04-15 | 2015-08-12 | 昆明市中医医院 | Bee needle attenuation and effect-increase oral liquid and preparation method thereof |
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2019
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1236638A (en) * | 1999-06-11 | 1999-12-01 | 王建明 | Medical wine for treating rheumatism |
| CN1628810A (en) * | 2004-09-13 | 2005-06-22 | 徐志林 | Black ants and Chinese clematis containing capsule |
| CN102772507A (en) * | 2012-06-29 | 2012-11-14 | 郝素娥 | Rheumatism removing medicinal liquor for women |
| CN104825566A (en) * | 2015-04-15 | 2015-08-12 | 昆明市中医医院 | Bee needle attenuation and effect-increase oral liquid and preparation method thereof |
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| 清热活血通痹汤治疗活动期类风湿性关节炎30例;陈军梅;《陕西中医》;20070831;第28卷(第8期);第1009-1010页 * |
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