CN109699444A - A kind of Flos micheliae Albae matches the preparation method of the raw-soil - Google Patents
A kind of Flos micheliae Albae matches the preparation method of the raw-soil Download PDFInfo
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- CN109699444A CN109699444A CN201910188634.7A CN201910188634A CN109699444A CN 109699444 A CN109699444 A CN 109699444A CN 201910188634 A CN201910188634 A CN 201910188634A CN 109699444 A CN109699444 A CN 109699444A
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- flos micheliae
- micheliae albae
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Abstract
The present invention provides the preparation methods that a kind of Flos micheliae Albae matches the raw-soil, and include the steps that one expands culture mycorrhizal fungi: by the short and small umbrella branch of mycorrhizal fungi, mould, sclerotium aspergillus and Glomus mosseae carry out expanding respectively numerous, obtain liquid bacterium solution；The step of one preparation garden waste composting production；By the crushed material composting of garden waste, form plant growing medium, by short and small umbrella branch, the liquid bacterium solution of mould, sclerotium aspergillus and Glomus mosseae mix with the composting production of garden waste so that living bacteria count be not less than 0.2 hundred million/gram；The afforestation castoff compost product for being added to mould short and small umbrella branch, sclerotium aspergillus and Glomus mosseae is uniformly mixed with original soil, while adding river sand, Flos micheliae Albae is formed and matches the raw-soil.The present invention creates the preparation method that a kind of Flos micheliae Albae matches the raw-soil using Mycorrhizal Technology, can obtain a kind of Flos micheliae Albae in a relatively short period of time with the raw-soil, can rapidly promote Flos micheliae Albae growth.
Description
Technical field
The invention belongs to landscape architecture fields, are related to a kind of soil, and specifically a kind of Flos micheliae Albae matches the preparation side of the raw-soil
Method.
Background technique
Flos micheliae Albae is Magnoliales, Magnoliaceae, Magnolia deciduous tree, and alias Flos micheliae Albae hopes spring Flos micheliae Albae, white jade orchid,
For Magnoliaceae Magnolia Flos micheliae Albae subgenus deciduous tree, there are the kinds such as Flos micheliae Albae, purple Flos micheliae Albae, two tall Flos micheliae Albaes, non-irrigated lotus Flos micheliae Albae
(kind), Chinese Famous flowers and trees, early spring important flowering tree have more than 2,000 years cultivation histories, and Flos micheliae Albae happiness is warm wet
Moisten weather and fertile chesson, not drought-resistant and waterlogging, the ground such as source area Shaanxi, Gansu, Henan, Hubei, Sichuan.Due to white
The trunk of yulan is smooth, and tree-like grace, pattern is plain, and strong flavor fragrance, ornamental value is high, and now many cities carry out introducing a fine variety cultivation
Urban greening species are enriched in training, promote garden landscape.Since different cities soil characteristic and Flos micheliae Albae source area soil have
Larger difference, different cities Flos micheliae Albae are introduced a fine variety there are certain difficulty, be badly in need of it is a kind of rapidly promote Flos micheliae Albae survival and growth match
The raw-soil.
Refer to that human intervention is made and meets the soil of plant health fast-growth condition with the raw-soil.With traditional planting technique
It is native different, with the raw-soil on the basis ofs focusing on soil texture, nutrition, safety etc., more emphasis biological activity of soil and its promotion.Bacterium
Root is the mutualism body of the mycorrhizal fungi and root system of plant formation in soil, a kind of generally existing symbiosis of nature.
Mould short and small umbrella branch is the mould category strain of Zygomycotina Mucoales umbrella shape, and bacterium colony front white, mycelia is fine and close, is attached to
Media surface, reverse side white to faint yellow, a little pigment deposition.Sclerotium aspergillus is Deuteromycotina shell Mei Mubeimei section bacterium
Kind, bacterium colony yellow to brown, quality velvet shape generates a large amount of sclerotium, and sclerotium is mostly spherical or subsphaeroidal.
Glomus mosseae be the mould section's Paraglomus strain of Zygomycota Glomales sacculus, mycelia yellow green to yellowish-brown,
Cystocarp is irregular spherical shape, includes 1 to multiple spores, spore is pale yellow to yellowish-brown.
Summary of the invention
The purpose of the present invention is to provide the preparation method that a kind of Flos micheliae Albae matches the raw-soil, this Flos micheliae Albae matches the raw-soil
Preparation method to solve each city in non-Flos micheliae Albae source area in the prior art soil be not suitable for Flos micheliae Albae growth skill
Art problem.
The present invention provides the preparation methods that a kind of Flos micheliae Albae matches the raw-soil, include the following steps:
1) mycorrhizal fungi is expanded culture the step of: mould, sclerotium aspergillus and Moses's ball by the short and small umbrella branch of mycorrhizal fungi
Capsule is mould to carry out expanding respectively numerous, and expansion breeding culture medium is liquid MMN culture medium, and the liquid MMN culture medium is by calcium chloride, chlorination
Sodium, potassium dihydrogen phosphate, diammonium hydrogen phosphate, epsom salt, ferric trichloride, vitamin B1, malt extract, glucose, distillation
Water composition, the calcium chloride, sodium chloride, potassium dihydrogen phosphate, diammonium hydrogen phosphate, epsom salt, ferric trichloride, vitamin B1
, malt extract, glucose, distilled water material ratio be 0.05g:0.025g:0.5g:0.25g:0.15g:10g:0.1mg:
2g:15g:1000ml；Any one mycorrhizal fungi fungi shaken cultivation 5 ~ 7 under conditions of 25 DEG C, 120 ~ 150r/min of revolving speed
It, obtains liquid bacterium solution；
2) the step of crushed material of a preparation garden waste, by the arbor after being trimmed during afforestation, shrub, draft
Any one in waste or two or more material mixings, being ground into partial size is 5cm particle below；
3) a step of addition mycorrhizal fungi adds, by the crushed material of the garden waste of step 2 carry out moisture regulation to 60% ~
70%, composting is under 26 ~ 30 DEG C of environment, and turning is primary weekly, 20 ~ 30 days formation plant growing mediums, step 1) is obtained short
Umbellule branch is mould, any one or two or more any combination in the liquid bacterium solution of sclerotium aspergillus and Glomus mosseae and garden
Woods castoff compost product mix so that living bacteria count not less than 0.2 hundred million/gram；
4) by step 3) be added to the afforestation castoff compost product of mould short and small umbrella branch, sclerotium aspergillus and Glomus mosseae with
Original soil is uniformly mixed, and the ratio of addition is the 30% ~ 50% of original soil volume, while adding river sand, and the ratio of river sand addition is original soil
The 5% ~ 10% of volume forms Flos micheliae Albae and matches the raw-soil.
Further, if original soil is saline-alkali soil, then gypsum is added, the ratio of the addition of gypsum is original soil volume
0.04%~0.08%。
Specifically, short and small umbrella branch is mould, sclerotium aspergillus and Glomus mosseae are conventional bacterial classification, those skilled in the art is equal
Understand its physicochemical property.
Short and small umbrella branch is mould, sclerotium aspergillus strain can be ordered from China General Microbiological culture presevation administrative center.It is short and small
The mould CGMCC deposit number of umbrella branch is 5.826,3.16343,3.16316,3.16311 or 5.707.The CGMCC of sclerotium aspergillus is protected
Hiding number is 3.13914,3.13617,3.7160,3.135,3.6353,3.6352,3.2578 or 3.86；
Short and small umbrella branch is mould, sclerotium aspergillus strain can be inquired from Chinese microorganism strain net (http://www.biobw.org/,
The Tel:010-51291224 E-mail:biobw11@sina.com of Zaojia Street, Fengtai District, Beijing City 11) purchase；It is " short and small
Umbrella branch is mould " platform number be bio-08775；The platform number of sclerotium aspergillus is bio-089198, bio-088835, bio-
12940, bio-12563, bio-12560, bio-12557 or bio-12539.
Glomus mosseae can buy (https: //china.guidechem.com/trade/ from Gaede chemical industry online shopping
Pdetail7933220.html), specific supplier is Shanghai Yan Sheng Industrial Co., Ltd..
The present inventor is by for a long time having found Flos micheliae Albae root soil microbe research, soil around Flos micheliae Albae root system
Earth is dispersed with the mycorrhizal fungis types such as mould a large amount of short and small umbrella branch, sclerotium aspergillus, Glomus mosseae, and mould short and small umbrella branch is zygomycete
The mould category strain of subphylum Mucoales umbrella shape, colonizes on the root system of a small number of plants, and short and small umbrella branch mould mycorrhiza fungi can expand plant
Surface area of root systern improves absorption of the plant to soil nutrient and moisture；Sclerotium aspergillus mycorrhizal fungi and plant form syntaxial system, mutually
Favour is mutually beneficial, common evolutionary；Glomus mosseae promote photosynthesis of plant, anti-stress, it is disease-resistant, promote plant to the absorption of phosphorus and
There is important role in terms of improvement soil.Different floristics rhizospheres are dispersed with specific mycorrhizal fungi type, the present invention due to
It is added to the mould short and small umbrella branch of Flos micheliae Albae mycorrhizal fungi, sclerotium aspergillus and Glomus mosseae, by the distinctive mycorrhizal fungi class of Flos micheliae Albae
Group is applied to Flos micheliae Albae and researches and develops with the raw-soil, can promote the formation of Flos micheliae Albae root mycorhiza structure, to promote the life of Flos micheliae Albae
It is long.
The present invention is compared with prior art, its technical effect is that actively and apparent.The present invention is created using Mycorrhizal Technology
A kind of Flos micheliae Albae matches the preparation method of the raw-soil.Method of the invention is convenient and efficient, can obtain in a relatively short period of time a kind of white
Yulan matches the raw-soil, can rapidly promote Flos micheliae Albae growth, does not furthermore need expensive instrument and equipment preparation.The present invention is to Flos micheliae Albae
Play a significant role in different zones successful introduction with cultivation, while also being mentioned for the excellent tree species of other city trees and shrubs and flower cultivation
It is used for reference for method.The present invention can reduce urban resource waste and environmental pollution with the research and development of the raw-soil, increase soil fertility, effective
It improves Flos micheliae Albae survival rate, promote Flos micheliae Albae growing way, should have reference value with raw-soil research and development to other specified plants with the raw-soil,
It is suitable for popularization and application.
Specific embodiment
Embodiment 1
The present invention provides the preparation methods that a kind of Flos micheliae Albae matches the raw-soil, include the following steps:
1) mycorrhizal fungi is expanded culture the step of: mould, sclerotium aspergillus and Moses's ball by the short and small umbrella branch of mycorrhizal fungi
Capsule is mould to carry out expanding respectively numerous, and expansion breeding culture medium is liquid MMN culture medium, has calcium chloride 0.05g, sodium chloride in MMN culture medium
0.025g, potassium dihydrogen phosphate 0.5g, diammonium hydrogen phosphate 0.25g, epsom salt 0.15g, ferric sesquichloride 10g, vitamin B1
0.1mg, malt extract 2g, glucose 15g, distilled water 1000ml；Any one mycorrhizal fungi fungi 25 DEG C, revolving speed 120 ~
Shaken cultivation 5 ~ 7 days under conditions of 150r/min obtain liquid bacterium solution；
2) the step of preparation garden waste composting production, by the arbor after being trimmed during afforestation, shrub, draft
Any one in waste or two or more material mixings, being ground into partial size is 5cm particle below, and gardens are given up
The crushed material of gurry carries out moisture regulation to 60% ~ 70%, and composting is under 26 ~ 30 DEG C of environment, and turning is primary weekly, 20 ~ 30 days shapes
At plant growing medium；
3) a step of addition mycorrhizal fungi adds, mould, sclerotium aspergillus and Glomus mosseae by the short and small umbrella branch of step 1) acquisition
Liquid bacterium solution in any one or two or more any combination mixed with garden waste composting production so that effectively
Viable count not less than 0.2 hundred million/gram；
4) by step 3) be added to the afforestation castoff compost product of mould short and small umbrella branch, sclerotium aspergillus and Glomus mosseae with
Original soil is uniformly mixed, and the ratio of addition is the 30% ~ 50% of original soil volume, while adding river sand, and the ratio of river sand addition is original soil
The 5% ~ 10% of volume forms Flos micheliae Albae and matches the raw-soil.
Further, if original soil is saline-alkali soil, then gypsum is added, the ratio of the addition of gypsum is original soil volume
0.04%~0.08%。
Embodiment 2
The present invention provides the preparation methods that a kind of Flos micheliae Albae matches the raw-soil, include the following steps:
1) mycorrhizal fungi is expanded culture the step of: mould, sclerotium aspergillus and Moses's ball by the short and small umbrella branch of mycorrhizal fungi
Capsule is mould to carry out expanding respectively numerous, and expansion breeding culture medium is liquid MMN culture medium, has calcium chloride 0.05g, sodium chloride in MMN culture medium
0.025g, potassium dihydrogen phosphate 0.5g, diammonium hydrogen phosphate 0.25g, epsom salt 0.15g, ferric sesquichloride 10g, vitamin B1
0.1mg, malt extract 2g, glucose 15g, distilled water 1000ml；Any one mycorrhizal fungi fungi 25 DEG C, revolving speed 120 ~
Shaken cultivation 5 ~ 7 days under conditions of 150r/min obtain liquid bacterium solution；
2) the step of preparation garden waste composting production, by the arbor after being trimmed during afforestation, shrub, draft
Any one in waste or two or more material mixings, being ground into partial size is 5cm particle below, and gardens are given up
The crushed material of gurry carries out moisture regulation to 60% ~ 70%, and composting is under 26 ~ 30 DEG C of environment, and turning is primary weekly, 20 ~ 30 days shapes
At plant growing medium；
3) a step of addition mycorrhizal fungi adds, mould, sclerotium aspergillus and Glomus mosseae by the short and small umbrella branch of step 1) acquisition
Liquid bacterium solution according to volume ratio be 1:1:1 combination mixed with garden waste composting production, after adding liquid bacterium solution so that
Living bacteria count not less than 0.2 hundred million/gram；
4) by step 3) be added to the afforestation castoff compost product of mould short and small umbrella branch, sclerotium aspergillus and Glomus mosseae with
Original soil is uniformly mixed, and the ratio of addition is the 40% of original soil volume, while adding river sand, and the ratio of river sand addition is original soil volume
10%, formed Flos micheliae Albae match the raw-soil.
Embodiment 3
Basin is filled with the raw-soil using what embodiment 2 prepared, control group is original soil.It is the implantation of 1-2cm or so Flos micheliae Albae seedling by the diameter of a cross-section of a tree trunk 1.3 meters above the ground
It tests in basin, is irrigated with water.Later period normally conserves.Record a plant growth data, including height of seedling, the diameter of a cross-section of a tree trunk 1.3 meters above the ground, hat every two weeks later
Width.Growth result is counted after 1 year, test group seedling increases by the 28%, diameter of a cross-section of a tree trunk 1.3 meters above the ground compared with control group height of seedling and increases by 32%, hat width increase by 53%.
Claims (2)
1. the preparation method that a kind of Flos micheliae Albae matches the raw-soil, it is characterised in that include the following steps:
1) mycorrhizal fungi is expanded culture the step of: mould, sclerotium aspergillus and Moses's ball by the short and small umbrella branch of mycorrhizal fungi
Capsule is mould to carry out expanding respectively numerous, and expansion breeding culture medium is liquid MMN culture medium, and the liquid MMN culture medium is by calcium chloride, chlorination
Sodium, potassium dihydrogen phosphate, diammonium hydrogen phosphate, epsom salt, ferric trichloride, vitamin B1, malt extract, glucose, distillation
Water composition, the calcium chloride, sodium chloride, potassium dihydrogen phosphate, diammonium hydrogen phosphate, epsom salt, ferric trichloride, vitamin
B1, malt extract, glucose, distilled water material ratio be 0.05g:0.025g:0.5g:0.25g:0.15g:10g:0.1mg:
2g:15g:1000ml；Any one mycorrhizal fungi fungi under conditions of 25 DEG C, 120~150r/min of revolving speed shaken cultivation 5~
7 days, obtain liquid bacterium solution；
2) the step of preparation garden waste composting production, by the arbor after being trimmed during afforestation, shrub, draft
Any one in waste or two or more material mixings, being ground into partial size is 5cm particle below, by crushed material
Moisture regulation is carried out to 60%~70%, composting is under 26~30 DEG C of environment, and turning is primary weekly, and formation plant is planted within 20~30 days
Train medium；
3) a step of addition mycorrhizal fungi adds, mould, sclerotium aspergillus and Glomus mosseae by the short and small umbrella branch of step 1) acquisition
Liquid bacterium solution in any one or two or more any combination mixed with garden waste composting production so that effectively
Viable count not less than 0.2 hundred million/gram；
4) by step 3) be added to the afforestation castoff compost product of mould short and small umbrella branch, sclerotium aspergillus and Glomus mosseae with
Original soil is uniformly mixed, and the ratio of addition is the 30%~50% of original soil volume, while adding river sand, and the ratio of river sand addition is original
The 5%~10% of soil body product forms Flos micheliae Albae and matches the raw-soil.
2. the preparation method that a kind of Flos micheliae Albae described in claim 1 matches the raw-soil, it is characterised in that: match in the raw-soil also in described
Gypsum is added, the ratio of the addition of the gypsum is the 0.04%~0.08% of original soil volume.
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| CN108651127A (en) * | 2018-05-02 | 2018-10-16 | 合肥绿叶园林工程有限责任公司 | A method of planting Flos micheliae Albae using saline-alkali soil |
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2019
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| CN107043282A (en) * | 2016-02-05 | 2017-08-15 | 大汉酵素生物科技股份有限公司 | A kind of organic fertilizer for increasing crop trace element content and preparation method thereof |
| CN106278631A (en) * | 2016-08-18 | 2017-01-04 | 安庆市宝风农业发展有限公司 | A kind of Flos micheliae Albae growing nursery and culture substrate and preparation method thereof |
| CN108651127A (en) * | 2018-05-02 | 2018-10-16 | 合肥绿叶园林工程有限责任公司 | A method of planting Flos micheliae Albae using saline-alkali soil |
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