CN109679870B - A kind of biological organic fertilizer and preparation method thereof with water conservation drought resisting function - Google Patents
A kind of biological organic fertilizer and preparation method thereof with water conservation drought resisting function Download PDFInfo
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- CN109679870B CN109679870B CN201910011668.9A CN201910011668A CN109679870B CN 109679870 B CN109679870 B CN 109679870B CN 201910011668 A CN201910011668 A CN 201910011668A CN 109679870 B CN109679870 B CN 109679870B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/02—Other organic fertilisers from peat, brown coal, and similar vegetable deposits
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The present invention relates to agricultural biological technical fields, and in particular to a kind of biological organic fertilizer and preparation method thereof with water conservation drought resisting function.The biological organic fertilizer includes bacillus subtilis (Bacillus subtilis) GE3；Bacillus subtilis (Bacillus subtilis) the GE3 deposit number is CGMCC No.11966.The bacterial strain in drought environment not only have stronger colonization ability, and can also significantly improve forest in drought environment at rate, promote the growth of forest.The present invention is retained drought-resisting biological organic fertilizer using the solid drought resisting microbial inoculum containing bacillus subtilis GE3, fermentation washing cow dung as main component, add a small amount of water-retaining agent, each ingredient interaction, growth of the forest in drought environment can be greatly facilitated, the drought-resistant stress ability of forest is improved, survival rate of the forest in drought environment is improved.
Description
Technical field
The present invention relates to agricultural biological technical fields, and in particular to it is a kind of with water conservation drought resisting function biological organic fertilizer and
Preparation method.
Background technique
Biological organic fertilizer refers to microorganisms with specific functions and mainly with plant and animal residues (such as feces of livestock and poultry, agricultural crop straw
Deng) it is source and one kind for being combined through harmless treatment, decomposed organic materials has both microbial manure and organic fertilizer efficiency
The fertilizer answered.However, a large number of studies show that: the interaction of functional microorganism and crop is often and unstable, this unstable
Property derived from varying environment condition to microorganism growth impact, to restrict its effect.This requires in microbial manure
Functional microorganism adapts to external environment, could preferably play its effect.
Arid seriously threatens the growth, development and breeding of plant.Arid Problem also becomes the common problem in the whole world.The whole world
The area of Arid&semi-arid area accounts for about the 35% of land area, and the Arid&semi-arid area in China is vast in territory, is related to east
12 provinces, the autonomous region, city in north, northwest, North China, area account for about the 52.5% of national total area.In arid habitat, a large amount of micro- lifes
Object cannot be colonized successfully and be survived, and number of bacteria is caused to be greatly decreased, and structure of community diversity is substantially reduced, to influence to plant
Object rhizosphere soil growing environment influences the normal moisture of plant and Nutrient Absorption, reduces the drought-resistant ability of plant.This just needs to give birth to
Microorganism in organic fertilizer material adapts to the effect of Drought Stress could preferably play microbial manure.
The present inventor has filtered out one plant of walnut rhizosphere growth-promoting acinetobacter calcoaceticus X128 from walnut Rhizosphere sampling,
Deposit number is CGMCC No.7071, can be obviously promoted walnut growth and development, improves the growing environment of walnut rhizosphere soil.It should
Bacterial strain and its application have obtained national inventing patent authorization (CNCN103060241B).For Drought Stress, the present inventor is also screened
To one plant of bacillus cereus (Bacillus cereus) L90, which has also obtained invention patent protection
(CN103667132B)。
However, microbial resources are rich and varied in nature, the microbial resources for adapting to Drought Stress are further excavated, it is right
In application of the microbial manure in Drought Stress, improves forest drought tolerance and be of great significance.
Summary of the invention
Aiming at the problem that involved in the background technique, the present invention provides a kind of biological organic fertilizers with water conservation drought resisting function
And preparation method thereof.The biological organic fertilizer with water conservation drought resisting function of the present invention contains bacillus subtilis (Bacillus
Subtilis) GE3, the bacterial strain are not only applicable to drought environment, improve the drought tolerance of nursery stock；And the bacterial strain can also be shown
The stability for increasing biological organic fertilizer is write, keeps organic fertilizer fertility lasting.
The invention adopts the following technical scheme:
First aspect of the present invention provides a kind of bacillus subtilis, which is named as bacillus subtilis
It is common to be preserved in China Committee for Culture Collection of Microorganisms on January 7th, 2016 by (Bacillus subtilis) GE3
Microorganism center, deposit number are CGMCC No.11966.
The colony characteristics of the plant rhizosphere growth-promoting bacterium are as follows: GE3 bacterial strain bacterium colony on LB culture medium is rounded irregular,
Bacterium colony size is about 5mm for 24 hours for culture, and dry tack free tarnish edge is irregular, and bacterium colony is in brown color and intermediate color is deeper, no
It is transparent, there is gemma.
The second aspect of the present invention provides bacillus subtilis described above and is improving the application in plant drought ability.
Further, the plant is forest, preferably elder or honeysuckle.
Bacillus subtilis of the present invention acts in forest soil, can significantly promote the life of the forests such as elder, honeysuckle
Long and drought resistance.
Third aspect of the present invention provides a kind of drought resisting solid fungicide, by the micro- of activation humic acid and bacillus subtilis
Bio-fermented liquid mixes；Bacillus subtilis (Bacillus subtilis) the GE3 deposit number is CGMCC
No.11966；Drought resisting solid fungicide living bacteria count >=1 × 109cfu/g。
Further, the drought resisting solid fungicide by activation humic acid and includes bacillus subtilis (Bacillus
Subtilis) microbial fermentation solution of GE3 is mixed by solid-liquid ratio 5:1~2W/V；Bacillus subtilis (the Bacillus
Subtilis) GE3 deposit number is CGMCC No.11966.
Further, the preparation method of the activation humic acid is to press the nitric acid of Lignitic Humic Acid raw material and 20~25%
According to ratio 20~40min of hybrid reaction of 2:1, centrifugation falls nitric acid solution, tap water is then added, and is centrifuged, operates repeatedly, until from
Heart liquid pH > 5.5, drying.
It is a discovery of the invention that mixed using above-described humic acid with bacillus subtilis GE3, it not only can effective adhered bacteria
Body is conducive to being uniformly mixed for thallus and other ingredients, but also can protect the vigor of bacillus subtilis GE3, prevents from being granulated
Damage to thallus vigor, and can arid soil environment in, can advantageously promote bacillus subtilis GE3 colonize and
Breeding.Absorption carrier of the activation humic acid being prepared using the method for the present invention as bacillus subtilis GE3, effect is most
It is good.
The 4th aspect of the present invention, provides a kind of water conservation drought-resisting biological organic fertilizer, includes bacillus subtilis (Bacillus
subtilis)GE3；Bacillus subtilis (Bacillus subtilis) the GE3 deposit number is CGMCC No.11966.
Further, the water conservation drought-resisting biological organic fertilizer is pressed by solid drought resisting microbial inoculum, water-retaining agent and fermentation washing cow dung
It is formed according to mass ratio 1:0.1~0.2:20；The solid drought resisting microbial inoculum is by activation humic acid and includes bacillus subtilis
The microbial fermentation solution of (Bacillus subtilis) GE3 is mixed by solid-liquid ratio 5:1~2W/V；The bacillus subtilis
Bacterium (Bacillus subtilis) GE3 deposit number is CGMCC No.11966；Preferably, the system of the fermentation washing cow dung
Make method are as follows: washing cow dung raw material is subjected to decomposed, pile heap height > 1m by the way of the fermentation of pile, is wider than 3m, length >
20m carries out turning after heap temperature raising, and fermentation time is 7~15d, it is decomposed air-dry after crushed 1~2mm sieve to get；
Preferably, drought-resisting biological organic fertilizer contains total living bacteria count >=0.3 × 108cfu/g。
The present invention is retained drought-resistant organic fertilizer, with the solid drought resisting microbial inoculum containing bacillus subtilis GE3 and fermentation washing ox
Excrement cooperates a small amount of water-retaining agent as main component, and each ingredient collective effect is remarkably improved the fertility of soil, effectively promotees
Growth and drought-resistant ability into forest, fertility is lasting, and long-time service can improve soil environment.
The 5th aspect of the present invention provides the preparation method of water conservation drought-resisting biological organic fertilizer described above, by solid drought resisting
Microbial inoculum, water-retaining agent and fermentation washing cow dung obtain according to being sufficiently mixed in the ratio investment mixing machine of mass ratio 1:0.1~0.2:20
To mixture, mixture is crushed；The material crushed is put into Squeezinggranulator and is pelletized, cylindrical particle is put into dryer
In, drying temperature control at 50 DEG C hereinafter, be dried to water content≤15%, then it is cooling after screening packaging to get.
The present invention be retained the preparation method of drought-resisting biological organic fertilizer drying temperature controlled at 50 DEG C hereinafter, moisture content≤
15%, advantageously reduce high temperature, the dry influence to microbial activities such as bacillus subtilis GE3, biological organic of the present invention
Fertile preparation method is simple, is easy to large-scale production.
The 6th aspect of the present invention provides application of the water conservation drought-resisting biological organic fertilizer described above in afforestation.It is husky
Desertization, drought environment are the main bugbears that afforestation faces；And present invention water conservation drought-resisting biological organic fertilizer can be greatly facilitated woods
Growth of the wood in drought environment improves the drought-resistant stress ability of forest, improves survival rate of the forest in drought environment.
It is that the present invention obtains the utility model has the advantages that
The present invention provides the bacillus subtilis GE3 of one plant of great drought-resistant ability, the bacterial strain in drought environment not
But have stronger colonization ability, and can also significantly improve forest in drought environment at rate, promote the growth of forest.
Absorption carrier of the activation humic acid that the method for the present invention is prepared as bacillus subtilis GE3, can not only have
Effect absorption thallus, is conducive to being uniformly mixed for thallus and other ingredients, but also can protect the vigor of bacillus subtilis GE3,
It prevents from being granulated the damage to thallus vigor, and can advantageously promote bacillus subtilis GE3 in the soil environment of arid
Colonize and breed.
The present invention is retained drought-resisting biological organic fertilizer with the solid drought resisting microbial inoculum containing bacillus subtilis GE3, fermentation washing
Cow dung is main component, adds a small amount of water-retaining agent, and each ingredient interaction can be greatly facilitated life of the forest in drought environment
It is long, the drought-resistant stress ability of forest is improved, survival rate of the forest in drought environment is improved.Biological organic fertilizer of the present invention is not
But it is effectively utilized cow dung resource, improves the utilization rate of agricultural wastes；And it is significant to afforest for forest.
Detailed description of the invention
The Figure of description for constituting a part of the invention is used to provide further understanding of the present invention, and of the invention shows
Examples and descriptions thereof are used to explain the present invention for meaning property, does not constitute improper limitations of the present invention.
Fig. 1 bacillus subtilis (Bacillus subtilis) GE3 colony characteristics figure.
The strain classification that the present invention screens is named as bacillus subtilis (Bacillussubtilis) GE3, in
On January 7th, 2016 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and deposit number is
CGMCC No.11966, preservation address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica, postal
It compiles: 100101.
Specific embodiment
It is noted that described further below be all exemplary, it is intended to provide further instruction to the present invention.Unless another
It indicates, all technical and scientific terms used herein has usual with general technical staff of the technical field of the invention
The identical meanings of understanding.
It should be noted that term used herein above is merely to describe specific embodiment, and be not intended to restricted root
According to exemplary embodiments of the present invention.As used herein, unless the context clearly indicates otherwise, otherwise singular
Also it is intended to include plural form, additionally, it should be understood that, when in the present specification using term "comprising" and/or " packet
Include " when, indicate existing characteristics, step, operation and/or their combination.
In order to enable those skilled in the art can clearly understand technical solution of the present invention, below with reference to tool
The embodiment of the body technical solution that the present invention will be described in detail.
1 bacillus subtilis of embodiment (Bacillus subtilis) GE3 identification
Disclosure bacillus subtilis is that the applicant screens gained.
The colony characteristics of the plant rhizosphere growth-promoting bacterium are as follows: GE3 bacterial strain bacterium colony on LB culture medium is rounded irregular,
Bacterium colony size is about 5mm for 24 hours for culture, and dry tack free tarnish edge is irregular, and bacterium colony is in brown color and intermediate color is deeper, no
It is transparent, there is gemma.
The 16S rRNA gene order of GE3 and number Genbank are subjected to BLAST according to the sequence in library and compare analysis, as a result
Show: GE3 and Bacillus subtilis are in a smallest branch, the 16SrRNA gene of the nearest DZ1 of evolutionary distance together
The similitude of sequence and Bacillus subtilis (AJ276351) are up to 98.92%.Comprehensive physiological and biochemical property and colonial morphology
Feature is accredited as bacillus subtilis (Bacillus subtilis).
Bacillus subtilis (Bacillus subtilis) GE3 is inoculated in skimmed milk power culture medium with sterilizing toothpick to put down
On plate, after 37 DEG C of culture 2d, transparent circle can produce.It through measurement and calculates, transparent loop diameter is 9.73mm, and colony diameter is
17.08mm, transparent circle and bacterium colony ratio are up to 1.76.
The specific 16S rDNA sequence of bacillus subtilis GE3 is as follows:
gcggctggctcctaaaaggttacctcaccgacttcgggtgttacaaactctcgtggtgtgacgggcgg
tgtgtacaaggcccgggaacgtattcaccgcggcatgctgatccgcgattactagcgattccagcttcacgcagtc
gagttgcagactgcgatccgaactgagaacagatttgtgggattggcttaacctcgcggtttcgctgccctttgtt
ctgtccattgtagcacgtgtgtagcccaggtcataaggggcatgatgatttgacgtcatccccaccttcctccggt
ttgtcaccggcagtcaccttagagtgcccaactgaatgctggcaactaagatcaagggttgcgctcgttgcgggac
ttaacccaacatctcacgacacgagctgacgacaaccatgcaccacctgtcactctgcccccgaaggggacgtcct
atctctaggattgtcagaggatgtcaagacctggtaaggttcttcgcgttgcttcgaattaaaccacatgctccac
cgcttgtgcgggcccccgtcaattcctttgagtttcagtcttgcgaccgtactccccaggcggagtgcttaatgcg
ttagctgcagcactaaggggcggaaaccccctaacacttagcactcatcgtttacgg cgtggactaccagggtat
ctaatcctgttcgctccccacgctttcgctcctcagcgtcagttacagaccagagagtcgccttcgccactggtgt
tcctccacatctctacgcatttcaccgctacacgtggaattccactctcctcttctgcactcaagttccccagttt
ccaatgaccctccccggttgagccgggggctttcacatcagacttaagaaaccgcctgcgagccctttacgcccaa
taattccggacaacgcttgccacctacgtattaccgcggctgctggcacgtagttagccgtggctttctggttagg
taccgtcaaggtaccgccctattcgaacggtacttgttcttccctaacaacagagctttacgatccgaaaaccttc
atcactcacgcggcgttgctccgtcagactttcgtccattgcggaagattccctactgctgcctcccgtaggagtc
tgggccgtgtctcagtcccagtgtggccgatcaccctctcaggtcggctacgcatcgttgccttggtgagccgtta
cctcaccaactagctaatgcgccgcgggtccatctgtaagtggtagccgaagccaccttttatgtttgaaccatgc
ggttcaaacaaccatccggtattagccccggtttcccggagttatcccagtcttacaggcaggttacccacgtgtt
actcacccgtccgccgctaacatcagggagcaagctcccatctgtccgctcgactgca。
Bacillus subtilis (Bacillus subtilis) GE3 physiological and biochemical property is as shown in table 1 below.
1 GE3 bacterial strain physiological and biochemical property of table
It is as shown in table 2 below that bacillus subtilis (Bacillus subtilis) GE3 produces protease assay result.
2 GE3 strain protein enzyme activity determination of table
A kind of drought resisting solid fungicide of embodiment 2
The drought resisting solid fungicide the preparation method comprises the following steps:
(1) preparation method of humic acid is activated as the ratio by the nitric acid of Lignitic Humic Acid raw material and 20~25% according to 2:1
Example hybrid reaction 40min, centrifugation fall nitric acid solution, tap water are then added, and are centrifuged, operate repeatedly, until centrifugate pH > 5.5, dry
It is dry.
(2) preparation of bacillus subtilis (Bacillus subtilis) GE3 fermentation liquid:
1. test tube seed culture
Seed culture medium: beef extract 0.3%, peptone 1%, sodium chloride 0.5%, pH7.0~7.2.
Bacillus subtilis G3 is inoculated on culture medium, 30~35 DEG C of constant temperature incubations, obtains test tube species.
2. liquid fermentation and culture
Liquid fermentation medium formula are as follows: sucrose 3%, yeast extract 2%, K2HPO40.10%, MgSO4·7H2O
0.01%, CaCl20.01%, pH 6.5-7.0, surplus are water.
Bacillus subtilis G3 test tube seed is inoculated into fluid nutrient medium respectively, inoculum concentration is respectively fluid nutrient medium
The 8% of volume obtains bacillus subtilis G3 fermentation liquid in 30~35 DEG C of shaking table cultures after culture.
(3) the activation humic acid being prepared and bacillus subtilis (Bacillus subtilis) GE3 fermentation liquid are pressed
Solid-liquid ratio 5:1kg/L is mixed to obtain the final product.
Drought resisting solid fungicide living bacteria count >=1 × 109cfu/g。
Bacillus subtilis (Bacillus subtilis) GE3 fermentation liquid is inoculated into respectively according to the ratio of 5:1kg/L
In activation humic acid, Lignitic Humic Acid raw material, turf and chicken manure after sterilizing.It is air-dried under natural conditions, difference is measured after 30d to be had
Living bacteria count in machine material, concrete outcome are as shown in table 3 below.
The measurement of 3 bacillus subtilis GE3 viable count in different carriers of table
Under the conditions of natural air drying it can be seen from above-mentioned table 3, the bacillus subtilis GE3 activated in humic acid is effectively living
Bacterium number highest is suitable for the growth of GE3.
A kind of water conservation drought-resisting biological organic fertilizer of embodiment 3
The water conservation drought-resisting biological organic fertilizer is by solid drought resisting microbial inoculum, water-retaining agent and fermentation washing cow dung according to mass ratio 1:
0.1:20 composition；The solid drought resisting microbial inoculum is by activation humic acid and includes bacillus subtilis (Bacillus subtilis)
The microbial fermentation solution of GE3 is mixed by solid-liquid ratio 5:1kg/L.
The drought-resisting biological organic fertilizer contains total living bacteria count >=0.3 × 108cfu/g。
The preparation method of the water conservation drought-resisting biological organic fertilizer:
(1) production method that cow dung is washed in fermentation is stated are as follows: washing cow dung raw material is subjected to corruption by the way of pile fermentation
Ripe, pile heap height > 1m is wider than 3m, and length > 20m carries out turning after heap temperature raising, and fermentation time is 7~15d, rotten
It is ripe air-dry after crushed 1~2mm sieve to get.
(2) preparation of solid drought resisting microbial inoculum: preparation method is the same as embodiment 2.
(3) solid drought resisting microbial inoculum, water-retaining agent and fermentation washing cow dung are put into according to the ratio of mass ratio 1:0.1:20 mixed
It is sufficiently mixed in conjunction machine, obtains mixture, mixture is crushed；The material crushed is put into Squeezinggranulator and is pelletized, it will
Cylindrical particle is put into dryer, and drying temperature is controlled at 50 DEG C hereinafter, being dried to water content≤15%, then sieve packet after cooling down
Dress to get.
Influence of the 1 bacillus subtilis GE3 bacterium of test example to tree growth and drought resistance
By bacillus subtilis GE3, bacillus cereus Z77, A Shi bacillus BPR078, bacillus fusiformis L13,
Bacillus subtilis DZ1 be respectively connected to beef-protein medium (beef extract 0.3%, peptone 1%, sodium chloride 0.5%,
Agar 2%, pH 7.0-7.2 are mass percent above), in 37 DEG C, shaken cultivation 2d under the conditions of 180rpm.Fermentation liquid
6000r min-1Lower centrifugation 5min, after sterile saline rinse thallus 3 times, sterile saline adjusting bacteria suspension (5 ×
108Cfu/ml Inoculant) is made.Potting basin high 25cm, wide 30cm, every basin fill soil 12kg, select almost the same elder
Container seedling colonizes.After 30d, configured 5 kinds different bacterium solution 10ml are taken, 100ml is diluted to, uniformly pours respectively in elder
Around seedling root system, while being arranged and pouring the test of equivalent sterile saline.Elder does not rewater after sprinkling profoundly water, and arid is after 30 days
Measure elder upgrowth situation, blade relative conductivity and leaf r elative water content.Using be not inoculated with the potting of any microbial inoculum as
Blank control (CK).
Bacillus cereus (Bacillus cereus) Z77, to screen gained the applicant's early period, in 2012
On December 31, in is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number CGMCC
No.7070 is documented in Chinese patent " one plant of bacillus cereus and its application in raising plant drought ability " (CN
In 103667132A).
A Shi bacillus BPR078 is to screen gained the applicant's early period, is preserved in China on December 31st, 2012
Microbiological Culture Collection administration committee common micro-organisms center, deposit number are CGMCC No.11971.
The bacillus fusiformis L13, to screen gained, deposit number CGMCCNo.7068, the bacterium the application early period
Strain and its application have obtained national inventing patent authorization, patent authorization number: 2013100166040.
Bacillus subtilis DZ1 deposit number is CGMCC No.5318, and on October 14th, 2011 is in Chinese microorganism strain
The common micro-organisms center preservation of preservation administration committee.Bacillus subtilis DZ1 is the applicant's early period from winter jujube rhizosphere soil
In the plant growth-promoting rhizobacteria that filters out, which has been authorized (Patent No.: 201110376966.1)
Test result is as shown in table 4 below.
Influence of the 4 GE3 bacterium solution of table to tree growth and drought resistance
By above-mentioned table 4 it is found that bacillus subtilis GE3 of the present invention is to the drought resistance for promoting tree growth, improving forest
It is significantly higher than existing plant growth-promoting rhizobacteria, and is significantly higher than the bacillus subtilis DZ1 bacterial strain of same strain.
Influence of 2 drought-resisting biological organic fertilizer of test example to afforestation effect
Drought-resisting biological organic fertilizer afforestation experiment is arranged in the Beishan Mountain Niu Wangquan of the Laiwu City Laicheng district town the Miao Shan mountain village Bei Miao.It makes
Woods nursery stock selects gold and silver flower seedling.Apply drought-resisting biological organic fertilizer (experimental group, embodiment 3 are retained drought-resisting biological organic fertilizer), chicken manure
Organic fertilizer does not apply any fertilizer as blank (blank group) as control (control group).It is after batch has dug plant pit, drought resisting is raw
Organic fertilizer and backfill are mixed according to 1:1 ratio, are backfilling into afforestation plant pit.Afforestation same day watering is primary, and the later period is not pouring
Water.Investigation afforestation current year investigation survival rate, and survival rate and dry weight are investigated in next year, the results are shown in Table 5.
Growth, survival rate and the storage rate of 5 different disposal arbor-vitae of table
It can be seen from above-mentioned table 5 compared with control group and blank, the dry weight that drought-resisting biological organic fertilizer handles honeysuckle is aobvious
It writes and increases by 17.38% and 20.68%, and the afforestation survival rate and retention rate of honeysuckle has different degrees of raising.Therefore, In
In drought environment, drought-resisting biological organic fertilizer has positive effect in terms of improving the surrival rate of afforestation.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
SEQUENCE LISTING
<110>Shandong Forest Science Academy
<120>a kind of biological organic fertilizer and preparation method thereof with water conservation drought resisting function
<130>
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1417
<212> DNA
<213>artificial sequence
<400> 1
gcggctggct cctaaaaggt tacctcaccg acttcgggtg ttacaaactc tcgtggtgtg 60
acgggcggtg tgtacaaggc ccgggaacgt attcaccgcg gcatgctgat ccgcgattac 120
tagcgattcc agcttcacgc agtcgagttg cagactgcga tccgaactga gaacagattt 180
gtgggattgg cttaacctcg cggtttcgct gccctttgtt ctgtccattg tagcacgtgt 240
gtagcccagg tcataagggg catgatgatt tgacgtcatc cccaccttcc tccggtttgt 300
caccggcagt caccttagag tgcccaactg aatgctggca actaagatca agggttgcgc 360
tcgttgcggg acttaaccca acatctcacg acacgagctg acgacaacca tgcaccacct 420
gtcactctgc ccccgaaggg gacgtcctat ctctaggatt gtcagaggat gtcaagacct 480
ggtaaggttc ttcgcgttgc ttcgaattaa accacatgct ccaccgcttg tgcgggcccc 540
cgtcaattcc tttgagtttc agtcttgcga ccgtactccc caggcggagt gcttaatgcg 600
ttagctgcag cactaagggg cggaaacccc ctaacactta gcactcatcg tttacggcgt 660
ggactaccag ggtatctaat cctgttcgct ccccacgctt tcgctcctca gcgtcagtta 720
cagaccagag agtcgccttc gccactggtg ttcctccaca tctctacgca tttcaccgct 780
acacgtggaa ttccactctc ctcttctgca ctcaagttcc ccagtttcca atgaccctcc 840
ccggttgagc cgggggcttt cacatcagac ttaagaaacc gcctgcgagc cctttacgcc 900
caataattcc ggacaacgct tgccacctac gtattaccgc ggctgctggc acgtagttag 960
ccgtggcttt ctggttaggt accgtcaagg taccgcccta ttcgaacggt acttgttctt 1020
ccctaacaac agagctttac gatccgaaaa ccttcatcac tcacgcggcg ttgctccgtc 1080
agactttcgt ccattgcgga agattcccta ctgctgcctc ccgtaggagt ctgggccgtg 1140
tctcagtccc agtgtggccg atcaccctct caggtcggct acgcatcgtt gccttggtga 1200
gccgttacct caccaactag ctaatgcgcc gcgggtccat ctgtaagtgg tagccgaagc 1260
caccttttat gtttgaacca tgcggttcaa acaaccatcc ggtattagcc ccggtttccc 1320
ggagttatcc cagtcttaca ggcaggttac ccacgtgtta ctcacccgtc cgccgctaac 1380
atcagggagc aagctcccat ctgtccgctc gactgca 1417
Claims (8)
1. a kind of bacillus subtilis (Bacillus subtilis) GE3, which is characterized in that in preservation on January 7 in 2016
In China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCC No.11966.
2. bacillus subtilis described in claim 1 is improving the application in plant drought ability, which is characterized in that the plant
For elder, honeysuckle.
3. a kind of drought resisting microbial inoculum, which is characterized in that as activation humic acid and include the micro- of bacillus subtilis described in claim 1
Bio-fermented liquid mixes.
4. a kind of drought resisting microbial inoculum according to claim 3, which is characterized in that by activation humic acid and include bacillus subtilis
The microbial fermentation solution of bacterium (Bacillus subtilis) GE3 is mixed by solid-liquid ratio 5:1~2W/V, and the W/V is kg/
L；Bacillus subtilis (Bacillus subtilis) the GE3 deposit number is CGMCC No.11966；The drought resisting microbial inoculum
Living bacteria count >=1 × 109cfu/g。
5. a kind of drought resisting microbial inoculum according to claim 3 or 4, which is characterized in that the preparation method of the activation humic acid
For ratio 20~40min of hybrid reaction by the nitric acid of Lignitic Humic Acid raw material and 20~25% according to 2:1, it is molten that nitric acid is fallen in centrifugation
Then tap water is added in liquid, centrifugation operates repeatedly, until centrifugate pH > 5.5, drying.
6. a kind of water conservation drought-resisting biological organic fertilizer, which is characterized in that by drought resisting microbial inoculum, water-retaining agent and fermentation washing cow dung according to matter
Amount is formed than 1:0.1~0.2:20；
Microorganism of the drought resisting microbial inoculum by activation humic acid and comprising bacillus subtilis (Bacillus subtilis) GE3
Fermentation liquid is mixed by solid-liquid ratio 5:1~2W/V, and the W/V is kg/L；Bacillus subtilis (Bacillus subtilis)
GE3, deposit number are CGMCC No.11966；The water conservation drought-resisting biological organic fertilizer containing total living bacteria count >=0.3 ×
108cfu/g；
The preparation method comprises the following steps:
By drought resisting microbial inoculum, water-retaining agent and fermentation washing cow dung according in the ratio investment mixing machine of mass ratio 1:0.1~0.2:20
It is sufficiently mixed, obtains mixture, mixture is crushed；
The material crushed is put into Squeezinggranulator and is pelletized, cylindrical particle is put into dryer, drying temperature control exists
50 DEG C hereinafter, be dried to water content≤15%, then it is cooling after screening packaging to get.
7. a kind of water conservation drought-resisting biological organic fertilizer according to claim 6, which is characterized in that the fermentation washing cow dung
Production method are as follows: washing cow dung raw material is subjected to decomposed, pile heap height > 1m by the way of the fermentation of pile, wide > 3m, length >
20m carries out turning after heap temperature raising, and fermentation time is 7~15d, it is decomposed air-dry after crushed 1~2mm sieve to get.
8. a kind of application of the water conservation drought-resisting biological organic fertilizer described in claim 6 or 7 in plant afforestation, the plant are gold
Honeysuckle flower.
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| CN114686398B (en) * | 2022-03-17 | 2022-10-11 | 山东省林业科学研究院 | Biological water-retaining material and preparation method and application thereof |
| CN115286455A (en) * | 2022-08-24 | 2022-11-04 | 海南省百药林中医药健康产业有限公司 | Organic fertilizer capable of remarkably improving drought resistance of cymbidium sinense rice and preparation method thereof |
| CN115417418B (en) * | 2022-10-09 | 2023-07-25 | 甘肃中科启泰生物工程有限公司 | Microorganism modified attapulgite ore powder and preparation method and application thereof |
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