CN109679839A - A kind of placenta myeloid-lymphoid stem cell isolates and purifies device - Google Patents
A kind of placenta myeloid-lymphoid stem cell isolates and purifies device Download PDFInfo
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- CN109679839A CN109679839A CN201811588295.3A CN201811588295A CN109679839A CN 109679839 A CN109679839 A CN 109679839A CN 201811588295 A CN201811588295 A CN 201811588295A CN 109679839 A CN109679839 A CN 109679839A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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Abstract
The invention discloses a kind of placenta myeloid-lymphoid stem cells to isolate and purify device, including top cover labyrinth, temperature and humidity controller, door body, cabinet, concussion centrifuge tube, bottom sealing plate, purification beaker, fixed rack, firm banking, limit fixed block, data connection socket, filter screen and connection page.The beneficial effects of the present invention are: temperature and humidity controller guarantees the internal suitability for isolating and purifying environment, ensure the activity of placenta myeloid-lymphoid stem cell, concussion centrifuge tube prevents centrifugation from solution being caused to spill, ensure going on smoothly for centrifugal work, and it facilitates installation and removal, purification beaker facilitates separation and purification process that can complete in a kind of equipment, and it conveniently fetches and fixes, prevent spilling for purification liquid, the fluency and stability that fixed rack ensures to operate, and facilitate the progress of purification work, it is identical that filter screen respectively forms cell size shape, and it is fixed on above bottom sealing plate by way of slot, improve working efficiency, simplify operating procedure, facilitate its installation, disassembly and replacement.
Description
Technical field
The present invention relates to one kind to isolate and purify device, and specially a kind of placenta myeloid-lymphoid stem cell isolates and purifies device, belongs to
Biological cell separating and purifying technology field.
Background technique
A cell death, while thering are a considerable amount of neoblasts to generate, metabolism is formed, these neoblasts are all from dry
Cell, stem cell be a group have self-replacation and multi-lineage potential cell, they can constantly self-renewing, and
Transformation differentiation becomes one or more cell or tissue organs with specific function under specified conditions, in recent years, with research
Go deep into, have been able to extract in the tissue such as bleeding of the umbilicus, umbilical cord, placenta and cultivate various stem cells, wherein placenta has, and " stem cell is precious
Library " title, it is valuable life resource that stem cell population, which is equivalent to the stem cell backup an of human body, in placenta.
Currently, placenta myeloid-lymphoid stem cell isolates and purifies device still there are some problems, one, existing placenta all can be done
Cell isolates and purifies device and typically carries out step by step in isolation and purification, and thus more troublesome period will appear some dry
Immunity problem, secondly, existing placenta myeloid-lymphoid stem cell isolate and purify device be not much control in internal environment this respect,
Cell activity can be had some impact in this way, thirdly, existing placenta myeloid-lymphoid stem cell isolate and purify device after separation,
It often all needs to filter, dispels internal impurity, step is relatively complicated.
Summary of the invention
The object of the invention is that providing a kind of placenta myeloid-lymphoid stem cell to solve the above-mentioned problems isolates and purifies dress
It sets.
The present invention through the following technical solutions to achieve the above objectives: a kind of placenta myeloid-lymphoid stem cell isolates and purifies device,
Including top cover labyrinth, temperature and humidity controller, door body, cabinet, concussion centrifuge tube, bottom sealing plate, purification beaker, fixed rack, fixation
Pedestal, limit fixed block, data connection socket, filter screen and connection page；The top cover labyrinth is the platform of isosceles trapezoid in side view
Shape structure, and it is located above cabinet, the temperature and humidity controller is placed on cabinet wall, and the door body is connected by connection page
It connects on cabinet, the cabinet collectively forms the closed cavity of a cuboid with door body, and the delivery pipe upper end is connected to spinning
The lower section of fiber tube, lower end connect sliding slot, and the cylindrical rotation of concussion centrifuge tube is connected to below top cover labyrinth, the bottom
Sealing plate is installed below earthquake centrifuge tube, and the purification beaker is placed on fixed rack, and the fixed rack is located in the box body
Side bottom, the firm banking are fixed below cabinet by way of welding, and the limit fixed block disposes in a rectangular parallelepiped shape
In purification beaker two sides, the data connection socket is placed on firm banking, under the filter screen installation earthquake centrifuge tube
Side, and be located above bottom sealing plate, the connection page is symmetrically connected between cabinet and door body.
Preferably, in order to guarantee the internal suitability for isolating and purifying environment, it is ensured that the activity of placenta myeloid-lymphoid stem cell, it is described
The mode that temperature and humidity controller is screwed is connected on cabinet wall, and it electrically connect with outer controller.
Preferably, in order to prevent centrifugation cause solution to spill, it is ensured that centrifugal work is gone on smoothly, and its facilitate installation with
Disassembly, concussion centrifuge tube being threadedly secured in the screw hole of top cover labyrinth by the upper end, and its lower end is equipped with and can be rotated
Bottom sealing plate.
Preferably, separation and purification process can be completed in a kind of equipment for convenience, and conveniently fetch and fix, and prevent
Purification liquid is spilt, and the purification beaker bottom is equipped with the sliding block coincideing with fixed rack track, and end opening is located at shake thereon
It swings immediately below centrifuge tube.
Preferably, in order to ensure the fluency of operation and stability, and facilitate the progress of purification work, the fixed rack
With cabinet in integrated connection, and its sliding rail face is smooth.
Preferably, in order to improve working efficiency, simplify operating procedure, facilitate its installation, disassembly and replacement, the filter screen
Each composition cell size shape is identical, and it is fixed on above bottom sealing plate by way of slot.
The beneficial effects of the present invention are: the placenta myeloid-lymphoid stem cell isolates and purifies device design rationally, temperature and humidity controller
The mode being screwed is connected on cabinet wall, and it electrically connect with outer controller, guarantees internal isolate and purify
The suitability of environment, it is ensured that the activity of placenta myeloid-lymphoid stem cell, concussion centrifuge tube are threadedly secured in sealing by the upper end
In the screw hole of lid, and its lower end be equipped with rotatable bottom sealing plate, prevent centrifugation cause solution to spill, it is ensured that centrifugal work it is smooth
It carries out, and it facilitates installation and removal, purification beaker bottom is equipped with the sliding block coincideing with fixed rack track, and end opening thereon
Immediately below concussion centrifuge tube, facilitate separation and purification process that can complete in a kind of equipment, and conveniently fetch and fix, prevents
Only purify liquid to spill, fixed rack is with cabinet in integrated connection, and its sliding rail face is smooth, it is ensured that the fluency of operation with surely
It is qualitative, and facilitate the progress of purification work, it is identical that filter screen respectively forms cell size shape, and it is fixed by way of slot
Above bottom sealing plate, working efficiency is improved, simplifies operating procedure, facilitates its installation, disassembly and replacement.
Detailed description of the invention
Fig. 1 is schematic structural view of the invention；
Fig. 2 is schematic diagram at structure of the invention filter screen.
In figure: 1, top cover labyrinth, 2, temperature and humidity controller, 3, door body, 4, cabinet, 5, concussion centrifuge tube, 6, bottom sealing plate, 7,
Beaker is purified, 8, fixed rack, 9, firm banking, 10, limit fixed block, 11, data connection socket, 12, filter screen and 13 connect
Connect page.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Please refer to Fig. 1 ~ 2, a kind of placenta myeloid-lymphoid stem cell isolates and purifies device, including top cover labyrinth 1, temperature and humidity controller
2, door body 3, cabinet 4, concussion centrifuge tube 5, bottom sealing plate 6, purification beaker 7, fixed rack 8, firm banking 9, limit fixed block 10,
Data connection socket 11, filter screen 12 and connection page 13；The top cover labyrinth 1 is the mesa-shaped structure of isosceles trapezoid in side view, and
It is located at 4 top of cabinet, and the temperature and humidity controller 2 is placed on 4 inner wall of cabinet, and the door body 3 is connected by connection page 13
On cabinet 4, the cabinet 4 collectively forms the closed cavity of a cuboid with door body 3, and 5 upper end of delivery pipe is connected to
The lower section of spin duct 3, lower end connect sliding slot 12, and the cylindrical rotation of concussion centrifuge tube 5 is connected under top cover labyrinth 1
Side, the bottom sealing plate 6 install 5 lower section of earthquake centrifuge tube, and the purification beaker 7 is placed on fixed rack 8, described fixed sliding
Rail 8 is located at 4 inside bottom of cabinet, and the firm banking 9 is fixed on 4 lower section of cabinet by way of welding, and the limit is fixed
Block 10 is placed in purification 7 two sides of beaker in a rectangular parallelepiped shape, and the data connection socket 11 is placed on firm banking 9, the mistake
Strainer 12 installs 5 lower section of earthquake centrifuge tube, and is located at 6 top of bottom sealing plate, and the connection page 13 is symmetrically connected to cabinet 4 and door
Between body 3.
The mode that the temperature and humidity controller 2 is screwed is connected on 4 inner wall of cabinet, and it is in outer controller
It is electrically connected, guarantees the internal suitability for isolating and purifying environment, it is ensured that the activity of placenta myeloid-lymphoid stem cell, the concussion centrifuge tube
5 being threadedly secured in the screw hole of top cover labyrinth 1 by the upper end, and its lower end is equipped with rotatable bottom sealing plate 6, prevents from being centrifuged
Solution is caused to spill, it is ensured that centrifugal work is gone on smoothly, and it facilitates installation and removal, and 7 bottom of purification beaker is equipped with
The sliding block coincideing with 8 track of fixed rack, and end opening is located at immediately below concussion centrifuge tube 5 thereon, facilitates separation and purifying behaviour
Work can be completed in a kind of equipment, and conveniently fetch and fix, and prevent spilling for purification liquid, the fixed rack 8 is in cabinet 4
Integration connection, and its sliding rail face is smooth, it is ensured that the fluency and stability of operation, and facilitate the progress of purification work, it is described
Respectively composition cell size shape is identical for filter screen 12, and it is fixed on 6 top of bottom sealing plate by way of slot, improves work effect
Rate simplifies operating procedure, facilitates its installation, disassembly and replacement.
Working principle: when isolating and purifying device using the placenta myeloid-lymphoid stem cell, first ensure that each component can be just first
Then placenta myeloid-lymphoid stem cell is placed in concussion centrifuge tube 5, suitable temperature and humidity is arranged, guarantee stem cell by often work
Activity, then will purification liquid pour into purification beaker 7 in, it is fixed, starter filter screen 12 respectively composition cell size
Shape is identical, and it is fixed on 6 top of bottom sealing plate by way of slot, improves working efficiency, simplifies operating procedure, facilitate it
Installation, disassembly and replacement.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case where without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Benefit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent elements of the claims
Variation is included within the present invention.Any reference signs in the claims should not be construed as limiting the involved claims.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Claims (6)
1. a kind of placenta myeloid-lymphoid stem cell isolates and purifies device, it is characterised in that: including top cover labyrinth (1), temperature and humidity controller
(2), door body (3), cabinet (4), concussion centrifuge tube (5), bottom sealing plate (6), purification beaker (7), fixed rack (8), firm banking
(9), fixed block (10), data connection socket (11), filter screen (12) and connection page (13) are limited；The top cover labyrinth (1) is in
Side view is the mesa-shaped structure of isosceles trapezoid, and it is located above cabinet (4), and the temperature and humidity controller (2) is placed in cabinet (4)
On inner wall, the door body (3) is connected on cabinet (4) by connection page (13), and the cabinet (4) collectively forms with door body (3)
The closed cavity of one cuboid, delivery pipe (5) upper end are connected to the lower section of spin duct (3), and lower end connects sliding slot
(12), the cylindrical rotation of the concussion centrifuge tube (5) is connected to below top cover labyrinth (1), and the bottom sealing plate (6) is mounted on shake
It swings below centrifuge tube (5), the purification beaker (7) is placed on fixed rack (8), and the fixed rack (8) is located at cabinet (4)
Inside bottom, the firm banking (9) are fixed below cabinet (4) by way of welding, and the limit fixed block (10) is in
Rectangular-shape is placed in purification beaker (7) two sides, and the data connection socket (11) is placed on firm banking (9), the mistake
Strainer (12) is installed below earthquake centrifuge tube (5), and is located above bottom sealing plate (6), and the connection page (13) is symmetrically connected to
Between cabinet (4) and door body (3).
2. a kind of placenta myeloid-lymphoid stem cell according to claim 1 isolates and purifies device, it is characterised in that: the temperature and humidity
The mode that controller (2) is screwed is connected on cabinet (4) inner wall, and it electrically connect with outer controller.
3. a kind of placenta myeloid-lymphoid stem cell according to claim 1 isolates and purifies device, it is characterised in that: it is described shake from
Heart pipe (5) is threadedly secured in the screw hole of top cover labyrinth (1) by the upper end, and its lower end is equipped with rotatable bottom sealing plate
(6).
4. a kind of placenta myeloid-lymphoid stem cell according to claim 1 isolates and purifies device, it is characterised in that: the purification is burnt
Cup (7) bottom is equipped with the sliding block coincideing with fixed rack (8) track, and end opening is located at immediately below concussion centrifuge tube (5) thereon.
5. a kind of placenta myeloid-lymphoid stem cell according to claim 1 isolates and purifies device, it is characterised in that: described fixed sliding
Rail (8) is connected with cabinet (4) in integrated, and its sliding rail face is smooth.
6. a kind of placenta myeloid-lymphoid stem cell according to claim 1 isolates and purifies device, it is characterised in that: the filter screen
(12) respectively composition cell size shape is identical, and it is fixed on above bottom sealing plate (6) by way of slot.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811588295.3A CN109679839A (en) | 2018-12-25 | 2018-12-25 | A kind of placenta myeloid-lymphoid stem cell isolates and purifies device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811588295.3A CN109679839A (en) | 2018-12-25 | 2018-12-25 | A kind of placenta myeloid-lymphoid stem cell isolates and purifies device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109679839A true CN109679839A (en) | 2019-04-26 |
Family
ID=66189225
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811588295.3A Withdrawn CN109679839A (en) | 2018-12-25 | 2018-12-25 | A kind of placenta myeloid-lymphoid stem cell isolates and purifies device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109679839A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110157602A (en) * | 2019-05-27 | 2019-08-23 | 遵义医学院附属医院 | A kind of the sorting filter device and its application method of tumour cell group |
-
2018
- 2018-12-25 CN CN201811588295.3A patent/CN109679839A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110157602A (en) * | 2019-05-27 | 2019-08-23 | 遵义医学院附属医院 | A kind of the sorting filter device and its application method of tumour cell group |
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| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20190426 |
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| WW01 | Invention patent application withdrawn after publication |