CN109674870A - A kind of antibacterial growth promotion Chinese medicine composition and preparation method thereof and detection method - Google Patents
A kind of antibacterial growth promotion Chinese medicine composition and preparation method thereof and detection method Download PDFInfo
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/742—Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/46—Eucommiaceae (Eucommia family), e.g. hardy rubber tree
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/539—Scutellaria (skullcap)
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
- A61K36/718—Coptis (goldthread)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/734—Crataegus (hawthorn)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/752—Citrus, e.g. lime, orange or lemon
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
- A61K9/1605—Excipients; Inactive ingredients
- A61K9/1629—Organic macromolecular compounds
- A61K9/1652—Polysaccharides, e.g. alginate, cellulose derivatives; Cyclodextrin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/20—Pills, tablets, discs, rods
- A61K9/28—Dragees; Coated pills or tablets, e.g. with film or compression coating
- A61K9/2806—Coating materials
- A61K9/2833—Organic macromolecular compounds
- A61K9/284—Organic macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyvinyl pyrrolidone
- A61K9/2846—Poly(meth)acrylates
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/20—Pills, tablets, discs, rods
- A61K9/28—Dragees; Coated pills or tablets, e.g. with film or compression coating
- A61K9/2806—Coating materials
- A61K9/2833—Organic macromolecular compounds
- A61K9/286—Polysaccharides, e.g. gums; Cyclodextrin
- A61K9/2866—Cellulose; Cellulose derivatives, e.g. hydroxypropyl methylcellulose
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/12—Antidiarrhoeals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
Abstract
A kind of antibacterial growth promotion Chinese medicine composition, which is characterized in that be made of the raw material of following parts by weight: 2-10 parts of bacillus subtilis；5-35 parts of Radix Pulsatillae extract；5-40 parts of Baical Skullcap root P.E；5-35 parts of coptis extract；30-70 parts of eucommia leaf extract；20-50 parts of haw thorn extract；5-35 parts of dried orange peel extracts；5-35 parts of cortex fraxini extract；5-30 parts of licorice.The Chinese medicine composition is pharmaceutically acceptable dosage form: tablet, pill, powder, capsule, granule, liquid agent.The present invention also provides the preparation methods and detection method of a kind of antibacterial growth promotion Chinese medicine composition.The present invention can be reduced the addition of antibiotic or part substitute antibiotics and chemical synthesis class drug in feed, animal product medicament residue is reduced or avoided, it can effectively solve sucking pig, weanling pig, growing and fattening pigs, fowl diarrhea problem, it is remarkably decreased pig fowl diarrhea rate and the death rate, it is mainly used for pig, fowl, as mixed fodder additive, it can directly make an addition to feed or farm's spice uses.
Description
Technical field
The present invention relates to feed addictives, especially with a kind of Chinese medicinal compound extract and probiotic composition and its preparation
Method is related with detection method.
Background technique
FAQs: hypoimmunity is cultivated, illness is easy；The easy diarrhea of young animal, survival rate need to improve；Feedstuff-meat ratio
Height, feeding cost are high；It is influenced vulnerable to environment, temperature etc., stress reaction is strong.As cultivation density increases, disease prevention and control
Difficulty processed increases, and causes abuse of antibiotics and Pharmacological Level of Zinc preparation to abuse, meat pollutes (antibiotic and heavy metal), environmental pollution
(animal excrements heavy metal pollution soil) event takes place frequently.It is asked for food safety hazards present in cultivation and environmental pollution
Topic, national policy policy are put into effect in succession, are directed to the addition of limitation antibiotic and high zinc, and future gradually moves back antibiotic and high zinc
Out.The requirement of " green, safe and healthy, nonreactive " is constantly suggested and advocates, using good Chinese medicine class and microbiology class environmental protection
Type additive substitute antibiotics and high zinc become the developing direction of industry transition.
Currently on the market Common Chinese Herbs extract as additive dosage form be pulvis, in practical applications, often to
Family causes dust to perplex, and causes no small influence, pulvis mobility and dispersion to unclassified stores, product, producers and product
Property it is poor, cause mixing uneven, influence finished product quality, after common pulvis is searched for food by animal, be directly entered in the stomach of animal,
It is directly contacted with gastric acid, many active materials will receive destruction, and clinical application effect is undesirable.
Summary of the invention
For above-mentioned prior art defect, the present invention proposes a kind of animal health type additive substitute antibiotics, reach with
The identical antibacterial growth-promoting effect of antibiotic is specifically to provide a kind of Chinese medicinal compound extract and probiotic composition.The present invention
Another purpose is to provide the preparation method and detection method of a kind of the composition.
The present invention uses following technology:
A kind of antibacterial growth promotion Chinese medicine composition, which is characterized in that be made of the raw material of following parts by weight:
2-10 parts of bacillus subtilis；
5-35 parts of Radix Pulsatillae extract；
5-40 parts of Baical Skullcap root P.E；
5-35 parts of coptis extract；
30-70 parts of eucommia leaf extract；
20-50 parts of haw thorn extract；
5-35 parts of dried orange peel extracts；
5-35 parts of cortex fraxini extract；
5-30 parts of licorice.
Further, the content requirement of the bacillus subtilis are as follows: be not less than 1.0 × 109CFU/g；
The content requirement of the Radix Pulsatillae extract are as follows: B containing pulchinenoside4Not less than 10%；
The content requirement of the Baical Skullcap root P.E are as follows: be not less than 80% containing scutelloside；
The content requirement of the coptis extract are as follows: hydrochloric jamaicin is not less than 50%；
The content requirement of the eucommia leaf extract are as follows: be not less than 1% containing chlorogenic acid；
The content requirement of the haw thorn extract are as follows: be not less than 15% in terms of citric acid containing organic acid；
The content requirement of the dried orange peel extracts are as follows: be not less than 50% containing aurantiamarin；
The content requirement of the cortex fraxini extract are as follows: be not less than 10% containing aesculin and aesculetin summation；
The content requirement of the licorice are as follows: be not less than 8% containing glycyrrhizic acid.
Further, the Chinese medicine composition be pharmaceutically acceptable dosage form: tablet, pill, powder, capsule, granule,
Liquid agent.
The present invention also provides a kind of preparation methods of antibacterial growth promotion Chinese medicine composition, it is characterised in that:
Filler, adhesive, glidant are taken, and the antibacterial growth promotion Chinese medicine group as described in any one of claim 1 ~ 3
Object is closed, 60 ~ 100 mesh screens are crossed, it is spare；
8.2 ~ 34 parts by weight of Chinese medicine composition, 60 ~ 90.3 parts by weight of filler are weighed, are uniformly mixed, 1 ~ 4.5 parts by weight are added
Adhesive is added the glidant of 0.5 ~ 1.5 parts by weight after total mix, Chinese medicine group is made through softwood processed, pelleting, drying, whole grain
Close the granule of object；
Wherein:
The filler is one of starch, dextrin, sucrose, lactose, microcrystalline cellulose, croscarmellose sodium or more
Kind；
Described adhesive is one of water, povidone, hydroxypropyl methylcellulose, hydroxypropylcellulose；
The glidant is one of superfine silica gel powder, sodium stearyl fumarate, magnesium stearate, talcum powder.
Further, the Adding Way of adhesive are as follows: adhesive is added in the mixture of Chinese medicine composition and filler,
It is uniformly mixed, the viscosity that water causes each raw material is added；Or after adhesive is dissolved in water, it is then added to Chinese medicine composition and filling
Viscosity is generated in the mixture of agent.
Another preparation method of antibacterial growth promotion Chinese medicine composition provided by the invention, it is characterised in that:
Filler, adhesive, glidant are taken, and the antibacterial growth promotion Chinese medicine group as described in any one of claim 1 ~ 3
Object is closed, 60 ~ 100 mesh screens are crossed, it is spare；
0.5 ~ 1.5 parts by weight of 5 ~ 15 parts by weight of enteric-coating material, 3.5 ~ 6.5 parts by weight of antitackiness agent and plasticizer are taken, adds water, matches
The enteric coating liquid of solid content 5% ~ 25% is made, it is spare；
8.2 ~ 34 parts by weight of Chinese medicine composition, 38.5 ~ 81.8 parts by weight of filler are weighed, are uniformly mixed；
It is added in adhesive to the mixture of Chinese medicine composition and filler of 1 ~ 4.5 parts by weight and prepares softwood and using extrusion
Round as a ball pellet processing machine prepares pellet, or the mixture of Chinese medicine composition and filler is added in centrifugation pellet processing machine and spray into 1 ~
The adhesive of 4.5 parts by weight prepares pellet；
It is dry；
By the dry pellet obtained, it is placed in coating equipment in sugar production line, the enteric coating liquid for spraying into preparation is coated, and makes its coating weight gain
Reach 5% ~ 40%, the pill of Chinese medicine composition is made.
Wherein,
Filler is one of starch, dextrin, sucrose, lactose, microcrystalline cellulose, croscarmellose sodium or a variety of；
Adhesive is one of water, povidone, hydroxypropyl methylcellulose, hydroxypropylcellulose；
Glidant is one of superfine silica gel powder, sodium stearyl fumarate, magnesium stearate, talcum powder；
Enteric-coating material is methacrylic acid copolymer A type, methacrylic acid copolymer Type B, methacrylic acid copolymer C
One of type, hypromellose phthalate are a variety of；
Antitackiness agent is one of talcum powder, glycerin monostearate；
Plasticizer is one of propylene glycol, triethyl citrate, Macrogol 6000.
The present invention also provides a kind of this detection methods of antibacterial growth promotion Chinese medicine composition, which is characterized in that the detection
Method detects Chinese medicine composition pulchinenoside B using high performance liquid chromatography4, scutelloside, chlorogenic acid, Berberine hydrochloride contain
Amount:
(1) pulchinenoside B4, measured according to high performance liquid chromatography (" Chinese veterinary pharmacopoeia " 2015 editions second annex 47)
Chromatographic condition and system suitability test, using octadecylsilane chemically bonded silica as filler；It is with methanol-water 64:36
Mobile phase；Detection wavelength is 201nm；Theoretical cam curve presses pulchinenoside B4Peak, which calculates, should be not less than 3000；
The preparation of reference substance solution takes pulchinenoside B4Appropriate reference substance, it is accurately weighed, add methanol that every 1ml is made containing 0.1mg
Solution to get；
The preparation of test solution takes this product 0.2g, accurately weighed, sets in stuffed conical flask, adds methanol 10ml, close plug, ultrasound
Processing, power 150W, frequency 40KHZ, 25 minutes are let cool, and are filtered, and filtrate is set in 250ml measuring bottle, wash appearance with a small amount of mobile phase
Device and residue, washing lotion are incorporated in same measuring bottle, add mobile phase to scale, shake up to get；
Measuring method, it is accurate respectively to draw reference substance solution and each 20 μ l of test solution, inject liquid chromatograph, measurement to get.
(2) scutelloside is measured according to high performance liquid chromatography (" Chinese veterinary pharmacopoeia " 2015 editions second annex 47):
Chromatographic condition and system suitability test, using octadecylsilane chemically bonded silica as filler；It is molten with -0.2% phosphoric acid of methanol
Liquid 47:53 is mobile phase；Detection wavelength is 280nm；Theoretical cam curve is calculated by scutelloside peak should be not less than 1000；
The preparation of reference substance solution takes scutelloside reference substance 10mg, accurately weighed, sets in 100ml measuring bottle, adds methanol and be diluted to
Scale shakes up；Precision measure 5ml, set in 10ml measuring bottle, be diluted with water to scale, shake up to get；
The preparation of test solution takes this product 0.5g, accurately weighed, sets in 250ml measuring bottle, adds methanol 200ml ultrasound 10 minutes,
It lets cool, adds methanol to scale, shake up, filter, take subsequent filtrate；Precision measures 5ml, sets in 10ml measuring bottle, is diluted with water to scale,
Shake up, take subsequent filtrate to get；
Measuring method, it is accurate respectively to draw reference substance solution and each 10 μ l of test solution, inject liquid chromatograph, measurement to get.
(3) chlorogenic acid is measured according to high performance liquid chromatography (" Chinese veterinary pharmacopoeia " 2015 editions second annex 47):
Chromatographic condition and system suitability test, using octadecylsilane chemically bonded silica as filler；With -0.4% phosphoric acid of acetonitrile
Solution 13:87 is mobile phase；Detection wavelength is 327nm；Theoretical cam curve is calculated by chlorogenic acid peak should be not less than 2000；
The preparation of reference substance solution takes chlorogenic acid reference substance 5mg, accurately weighed, sets in 25ml brown measuring bottle, adds 50% methanol
20ml ultrasound 20 minutes, lets cool, with 50% methanol dilution to scale, shake up to get；
The preparation of test solution takes this product 2.5g, accurately weighed, sets in 25ml brown measuring bottle, adds 50% methanol 20ml ultrasound 20
Minute, let cool, with 50% methanol dilution to scale, shake up, filter, take subsequent filtrate to get；
Measuring method, it is accurate respectively to draw reference substance solution and each 20 μ l of test solution, inject liquid chromatograph, measurement to get.
(4) Berberine hydrochloride is measured according to high performance liquid chromatography (" Chinese veterinary pharmacopoeia " 2015 editions second annex 47):
Chromatographic condition and system suitability test, using octadecylsilane chemically bonded silica as filler；With acetonitrile -0.05mol/L
Add lauryl sodium sulfate 0.4g in potassium dihydrogen phosphate 50:50, every 100ml, then with phosphorus acid for adjusting pH value for 4.0, for stream
Dynamic phase；Detection wavelength is 345nm；Theoretical cam curve is calculated by Berberine hydrochloride peak should be not less than 5000；
The preparation of reference substance solution takes Berberine hydrochloride reference substance appropriate, accurately weighed, adds methanol that every ml is made containing 90.5 μ g's
Solution to get；
The preparation of test solution takes this product about 0.2g, accurately weighed, is added in 150ml measuring bottle, and methanol-hydrochloric acid is added in precision
100:1 50ml, closed, weighing；It is ultrasonically treated 30min, is let cool；Weighing, the weight of less loss is supplied with methanol；It shakes up, filters,
Take subsequent filtrate 1ml, be added in 50ml measuring bottle, methanol is added to scale, shake up to get；
Measuring method, it is accurate respectively to draw reference substance solution and each 20 μ l of test solution, inject liquid chromatograph, measurement to get.
The invention has the advantages that:
1, Chinese medicine composition of the invention can effectively solve sucking pig, weanling pig, growing and fattening pigs, fowl diarrhea problem, make pig fowl diarrhea
Rate and the death rate are remarkably decreased；Daily ingestion amount can be effectively improved, accelerates pig speed of weight increment obviously, and feed can be significantly improved
Conversion ratio, reduce feedstuff-meat ratio, be obviously shortened pig marketing time, can be used for treating animal diarrhea have loose bowels, alimentary infection, on
Respiratory tract infection promotes digestion, increases feed intake, strengthen immunity, improves anti-stress ability；
2, Chinese medicine composition of the invention reduces antibiotic or part substitute antibiotics and chemical synthesis class drug in feed
Addition, is reduced or avoided animal product medicament residue, strengthen immunity is prevented and cured diseases；Efficient antidiarrheal reduces young animal diarrhea
Incidence improves survival rate and healthy vigor；It improves food conversion ratio, reduces feedstuff-meat ratio, promote growth of animal, raising increases day by day
Weight；Animal anti-stress ability is improved, animal health status is significantly improved or improve；Auxiliary improves clinical application effect, such as Yi Wei
Rhzomorph, Tetracyclines, macrolides；Endocrine system is influenced, animal carcasses composition is improved, improves animal products quality, it is main
It is used for pig, fowl, as mixed fodder additive, feed can be directly made an addition to or farm's spice uses；
3, Chinese medicine composition of the invention, additive amount is few, raw material sources are stable, and stringent incoming inspection, flavour of a drug are reasonably combined, and with
Probiotics is used cooperatively, and not by mould contamination, easily stores, heavy metal free residual, multi-efficiency, effect stability；
4, pulchinenoside B4, scutelloside, Berberine hydrochloride, the component contents such as chlorogenic acid it is clear, establish science, accurately
Detection method；
5, traditional Chinese medicine particle produced by the present invention, mobility of particle is good, and appearance looks elegant is uniformly dispersed, and moisture is no more than 8%,
It avoids agglomerating, go mouldy；Chinese medicine composition enteric-coated micro-pill produced by the present invention, pellet good fluidity, appearance looks elegant are uniformly dispersed,
Dosage is accurate, and moisture is no more than 3%, avoids agglomerating, and goes mouldy；Release in enteron aisle, release completely, by being coated covering smell, improve
Animal feed intake, bioavilability are high.
Detailed description of the invention
Fig. 1 is the HPLC spectrogram of reference substance scutelloside.
Fig. 2 is the HPLC spectrogram of reference substance Berberine hydrochloride.
Fig. 3 is the HPLC spectrogram of reference substance chlorogenic acid.
Fig. 4 is the HPLC spectrogram of 1 content determination of Baicalin of embodiment.
Fig. 5 is the HPLC spectrogram of 1 berberine hydrochloride content of embodiment.
Fig. 6 is the HPLC spectrogram of 1 determination of chlorogenic acid of embodiment.
Specific embodiment
In order to keep the purpose, technical solution and specific implementation method of the application apparent, in conjunction with specific embodiments to this
Application is further elaborated.
Embodiment 1
According to following raw material composition Chinese medicine composition: 2 parts of bacillus subtilis；5 parts of Radix Pulsatillae extract；Radix scutellariae extracts
5 parts of object；5 parts of coptis extract；30 parts of eucommia leaf extract；20 parts of haw thorn extract；5 parts of dried orange peel extracts；Cortex fraxini extract 5
Part；5 parts of licorice.
Wherein, the content requirement of the bacillus subtilis are as follows: be not less than 1.0 × 109CFU/g.The Chinese bulbul is extracted
The content requirement of object are as follows: B containing pulchinenoside4Not less than 10%；The content requirement of the Baical Skullcap root P.E are as follows: not containing scutelloside
Lower than 80%；The content requirement of the coptis extract are as follows: hydrochloric jamaicin is not less than 50%；The eucommia leaf extract contains
Amount requires are as follows: is not less than 1% containing chlorogenic acid；The content requirement of the haw thorn extract are as follows: be not less than in terms of citric acid containing organic acid
15%；The content requirement of the dried orange peel extracts are as follows: be not less than 50% containing aurantiamarin；The content requirement of the cortex fraxini extract are as follows:
It is not less than 10% containing aesculin and aesculetin summation；The content requirement of the licorice are as follows: be not less than containing glycyrrhizic acid
8%。
Take filler-starch, adhesive-povidone, glidant-superfine silica gel powder, and the antibacterial growth promotion Chinese medicine of configuration
Composition crosses 60 mesh screens, spare；
8.2 parts by weight of Chinese medicine composition, 60 parts by weight of filler are weighed, are uniformly mixed, the adhesive of 1 parts by weight are added, specifically
It is to be added to adhesive in the mixture of Chinese medicine composition and filler, is uniformly mixed, water is added and causes the viscous of each raw material
Property；Through softwood processed, pelleting, drying, whole grain, the glidant of 0.5 parts by weight is added after total mix, of Chinese medicine composition is made
Granula.
Embodiment 2
According to following raw material composition Chinese medicine composition: 10 parts of bacillus subtilis；35 parts of Radix Pulsatillae extract；Radix scutellariae mentions
Take 40 parts of object；35 parts of coptis extract；70 parts of eucommia leaf extract；50 parts of haw thorn extract；35 parts of dried orange peel extracts；The bark of ash mentions
Take 35 parts of object；30 parts of licorice.
Wherein, the content requirement of the bacillus subtilis are as follows: be not less than 1.0 × 109CFU/g.The Chinese bulbul is extracted
The content requirement of object are as follows: B containing pulchinenoside4Not less than 10%；The content requirement of the Baical Skullcap root P.E are as follows: not containing scutelloside
Lower than 80%；The content requirement of the coptis extract are as follows: hydrochloric jamaicin is not less than 50%；The eucommia leaf extract contains
Amount requires are as follows: is not less than 1% containing chlorogenic acid；The content requirement of the haw thorn extract are as follows: be not less than in terms of citric acid containing organic acid
15%；The content requirement of the dried orange peel extracts are as follows: be not less than 50% containing aurantiamarin；The content requirement of the cortex fraxini extract are as follows:
It is not less than 10% containing aesculin and aesculetin summation；The content requirement of the licorice are as follows: be not less than containing glycyrrhizic acid
8%。
Filler-croscarmellose sodium, adhesive-hydroxypropyl methylcellulose, glidant-magnesium stearate are taken, and
The antibacterial growth promotion Chinese medicine composition of configuration, sieves with 100 mesh sieve net, spare；
34 parts by weight of Chinese medicine composition, 90.3 parts by weight of filler are weighed, are uniformly mixed, the adhesive of 4.5 parts by weight, tool is added
Body is, after adhesive is dissolved in water, is then added in the mixture of Chinese medicine composition and filler and generates viscosity；Through softwood processed,
Pelleting, dry, whole grain are added the glidant of 1.5 parts by weight after total mix, the granule of Chinese medicine composition are made.
Embodiment 3
According to following raw material composition Chinese medicine composition: 6 parts of bacillus subtilis；15 parts of Radix Pulsatillae extract；Radix scutellariae mentions
Take 25 parts of object；25 parts of coptis extract；50 parts of eucommia leaf extract；35 parts of haw thorn extract；25 parts of dried orange peel extracts；The bark of ash mentions
Take 25 parts of object；15 parts of licorice.
Wherein, the content requirement of the bacillus subtilis are as follows: be not less than 1.0 × 109CFU/g.The Chinese bulbul is extracted
The content requirement of object are as follows: B containing pulchinenoside4Not less than 10%；The content requirement of the Baical Skullcap root P.E are as follows: not containing scutelloside
Lower than 80%；The content requirement of the coptis extract are as follows: hydrochloric jamaicin is not less than 50%；The eucommia leaf extract contains
Amount requires are as follows: is not less than 1% containing chlorogenic acid；The content requirement of the haw thorn extract are as follows: be not less than in terms of citric acid containing organic acid
15%；The content requirement of the dried orange peel extracts are as follows: be not less than 50% containing aurantiamarin；The content requirement of the cortex fraxini extract are as follows:
It is not less than 10% containing aesculin and aesculetin summation；The content requirement of the licorice are as follows: be not less than containing glycyrrhizic acid
8%。
Filler-lactose, adhesive-water, glidant-sodium stearyl fumarate are taken, and the antibacterial growth promotion configured
Chinese medicine composition crosses 80 mesh screens, spare；
25 parts by weight of Chinese medicine composition, 80 parts by weight of filler are weighed, are uniformly mixed, the adhesive of 2 parts by weight are added, specifically
It is after adhesive is dissolved in water, to be then added in the mixture of Chinese medicine composition and filler and generate viscosity；Through softwood processed, system
Particle, drying, whole grain are added the glidant of 1 parts by weight after total mix, the granule of Chinese medicine composition are made.
Embodiment 4
According to following raw material composition Chinese medicine composition: 5 parts of bacillus subtilis；10 parts of Radix Pulsatillae extract；Radix scutellariae mentions
Take 20 parts of object；20 parts of coptis extract；40 parts of eucommia leaf extract；30 parts of haw thorn extract；20 parts of dried orange peel extracts；The bark of ash mentions
Take 20 parts of object；20 parts of licorice.
Wherein, the content requirement of the bacillus subtilis are as follows: be not less than 1.0 × 109CFU/g.The Chinese bulbul is extracted
The content requirement of object are as follows: B containing pulchinenoside4Not less than 10%；The content requirement of the Baical Skullcap root P.E are as follows: not containing scutelloside
Lower than 80%；The content requirement of the coptis extract are as follows: hydrochloric jamaicin is not less than 50%；The eucommia leaf extract contains
Amount requires are as follows: is not less than 1% containing chlorogenic acid；The content requirement of the haw thorn extract are as follows: be not less than in terms of citric acid containing organic acid
15%；The content requirement of the dried orange peel extracts are as follows: be not less than 50% containing aurantiamarin；The content requirement of the cortex fraxini extract are as follows:
It is not less than 10% containing aesculin and aesculetin summation；The content requirement of the licorice are as follows: be not less than containing glycyrrhizic acid
8%。
Filler-microcrystalline cellulose, adhesive-hydroxypropylcellulose, glidant-talcum powder are taken, with the antibacterial growth-promoting of configuration
Long Chinese medicine composition crosses 70 mesh screens, spare；
Take enteric-coating material -5 parts by weight of methacrylic acid copolymer A type, 3.5 parts by weight of antitackiness agent-glycerin monostearate
With 0.5 parts by weight of plasticizer-propylene glycol, add water, is configured to the enteric coating liquid of solid content 5%, it is spare；
8.2 parts by weight of Chinese medicine composition, 38.5 parts by weight of filler are weighed, are uniformly mixed；
It is added in adhesive to the mixture of Chinese medicine composition and filler of 1 parts by weight and prepares softwood and use extrusion spheronization
Pellet processing machine prepares pellet；It is dry；The dry pellet obtained is placed in coating equipment in sugar production line, the enteric coating liquid for spraying into preparation carries out
Coating, makes its coating weight gain reach 5%, and the pill of Chinese medicine composition is made.
Embodiment 5
According to following raw material composition Chinese medicine composition: 6 parts of bacillus subtilis；15 parts of Radix Pulsatillae extract；Radix scutellariae mentions
Take 25 parts of object；25 parts of coptis extract；50 parts of eucommia leaf extract；35 parts of haw thorn extract；25 parts of dried orange peel extracts；The bark of ash mentions
Take 25 parts of object；15 parts of licorice.
Wherein, the content requirement of the bacillus subtilis are as follows: be not less than 1.0 × 109CFU/g.The Chinese bulbul is extracted
The content requirement of object are as follows: B containing pulchinenoside4Not less than 10%；The content requirement of the Baical Skullcap root P.E are as follows: not containing scutelloside
Lower than 80%；The content requirement of the coptis extract are as follows: hydrochloric jamaicin is not less than 50%；The eucommia leaf extract contains
Amount requires are as follows: is not less than 1% containing chlorogenic acid；The content requirement of the haw thorn extract are as follows: be not less than in terms of citric acid containing organic acid
15%；The content requirement of the dried orange peel extracts are as follows: be not less than 50% containing aurantiamarin；The content requirement of the cortex fraxini extract are as follows:
It is not less than 10% containing aesculin and aesculetin summation；The content requirement of the licorice are as follows: be not less than containing glycyrrhizic acid
8%。
Take filler-dextrin, adhesive-povidone, glidant-magnesium stearate, and the antibacterial growth promotion Chinese medicine of configuration
Composition crosses 90 mesh screens, spare；
Take enteric-coating material -15 parts by weight of methacrylic acid copolymer Type B, 6.5 parts by weight of antitackiness agent-talcum powder and plasticising
1.5 parts by weight of agent-triethyl citrate, add water, are configured to the enteric coating liquid of solid content 25%, spare；
34 parts by weight of Chinese medicine composition, 81.8 parts by weight of filler are weighed, are uniformly mixed；
The mixture of Chinese medicine composition and filler is added in centrifugation pellet processing machine to and is sprayed into the adhesive preparation of 4.5 parts by weight
Pellet；It is dry；By the dry pellet obtained, it is placed in coating equipment in sugar production line, the enteric coating liquid for spraying into preparation is coated, and makes its packet
Clothing weight gain reaches 40%, and the pill of Chinese medicine composition is made.
Embodiment 6
According to following raw material composition Chinese medicine composition: 5 parts of bacillus subtilis；30 parts of Radix Pulsatillae extract；Radix scutellariae mentions
Take 10 parts of object；10 parts of coptis extract；60 parts of eucommia leaf extract；28 parts of haw thorn extract；10 parts of dried orange peel extracts；The bark of ash mentions
Take 10 parts of object；10 parts of licorice.
Wherein, the content requirement of the bacillus subtilis are as follows: be not less than 1.0 × 109CFU/g.The Chinese bulbul is extracted
The content requirement of object are as follows: B containing pulchinenoside4Not less than 10%；The content requirement of the Baical Skullcap root P.E are as follows: not containing scutelloside
Lower than 80%；The content requirement of the coptis extract are as follows: hydrochloric jamaicin is not less than 50%；The eucommia leaf extract contains
Amount requires are as follows: is not less than 1% containing chlorogenic acid；The content requirement of the haw thorn extract are as follows: be not less than in terms of citric acid containing organic acid
15%；The content requirement of the dried orange peel extracts are as follows: be not less than 50% containing aurantiamarin；The content requirement of the cortex fraxini extract are as follows:
It is not less than 10% containing aesculin and aesculetin summation；The content requirement of the licorice are as follows: be not less than containing glycyrrhizic acid
8%。
Filler-starch, adhesive-hydroxypropylcellulose, glidant-sodium stearyl fumarate are taken, with the antibacterial configured
Growth promotion Chinese medicine composition sieves with 100 mesh sieve net, spare；
Take 10 parts by weight of enteric-coating material-hypromellose phthalate, 5 parts by weight of antitackiness agent-glycerin monostearate and
1 parts by weight of plasticizer-Macrogol 6000, add water, are configured to the enteric coating liquid of solid content 15%, spare；
20 parts by weight of Chinese medicine composition, 60 parts by weight of filler are weighed, are uniformly mixed；
It is added in adhesive to the mixture of Chinese medicine composition and filler of 3 parts by weight and prepares softwood and use extrusion spheronization
Pellet processing machine prepares pellet；It is dry；The dry pellet obtained is placed in coating equipment in sugar production line, the enteric coating liquid for spraying into preparation carries out
Coating, makes its coating weight gain reach 25%, and the pill of Chinese medicine composition is made.
Content detection
The sample obtained to embodiment 1 ~ 6 detects, and detects Chinese medicine composition pulchinenoside using high performance liquid chromatography
B4, scutelloside, chlorogenic acid, Berberine hydrochloride content:
(1) pulchinenoside B4, it is measured according to high performance liquid chromatography (" Chinese veterinary pharmacopoeia " 2015 editions second annex 47):
Chromatographic condition and system suitability test, using octadecylsilane chemically bonded silica as filler；It is with methanol-water 64:36
Mobile phase；Detection wavelength is 201nm；Theoretical cam curve presses pulchinenoside B4Peak, which calculates, should be not less than 3000；
The preparation of reference substance solution takes pulchinenoside B4Appropriate reference substance, it is accurately weighed, add methanol that every 1ml is made containing 0.1mg
Solution to get；
The preparation of test solution, the sample 0.2g that Example 1 ~ 6 obtains respectively is accurately weighed, sets in stuffed conical flask, adds
Methanol 10ml, close plug, ultrasonic treatment, power 150W, frequency 40KHZ, 25 minutes are let cool, and are filtered, and filtrate is set in 250ml measuring bottle,
Wash container and residue with a small amount of mobile phase, washing lotion is incorporated in same measuring bottle, adds mobile phase to scale, shake up to get；
Measuring method, the accurate each 20 μ l of test solution for drawing reference substance solution and embodiment 1 ~ 6, injects liquid chromatogram respectively
Instrument, measurement to get.
(2) scutelloside is measured according to high performance liquid chromatography (" Chinese veterinary pharmacopoeia " 2015 editions second annex 47):
Chromatographic condition and system suitability test, using octadecylsilane chemically bonded silica as filler；It is molten with -0.2% phosphoric acid of methanol
Liquid 47:53 is mobile phase；Detection wavelength is 280nm；Theoretical cam curve is calculated by scutelloside peak should be not less than 1000；
The preparation of reference substance solution takes scutelloside reference substance 10mg, accurately weighed, sets in 100ml measuring bottle, adds methanol and be diluted to
Scale shakes up；Precision measure 5ml, set in 10ml measuring bottle, be diluted with water to scale, shake up to get；
The preparation of test solution, the sample 0.5g that Example 1 ~ 6 obtains respectively is accurately weighed, sets in 250ml measuring bottle, adds
It methanol 200ml ultrasound 10 minutes, lets cool, adds methanol to scale, shake up, filter, take subsequent filtrate；Precision measures 5ml, sets 10ml amount
Bottle in, be diluted with water to scale, shake up, take subsequent filtrate to get；
Measuring method, the accurate each 10 μ l of test solution for drawing reference substance solution and embodiment 1 ~ 6, injects liquid chromatogram respectively
Instrument, measurement to get.
(3) chlorogenic acid is measured according to high performance liquid chromatography (" Chinese veterinary pharmacopoeia " 2015 editions second annex 47):
Chromatographic condition and system suitability test, using octadecylsilane chemically bonded silica as filler；With -0.4% phosphoric acid of acetonitrile
Solution 13:87 is mobile phase；Detection wavelength is 327nm；Theoretical cam curve is calculated by chlorogenic acid peak should be not less than 2000；
The preparation of reference substance solution takes chlorogenic acid reference substance 5mg, accurately weighed, sets in 25ml brown measuring bottle, adds 50% methanol
20ml ultrasound 20 minutes, lets cool, with 50% methanol dilution to scale, shake up to get；
The preparation of test solution, the sample 2.5g that Example 1 ~ 6 obtains respectively is accurately weighed, sets in 25ml brown measuring bottle,
Add 50% methanol 20ml ultrasound 20 minutes, let cool, with 50% methanol dilution to scale, shake up, filter, take subsequent filtrate to get；
Measuring method, the accurate each 20 μ l of test solution for drawing reference substance solution and embodiment 1 ~ 6, injects liquid chromatogram respectively
Instrument, measurement to get.
(4) Berberine hydrochloride is measured according to high performance liquid chromatography (" Chinese veterinary pharmacopoeia " 2015 editions second annex 47):
Chromatographic condition and system suitability test, using octadecylsilane chemically bonded silica as filler；With acetonitrile -0.05mol/L
Add lauryl sodium sulfate 0.4g in potassium dihydrogen phosphate 50:50, every 100ml, then with phosphorus acid for adjusting pH value for 4.0, for stream
Dynamic phase；Detection wavelength is 345nm；Theoretical cam curve is calculated by Berberine hydrochloride peak should be not less than 5000；
The preparation of reference substance solution takes Berberine hydrochloride reference substance appropriate, accurately weighed, adds methanol that every ml is made containing 90.5 μ g's
Solution to get；
The preparation of test solution, the sample about 0.2g that Example 1 ~ 6 obtains respectively, accurately weighed, addition 150ml measuring bottle
In, methanol-hydrochloric acid 100:150ml, closed, weighing is added in precision；It is ultrasonically treated 30min, is let cool；Weighing, is supplied with methanol and is subtracted
The weight of mistake；Shake up, filter, take subsequent filtrate 1ml, be added in 50ml measuring bottle, methanol is added to scale, shake up to get；
Measuring method, the accurate each 20 μ l of test solution for drawing reference substance solution and embodiment 1 ~ 6, injects liquid chromatogram respectively
Instrument, measurement to get.
Such as the HPLC spectrogram of Fig. 1 ~ 3 respectively scutelloside of reference substance, Berberine hydrochloride, chlorogenic acid.
If Fig. 4 ~ 6 are respectively the HPLC spectrogram of the scutelloside of embodiment 1, Berberine hydrochloride, chlorogenic acid.
Testing result:
Clinical test
Antibacterial growth promotion Chinese medicinal composition granules weanling pig applying clinical test prepared by embodiment 3
One, test objective:
Clinical effectiveness of the Chinese medicine composition grain product under normal additive capacity is investigated, dysentery is let out from growth promotion, prevention and treatment, improves
Immunity promotes feeding, improvement feedstuff-meat ratio etc. to be investigated.
Two, test material:
1, experimental animal
Kind: pig (tri-crossbreeding, PIC pig, black pig).
Age and gender:
Wean sucking pig: 30 ages in days or so, weight 8.5kg or so, male and female is half-and-half；
Health status: pig in good health is selected.
2, drug
Test medication: feed addictive prepared by embodiment 3: antibacterial growth promotion Chinese medicinal composition granules；
Compare medication: feeding antibiotic: 15% aureomycin.
3, experimental design
Selecting 30 ages in days to be averaged, three way cross weanling pig in good health 120 that weight is (8.5 ± 0.5) kg is (public
Mother respectively accounts for half), 2 groups are randomly divided into, every group of 6 repetitions, 10 pigs of every repetition, each repetition is 1 column.Feeding is used and is freely adopted
Food, daily to feed 2 times, free water.
1 group is test group, and the traditional Chinese medicine particle feed addictive of 1000g/t is added on basal diet；
2 groups are control group, add aureomycin according to the dosage of 500g/t on basal diet.
Test is divided into preliminary trial period (3d), positive to try phase (28d).
It is grouped constant, corresponding feed is fed according to each grouping after preliminary trial period, until off-test.
Three, test index
1, growth performance: the 4th day and the 31st day is tested in morning on an empty stomach by pig weight of only weighing, as initial stage weight and latter stage
Weight；Daily accurate recording is every to repeat suckling piglet feed intake, and each repetition suckling piglet average daily gain of calculating, averagely increase day by day quality
And feedstuff-meat ratio.
Calculation formula:
Average daily gain=(last weighing-first weighing)/test number of days
Average daily gain=total feed intake/test number of days
Feedstuff-meat ratio=average daily gain/average daily gain
2, stress reaction: according to diarrhea (head number, time, light and heavy degree), the dead and other morbidity numbers etc. recorded during test,
Calculate diarrhea rate, the death rate and the other disease incidence of each group.
Diarrhea rate/%=(diarrhea head number × Diarrhoea days)/(test pig's head number × test number of days) × 100%
Four, test result
1, growth performance
* there were significant differences compared with the control group, P < 0.05
2, stress reaction
Do not occur die of illness situation and other morbidities, therefore only counts diarrhea rate.
* there were significant differences compared with the control group, P < 0.05
Five, conclusion (of pressure testing)
Chinese medicine composition grain feed addictive substitute antibiotics are applied in combination in weanling pig daily ration, are remarkably improved disconnected
The growth performance and reduction diarrhea rate of milk piglet, it is identical as antibiotic to illustrate that Chinese medicine composition grain substitute antibiotics can reach
Antibacterial growth-promoting effect.
The indication of Chinese medicine composition of the invention:
1, for wean sodium selenite: resisting stress and its caused diarrhea are had loose bowels, and enhancing is immune；
2, for the piglet that has loose bowels: antibacterials adjuvant drug improves antibacterials curative effect, assists antidiarrheal；Increase feed intake, promotes
Digestion；
3, be directed to vaccine inoculation initial stage child care pig: antibiotic deactivates to be used in 7 days, improves immune, enhancing vaccine vigor；
4, growing and fattening pigs are directed to: increasing feed intake, promote digestion and growth；
5, to poultry: heat-clearing, removing toxic substances, stop dysentery；Feed intake is significantly improved, feedstuff-meat ratio is reduced, improves immunity；
6, as health-care good product, can be used for a long time, cope with it is all kinds of stress, enhancing is immune and synergist when can be used as vaccine application
And protective agent.
Chinese medicine composition of the invention, based on traditional Chinese medicine theory, using a variety of natural plant extracts as raw material,
Using modern crafts and referring to the research that Chinese medicine influences animal in the past 30 years, selected Broadspectrum antiphlogistic antimicrobial extract is main ingredient,
Preventing and treating gastrointestinal tract and infection of the upper respiratory tract extract is ministerial drug, is aided with strengthening the spleen and replenishing qi medicine；Using the 8 taste natural plants such as folium cortex eucommiae
Compound extract, rich in bioactive substances such as plant flavonoids, organic acid, polysaccharide and alkaloids.
Chinese medicine composition of the invention all has feature and advantage in terms of " color, shape, taste, effect ":
Color is close with feed in faint yellow；High temperature resistant, good fluidity are conducive to feed processing and produce；It is nontoxic, noresidue, agreeable to the taste
Property it is good, tool herbal medicine taste, it is environmentally protective；Obvious effect, multi-efficiency, effect stability.
Claims (10)
1. a kind of antibacterial growth promotion Chinese medicine composition, which is characterized in that be made of the raw material of following parts by weight:
2-10 parts of bacillus subtilis；
5-35 parts of Radix Pulsatillae extract；
5-40 parts of Baical Skullcap root P.E；
5-35 parts of coptis extract；
30-70 parts of eucommia leaf extract；
20-50 parts of haw thorn extract；
5-35 parts of dried orange peel extracts；
5-35 parts of cortex fraxini extract；
5-30 parts of licorice.
2. antibacterial growth promotion Chinese medicine composition according to claim 1, it is characterised in that: the bacillus subtilis contains
Amount requires are as follows: is not less than 1.0 × 109CFU/g。
3. antibacterial growth promotion Chinese medicine composition according to claim 1, it is characterised in that:
The content requirement of the Radix Pulsatillae extract are as follows: B containing pulchinenoside4Not less than 10%；
The content requirement of the Baical Skullcap root P.E are as follows: be not less than 80% containing scutelloside；
The content requirement of the coptis extract are as follows: hydrochloric jamaicin is not less than 50%；
The content requirement of the eucommia leaf extract are as follows: be not less than 1% containing chlorogenic acid；
The content requirement of the haw thorn extract are as follows: be not less than 15% in terms of citric acid containing organic acid；
The content requirement of the dried orange peel extracts are as follows: be not less than 50% containing aurantiamarin；
The content requirement of the cortex fraxini extract are as follows: be not less than 10% containing aesculin and aesculetin summation；
The content requirement of the licorice are as follows: be not less than 8% containing glycyrrhizic acid.
4. antibacterial growth promotion Chinese medicine composition described according to claim 1 ~ any one of 3, it is characterised in that: the Chinese medicine group
Conjunction object is pharmaceutically acceptable dosage form: tablet, pill, powder, capsule, granule, liquid agent.
5. a kind of preparation method of the antibacterial growth promotion Chinese medicine composition as described in any one of claim 1 ~ 3, feature
It is:
Filler, adhesive, glidant are taken, and the antibacterial growth promotion Chinese medicine group as described in any one of claim 1 ~ 3
Object is closed, 60 ~ 100 mesh screens are crossed, it is spare；
8.2 ~ 34 parts by weight of Chinese medicine composition, 60 ~ 90.3 parts by weight of filler are weighed, are uniformly mixed, 1 ~ 4.5 parts by weight are added
Adhesive is added the glidant of 0.5 ~ 1.5 parts by weight after total mix, Chinese medicine group is made through softwood processed, pelleting, drying, whole grain
Close the granule of object；
Wherein:
The filler is one of starch, dextrin, sucrose, lactose, microcrystalline cellulose, croscarmellose sodium or more
Kind；
Described adhesive is one of water, povidone, hydroxypropyl methylcellulose, hydroxypropylcellulose；
The glidant is one of superfine silica gel powder, sodium stearyl fumarate, magnesium stearate, talcum powder.
6. the preparation method of antibacterial growth promotion Chinese medicine composition according to claim 5, which is characterized in that adhesive adds
Enter method are as follows: adhesive is added in the mixture of Chinese medicine composition and filler, is uniformly mixed, water is added and causes each raw material
Viscosity；Or after adhesive is dissolved in water, it is then added in the mixture of Chinese medicine composition and filler and generates viscosity.
7. a kind of preparation method of the antibacterial growth promotion Chinese medicine composition as described in any one of claim 1 ~ 3, feature
It is:
Filler, adhesive, glidant are taken, and the antibacterial growth promotion Chinese medicine group as described in any one of claim 1 ~ 3
Object is closed, 60 ~ 100 mesh screens are crossed, it is spare；
0.5 ~ 1.5 parts by weight of 5 ~ 15 parts by weight of enteric-coating material, 3.5 ~ 6.5 parts by weight of antitackiness agent and plasticizer are taken, adds water, matches
The enteric coating liquid of solid content 5% ~ 25% is made, it is spare；
8.2 ~ 34 parts by weight of Chinese medicine composition, 38.5 ~ 81.8 parts by weight of filler are weighed, are uniformly mixed；
It is added in adhesive to the mixture of Chinese medicine composition and filler of 1 ~ 4.5 parts by weight and prepares softwood and using extrusion
Round as a ball pellet processing machine prepares pellet, or the mixture of Chinese medicine composition and filler is added in centrifugation pellet processing machine and spray into 1 ~
The adhesive of 4.5 parts by weight prepares pellet；
It is dry；
By the dry pellet obtained, it is placed in coating equipment in sugar production line, the enteric coating liquid for spraying into preparation is coated, and makes its coating weight gain
Reach 5% ~ 40%, the pill of Chinese medicine composition is made；
Wherein,
Filler is one of starch, dextrin, sucrose, lactose, microcrystalline cellulose, croscarmellose sodium or a variety of；
Adhesive is one of water, povidone, hydroxypropyl methylcellulose, hydroxypropylcellulose；
Glidant is one of superfine silica gel powder, sodium stearyl fumarate, magnesium stearate, talcum powder；
Enteric-coating material is methacrylic acid copolymer A type, methacrylic acid copolymer Type B, methacrylic acid copolymer C
One of type, hypromellose phthalate are a variety of；
Antitackiness agent is one of talcum powder, glycerin monostearate；
Plasticizer is one of propylene glycol, triethyl citrate, Macrogol 6000.
8. a kind of detection method of the antibacterial growth promotion Chinese medicine composition as described in any one of claim 1 ~ 3, feature
It is, the detection method detects Chinese medicine composition pulchinenoside B using high performance liquid chromatography4, scutelloside, chlorogenic acid,
The content of Berberine hydrochloride:
(1) pulchinenoside B4, it is measured according to high performance liquid chromatography (" Chinese veterinary pharmacopoeia " 2015 editions second annex 47):
Chromatographic condition and system suitability test, using octadecylsilane chemically bonded silica as filler；It is with methanol-water 64:36
Mobile phase；Detection wavelength is 201nm；Theoretical cam curve presses pulchinenoside B4Peak, which calculates, should be not less than 3000；
The preparation of reference substance solution takes pulchinenoside B4Appropriate reference substance, it is accurately weighed, add methanol that every 1ml is made containing 0.1mg
Solution to get；
The preparation of test solution takes this product 0.2g, accurately weighed, sets in stuffed conical flask, adds methanol 10ml, close plug, ultrasound
Processing, power 150W, frequency 40KHZ, 25 minutes are let cool, and are filtered, and filtrate is set in 250ml measuring bottle, wash appearance with a small amount of mobile phase
Device and residue, washing lotion are incorporated in same measuring bottle, add mobile phase to scale, shake up to get；
Measuring method, it is accurate respectively to draw reference substance solution and each 20 μ l of test solution, inject liquid chromatograph, measurement to get；
(2) scutelloside is measured according to high performance liquid chromatography (" Chinese veterinary pharmacopoeia " 2015 editions second annex 47):
Chromatographic condition and system suitability test, using octadecylsilane chemically bonded silica as filler；It is molten with -0.2% phosphoric acid of methanol
Liquid 47:53 is mobile phase；Detection wavelength is 280nm；Theoretical cam curve is calculated by scutelloside peak should be not less than 1000；
The preparation of reference substance solution takes scutelloside reference substance 10mg, accurately weighed, sets in 100ml measuring bottle, adds methanol and be diluted to
Scale shakes up；Precision measure 5ml, set in 10ml measuring bottle, be diluted with water to scale, shake up to get；
The preparation of test solution takes this product 0.5g, accurately weighed, sets in 250ml measuring bottle, adds methanol 200ml ultrasound 10 minutes,
It lets cool, adds methanol to scale, shake up, filter, take subsequent filtrate；Precision measures 5ml, sets in 10ml measuring bottle, is diluted with water to scale,
Shake up, take subsequent filtrate to get；
Measuring method, it is accurate respectively to draw reference substance solution and each 10 μ l of test solution, inject liquid chromatograph, measurement to get；
(3) chlorogenic acid is measured according to high performance liquid chromatography (" Chinese veterinary pharmacopoeia " 2015 editions second annex 47):
Chromatographic condition and system suitability test, using octadecylsilane chemically bonded silica as filler；With -0.4% phosphoric acid of acetonitrile
Solution 13:87 is mobile phase；Detection wavelength is 327nm；Theoretical cam curve is calculated by chlorogenic acid peak should be not less than 2000；
The preparation of reference substance solution takes chlorogenic acid reference substance 5mg, accurately weighed, sets in 25ml brown measuring bottle, adds 50% methanol
20ml ultrasound 20 minutes, lets cool, with 50% methanol dilution to scale, shake up to get；
The preparation of test solution takes this product 2.5g, accurately weighed, sets in 25ml brown measuring bottle, adds 50% methanol 20ml ultrasound 20
Minute, let cool, with 50% methanol dilution to scale, shake up, filter, take subsequent filtrate to get；
Measuring method, it is accurate respectively to draw reference substance solution and each 20 μ l of test solution, inject liquid chromatograph, measurement to get；
(4) Berberine hydrochloride is measured according to high performance liquid chromatography (" Chinese veterinary pharmacopoeia " 2015 editions second annex 47):
Chromatographic condition and system suitability test, using octadecylsilane chemically bonded silica as filler；With acetonitrile -0.05mol/L
Add lauryl sodium sulfate 0.4g in potassium dihydrogen phosphate 50:50, every 100ml, then with phosphorus acid for adjusting pH value for 4.0, for stream
Dynamic phase；Detection wavelength is 345nm；Theoretical cam curve is calculated by Berberine hydrochloride peak should be not less than 5000；
The preparation of reference substance solution takes Berberine hydrochloride reference substance appropriate, accurately weighed, adds methanol that every ml is made containing 90.5 μ g's
Solution to get；
The preparation of test solution takes this product 0.2g, accurately weighed, is added in 150ml measuring bottle, and methanol-hydrochloric acid is added in precision
100:1 50ml, closed, weighing；It is ultrasonically treated 30min, is let cool；Weighing, the weight of less loss is supplied with methanol；It shakes up, filters,
Take subsequent filtrate 1ml, be added in 50ml measuring bottle, methanol is added to scale, shake up to get；
Measuring method, it is accurate respectively to draw reference substance solution and each 20 μ l of test solution, inject liquid chromatograph, measurement to get.
9. antibacterial growth promotion Chinese medicine composition described according to claim 1 ~ any one of 3 is drawn in preparation treatment animal diarrhea
Dilute, alimentary infection, the infection of the upper respiratory tract promote digestion, increase feed intake, strengthen immunity, the medicine for improving anti-stress ability
Purposes in object.
10. the feed addictive containing the antibacterial growth promotion Chinese medicine composition as described in any one of claim 1 ~ 3.
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| CN112315934A (en) * | 2020-10-27 | 2021-02-05 | 广西馨海药业科技有限公司 | Preparation process of pulsatilla chinensis saponin B4 enteric-coated tablet |
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Application publication date: 20190426 |