CN1096652A - Bifidobacterium nutrient health beverage, food - Google Patents
Bifidobacterium nutrient health beverage, food Download PDFInfo
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- CN1096652A CN1096652A CN93107458A CN93107458A CN1096652A CN 1096652 A CN1096652 A CN 1096652A CN 93107458 A CN93107458 A CN 93107458A CN 93107458 A CN93107458 A CN 93107458A CN 1096652 A CN1096652 A CN 1096652A
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- powder
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- bifidobacterium
- zymotic fluid
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Abstract
The invention provides a kind of bifidobacterium nutrient health beverage, food.With the nutrition help material that is rich in carbon source such as radix polygoni multiflori powder etc., add other nutrient and form substrate after technical finesses such as acidifying, enzymolysis, cultivate the bifid bacterium and make zymotic fluid zymotic fluid and suitable additives or original fruit juice can be made multiple bifidobacterium nutrient health beverage.Or zymotic fluid made the Mixed Microbes powder, makes multiple bifidobacterium nutrient health food with auxiliary material through suitable technology again.The present invention has utilized the bifid bacterium that thereby the health-care effect of health and help material are developed the approach that bifid bacterium and help material are coordinated mutually, made full use of to the strengthening by means of tonics effect of human body.
Description
The present invention relates to a kind of health drink, food, particularly a kind of nutrient health beverage and food that is rich in bifid bacterium and help material.
At present, many active ingredients in many help materials that is rich in carbon source do not obtain rationally, make full use of, as the tuber of multiflower knotweed, the tuber of dwarf lilyturf, heart of a lotus seed benevolence etc., normally directly prescription decocts and takes or lixiviate, concentrate and make various cream, crystalline substance, oral liquid etc., its lixiviate or the component that decocts out only account for 2.5～7.5% of its total amount usually, and nutritional labelings such as a large amount of starch, protein nearly all are used as impurity and precipitate or filter removal.Even resemble directly processing and eating such as the tuber of multiflower knotweed, heart of a lotus seed benevolence, the absorption of its effective ingredient and the performance of nourishing health function also are sufficient inadequately.
The objective of the invention is to nutrition, the help material that is rich in carbon source serves as that the master forms substrate in order to cultivation bifid bacterium, and then makes health drink or the food that is rich in bifid bacterium and multiple help material.Owing to utilized the special health-care effect of bifid bacterium,, brought into play value and the nourishing and the health care of bifid bacterium and help material more fully so improved the result of use of former nutrition, help material greatly to human body.
Technical solution of the present invention is: to be rich in the nutrition help thing such as the radix polygoni multiflori powder of carbon source, lotus-seed-heart powder etc. and not only carbonaceous sources but also the beans etc. of being rich in protein be main, add an amount of other nutrient again and form substrate, by acidifying, culture medium is made in technical finesses such as enzymolysis, cultivate through test tube bifid bacterium bacterial classification, the cultivation of glass bottle and big jar of cultivation make is rich in bifid bacterium and multiple nutrients, the zymotic fluid of help material adds the proper flavour agent with this zymotic fluid, sweetener, suspending agent or original fruit juice, vegetable protein liquid etc. can be made into the bifidobacterium nutrient health beverage of multiple local flavor.Or with zymotic fluid by concentrate, the dry processing make the Mixed Microbes powder, and then with this bacterium powder and suitably auxiliary material be prepared into various bifidobacterium nutrient health foods (brilliant, chew beans, capsule etc.) with universal process.
1, bifid bacterium culture medium component prescription (weight ratio):
1.1 test tube culture medium
Glucose (lactose, maltose, sucrose, fructose) 1.0～3.0
Protein hydrolysate (skimmed milk power, animal blood hydrolysate, beef broth)
1.0～2.0
YE (doing) 0.0～0.5
K
2HPO
4（KH
2PO
4、CaHPO
4） 0.05～0.15
Nacl（Mgcl
2） 0.01～0.10
Water (mineral water, magnetized drinking water, medical stone immersion liquid) 50-65
1.2 glass bottle culture medium
Glucose (maltose, fructose, lactose, sucrose) 1.0～2.0
Skimmed milk power (protein hydrolysate, animal blood hydrolysate) 1.0～2.0
Ground rice (sweet potato flour, Tapioca starch, lotus root starch) 0.0～1.5
Bean powder (soybean, dregs of beans, pea, mung bean flour) 0.0～1.0
Hu Luobu powder 0.3～0.5
Marine alga (sea-tangle, laver, sea mustard powder) 0.0～0.35
YE (doing) 0.0～0.6
Malt flour (carbohydrase, protease) 0.0～0.35
Actinolite extract (doing) (CaHPO
4, Kcl, Nacl) 0.01～0.5
Radix polygoni multiflori powder (tuber of dwarf lilyturf, heart of a lotus seed benevolence, Chinese yam, lily, date, radix pseudostellariae etc.)
0.15～2.5
Water (mineral water, magnetized drinking water, medical stone immersion liquid) 50～65
1.3 big jar culture medium
Tapioca starch (ground rice, sweet potato flour, corn flour, lotus root starch) 0.6～2.0
Bean powder (soybean, dregs of beans, pea, mung bean flour) 0.5～1.2
Skimmed milk power (protein hydrolysate, animal blood hydrolysate) 1.0～1.5
Hu Luobu powder 0.3～0.5
Seawood meal (sea-tangle, laver, sea mustard powder) 0.0～0.35
Actinolite extract (CaHPO
4, Nacl, Kcl, Mgcl
2)
0.0～0.5
Malt flour (amylase, protease) 0.15～0.35
YE (B family vitamin, niacinamide, folic acid)
0.0～0.5
Radix polygoni multiflori powder (tuber of dwarf lilyturf, heart of a lotus seed benevolence, Chinese yam, lily, date, radix pseudostellariae etc.)
0.3～3.5
Water (mineral water, magnetized drinking water, medical stone immersion liquid) 50～65
2, technical process;
2.1 the bifid bacterium is cultivated
To after 25～30 minutes, reduce to 35～37 ℃ through 121 ℃ of sterilizations by in the 1.1 test tube culture medium injecting tubes of preparing of filling a prescription 2.1.1 test tube is cultivated, inoculation bifid bacterium bacterial classification leads to CO to the test tube bottom
2Gas 1～2 minute, airtight, cultivated 20～24 hours for 37 ℃, get the test tube nutrient solution.
2.1.2 cultivating, the glass bottle will behind the acidifying enzymolysis, inject suitable glass bottle by the glass bottle culture medium of prescription 1.2 preparations, reduce to 35～37 ℃ after 25～30 minutes through 121 ℃ of flowing steam sterilizations again, inoculate 3.5% well-grown above-mentioned test tube nutrient solution, to the logical CO that regulates flow in glass bottle bottom
2Gas or N
2Gas 1～3 minute, airtight, cultivated 24～30 hours for 37 ℃, get seed liquor.
2.1.3 big jar cultivate the starch-containing more material of will fill a prescription in 1.3 through acidifying (PH6～6.5) and temperature control (45 ℃～80 ℃) subsection enzymolysis, adding leftover materials, stir evenly, after the filtration, through radiation or high hot instantaneously sterilising, move in advance in the culture tank of hot pressing steam sterilization, or the culture medium after directly will filtering pumps into and uses flowing steam sterilization in the culture tank.Temperature adjustment to 35～37 ℃, the above-mentioned seed liquor of inoculation 3.0～3.5%, input capacity CO
2Gas or N
2Behind the gas, airtight, 37 ℃ of anaerobism cultivations got zymotic fluid in about 36 hours.
2.2 produce bifidobacterium nutrient health beverage
Above-mentioned zymotic fluid is added an amount of sweetener, flavouring agent, suspending agent and water can make bifidobacterium nutrient health beverage after homogeneous is handled with different characteristics.
Also above-mentioned zymotic fluid and various original fruit juice and vegetable protein liquid appropriate amount of auxiliary materials can be added again with proper ratio and technical process and multiple bifid bacterium fruit tea, the bifid bacterium nutrient health beverage of Compositional type such as really suckle can be made.
2.3 produce bifidobacterium nutrient health food (brilliant, chew beans, capsule etc.)
With above-mentioned zymotic fluid through temperature control, decompression, concentrate, make the Mixed Microbes powder that contains a large amount of bifid bacterium and multiple help material after the drying.With the combination of this Mixed Microbes powder and different auxiliary material and through suitably can make the bifidobacterium nutrient crystalline substance after the technical process processing, chew beans, bifidobacterium nutrient health food such as capsule.
The present invention has made full use of the bifid bacterium to health and long-lived closely related, growing and play the specific function of key and central role at aspects such as biological antagonist, immunity coordinations the infant, after being technical finesses such as main warp acidifying enzymolysis with the nutrition help material that is rich in carbon source, the starch that it is contained changes into monose or disaccharide, protein transduction is changed into peptone or amino acid etc. constitute substrate to cultivate propagation bifid bacterium.And then make bifidobacterium nutrient health beverage with good nourishing and health-care efficacy and the food that contains a large amount of bifid bacterium and multiple help material.
The new way that the present invention has opened up (as the tuber of multiflower knotweed, Chinese yam, heart of a lotus seed benevolence, the lotus root starch etc.) deep processing of help material and made full use of has the utmost point social benefit and economic benefit widely.
Embodiment: infant's bifid bacterium health drink
1, media components prescription (weight ratio)
1.1 test tube culture medium
Glucose 3.0 CaHPO
40.15
Protein hydrolysate powder 2.0 Nacl 0.01
Water 50 Kcl 0.01
1.2 glass bottle culture medium
Glucose 1.5 malt flours 0.15
Ground rice 0.6 skimmed milk power 1.5
Tapioca starch 0.5 CaHPO
40.15
Hu Luobu powder 0.3 Nacl 0.03
Date 0.15 Kcl 0.01
H
2O 50
1.3 big jar culture medium
Ground rice 0.6 skimmed milk power 2.0
Tapioca starch 1.2 sea-tangle powder 0.3
Hu Luobu powder 0.5 date 0.3
Malt flour 0.35 radix pseudostellariae powder 0.5
Actinolite extract 0.5 H
2O 50
2, technical process
2.1 test tube cultivate will culture medium injecting tube by 1.1 formulated in, behind pressure sterilizing, be cooled to 35～37 ℃, inoculation infant bifid bacterium bacterial classification feeds the CO of control flow velocity to the test tube bottom
2Gas or N
2Gas 1～1.5 minute seals the mouth of pipe with glassine paper, cultivates about 24 hours for 37 ℃, gets the test tube nutrient solution.
2.2 it is 6.0～6.5 that the cultivation of glass bottle will transfer to pH value with 10% lactic acid by the culture medium of 1.2 formulated, 45～80 ℃ of subsection enzymolysis of temperature control 3～4 hours, inject 1.5～3.0 liters of triangle glass bottles behind the cooling suction filtration, be cooled to 35～37 ℃ through 121 ℃ of hot steam sterilizers, inoculation 3.5～4.0% above-mentioned test tube nutrient solutions feed capacity CO to glass bottle bottom
2Gas or N
2Gas, airtight, cultivated 24 hours for 37 ℃, get seed liquor.
2.3 cultivate for big jar.Will be by the ground rice of 1.3 prescription meterings, malt flour, date, Tapioca starch, the Hu Luobu powder, the sea-tangle powder, transferring PH with 10% lactic acid behind radix pseudostellariae powder and the water mixing is 6.0～6.5, be warming up to 45～50 ℃, kept 2.5～3.0 hours, be warming up to 75～80 ℃ again, kept 1.5～2.0 hours, add leftover materials, be incubated 80 ℃, stirred 10～15 minutes, be cooled to about 50 ℃ with terylene filter cloth suction filtration, filtrate is pumped into after with radiation or moment high heat (high pressure) sterilization treatment in advance in 30 minutes culture tank of 121 ℃ of flowing steam sterilizations, temperature adjustment to 35～37 ℃, inoculation 3.0～3.5% above-mentioned seed liquor feed capacity CO
2Gas or N
2Behind the gas, airtight, cultivated about 36 hours for 37 ℃, get zymotic fluid.
2.4 preparation bifid bacteria beverage with above-mentioned zymotic fluid after the even matter of colloid mill, the fructose (or sucrose) of adding 6.0～8.0%, 0.06～0.08% stevioside, 0.5～1.2% xanthans, 0.1～0.3% emulsifying essence, 0.1～0.15 citric acid, after fully stirring, packing makes infant's bifid bacterium health drink.
Claims (3)
1, a kind of health drink that contains a large amount of bifid bacterium and multiple help material, food, it is characterized in that: with the nutrition help material that is rich in carbon source and not only carbonaceous sources but also the beans that contains nitrogenous source through acidifying, after the technical finesses such as enzymolysis, with bifid bacterium bacterial classification through test tube, glass bottle and big jar of cultivation preparation zymotic fluid, by zymotic fluid and different additive or original fruit juice, the allotment of vegetable protein liquid makes multiple bifidobacterium nutrient health beverage, or with zymotic fluid through temperature control, decompression concentrates, dry, make the Mixed Microbes powder, and then prepare multiple bifidobacterium nutrient health food (crystalline substance with suitable technology with this bacterium powder and different auxiliary material, chew beans, capsule etc.).
2, bifidobacterium nutrient health beverage according to claim 1, food is characterized in that bifid bacterium culture medium prescription is (weight ratio):
2.1 test tube culture medium
Glucose (lactose, maltose, sucrose, fructose) 1.0～3.0
Protein hydrolysate (skimmed milk power, animal blood hydrolysate, beef broth)
1.0～2.0
YE (doing) 0.0～0.5
K
2HPO
4（KH
2PO
4、CaHPO
4） 0.05～0.15
Nacl（Mgcl
2） 0.01～0.10
Water (mineral water, magnetized drinking water, medical stone immersion liquid) 50～65
2.2 glass bottle culture medium
Glucose (maltose, fructose, lactose, sucrose) 1.0～2.0
Skimmed milk power (protein hydrolysate, animal blood hydrolysate) 1.0～2.0
Ground rice (sweet potato flour, Tapioca starch, lotus root starch) 0.0～1.5
Bean powder (soybean, dregs of beans, pea, mung bean flour) 0.0～1.0
Hu Luobu powder 0.3～0.5
Marine alga (sea-tangle, laver, sea mustard powder) 0.0～0.35
YE (doing) 0.0～0.6
Malt flour (carbohydrase, protease) 0.15～0.35
Actinolite extract (doing) (CaHPO
4, Nacl, kcl) 0.01～0.5
Radix polygoni multiflori powder (tuber of dwarf lilyturf, heart of a lotus seed benevolence, Chinese yam, lily, date, radix pseudostellariae etc.)
0.15～2.5
Water (mineral water, magnetized drinking water, medical stone immersion liquid) 50～65
2.3 big jar culture medium
Tapioca starch (ground rice, sweet potato flour, corn flour, lotus root starch) 0.6～2.0
Bean powder (soybean, dregs of beans, pea, mung bean flour) 0.5～1.2
Skimmed milk power (protein hydrolysate, animal blood hydrolysate) 1.0～1.5
Hu Luobu powder 0.3～0.5
Seawood meal (sea-tangle, laver, sea mustard powder) 0.0～0.35
Actinolite extract (CaHPO
4, Nacl, kcl, Mgcl
2)
0.0～0.5
Malt flour (inulase see inulinase, protease) 0.15～0.35
YE (B family vitamin, niacinamide, folic acid) 0.0～0.5
Radix polygoni multiflori powder (tuber of dwarf lilyturf, heart of a lotus seed benevolence, Chinese yam, lily, date, radix pseudostellariae etc.)
0.3～3.5
Water (mineral water, magnetized drinking water, medical stone immersion liquid) 50～65
3, bifidobacterium nutrient health beverage according to claim 1, food is characterized in that its process is:
3.1 the bifid bacterium is cultivated
3.1.1 test tube is cultivated will be by in the 2.1 test tube culture medium injecting tubes of preparing of filling a prescription, through sterilization, temperature control, inoculation, logical CO
2Gas or N
2Gas, airtight, cultivate for 37 ℃ and got the test tube nutrient solution in 20～24 hours.
3.1.2 cultivating, the glass bottle will behind acidifying, enzymolysis, inject the glass bottle again through sterilization, temperature adjustment, inoculation, Anaerobic Treatment by the glass bottle culture medium of prescription 2.2 preparations, airtight, cultivated about 24 hours for 37 ℃, and get seed liquor.
3.1.3 cultivate to fill a prescription 2.3 starch-containing more materials and malt flour and water mixing after after the acidifying, 45 ℃～80 ℃ subsection enzymolysis of temperature control for big jar, adding leftover materials fully stirs, suction filtration, in the culture tank that pumps into pressure sterilizing in advance behind radiation sterilization or high heat (high pressure) instantaneously sterilising, or directly the culture medium behind the suction filtration is pumped into pressure sterilizing in the fermentation tank, temperature adjustment to 35～37 ℃ inoculation 3.0～3.5% above-mentioned seed liquor, logical CO
2Or N
2Gas carries out Anaerobic Treatment, and is airtight, cultivated about 36 hours for 37 ℃, gets zymotic fluid.
3.2 the preparation of bifidobacterium nutrient health beverage, food
3.2.1 bifidobacterium nutrient health beverage can make multiple bifid bacterium health drink with different flavor and characteristics with the zymotic fluid an amount of flavouring agent of adding, sweetener, suspending agent or original fruit juice, vegetable protein liquid after homogeneous is handled through suitable processes.
3.2.2 bifidobacterium nutrient health food
Above-mentioned zymotic fluid is concentrated, makes the Mixed Microbes powder that contains a large amount of bifid bacterium and multiple help material after the drying through decompression, temperature control, with this Mixed Microbes powder and different auxiliary material combination and the suitable PROCESS FOR TREATMENT of warp can make the bifidobacterium nutrient crystalline substance, chew beans, nourishing health-care food such as capsule.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN93107458A CN1096652A (en) | 1993-06-24 | 1993-06-24 | Bifidobacterium nutrient health beverage, food |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN93107458A CN1096652A (en) | 1993-06-24 | 1993-06-24 | Bifidobacterium nutrient health beverage, food |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1096652A true CN1096652A (en) | 1994-12-28 |
Family
ID=4986694
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN93107458A Pending CN1096652A (en) | 1993-06-24 | 1993-06-24 | Bifidobacterium nutrient health beverage, food |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1096652A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102058119A (en) * | 2010-11-04 | 2011-05-18 | 黄冈职业技术学院 | Bahe lotus root powder fermented beverage and production process thereof |
-
1993
- 1993-06-24 CN CN93107458A patent/CN1096652A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102058119A (en) * | 2010-11-04 | 2011-05-18 | 黄冈职业技术学院 | Bahe lotus root powder fermented beverage and production process thereof |
| CN102058119B (en) * | 2010-11-04 | 2012-08-08 | 黄冈职业技术学院 | Bahe lotus root powder fermented beverage and production process thereof |
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| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |