CN109646514A - Longan leaf extract and the preparation method and application thereof - Google Patents
Longan leaf extract and the preparation method and application thereof Download PDFInfo
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- CN109646514A CN109646514A CN201710948747.3A CN201710948747A CN109646514A CN 109646514 A CN109646514 A CN 109646514A CN 201710948747 A CN201710948747 A CN 201710948747A CN 109646514 A CN109646514 A CN 109646514A
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- extract
- ethyl acetate
- longan leaf
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/77—Sapindaceae (Soapberry family), e.g. lychee or soapberry
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Abstract
The invention discloses extract that the extract with alpha-glucosidase activity is inhibited is prepared from longan leaf, the extract is that longan leaf is extracted with EtOH Sonicate after crushed, ethanol extract is condensed into medicinal extract after ethyl alcohol is recovered under reduced pressure, add water that medicinal extract is suspended, the ethyl acetate extract being successively obtained by extraction with petroleum ether, ethyl acetate；And after being extracted with ethyl acetate, then the n-butanol extract obtained with extracting n-butyl alcohol.Show that the active component has through pharmacological experiment study and inhibit alpha-glucosidase activity well, wherein the effect with ethyl acetate extract becomes apparent.The present invention uses abundance, and cheap longan leaf is raw material, provides more extensive Remedies for diabetes source, has good social value and economic value, plays an important role to the hypoglycemic drug for developing efficient, safe.
Description
Technical field
The present invention relates to the preparation of plant extracts and applied technical fields, and in particular to longan leaf extract and its preparation
Method further relates to the application of longan leaf extract.
Background technique
With the variation of continuous improvement of people's living standards and dietary structure, diabetic increases year by year, glycosuria
Disease has become " the third killer " that the mankind are threatened after cardiovascular disease and tumour, has become so preventing and treating diabetes
For global problem.Diabetes are a kind of using hyperglycemia as the metabolic disorder disease of main feature, in pathogenic process most
The symptom first occurred is usually postprandial hyperglycemia, i.e. the early stage sign of diabetes, especially Patients with NIDDM, postprandial high blood
Sugar not only easily induces various complication, can also greatly improve the death rate of diabetes.Therefore, reducing postprandial blood sugar is prevention
Diabetes reduce one of complication and the important measure of the reduction death rate.Blood glucose is mainly derived from carbohydrate and carbon water in food
Compound can be by small intestinal absorption by catalyzing hydrolysis at monosaccharide under the action of alpha-glucosidase.Alpha-glucosidase is sugar
Key enzyme in metabolic process, the effect for controlling alpha-glucosidase can effectively prevent carbohydrate in food and carbohydrate turns
It is melted into monosaccharide and is absorbed, and then reduce blood glucose level.Alpha-glucosidase restrainer mainly passes through inhibition alpha-glucosidase
Activity, the absorption of delay glucose in vivo reduce blood glucose.Therefore, alpha-glucosidase restrainer is currently used for clinic
Oral hypoglycemic agents, such drug clinically has acarbose, voglibose and Miglitol at present.Existing phlorose
Glycosides enzyme inhibitor type is less, and there are certain gastrointestinal side effects, or even can cause certain serious side effects, because
This, safer and more effective natural alpha-glucosidase restrainer requires study exploitation.
Longan leaf is Chinese medicine simply with Guangxi national characters, is recorded in " this sketch of the southern regions of the Yunnan Province is said " earliest.It is famous
Subtropical fruit -- longan (Dimocarpus longan Lour.) tree leaf or tender shoots.Its nature and flavor is sweet, light, flat, has
Deliver the primary efficacies such as heat-clearing, eliminating damp, detoxifying.It is mainly used for treating cold, fever, malaria, furunculosis, eczema etc..Longan originates in
The south subtropics region of south China and North Vietnam has more than 2000 years cultivation histories in China.China's Longan Cultivation area
It occupies first place in the world with yield.South China longan leaf resource reserves are abundant, after annual longan is plucked, orchard worker to trees into
Row lopping, a large amount of leaf are abandoned or burn, and result in waste of resources and environmental pollution.Longan leaf mainly contains tannin, phenolic acid
The ingredients such as class, flavonoids and volatile oil.Although existing research shows that longan leaf has apparent hypoglycemic, the pharmacology such as anti-oxidant
Effect, but it is less about the research report of longan leaf hypoglycemic effect both at home and abroad, and invention once carried out research to its blood sugar reducing function.
The domestic research to longan leaf is reported few at present, in terms of the extraction for being concentrated mainly on flavones ingredient, is had no and is dropped blood for it
Sugared active component or extract extract separation method.Therefore, how by longan leaf play hypoglycemic effect activity at
Divide and extract, to make full use of China's longan leaf resource abundant, researchs and develops more natural alpha-glucosidases suppressions
Preparation is to need the technical issues of conscientiously solving.
The disclosure of background above technology contents is only used for auxiliary and understands inventive concept and technical solution of the invention, not
The prior art for necessarily belonging to present patent application shows above content in the applying date of present patent application in no tangible proof
In the case where having disclosed, above-mentioned background technique should not be taken to the novelty and creativeness of evaluation the application.
Summary of the invention
The purpose of the present invention is to provide a kind of to extract preparation from longan leaf has inhibition alpha-glucosidase activity
Extract and the preparation method and application thereof.
To achieve the above object, the technical solution adopted in the present invention is as follows:
A kind of longan leaf extract, which, which has, inhibits alpha-glucosidase activity.
Further, above-described longan leaf extract is extracted with EtOH Sonicate after crushed for longan leaf, and ethyl alcohol mentions
It is condensed into medicinal extract after taking liquid that ethyl alcohol is recovered under reduced pressure, adds water that medicinal extract is suspended, the second being successively obtained by extraction with petroleum ether, ethyl acetate
Acetoacetic ester extract；And after being extracted with ethyl acetate, then the n-butanol extract obtained with extracting n-butyl alcohol.
Further, above-described longan leaf extract, most preferably preferably ethyl acetate extract.
Further, above-described longan leaf extract, the concentration of alcohol are 50% ethyl alcohol to dehydrated alcohol.
The preparation method of longan leaf extract described in more than one, comprising the following steps:
S1. dry longan leaf is taken, is extracted after crushing with EtOH Sonicate, extracting solution obtains medicinal extract after ethyl alcohol is recovered under reduced pressure；
S2. add water to be suspended medicinal extract, successively extracted with petroleum ether, ethyl acetate, obtain acetic acid ethyl acetate extract；
S3. by acetic acid ethyl acetate extract recycling design, ethyl acetate extract is obtained.
The preparation method of above-described longan leaf extract, further comprising the steps of:
In step S2 S4. the water layer left after " successively being extracted with petroleum ether, ethyl acetate ", then with extracting n-butyl alcohol, obtain
Butanol extraction liquid；
S5. by butanol extraction liquid recycling design, n-butanol extract is obtained.
Further, the preparation method of above-described longan leaf extract, the concentration of alcohol is under step S1
50% ethyl alcohol is to dehydrated alcohol.
Further, the preparation method of above-described longan leaf extract is extracted with EtOH Sonicate under step S1
Successively to be extracted with 95% ethyl alcohol, 50% EtOH Sonicate.
In the application of hypoglycemic aspect, the ethyl acetate extract, n-butanol extract exist above-described longan leaf
The health care product or the application in terms of drug of preparation prevention or treatment diabetes.
In the application of hypoglycemic aspect, the ethyl acetate extract, n-butanol extract exist above-described longan leaf
Preparing has the health care product for inhibiting alpha-glucosidase activity or the application in terms of drug.
The beneficial effects of the present invention are:
1, the raw material used in the present invention are leaf or the tender shoots of longan, and a large amount of longan leaf is discarded or fired in the prior art
It burns, results in waste of resources and environmental pollution, the present invention turn waste into wealth, raw material sources are abundant, cheap.
2, the present invention is screened by modernization extraction separation method in conjunction with a large amount of pharmacological evaluations, preferably obtains having fine
Inhibition alpha-glucosidase activity extract, extract activity is strong, good drug efficacy, and quality safety is controllable.
3, the longan leaf extract with suppression alpha-glucosidase activity provided by the invention can be prepared into drug or health care
Product can facilitate clinical application.The preparation method of longan leaf extract provided by the invention simultaneously, operation is simple, prepares institute
It is high to obtain extract yield, strong operability is suitble to industrialized production.
Detailed description of the invention
Fig. 1 is PNP standard curve.
Fig. 2 is that different extracts compare alpha-glucosaccharase enzyme inhibition activity.
Fig. 3 is influence of the different extract concentrations to alpha-glucosaccharase enzyme inhibition activity.
Specific embodiment
The invention will be further described combined with specific embodiments below, but does not limit the scope of the invention and apply
Range: the one, preparation of longan leaf extract
Embodiment 1
The preparation method of longan leaf extract, comprising the following steps:
S1. dry longan leaf 10kg is taken, is successively extracted with 95% ethyl alcohol and 50% EtOH Sonicate after crushing, is filtered, is merged
Extracting solution is condensed into medicinal extract after ethyl alcohol is recovered under reduced pressure；
S2. it takes medicinal extract that water is added to be suspended, successively respectively extracts 3 times with petroleum ether (60-90 DEG C), ethyl acetate, n-butanol, finally
Remaining aqueous, obtains the corresponding extracting solution of each solvent；
S3. each position extracting solution is taken respectively, solvent is recovered under reduced pressure, and is concentrated, it is dry, longan leaf ligroin extraction is obtained,
Longan leaf ethyl acetate extract, longan leaf n-butanol extract, longan leaf water extract.
Embodiment 2
The preparation method of longan leaf extract, comprising the following steps:
S1. dry longan leaf 10kg is taken, dehydrated alcohol ultrasonic extraction is used after crushing, extracting solution must soak after ethyl alcohol is recovered under reduced pressure
Cream；
S2. it takes ethanol extract that water is added to be suspended, is successively respectively extracted 3 times with petroleum ether (60-90 DEG C), ethyl acetate, n-butanol,
Obtain acetic acid ethyl acetate extract, n-butanol extracting liquid；
S3. it takes acetic acid ethyl acetate extract, n-butanol extracting liquid that solvent is recovered under reduced pressure respectively, is concentrated, it is dry, obtain longan leaf
Ethyl acetate extract, longan leaf n-butanol extract.
Embodiment 3
The preparation method of longan leaf extract, comprising the following steps:
S1. dry longan leaf 10kg is taken, is extracted after crushing with 75% EtOH Sonicate, ethyl alcohol is recovered under reduced pressure in filtering, extracting solution
Medicinal extract is obtained afterwards；
S2. it takes medicinal extract that water is added to be suspended, is successively respectively extracted 3 times with petroleum ether (30-60 DEG C), ethyl acetate, n-butanol, obtain second
Acetoacetic ester extracting solution, n-butanol extracting liquid；
S3. it takes acetic acid ethyl acetate extract, n-butanol extracting liquid that solvent is recovered under reduced pressure respectively, is concentrated, it is dry, obtain longan leaf
Ethyl acetate extract, longan leaf n-butanol extract.
Embodiment 4
The preparation method of longan leaf extract, comprising the following steps:
S1. dry longan leaf 10kg is taken, is extracted after crushing with 50% EtOH Sonicate, ethyl alcohol is recovered under reduced pressure in filtering, extracting solution
Medicinal extract is obtained afterwards；
S2. it takes medicinal extract that water is added to be suspended, is successively respectively extracted 3 times, obtained with petroleum ether (90-120 DEG C), ethyl acetate, n-butanol
Acetic acid ethyl acetate extract, n-butanol extracting liquid；
S3. it takes acetic acid ethyl acetate extract, n-butanol extracting liquid that solvent is recovered under reduced pressure respectively, is concentrated, it is dry, obtain longan leaf
Ethyl acetate extract, longan leaf n-butanol extract.
Embodiment 5
The preparation method of longan leaf extract, comprising the following steps:
S1. dry longan leaf 10kg is taken, is extracted after crushing with 40% EtOH Sonicate, ethyl alcohol is recovered under reduced pressure in filtering, extracting solution
Medicinal extract is obtained afterwards；
S2. it takes medicinal extract that water is added to be suspended, is successively respectively extracted 3 times with petroleum ether (60-90 DEG C), ethyl acetate, n-butanol, obtain second
Acetoacetic ester extracting solution, n-butanol extracting liquid；
S3. it takes acetic acid ethyl acetate extract, n-butanol extracting liquid that solvent is recovered under reduced pressure respectively, is concentrated, it is dry, obtain longan leaf
Ethyl acetate extract, longan leaf n-butanol extract.
Two, longan leaf inhibits the screening study experiment of alpha-glucosidase activity extract
1 instrument and material
Infinite M200PRO microplate reader；(the safe macro medical instrument in Shaoguan is limited for LRH-250-S constant temperature and humidity incubator
Company)；KQ-500DA Ultrasound Instrument (Kunshan Ultrasonic Instruments Co., Ltd.)；HWS-24 electric-heated thermostatic water bath (Shanghai Qi Xinke
Learn Instrument Ltd.)；BSA224S electronic balance；96 microwell plates；Various pipettors and pipette tips；Alpha-glucosidase (G5003-
100UN Sigma company, lot number SLBS9070)；P-nitrophenyl-α-D- glucopyranoside (PNPG, Sigma company, lot number
BCBR9743V)；P-nitrophenol (PNP, Tianjin great Mao chemical reagent factory, lot number: 20170301)；Reduced glutathione
(GSH, Sigma company, lot number 213E053)；Acarbose (Acarbose, Beyer Co., Ltd, lot number BJ33686)；Dimethyl sulfoxide
(DMSO), natrium carbonicum calcinatum, potassium dihydrogen phosphate, dipotassium hydrogen phosphate are that analysis is pure.
2 methods
2.1 sample
Take the longan leaf ligroin extraction of the embodiment of the present invention 1, longan leaf ethyl acetate extract, longan leaf n-butanol
Extract, longan leaf water extract, as sample.
The research of 2.2 alpha-glucosidase activities
Each extract of longan leaf is dissolved with DMSO, and is stored in 4 DEG C of refrigerators, Activity determination is enterprising in 96 microwell plates
Row, reaction system are as follows: taking the 50 μ L of kaliumphosphate buffer of pH6.8, be separately added into 10 μ L of GSH, sample 10 μ L, 0.57U/mL
Alpha-glucosidase 5 μ L, 37 DEG C of isothermal reaction 15min, addition concentration are 20 μ L of 20mmol/L PNPG, 37 DEG C of isothermal reactions
15min adds the NaCO that concentration is 0.2mol/L340 μ L of solution, measures OD value under 400nm wavelength, and each sample is parallel
Measurement 5 times.Using acarbose as positive control, inhibiting rate is calculated as follows:
Inhibition of enzyme activity rate (%)=[ABlank-(ASample-ABackground)]/ABlank×100
Note: ABlank: the light absorption value of reaction is added in no sample；ASample: the light absorption value of example reaction is added；ABackground: no enzyme solution is added
Light absorption value.Inhibiting rate is calculated, and finds out corresponding IC with SPSS22.0 software50Value.
The drafting of 2.3 standard curves
According to the reaction system of use, 1000 μm of ol/L PNP are configured with kaliumphosphate buffer (pH6.8), are diluted to respectively
400μmol/L,300μmol/L,200μmol/L,100μmol/L,80μmol/L,40μmol/L,20μmol/L,0μmol/L.Point
Each 160 μ L of the PNP solution of 9 kinds of various concentrations is not taken, and 0.2mol/L NaCO is added380 μ L of solution is mixed, under 400nm wavelength
OD value is measured, 3 groups is surveyed and is averaged.Using OD value as ordinate, PNP concentration is abscissa, does standard curve.
3 results
The drafting of 3.1 standard curves
The regression equation of PNP standard working curve is y=0.0072x+0.0989, R2=0.9995, illustrate PNP in 0 μ
Good linear relationship is presented in mol/L~1000 μm ol/L and OD value.The result is shown in Figure 1.
The expression activitiy of 3.2 longan leaf difference extracts
As shown in Table 1, at the same concentration (0.5mg/ml), using acarbose as positive control, alpha-glucosidase suppression
Activity Results processed show that longan leaf ethyl acetate extract, n-butanol extract inhibiting rate respectively reach 69.98% (IC50=
29.9mg/L), 67.02% (IC50=41mg/L), it is above 64.58% (IC of positive control drug acarbose50=73.8mg/
L).Longan leaf opposed polarity extract alpha-glucosaccharase enzyme inhibition activity is followed successively by ethyl acetate extract > positive fourth from big to small
Alcohol extracting thing > acarbose > ligroin extraction > water extract.It the results are shown in Table 1 and Fig. 2.
The different extract alpha-glucosaccharase enzyme inhibition activities (n=5) of table 1
Influence of the 3.3 longan leaf difference extract different quality concentration to alpha-glucosaccharase enzyme inhibition activity
Influence of the longan leaf difference extract different quality concentration to alpha-glucosaccharase enzyme inhibition activity the result shows that, 4
Different extracts are in positive dose-effect relationship, i.e. dose dependent to alpha-glucosaccharase enzyme inhibition activity and concentration, and inhibitory activity is with dense
Degree improves and enhances, and wherein the concentration of ethyl acetate extract is linearly to increase in 0.1~1mg/mL, when concentration increases to one
Periodically, concentration only has very little variation.Within the scope of experimental concentration, the inhibiting rate of acarbose also increases with the increase of concentration,
And inhibiting rate is higher than positive control inhibiting rate within this range for longan leaf ethyl acetate extract and n-butanol extract.As a result
It is shown in Table 2 and Fig. 3.
Influence (n=5) of the 2 longan leaf difference extract various concentration of table to alpha-glucosaccharase enzyme inhibition activity
Through experiment it is found that in the opposed polarity extract of longan leaf, ethyl acetate extract and n-butanol extract
It is stronger to alpha-glucosaccharase enzyme inhibition activity, it is eager to excel than the inhibiting effect of acarbose.
Three, influence of the embodiment of the present invention to alpha-glucosaccharase enzyme inhibition activity
On the basis of longan leaf inhibits the screening study experiment of alpha-glucosidase activity extract, the present invention is implemented
The resulting ethyl acetate extract of example 1-5 and n-butanol extract compare research to alpha-glucosaccharase enzyme inhibition activity.
1 instrument and material
Inhibit the screening study experiment of alpha-glucosidase activity extract with longan leaf
2 methods
2.1 sample
Take the longan leaf ethyl acetate extract of 1-5 of the embodiment of the present invention, longan leaf n-butanol extract, as sample.
The research of 2.2 alpha-glucosidase activities
Inhibit the screening study experiment of alpha-glucosidase activity extract with longan leaf.
The drafting of 2.3 standard curves
Inhibit the screening study experiment of alpha-glucosidase activity extract with longan leaf.
3 results
The drafting of 3.1 standard curves
See that longan leaf inhibits the screening study experiment of alpha-glucosidase activity extract.
The influence to alpha-glucosaccharase enzyme inhibition activity of extract is compared in 3.2 embodiment 1-5
As shown in Table 3, at the same concentration (0.5mg/ml), using acarbose as positive control, alpha-glucosidase suppression
Activity Results processed show that the ethyl acetate extract inhibiting rate of embodiment 1-4 respectively reaches 69.98% (IC50=29.9mg/L),
67.53% (IC50=37.7mg/L), 68.18% (IC50=30.3mg/L), 66.92% (IC50=50.1mg/L), it is above
64.58% (IC of positive control drug acarbose50=73.8mg/L), and the ethyl acetate extract inhibiting rate of embodiment 5 is
63.42% (IC50=79.2mg/L), it is lower than positive control drug acarbose.It the results are shown in Table 3.
Ethyl acetate extract alpha-glucosaccharase enzyme inhibition activity (n=5) in each embodiment of table 3
As shown in Table 4, at the same concentration (0.5mg/ml), using acarbose as positive control, alpha-glucosidase suppression
Activity Results processed show that the n-butanol extract inhibiting rate of embodiment 1-4 respectively reaches 67.02% (IC50=41.0mg/L),
66.06% (IC50=51.0mg/L), 66.87% (IC50=44.5mg/L), 65.83% (IC50=61.3mg/L), it is above
64.58% (IC of positive control drug acarbose50=73.8mg/L), and the n-butanol extract inhibiting rate of embodiment 5 is
62.15% (IC50=85.3mg/L), it is lower than positive control drug acarbose.It the results are shown in Table 4.
N-butanol extract alpha-glucosaccharase enzyme inhibition activity (n=5) in each embodiment of table 4
It can be seen from the above result that the ethyl acetate extract and n-butanol extract of 1-4 of the embodiment of the present invention are to α-grape
Glucoside inhibiting activity is relatively strong, is eager to excel than the inhibiting effect of acarbose, and the ethyl acetate extract of embodiment 5 and just
Butanol extract is relatively lower than acarbose to alpha-glucosaccharase enzyme inhibition activity, illustrates the extract of 40% ethyl alcohol extraction to α-
Glucosidase inhibitory active is lower, and the extract that 50% or more ethyl alcohol extracts is equal to alpha-glucosaccharase enzyme inhibition activity
It is higher, therefore the ethyl alcohol of present invention selection 50% or more extracts.
Claims (10)
1. a kind of longan leaf extract, which is characterized in that the extract, which has, inhibits alpha-glucosidase activity.
2. longan leaf extract according to claim 1, which is characterized in that the extract is that longan leaf uses second after crushed
Alcohol ultrasonic extraction, ethanol extract are condensed into medicinal extract after ethyl alcohol is recovered under reduced pressure, add water that medicinal extract is suspended, and successively use petroleum ether, second
The ethyl acetate extract that acetoacetic ester is obtained by extraction；And after being extracted with ethyl acetate, then the n-butanol obtained with extracting n-butyl alcohol
Extract.
3. longan leaf extract according to claim 2, which is characterized in that the extract is ethyl acetate extract.
4. longan leaf extract according to claim 2, which is characterized in that the concentration of alcohol is 50% ethyl alcohol to anhydrous
Ethyl alcohol.
5. a kind of preparation method of longan leaf extract as described in claim 1, which comprises the following steps:
S1. dry longan leaf is taken, is extracted after crushing with EtOH Sonicate, extracting solution obtains medicinal extract after ethyl alcohol is recovered under reduced pressure；
S2. add water to be suspended medicinal extract, successively extracted with petroleum ether, ethyl acetate, obtain acetic acid ethyl acetate extract；
S3. by acetic acid ethyl acetate extract recycling design, ethyl acetate extract is obtained.
6. a kind of preparation method of longan leaf extract as claimed in claim 5, which is characterized in that further comprising the steps of:
In step S2 S4. the water layer left after " successively being extracted with petroleum ether, ethyl acetate ", then with extracting n-butyl alcohol, obtain positive fourth
Alcohol extract liquor；
S5. by butanol extraction liquid recycling design, n-butanol extract is obtained.
7. the preparation method of longan leaf extract according to claim 5 or 6, which is characterized in that the concentration of alcohol is
50% ethyl alcohol is to dehydrated alcohol.
8. the preparation method of longan leaf extract according to claim 5 or 6, which is characterized in that use second under step S1
Alcohol ultrasonic extraction is successively to be extracted with 95% ethyl alcohol, 50% EtOH Sonicate.
9. longan leaf as described in claim 1 is in the application of hypoglycemic aspect, it is characterised in that: the ethyl acetate extracts
The application of object, n-butanol extract in terms of the health care product or drug of preparation prevention or treatment diabetes.
10. longan leaf as described in claim 1 is in the application of hypoglycemic aspect, it is characterised in that: the ethyl acetate extracts
The application of object, n-butanol extract in terms of preparing the health care product or drug with inhibition alpha-glucosidase activity.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110433220A (en) * | 2019-07-30 | 2019-11-12 | 浙江师范大学 | The method of alpha-glucosidase restrainer is extracted from longan seed |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1903053A (en) * | 2005-07-25 | 2007-01-31 | 王浩贵 | Tea contg. longan tree leaves for treating cold |
| CN104351442A (en) * | 2014-12-03 | 2015-02-18 | 大新县科学技术情报研究所 | Longan leaf heat-clearing and detoxifying tea |
-
2017
- 2017-10-12 CN CN201710948747.3A patent/CN109646514A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1903053A (en) * | 2005-07-25 | 2007-01-31 | 王浩贵 | Tea contg. longan tree leaves for treating cold |
| CN104351442A (en) * | 2014-12-03 | 2015-02-18 | 大新县科学技术情报研究所 | Longan leaf heat-clearing and detoxifying tea |
Non-Patent Citations (2)
| Title |
|---|
| 梁洁等: "龙眼叶不同提取物降血糖的实验研究 ", 《时珍国医国药》 * |
| 贤景春等: "龙眼核提取物的α-葡萄糖苷酶抑制活性体外实验的研究", 《食品科技》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110433220A (en) * | 2019-07-30 | 2019-11-12 | 浙江师范大学 | The method of alpha-glucosidase restrainer is extracted from longan seed |
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Application publication date: 20190419 |