CN109645112A - A kind of bactericidal composition and its application of probenazole-containing and procymidone - Google Patents
A kind of bactericidal composition and its application of probenazole-containing and procymidone Download PDFInfo
- Publication number
- CN109645112A CN109645112A CN201910122374.3A CN201910122374A CN109645112A CN 109645112 A CN109645112 A CN 109645112A CN 201910122374 A CN201910122374 A CN 201910122374A CN 109645112 A CN109645112 A CN 109645112A
- Authority
- CN
- China
- Prior art keywords
- procymidone
- probenazole
- bactericidal composition
- agent
- dosage form
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- WHHIPMZEDGBUCC-UHFFFAOYSA-N probenazole Chemical compound C1=CC=C2C(OCC=C)=NS(=O)(=O)C2=C1 WHHIPMZEDGBUCC-UHFFFAOYSA-N 0.000 title claims abstract description 82
- QXJKBPAVAHBARF-BETUJISGSA-N procymidone Chemical compound O=C([C@]1(C)C[C@@]1(C1=O)C)N1C1=CC(Cl)=CC(Cl)=C1 QXJKBPAVAHBARF-BETUJISGSA-N 0.000 title claims abstract description 79
- 239000000203 mixture Substances 0.000 title claims abstract description 47
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 38
- 238000003860 storage Methods 0.000 claims abstract description 25
- 244000052769 pathogen Species 0.000 claims abstract description 18
- 230000001717 pathogenic effect Effects 0.000 claims abstract description 18
- 239000004530 micro-emulsion Substances 0.000 claims abstract description 14
- 241000123650 Botrytis cinerea Species 0.000 claims abstract description 13
- 235000012055 fruits and vegetables Nutrition 0.000 claims abstract description 13
- 201000010099 disease Diseases 0.000 claims abstract description 12
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 12
- 241000193738 Bacillus anthracis Species 0.000 claims abstract description 11
- 239000000463 material Substances 0.000 claims abstract description 11
- 239000000375 suspending agent Substances 0.000 claims abstract description 11
- 230000002265 prevention Effects 0.000 claims abstract description 10
- 239000002552 dosage form Substances 0.000 claims abstract description 9
- 239000000839 emulsion Substances 0.000 claims abstract description 8
- 239000000575 pesticide Substances 0.000 claims abstract description 8
- 239000004563 wettable powder Substances 0.000 claims abstract description 8
- 239000004562 water dispersible granule Substances 0.000 claims abstract description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- YTPLMLYBLZKORZ-UHFFFAOYSA-N Thiophene Chemical compound C=1C=CSC=1 YTPLMLYBLZKORZ-UHFFFAOYSA-N 0.000 claims description 10
- 241000894006 Bacteria Species 0.000 claims description 9
- 241000555706 Botryosphaeria dothidea Species 0.000 claims description 6
- 239000012752 auxiliary agent Substances 0.000 claims description 6
- 229930192474 thiophene Natural products 0.000 claims description 6
- 230000000857 drug effect Effects 0.000 claims description 3
- 230000006641 stabilisation Effects 0.000 claims description 3
- 238000011105 stabilization Methods 0.000 claims description 3
- 239000000080 wetting agent Substances 0.000 claims description 3
- 239000003463 adsorbent Substances 0.000 claims description 2
- 230000002528 anti-freeze Effects 0.000 claims description 2
- 239000002518 antifoaming agent Substances 0.000 claims description 2
- 239000002270 dispersing agent Substances 0.000 claims description 2
- 239000003995 emulsifying agent Substances 0.000 claims description 2
- 239000002904 solvent Substances 0.000 claims description 2
- 239000002131 composite material Substances 0.000 claims 1
- 235000013399 edible fruits Nutrition 0.000 abstract description 14
- 230000002195 synergetic effect Effects 0.000 abstract description 12
- 235000013305 food Nutrition 0.000 abstract description 7
- 239000000417 fungicide Substances 0.000 abstract description 7
- 235000013311 vegetables Nutrition 0.000 abstract description 7
- 230000000694 effects Effects 0.000 abstract description 6
- 230000000855 fungicidal effect Effects 0.000 abstract description 6
- 206010064458 Penicilliosis Diseases 0.000 abstract description 5
- 230000010534 mechanism of action Effects 0.000 abstract description 3
- 238000013329 compounding Methods 0.000 abstract 1
- 241000220225 Malus Species 0.000 description 15
- 235000011430 Malus pumila Nutrition 0.000 description 15
- 235000015103 Malus silvestris Nutrition 0.000 description 15
- 239000003814 drug Substances 0.000 description 14
- 238000002360 preparation method Methods 0.000 description 14
- 240000002234 Allium sativum Species 0.000 description 13
- 235000004611 garlic Nutrition 0.000 description 13
- 238000012545 processing Methods 0.000 description 13
- 238000000034 method Methods 0.000 description 9
- 230000002401 inhibitory effect Effects 0.000 description 7
- 230000001988 toxicity Effects 0.000 description 7
- 231100000419 toxicity Toxicity 0.000 description 7
- 231100000820 toxicity test Toxicity 0.000 description 7
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 239000000470 constituent Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 241000233866 Fungi Species 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- TWFZGCMQGLPBSX-UHFFFAOYSA-N carbendazim Chemical compound C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 239000003921 oil Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 239000005820 Prochloraz Substances 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 239000003899 bactericide agent Substances 0.000 description 3
- 238000005260 corrosion Methods 0.000 description 3
- 238000007598 dipping method Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000002386 leaching Methods 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 230000003285 pharmacodynamic effect Effects 0.000 description 3
- TVLSRXXIMLFWEO-UHFFFAOYSA-N prochloraz Chemical compound C1=CN=CN1C(=O)N(CCC)CCOC1=C(Cl)C=C(Cl)C=C1Cl TVLSRXXIMLFWEO-UHFFFAOYSA-N 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- PXMNMQRDXWABCY-UHFFFAOYSA-N 1-(4-chlorophenyl)-4,4-dimethyl-3-(1H-1,2,4-triazol-1-ylmethyl)pentan-3-ol Chemical compound C1=NC=NN1CC(O)(C(C)(C)C)CCC1=CC=C(Cl)C=C1 PXMNMQRDXWABCY-UHFFFAOYSA-N 0.000 description 2
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 241000222199 Colletotrichum Species 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 2
- 244000081841 Malus domestica Species 0.000 description 2
- 239000005839 Tebuconazole Substances 0.000 description 2
- 238000012271 agricultural production Methods 0.000 description 2
- 235000021016 apples Nutrition 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 239000000945 filler Substances 0.000 description 2
- 230000009036 growth inhibition Effects 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 238000012417 linear regression Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000036961 partial effect Effects 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000003672 processing method Methods 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- HYZJCKYKOHLVJF-UHFFFAOYSA-N 1H-benzimidazole Chemical compound C1=CC=C2NC=NC2=C1 HYZJCKYKOHLVJF-UHFFFAOYSA-N 0.000 description 1
- -1 3,5- dichlorophenyl Chemical group 0.000 description 1
- 244000000383 Allium odorum Species 0.000 description 1
- 235000018645 Allium odorum Nutrition 0.000 description 1
- 244000144730 Amygdalus persica Species 0.000 description 1
- 241000221198 Basidiomycota Species 0.000 description 1
- 241001465180 Botrytis Species 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 235000016623 Fragaria vesca Nutrition 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 1
- 239000005867 Iprodione Substances 0.000 description 1
- 229920001732 Lignosulfonate Polymers 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 239000005828 Pyrimethanil Substances 0.000 description 1
- 241000220324 Pyrus Species 0.000 description 1
- 241000221662 Sclerotinia Species 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 235000002597 Solanum melongena Nutrition 0.000 description 1
- 102000004243 Tubulin Human genes 0.000 description 1
- 108090000704 Tubulin Proteins 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 235000010724 Wisteria floribunda Nutrition 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 150000001556 benzimidazoles Chemical class 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000006013 carbendazim Substances 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000000890 drug combination Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000013213 extrapolation Methods 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- ONUFESLQCSAYKA-UHFFFAOYSA-N iprodione Chemical compound O=C1N(C(=O)NC(C)C)CC(=O)N1C1=CC(Cl)=CC(Cl)=C1 ONUFESLQCSAYKA-UHFFFAOYSA-N 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 239000008297 liquid dosage form Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000003595 mist Substances 0.000 description 1
- 230000011278 mitosis Effects 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 230000005405 multipole Effects 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 239000002420 orchard Substances 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 235000021017 pears Nutrition 0.000 description 1
- 230000004260 plant-type cell wall biogenesis Effects 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- ZLIBICFPKPWGIZ-UHFFFAOYSA-N pyrimethanil Chemical compound CC1=CC(C)=NC(NC=2C=CC=CC=2)=N1 ZLIBICFPKPWGIZ-UHFFFAOYSA-N 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 239000007909 solid dosage form Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 239000004546 suspension concentrate Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 150000003577 thiophenes Chemical class 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- 150000003626 triacylglycerols Chemical class 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses the bactericidal composition of a kind of probenazole-containing and procymidone, effective component is probenazole and procymidone, and the weight ratio of probenazole and procymidone is 15:1~1:10, preferably 10:1~1:5, further preferred 5:1~1:3, still further preferably 3:1~1:2.Can also containing allow in Pesticide Science using with acceptable auxiliary material.Dosage form can be wettable powder, suspending agent, aqueous emulsion, water dispersible granules, microemulsion.The application of bactericidal composition of the invention in prevention and treatment fruit, vegetable storage phase disease, the disease are selected from gray mold, anthracnose, ring spot, penicilliosis.Bactericidal composition of the invention, effective component probenazole, the mechanism of action of procymidone are entirely different, compounding has obvious synergistic function, can be improved to the pathogen of Botrytis cinerea, Target spot pathogen, anthrax bacteria bactericidal activity, delay the generation of fungicide resistance, keep the corrosion-resistanting fresh-keeping effect of fruit and vegetable food more significant, improves the economic value of fruit and vegetable food.
Description
Technical field
The present invention relates to the bactericidal compositions of a kind of probenazole-containing and procymidone, and its in prevention and treatment fruit, vegetable storage phase
Application in disease belongs to pesticide control technical field.
Background technique
Fresh agricultural products storage logistics is the continuity of agricultural production, keeps its excellent quality and freshness is people's pursuit
One of important goal, postharvest decay is rotten must to be solved the problems, such as during fresh agricultural products storage, logistics transportation.According to system
Meter, the loss late in developed country's veterinary antibiotics product transporting procedures less than 5%, and the annual loss late in China be up to 25%~
30%.The pathogen of Botrytis cinerea (Botrytis cinerea), anthrax bacteria (Colletotrichum gloeosporiorides) are
Cause the important pathogen that postharvest fruit and vegetable rots, grape, strawberry, peach, apple, pears, tomato, cucumber, eggplant, fragrant-flowered garlic can be endangered
The various fruits such as dish, vegetables not only cause to seriously endanger and the important disease of storage period in fruits and vegetables growth period.
Prevented and treated in production the pathogen of Botrytis cinerea, the common chemical bactericide of anthrax bacteria have carbendazim, iprodione, pyrimethanil,
Prochloraz etc., but the pathogen of Botrytis cinerea, anthrax bacteria have the characteristics that breeding is fast, hereditary variation is big, grade of fit is high, and continuous use is same
One medicament is also easy to produce drug resistance, brings extreme difficulties to prevention and treatment, only controls fruits and vegetables by increasing dosing or times for spraying and produces
Product rot, and are either unfavorable for improving anti-corrosion effect still to guarantee fruits and vegetables quality all.Meanwhile as people are to food
The residual toxicity of the concern of product safety problem, fungicide is increasingly taken seriously, and it is few to find safer, wide spectrum, dosage
Germ killing drugs combination, reduces the usage amount of chemical bactericide, delays the generation of drug resistance, be current postharvest fruit and vegetable anti-corrosive fresh-keeping
Research hotspot and the technical problem to be solved in the present invention.
Probenazole, belongs to benzimidazoles fungicide, and mechanism of action is the tubulin inhibited during fungi mitosis
Formed, the lasting period is long, for prevent and treat crops, the industrial crops various diseases as caused by sac fungus, basidiomycetes and Fungi Imperfecti and
Antiseptic preservation of fruits and vegetables, be it is a kind of efficiently, wide spectrum, in the world general fungicide.
Procymidone belongs to hypotoxicity fungicide, entitled N- (3,5- dichlorophenyl) -1, the 2- dimethylcyclopropane-of chemistry
1,2- diformyl imines mainly acts on cell membrane suppression for protectiveness and curative fungicide agent with certain systemic action
The synthesis of triglycerides in thallus processed hinders normal cell wall synthesis in mycelia top to inhibit the development of mycelia.To Botrytis and
Sclerotinia fungi has good result, can prevent and treat fruit tree, the gray mold of vegetable crop, sclerotiniose, generates resistance to benzimidazole
Fungi it is also effective.
Probenazole or procymidone is used alone to be prevented and be controlled fruit, vegetable storage phase gray mold, anthracnose, wheel
Line disease, penicilliosis effect are undesirable.Currently, there is not yet the relevant report that procymidone is compounded with probenazole.
Summary of the invention
For the above-mentioned prior art, the present invention provides the bactericidal compositions of a kind of probenazole-containing and procymidone, and its
Application in prevention and treatment fruit, vegetable storage phase disease (especially gray mold, anthracnose, ring spot, penicilliosis).
The present invention is achieved by the following technical solutions:
A kind of bactericidal composition of probenazole-containing and procymidone, effective component are probenazole and procymidone, probenazole and rotten
The weight ratio of mould benefit is 15:1~1:10, preferably 10:1~1:5, further preferred 5:1~1:3, still further preferably 3:1~1:
2。
Further, in the bactericidal composition of the probenazole-containing and procymidone, can containing allow in Pesticide Science using
With acceptable auxiliary material.
Further, the bactericidal composition of the probenazole-containing and procymidone is grouped as by the group of following parts by weight: thiophene
Bacterium spirit 1%~80%, procymidone 1%~80%, surplus be Pesticide Science in allow using with acceptable auxiliary material.
Allow to be selected from solvent, dispersing agent, diffusant, disintegrating agent, cream using with acceptable auxiliary material in the Pesticide Science
Agent, adsorbent, stabilizer, wetting agent, defoaming agent, antifreeze etc. or other stabilizations for facilitating effective component drug effect and hair
That waves allows using auxiliary agent.
The bactericidal composition of the probenazole-containing and procymidone can be prepared into processing method well known in the prior art
Any one dosage form that suitable agricultural production uses, preferred dosage form are as follows: wettable powder, suspending agent, aqueous emulsion, water-dispersible grain
Agent, microemulsion.
The bactericidal composition of probenazole-containing and procymidone of the invention, answering in prevention and treatment fruit, vegetable storage phase disease
With.The disease is selected from gray mold, anthracnose, ring spot, penicilliosis.
Further, when preventing and treating the pathogen of Botrytis cinerea, the weight ratio of probenazole and both procymidones is with 10:1~1:3
Good, more preferably, 1:1 is best by 2:1~1:1；When preventing and treating Botryosphaeria berengeriana f. sp, the weight ratio of both probenazole and procymidone is with 5:1
~1:3 is preferred, and more preferably, 3:1 is best by 3:1~1:1；When preventing and treating apple anthrax bacteria, the weight of both probenazole and procymidone
Than being preferred with 5:1~1:5, more preferably, 1:1 is best by 2:1~1:2.
The bactericidal composition of probenazole-containing and procymidone of the invention can be provided in the form of finished product preparation, can also be with single dose
Form provides, and using preceding direct mixing, is then watered mixing and is made into required concentration, dipping or spray before water fruits and vegetables enter freezer
Mist is put in storage after drying.
The bactericidal composition of probenazole-containing and procymidone of the invention, the mechanism of action of effective component probenazole, procymidone
Entirely different, procymidone and probenazole (are used in combination by test, screen the basis with field trial indoors by inventor
On, the anti-corrosion effect to storage period gray mold, anthracnose, ring spot, penicilliosis is measured, there is apparent synergistic effect) discovery,
Probenazole is compounded with procymidone, has the advantage that (1) apparent synergistic function, the composition improves thiophene
Bacterium spirit to the pathogen of Botrytis cinerea, Target spot pathogen, anthrax bacteria bactericidal activity；(2) reduce the usage amount of chemical bactericide and make
With number, use cost is reduced, the quality safety of food can be improved；(3) drug combination of different role mechanism uses, and can prolong
The generation of slow fungicide resistance；(4) corrosion-resistanting fresh-keeping effect of fruit and vegetable food is more significant, substantially prolongs the goods of fruit and vegetable food
The frame phase improves the economic value of fruit and vegetable food.
The various terms and phrase that the present invention uses are with well known to a person skilled in the art general senses.The term referred to
With phrase if any meaning inconsistent with common art-recognized meanings, that the present invention of being subject to is stated.
Detailed description of the invention
The pharmacodynamic results of Fig. 1: 30% probenazole procymidone microemulsion prevention and treatment storage period apple anthracnose.
Specific embodiment
Below with reference to embodiment, the present invention is further illustrated.However, the scope of the present invention is not limited to following realities
Apply example.One of skill in the art, can be to the present invention it is understood that under the premise of without departing substantially from the spirit and scope of the present invention
Carry out various change and modification.
The present invention carries out general and/or specific description to the material and test method arrived used in test.Though
So many materials and operating method used in purpose are it is known in the art that still the present invention is still herein to realize the present invention
It is described in detail as far as possible.
Instrument involved in following embodiments, reagent, material etc. are unless otherwise noted existing in the prior art
Conventional instrument, reagent, material etc., can be obtained by regular commercial sources.Experimental method involved in following embodiments, inspection
Survey method etc. is unless otherwise noted existing routine experiment method in the prior art, detection method etc..
Experiment probenazole is compounded with procymidone studies (indoor toxicity test) to the preventive and therapeutic effect of disease
One, reagent agent: probenazole, procymidone raw medicine.By probenazole, procymidone raw medicine acetone solution, and it is made into
Emulsifier Tween-80 is added with 0.2% volume fraction in the mother liquor of 10000 μ g/mL, stores in refrigerator spare at 4 DEG C, makes
Used time is diluted to debita spissitudo with sterile water.
Two, for trying pathogen: Pathogen Botrytis cinerea Pers. ex of Garlic Sprout (Botrytis cinerea), Botryosphaeria berengeriana f. sp
(Botryosphaeria dothidea), apple anthrax bacteria (Colletotrichum gloeosporiorides).
Three, indoor toxicity test method: using mycelial growth rate method to probenazole, procymidone and the two different ratio group
It closes and carries out toxicity test, and calculate co-toxicity coefficient (CTC).
Single agent virulence determination method: reagent agent (including probenazole, procymidone) is first configured to 5 concentration gradient (thiophenes
Bacterium spirit: 4.0,2.0,1.0,0.5,0.25mg/kg；Procymidone: 10.0,5.0,2.5,1.25,0.625mg/kg), by pathogen
In 26 DEG C of preculture 3d in PDA culture medium, is beaten on the same circumference close to colony edge with the punch of diameter 7mm and take bacterium
Cake is inoculated on the culture medium flat plate containing different agents concentration, and every ware is a piece of, is placed in 26 DEG C of insulating boxs and is cultivated 3d, with ten
Word interior extrapolation method measures the colony growth diameter of each processing, is control with clear water, and every concentration processing is repeated 4 times.It is radial to measure bacterium colony
Linear growth amount determines the inhibiting rate that medicament grows bacterium colony.Pass through mycelia growth inhibition probability value and drug concentration logarithm
Between linear regression analysis, find out each medicament to bacterial strain it is effective inhibition in concentration (EC50Value).
Mixture joint toxicity measuring: it on the basis of single dose toxicity test, will be pressed respectively for the probenazole of mixture, procymidone
Its EC50Example is respectively set 15:1,10:1,5:1,3:1,2:1,1:1,1:2,1:3,1:5,1:10 totally 10 to value dosage by volume
Proportion, by different ratio be configured to final mass concentration be 2.0,1.0,0.5,0.25,0.125mg/kg 5 series of concentrations
Drug containing PDA culture medium, the inhibiting rate that measurement medicament grows mycelia.Every processing (every every concentration level of bacterial strain) is repeated 4 times.Pass through
Linear regression analysis between mycelia growth inhibition probability value and drug concentration logarithm finds out Mixed Pharmacy to the effective of bacterial strain
Concentration EC in inhibition50Value and CTC value, using the PDA culture medium of not adding medicine as control.
Four, data statistic analysis
Test data is for statistical analysis by Microsoft Excel 2013, DPS data processing work platform, calculates
The EC of every kind of medicament out50Value, 95% confidence limit.The co-toxicity coefficient that mixed agent is calculated using Sun Yunpei's Method (1960), according to total poison
The synergistic effect of coefficient magnitude evaluation mixed agent.
Each medicament inhibiting rate is calculated according to the following formula:
Inhibit growth rate (%)=[(control antibacterial circle diameter-processing antibacterial circle diameter)/(control antibacterial circle diameter -5mm
Punch diameter)] × 100；
Inhibit growth rate (%)=[(control colony diameter-processing colony diameter)/(control colony diameter-bacteria cake diameter)]
×100；
Toxicity index TI=(standard agent EC50/ reagent agent EC50)×100；
Mixed agent actual toxicity index ATI=(standard agent EC50/ mixture preparation EC50)×100；
(PA and PB are respectively mixed agent to TI × PB of TI × PA+ single dose B of mixed agent theoretical toxicity index TTI=single dose A
The percentage composition of middle effective component)；
Theoretical toxicity index TTI × 100 of the actual toxicity index ATI/ mixed agent of co-toxicity coefficient CTC=mixed agent；
Synergistic effect judgement: CTC >=120 have synergistic effect；80 < CTC < 120 are summation action；CTC≤80 are
Antagonism.
Five, result and analysis: as shown in Table 1 to Table 3.
1 probenazole of table is with procymidone different proportion mixture to the toxicity test result of the pathogen of Botrytis cinerea
Seen from table 1, probenazole, procymidone and its mixture preparation have good inhibiting effect to the pathogen of Botrytis cinerea,
When the proportion of middle probenazole and procymidone is between 10:1~1:3, to the co-toxicity coefficient for trying pathogen 120 or more, table
Both bright compositions have good synergistic function under this proportion, and especially proportion is between 5:1~1:3, EC50Value
The EC being used alone significantly lower than probenazole and procymidone50Value, mixture preparation significantly propose the inhibitory activity of the pathogen of Botrytis cinerea
Height, for co-toxicity coefficient (CTC) value up to 141.18~249.60, synergistic effect is more significant.
2 probenazole of table is with procymidone different proportion mixture to the toxicity test result of Botryosphaeria berengeriana f. sp
As can be seen from Table 2, probenazole, procymidone and its mixture preparation, have good inhibiting effect to Botryosphaeria berengeriana f. sp,
When wherein the proportion of probenazole and procymidone is between 5:1~1:3, to the co-toxicity coefficient for trying pathogen 120 or more, table
Both bright compositions have good synergistic function under this proportion, and especially proportion is between 3:1~1:1, mixture
The EC of preparation50The EC that value is used alone significantly lower than probenazole and procymidone50Value, co-toxicity coefficient (CTC) value up to 156.82~
197.51 synergistic effect is more significant.
3 probenazole of table is with procymidone different proportion mixture to the toxicity test result of apple anthrax bacteria
Seen from table 3, probenazole, procymidone and its mixture preparation have good inhibiting effect to apple anthrax bacteria,
When wherein the proportion of probenazole and procymidone is between 5:1~1:5, to the co-toxicity coefficient for trying pathogen 120 or more, table
Both bright compositions have good synergistic function under this proportion, and especially proportion is between 5:1~1:2, mixture
The EC of preparation50The EC that value is used alone significantly lower than probenazole and procymidone50Value, co-toxicity coefficient (CTC) value up to 150.68~
218.13 synergistic effect is more significant.
1 21% probenazole procymidone aqueous emulsion of embodiment
Group is grouped as following (each component is by weight percentage):
The preparation method comprises the following steps: above-mentioned each active constituent, various auxiliary agents are sufficiently mixed in proportion, make to be dissolved into homogeneous oil phase,
Under high-speed stirred, oil is mutually mixed with water, obtains 21% probenazole procymidone aqueous emulsion.
2 18% probenazole procymidone water dispersible granules of embodiment
Group is grouped as following (each component is by weight percentage):
The preparation method comprises the following steps: above-mentioned each active constituent, lignosulfonates, wetting agent, disintegrating agent, filler is abundant in proportion
Mixing, be crushed to 5 μm of partial size through airslide disintegrating mill, obtain female powder, by female powder with alkylphenol-polyethenoxy is phonetic be sufficiently mixed after be granulated,
Drying, sieving, so that 18% probenazole procymidone water dispersible granules be made.
3 30% probenazole procymidone suspending agent of embodiment
Group is grouped as following (each component is by weight percentage):
The preparation method comprises the following steps: above-mentioned each active constituent, various auxiliary agents and water are sufficiently mixed uniformly in proportion, investment is sanded
Machine is ground to 5 μm of partial size, and 30% probenazole procymidone suspending agent is made.
4 40% probenazole procymidone wettable powder of embodiment
Group is grouped as following (each component is by weight percentage):
The preparation method comprises the following steps: above-mentioned each active constituent, various auxiliary agents and filler are sufficiently mixed uniformly in proportion, mixture warp
40% probenazole procymidone wettable powder is made in ultra-fine pulverizer disintegrating.
5 30% probenazole procymidone microemulsion of embodiment
Group is grouped as following (each component is by weight percentage):
The preparation method comprises the following steps: above-mentioned each active constituent, various auxiliary agents are sufficiently mixed in proportion, make to be dissolved into homogeneous oil phase,
Under high-speed stirred, oil is mutually mixed with water, obtains 30% probenazole procymidone microemulsion.
Application example 1:30% probenazole procymidone microemulsion prevents and treats the research of storage period apple anthracnose
On October 22nd, 2017, kind was Fuji in the Qufu City Wu villages and small towns apple orchard Wu Cun, and selection nothing is rotted, without machinery wound
Apple carry out leaching fruit test.Apple is immersed respectively each chemicals treatment (30% 1000 times of probenazole procymidone microemulsion,
750 times, 500 times；50% 600 times of carbendazol wettable powder；43% 1500 times of tebuconazole suspension concentrates) and clear water control in
(30% probenazole procymidone microemulsion is prepared by embodiment 5), leaching fruit time are 30s, are subsequently placed in place mat under the tree of field
It dries in the shade in sterilizing filter paper.It then transports Shandong Fruit-tree Inst. back, is pre-chilled for 24 hours, often in freezer (1.0 ± 1.0 DEG C of temperature)
4 repetitions are handled, 50 apples of every repetition are put into PE freshness protection package, and being then neatly put into can have enough to meet the need in plastics fruit packing case,
One repetition of every case, totally 192 case, is finally putting into freezer (1.0 ± 1.0 DEG C of temperature) and is stored.15d, 30d after storage,
A case is taken out in each repetition of 45d, 60d respectively, carries out fruit rotting rate investigation.Processing method is same as above under 20 DEG C of room temperature.
Drug effect statistical analysis technique: pharmacy control efficacy is calculated according to the following formula；It is poor that the new multipole of Duncan is carried out using DPS software
Examine its otherness (P≤0.05).
a.
b.
As a result as shown in table 4 (Fig. 1) (note: in table 4,5, each extension rate refers to quality multiple for solid dosage forms,
For liquid dosage form, refer to volume multiple).
The pharmacodynamic results of 4 30% probenazole procymidone microemulsion of table prevention and treatment storage period apple anthracnose
As shown in Table 4, after fruit processing is soaked after 30% 750 times of probenazole procymidone microemulsion, 500 times are adopted, item is refrigerated
Rotting rate under part when Apples 60d is substantially less than 600 times of 50% carbendazol wettable powder of comparison medicament, 43% Tebuconazole
The rotting rate that 1500 times of suspending agent is significantly better than other each processing to the control efficiency of apple anthracnose.Under room temperature, 25d
When investigation, 30% 1000 times of probenazole procymidone microemulsion, 750 times, 500 times of processing it is aobvious to the control efficiency of apple anthracnose
It writes better than other each processing.Therefore, 30% probenazole procymidone microemulsion leaching fruit processing apple, inhibits in storage after adopting
The generation of apple anthrax bacteria improves the commodity value of fruit to extend storage period.
Application example 2:30% probenazole procymidone suspending agent prevents and treats the gray mold research of storage period garlic stems
On May 4th, 2017 is tested in Jinxiang County town Yu Shan dedicated freezer of peasant household's garlic stems.Before garlic stems harvesting storage, in
800 times of 30% probenazole procymidone suspending agent (preparation of embodiment 3), 600 times, 400 times, 25% 250 times of prochloraz aqueous emulsion,
Dipping garlic stems tongue fur tip 30s in clear water control, is put in storage storage after draining away the water.After garlic stems is cooled to -0.3 DEG C ± 0.2 DEG C in advance, it is packed into
Silicon window bag storage, the normal freezer management together with other garlic stems.The experimental observation phase is 240 days.4 repetitions of each processing, each
Repeat 2 bags (every bag of about 19kg garlic stems).Garlic stems rotting rate, the tongue fur tip are investigated respectively at 90d, 120d, 150d, 180d, 210d, 240d
The indices such as color.
a.
b.
The results are shown in Table 5.
The pharmacodynamic test result of 5 30% probenazole procymidone suspending agent of table prevention and treatment garlic stems gray mold
As shown in Table 5, after slightly processing is soaked after 800 times, 600 times, 400 times of 30% probenazole procymidone suspending agent are adopted,
Garlic stems stores 240d control efficiency 87% or more, and tongue fur slightly color is normal, no phenomenon of losing water, shows good anti-corrosion and protects
Fresh effect is significantly better than 250 times of 25% prochloraz aqueous emulsion.Therefore, it after garlic stems is adopted, suspends using 30% probenazole procymidone
Agent dipping, spraying tongue fur slightly, can significantly inhibit the generation of gray mold in garlic stems storage, to extend storage period, improve storage
Commodity.
Above-described embodiment is provided to those skilled in the art, how to implement and use to be advocated with full disclosure and description
Embodiment, rather than for limiting range disclosed herein.Obvious modification will to those skilled in the art
Within the scope of the appended claims.The all publications, patents and patent applications of this specification citation are incorporated by reference into this
Text, as these publications, patents and patent applications respectively show particularly and individually to be incorporated herein by reference.
Claims (10)
1. the bactericidal composition of a kind of probenazole-containing and procymidone, it is characterised in that: effective component is probenazole and procymidone, thiophene
The weight ratio of bacterium spirit and procymidone is 15:1~1:10.
2. the bactericidal composition of probenazole-containing according to claim 1 and procymidone, it is characterised in that: the probenazole with
The weight ratio of procymidone is 10:1~1:5, further preferred 5:1~1:3, still further preferably 3:1~1:2.
3. the bactericidal composition of probenazole-containing according to claim 1 or 2 and procymidone, it is characterised in that: described to contain thiophene
In the bactericidal composition of bacterium spirit and procymidone containing allow in Pesticide Science using with acceptable auxiliary material.
4. the bactericidal composition of probenazole-containing according to claim 3 and procymidone, it is characterised in that: the probenazole-containing
It is grouped as with the bactericidal composition of procymidone by the group of following parts by weight: probenazole 1%~80%, procymidone 1%~80%,
Surplus be Pesticide Science in allow using with acceptable auxiliary material.
5. the bactericidal composition of probenazole-containing according to claim 3 or 4 and procymidone, it is characterised in that: the pesticide
Allow to be selected from solvent, dispersing agent, diffusant, disintegrating agent, emulsifier, adsorbent, stabilization using with acceptable auxiliary material in
Agent, wetting agent, defoaming agent, antifreeze etc. or other stabilizations for facilitating effective component drug effect and performance allow using auxiliary agent.
6. the bactericidal composition of probenazole-containing according to any one of claims 1 to 5 and procymidone, it is characterised in that:
The dosage form of the bactericidal composition of the probenazole-containing and procymidone be wettable powder, suspending agent, aqueous emulsion, water dispersible granules or
Microemulsion.
7. the bactericidal composition of probenazole-containing according to claim 5 and procymidone, it is characterised in that: the Bactericidal composite
The dosage form of object is aqueous emulsion, is grouped as by the group of following weight percent:
Or: the dosage form of the bactericidal composition is water dispersible granules, is grouped as by the group of following weight percent:
Or: the dosage form of the bactericidal composition is suspending agent, is grouped as by the group of following weight percent:
Or: the dosage form of the bactericidal composition is wettable powder, is grouped as by the group of following weight percent:
Or: the dosage form of the bactericidal composition is microemulsion, is grouped as by the group of following weight percent:
8. the bactericidal composition of probenazole-containing according to any one of claims 1 to 7 and procymidone is in prevention and treatment water fruits and vegetables storage
Application in hiding phase disease.
9. application according to claim 8, it is characterised in that: the disease is selected from gray mold, anthracnose, ring spot, blueness
Mildew.
10. application according to claim 8 or claim 9, it is characterised in that: when preventing and treating the pathogen of Botrytis cinerea, probenazole is mould with corruption
Both sharp weight ratio is preferred with 10:1~1:3, and more preferably, 1:1 is best by 2:1~1:1；When preventing and treating Botryosphaeria berengeriana f. sp, thiophene bacterium
Spirit and the weight ratio of both procymidones are preferred with 5:1~1:3, and more preferably, 3:1 is best by 3:1~1:1；In prevention and treatment apple anthrax bacteria
When, the weight ratio of probenazole and both procymidones is preferred with 5:1~1:5, and more preferably, 1:1 is best by 2:1~1:2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910122374.3A CN109645112A (en) | 2019-02-19 | 2019-02-19 | A kind of bactericidal composition and its application of probenazole-containing and procymidone |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910122374.3A CN109645112A (en) | 2019-02-19 | 2019-02-19 | A kind of bactericidal composition and its application of probenazole-containing and procymidone |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109645112A true CN109645112A (en) | 2019-04-19 |
Family
ID=66123033
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910122374.3A Pending CN109645112A (en) | 2019-02-19 | 2019-02-19 | A kind of bactericidal composition and its application of probenazole-containing and procymidone |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109645112A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113729074A (en) * | 2021-10-11 | 2021-12-03 | 安徽农业大学 | Preservative for preventing and treating anthracnose of pears and application of preservative |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1559214A (en) * | 2004-02-23 | 2005-01-05 | 如东县植保站 | Caijunke-pesticide for preventing and controlling diseases of crops |
| CN101379993A (en) * | 2008-08-06 | 2009-03-11 | 张少武 | Bactericidal water dispersion granule of triabendazole |
| CN102047879A (en) * | 2009-10-27 | 2011-05-11 | 杨艳芬 | Preparation method of procymidone suspending agent |
| CN103478155A (en) * | 2013-09-17 | 2014-01-01 | 南京通泽农业科技有限公司 | Wax gourd sclerotinia preventing and treating method |
| CN109662098A (en) * | 2019-02-19 | 2019-04-23 | 山东省果树研究所 | A kind of bactericidal composition and its application of probenazole-containing and polyoxin |
-
2019
- 2019-02-19 CN CN201910122374.3A patent/CN109645112A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1559214A (en) * | 2004-02-23 | 2005-01-05 | 如东县植保站 | Caijunke-pesticide for preventing and controlling diseases of crops |
| CN101379993A (en) * | 2008-08-06 | 2009-03-11 | 张少武 | Bactericidal water dispersion granule of triabendazole |
| CN102047879A (en) * | 2009-10-27 | 2011-05-11 | 杨艳芬 | Preparation method of procymidone suspending agent |
| CN103478155A (en) * | 2013-09-17 | 2014-01-01 | 南京通泽农业科技有限公司 | Wax gourd sclerotinia preventing and treating method |
| CN109662098A (en) * | 2019-02-19 | 2019-04-23 | 山东省果树研究所 | A kind of bactericidal composition and its application of probenazole-containing and polyoxin |
Non-Patent Citations (2)
| Title |
|---|
| 全国农业技术推广服务中心: "《无公害农产品适用农药品种应用指南》", 31 October 2007, 中国农业出版社 * |
| 高微微: "《常用中草药病虫害防治手册》", 31 January 2004, 中国农业出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113729074A (en) * | 2021-10-11 | 2021-12-03 | 安徽农业大学 | Preservative for preventing and treating anthracnose of pears and application of preservative |
| CN113729074B (en) * | 2021-10-11 | 2023-06-23 | 安徽农业大学 | Preservative for preventing and treating pear anthracnose and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20240180155A1 (en) | Antimicrobial Compositions and Related Methods of Use | |
| CN101965861B (en) | Method for prolonging refreshing time of Chinese pears | |
| CN105454273B (en) | A kind of microbicide compositions | |
| CN105454301B (en) | The method for reducing postharvest fruit and vegetable storage disease using compound drug | |
| CN104686561B (en) | Microcapsule formulation containing allyl group isosulfocyanate and preparation method and application | |
| CN104012647A (en) | Loquat fruit preservation method through nano packaging compound plant essential oil treatment | |
| CN112155016B (en) | Isothiocyanate and fluopyram composition, preparation and application | |
| CN107668194A (en) | Fludioxonil combines application of the chitosan oligosaccharide in preventing and treating fruit, vegetable storage disease | |
| CN105917991A (en) | Antistaling method for grape | |
| CN109645112A (en) | A kind of bactericidal composition and its application of probenazole-containing and procymidone | |
| CN101961021A (en) | Fluopyram-containing bactericidal composition | |
| CN87102017A (en) | Fresh-preserving agent for oranges and tangerines | |
| CN106857599A (en) | A kind of bactericidal composition containing pyraclostrobin and folpet and its application | |
| CN109662098A (en) | A kind of bactericidal composition and its application of probenazole-containing and polyoxin | |
| CN104782747B (en) | A kind of preservation method of mulberry fruit | |
| CN106819090A (en) | A kind of fruit and vegetable fresh-keeping agent containing amino-oligosaccharide and Tea Polyphenols | |
| CN109730076A (en) | A kind of bactericidal composition and its application containing difenoconazole and pyrimethanil | |
| CN107668195A (en) | Fludioxonil combines application of the cinnamic acid in preventing and treating fruit, vegetable storage disease | |
| CN107494710A (en) | Lentinan combines application of the fludioxonil in preventing and treating fruit, vegetable storage disease | |
| CN103651772B (en) | Sterilization composition | |
| CN108283215A (en) | The dispersible solid preparation fresh-keeping for fruit antisepsis and its preparation method and application | |
| LU102718B1 (en) | Pre-harvested Fruit microbial inhibition Preservative Agent, Post-harvested Fruit Ethylene Inhibitory Preservative Agent and Combined Application Method Thereof | |
| CN108157373A (en) | A kind of biogas slurry for preventing crop gray mold and bactericide compounded composition and its application | |
| CN106819092A (en) | A kind of pears antistaling agent | |
| CN109907051B (en) | Insecticidal composition containing fluazinam and propargite |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190419 |